Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57486.t1 | XP_013444940.1 | 97.3 | 561 | 15 | 0 | 1 | 561 | 1 | 561 | 2.00E-259 | 904.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57486.t1 | A0A072TP55 | 97.3 | 561 | 15 | 0 | 1 | 561 | 24 | 584 | 1.4e-259 | 904.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049178 | MS.gene57486 | 0.816066 | 6.70E-52 | -1.69E-46 |
| MS.gene049435 | MS.gene57486 | 0.814347 | 1.61E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene57486 | 0.81822 | 2.20E-52 | -1.69E-46 |
| MS.gene050137 | MS.gene57486 | 0.817809 | 2.72E-52 | -1.69E-46 |
| MS.gene050156 | MS.gene57486 | 0.81906 | 1.42E-52 | -1.69E-46 |
| MS.gene050226 | MS.gene57486 | 0.808375 | 3.19E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene57486 | 0.817661 | 2.94E-52 | -1.69E-46 |
| MS.gene050228 | MS.gene57486 | 0.820025 | 8.53E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene57486 | 0.824926 | 6.19E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene57486 | 0.844575 | 6.95E-59 | -1.69E-46 |
| MS.gene050721 | MS.gene57486 | 0.840447 | 8.65E-58 | -1.69E-46 |
| MS.gene050724 | MS.gene57486 | 0.827388 | 1.61E-54 | -1.69E-46 |
| MS.gene050780 | MS.gene57486 | 0.827662 | 1.38E-54 | -1.69E-46 |
| MS.gene050787 | MS.gene57486 | 0.822804 | 1.95E-53 | -1.69E-46 |
| MS.gene050807 | MS.gene57486 | 0.80196 | 7.04E-49 | -1.69E-46 |
| MS.gene050959 | MS.gene57486 | 0.826825 | 2.19E-54 | -1.69E-46 |
| MS.gene051207 | MS.gene57486 | 0.805118 | 1.56E-49 | -1.69E-46 |
| MS.gene051247 | MS.gene57486 | 0.844277 | 8.35E-59 | -1.69E-46 |
| MS.gene051265 | MS.gene57486 | 0.843055 | 1.77E-58 | -1.69E-46 |
| MS.gene051700 | MS.gene57486 | 0.815148 | 1.07E-51 | -1.69E-46 |
| MS.gene051705 | MS.gene57486 | 0.81848 | 1.92E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene57486 | 0.81967 | 1.03E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene57486 | 0.812005 | 5.27E-51 | -1.69E-46 |
| MS.gene052020 | MS.gene57486 | 0.823199 | 1.58E-53 | -1.69E-46 |
| MS.gene052613 | MS.gene57486 | 0.812044 | 5.17E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene57486 | 0.819408 | 1.18E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene57486 | 0.8035 | 3.38E-49 | -1.69E-46 |
| MS.gene053054 | MS.gene57486 | -0.84516 | 4.83E-59 | -1.69E-46 |
| MS.gene053147 | MS.gene57486 | 0.804437 | 2.16E-49 | -1.69E-46 |
| MS.gene053221 | MS.gene57486 | 0.834603 | 2.72E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene57486 | 0.828502 | 8.68E-55 | -1.69E-46 |
| MS.gene053331 | MS.gene57486 | 0.804543 | 2.05E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene57486 | -0.820719 | 5.91E-53 | -1.69E-46 |
| MS.gene054467 | MS.gene57486 | 0.84857 | 5.64E-60 | -1.69E-46 |
| MS.gene054468 | MS.gene57486 | 0.849911 | 2.39E-60 | -1.69E-46 |
| MS.gene054576 | MS.gene57486 | 0.803155 | 3.99E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene57486 | 0.834804 | 2.43E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene57486 | 0.80986 | 1.54E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene57486 | 0.817657 | 2.94E-52 | -1.69E-46 |
| MS.gene055357 | MS.gene57486 | 0.823448 | 1.38E-53 | -1.69E-46 |
