Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71924.t1 | XP_003608748.1 | 97 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 2.20E-52 | 215.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71924.t1 | Q39176 | 69.6 | 168 | 50 | 1 | 1 | 168 | 1 | 167 | 8.6e-34 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71924.t1 | G7JJ02 | 97.0 | 169 | 5 | 0 | 1 | 169 | 1 | 169 | 1.6e-52 | 215.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene71924 | -0.804833 | 1.79E-49 | -1.69E-46 |
| MS.gene04932 | MS.gene71924 | 0.813591 | 2.37E-51 | -1.69E-46 |
| MS.gene049356 | MS.gene71924 | -0.816149 | 6.42E-52 | -1.69E-46 |
| MS.gene049488 | MS.gene71924 | -0.855412 | 6.42E-62 | -1.69E-46 |
| MS.gene049805 | MS.gene71924 | -0.808765 | 2.64E-50 | -1.69E-46 |
| MS.gene050060 | MS.gene71924 | -0.872279 | 3.59E-67 | -1.69E-46 |
| MS.gene050133 | MS.gene71924 | 0.809043 | 2.30E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene71924 | 0.874054 | 9.12E-68 | -1.69E-46 |
| MS.gene050464 | MS.gene71924 | -0.825996 | 3.46E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene71924 | -0.829847 | 4.09E-55 | -1.69E-46 |
| MS.gene050670 | MS.gene71924 | -0.86033 | 2.23E-63 | -1.69E-46 |
| MS.gene050710 | MS.gene71924 | 0.846594 | 1.97E-59 | -1.69E-46 |
| MS.gene050809 | MS.gene71924 | 0.82961 | 4.68E-55 | -1.69E-46 |
| MS.gene050971 | MS.gene71924 | -0.841545 | 4.45E-58 | -1.69E-46 |
| MS.gene05105 | MS.gene71924 | -0.800739 | 1.25E-48 | -1.69E-46 |
| MS.gene051158 | MS.gene71924 | 0.833256 | 5.92E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene71924 | -0.815611 | 8.46E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene71924 | 0.814044 | 1.88E-51 | -1.69E-46 |
| MS.gene051666 | MS.gene71924 | -0.804264 | 2.35E-49 | -1.69E-46 |
| MS.gene051838 | MS.gene71924 | -0.816176 | 6.33E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene71924 | 0.830042 | 3.67E-55 | -1.69E-46 |
| MS.gene052077 | MS.gene71924 | -0.820545 | 6.48E-53 | -1.69E-46 |
| MS.gene052128 | MS.gene71924 | 0.819109 | 1.38E-52 | -1.69E-46 |
| MS.gene052134 | MS.gene71924 | -0.813393 | 2.62E-51 | -1.69E-46 |
| MS.gene052135 | MS.gene71924 | -0.81556 | 8.69E-52 | -1.69E-46 |
| MS.gene052136 | MS.gene71924 | -0.805295 | 1.43E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene71924 | -0.855929 | 4.54E-62 | -1.69E-46 |
| MS.gene052223 | MS.gene71924 | 0.845185 | 4.75E-59 | -1.69E-46 |
| MS.gene052253 | MS.gene71924 | -0.81225 | 4.66E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene71924 | -0.807196 | 5.69E-50 | -1.69E-46 |
| MS.gene05233 | MS.gene71924 | 0.821725 | 3.47E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene71924 | -0.843687 | 1.20E-58 | -1.69E-46 |
