AlfalfaGEDB | MS.gene82146

M. sativa cultivar XinJiangDaYe / MS.gene82146

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82146.t1	XP_003612548.1	93.3	135	8	1	1	134	1	135	2.40E-62	248.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82146.t1	G7K5F6	93.3	135	8	1	1	134	1	135	1.7e-62	248.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene82146	0.862512	4.81E-64	-1.69E-46
MS.gene049217	MS.gene82146	0.868717	5.31E-66	-1.69E-46
MS.gene049222	MS.gene82146	0.8724	3.28E-67	-1.69E-46
MS.gene049232	MS.gene82146	0.875614	2.68E-68	-1.69E-46
MS.gene049285	MS.gene82146	0.907827	3.32E-81	-1.69E-46
MS.gene049320	MS.gene82146	0.90136	2.90E-78	-1.69E-46
MS.gene049609	MS.gene82146	0.852448	4.59E-61	-1.69E-46
MS.gene049627	MS.gene82146	0.810138	1.34E-50	-1.69E-46
MS.gene049633	MS.gene82146	0.806234	9.08E-50	-1.69E-46
MS.gene049805	MS.gene82146	0.835737	1.41E-56	-1.69E-46
MS.gene05010	MS.gene82146	0.873474	1.43E-67	-1.69E-46
MS.gene050163	MS.gene82146	0.856456	3.18E-62	-1.69E-46
MS.gene050165	MS.gene82146	0.845999	2.86E-59	-1.69E-46
MS.gene050170	MS.gene82146	0.817258	3.62E-52	-1.69E-46
MS.gene050216	MS.gene82146	0.828703	7.76E-55	-1.69E-46
MS.gene050323	MS.gene82146	0.846397	2.23E-59	-1.69E-46
MS.gene050325	MS.gene82146	-0.808859	2.52E-50	-1.69E-46
MS.gene050327	MS.gene82146	-0.80375	3.00E-49	-1.69E-46
MS.gene050333	MS.gene82146	0.84766	1.00E-59	-1.69E-46
MS.gene050381	MS.gene82146	-0.820985	5.13E-53	-1.69E-46
MS.gene050496	MS.gene82146	0.840442	8.67E-58	-1.69E-46
MS.gene050504	MS.gene82146	0.887223	1.72E-72	-1.69E-46
MS.gene050518	MS.gene82146	0.869582	2.78E-66	-1.69E-46
MS.gene050577	MS.gene82146	0.857116	2.03E-62	-1.69E-46
MS.gene050593	MS.gene82146	0.821425	4.06E-53	-1.69E-46
MS.gene050612	MS.gene82146	0.857944	1.16E-62	-1.69E-46
MS.gene050662	MS.gene82146	0.806742	7.10E-50	-1.69E-46
MS.gene050675	MS.gene82146	0.85054	1.59E-60	-1.69E-46
MS.gene050686	MS.gene82146	0.803862	2.85E-49	-1.69E-46
MS.gene050699	MS.gene82146	0.877419	6.38E-69	-1.69E-46
MS.gene050747	MS.gene82146	0.852614	4.11E-61	-1.69E-46
MS.gene050772	MS.gene82146	0.878155	3.53E-69	-1.69E-46
MS.gene05080	MS.gene82146	0.810468	1.14E-50	-1.69E-46
MS.gene050868	MS.gene82146	0.837502	5.01E-57	-1.69E-46
MS.gene050871	MS.gene82146	0.836423	9.44E-57	-1.69E-46
MS.gene050883	MS.gene82146	-0.82686	2.15E-54	-1.69E-46
MS.gene050950	MS.gene82146	0.847813	9.12E-60	-1.69E-46
