AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72784


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72784.t1 MTR_5g026290 92.593 135 9 1 1 134 1 135 1.96e-90 258
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTTGTACTTTGTTAATT+TGG 0.112303 5.1:+16853478 None:intergenic
CTTTGTTAATTTGGAATAAC+TGG 0.189219 5.1:+16853487 None:intergenic
TCTTTGAAGTTGCTTTCTTC+AGG 0.231770 5.1:+16853733 None:intergenic
ATGCATTGTGCAGCTTCTTA+TGG 0.341347 5.1:-16853832 MS.gene72784:CDS
AAGGGGTCATCACATGAAAA+AGG 0.380796 5.1:-16853700 MS.gene72784:CDS
CTGCACAATGCATTAGTGTT+TGG 0.388403 5.1:+16853842 None:intergenic
CTTTGAAGTTGCTTTCTTCA+GGG 0.422990 5.1:+16853734 None:intergenic
GCACAAAAGCTTCACAGGTT+GGG 0.439585 5.1:-16853649 MS.gene72784:CDS
CTCAAGCACAAAAGCTTCAC+AGG 0.495602 5.1:-16853654 MS.gene72784:CDS
GCAGCTTCTTATGGTGAAAG+AGG 0.521502 5.1:-16853823 MS.gene72784:CDS
AGCACAAAAGCTTCACAGGT+TGG 0.529568 5.1:-16853650 MS.gene72784:CDS
TGCTAGTGAATCAAGGATGA+TGG 0.543307 5.1:-16853548 MS.gene72784:CDS
CCATCTCGTCGCAAAAGCTA+TGG 0.544204 5.1:-16853787 MS.gene72784:CDS
TTAATTTGGAATAACTGGAA+CGG 0.567626 5.1:+16853492 None:intergenic
GAGATGTTGCTAGTGAATCA+AGG 0.609919 5.1:-16853555 MS.gene72784:CDS
GCTAGTGAATCAAGGATGAT+GGG 0.613297 5.1:-16853547 MS.gene72784:CDS
ATTCACTAGCAACATCTCCA+AGG 0.613391 5.1:+16853560 None:intergenic
GATGGGAATTGATGCACCAA+TGG 0.622941 5.1:-16853530 MS.gene72784:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAAAGAACAAAAAAACAA+GGG - chr5.1:16853648-16853667 MS.gene72784:CDS 20.0%
!!! TTTTCTTTACATGGTAATTA+AGG - chr5.1:16853761-16853780 MS.gene72784:CDS 20.0%
! CTTCAAAGAACAAAAAAACA+AGG - chr5.1:16853647-16853666 MS.gene72784:CDS 25.0%
! TCAAAGAACAAAAAAACAAG+GGG - chr5.1:16853649-16853668 MS.gene72784:CDS 25.0%
!! TTAATTTGGAATAACTGGAA+CGG + chr5.1:16853877-16853896 None:intergenic 25.0%
!!! AAGGTTAAAGCATTTTACAA+TGG - chr5.1:16853780-16853799 MS.gene72784:CDS 25.0%
ATAACTGGAACGGAAAAATA+TGG + chr5.1:16853867-16853886 None:intergenic 30.0%
! TGCAACATCTTTTCTTTACA+TGG - chr5.1:16853752-16853771 MS.gene72784:CDS 30.0%
! GAAAAATATGGTTCTGCCAT+TGG + chr5.1:16853855-16853874 None:intergenic 35.0%
! GCATTTTACAATGGTTTCCT+TGG - chr5.1:16853789-16853808 MS.gene72784:CDS 35.0%
!! CTTTGAAGTTGCTTTCTTCA+GGG + chr5.1:16853635-16853654 None:intergenic 35.0%
!! TCTTTGAAGTTGCTTTCTTC+AGG + chr5.1:16853636-16853655 None:intergenic 35.0%
AAGGGGTCATCACATGAAAA+AGG - chr5.1:16853666-16853685 MS.gene72784:CDS 40.0%
ATGCATTGTGCAGCTTCTTA+TGG - chr5.1:16853534-16853553 MS.gene72784:CDS 40.0%
ATTCACTAGCAACATCTCCA+AGG + chr5.1:16853809-16853828 None:intergenic 40.0%
CTGCACAATGCATTAGTGTT+TGG + chr5.1:16853527-16853546 None:intergenic 40.0%
GAGATGTTGCTAGTGAATCA+AGG - chr5.1:16853811-16853830 MS.gene72784:CDS 40.0%
GCTAGTGAATCAAGGATGAT+GGG - chr5.1:16853819-16853838 MS.gene72784:CDS 40.0%
TGCTAGTGAATCAAGGATGA+TGG - chr5.1:16853818-16853837 MS.gene72784:CDS 40.0%
AGCACAAAAGCTTCACAGGT+TGG - chr5.1:16853716-16853735 MS.gene72784:CDS 45.0%
CTCAAGCACAAAAGCTTCAC+AGG - chr5.1:16853712-16853731 MS.gene72784:CDS 45.0%
GATGGGAATTGATGCACCAA+TGG - chr5.1:16853836-16853855 MS.gene72784:CDS 45.0%
GCACAAAAGCTTCACAGGTT+GGG - chr5.1:16853717-16853736 MS.gene72784:CDS 45.0%
GCAGCTTCTTATGGTGAAAG+AGG - chr5.1:16853543-16853562 MS.gene72784:CDS 45.0%
CCATCTCGTCGCAAAAGCTA+TGG - chr5.1:16853579-16853598 MS.gene72784:CDS 50.0%
! CCATAGCTTTTGCGACGAGA+TGG + chr5.1:16853582-16853601 None:intergenic 50.0%
! CTTTTGCGACGAGATGGTGT+TGG + chr5.1:16853576-16853595 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 16853492 16853896 16853492 ID=MS.gene72784
chr5.1 mRNA 16853492 16853896 16853492 ID=MS.gene72784.t1;Parent=MS.gene72784
chr5.1 exon 16853492 16853896 16853492 ID=MS.gene72784.t1.exon1;Parent=MS.gene72784.t1
chr5.1 CDS 16853492 16853896 16853492 ID=cds.MS.gene72784.t1;Parent=MS.gene72784.t1
Gene Sequence

>MS.gene72784

ATGGCTTCACAACTTGTTAGTGATGTCAAATGCCAAACACTAATGCATTGTGCAGCTTCTTATGGTGAAAGAGGAAGCAAACCAACACCATCTCGTCGCAAAAGCTATGGCTACTGTCGAGTAGAGAATAGCATCGTCTACCCTGAAGAAAGCAACTTCAAAGAACAAAAAAACAAGGGGTCATCACATGAAAAAGGTGTAAAACGTAACACAAATCTAGCTCAAGCACAAAAGCTTCACAGGTTGGGATTCTCTTACATTGCAACATCTTTTCTTTACATGGTAATTAAGGTTAAAGCATTTTACAATGGTTTCCTTGGAGATGTTGCTAGTGAATCAAGGATGATGGGAATTGATGCACCAATGGCAGAACCATATTTTTCCGTTCCAGTTATTCCAAATTAA

Protein sequence

>MS.gene72784.t1

MASQLVSDVKCQTLMHCAASYGERGSKPTPSRRKSYGYCRVENSIVYPEESNFKEQKNKGSSHEKGVKRNTNLAQAQKLHRLGFSYIATSFLYMVIKVKAFYNGFLGDVASESRMMGIDAPMAEPYFSVPVIPN