| MS.gene05240 | MS.gene71924 | 0.821387 | 4.15E-53 | -1.69E-46 |
| MS.gene052414 | MS.gene71924 | -0.819881 | 9.20E-53 | -1.69E-46 |
| MS.gene052469 | MS.gene71924 | -0.842735 | 2.16E-58 | -1.69E-46 |
| MS.gene052702 | MS.gene71924 | 0.842598 | 2.35E-58 | -1.69E-46 |
| MS.gene052758 | MS.gene71924 | -0.829508 | 4.95E-55 | -1.69E-46 |
| MS.gene053449 | MS.gene71924 | -0.81165 | 6.30E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene71924 | -0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene053912 | MS.gene71924 | -0.84186 | 3.68E-58 | -1.69E-46 |
| MS.gene054107 | MS.gene71924 | -0.806115 | 9.62E-50 | -1.69E-46 |
| MS.gene054290 | MS.gene71924 | 0.84237 | 2.70E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene71924 | -0.831905 | 1.28E-55 | -1.69E-46 |
| MS.gene054742 | MS.gene71924 | 0.837051 | 6.53E-57 | -1.69E-46 |
| MS.gene054884 | MS.gene71924 | 0.832986 | 6.91E-56 | -1.69E-46 |
| MS.gene054955 | MS.gene71924 | -0.811271 | 7.61E-51 | -1.69E-46 |
| MS.gene055116 | MS.gene71924 | -0.806875 | 6.65E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene71924 | 0.835005 | 2.16E-56 | -1.69E-46 |
| MS.gene055159 | MS.gene71924 | 0.809611 | 1.74E-50 | -1.69E-46 |
| MS.gene055370 | MS.gene71924 | -0.808029 | 3.79E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene71924 | -0.848619 | 5.46E-60 | -1.69E-46 |
| MS.gene05553 | MS.gene71924 | -0.843809 | 1.11E-58 | -1.69E-46 |
| MS.gene05556 | MS.gene71924 | -0.812716 | 3.69E-51 | -1.69E-46 |
| MS.gene055601 | MS.gene71924 | 0.867928 | 9.53E-66 | -1.69E-46 |
| MS.gene055741 | MS.gene71924 | -0.803147 | 4.00E-49 | -1.69E-46 |
| MS.gene055744 | MS.gene71924 | -0.814247 | 1.70E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene71924 | -0.854612 | 1.10E-61 | -1.69E-46 |
| MS.gene056330 | MS.gene71924 | 0.838879 | 2.21E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene71924 | -0.839054 | 1.99E-57 | -1.69E-46 |
| MS.gene056514 | MS.gene71924 | -0.808856 | 2.52E-50 | -1.69E-46 |
| MS.gene056659 | MS.gene71924 | -0.833802 | 4.33E-56 | -1.69E-46 |
| MS.gene056688 | MS.gene71924 | 0.81577 | 7.80E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene71924 | 0.820726 | 5.89E-53 | -1.69E-46 |
| MS.gene056691 | MS.gene71924 | 0.827324 | 1.67E-54 | -1.69E-46 |
| MS.gene056697 | MS.gene71924 | 0.816533 | 5.27E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene71924 | 0.874258 | 7.77E-68 | -1.69E-46 |
| MS.gene057124 | MS.gene71924 | -0.841167 | 5.60E-58 | -1.69E-46 |
| MS.gene057356 | MS.gene71924 | 0.824929 | 6.18E-54 | -1.69E-46 |
| MS.gene057396 | MS.gene71924 | -0.83246 | 9.33E-56 | -1.69E-46 |
| MS.gene057544 | MS.gene71924 | 0.815088 | 1.11E-51 | -1.69E-46 |
