AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51880.t1 MTR_7g085490 97.794 136 3 0 1 136 155 290 9.57e-93 277
MS.gene51880.t1 MTR_7g085470 89.167 120 6 1 134 246 201 320 7.61e-72 222
MS.gene51880.t1 MTR_7g085570 69.149 94 27 1 155 246 192 285 4.70e-37 132
MS.gene51880.t1 MTR_4g010050 58.491 106 26 3 134 226 225 325 1.17e-28 113
MS.gene51880.t1 MTR_2g104050 60.440 91 24 2 134 217 225 310 2.91e-26 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene51880.t1 AT1G64770 62.590 139 50 1 1 139 150 286 8.95e-55 178
MS.gene51880.t1 AT1G64770 62.774 137 49 1 1 137 150 284 3.30e-54 179
MS.gene51880.t1 AT1G64770 63.235 136 48 1 1 136 150 283 3.51e-54 178
MS.gene51880.t1 AT1G48900 60.377 106 24 3 134 226 225 325 3.90e-31 119
MS.gene51880.t1 AT1G48900 65.934 91 19 2 134 217 225 310 5.49e-31 119
MS.gene51880.t1 AT1G15310 50.833 120 43 4 134 246 225 335 1.90e-28 112
MS.gene51880.t1 AT5G49500 59.341 91 25 2 134 217 105 190 2.77e-26 105
MS.gene51880.t1 AT5G49500 59.341 91 25 2 134 217 227 312 5.10e-26 105

Find 58 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCCTTTGTTCTTTCTT+TGG 0.192458 7.4:-21850899 None:intergenic
CTCTTCTCTTAAATCTAAAA+TGG 0.228276 7.4:-21850654 None:intergenic
CCGTTTGTTAGTCGTCTATT+AGG 0.257109 7.4:+21851590 MS.gene51880:CDS
AGCTGTAATTGTTTCTAAAA+TGG 0.274828 7.4:+21851291 MS.gene51880:CDS
ATAGCATTGGTTAGAGTAAC+AGG 0.282402 7.4:-21850625 None:intergenic
CATGAACCTGGCTTCAATTC+AGG 0.299182 7.4:-21850811 None:intergenic
GGCAGGTTTAGAGGCAATTC+AGG 0.299728 7.4:-21851656 None:intergenic
AAAGCCTTTGTTCTTTCTTT+GGG 0.310843 7.4:-21850898 None:intergenic
TTTCTTTCTGGCAGGTTTAG+AGG 0.311232 7.4:-21851665 None:intergenic
TTGGTTAGAGTAACAGGTTT+TGG 0.328743 7.4:-21850619 None:intergenic
TTATTCTCAAGGAGAGTAAT+AGG 0.341075 7.4:-21850850 None:intergenic
AGAGGTGGTGCGGCAATTAA+AGG 0.342398 7.4:+21850673 MS.gene51880:CDS
TCTCAAAGGTTGATTTCATT+TGG 0.368709 7.4:+21850781 MS.gene51880:CDS
GATGGAGTCAAGATTTGAAA+AGG 0.400664 7.4:-21850739 None:intergenic
AAAATATGAAATCATTGATC+AGG 0.416361 7.4:+21850939 MS.gene51880:CDS
GTCTATTAGGCATGGATGAC+TGG 0.428294 7.4:+21851603 MS.gene51880:CDS
AAGAGTCCGGTCATATTCAT+AGG 0.428991 7.4:+21851524 MS.gene51880:CDS
CATAGGAACTGGTGAACATA+TGG 0.442484 7.4:+21851541 MS.gene51880:CDS
GATGGCTATGCCATGGGTGG+TGG 0.461610 7.4:+21851313 MS.gene51880:CDS
CCGGTCATATTCATAGGAAC+TGG 0.464779 7.4:+21851530 MS.gene51880:CDS
GCCTTTGTTCTTTCTTTGGG+AGG 0.467470 7.4:-21850895 None:intergenic
CTGAATTGAAGCCAGGTTCA+TGG 0.470856 7.4:+21850812 MS.gene51880:CDS
ATGGAGTCAAGATTTGAAAA+GGG 0.482931 7.4:-21850738 None:intergenic
TTGCCATGCTCACAGGATAG+AGG 0.487005 7.4:-21850570 None:intergenic
