Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36899.t1 | XP_013444940.1 | 97.1 | 561 | 16 | 0 | 1 | 561 | 1 | 561 | 4.40E-259 | 903.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36899.t1 | A0A072TP55 | 97.1 | 561 | 16 | 0 | 1 | 561 | 24 | 584 | 3.2e-259 | 903.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene36899 | 0.802707 | 4.94E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene36899 | 0.8052 | 1.50E-49 | -1.69E-46 |
| MS.gene049178 | MS.gene36899 | 0.826823 | 2.20E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene36899 | 0.8131 | 3.04E-51 | -1.69E-46 |
| MS.gene049501 | MS.gene36899 | 0.831087 | 2.04E-55 | -1.69E-46 |
| MS.gene049647 | MS.gene36899 | 0.809007 | 2.34E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene36899 | 0.803734 | 3.03E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene36899 | 0.801337 | 9.44E-49 | -1.69E-46 |
| MS.gene050137 | MS.gene36899 | 0.823937 | 1.06E-53 | -1.69E-46 |
| MS.gene050156 | MS.gene36899 | 0.816716 | 4.79E-52 | -1.69E-46 |
| MS.gene050226 | MS.gene36899 | 0.812322 | 4.49E-51 | -1.69E-46 |
| MS.gene050227 | MS.gene36899 | 0.828704 | 7.75E-55 | -1.69E-46 |
| MS.gene050228 | MS.gene36899 | 0.82547 | 4.61E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene36899 | 0.806628 | 7.50E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene36899 | 0.822577 | 2.20E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene36899 | 0.831143 | 1.97E-55 | -1.69E-46 |
| MS.gene050719 | MS.gene36899 | 0.855257 | 7.12E-62 | -1.69E-46 |
| MS.gene050721 | MS.gene36899 | 0.851783 | 7.09E-61 | -1.69E-46 |
| MS.gene050724 | MS.gene36899 | 0.847035 | 1.49E-59 | -1.69E-46 |
| MS.gene050780 | MS.gene36899 | 0.838309 | 3.10E-57 | -1.69E-46 |
| MS.gene050787 | MS.gene36899 | 0.811102 | 8.28E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene36899 | 0.82167 | 3.57E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene36899 | 0.80483 | 1.79E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene36899 | 0.812983 | 3.22E-51 | -1.69E-46 |
| MS.gene050959 | MS.gene36899 | 0.821111 | 4.80E-53 | -1.69E-46 |
| MS.gene051210 | MS.gene36899 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene36899 | 0.861195 | 1.22E-63 | -1.69E-46 |
| MS.gene051265 | MS.gene36899 | 0.843152 | 1.67E-58 | -1.69E-46 |
| MS.gene051490 | MS.gene36899 | 0.800203 | 1.61E-48 | -1.69E-46 |
| MS.gene051700 | MS.gene36899 | 0.822987 | 1.77E-53 | -1.69E-46 |
| MS.gene051705 | MS.gene36899 | 0.81065 | 1.04E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene36899 | 0.830379 | 3.04E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene36899 | 0.808199 | 3.48E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene36899 | 0.82158 | 3.74E-53 | -1.69E-46 |
| MS.gene051790 | MS.gene36899 | 0.811475 | 6.87E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene36899 | 0.801294 | 9.64E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene36899 | 0.827286 | 1.70E-54 | -1.69E-46 |
| MS.gene052323 | MS.gene36899 | 0.804598 | 2.00E-49 | -1.69E-46 |
| MS.gene052561 | MS.gene36899 | 0.809114 | 2.22E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene36899 | 0.802893 | 4.52E-49 | -1.69E-46 |
| MS.gene052613 | MS.gene36899 | 0.803482 | 3.41E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene36899 | 0.822657 | 2.11E-53 | -1.69E-46 |
| MS.gene052673 | MS.gene36899 | -0.805276 | 1.44E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene36899 | 0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene052896 | MS.gene36899 | 0.827614 | 1.42E-54 | -1.69E-46 |