| MS.gene055358 | MS.gene57486 | 0.823355 | 1.45E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene57486 | 0.807065 | 6.06E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene57486 | 0.818425 | 1.97E-52 | -1.69E-46 |
| MS.gene055818 | MS.gene57486 | 0.810031 | 1.41E-50 | -1.69E-46 |
| MS.gene05604 | MS.gene57486 | 0.824241 | 8.98E-54 | -1.69E-46 |
| MS.gene056050 | MS.gene57486 | 0.809125 | 2.21E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene57486 | 0.820784 | 5.71E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene57486 | 0.822504 | 2.29E-53 | -1.69E-46 |
| MS.gene057414 | MS.gene57486 | 0.808304 | 3.31E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene57486 | 0.81638 | 5.70E-52 | -1.69E-46 |
| MS.gene058168 | MS.gene57486 | 0.824491 | 7.84E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene57486 | 0.829852 | 4.08E-55 | -1.69E-46 |
| MS.gene058636 | MS.gene57486 | 0.82624 | 3.02E-54 | -1.69E-46 |
| MS.gene058829 | MS.gene57486 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene059084 | MS.gene57486 | 0.811802 | 5.83E-51 | -1.69E-46 |
| MS.gene059625 | MS.gene57486 | 0.802413 | 5.68E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene57486 | 0.84472 | 6.35E-59 | -1.69E-46 |
| MS.gene060137 | MS.gene57486 | -0.817728 | 2.84E-52 | -1.69E-46 |
| MS.gene060637 | MS.gene57486 | 0.825211 | 5.31E-54 | -1.69E-46 |
| MS.gene060803 | MS.gene57486 | 0.815942 | 7.14E-52 | -1.69E-46 |
| MS.gene061040 | MS.gene57486 | -0.811681 | 6.20E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052565 | MS.gene57486 | PPI |
| MS.gene57486 | MS.gene33842 | PPI |
| MS.gene41659 | MS.gene57486 | PPI |
| MS.gene57486 | MS.gene06253 | PPI |
| MS.gene57486 | MS.gene052564 | PPI |
| MS.gene013180 | MS.gene57486 | PPI |
| MS.gene055531 | MS.gene57486 | PPI |
| MS.gene57486 | MS.gene28664 | PPI |
| MS.gene57486 | MS.gene32213 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57486.t1 | MTR_8g032570 | 96.970 | 561 | 17 | 0 | 1 | 561 | 24 | 584 | 0.0 | 1093 |
| MS.gene57486.t1 | MTR_8g032570 | 96.970 | 561 | 17 | 0 | 1 | 561 | 1 | 561 | 0.0 | 1093 |
| MS.gene57486.t1 | MTR_7g076220 | 77.837 | 564 | 118 | 3 | 1 | 561 | 1 | 560 | 0.0 | 879 |
| MS.gene57486.t1 | MTR_3g098730 | 35.382 | 537 | 321 | 13 | 37 | 560 | 30 | 553 | 2.12e-80 | 263 |
| MS.gene57486.t1 | MTR_4g091320 | 29.433 | 564 | 351 | 14 | 25 | 559 | 27 | 572 | 1.10e-62 | 216 |
| MS.gene57486.t1 | MTR_5g092900 | 53.279 | 122 | 18 | 4 | 372 | 491 | 32 | 116 | 1.42e-24 | 99.0 |
| MS.gene57486.t1 | MTR_1g018180 | 29.237 | 236 | 155 | 6 | 238 | 466 | 101 | 331 | 3.15e-19 | 89.7 |
| MS.gene57486.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 484 | 721 | 1.85e-17 | 86.7 |
| MS.gene57486.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 480 | 717 | 1.89e-17 | 86.3 |
| MS.gene57486.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 363 | 600 | 1.94e-17 | 86.3 |
| MS.gene57486.t1 | MTR_4g007850 | 32.237 | 152 | 100 | 3 | 409 | 557 | 364 | 515 | 7.17e-16 | 80.9 |
| MS.gene57486.t1 | MTR_7g097020 | 28.512 | 242 | 154 | 9 | 238 | 469 | 522 | 754 | 1.44e-15 | 80.5 |
| MS.gene57486.t1 | MTR_7g097020 | 31.034 | 203 | 134 | 4 | 337 | 536 | 496 | 695 | 4.23e-11 | 66.2 |