TGATCATTGTAGTCGTAAGC+AGG 0.494522 7.4:+21851237 MS.gene51880:CDS
CCTTGTTGAGTCCATGAACC+TGG 0.507008 7.4:-21850823 None:intergenic
GTAATTGTTTCTAAAATGGA+TGG 0.523200 7.4:+21851295 MS.gene51880:CDS
TGTTAGTCGTCTATTAGGCA+TGG 0.525929 7.4:+21851595 MS.gene51880:CDS
ACTGCTGTTGCCATGCTCAC+AGG 0.530706 7.4:-21850577 None:intergenic
TGAAATCAACCTTTGAGACT+CGG 0.532325 7.4:-21850776 None:intergenic
GAAAAGGGGAAGCAAGAGGA+GGG 0.532333 7.4:-21850723 None:intergenic
AAAATGGCTTAATATAGCAT+TGG 0.535095 7.4:-21850638 None:intergenic
TTTACACATAGTGTTGACAT+TGG 0.544116 7.4:+21851268 MS.gene51880:CDS
TGTTGCAGCAACAAAGAGTC+CGG 0.545682 7.4:+21851511 MS.gene51880:intron
GCTGAGCCTGAATTGAAGCC+AGG 0.546842 7.4:+21850805 MS.gene51880:CDS
TTTAGATTTAAGAGAAGAGG+TGG 0.556422 7.4:+21850658 MS.gene51880:CDS
CCTAATAGACGACTAACAAA+CGG 0.558547 7.4:-21851590 None:intergenic
AGGGTGTGAGCAGTATGAGC+AGG 0.560744 7.4:-21850704 None:intergenic
CAATGAAAACCGAGTCTCAA+AGG 0.560884 7.4:+21850767 MS.gene51880:CDS
CTCTACTCATCTTATTCTCA+AGG 0.565176 7.4:-21850861 None:intergenic
TGAAAAGGGGAAGCAAGAGG+AGG 0.568175 7.4:-21850724 None:intergenic
TGCCATGCTCACAGGATAGA+GGG 0.569663 7.4:-21850569 None:intergenic
GTCCCTCTATCCTGTGAGCA+TGG 0.570423 7.4:+21850567 MS.gene51880:CDS
ACAAGTGGTGCAACGATGGA+GGG 0.576239 7.4:-21851715 None:intergenic
AACAAGTGGTGCAACGATGG+AGG 0.576961 7.4:-21851716 None:intergenic
CCAGTTCCTATGAATATGAC+CGG 0.578609 7.4:-21851530 None:intergenic
TCAAACAAGTGGTGCAACGA+TGG 0.579389 7.4:-21851719 None:intergenic
CCAGGTTCATGGACTCAACA+AGG 0.611546 7.4:+21850823 MS.gene51880:CDS
CTTACGACTACAATGATCAA+AGG 0.629516 7.4:-21851233 None:intergenic
GCTAAGAGAACCACCACCCA+TGG 0.629930 7.4:-21851323 None:intergenic
AAAATGGATGGCTATGCCAT+GGG 0.631223 7.4:+21851307 MS.gene51880:CDS
TGGAGTCAAGATTTGAAAAG+GGG 0.631264 7.4:-21850737 None:intergenic
TAAAATGGATGGCTATGCCA+TGG 0.631944 7.4:+21851306 MS.gene51880:CDS
ATTTGAAAAGGGGAAGCAAG+AGG 0.647512 7.4:-21850727 None:intergenic
ACCTCCCAAAGAAAGAACAA+AGG 0.664272 7.4:+21850894 MS.gene51880:CDS
ATTTAAGAGAAGAGGTGGTG+CGG 0.665156 7.4:+21850663 MS.gene51880:CDS
GAAGGTCTCAGTCAAACAAG+TGG 0.673464 7.4:-21851730 None:intergenic
ATGGATGGCTATGCCATGGG+TGG 0.678999 7.4:+21851310 MS.gene51880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTTTATGTGTTTT+GGG + chr7.4:21851455-21851474 MS.gene51880:intron 10.0%
!!! TATATTTTTTTTATGTGTTT+TGG + chr7.4:21851454-21851473 MS.gene51880:intron 10.0%
!! AAAATATGAAATCATTGATC+AGG + chr7.4:21850939-21850958 MS.gene51880:CDS 20.0%
!!! TCAACAATTGAATTTTTTAG+CGG - chr7.4:21851388-21851407 None:intergenic 20.0%
!!! TCAATGATTTCATATTTTGA+AGG - chr7.4:21850937-21850956 None:intergenic 20.0%