| MS.gene057734 | MS.gene71924 | -0.810163 | 1.32E-50 | -1.69E-46 |
| MS.gene057749 | MS.gene71924 | -0.827319 | 1.67E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene71924 | -0.834186 | 3.47E-56 | -1.69E-46 |
| MS.gene058007 | MS.gene71924 | -0.824027 | 1.01E-53 | -1.69E-46 |
| MS.gene058158 | MS.gene71924 | 0.819219 | 1.30E-52 | -1.69E-46 |
| MS.gene058218 | MS.gene71924 | 0.823268 | 1.52E-53 | -1.69E-46 |
| MS.gene058376 | MS.gene71924 | -0.803609 | 3.21E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene71924 | -0.805847 | 1.10E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene71924 | -0.846137 | 2.62E-59 | -1.69E-46 |
| MS.gene059011 | MS.gene71924 | -0.803732 | 3.03E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene71924 | 0.800779 | 1.23E-48 | -1.69E-46 |
| MS.gene05926 | MS.gene71924 | 0.825744 | 3.97E-54 | -1.69E-46 |
| MS.gene059285 | MS.gene71924 | -0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene059366 | MS.gene71924 | -0.803685 | 3.10E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene71924 | 0.833354 | 5.60E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene71924 | -0.839344 | 1.68E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene71924 | -0.80745 | 5.03E-50 | -1.69E-46 |
| MS.gene059717 | MS.gene71924 | -0.836022 | 1.19E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene71924 | 0.816344 | 5.80E-52 | -1.69E-46 |
| MS.gene060247 | MS.gene71924 | -0.823244 | 1.54E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene71924 | -0.824309 | 8.66E-54 | -1.69E-46 |
| MS.gene060717 | MS.gene71924 | -0.800403 | 1.46E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene71924 | -0.800906 | 1.16E-48 | -1.69E-46 |
| MS.gene061051 | MS.gene71924 | 0.800481 | 1.41E-48 | -1.69E-46 |
| MS.gene061154 | MS.gene71924 | -0.81529 | 9.97E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene71924 | -0.810397 | 1.18E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71924.t1 | MTR_4g101330 | 96.450 | 169 | 6 | 0 | 1 | 169 | 1 | 169 | 1.10e-83 | 244 |
| MS.gene71924.t1 | MTR_4g101280 | 64.881 | 168 | 33 | 2 | 1 | 168 | 1 | 142 | 3.71e-59 | 181 |
| MS.gene71924.t1 | MTR_4g101310 | 72.093 | 86 | 24 | 0 | 83 | 168 | 48 | 133 | 8.43e-39 | 129 |
| MS.gene71924.t1 | MTR_4g101260 | 47.531 | 162 | 52 | 4 | 7 | 168 | 3 | 131 | 8.57e-37 | 124 |
| MS.gene71924.t1 | MTR_4g108150 | 46.296 | 162 | 45 | 4 | 7 | 168 | 3 | 122 | 1.33e-36 | 123 |
| MS.gene71924.t1 | MTR_1g012690 | 61.702 | 94 | 35 | 1 | 75 | 168 | 61 | 153 | 4.57e-33 | 115 |
| MS.gene71924.t1 | MTR_1g012700 | 54.386 | 114 | 49 | 2 | 57 | 168 | 51 | 163 | 7.27e-33 | 115 |
| MS.gene71924.t1 | MTR_1g012710 | 67.470 | 83 | 26 | 1 | 86 | 168 | 69 | 150 | 9.47e-33 | 114 |