MS.gene050986	MS.gene82146	0.863226	2.89E-64	-1.69E-46
MS.gene05100	MS.gene82146	0.862228	5.88E-64	-1.69E-46
MS.gene051127	MS.gene82146	-0.802316	5.95E-49	-1.69E-46
MS.gene051167	MS.gene82146	-0.807077	6.03E-50	-1.69E-46
MS.gene051364	MS.gene82146	-0.800176	1.63E-48	-1.69E-46
MS.gene051551	MS.gene82146	0.883237	5.32E-71	-1.69E-46
MS.gene051666	MS.gene82146	0.819886	9.18E-53	-1.69E-46
MS.gene051694	MS.gene82146	0.838182	3.35E-57	-1.69E-46
MS.gene051810	MS.gene82146	0.834663	2.63E-56	-1.69E-46
MS.gene051858	MS.gene82146	0.841943	3.50E-58	-1.69E-46
MS.gene052164	MS.gene82146	0.846152	2.60E-59	-1.69E-46
MS.gene052271	MS.gene82146	0.847239	1.31E-59	-1.69E-46
MS.gene052482	MS.gene82146	0.864862	8.96E-65	-1.69E-46
MS.gene052486	MS.gene82146	0.865225	6.89E-65	-1.69E-46
MS.gene052501	MS.gene82146	0.805704	1.17E-49	-1.69E-46
MS.gene052503	MS.gene82146	0.871097	8.86E-67	-1.69E-46
MS.gene052504	MS.gene82146	0.800656	1.30E-48	-1.69E-46
MS.gene052506	MS.gene82146	0.854376	1.28E-61	-1.69E-46
MS.gene052615	MS.gene82146	0.846387	2.24E-59	-1.69E-46
MS.gene052822	MS.gene82146	0.858483	7.99E-63	-1.69E-46
MS.gene052823	MS.gene82146	0.867588	1.23E-65	-1.69E-46
MS.gene052825	MS.gene82146	0.860604	1.84E-63	-1.69E-46
MS.gene052916	MS.gene82146	0.855403	6.46E-62	-1.69E-46
MS.gene052966	MS.gene82146	0.809619	1.73E-50	-1.69E-46
MS.gene053018	MS.gene82146	0.827204	1.78E-54	-1.69E-46
MS.gene053203	MS.gene82146	0.839263	1.76E-57	-1.69E-46
MS.gene053207	MS.gene82146	0.804774	1.84E-49	-1.69E-46
MS.gene053213	MS.gene82146	0.904944	7.23E-80	-1.69E-46
MS.gene053286	MS.gene82146	0.903121	4.81E-79	-1.69E-46
MS.gene053322	MS.gene82146	0.819719	1.00E-52	-1.69E-46
MS.gene053420	MS.gene82146	-0.821846	3.25E-53	-1.69E-46
MS.gene053436	MS.gene82146	0.877061	8.50E-69	-1.69E-46
MS.gene053480	MS.gene82146	0.821426	4.06E-53	-1.69E-46
MS.gene053549	MS.gene82146	-0.808734	2.68E-50	-1.69E-46
MS.gene053574	MS.gene82146	0.888565	5.28E-73	-1.69E-46
MS.gene053626	MS.gene82146	0.837094	6.37E-57	-1.69E-46
MS.gene053632	MS.gene82146	0.851406	9.06E-61	-1.69E-46
MS.gene053824	MS.gene82146	0.838216	3.28E-57	-1.69E-46
MS.gene053916	MS.gene82146	0.81727	3.60E-52	-1.69E-46
MS.gene054003	MS.gene82146	0.836117	1.13E-56	-1.69E-46
MS.gene054408	MS.gene82146	0.827961	1.17E-54	-1.69E-46
MS.gene054595	MS.gene82146	0.857561	1.50E-62	-1.69E-46