| MS.gene053054 | MS.gene36899 | -0.842754 | 2.13E-58 | -1.69E-46 |
| MS.gene053221 | MS.gene36899 | 0.846457 | 2.15E-59 | -1.69E-46 |
| MS.gene053226 | MS.gene36899 | 0.837915 | 3.92E-57 | -1.69E-46 |
| MS.gene053811 | MS.gene36899 | 0.801834 | 7.47E-49 | -1.69E-46 |
| MS.gene054139 | MS.gene36899 | 0.808108 | 3.64E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene36899 | 0.806906 | 6.55E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene36899 | -0.824185 | 9.26E-54 | -1.69E-46 |
| MS.gene054467 | MS.gene36899 | 0.841776 | 3.87E-58 | -1.69E-46 |
| MS.gene054468 | MS.gene36899 | 0.845947 | 2.95E-59 | -1.69E-46 |
| MS.gene054576 | MS.gene36899 | 0.811188 | 7.93E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene36899 | 0.857679 | 1.39E-62 | -1.69E-46 |
| MS.gene054775 | MS.gene36899 | 0.826823 | 2.20E-54 | -1.69E-46 |
| MS.gene054942 | MS.gene36899 | 0.802574 | 5.26E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene36899 | 0.822245 | 2.63E-53 | -1.69E-46 |
| MS.gene055357 | MS.gene36899 | 0.812789 | 3.55E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene36899 | 0.816753 | 4.70E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene36899 | 0.804205 | 2.42E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene36899 | 0.818881 | 1.55E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene36899 | 0.804357 | 2.25E-49 | -1.69E-46 |
| MS.gene055608 | MS.gene36899 | 0.822356 | 2.48E-53 | -1.69E-46 |
| MS.gene055778 | MS.gene36899 | 0.824997 | 5.96E-54 | -1.69E-46 |
| MS.gene055818 | MS.gene36899 | 0.824523 | 7.71E-54 | -1.69E-46 |
| MS.gene055926 | MS.gene36899 | 0.801788 | 7.63E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene36899 | 0.834136 | 3.57E-56 | -1.69E-46 |
| MS.gene056050 | MS.gene36899 | 0.802728 | 4.89E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene36899 | 0.816124 | 6.50E-52 | -1.69E-46 |
| MS.gene056373 | MS.gene36899 | 0.802945 | 4.41E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene36899 | 0.812443 | 4.23E-51 | -1.69E-46 |
| MS.gene056552 | MS.gene36899 | 0.811311 | 7.46E-51 | -1.69E-46 |
| MS.gene056553 | MS.gene36899 | 0.800432 | 1.45E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene36899 | 0.835935 | 1.26E-56 | -1.69E-46 |
| MS.gene057414 | MS.gene36899 | 0.817984 | 2.48E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene36899 | 0.810382 | 1.19E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene36899 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene058168 | MS.gene36899 | 0.835703 | 1.44E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene36899 | 0.834109 | 3.62E-56 | -1.69E-46 |
| MS.gene058636 | MS.gene36899 | 0.840977 | 6.28E-58 | -1.69E-46 |
| MS.gene058792 | MS.gene36899 | 0.807236 | 5.58E-50 | -1.69E-46 |
| MS.gene058829 | MS.gene36899 | 0.812751 | 3.62E-51 | -1.69E-46 |
| MS.gene059084 | MS.gene36899 | 0.820934 | 5.28E-53 | -1.69E-46 |
| MS.gene059331 | MS.gene36899 | 0.80156 | 8.50E-49 | -1.69E-46 |
| MS.gene059335 | MS.gene36899 | 0.807713 | 4.42E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene36899 | 0.80839 | 3.17E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene36899 | 0.810662 | 1.03E-50 | -1.69E-46 |
| MS.gene059725 | MS.gene36899 | 0.851889 | 6.61E-61 | -1.69E-46 |
| MS.gene060137 | MS.gene36899 | -0.824201 | 9.18E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene36899 | 0.804402 | 2.20E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene36899 | 0.806665 | 7.37E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene36899 | 0.831403 | 1.70E-55 | -1.69E-46 |