| MS.gene71924.t1 | MTR_2g026760 | 42.857 | 154 | 49 | 3 | 8 | 161 | 6 | 120 | 2.82e-31 | 109 |
| MS.gene71924.t1 | MTR_3g106740 | 65.882 | 85 | 27 | 2 | 85 | 168 | 46 | 129 | 6.23e-31 | 108 |
| MS.gene71924.t1 | MTR_1g012630 | 65.060 | 83 | 28 | 1 | 86 | 168 | 128 | 209 | 1.87e-30 | 110 |
| MS.gene71924.t1 | MTR_5g012380 | 43.860 | 171 | 91 | 3 | 1 | 168 | 1 | 169 | 1.98e-30 | 109 |
| MS.gene71924.t1 | MTR_5g012340 | 42.135 | 178 | 91 | 4 | 1 | 168 | 1 | 176 | 8.69e-30 | 107 |
| MS.gene71924.t1 | MTR_3g106750 | 44.954 | 109 | 60 | 0 | 60 | 168 | 20 | 128 | 1.16e-22 | 87.8 |
| MS.gene71924.t1 | MTR_4g101270 | 50.000 | 84 | 41 | 1 | 85 | 168 | 104 | 186 | 3.25e-21 | 85.9 |
| MS.gene71924.t1 | MTR_2g026930 | 48.750 | 80 | 38 | 1 | 89 | 168 | 122 | 198 | 2.00e-16 | 73.6 |
| MS.gene71924.t1 | MTR_2g026920 | 48.750 | 80 | 38 | 1 | 89 | 168 | 118 | 194 | 2.02e-16 | 73.6 |
| MS.gene71924.t1 | MTR_2g026775 | 47.436 | 78 | 35 | 2 | 93 | 168 | 686 | 759 | 6.32e-15 | 72.0 |
| MS.gene71924.t1 | MTR_3g082100 | 45.977 | 87 | 46 | 1 | 82 | 168 | 250 | 335 | 4.52e-14 | 68.9 |
| MS.gene71924.t1 | MTR_2g026820 | 52.055 | 73 | 30 | 2 | 91 | 162 | 542 | 610 | 1.30e-13 | 68.2 |
| MS.gene71924.t1 | MTR_4g115360 | 47.619 | 84 | 43 | 1 | 85 | 168 | 150 | 232 | 2.30e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71924.t1 | AT4G12490 | 50.000 | 186 | 70 | 3 | 1 | 168 | 1 | 181 | 2.97e-46 | 149 |
| MS.gene71924.t1 | AT1G62510 | 82.759 | 87 | 15 | 0 | 82 | 168 | 63 | 149 | 7.77e-46 | 147 |
| MS.gene71924.t1 | AT4G12500 | 50.829 | 181 | 71 | 4 | 1 | 168 | 1 | 176 | 1.74e-45 | 147 |
| MS.gene71924.t1 | AT4G12520 | 51.786 | 168 | 41 | 2 | 1 | 168 | 1 | 128 | 1.78e-45 | 145 |
| MS.gene71924.t1 | AT4G12510 | 51.786 | 168 | 41 | 2 | 1 | 168 | 1 | 128 | 1.78e-45 | 145 |
| MS.gene71924.t1 | AT4G12480 | 50.595 | 168 | 82 | 1 | 1 | 168 | 1 | 167 | 4.72e-44 | 143 |
| MS.gene71924.t1 | AT4G12470 | 51.786 | 168 | 73 | 2 | 1 | 168 | 1 | 160 | 6.81e-40 | 132 |
| MS.gene71924.t1 | AT2G45180 | 70.238 | 84 | 24 | 1 | 85 | 168 | 51 | 133 | 5.69e-35 | 119 |
| MS.gene71924.t1 | AT4G12550 | 64.045 | 89 | 30 | 2 | 81 | 168 | 23 | 110 | 9.03e-34 | 115 |
| MS.gene71924.t1 | AT1G12100 | 42.945 | 163 | 54 | 2 | 7 | 168 | 7 | 131 | 1.78e-33 | 115 |
| MS.gene71924.t1 | AT4G22460 | 48.684 | 152 | 44 | 2 | 16 | 167 | 14 | 131 | 2.51e-33 | 115 |
| MS.gene71924.t1 | AT1G12090 | 57.031 | 128 | 25 | 1 | 41 | 168 | 40 | 137 | 9.41e-33 | 114 |
| MS.gene71924.t1 | AT4G12545 | 65.169 | 89 | 26 | 3 | 81 | 168 | 23 | 107 | 1.25e-30 | 107 |
| MS.gene71924.t1 | AT5G46890 | 43.827 | 162 | 52 | 4 | 7 | 168 | 4 | 126 | 3.21e-29 | 104 |
| MS.gene71924.t1 | AT5G46900 | 66.667 | 81 | 24 | 2 | 88 | 168 | 49 | 126 | 1.82e-28 | 102 |