MS.gene054600	MS.gene82146	0.837558	4.84E-57	-1.69E-46
MS.gene054642	MS.gene82146	0.894238	2.98E-75	-1.69E-46
MS.gene054706	MS.gene82146	-0.800881	1.17E-48	-1.69E-46
MS.gene05470	MS.gene82146	0.832862	7.42E-56	-1.69E-46
MS.gene054927	MS.gene82146	0.814828	1.26E-51	-1.69E-46
MS.gene054954	MS.gene82146	0.903263	4.16E-79	-1.69E-46
MS.gene05505	MS.gene82146	0.827451	1.55E-54	-1.69E-46
MS.gene055154	MS.gene82146	-0.838785	2.34E-57	-1.69E-46
MS.gene055206	MS.gene82146	0.802405	5.70E-49	-1.69E-46
MS.gene055212	MS.gene82146	0.816121	6.51E-52	-1.69E-46
MS.gene055289	MS.gene82146	0.80562	1.22E-49	-1.69E-46
MS.gene055362	MS.gene82146	0.897705	1.09E-76	-1.69E-46
MS.gene055502	MS.gene82146	0.894131	3.29E-75	-1.69E-46
MS.gene055577	MS.gene82146	0.852516	4.39E-61	-1.69E-46
MS.gene055638	MS.gene82146	0.807287	5.44E-50	-1.69E-46
MS.gene055647	MS.gene82146	0.827319	1.67E-54	-1.69E-46
MS.gene055722	MS.gene82146	0.847903	8.62E-60	-1.69E-46
MS.gene055748	MS.gene82146	0.800901	1.16E-48	-1.69E-46
MS.gene055750	MS.gene82146	0.822978	1.77E-53	-1.69E-46
MS.gene055751	MS.gene82146	0.805527	1.28E-49	-1.69E-46
MS.gene055776	MS.gene82146	0.81315	2.96E-51	-1.69E-46
MS.gene05589	MS.gene82146	0.823304	1.49E-53	-1.69E-46
MS.gene05592	MS.gene82146	0.804388	2.21E-49	-1.69E-46
MS.gene055941	MS.gene82146	0.848895	4.58E-60	-1.69E-46
MS.gene056046	MS.gene82146	0.846281	2.40E-59	-1.69E-46
MS.gene056083	MS.gene82146	0.817548	3.11E-52	-1.69E-46
MS.gene056311	MS.gene82146	-0.806012	1.01E-49	-1.69E-46
MS.gene056374	MS.gene82146	0.881042	3.33E-70	-1.69E-46
MS.gene056409	MS.gene82146	0.823824	1.13E-53	-1.69E-46
MS.gene056448	MS.gene82146	0.822236	2.64E-53	-1.69E-46
MS.gene056504	MS.gene82146	0.879623	1.07E-69	-1.69E-46
MS.gene056510	MS.gene82146	0.865873	4.30E-65	-1.69E-46
MS.gene056549	MS.gene82146	0.800199	1.61E-48	-1.69E-46
MS.gene056610	MS.gene82146	0.819798	9.61E-53	-1.69E-46
MS.gene056657	MS.gene82146	0.910351	2.06E-82	-1.69E-46
MS.gene056692	MS.gene82146	0.826954	2.04E-54	-1.69E-46
MS.gene056878	MS.gene82146	0.819976	8.75E-53	-1.69E-46
MS.gene056942	MS.gene82146	0.800781	1.23E-48	-1.69E-46
MS.gene056946	MS.gene82146	0.836266	1.03E-56	-1.69E-46
MS.gene057014	MS.gene82146	-0.811201	7.88E-51	-1.69E-46
MS.gene057076	MS.gene82146	0.818857	1.57E-52	-1.69E-46
MS.gene057086	MS.gene82146	0.800144	1.65E-48	-1.69E-46
MS.gene057129	MS.gene82146	0.801005	1.10E-48	-1.69E-46