| MS.gene57486.t1 | MTR_3g103050 | 26.577 | 222 | 147 | 7 | 238 | 448 | 91 | 307 | 3.17e-15 | 77.8 |
| MS.gene57486.t1 | MTR_7g078330 | 27.240 | 279 | 194 | 7 | 201 | 474 | 370 | 644 | 1.49e-14 | 77.0 |
| MS.gene57486.t1 | MTR_7g078330 | 26.667 | 285 | 196 | 7 | 146 | 425 | 361 | 637 | 1.45e-12 | 70.9 |
| MS.gene57486.t1 | MTR_3g466220 | 29.949 | 197 | 123 | 5 | 180 | 365 | 566 | 758 | 4.30e-11 | 66.2 |
| MS.gene57486.t1 | MTR_3g466220 | 29.949 | 197 | 123 | 5 | 180 | 365 | 545 | 737 | 4.84e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57486.t1 | AT2G45720 | 67.201 | 561 | 176 | 4 | 1 | 561 | 1 | 553 | 0.0 | 755 |
| MS.gene57486.t1 | AT2G45720 | 67.201 | 561 | 176 | 4 | 1 | 561 | 1 | 553 | 0.0 | 755 |
| MS.gene57486.t1 | AT2G45720 | 67.201 | 561 | 176 | 4 | 1 | 561 | 1 | 553 | 0.0 | 755 |
| MS.gene57486.t1 | AT2G45720 | 67.201 | 561 | 176 | 4 | 1 | 561 | 1 | 553 | 0.0 | 755 |
| MS.gene57486.t1 | AT2G45720 | 67.201 | 561 | 176 | 4 | 1 | 561 | 1 | 553 | 0.0 | 755 |
| MS.gene57486.t1 | AT1G01830 | 63.214 | 560 | 200 | 4 | 3 | 560 | 19 | 574 | 0.0 | 675 |
| MS.gene57486.t1 | AT1G01830 | 63.214 | 560 | 200 | 4 | 3 | 560 | 19 | 574 | 0.0 | 675 |
| MS.gene57486.t1 | AT1G01830 | 63.214 | 560 | 200 | 4 | 3 | 560 | 19 | 574 | 0.0 | 675 |
| MS.gene57486.t1 | AT5G50900 | 37.736 | 530 | 316 | 8 | 40 | 560 | 30 | 554 | 4.63e-102 | 319 |
| MS.gene57486.t1 | AT1G61350 | 27.605 | 547 | 361 | 12 | 38 | 559 | 32 | 568 | 5.66e-60 | 209 |
| MS.gene57486.t1 | AT2G05810 | 32.222 | 540 | 340 | 12 | 37 | 558 | 41 | 572 | 2.01e-49 | 180 |
| MS.gene57486.t1 | AT2G05810 | 32.222 | 540 | 340 | 12 | 37 | 558 | 41 | 572 | 2.01e-49 | 180 |
| MS.gene57486.t1 | AT5G58680 | 27.869 | 244 | 164 | 6 | 234 | 470 | 99 | 337 | 9.77e-21 | 94.4 |
| MS.gene57486.t1 | AT3G01400 | 25.862 | 232 | 160 | 6 | 238 | 462 | 105 | 331 | 7.71e-16 | 79.7 |
| MS.gene57486.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 540 | 818 | 7.47e-15 | 78.6 |
| MS.gene57486.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 537 | 815 | 7.52e-15 | 78.6 |
| MS.gene57486.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 537 | 815 | 7.52e-15 | 78.6 |
| MS.gene57486.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 348 | 626 | 3.36e-14 | 75.9 |
| MS.gene57486.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 348 | 626 | 3.36e-14 | 75.9 |
| MS.gene57486.t1 | AT2G28830 | 25.697 | 323 | 184 | 7 | 151 | 464 | 280 | 555 | 1.60e-12 | 70.5 |
| MS.gene57486.t1 | AT5G67340 | 27.875 | 287 | 192 | 10 | 146 | 425 | 415 | 693 | 5.25e-12 | 69.3 |
| MS.gene57486.t1 | AT5G67340 | 27.875 | 287 | 192 | 10 | 146 | 425 | 415 | 693 | 5.25e-12 | 69.3 |
| MS.gene57486.t1 | AT3G20170 | 26.115 | 314 | 222 | 7 | 185 | 493 | 94 | 402 | 9.37e-12 | 67.8 |
Find 87 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATGATCATTTCAAAAT+TGG | 0.161848 | 8.3:+61195668 | MS.gene57486:CDS |
| AGTGATCTTGATGCATTAAT+TGG | 0.208436 | 8.3:+61195855 | MS.gene57486:CDS |
| TAAGCTTGATTTGAATTTAA+AGG | 0.223787 | 8.3:+61195878 | MS.gene57486:CDS |
| GATAAATCTGATAGCTTTGA+TGG | 0.243067 | 8.3:-61195699 | None:intergenic |
| AGGCGGCGTTCGAAGCATTT+TGG | 0.264072 | 8.3:+61196622 | MS.gene57486:CDS |