| MS.gene060803 | MS.gene36899 | 0.817937 | 2.54E-52 | -1.69E-46 |
| MS.gene060998 | MS.gene36899 | 0.803788 | 2.95E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene36899 | -0.825558 | 4.39E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene36899 | MS.gene052564 | PPI |
| MS.gene41659 | MS.gene36899 | PPI |
| MS.gene013180 | MS.gene36899 | PPI |
| MS.gene055531 | MS.gene36899 | PPI |
| MS.gene36899 | MS.gene28664 | PPI |
| MS.gene36899 | MS.gene32213 | PPI |
| MS.gene06253 | MS.gene36899 | PPI |
| MS.gene052565 | MS.gene36899 | PPI |
| MS.gene33842 | MS.gene36899 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36899.t1 | MTR_8g032570 | 96.791 | 561 | 18 | 0 | 1 | 561 | 24 | 584 | 0.0 | 1092 |
| MS.gene36899.t1 | MTR_8g032570 | 96.791 | 561 | 18 | 0 | 1 | 561 | 1 | 561 | 0.0 | 1092 |
| MS.gene36899.t1 | MTR_7g076220 | 77.660 | 564 | 119 | 3 | 1 | 561 | 1 | 560 | 0.0 | 878 |
| MS.gene36899.t1 | MTR_3g098730 | 35.382 | 537 | 321 | 13 | 37 | 560 | 30 | 553 | 3.07e-80 | 262 |
| MS.gene36899.t1 | MTR_4g091320 | 29.947 | 561 | 352 | 14 | 25 | 559 | 27 | 572 | 4.58e-63 | 217 |
| MS.gene36899.t1 | MTR_5g092900 | 52.459 | 122 | 19 | 4 | 372 | 491 | 32 | 116 | 4.21e-24 | 97.8 |
| MS.gene36899.t1 | MTR_1g018180 | 29.060 | 234 | 158 | 5 | 238 | 466 | 101 | 331 | 8.82e-20 | 91.3 |
| MS.gene36899.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 484 | 721 | 2.09e-17 | 86.3 |
| MS.gene36899.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 480 | 717 | 2.12e-17 | 86.3 |
| MS.gene36899.t1 | MTR_1g069845 | 28.862 | 246 | 162 | 7 | 238 | 478 | 363 | 600 | 2.15e-17 | 85.9 |
| MS.gene36899.t1 | MTR_4g007850 | 32.237 | 152 | 100 | 3 | 409 | 557 | 364 | 515 | 7.36e-16 | 80.9 |
| MS.gene36899.t1 | MTR_7g097020 | 28.512 | 242 | 154 | 9 | 238 | 469 | 522 | 754 | 1.24e-15 | 80.9 |
| MS.gene36899.t1 | MTR_7g097020 | 31.034 | 203 | 134 | 4 | 337 | 536 | 496 | 695 | 5.12e-11 | 65.9 |
| MS.gene36899.t1 | MTR_3g103050 | 26.577 | 222 | 147 | 7 | 238 | 448 | 91 | 307 | 4.02e-15 | 77.4 |
| MS.gene36899.t1 | MTR_7g078330 | 27.240 | 279 | 194 | 7 | 201 | 474 | 370 | 644 | 2.56e-14 | 76.3 |
| MS.gene36899.t1 | MTR_7g078330 | 27.018 | 285 | 195 | 8 | 146 | 425 | 361 | 637 | 1.64e-12 | 70.5 |
| MS.gene36899.t1 | MTR_3g466220 | 29.949 | 197 | 123 | 5 | 180 | 365 | 566 | 758 | 4.08e-11 | 66.2 |
| MS.gene36899.t1 | MTR_3g466220 | 29.949 | 197 | 123 | 5 | 180 | 365 | 545 | 737 | 4.79e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36899.t1 | AT2G45720 | 67.023 | 561 | 177 | 4 | 1 | 561 | 1 | 553 | 0.0 | 754 |
| MS.gene36899.t1 | AT2G45720 | 67.023 | 561 | 177 | 4 | 1 | 561 | 1 | 553 | 0.0 | 754 |
| MS.gene36899.t1 | AT2G45720 | 67.023 | 561 | 177 | 4 | 1 | 561 | 1 | 553 | 0.0 | 754 |
| MS.gene36899.t1 | AT2G45720 | 67.023 | 561 | 177 | 4 | 1 | 561 | 1 | 553 | 0.0 | 754 |
| MS.gene36899.t1 | AT2G45720 | 67.023 | 561 | 177 | 4 | 1 | 561 | 1 | 553 | 0.0 | 754 |
| MS.gene36899.t1 | AT1G01830 | 63.036 | 560 | 201 | 4 | 3 | 560 | 19 | 574 | 0.0 | 674 |
| MS.gene36899.t1 | AT1G01830 | 63.036 | 560 | 201 | 4 | 3 | 560 | 19 | 574 | 0.0 | 674 |
| MS.gene36899.t1 | AT1G01830 | 63.036 | 560 | 201 | 4 | 3 | 560 | 19 | 574 | 0.0 | 674 |
| MS.gene36899.t1 | AT5G50900 | 37.736 | 530 | 316 | 8 | 40 | 560 | 30 | 554 | 3.51e-102 | 319 |
| MS.gene36899.t1 | AT1G61350 | 27.605 | 547 | 361 | 12 | 38 | 559 | 32 | 568 | 6.21e-60 | 209 |
| MS.gene36899.t1 | AT2G05810 | 32.222 | 540 | 340 | 12 | 37 | 558 | 41 | 572 | 1.61e-49 | 180 |