MS.gene057331	MS.gene82146	0.806115	9.62E-50	-1.69E-46
MS.gene057416	MS.gene82146	0.865717	4.82E-65	-1.69E-46
MS.gene057658	MS.gene82146	0.801945	7.09E-49	-1.69E-46
MS.gene057831	MS.gene82146	0.835425	1.69E-56	-1.69E-46
MS.gene05787	MS.gene82146	0.804161	2.47E-49	-1.69E-46
MS.gene057926	MS.gene82146	0.812926	3.32E-51	-1.69E-46
MS.gene058019	MS.gene82146	0.836627	8.38E-57	-1.69E-46
MS.gene058130	MS.gene82146	0.800021	1.75E-48	-1.69E-46
MS.gene058157	MS.gene82146	0.814828	1.26E-51	-1.69E-46
MS.gene058183	MS.gene82146	0.815125	1.09E-51	-1.69E-46
MS.gene058338	MS.gene82146	0.814043	1.88E-51	-1.69E-46
MS.gene05837	MS.gene82146	0.837472	5.10E-57	-1.69E-46
MS.gene058419	MS.gene82146	0.903042	5.22E-79	-1.69E-46
MS.gene058544	MS.gene82146	0.858798	6.43E-63	-1.69E-46
MS.gene058723	MS.gene82146	0.815639	8.34E-52	-1.69E-46
MS.gene058773	MS.gene82146	0.846017	2.83E-59	-1.69E-46
MS.gene058838	MS.gene82146	0.82074	5.85E-53	-1.69E-46
MS.gene05883	MS.gene82146	0.817012	4.11E-52	-1.69E-46
MS.gene059022	MS.gene82146	0.89578	6.92E-76	-1.69E-46
MS.gene059023	MS.gene82146	0.849659	2.81E-60	-1.69E-46
MS.gene059029	MS.gene82146	0.800754	1.24E-48	-1.69E-46
MS.gene059034	MS.gene82146	0.816588	5.12E-52	-1.69E-46
MS.gene05913	MS.gene82146	0.835942	1.25E-56	-1.69E-46
MS.gene05934	MS.gene82146	0.818402	2.00E-52	-1.69E-46
MS.gene059367	MS.gene82146	0.843599	1.27E-58	-1.69E-46
MS.gene059536	MS.gene82146	0.816125	6.50E-52	-1.69E-46
MS.gene059677	MS.gene82146	0.885922	5.35E-72	-1.69E-46
MS.gene059718	MS.gene82146	0.885951	5.22E-72	-1.69E-46
MS.gene059756	MS.gene82146	0.849537	3.03E-60	-1.69E-46
MS.gene059801	MS.gene82146	0.824049	9.96E-54	-1.69E-46
MS.gene059837	MS.gene82146	0.811936	5.45E-51	-1.69E-46
MS.gene059865	MS.gene82146	0.862655	4.34E-64	-1.69E-46
MS.gene059902	MS.gene82146	0.840237	9.81E-58	-1.69E-46
MS.gene060482	MS.gene82146	0.894411	2.53E-75	-1.69E-46
MS.gene060595	MS.gene82146	-0.817229	3.68E-52	-1.69E-46
MS.gene060703	MS.gene82146	0.865223	6.90E-65	-1.69E-46
MS.gene060762	MS.gene82146	0.843821	1.11E-58	-1.69E-46
MS.gene060778	MS.gene82146	0.849196	3.78E-60	-1.69E-46
MS.gene06077	MS.gene82146	0.889544	2.20E-73	-1.69E-46
MS.gene060804	MS.gene82146	0.823597	1.27E-53	-1.69E-46
MS.gene060835	MS.gene82146	0.820384	7.06E-53	-1.69E-46
MS.gene06092	MS.gene82146	0.838012	3.70E-57	-1.69E-46
MS.gene060982	MS.gene82146	0.849913	2.38E-60	-1.69E-46