! AAAATGGCTTAATATAGCAT+TGG - chr7.4:21850641-21850660 None:intergenic 25.0%
! CTCTTCTCTTAAATCTAAAA+TGG - chr7.4:21850657-21850676 None:intergenic 25.0%
!! AACAAGATCTAGAACTTAAT+CGG - chr7.4:21851179-21851198 None:intergenic 25.0%
!! CATATAAGATGATTTGTGAT+TGG + chr7.4:21851043-21851062 MS.gene51880:intron 25.0%
!! CATTTTAGATTTAAGAGAAG+AGG + chr7.4:21850655-21850674 MS.gene51880:CDS 25.0%
!!! AAATCGTTCTAGAACTAAAT+CGG - chr7.4:21851110-21851129 None:intergenic 25.0%
!!! AGCTGTAATTGTTTCTAAAA+TGG + chr7.4:21851291-21851310 MS.gene51880:CDS 25.0%
!!! AGTTCTAGATCTTGTTATAT+TGG + chr7.4:21851183-21851202 MS.gene51880:intron 25.0%
!!! ATGATTTCATATTTTGAAGG+CGG - chr7.4:21850934-21850953 None:intergenic 25.0%
!!! GTAATTGTTTCTAAAATGGA+TGG + chr7.4:21851295-21851314 MS.gene51880:CDS 25.0%
!!! TCTCTTACTTTAAAGTGAAT+TGG - chr7.4:21851359-21851378 None:intergenic 25.0%
AAAAGCCTTTGTTCTTTCTT+TGG - chr7.4:21850902-21850921 None:intergenic 30.0%
AAAGCCTTTGTTCTTTCTTT+GGG - chr7.4:21850901-21850920 None:intergenic 30.0%
ATGGAGTCAAGATTTGAAAA+GGG - chr7.4:21850741-21850760 None:intergenic 30.0%
TTATTCTCAAGGAGAGTAAT+AGG - chr7.4:21850853-21850872 None:intergenic 30.0%
TTTAGATTTAAGAGAAGAGG+TGG + chr7.4:21850658-21850677 MS.gene51880:CDS 30.0%
! CTTGTTATATTGGTTATCGA+TGG + chr7.4:21851193-21851212 MS.gene51880:intron 30.0%
! TCTCAAAGGTTGATTTCATT+TGG + chr7.4:21850781-21850800 MS.gene51880:CDS 30.0%
!! TATGTGTTTTGGGTACATAT+TGG + chr7.4:21851465-21851484 MS.gene51880:intron 30.0%
!! TTCTAGAACGATTTACATTG+TGG + chr7.4:21851116-21851135 MS.gene51880:intron 30.0%
!! TTTACACATAGTGTTGACAT+TGG + chr7.4:21851268-21851287 MS.gene51880:CDS 30.0%
CCTAATAGACGACTAACAAA+CGG - chr7.4:21851593-21851612 None:intergenic 35.0%
CTCTACTCATCTTATTCTCA+AGG - chr7.4:21850864-21850883 None:intergenic 35.0%
CTTACGACTACAATGATCAA+AGG - chr7.4:21851236-21851255 None:intergenic 35.0%
GATGGAGTCAAGATTTGAAA+AGG - chr7.4:21850742-21850761 None:intergenic 35.0%
TGGAGTCAAGATTTGAAAAG+GGG - chr7.4:21850740-21850759 None:intergenic 35.0%
TTGGTTATCGATGGCAATAT+TGG + chr7.4:21851202-21851221 MS.gene51880:intron 35.0%
! TTGGTTAGAGTAACAGGTTT+TGG - chr7.4:21850622-21850641 None:intergenic 35.0%
!! ATAGCATTGGTTAGAGTAAC+AGG - chr7.4:21850628-21850647 None:intergenic 35.0%
!! TGAAATCAACCTTTGAGACT+CGG - chr7.4:21850779-21850798 None:intergenic 35.0%
AAAATGGATGGCTATGCCAT+GGG + chr7.4:21851307-21851326 MS.gene51880:CDS 40.0%
AAGAGTCCGGTCATATTCAT+AGG + chr7.4:21851524-21851543 MS.gene51880:CDS 40.0%
ACCTCCCAAAGAAAGAACAA+AGG + chr7.4:21850894-21850913 MS.gene51880:CDS 40.0%
ACCTGCCAGAAAGAAAAATC+TGG + chr7.4:21851672-21851691 MS.gene51880:CDS 40.0%