| MS.gene36899.t1 | AT2G05810 | 32.222 | 540 | 340 | 12 | 37 | 558 | 41 | 572 | 1.61e-49 | 180 |
| MS.gene36899.t1 | AT5G58680 | 27.686 | 242 | 167 | 5 | 234 | 470 | 99 | 337 | 3.85e-21 | 95.5 |
| MS.gene36899.t1 | AT3G01400 | 26.087 | 230 | 162 | 5 | 238 | 462 | 105 | 331 | 3.36e-16 | 80.9 |
| MS.gene36899.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 540 | 818 | 7.09e-15 | 78.6 |
| MS.gene36899.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 537 | 815 | 7.65e-15 | 78.2 |
| MS.gene36899.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 537 | 815 | 7.65e-15 | 78.2 |
| MS.gene36899.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 348 | 626 | 3.00e-14 | 76.3 |
| MS.gene36899.t1 | AT2G23140 | 25.000 | 324 | 197 | 7 | 148 | 470 | 348 | 626 | 3.00e-14 | 76.3 |
| MS.gene36899.t1 | AT2G28830 | 25.697 | 323 | 184 | 7 | 151 | 464 | 280 | 555 | 1.60e-12 | 70.5 |
| MS.gene36899.t1 | AT5G67340 | 27.875 | 287 | 192 | 10 | 146 | 425 | 415 | 693 | 4.19e-12 | 69.3 |
| MS.gene36899.t1 | AT5G67340 | 27.875 | 287 | 192 | 10 | 146 | 425 | 415 | 693 | 4.19e-12 | 69.3 |
| MS.gene36899.t1 | AT3G20170 | 26.115 | 314 | 222 | 7 | 185 | 493 | 94 | 402 | 9.29e-12 | 67.8 |
Find 87 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATGATCATTTCAAAAT+TGG | 0.161848 | 8.4:+63228567 | MS.gene36899:CDS |
| AGTGATCTTGATGCATTAAT+TGG | 0.208436 | 8.4:+63228754 | MS.gene36899:CDS |
| TAAGCTTGATTTGAATTTAA+AGG | 0.223787 | 8.4:+63228777 | MS.gene36899:CDS |
| GATAAATCTGATAGCTTTGA+TGG | 0.243067 | 8.4:-63228598 | None:intergenic |
| AGGCGGCGTTCGAAGCATTT+TGG | 0.264072 | 8.4:+63229521 | MS.gene36899:CDS |
| TGATAAAAGTGTGCCGAATT+TGG | 0.271000 | 8.4:+63229881 | MS.gene36899:CDS |
| CTTAACAATGGAATGTTGTT+AGG | 0.279304 | 8.4:+63229420 | MS.gene36899:CDS |
| ACAAAAGCAAGAGAGGTAAA+AGG | 0.285811 | 8.4:+63228529 | MS.gene36899:CDS |
| ATGATTTCATATGGAGCAAT+AGG | 0.295280 | 8.4:+63229999 | MS.gene36899:CDS |
| TTGTACTTTGAAGAATATTT+CGG | 0.300330 | 8.4:+63229335 | MS.gene36899:CDS |
| TCCGGAAGTGAGACAGGTTT+TGG | 0.307249 | 8.4:+63229362 | MS.gene36899:CDS |
| CTTATAAGGCTTGTTGAATC+AGG | 0.333515 | 8.4:+63229162 | MS.gene36899:CDS |
| TTCAAAGAATGCACTTTGTA+AGG | 0.336893 | 8.4:+63228636 | MS.gene36899:CDS |
| GAAGAATATTTCGGCTGTTC+CGG | 0.352070 | 8.4:+63229344 | MS.gene36899:CDS |
| AAATACGCAGTTTGTTGCCT+TGG | 0.353804 | 8.4:+63229942 | MS.gene36899:CDS |
| CAGGAGCAATGTTGTTGCTT+TGG | 0.355138 | 8.4:+63229017 | MS.gene36899:CDS |
| TGAAACACATTGCAATATAA+AGG | 0.363878 | 8.4:+63228885 | MS.gene36899:CDS |
| GAGGAAACTCTGGTTTCTCT+TGG | 0.367522 | 8.4:+63229615 | MS.gene36899:CDS |
| TCCGTTAGTCGAGCTTTGTC+AGG | 0.385091 | 8.4:+63229281 | MS.gene36899:CDS |
| ATTCCAAATGACCGATTTGA+AGG | 0.386702 | 8.4:-63228921 | None:intergenic |
| AGAAGATGAGAAGAATGTTT+TGG | 0.395990 | 8.4:+63228984 | MS.gene36899:CDS |
| ACTTTGTAAGGAACAATTGC+AGG | 0.398251 | 8.4:+63228648 | MS.gene36899:CDS |
| GCCAAAACCTGTCTCACTTC+CGG | 0.399317 | 8.4:-63229363 | None:intergenic |
| ACGCCTTCAAATCGGTCATT+TGG | 0.403237 | 8.4:+63228918 | MS.gene36899:CDS |
| ATTAGCTGAGTTATGTTTGA+AGG | 0.406726 | 8.4:+63228702 | MS.gene36899:CDS |
| GGAATCGAAACACAAAGCTT+TGG | 0.406964 | 8.4:+63228939 | MS.gene36899:CDS |
| ACAGCAGATTCTTGAGGAAA+TGG | 0.410112 | 8.4:-63229558 | None:intergenic |
| CCGGTGGTAGCTGCGAAAAC+TGG | 0.410752 | 8.4:+63229112 | MS.gene36899:CDS |
| TCAAGCAATTGAACCAAATT+CGG | 0.411317 | 8.4:-63229894 | None:intergenic |
| ATTGAGATGAAGAAAATGTT+AGG | 0.426418 | 8.4:+63229729 | MS.gene36899:CDS |