MS.gene061134	MS.gene82146	0.80619	9.28E-50	-1.69E-46
MS.gene061150	MS.gene82146	0.837924	3.90E-57	-1.69E-46
MS.gene061171	MS.gene82146	0.80491	1.72E-49	-1.69E-46
MS.gene061200	MS.gene82146	0.830745	2.47E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82146.t1	MTR_5g026290	93.333	135	8	1	1	134	1	135	6.61e-92	262

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATATTTGTACTTTGTTAATT+TGG	0.112303	5.2:+16981605	None:intergenic
CTTTGTTAATTTGGAATAAC+TGG	0.189219	5.2:+16981614	None:intergenic
TCTTTGAAGTTGCTTTCTTC+AGG	0.231770	5.2:+16981860	None:intergenic
ATGCATTGTGCAGCTTCTTA+TGG	0.341347	5.2:-16981959	MS.gene82146:CDS
AAGGGGTCATCACATGAAAA+AGG	0.380796	5.2:-16981827	MS.gene82146:CDS
CTGCACAATGCATTAGTGTT+TGG	0.388403	5.2:+16981969	None:intergenic
CTTTGAAGTTGCTTTCTTCA+GGG	0.422990	5.2:+16981861	None:intergenic
GCACAAAAGCTTCACAGGTT+GGG	0.439585	5.2:-16981776	MS.gene82146:CDS
CTCAAGCACAAAAGCTTCAC+AGG	0.495602	5.2:-16981781	MS.gene82146:CDS
GCAGCTTCTTATGGTGAAAG+AGG	0.521502	5.2:-16981950	MS.gene82146:CDS
AGCACAAAAGCTTCACAGGT+TGG	0.529568	5.2:-16981777	MS.gene82146:CDS
TGCTAGTGAATCAAGGATGA+TGG	0.543307	5.2:-16981675	MS.gene82146:CDS
CCATCTCGTCGCAAAAGCTA+TGG	0.544204	5.2:-16981914	MS.gene82146:CDS
TTAATTTGGAATAACTGGAA+CGG	0.567626	5.2:+16981619	None:intergenic
GAGATGTTGCTAGTGAATCA+AGG	0.609919	5.2:-16981682	MS.gene82146:CDS
GCTAGTGAATCAAGGATGAT+GGG	0.613297	5.2:-16981674	MS.gene82146:CDS
ATTCACTAGCAACATCTCCA+AGG	0.613391	5.2:+16981687	None:intergenic
GATGGGAATTGATGCACCAA+TGG	0.622941	5.2:-16981657	MS.gene82146:CDS
CGATGCTATTCTCTACTCGA+CGG	0.702175	5.2:+16981888	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAAAGAACAAAAAAACAA+GGG	-	chr5.2:16981775-16981794	MS.gene82146:CDS	20.0%
!!!	TTTTCTTTACATGGTAATTA+AGG	-	chr5.2:16981888-16981907	MS.gene82146:CDS	20.0%
!	CTTCAAAGAACAAAAAAACA+AGG	-	chr5.2:16981774-16981793	MS.gene82146:CDS	25.0%
!	TCAAAGAACAAAAAAACAAG+GGG	-	chr5.2:16981776-16981795	MS.gene82146:CDS	25.0%
!!	TTAATTTGGAATAACTGGAA+CGG	+	chr5.2:16982004-16982023	None:intergenic	25.0%
!!!	AAGGTTAAAGCATTTTACAA+TGG	-	chr5.2:16981907-16981926	MS.gene82146:CDS	25.0%
	ATAACTGGAACGGAAAAATA+TGG	+	chr5.2:16981994-16982013	None:intergenic	30.0%
!	TGCAACATCTTTTCTTTACA+TGG	-	chr5.2:16981879-16981898	MS.gene82146:CDS	30.0%
!	GAAAAATATGGTTCTGCCAT+TGG	+	chr5.2:16981982-16982001	None:intergenic	35.0%