| TGATAAAAGTGTGCCGAATT+TGG | 0.271000 | 8.3:+61196982 | MS.gene57486:CDS |
| CTTAACAATGGAATGTTGTT+AGG | 0.279304 | 8.3:+61196521 | MS.gene57486:CDS |
| ACAAAAGCAAGAGAGGTAAA+AGG | 0.285811 | 8.3:+61195630 | MS.gene57486:CDS |
| ATGATTTCATATGGAGCAAT+AGG | 0.295280 | 8.3:+61197100 | MS.gene57486:CDS |
| TTGTACTTTGAAGAATATTT+CGG | 0.300330 | 8.3:+61196436 | MS.gene57486:CDS |
| TCCGGAAGTGAGACAGGTTT+TGG | 0.307249 | 8.3:+61196463 | MS.gene57486:CDS |
| CTTATAAGGCTTGTTGAATC+AGG | 0.333515 | 8.3:+61196263 | MS.gene57486:CDS |
| TTCAAAGAATGCACTTTGTA+AGG | 0.336893 | 8.3:+61195737 | MS.gene57486:CDS |
| GAAGAATATTTCGGCTGTTC+CGG | 0.352070 | 8.3:+61196445 | MS.gene57486:CDS |
| AAATACGCAGTTTGTTGCCT+TGG | 0.353804 | 8.3:+61197043 | MS.gene57486:CDS |
| CAGGAGCAATGTTGTTGCTT+TGG | 0.355138 | 8.3:+61196118 | MS.gene57486:CDS |
| TGAAACACATTGCAATATAA+AGG | 0.363878 | 8.3:+61195986 | MS.gene57486:CDS |
| GAGGAAACTCTGGTTTCTCT+TGG | 0.367522 | 8.3:+61196716 | MS.gene57486:CDS |
| TCCGTTAGTCGAGCTTTGTC+AGG | 0.385091 | 8.3:+61196382 | MS.gene57486:CDS |
| ATTCCAAATGACCGATTTGA+AGG | 0.386702 | 8.3:-61196022 | None:intergenic |
| AGAAGATGAGAAGAATGTTT+TGG | 0.395990 | 8.3:+61196085 | MS.gene57486:CDS |
| ACTTTGTAAGGAACAATTGC+AGG | 0.398251 | 8.3:+61195749 | MS.gene57486:CDS |
| GCCAAAACCTGTCTCACTTC+CGG | 0.399317 | 8.3:-61196464 | None:intergenic |
| ACGCCTTCAAATCGGTCATT+TGG | 0.403237 | 8.3:+61196019 | MS.gene57486:CDS |
| ATTAGCTGAGTTATGTTTGA+AGG | 0.406726 | 8.3:+61195803 | MS.gene57486:CDS |
| GGAATCGAAACACAAAGCTT+TGG | 0.406964 | 8.3:+61196040 | MS.gene57486:CDS |
| CCGGTGGTAGCTGCGAAAAC+TGG | 0.410752 | 8.3:+61196213 | MS.gene57486:CDS |
| TCAAGCAATTGAACCAAATT+CGG | 0.411317 | 8.3:-61196995 | None:intergenic |
| ATTGAGATGAAGAAAATGTT+AGG | 0.426418 | 8.3:+61196830 | MS.gene57486:CDS |
| ATATGCTCTCTTGTTGAATC+CGG | 0.443973 | 8.3:+61196194 | MS.gene57486:CDS |
| CTTATTGAGATGGAGATTCC+AGG | 0.448410 | 8.3:+61197136 | MS.gene57486:CDS |
| CAAAAGCAAGAGAGGTAAAA+GGG | 0.451511 | 8.3:+61195631 | MS.gene57486:CDS |
| CAGCAACATCAACAAGGATA+AGG | 0.452305 | 8.3:+61196153 | MS.gene57486:CDS |
| AAGAAAATGTTAGGTGAAGT+TGG | 0.452479 | 8.3:+61196839 | MS.gene57486:CDS |
| AGGCTATTGTTTCTCTTCAA+AGG | 0.458318 | 8.3:+61196306 | MS.gene57486:CDS |
| AGGTAAAAGGGTTTCAAGGT+AGG | 0.459525 | 8.3:+61195643 | MS.gene57486:CDS |
| AGCAACATCAACAAGGATAA+GGG | 0.463755 | 8.3:+61196154 | MS.gene57486:CDS |
| GGCTGTTCCGGAAGTGAGAC+AGG | 0.464868 | 8.3:+61196457 | MS.gene57486:CDS |
| GGCTGAAGAAGGCGTTGTTA+AGG | 0.466264 | 8.3:+61196484 | MS.gene57486:CDS |
| TGCTTGAAAGATTGGAAAGA+GGG | 0.471530 | 8.3:+61197170 | MS.gene57486:CDS |
| TTACTCGCACGCCTTCAAAT+CGG | 0.478346 | 8.3:+61196011 | MS.gene57486:CDS |
| AAGAGGTTGATGATTTCATA+TGG | 0.478705 | 8.3:+61197091 | MS.gene57486:CDS |
| AGAGAGGTAAAAGGGTTTCA+AGG | 0.482668 | 8.3:+61195639 | MS.gene57486:CDS |
| CTATTGATCAACATGCTTGA+GGG | 0.487366 | 8.3:+61196872 | MS.gene57486:CDS |
| TGATTTCATATGGAGCAATA+GGG | 0.489291 | 8.3:+61197101 | MS.gene57486:CDS |
| TCAAGCATGTTGATCAATAG+AGG | 0.491275 | 8.3:-61196869 | None:intergenic |
| TTCAACAGAAGAATGGTTGT+TGG | 0.494650 | 8.3:+61195578 | MS.gene57486:CDS |
| TAGTGCTATTGCTAAAGAAA+AGG | 0.499049 | 8.3:+61196286 | MS.gene57486:CDS |