| MS.gene71924.t1 | AT4G00165 | 48.182 | 110 | 53 | 3 | 60 | 168 | 22 | 128 | 1.31e-26 | 98.2 |
| MS.gene71924.t1 | AT4G00165 | 48.182 | 110 | 53 | 3 | 60 | 168 | 22 | 128 | 1.31e-26 | 98.2 |
| MS.gene71924.t1 | AT4G12530 | 57.143 | 84 | 35 | 1 | 85 | 168 | 33 | 115 | 1.19e-25 | 95.1 |
| MS.gene71924.t1 | AT2G10940 | 51.685 | 89 | 42 | 1 | 80 | 168 | 202 | 289 | 5.62e-22 | 90.1 |
| MS.gene71924.t1 | AT2G10940 | 51.685 | 89 | 42 | 1 | 80 | 168 | 202 | 289 | 5.62e-22 | 90.1 |
| MS.gene71924.t1 | AT3G22120 | 52.326 | 86 | 39 | 2 | 84 | 168 | 265 | 349 | 2.24e-20 | 86.7 |
| MS.gene71924.t1 | AT3G22120 | 52.326 | 86 | 39 | 2 | 84 | 168 | 248 | 332 | 2.89e-20 | 85.9 |
| MS.gene71924.t1 | AT3G22142 | 47.191 | 89 | 36 | 3 | 85 | 168 | 1396 | 1478 | 1.24e-19 | 85.5 |
| MS.gene71924.t1 | AT4G15160 | 48.235 | 85 | 43 | 1 | 85 | 168 | 98 | 182 | 1.65e-18 | 79.0 |
| MS.gene71924.t1 | AT4G15160 | 48.235 | 85 | 43 | 1 | 85 | 168 | 180 | 264 | 4.16e-18 | 79.3 |
| MS.gene71924.t1 | AT1G62500 | 45.238 | 84 | 45 | 1 | 85 | 168 | 211 | 293 | 1.06e-16 | 75.9 |
| MS.gene71924.t1 | AT4G22490 | 39.362 | 94 | 54 | 2 | 75 | 168 | 26 | 116 | 1.42e-14 | 66.6 |
Find 67 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCCAAAATGTTAGCTTTA+AGG | 0.143067 | 8.3:-19879518 | None:intergenic |
| TGCCCTTAAAGCTAACATTT+TGG | 0.186367 | 8.3:+19879515 | MS.gene71924:CDS |
| CTATGCTTTGGCTTCGGGTT+TGG | 0.225606 | 8.3:-19879216 | None:intergenic |
| TCAGGTGGATCTAGCCCTTC+AGG | 0.234581 | 8.3:+19879327 | MS.gene71924:CDS |
| TATGCTTTGGCTTCGGGTTT+GGG | 0.251072 | 8.3:-19879215 | None:intergenic |
| CAAGATCAACAAGGCCATTT+AGG | 0.261031 | 8.3:-19879470 | None:intergenic |
| TATGGTGGCTCGACCCCTTC+TGG | 0.277093 | 8.3:+19879291 | MS.gene71924:CDS |
| TTGGCCTATGCTTTGGCTTC+GGG | 0.278010 | 8.3:-19879221 | None:intergenic |
| CTTGGCCTATGCTTTGGCTT+CGG | 0.280439 | 8.3:-19879222 | None:intergenic |
| CCCCAAAATGTTAGCTTTAA+GGG | 0.288464 | 8.3:-19879517 | None:intergenic |
| CCATGCTGCTCCCTCCTAAA+TGG | 0.314462 | 8.3:+19879456 | MS.gene71924:CDS |
| AGGGAGCAGCATGGTGTTAC+TGG | 0.315372 | 8.3:-19879447 | None:intergenic |
| TCTGGAGGCTCGAGCCATTC+AGG | 0.343358 | 8.3:+19879255 | MS.gene71924:CDS |
| TTTCACTTGTCTCTTCTTGT+GGG | 0.345415 | 8.3:+19879172 | MS.gene71924:CDS |
| AGGCCAAGCCCAAACCATTC+TGG | 0.346045 | 8.3:+19879237 | MS.gene71924:CDS |
| TCGAGCCTCCAGAATGGTTT+GGG | 0.347685 | 8.3:-19879245 | None:intergenic |
| AGCAAGGGAAGAACAAGTTT+TGG | 0.351457 | 8.3:-19879121 | None:intergenic |
| CTCGAGCCTCCAGAATGGTT+TGG | 0.353959 | 8.3:-19879246 | None:intergenic |
| CCTCCAGAATGGTTTGGGCT+TGG | 0.371759 | 8.3:-19879240 | None:intergenic |
| GTATGTGCCAATGTACTAAA+TGG | 0.372850 | 8.3:+19879396 | MS.gene71924:CDS |