| ATATGCTCTCTTGTTGAATC+CGG | 0.443973 | 8.4:+63229093 | MS.gene36899:CDS |
| CTTATTGAGATGGAGATTCC+AGG | 0.448410 | 8.4:+63230035 | MS.gene36899:CDS |
| CAAAAGCAAGAGAGGTAAAA+GGG | 0.451511 | 8.4:+63228530 | MS.gene36899:CDS |
| CAGCAACATCAACAAGGATA+AGG | 0.452305 | 8.4:+63229052 | MS.gene36899:CDS |
| AAGAAAATGTTAGGTGAAGT+TGG | 0.452479 | 8.4:+63229738 | MS.gene36899:CDS |
| AGGCTATTGTTTCTCTTCAA+AGG | 0.458318 | 8.4:+63229205 | MS.gene36899:CDS |
| AGGTAAAAGGGTTTCAAGGT+AGG | 0.459525 | 8.4:+63228542 | MS.gene36899:CDS |
| AGCAACATCAACAAGGATAA+GGG | 0.463755 | 8.4:+63229053 | MS.gene36899:CDS |
| GGCTGTTCCGGAAGTGAGAC+AGG | 0.464868 | 8.4:+63229356 | MS.gene36899:CDS |
| GGCTGAAGAAGGCGTTGTTA+AGG | 0.466264 | 8.4:+63229383 | MS.gene36899:CDS |
| TGCTTGAAAGATTGGAAAGA+GGG | 0.471530 | 8.4:+63230069 | MS.gene36899:CDS |
| TTACTCGCACGCCTTCAAAT+CGG | 0.478346 | 8.4:+63228910 | MS.gene36899:CDS |
| AAGAGGTTGATGATTTCATA+TGG | 0.478705 | 8.4:+63229990 | MS.gene36899:CDS |
| AGAGAGGTAAAAGGGTTTCA+AGG | 0.482668 | 8.4:+63228538 | MS.gene36899:CDS |
| CTATTGATCAACATGCTTGA+GGG | 0.487366 | 8.4:+63229771 | MS.gene36899:CDS |
| TGATTTCATATGGAGCAATA+GGG | 0.489291 | 8.4:+63230000 | MS.gene36899:CDS |
| TCAAGCATGTTGATCAATAG+AGG | 0.491275 | 8.4:-63229768 | None:intergenic |
| TTCAACAGAAGAATGGTTGT+TGG | 0.494650 | 8.4:+63228477 | MS.gene36899:CDS |
| TAGTGCTATTGCTAAAGAAA+AGG | 0.499049 | 8.4:+63229185 | MS.gene36899:CDS |
| ATCGGTTTCCGAGGAAACTC+TGG | 0.509299 | 8.4:+63229605 | MS.gene36899:CDS |
| ATACATGTTCAACAGAAGAA+TGG | 0.512048 | 8.4:+63228470 | MS.gene36899:CDS |
| TCTATTGATCAACATGCTTG+AGG | 0.513990 | 8.4:+63229770 | MS.gene36899:CDS |
| TTTCCTCAAGAATCTGCTGT+TGG | 0.517287 | 8.4:+63229561 | MS.gene36899:CDS |
| TTGCTTGAAAGATTGGAAAG+AGG | 0.535251 | 8.4:+63230068 | MS.gene36899:CDS |
| GACACAACTCGTGCAATCGT+TGG | 0.538831 | 8.4:+63229243 | MS.gene36899:CDS |
| TGCGAAACAATGCGCTGCAT+CGG | 0.539824 | 8.4:+63229683 | MS.gene36899:CDS |
| ACCTGACAAAGCTCGACTAA+CGG | 0.545060 | 8.4:-63229282 | None:intergenic |
| TCTTCAAAGGTTGTCTATGA+CGG | 0.545473 | 8.4:+63229218 | MS.gene36899:CDS |
| TCTCGTTGCATCGGTTTCCG+AGG | 0.548267 | 8.4:+63229596 | MS.gene36899:CDS |
| GCGCTGCATCGGTTATATGC+AGG | 0.549822 | 8.4:+63229694 | MS.gene36899:CDS |
| TCTGAAGAAGCTTATTGAGA+TGG | 0.553377 | 8.4:+63230025 | MS.gene36899:CDS |
| ATTGAGGAATCTCGTTGCAT+CGG | 0.553997 | 8.4:+63229587 | MS.gene36899:CDS |
| ATGATCAATCTTCTTAACAA+TGG | 0.556218 | 8.4:+63229408 | MS.gene36899:CDS |
| AAGAGAAACCAGAGTTTCCT+CGG | 0.556662 | 8.4:-63229613 | None:intergenic |
| TTAGTCGAGCTTTGTCAGGT+TGG | 0.558482 | 8.4:+63229285 | MS.gene36899:CDS |
| TGCTCTTACAAAAGCAAGAG+AGG | 0.561490 | 8.4:+63228522 | MS.gene36899:CDS |
| TGCAATCGTTGGACATGGAG+GGG | 0.562940 | 8.4:+63229254 | MS.gene36899:CDS |
| GAAAACTGGTTAGTTTCTGA+AGG | 0.563650 | 8.4:+63229126 | MS.gene36899:CDS |
| GTGCAATCGTTGGACATGGA+GGG | 0.565053 | 8.4:+63229253 | MS.gene36899:CDS |
| TGCTCTCTTGTTGAATCCGG+TGG | 0.570371 | 8.4:+63229096 | MS.gene36899:CDS |
| AGAAGGTCTGTTATATCAGA+AGG | 0.575080 | 8.4:+63229501 | MS.gene36899:CDS |
| CTTCTTGTAAGAAATGCAAG+AGG | 0.577874 | 8.4:+63229973 | MS.gene36899:CDS |
| TAAAAGGGTTTCAAGGTAGG+TGG | 0.577886 | 8.4:+63228545 | MS.gene36899:CDS |
| AAATCGAAGAGAGGTTAAGA+AGG | 0.584354 | 8.4:+63229857 | MS.gene36899:CDS |
| ACTCGTGCAATCGTTGGACA+TGG | 0.608350 | 8.4:+63229249 | MS.gene36899:CDS |
| GATTCAACAAGCCTTATAAG+TGG | 0.616500 | 8.4:-63229159 | None:intergenic |
| TACAAGAAGAAAGTGATCCA+AGG | 0.619451 | 8.4:-63229959 | None:intergenic |