| ATCGGTTTCCGAGGAAACTC+TGG | 0.509299 | 8.3:+61196706 | MS.gene57486:CDS |
| ATACATGTTCAACAGAAGAA+TGG | 0.512048 | 8.3:+61195571 | MS.gene57486:CDS |
| TCTATTGATCAACATGCTTG+AGG | 0.513990 | 8.3:+61196871 | MS.gene57486:CDS |
| ACAGCAGATTCTTGAGGAAG+TGG | 0.514857 | 8.3:-61196659 | None:intergenic |
| CTTCCTCAAGAATCTGCTGT+TGG | 0.535097 | 8.3:+61196662 | MS.gene57486:CDS |
| TTGCTTGAAAGATTGGAAAG+AGG | 0.535251 | 8.3:+61197169 | MS.gene57486:CDS |
| GACACAACTCGTGCAATCGT+TGG | 0.538831 | 8.3:+61196344 | MS.gene57486:CDS |
| TGCGAAACAATGCGCTGCAT+CGG | 0.539824 | 8.3:+61196784 | MS.gene57486:CDS |
| ACCTGACAAAGCTCGACTAA+CGG | 0.545060 | 8.3:-61196383 | None:intergenic |
| TCTTCAAAGGTTGTCTATGA+CGG | 0.545473 | 8.3:+61196319 | MS.gene57486:CDS |
| TCTCGTTGCATCGGTTTCCG+AGG | 0.548267 | 8.3:+61196697 | MS.gene57486:CDS |
| GCGCTGCATCGGTTATATGC+AGG | 0.549822 | 8.3:+61196795 | MS.gene57486:CDS |
| TCTGAAGAAGCTTATTGAGA+TGG | 0.553377 | 8.3:+61197126 | MS.gene57486:CDS |
| ATTGAGGAATCTCGTTGCAT+CGG | 0.553997 | 8.3:+61196688 | MS.gene57486:CDS |
| ATGATCAATCTTCTTAACAA+TGG | 0.556218 | 8.3:+61196509 | MS.gene57486:CDS |
| AAGAGAAACCAGAGTTTCCT+CGG | 0.556662 | 8.3:-61196714 | None:intergenic |
| TTAGTCGAGCTTTGTCAGGT+TGG | 0.558482 | 8.3:+61196386 | MS.gene57486:CDS |
| TGCTCTTACAAAAGCAAGAG+AGG | 0.561490 | 8.3:+61195623 | MS.gene57486:CDS |
| TGCAATCGTTGGACATGGAG+GGG | 0.562940 | 8.3:+61196355 | MS.gene57486:CDS |
| GAAAACTGGTTAGTTTCTGA+AGG | 0.563650 | 8.3:+61196227 | MS.gene57486:CDS |
| GTGCAATCGTTGGACATGGA+GGG | 0.565053 | 8.3:+61196354 | MS.gene57486:CDS |
| TGCTCTCTTGTTGAATCCGG+TGG | 0.570371 | 8.3:+61196197 | MS.gene57486:CDS |
| AGAAGGTCTGTTATATCAGA+AGG | 0.575080 | 8.3:+61196602 | MS.gene57486:CDS |
| CTTCTTGTAAGAAATGCAAG+AGG | 0.577874 | 8.3:+61197074 | MS.gene57486:CDS |
| TAAAAGGGTTTCAAGGTAGG+TGG | 0.577886 | 8.3:+61195646 | MS.gene57486:CDS |
| AAATCGAAGAGAGGTTAAGA+AGG | 0.584354 | 8.3:+61196958 | MS.gene57486:CDS |
| ACTCGTGCAATCGTTGGACA+TGG | 0.608350 | 8.3:+61196350 | MS.gene57486:CDS |
| GATTCAACAAGCCTTATAAG+TGG | 0.616500 | 8.3:-61196260 | None:intergenic |
| TACAAGAAGAAAGTGATCCA+AGG | 0.619451 | 8.3:-61197060 | None:intergenic |
| TACTATACTATCTTCCACCA+TGG | 0.626237 | 8.3:-61195535 | None:intergenic |
| TCAACAAGCCTTATAAGTGG+TGG | 0.632485 | 8.3:-61196257 | None:intergenic |
| CAAGCAATGAGAATCTGAGA+AGG | 0.635445 | 8.3:+61196585 | MS.gene57486:CDS |
| CGTGCAATCGTTGGACATGG+AGG | 0.635524 | 8.3:+61196353 | MS.gene57486:CDS |
| TTCAAAACATGAACTAAACA+AGG | 0.642239 | 8.3:-61196746 | None:intergenic |
| GCACCAACAGCAGATTCTTG+AGG | 0.646827 | 8.3:-61196665 | None:intergenic |
| AATCTGCTGTTGGTGCATTG+AGG | 0.654433 | 8.3:+61196672 | MS.gene57486:CDS |
| TGCTTACAGCAACATCAACA+AGG | 0.669898 | 8.3:+61196147 | MS.gene57486:CDS |
| TCTGTCGCAAAATCGAAGAG+AGG | 0.676427 | 8.3:+61196949 | MS.gene57486:CDS |
| AGGTCTGTTATATCAGAAGG+CGG | 0.697553 | 8.3:+61196605 | MS.gene57486:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAATGATCATTTCAAAAT+TGG | + | chr8.3:61195668-61195687 | MS.gene57486:CDS | 20.0% |
| !!! | TAAGCTTGATTTGAATTTAA+AGG | + | chr8.3:61195878-61195897 | MS.gene57486:CDS | 20.0% |