ATTTAAGAGAAGAGGTGGTG+CGG + chr7.4:21850663-21850682 MS.gene51880:CDS 40.0%
ATTTGAAAAGGGGAAGCAAG+AGG - chr7.4:21850730-21850749 None:intergenic 40.0%
CAATGAAAACCGAGTCTCAA+AGG + chr7.4:21850767-21850786 MS.gene51880:CDS 40.0%
CATAGGAACTGGTGAACATA+TGG + chr7.4:21851541-21851560 MS.gene51880:CDS 40.0%
CCAGTTCCTATGAATATGAC+CGG - chr7.4:21851533-21851552 None:intergenic 40.0%
TAAAATGGATGGCTATGCCA+TGG + chr7.4:21851306-21851325 MS.gene51880:CDS 40.0%
TAGTCTGTGTCCACAATGTT+TGG - chr7.4:21851150-21851169 None:intergenic 40.0%
TGATCATTGTAGTCGTAAGC+AGG + chr7.4:21851237-21851256 MS.gene51880:CDS 40.0%
TGTTAGTCGTCTATTAGGCA+TGG + chr7.4:21851595-21851614 MS.gene51880:CDS 40.0%
! CCGTTTGTTAGTCGTCTATT+AGG + chr7.4:21851590-21851609 MS.gene51880:CDS 40.0%
! GGCATCCAGATTTTTCTTTC+TGG - chr7.4:21851680-21851699 None:intergenic 40.0%
! TCGGTTTTCATTGCTTCTGA+TGG - chr7.4:21850760-21850779 None:intergenic 40.0%
! TTTCTTTCTGGCAGGTTTAG+AGG - chr7.4:21851668-21851687 None:intergenic 40.0%
!! TCCAGATTTTTCTTTCTGGC+AGG - chr7.4:21851676-21851695 None:intergenic 40.0%
CATGAACCTGGCTTCAATTC+AGG - chr7.4:21850814-21850833 None:intergenic 45.0%
CCGGTCATATTCATAGGAAC+TGG + chr7.4:21851530-21851549 MS.gene51880:CDS 45.0%
CTGAATTGAAGCCAGGTTCA+TGG + chr7.4:21850812-21850831 MS.gene51880:CDS 45.0%
GCCTTTGTTCTTTCTTTGGG+AGG - chr7.4:21850898-21850917 None:intergenic 45.0%
GGACAAGTGACCAAACATTG+TGG + chr7.4:21851137-21851156 MS.gene51880:intron 45.0%
GTCTATTAGGCATGGATGAC+TGG + chr7.4:21851603-21851622 MS.gene51880:CDS 45.0%
TCAAACAAGTGGTGCAACGA+TGG - chr7.4:21851722-21851741 None:intergenic 45.0%
TGTTGCAGCAACAAAGAGTC+CGG + chr7.4:21851511-21851530 MS.gene51880:intron 45.0%
AACAAGTGGTGCAACGATGG+AGG - chr7.4:21851719-21851738 None:intergenic 50.0%
ACAAGTGGTGCAACGATGGA+GGG - chr7.4:21851718-21851737 None:intergenic 50.0%
AGAGGTGGTGCGGCAATTAA+AGG + chr7.4:21850673-21850692 MS.gene51880:CDS 50.0%
CCAGGTTCATGGACTCAACA+AGG + chr7.4:21850823-21850842 MS.gene51880:CDS 50.0%
CCTTGTTGAGTCCATGAACC+TGG - chr7.4:21850826-21850845 None:intergenic 50.0%
GAAAAGGGGAAGCAAGAGGA+GGG - chr7.4:21850726-21850745 None:intergenic 50.0%
GGCAGGTTTAGAGGCAATTC+AGG - chr7.4:21851659-21851678 None:intergenic 50.0%
TGAAAAGGGGAAGCAAGAGG+AGG - chr7.4:21850727-21850746 None:intergenic 50.0%
TGCCATGCTCACAGGATAGA+GGG - chr7.4:21850572-21850591 None:intergenic 50.0%
TTGCCATGCTCACAGGATAG+AGG - chr7.4:21850573-21850592 None:intergenic 50.0%
ACTGCTGTTGCCATGCTCAC+AGG - chr7.4:21850580-21850599 None:intergenic 55.0%
AGGGTGTGAGCAGTATGAGC+AGG - chr7.4:21850707-21850726 None:intergenic 55.0%
ATGGATGGCTATGCCATGGG+TGG + chr7.4:21851310-21851329 MS.gene51880:CDS 55.0%