| TACTATACTATCTTCCACCA+TGG | 0.626237 | 8.4:-63228434 | None:intergenic |
| TCAACAAGCCTTATAAGTGG+TGG | 0.632485 | 8.4:-63229156 | None:intergenic |
| CAAGCAATGAGAATCTGAGA+AGG | 0.635445 | 8.4:+63229484 | MS.gene36899:CDS |
| CGTGCAATCGTTGGACATGG+AGG | 0.635524 | 8.4:+63229252 | MS.gene36899:CDS |
| TTCAAAACATGAACTAAACA+AGG | 0.642239 | 8.4:-63229645 | None:intergenic |
| GCACCAACAGCAGATTCTTG+AGG | 0.646827 | 8.4:-63229564 | None:intergenic |
| AATCTGCTGTTGGTGCATTG+AGG | 0.654433 | 8.4:+63229571 | MS.gene36899:CDS |
| TGCTTACAGCAACATCAACA+AGG | 0.669898 | 8.4:+63229046 | MS.gene36899:CDS |
| TCTGTCGCAAAATCGAAGAG+AGG | 0.676427 | 8.4:+63229848 | MS.gene36899:CDS |
| AGGTCTGTTATATCAGAAGG+CGG | 0.697553 | 8.4:+63229504 | MS.gene36899:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAAATGATCATTTCAAAAT+TGG | + | chr8.4:63228567-63228586 | MS.gene36899:CDS | 20.0% |
| !!! | TAAGCTTGATTTGAATTTAA+AGG | + | chr8.4:63228777-63228796 | MS.gene36899:CDS | 20.0% |
| !!! | TTGTACTTTGAAGAATATTT+CGG | + | chr8.4:63229335-63229354 | MS.gene36899:CDS | 20.0% |
| ! | ATGATCAATCTTCTTAACAA+TGG | + | chr8.4:63229408-63229427 | MS.gene36899:CDS | 25.0% |
| ! | ATTGAGATGAAGAAAATGTT+AGG | + | chr8.4:63229729-63229748 | MS.gene36899:CDS | 25.0% |
| ! | TAAAAAGTTGCTTGAAAGAT+TGG | + | chr8.4:63230061-63230080 | MS.gene36899:CDS | 25.0% |
| ! | TGAAACACATTGCAATATAA+AGG | + | chr8.4:63228885-63228904 | MS.gene36899:CDS | 25.0% |
| ! | TGTTTGAAGGAAAAATATGA+AGG | + | chr8.4:63228715-63228734 | MS.gene36899:CDS | 25.0% |
| ! | TTCAAAACATGAACTAAACA+AGG | - | chr8.4:63229648-63229667 | None:intergenic | 25.0% |
| !!! | TTAGTTCATGTTTTGAAATC+AGG | + | chr8.4:63229651-63229670 | MS.gene36899:CDS | 25.0% |
| AAGAAAATGTTAGGTGAAGT+TGG | + | chr8.4:63229738-63229757 | MS.gene36899:CDS | 30.0% | |
| AGTGATCTTGATGCATTAAT+TGG | + | chr8.4:63228754-63228773 | MS.gene36899:CDS | 30.0% | |
| ATACATGTTCAACAGAAGAA+TGG | + | chr8.4:63228470-63228489 | MS.gene36899:CDS | 30.0% | |
| ATGATTTCATATGGAGCAAT+AGG | + | chr8.4:63229999-63230018 | MS.gene36899:CDS | 30.0% | |
| ATTAGCTGAGTTATGTTTGA+AGG | + | chr8.4:63228702-63228721 | MS.gene36899:CDS | 30.0% | |
| CTTAACAATGGAATGTTGTT+AGG | + | chr8.4:63229420-63229439 | MS.gene36899:CDS | 30.0% | |
| TCAAGCAATTGAACCAAATT+CGG | - | chr8.4:63229897-63229916 | None:intergenic | 30.0% | |
| TGATTTCATATGGAGCAATA+GGG | + | chr8.4:63230000-63230019 | MS.gene36899:CDS | 30.0% | |
| TTAACAAAAACTGGTGTACT+TGG | + | chr8.4:63228811-63228830 | MS.gene36899:CDS | 30.0% | |
| TTCAAAGAATGCACTTTGTA+AGG | + | chr8.4:63228636-63228655 | MS.gene36899:CDS | 30.0% | |
| ! | AGAAGATGAGAAGAATGTTT+TGG | + | chr8.4:63228984-63229003 | MS.gene36899:CDS | 30.0% |
| ! | AGTGCATTCTTTGAAAAACA+AGG | - | chr8.4:63228631-63228650 | None:intergenic | 30.0% |
| ! | GTGCATTCTTTGAAAAACAA+GGG | - | chr8.4:63228630-63228649 | None:intergenic | 30.0% |
| ! | TAGTGCTATTGCTAAAGAAA+AGG | + | chr8.4:63229185-63229204 | MS.gene36899:CDS | 30.0% |
| !! | AAGAGGTTGATGATTTCATA+TGG | + | chr8.4:63229990-63230009 | MS.gene36899:CDS | 30.0% |
| !! | GATAAATCTGATAGCTTTGA+TGG | - | chr8.4:63228601-63228620 | None:intergenic | 30.0% |
| !! | TGTAGCTTGTTAACAAAAAC+TGG | + | chr8.4:63228802-63228821 | MS.gene36899:CDS | 30.0% |
| !!! | GTTTTGAAATCAGGATCATT+AGG | + | chr8.4:63229660-63229679 | MS.gene36899:CDS | 30.0% |
| !!! | TATTTTTTCGCAGTGTTTTG+TGG | - | chr8.4:63229927-63229946 | None:intergenic | 30.0% |
| AAATCGAAGAGAGGTTAAGA+AGG | + | chr8.4:63229857-63229876 | MS.gene36899:CDS | 35.0% | |