| !!! | TTGTACTTTGAAGAATATTT+CGG | + | chr8.3:61196436-61196455 | MS.gene57486:CDS | 20.0% |
| ! | ATGATCAATCTTCTTAACAA+TGG | + | chr8.3:61196509-61196528 | MS.gene57486:CDS | 25.0% |
| ! | ATTGAGATGAAGAAAATGTT+AGG | + | chr8.3:61196830-61196849 | MS.gene57486:CDS | 25.0% |
| ! | TAAAAAGTTGCTTGAAAGAT+TGG | + | chr8.3:61197162-61197181 | MS.gene57486:CDS | 25.0% |
| ! | TGAAACACATTGCAATATAA+AGG | + | chr8.3:61195986-61196005 | MS.gene57486:CDS | 25.0% |
| ! | TGTTTGAAGGAAAAATATGA+AGG | + | chr8.3:61195816-61195835 | MS.gene57486:CDS | 25.0% |
| ! | TTCAAAACATGAACTAAACA+AGG | - | chr8.3:61196749-61196768 | None:intergenic | 25.0% |
| !!! | TTAGTTCATGTTTTGAAATC+AGG | + | chr8.3:61196752-61196771 | MS.gene57486:CDS | 25.0% |
| AAGAAAATGTTAGGTGAAGT+TGG | + | chr8.3:61196839-61196858 | MS.gene57486:CDS | 30.0% | |
| AGTGATCTTGATGCATTAAT+TGG | + | chr8.3:61195855-61195874 | MS.gene57486:CDS | 30.0% | |
| ATACATGTTCAACAGAAGAA+TGG | + | chr8.3:61195571-61195590 | MS.gene57486:CDS | 30.0% | |
| ATGATTTCATATGGAGCAAT+AGG | + | chr8.3:61197100-61197119 | MS.gene57486:CDS | 30.0% | |
| ATTAGCTGAGTTATGTTTGA+AGG | + | chr8.3:61195803-61195822 | MS.gene57486:CDS | 30.0% | |
| CTTAACAATGGAATGTTGTT+AGG | + | chr8.3:61196521-61196540 | MS.gene57486:CDS | 30.0% | |
| TCAAGCAATTGAACCAAATT+CGG | - | chr8.3:61196998-61197017 | None:intergenic | 30.0% | |
| TGATTTCATATGGAGCAATA+GGG | + | chr8.3:61197101-61197120 | MS.gene57486:CDS | 30.0% | |
| TTAACAAAAACTGGTGTACT+TGG | + | chr8.3:61195912-61195931 | MS.gene57486:CDS | 30.0% | |
| TTCAAAGAATGCACTTTGTA+AGG | + | chr8.3:61195737-61195756 | MS.gene57486:CDS | 30.0% | |
| ! | AGAAGATGAGAAGAATGTTT+TGG | + | chr8.3:61196085-61196104 | MS.gene57486:CDS | 30.0% |
| ! | AGTGCATTCTTTGAAAAACA+AGG | - | chr8.3:61195732-61195751 | None:intergenic | 30.0% |
| ! | GTGCATTCTTTGAAAAACAA+GGG | - | chr8.3:61195731-61195750 | None:intergenic | 30.0% |
| ! | TAGTGCTATTGCTAAAGAAA+AGG | + | chr8.3:61196286-61196305 | MS.gene57486:CDS | 30.0% |
| !! | AAGAGGTTGATGATTTCATA+TGG | + | chr8.3:61197091-61197110 | MS.gene57486:CDS | 30.0% |
| !! | GATAAATCTGATAGCTTTGA+TGG | - | chr8.3:61195702-61195721 | None:intergenic | 30.0% |
| !! | TGTAGCTTGTTAACAAAAAC+TGG | + | chr8.3:61195903-61195922 | MS.gene57486:CDS | 30.0% |
| !!! | GTTTTGAAATCAGGATCATT+AGG | + | chr8.3:61196761-61196780 | MS.gene57486:CDS | 30.0% |
| !!! | TATTTTTTCGCAGTGTTTTG+TGG | - | chr8.3:61197028-61197047 | None:intergenic | 30.0% |
| AAATCGAAGAGAGGTTAAGA+AGG | + | chr8.3:61196958-61196977 | MS.gene57486:CDS | 35.0% | |
| ACAAAAGCAAGAGAGGTAAA+AGG | + | chr8.3:61195630-61195649 | MS.gene57486:CDS | 35.0% | |
| ACTTTGTAAGGAACAATTGC+AGG | + | chr8.3:61195749-61195768 | MS.gene57486:CDS | 35.0% | |
| AGAAGGTCTGTTATATCAGA+AGG | + | chr8.3:61196602-61196621 | MS.gene57486:CDS | 35.0% | |
| AGCAACATCAACAAGGATAA+GGG | + | chr8.3:61196154-61196173 | MS.gene57486:CDS | 35.0% | |
| ATATGCTCTCTTGTTGAATC+CGG | + | chr8.3:61196194-61196213 | MS.gene57486:CDS | 35.0% | |
| CAAAAGCAAGAGAGGTAAAA+GGG | + | chr8.3:61195631-61195650 | MS.gene57486:CDS | 35.0% | |
| CTATTGATCAACATGCTTGA+GGG | + | chr8.3:61196872-61196891 | MS.gene57486:CDS | 35.0% | |
| CTTCTTGTAAGAAATGCAAG+AGG | + | chr8.3:61197074-61197093 | MS.gene57486:CDS | 35.0% | |
