AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82243


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82243.t1 MTR_4g029130 93.182 88 6 0 1 88 1 88 9.07e-55 169
MS.gene82243.t1 MTR_4g029130 92.105 38 3 0 88 125 153 190 1.40e-18 77.4
MS.gene82243.t1 MTR_7g072540 64.045 89 27 2 1 86 1 87 3.36e-31 110
MS.gene82243.t1 MTR_5g083970 59.259 81 26 2 11 85 15 94 6.08e-23 89.4
MS.gene82243.t1 MTR_3g072360 52.439 82 32 1 11 85 19 100 2.45e-13 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 25 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTGCAGTTCCATTTGTT+TGG 0.199775 4.2:-74912342 MS.gene82243:CDS
TGTTGCAGTTCCATTTGTTT+GGG 0.310743 4.2:-74912341 MS.gene82243:CDS
GTCTTTAGAGAATGTGTGTT+TGG 0.374019 4.2:+74912300 None:intergenic
CCAACTTCTACTTTGTGCTT+TGG 0.382263 4.2:-74911968 MS.gene82243:CDS
AATGTGTGTTTGGGAGTTCC+AGG 0.387249 4.2:+74912310 None:intergenic
TCTGAAAGATGGGTGACAAT+TGG 0.414182 4.2:+74912369 None:intergenic
CACGCCTCTTGTAGTTACTT+TGG 0.419541 4.2:+74911912 None:intergenic
GATAAGAAGGTGGAGGTGTA+AGG 0.431589 4.2:+74912266 None:intergenic
GTAACTACAAGAGGCGTGTA+AGG 0.469845 4.2:-74911907 MS.gene82243:CDS
ATAAGAAGGTGGAGGTGTAA+GGG 0.477682 4.2:+74912267 None:intergenic
TCTTTAGAGAATGTGTGTTT+GGG 0.478199 4.2:+74912301 None:intergenic
GCAACAGAAACTCTGAAAGA+TGG 0.487509 4.2:+74912358 None:intergenic
GGTTGAGATTCCCAAACAAA+TGG 0.489006 4.2:+74912331 None:intergenic
TTCAGGGGTGAAAATGAAGA+TGG 0.498666 4.2:-74912001 MS.gene82243:intron
TAAGAAGGTGGAGGTGTAAG+GGG 0.511212 4.2:+74912268 None:intergenic
CCAAAGCACAAAGTAGAAGT+TGG 0.519370 4.2:+74911968 None:intergenic
CAACAGAAACTCTGAAAGAT+GGG 0.528072 4.2:+74912359 None:intergenic
TTTCAGCAGACTACTTTCAA+AGG 0.534157 4.2:-74912398 MS.gene82243:CDS
TTTGTTTGGGAATCTCAACC+TGG 0.562667 4.2:-74912328 MS.gene82243:CDS
CGTTCGATCAGCTCGAACAG+TGG 0.567340 4.2:-74911938 MS.gene82243:CDS
GCATTGTTGAAATGATAAGA+AGG 0.591984 4.2:+74912253 None:intergenic
GAAGGTGGAGGTGTAAGGGG+AGG 0.592725 4.2:+74912271 None:intergenic
GCTTCCAAAGTAACTACAAG+AGG 0.633252 4.2:-74911916 MS.gene82243:CDS
TTGTTGAAATGATAAGAAGG+TGG 0.647192 4.2:+74912256 None:intergenic
TTGAAATGATAAGAAGGTGG+AGG 0.647831 4.2:+74912259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGAAGAAAAATCTTAAGCTT+TGG - chr4.2:74912321-74912340 MS.gene82243:CDS 25.0%
!! AAAATCTTCCAAGTATCTTT+TGG - chr4.2:74912155-74912174 MS.gene82243:intron 25.0%
!!! CTTTTTTTCGTTCTTTTTGT+TGG + chr4.2:74912130-74912149 None:intergenic 25.0%
AGATTCTTCCAAAATTACCT+CGG - chr4.2:74912281-74912300 MS.gene82243:CDS 30.0%
GCATTGTTGAAATGATAAGA+AGG + chr4.2:74912108-74912127 None:intergenic 30.0%
TGCTTCAAAAACTAAACTTC+AGG - chr4.2:74912181-74912200 MS.gene82243:intron 30.0%
TTGTTGAAATGATAAGAAGG+TGG + chr4.2:74912105-74912124 None:intergenic 30.0%
! ATTGATGACAATGAAAACGA+AGG + chr4.2:74912261-74912280 None:intergenic 30.0%
! TCTTTAGAGAATGTGTGTTT+GGG + chr4.2:74912060-74912079 None:intergenic 30.0%
! TTGATGACAATGAAAACGAA+GGG + chr4.2:74912260-74912279 None:intergenic 30.0%
!!! TGGATCTTCTTTTGATTTCA+GGG - chr4.2:74912341-74912360 MS.gene82243:CDS 30.0%
!!! TTGGATCTTCTTTTGATTTC+AGG - chr4.2:74912340-74912359 MS.gene82243:CDS 30.0%
AAGCAATGCCAAAAGATACT+TGG + chr4.2:74912166-74912185 None:intergenic 35.0%
TGTTGCAGTTCCATTTGTTT+GGG - chr4.2:74912017-74912036 MS.gene82243:intron 35.0%
TTGAAATGATAAGAAGGTGG+AGG + chr4.2:74912102-74912121 None:intergenic 35.0%
TTTCAGCAGACTACTTTCAA+AGG - chr4.2:74911960-74911979 MS.gene82243:CDS 35.0%
! CAACAGAAACTCTGAAAGAT+GGG + chr4.2:74912002-74912021 None:intergenic 35.0%
! GTCTTTAGAGAATGTGTGTT+TGG + chr4.2:74912061-74912080 None:intergenic 35.0%
!!! GGATCTTCTTTTGATTTCAG+GGG - chr4.2:74912342-74912361 MS.gene82243:CDS 35.0%
CCAAAGCACAAAGTAGAAGT+TGG + chr4.2:74912393-74912412 None:intergenic 40.0%
CCAACTTCTACTTTGTGCTT+TGG - chr4.2:74912390-74912409 MS.gene82243:CDS 40.0%
CTGTTGCAGTTCCATTTGTT+TGG - chr4.2:74912016-74912035 MS.gene82243:intron 40.0%
GCTTCCAAAGTAACTACAAG+AGG - chr4.2:74912442-74912461 MS.gene82243:CDS 40.0%
GGTTGAGATTCCCAAACAAA+TGG + chr4.2:74912030-74912049 None:intergenic 40.0%
TCTGAAAGATGGGTGACAAT+TGG + chr4.2:74911992-74912011 None:intergenic 40.0%
TTCAGGGGTGAAAATGAAGA+TGG - chr4.2:74912357-74912376 MS.gene82243:CDS 40.0%
TTTGTTTGGGAATCTCAACC+TGG - chr4.2:74912030-74912049 MS.gene82243:intron 40.0%
! ATAAGAAGGTGGAGGTGTAA+GGG + chr4.2:74912094-74912113 None:intergenic 40.0%
! GATTTTTCTTCTGCTAACCC+TGG + chr4.2:74912313-74912332 None:intergenic 40.0%
! GCAACAGAAACTCTGAAAGA+TGG + chr4.2:74912003-74912022 None:intergenic 40.0%
AATGTGTGTTTGGGAGTTCC+AGG + chr4.2:74912051-74912070 None:intergenic 45.0%
CACGCCTCTTGTAGTTACTT+TGG + chr4.2:74912449-74912468 None:intergenic 45.0%
CGATGACAAAGAAGACGATG+AGG + chr4.2:74912232-74912251 None:intergenic 45.0%
! GATAAGAAGGTGGAGGTGTA+AGG + chr4.2:74912095-74912114 None:intergenic 45.0%
! GTAACTACAAGAGGCGTGTA+AGG - chr4.2:74912451-74912470 MS.gene82243:CDS 45.0%
! TAAGAAGGTGGAGGTGTAAG+GGG + chr4.2:74912093-74912112 None:intergenic 45.0%
AAATTACCTCGGAGACGACC+AGG - chr4.2:74912292-74912311 MS.gene82243:CDS 50.0%
AATTACCTCGGAGACGACCA+GGG - chr4.2:74912293-74912312 MS.gene82243:CDS 50.0%
! GGTCGTCTCCGAGGTAATTT+TGG + chr4.2:74912292-74912311 None:intergenic 50.0%
AGACGATGAGGACGAAGACG+AGG + chr4.2:74912220-74912239 None:intergenic 55.0%
CGTTCGATCAGCTCGAACAG+TGG - chr4.2:74912420-74912439 MS.gene82243:CDS 55.0%
! GAAGGTGGAGGTGTAAGGGG+AGG + chr4.2:74912090-74912109 None:intergenic 60.0%
GCTAACCCTGGTCGTCTCCG+AGG + chr4.2:74912301-74912320 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 74911904 74912476 74911904 ID=MS.gene82243
chr4.2 mRNA 74911904 74912476 74911904 ID=MS.gene82243.t1;Parent=MS.gene82243
chr4.2 exon 74912214 74912476 74912214 ID=MS.gene82243.t1.exon1;Parent=MS.gene82243.t1
chr4.2 CDS 74912214 74912476 74912214 ID=cds.MS.gene82243.t1;Parent=MS.gene82243.t1
chr4.2 exon 74911904 74912018 74911904 ID=MS.gene82243.t1.exon2;Parent=MS.gene82243.t1
chr4.2 CDS 74911904 74912018 74911904 ID=cds.MS.gene82243.t1;Parent=MS.gene82243.t1
Gene Sequence