| AGAAGTAAGCTAAGTGAGAT+AGG | 0.373756 | 8.3:-19879555 | None:intergenic |
| TTTGGGCTTGGCCTATGCTT+TGG | 0.384486 | 8.3:-19879228 | None:intergenic |
| CTTCGGGTTTGGGCTAGGAC+TGG | 0.387569 | 8.3:-19879205 | None:intergenic |
| TTTGGCTTCGGGTTTGGGCT+AGG | 0.405141 | 8.3:-19879210 | None:intergenic |
| CAGGTGGATCTAGCCCTTCA+GGG | 0.408434 | 8.3:+19879328 | MS.gene71924:CDS |
| TCTGGAGGCTCAAGCCCCTC+AGG | 0.409707 | 8.3:+19879309 | MS.gene71924:CDS |
| TGGATTGTTGAATGTGACTT+TGG | 0.430067 | 8.3:+19879416 | MS.gene71924:CDS |
| GTAAGCTAAGTGAGATAGGA+AGG | 0.442604 | 8.3:-19879551 | None:intergenic |
| GTCTCTTCTTGTGGGACTTG+TGG | 0.449120 | 8.3:+19879180 | MS.gene71924:CDS |
| GGATTGTTGAATGTGACTTT+GGG | 0.453775 | 8.3:+19879417 | MS.gene71924:CDS |
| AGAAGGGGTCGAGCCACCAT+AGG | 0.474753 | 8.3:-19879289 | None:intergenic |
| CAACAATCCATTTAGTACAT+TGG | 0.483051 | 8.3:-19879403 | None:intergenic |
| GATGAATTAGTTAGTAGCAT+TGG | 0.486497 | 8.3:-19879611 | None:intergenic |
| TCAGGTGGATCTAGCCCCTA+TGG | 0.494479 | 8.3:+19879273 | MS.gene71924:CDS |
| AGGGGCTTGAGCCTCCAGAA+GGG | 0.499216 | 8.3:-19879305 | None:intergenic |
| GAAGGGGTCGAGCCACCATA+GGG | 0.502946 | 8.3:-19879288 | None:intergenic |
| CCACGTGATGCACTCAAACT+AGG | 0.524901 | 8.3:+19879372 | MS.gene71924:CDS |
| AAATGGCCTTGTTGATCTTG+AGG | 0.527575 | 8.3:+19879473 | MS.gene71924:CDS |
| GATCAACAAGGCCATTTAGG+AGG | 0.528124 | 8.3:-19879467 | None:intergenic |
| GGGGCTTGAGCCTCCAGAAG+GGG | 0.541979 | 8.3:-19879304 | None:intergenic |
| GGAGGCTCAAGCCCCTCAGG+TGG | 0.546342 | 8.3:+19879312 | MS.gene71924:CDS |
| GAGGGGCTTGAGCCTCCAGA+AGG | 0.546697 | 8.3:-19879306 | None:intergenic |
| AGAAGAGAACGTTGATTGTG+AGG | 0.556214 | 8.3:-19879149 | None:intergenic |
| CAAACCCGAAGCCAAAGCAT+AGG | 0.565887 | 8.3:+19879217 | MS.gene71924:CDS |
| AGGGGCTAGATCCACCTGAA+TGG | 0.566015 | 8.3:-19879269 | None:intergenic |
| AATGGCTCGAGCCTCCAGAA+TGG | 0.580527 | 8.3:-19879251 | None:intergenic |
| ATCAACAAGGCCATTTAGGA+GGG | 0.589716 | 8.3:-19879466 | None:intergenic |
| GAGTGCATCACGTGGGCACG+AGG | 0.603100 | 8.3:-19879364 | None:intergenic |
| TGATTGTGAGGAAAATAGCA+AGG | 0.608189 | 8.3:-19879137 | None:intergenic |
| GGAGGCTCGAGCCATTCAGG+TGG | 0.626870 | 8.3:+19879258 | MS.gene71924:CDS |
| AGGTGGATCTAGCCCTTCAG+GGG | 0.627938 | 8.3:+19879329 | MS.gene71924:CDS |
| GGTGGCTCGACCCCTTCTGG+AGG | 0.631941 | 8.3:+19879294 | MS.gene71924:CDS |
| GAAGGGCTAGATCCACCTGA+GGG | 0.641415 | 8.3:-19879324 | None:intergenic |
| TGAAGGGCTAGATCCACCTG+AGG | 0.647260 | 8.3:-19879325 | None:intergenic |
| GGTGGATCTAGCCCCTATGG+TGG | 0.647441 | 8.3:+19879276 | MS.gene71924:CDS |
| CCTAGTTTGAGTGCATCACG+TGG | 0.649545 | 8.3:-19879372 | None:intergenic |