| TACAAGAAGAAAGTGATCCA+AGG | - | chr8.3:61197063-61197082 | None:intergenic | 35.0% | |
| TCAAGCATGTTGATCAATAG+AGG | - | chr8.3:61196872-61196891 | None:intergenic | 35.0% | |
| TCTATTGATCAACATGCTTG+AGG | + | chr8.3:61196871-61196890 | MS.gene57486:CDS | 35.0% | |
| TCTGAAGAAGCTTATTGAGA+TGG | + | chr8.3:61197126-61197145 | MS.gene57486:CDS | 35.0% | |
| TCTTCAAAGGTTGTCTATGA+CGG | + | chr8.3:61196319-61196338 | MS.gene57486:CDS | 35.0% | |
| TGCTTGAAAGATTGGAAAGA+GGG | + | chr8.3:61197170-61197189 | MS.gene57486:CDS | 35.0% | |
| TTCAACAGAAGAATGGTTGT+TGG | + | chr8.3:61195578-61195597 | MS.gene57486:CDS | 35.0% | |
| TTGCTTGAAAGATTGGAAAG+AGG | + | chr8.3:61197169-61197188 | MS.gene57486:CDS | 35.0% | |
| ! | AGGCTATTGTTTCTCTTCAA+AGG | + | chr8.3:61196306-61196325 | MS.gene57486:CDS | 35.0% |
| ! | CATTCTTTGAAAAACAAGGG+TGG | - | chr8.3:61195728-61195747 | None:intergenic | 35.0% |
| ! | GATTCAACAAGCCTTATAAG+TGG | - | chr8.3:61196263-61196282 | None:intergenic | 35.0% |
| ! | TGATAAAAGTGTGCCGAATT+TGG | + | chr8.3:61196982-61197001 | MS.gene57486:CDS | 35.0% |
| !! | ATTCCAAATGACCGATTTGA+AGG | - | chr8.3:61196025-61196044 | None:intergenic | 35.0% |
| !! | CTTATAAGGCTTGTTGAATC+AGG | + | chr8.3:61196263-61196282 | MS.gene57486:CDS | 35.0% |
| !! | CTTGCTTTTGTAAGAGCAAT+AGG | - | chr8.3:61195621-61195640 | None:intergenic | 35.0% |
| !! | GAAAACTGGTTAGTTTCTGA+AGG | + | chr8.3:61196227-61196246 | MS.gene57486:CDS | 35.0% |
| !!! | AGCATTTTGGCTTATCTTGA+TGG | + | chr8.3:61196635-61196654 | MS.gene57486:CDS | 35.0% |
| !!! | TCAAGCAACTTTTTAGTTCC+TGG | - | chr8.3:61197157-61197176 | None:intergenic | 35.0% |
| AAGAGAAACCAGAGTTTCCT+CGG | - | chr8.3:61196717-61196736 | None:intergenic | 40.0% | |
| AGAGAGGTAAAAGGGTTTCA+AGG | + | chr8.3:61195639-61195658 | MS.gene57486:CDS | 40.0% | |
| AGGTAAAAGGGTTTCAAGGT+AGG | + | chr8.3:61195643-61195662 | MS.gene57486:CDS | 40.0% | |
| AGGTCTGTTATATCAGAAGG+CGG | + | chr8.3:61196605-61196624 | MS.gene57486:CDS | 40.0% | |
| ATTGAGGAATCTCGTTGCAT+CGG | + | chr8.3:61196688-61196707 | MS.gene57486:CDS | 40.0% | |
| CAAGCAATGAGAATCTGAGA+AGG | + | chr8.3:61196585-61196604 | MS.gene57486:CDS | 40.0% | |
| CAGCAACATCAACAAGGATA+AGG | + | chr8.3:61196153-61196172 | MS.gene57486:CDS | 40.0% | |
| CTTATTGAGATGGAGATTCC+AGG | + | chr8.3:61197136-61197155 | MS.gene57486:CDS | 40.0% | |
| GAAGAATATTTCGGCTGTTC+CGG | + | chr8.3:61196445-61196464 | MS.gene57486:CDS | 40.0% | |
| GGAATCGAAACACAAAGCTT+TGG | + | chr8.3:61196040-61196059 | MS.gene57486:CDS | 40.0% | |
| TAAAAGGGTTTCAAGGTAGG+TGG | + | chr8.3:61195646-61195665 | MS.gene57486:CDS | 40.0% | |
| TGCTCTTACAAAAGCAAGAG+AGG | + | chr8.3:61195623-61195642 | MS.gene57486:CDS | 40.0% | |
| TGCTTACAGCAACATCAACA+AGG | + | chr8.3:61196147-61196166 | MS.gene57486:CDS | 40.0% | |
| ! | GTGTTTTGCCACCACTTATA+AGG | + | chr8.3:61196249-61196268 | MS.gene57486:CDS | 40.0% |
| ! | TCAACAAGCCTTATAAGTGG+TGG | - | chr8.3:61196260-61196279 | None:intergenic | 40.0% |
| !! | AAATACGCAGTTTGTTGCCT+TGG | + | chr8.3:61197043-61197062 | MS.gene57486:CDS | 40.0% |
| !!! | ATGTTTTGGCTGCTTTTTGC+AGG | + | chr8.3:61196099-61196118 | MS.gene57486:CDS | 40.0% |