>MS.gene82243

ATGTTCAATTCAACATGTGAGAAGAGTGTTCTTCAAATCAAACAAGATGACAAGTTTTTCAGCAGACTACTTTCAAAGGAAAATTCCAATTGTCACCCATCTTTCAGAGTTTCTGTTGCAGTTCCATTTGTTTGGGAATCTCAACCTGGAACTCCCAAACACACATTCTCTAAAGACATTATTCCTCCCCTTACACCTCCACCTTCTTATCATTTCAACAATGCCAACAAAAAGAACGAAAAAAAGCACTCAAAATCTTCCAAGTATCTTTTGGCATTGCTTCAAAAACTAAACTTCAGGAAGAACAATCAGTCCTCGTCTTCGTCCTCATCGTCTTCTTTGTCATCGTCATACCCTTCGTTTTCATTGTCATCAATAGATTCTTCCAAAATTACCTCGGAGACGACCAGGGTTAGCAGAAGAAAAATCTTAAGCTTTGGATCTTCTTTTGATTTCAGGGGTGAAAATGAAGATGGAAATGATTCACCAACTTCTACTTTGTGCTTTGGAATTCATCGTTCGATCAGCTCGAACAGTGGCTTCCAAAGTAACTACAAGAGGCGTGTAAGGTAA

Protein sequence

>MS.gene82243.t1

MFNSTCEKSVLQIKQDDKFFSRLLSKENSNCHPSFRVSVAVPFVWESQPGTPKHTFSKDIIPPLTPPPSYHFNNANKKNEKKHSKSSKGENEDGNDSPTSTLCFGIHRSISSNSGFQSNYKRRVR