| GAGCAGCATGGTGTTACTGG+TGG | 0.649599 | 8.3:-19879444 | None:intergenic |
| TGCATCACGTGGGCACGAGG+CGG | 0.652228 | 8.3:-19879361 | None:intergenic |
| CCAAGCCCAAACCATTCTGG+AGG | 0.666867 | 8.3:+19879240 | MS.gene71924:CDS |
| GATTGTGAGGAAAATAGCAA+GGG | 0.682608 | 8.3:-19879136 | None:intergenic |
| CAGCAGCCTCAAGATCAACA+AGG | 0.684442 | 8.3:-19879479 | None:intergenic |
| CCATTTAGGAGGGAGCAGCA+TGG | 0.695462 | 8.3:-19879456 | None:intergenic |
| AAGGGGTCGAGCCACCATAG+GGG | 0.715721 | 8.3:-19879287 | None:intergenic |
| AAGGGCTAGATCCACCTGAG+GGG | 0.722635 | 8.3:-19879323 | None:intergenic |
| TAGTAGCATTGGAAATCACG+TGG | 0.725861 | 8.3:-19879600 | None:intergenic |
| GGTGGATCTAGCCCTTCAGG+GGG | 0.733325 | 8.3:+19879330 | MS.gene71924:CDS |
| CTAGTTTGAGTGCATCACGT+GGG | 0.776308 | 8.3:-19879371 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAACAATCCATTTAGTACAT+TGG | - | chr8.3:19879406-19879425 | None:intergenic | 30.0% | |
| AGAAGTAAGCTAAGTGAGAT+AGG | - | chr8.3:19879558-19879577 | None:intergenic | 35.0% | |
| GATTGTGAGGAAAATAGCAA+GGG | - | chr8.3:19879139-19879158 | None:intergenic | 35.0% | |
| GGATTGTTGAATGTGACTTT+GGG | + | chr8.3:19879417-19879436 | MS.gene71924:CDS | 35.0% | |
| GTATGTGCCAATGTACTAAA+TGG | + | chr8.3:19879396-19879415 | MS.gene71924:CDS | 35.0% | |
| TGATTGTGAGGAAAATAGCA+AGG | - | chr8.3:19879140-19879159 | None:intergenic | 35.0% | |
| TGGATTGTTGAATGTGACTT+TGG | + | chr8.3:19879416-19879435 | MS.gene71924:CDS | 35.0% | |
| TTTCACTTGTCTCTTCTTGT+GGG | + | chr8.3:19879172-19879191 | MS.gene71924:CDS | 35.0% | |
| ! | CCCTTAAAGCTAACATTTTG+GGG | + | chr8.3:19879517-19879536 | MS.gene71924:CDS | 35.0% |
| ! | GCCCTTAAAGCTAACATTTT+GGG | + | chr8.3:19879516-19879535 | MS.gene71924:CDS | 35.0% |
| ! | TGCCCTTAAAGCTAACATTT+TGG | + | chr8.3:19879515-19879534 | MS.gene71924:CDS | 35.0% |
| ! | TTTTCACTTGTCTCTTCTTG+TGG | + | chr8.3:19879171-19879190 | MS.gene71924:CDS | 35.0% |
| !! | CCCCAAAATGTTAGCTTTAA+GGG | - | chr8.3:19879520-19879539 | None:intergenic | 35.0% |
| !! | TCCCCAAAATGTTAGCTTTA+AGG | - | chr8.3:19879521-19879540 | None:intergenic | 35.0% |
| ATCAACAAGGCCATTTAGGA+GGG | - | chr8.3:19879469-19879488 | None:intergenic | 40.0% | |
| CAAGATCAACAAGGCCATTT+AGG | - | chr8.3:19879473-19879492 | None:intergenic | 40.0% | |
| GTAAGCTAAGTGAGATAGGA+AGG | - | chr8.3:19879554-19879573 | None:intergenic | 40.0% | |
| ! | AGAAGAGAACGTTGATTGTG+AGG | - | chr8.3:19879152-19879171 | None:intergenic | 40.0% |
| ! | AGCAAGGGAAGAACAAGTTT+TGG | - | chr8.3:19879124-19879143 | None:intergenic | 40.0% |
| ! | TAGTAGCATTGGAAATCACG+TGG | - | chr8.3:19879603-19879622 | None:intergenic | 40.0% |