!	GCATTTTACAATGGTTTCCT+TGG	-	chr5.2:16981916-16981935	MS.gene82146:CDS	35.0%
!!	CTTTGAAGTTGCTTTCTTCA+GGG	+	chr5.2:16981762-16981781	None:intergenic	35.0%
!!	TCTTTGAAGTTGCTTTCTTC+AGG	+	chr5.2:16981763-16981782	None:intergenic	35.0%
	AAGGGGTCATCACATGAAAA+AGG	-	chr5.2:16981793-16981812	MS.gene82146:CDS	40.0%
	ATGCATTGTGCAGCTTCTTA+TGG	-	chr5.2:16981661-16981680	MS.gene82146:CDS	40.0%
	ATTCACTAGCAACATCTCCA+AGG	+	chr5.2:16981936-16981955	None:intergenic	40.0%
	CTGCACAATGCATTAGTGTT+TGG	+	chr5.2:16981654-16981673	None:intergenic	40.0%
	GAGATGTTGCTAGTGAATCA+AGG	-	chr5.2:16981938-16981957	MS.gene82146:CDS	40.0%
	GCTAGTGAATCAAGGATGAT+GGG	-	chr5.2:16981946-16981965	MS.gene82146:CDS	40.0%
	TGCTAGTGAATCAAGGATGA+TGG	-	chr5.2:16981945-16981964	MS.gene82146:CDS	40.0%
	AGCACAAAAGCTTCACAGGT+TGG	-	chr5.2:16981843-16981862	MS.gene82146:CDS	45.0%
	CGATGCTATTCTCTACTCGA+CGG	+	chr5.2:16981735-16981754	None:intergenic	45.0%
	CTCAAGCACAAAAGCTTCAC+AGG	-	chr5.2:16981839-16981858	MS.gene82146:CDS	45.0%
	GATGGGAATTGATGCACCAA+TGG	-	chr5.2:16981963-16981982	MS.gene82146:CDS	45.0%
	GCACAAAAGCTTCACAGGTT+GGG	-	chr5.2:16981844-16981863	MS.gene82146:CDS	45.0%
	GCAGCTTCTTATGGTGAAAG+AGG	-	chr5.2:16981670-16981689	MS.gene82146:CDS	45.0%
	CCATCTCGTCGCAAAAGCTA+TGG	-	chr5.2:16981706-16981725	MS.gene82146:CDS	50.0%
!	CCATAGCTTTTGCGACGAGA+TGG	+	chr5.2:16981709-16981728	None:intergenic	50.0%
!	CTTTTGCGACGAGATGGTGT+TGG	+	chr5.2:16981703-16981722	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	16981619	16982023	16981619	ID=MS.gene82146
chr5.2	mRNA	16981619	16982023	16981619	ID=MS.gene82146.t1;Parent=MS.gene82146
chr5.2	exon	16981619	16982023	16981619	ID=MS.gene82146.t1.exon1;Parent=MS.gene82146.t1
chr5.2	CDS	16981619	16982023	16981619	ID=cds.MS.gene82146.t1;Parent=MS.gene82146.t1

Gene Sequence

>MS.gene82146

ATGGCTTCACAACTTGTTAGTGATGTCAAATGCCAAACACTAATGCATTGTGCAGCTTCTTATGGTGAAAGAGGAAGCAAACCAACACCATCTCGTCGCAAAAGCTATGGCTACCGTCGAGTAGAGAATAGCATCGTCTACCCTGAAGAAAGCAACTTCAAAGAACAAAAAAACAAGGGGTCATCACATGAAAAAGGTGTAAAACGTAACACAAATCTAGCTCAAGCACAAAAGCTTCACAGGTTGGGATTCTCTTACATTGCAACATCTTTTCTTTACATGGTAATTAAGGTTAAAGCATTTTACAATGGTTTCCTTGGAGATGTTGCTAGTGAATCAAGGATGATGGGAATTGATGCACCAATGGCAGAACCATATTTTTCCGTTCCAGTTATTCCAAATTAA

Protein sequence

>MS.gene82146.t1

MASQLVSDVKCQTLMHCAASYGERGSKPTPSRRKSYGYRRVENSIVYPEESNFKEQKNKGSSHEKGVKRNTNLAQAQKLHRLGFSYIATSFLYMVIKVKAFYNGFLGDVASESRMMGIDAPMAEPYFSVPVIPN