| ACAAAAGCAAGAGAGGTAAA+AGG | + | chr8.4:63228529-63228548 | MS.gene36899:CDS | 35.0% | |
| ACTTTGTAAGGAACAATTGC+AGG | + | chr8.4:63228648-63228667 | MS.gene36899:CDS | 35.0% | |
| AGAAGGTCTGTTATATCAGA+AGG | + | chr8.4:63229501-63229520 | MS.gene36899:CDS | 35.0% | |
| AGCAACATCAACAAGGATAA+GGG | + | chr8.4:63229053-63229072 | MS.gene36899:CDS | 35.0% | |
| ATATGCTCTCTTGTTGAATC+CGG | + | chr8.4:63229093-63229112 | MS.gene36899:CDS | 35.0% | |
| CAAAAGCAAGAGAGGTAAAA+GGG | + | chr8.4:63228530-63228549 | MS.gene36899:CDS | 35.0% | |
| CTATTGATCAACATGCTTGA+GGG | + | chr8.4:63229771-63229790 | MS.gene36899:CDS | 35.0% | |
| CTTCTTGTAAGAAATGCAAG+AGG | + | chr8.4:63229973-63229992 | MS.gene36899:CDS | 35.0% | |
| TACAAGAAGAAAGTGATCCA+AGG | - | chr8.4:63229962-63229981 | None:intergenic | 35.0% | |
| TCAAGCATGTTGATCAATAG+AGG | - | chr8.4:63229771-63229790 | None:intergenic | 35.0% | |
| TCTATTGATCAACATGCTTG+AGG | + | chr8.4:63229770-63229789 | MS.gene36899:CDS | 35.0% | |
| TCTGAAGAAGCTTATTGAGA+TGG | + | chr8.4:63230025-63230044 | MS.gene36899:CDS | 35.0% | |
| TCTTCAAAGGTTGTCTATGA+CGG | + | chr8.4:63229218-63229237 | MS.gene36899:CDS | 35.0% | |
| TGCTTGAAAGATTGGAAAGA+GGG | + | chr8.4:63230069-63230088 | MS.gene36899:CDS | 35.0% | |
| TTCAACAGAAGAATGGTTGT+TGG | + | chr8.4:63228477-63228496 | MS.gene36899:CDS | 35.0% | |
| TTGCTTGAAAGATTGGAAAG+AGG | + | chr8.4:63230068-63230087 | MS.gene36899:CDS | 35.0% | |
| ! | AGGCTATTGTTTCTCTTCAA+AGG | + | chr8.4:63229205-63229224 | MS.gene36899:CDS | 35.0% |
| ! | CATTCTTTGAAAAACAAGGG+TGG | - | chr8.4:63228627-63228646 | None:intergenic | 35.0% |
| ! | GATTCAACAAGCCTTATAAG+TGG | - | chr8.4:63229162-63229181 | None:intergenic | 35.0% |
| ! | TGATAAAAGTGTGCCGAATT+TGG | + | chr8.4:63229881-63229900 | MS.gene36899:CDS | 35.0% |
| !! | ATTCCAAATGACCGATTTGA+AGG | - | chr8.4:63228924-63228943 | None:intergenic | 35.0% |
| !! | CTTATAAGGCTTGTTGAATC+AGG | + | chr8.4:63229162-63229181 | MS.gene36899:CDS | 35.0% |
| !! | CTTGCTTTTGTAAGAGCAAT+AGG | - | chr8.4:63228520-63228539 | None:intergenic | 35.0% |
| !! | GAAAACTGGTTAGTTTCTGA+AGG | + | chr8.4:63229126-63229145 | MS.gene36899:CDS | 35.0% |
| !!! | AGCATTTTGGCTTATCTTGA+TGG | + | chr8.4:63229534-63229553 | MS.gene36899:CDS | 35.0% |
| !!! | TCAAGCAACTTTTTAGTTCC+TGG | - | chr8.4:63230056-63230075 | None:intergenic | 35.0% |
| AAGAGAAACCAGAGTTTCCT+CGG | - | chr8.4:63229616-63229635 | None:intergenic | 40.0% | |
| ACAGCAGATTCTTGAGGAAA+TGG | - | chr8.4:63229561-63229580 | None:intergenic | 40.0% | |
| AGAGAGGTAAAAGGGTTTCA+AGG | + | chr8.4:63228538-63228557 | MS.gene36899:CDS | 40.0% | |
| AGGTAAAAGGGTTTCAAGGT+AGG | + | chr8.4:63228542-63228561 | MS.gene36899:CDS | 40.0% | |
| AGGTCTGTTATATCAGAAGG+CGG | + | chr8.4:63229504-63229523 | MS.gene36899:CDS | 40.0% | |
| ATTGAGGAATCTCGTTGCAT+CGG | + | chr8.4:63229587-63229606 | MS.gene36899:CDS | 40.0% | |
| CAAGCAATGAGAATCTGAGA+AGG | + | chr8.4:63229484-63229503 | MS.gene36899:CDS | 40.0% | |
| CAGCAACATCAACAAGGATA+AGG | + | chr8.4:63229052-63229071 | MS.gene36899:CDS | 40.0% | |
| CTTATTGAGATGGAGATTCC+AGG | + | chr8.4:63230035-63230054 | MS.gene36899:CDS | 40.0% | |
| GAAGAATATTTCGGCTGTTC+CGG | + | chr8.4:63229344-63229363 | MS.gene36899:CDS | 40.0% | |
| GGAATCGAAACACAAAGCTT+TGG | + | chr8.4:63228939-63228958 | MS.gene36899:CDS | 40.0% | |
| TAAAAGGGTTTCAAGGTAGG+TGG | + | chr8.4:63228545-63228564 | MS.gene36899:CDS | 40.0% | |
| TGCTCTTACAAAAGCAAGAG+AGG | + | chr8.4:63228522-63228541 | MS.gene36899:CDS | 40.0% | |
| TGCTTACAGCAACATCAACA+AGG | + | chr8.4:63229046-63229065 | MS.gene36899:CDS | 40.0% | |