| AATCTGCTGTTGGTGCATTG+AGG | + | chr8.3:61196672-61196691 | MS.gene57486:CDS | 45.0% | |
| ACAGCAGATTCTTGAGGAAG+TGG | - | chr8.3:61196662-61196681 | None:intergenic | 45.0% | |
| ACCTGACAAAGCTCGACTAA+CGG | - | chr8.3:61196386-61196405 | None:intergenic | 45.0% | |
| ACGCCTTCAAATCGGTCATT+TGG | + | chr8.3:61196019-61196038 | MS.gene57486:CDS | 45.0% | |
| CTTCCTCAAGAATCTGCTGT+TGG | + | chr8.3:61196662-61196681 | MS.gene57486:CDS | 45.0% | |
| TCTGTCGCAAAATCGAAGAG+AGG | + | chr8.3:61196949-61196968 | MS.gene57486:CDS | 45.0% | |
| TTACTCGCACGCCTTCAAAT+CGG | + | chr8.3:61196011-61196030 | MS.gene57486:CDS | 45.0% | |
| ! | CAGGAGCAATGTTGTTGCTT+TGG | + | chr8.3:61196118-61196137 | MS.gene57486:CDS | 45.0% |
| ! | GAGGAAACTCTGGTTTCTCT+TGG | + | chr8.3:61196716-61196735 | MS.gene57486:CDS | 45.0% |
| ! | TTAGTCGAGCTTTGTCAGGT+TGG | + | chr8.3:61196386-61196405 | MS.gene57486:CDS | 45.0% |
| ! | TTCGCAGTGTTTTGTGGACT+AGG | - | chr8.3:61197022-61197041 | None:intergenic | 45.0% |
| !!! | AGACAGGTTTTGGCTGAAGA+AGG | + | chr8.3:61196473-61196492 | MS.gene57486:CDS | 45.0% |
| ACTCGTGCAATCGTTGGACA+TGG | + | chr8.3:61196350-61196369 | MS.gene57486:CDS | 50.0% | |
| GACACAACTCGTGCAATCGT+TGG | + | chr8.3:61196344-61196363 | MS.gene57486:CDS | 50.0% | |
| GCACCAACAGCAGATTCTTG+AGG | - | chr8.3:61196668-61196687 | None:intergenic | 50.0% | |
| GCCAAAACCTGTCTCACTTC+CGG | - | chr8.3:61196467-61196486 | None:intergenic | 50.0% | |
| GTGCAATCGTTGGACATGGA+GGG | + | chr8.3:61196354-61196373 | MS.gene57486:CDS | 50.0% | |
| TGCAATCGTTGGACATGGAG+GGG | + | chr8.3:61196355-61196374 | MS.gene57486:CDS | 50.0% | |
| TGCGAAACAATGCGCTGCAT+CGG | + | chr8.3:61196784-61196803 | MS.gene57486:CDS | 50.0% | |
| TGCTCTCTTGTTGAATCCGG+TGG | + | chr8.3:61196197-61196216 | MS.gene57486:CDS | 50.0% | |
| ! | ATCGGTTTCCGAGGAAACTC+TGG | + | chr8.3:61196706-61196725 | MS.gene57486:CDS | 50.0% |
| ! | TCCGGAAGTGAGACAGGTTT+TGG | + | chr8.3:61196463-61196482 | MS.gene57486:CDS | 50.0% |
| ! | TCCGTTAGTCGAGCTTTGTC+AGG | + | chr8.3:61196382-61196401 | MS.gene57486:CDS | 50.0% |
| !! | GGCTGAAGAAGGCGTTGTTA+AGG | + | chr8.3:61196484-61196503 | MS.gene57486:CDS | 50.0% |
| CGTGCAATCGTTGGACATGG+AGG | + | chr8.3:61196353-61196372 | MS.gene57486:CDS | 55.0% | |
| ! | CCAGTTTTCGCAGCTACCAC+CGG | - | chr8.3:61196216-61196235 | None:intergenic | 55.0% |
| !! | AGGCGGCGTTCGAAGCATTT+TGG | + | chr8.3:61196622-61196641 | MS.gene57486:CDS | 55.0% |
| !! | GCGCTGCATCGGTTATATGC+AGG | + | chr8.3:61196795-61196814 | MS.gene57486:CDS | 55.0% |
| !! | TCTCGTTGCATCGGTTTCCG+AGG | + | chr8.3:61196697-61196716 | MS.gene57486:CDS | 55.0% |
| CCGGTGGTAGCTGCGAAAAC+TGG | + | chr8.3:61196213-61196232 | MS.gene57486:CDS | 60.0% | |
| GGCTGTTCCGGAAGTGAGAC+AGG | + | chr8.3:61196457-61196476 | MS.gene57486:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 61195537 | 61197222 | 61195537 | ID=MS.gene57486 |
| chr8.3 | mRNA | 61195537 | 61197222 | 61195537 | ID=MS.gene57486.t1;Parent=MS.gene57486 |
| chr8.3 | exon | 61195537 | 61197222 | 61195537 | ID=MS.gene57486.t1.exon1;Parent=MS.gene57486.t1 |
| chr8.3 | CDS | 61195537 | 61197222 | 61195537 | ID=cds.MS.gene57486.t1;Parent=MS.gene57486.t1 |
| Gene Sequence |
| Protein sequence |