GATGGAGGGAAAAACGCACG+TGG - chr7.4:21851704-21851723 None:intergenic 55.0%
GCTAAGAGAACCACCACCCA+TGG - chr7.4:21851326-21851345 None:intergenic 55.0%
GCTGAGCCTGAATTGAAGCC+AGG + chr7.4:21850805-21850824 MS.gene51880:CDS 55.0%
GTCCCTCTATCCTGTGAGCA+TGG + chr7.4:21850567-21850586 MS.gene51880:CDS 55.0%
GATGGCTATGCCATGGGTGG+TGG + chr7.4:21851313-21851332 MS.gene51880:CDS 60.0%
GGAGGGAAAAACGCACGTGG+AGG - chr7.4:21851701-21851720 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 21850553 21851745 21850553 ID=MS.gene51880
chr7.4 mRNA 21850553 21851745 21850553 ID=MS.gene51880.t1;Parent=MS.gene51880
chr7.4 exon 21850553 21850960 21850553 ID=MS.gene51880.t1.exon1;Parent=MS.gene51880.t1
chr7.4 CDS 21850553 21850960 21850553 ID=cds.MS.gene51880.t1;Parent=MS.gene51880.t1
chr7.4 exon 21851229 21851334 21851229 ID=MS.gene51880.t1.exon2;Parent=MS.gene51880.t1
chr7.4 CDS 21851229 21851334 21851229 ID=cds.MS.gene51880.t1;Parent=MS.gene51880.t1
chr7.4 exon 21851519 21851745 21851519 ID=MS.gene51880.t1.exon3;Parent=MS.gene51880.t1
chr7.4 CDS 21851519 21851745 21851519 ID=cds.MS.gene51880.t1;Parent=MS.gene51880.t1
Gene Sequence

>MS.gene51880

ATGACTTATATCGTGTCCCTCTATCCTGTGAGCATGGCAACAGCAGTTATAGTTAAGAACAAATCTCCAAAACCTGTTACTCTAACCAATGCTATATTAAGCCATTTTAGATTTAAGAGAAGAGGTGGTGCGGCAATTAAAGGTCTTCAAACCTGCTCATACTGCTCACACCCTCCTCTTGCTTCCCCTTTTCAAATCTTGACTCCATCAGAAGCAATGAAAACCGAGTCTCAAAGGTTGATTTCATTTGGTGCTGAGCCTGAATTGAAGCCAGGTTCATGGACTCAACAAGGTGTTCCTATTACTCTCCTTGAGAATAAGATGAGTAGAGTTTTTGCTGCACCTCCCAAAGAAAGAACAAAGGCTTTTTATAACACACCGCCTTCAAAATATGAAATCATTGATCAGGTTAGAATGACGAGTTTCATTTTGATACAACACTTTTTACACAGTCAAACAATCATACTTTGAGTCTAATTACTCAAAGTCACATATAAGATGATTTGTGATTGGTTGATTGATAGTGTAAATGATCTTTACACAGACTATGCGTACCGATTTAGTTCTAGAACGATTTACATTGTGGACAAGTGACCAAACATTGTGGACACAGACTATGCGTACCGATTAAGTTCTAGATCTTGTTATATTGGTTATCGATGGCAATATTGGTCAGCATGCCTTTGATCATTGTAGTCGTAAGCAGGCAACAGCATTTACACATAGTGTTGACATTGGAGCTGTAATTGTTTCTAAAATGGATGGCTATGCCATGGGTGGTGGTTCTCTTAGCGCATAAGTTACCAATTCACTTTAAAGTAAGAGATCTTAACCGCTAAAAAATTCAATTGTTGATATTACACGAATAACAATTGAATTGCTTAAAATGTATTCACTTAGATATATTTTTTTTATGTGTTTTGGGTACATATTGGTCTTATTAATGTTATATGTGCAGTGTTGCAGCAACAAAGAGTCCGGTCATATTCATAGGAACTGGTGAACATATGGATGAGTTTGAAGTTTTTGAAGTTAAGCCGTTTGTTAGTCGTCTATTAGGCATGGATGACTGGTATGAGTTTATTGACAGAGTTTATGAAGTGCCTGAATTGCCTCTAAACCTGCCAGAAAGAAAAATCTGGATGCCTCCACGTGCGTTTTTCCCTCCATCGTTGCACCACTTGTTTGACTGA

Protein sequence

>MS.gene51880.t1

MTYIVSLYPVSMATAVIVKNKSPKPVTLTNAILSHFRFKRRGGAAIKGLQTCSYCSHPPLASPFQILTPSEAMKTESQRLISFGAEPELKPGSWTQQGVPITLLENKMSRVFAAPPKERTKAFYNTPPSKYEIIDQHAFDHCSRKQATAFTHSVDIGAVIVSKMDGYAMGGATKSPVIFIGTGEHMDEFEVFEVKPFVSRLLGMDDWYEFIDRVYEVPELPLNLPERKIWMPPRAFFPPSLHHLFD