| TTTCCTCAAGAATCTGCTGT+TGG | + | chr8.4:63229561-63229580 | MS.gene36899:CDS | 40.0% | |
| ! | GTGTTTTGCCACCACTTATA+AGG | + | chr8.4:63229148-63229167 | MS.gene36899:CDS | 40.0% |
| ! | TCAACAAGCCTTATAAGTGG+TGG | - | chr8.4:63229159-63229178 | None:intergenic | 40.0% |
| !! | AAATACGCAGTTTGTTGCCT+TGG | + | chr8.4:63229942-63229961 | MS.gene36899:CDS | 40.0% |
| !!! | ATGTTTTGGCTGCTTTTTGC+AGG | + | chr8.4:63228998-63229017 | MS.gene36899:CDS | 40.0% |
| AATCTGCTGTTGGTGCATTG+AGG | + | chr8.4:63229571-63229590 | MS.gene36899:CDS | 45.0% | |
| ACCTGACAAAGCTCGACTAA+CGG | - | chr8.4:63229285-63229304 | None:intergenic | 45.0% | |
| ACGCCTTCAAATCGGTCATT+TGG | + | chr8.4:63228918-63228937 | MS.gene36899:CDS | 45.0% | |
| TCTGTCGCAAAATCGAAGAG+AGG | + | chr8.4:63229848-63229867 | MS.gene36899:CDS | 45.0% | |
| TTACTCGCACGCCTTCAAAT+CGG | + | chr8.4:63228910-63228929 | MS.gene36899:CDS | 45.0% | |
| ! | CAGGAGCAATGTTGTTGCTT+TGG | + | chr8.4:63229017-63229036 | MS.gene36899:CDS | 45.0% |
| ! | GAGGAAACTCTGGTTTCTCT+TGG | + | chr8.4:63229615-63229634 | MS.gene36899:CDS | 45.0% |
| ! | TTAGTCGAGCTTTGTCAGGT+TGG | + | chr8.4:63229285-63229304 | MS.gene36899:CDS | 45.0% |
| ! | TTCGCAGTGTTTTGTGGACT+AGG | - | chr8.4:63229921-63229940 | None:intergenic | 45.0% |
| !!! | AGACAGGTTTTGGCTGAAGA+AGG | + | chr8.4:63229372-63229391 | MS.gene36899:CDS | 45.0% |
| ACTCGTGCAATCGTTGGACA+TGG | + | chr8.4:63229249-63229268 | MS.gene36899:CDS | 50.0% | |
| GACACAACTCGTGCAATCGT+TGG | + | chr8.4:63229243-63229262 | MS.gene36899:CDS | 50.0% | |
| GCACCAACAGCAGATTCTTG+AGG | - | chr8.4:63229567-63229586 | None:intergenic | 50.0% | |
| GCCAAAACCTGTCTCACTTC+CGG | - | chr8.4:63229366-63229385 | None:intergenic | 50.0% | |
| GTGCAATCGTTGGACATGGA+GGG | + | chr8.4:63229253-63229272 | MS.gene36899:CDS | 50.0% | |
| TGCAATCGTTGGACATGGAG+GGG | + | chr8.4:63229254-63229273 | MS.gene36899:CDS | 50.0% | |
| TGCGAAACAATGCGCTGCAT+CGG | + | chr8.4:63229683-63229702 | MS.gene36899:CDS | 50.0% | |
| TGCTCTCTTGTTGAATCCGG+TGG | + | chr8.4:63229096-63229115 | MS.gene36899:CDS | 50.0% | |
| ! | ATCGGTTTCCGAGGAAACTC+TGG | + | chr8.4:63229605-63229624 | MS.gene36899:CDS | 50.0% |
| ! | TCCGGAAGTGAGACAGGTTT+TGG | + | chr8.4:63229362-63229381 | MS.gene36899:CDS | 50.0% |
| ! | TCCGTTAGTCGAGCTTTGTC+AGG | + | chr8.4:63229281-63229300 | MS.gene36899:CDS | 50.0% |
| !! | GGCTGAAGAAGGCGTTGTTA+AGG | + | chr8.4:63229383-63229402 | MS.gene36899:CDS | 50.0% |
| CGTGCAATCGTTGGACATGG+AGG | + | chr8.4:63229252-63229271 | MS.gene36899:CDS | 55.0% | |
| ! | CCAGTTTTCGCAGCTACCAC+CGG | - | chr8.4:63229115-63229134 | None:intergenic | 55.0% |
| !! | AGGCGGCGTTCGAAGCATTT+TGG | + | chr8.4:63229521-63229540 | MS.gene36899:CDS | 55.0% |
| !! | GCGCTGCATCGGTTATATGC+AGG | + | chr8.4:63229694-63229713 | MS.gene36899:CDS | 55.0% |
| !! | TCTCGTTGCATCGGTTTCCG+AGG | + | chr8.4:63229596-63229615 | MS.gene36899:CDS | 55.0% |
| CCGGTGGTAGCTGCGAAAAC+TGG | + | chr8.4:63229112-63229131 | MS.gene36899:CDS | 60.0% | |
| GGCTGTTCCGGAAGTGAGAC+AGG | + | chr8.4:63229356-63229375 | MS.gene36899:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 63228436 | 63230121 | 63228436 | ID=MS.gene36899 |
| chr8.4 | mRNA | 63228436 | 63230121 | 63228436 | ID=MS.gene36899.t1;Parent=MS.gene36899 |
| chr8.4 | exon | 63228436 | 63230121 | 63228436 | ID=MS.gene36899.t1.exon1;Parent=MS.gene36899.t1 |
| chr8.4 | CDS | 63228436 | 63230121 | 63228436 | ID=cds.MS.gene36899.t1;Parent=MS.gene36899.t1 |
| Gene Sequence |
| Protein sequence |