| !! | AAATGGCCTTGTTGATCTTG+AGG | + | chr8.3:19879473-19879492 | MS.gene71924:CDS | 40.0% |
| CTAGTTTGAGTGCATCACGT+GGG | - | chr8.3:19879374-19879393 | None:intergenic | 45.0% | |
| GATCAACAAGGCCATTTAGG+AGG | - | chr8.3:19879470-19879489 | None:intergenic | 45.0% | |
| ! | TATGCTTTGGCTTCGGGTTT+GGG | - | chr8.3:19879218-19879237 | None:intergenic | 45.0% |
| CAAACCCGAAGCCAAAGCAT+AGG | + | chr8.3:19879217-19879236 | MS.gene71924:CDS | 50.0% | |
| CAGCAGCCTCAAGATCAACA+AGG | - | chr8.3:19879482-19879501 | None:intergenic | 50.0% | |
| CCACGTGATGCACTCAAACT+AGG | + | chr8.3:19879372-19879391 | MS.gene71924:CDS | 50.0% | |
| CCTAGTTTGAGTGCATCACG+TGG | - | chr8.3:19879375-19879394 | None:intergenic | 50.0% | |
| GTCTCTTCTTGTGGGACTTG+TGG | + | chr8.3:19879180-19879199 | MS.gene71924:CDS | 50.0% | |
| TCGAGCCTCCAGAATGGTTT+GGG | - | chr8.3:19879248-19879267 | None:intergenic | 50.0% | |
| TTTGGGCTTGGCCTATGCTT+TGG | - | chr8.3:19879231-19879250 | None:intergenic | 50.0% | |
| ! | CTATGCTTTGGCTTCGGGTT+TGG | - | chr8.3:19879219-19879238 | None:intergenic | 50.0% |
| ! | CTTGGCCTATGCTTTGGCTT+CGG | - | chr8.3:19879225-19879244 | None:intergenic | 50.0% |
| ! | TTGGCCTATGCTTTGGCTTC+GGG | - | chr8.3:19879224-19879243 | None:intergenic | 50.0% |
| AAGGGCTAGATCCACCTGAG+GGG | - | chr8.3:19879326-19879345 | None:intergenic | 55.0% | |
| AATGGCTCGAGCCTCCAGAA+TGG | - | chr8.3:19879254-19879273 | None:intergenic | 55.0% | |
| AGGCCAAGCCCAAACCATTC+TGG | + | chr8.3:19879237-19879256 | MS.gene71924:CDS | 55.0% | |
| AGGGGCTAGATCCACCTGAA+TGG | - | chr8.3:19879272-19879291 | None:intergenic | 55.0% | |
| AGGTGGATCTAGCCCTTCAG+GGG | + | chr8.3:19879329-19879348 | MS.gene71924:CDS | 55.0% | |
| CAGGTGGATCTAGCCCTTCA+GGG | + | chr8.3:19879328-19879347 | MS.gene71924:CDS | 55.0% | |
| CCAAGCCCAAACCATTCTGG+AGG | + | chr8.3:19879240-19879259 | MS.gene71924:CDS | 55.0% | |
| CCATGCTGCTCCCTCCTAAA+TGG | + | chr8.3:19879456-19879475 | MS.gene71924:CDS | 55.0% | |
| CCATTTAGGAGGGAGCAGCA+TGG | - | chr8.3:19879459-19879478 | None:intergenic | 55.0% | |
| CTCGAGCCTCCAGAATGGTT+TGG | - | chr8.3:19879249-19879268 | None:intergenic | 55.0% | |
| GAAGGGCTAGATCCACCTGA+GGG | - | chr8.3:19879327-19879346 | None:intergenic | 55.0% | |
| TCAGGTGGATCTAGCCCCTA+TGG | + | chr8.3:19879273-19879292 | MS.gene71924:CDS | 55.0% | |
| TCAGGTGGATCTAGCCCTTC+AGG | + | chr8.3:19879327-19879346 | MS.gene71924:CDS | 55.0% | |
| TGAAGGGCTAGATCCACCTG+AGG | - | chr8.3:19879328-19879347 | None:intergenic | 55.0% | |
| ! | CCTCCAGAATGGTTTGGGCT+TGG | - | chr8.3:19879243-19879262 | None:intergenic | 55.0% |
| ! | TTTGGCTTCGGGTTTGGGCT+AGG | - | chr8.3:19879213-19879232 | None:intergenic | 55.0% |
| !! | AGGGAGCAGCATGGTGTTAC+TGG | - | chr8.3:19879450-19879469 | None:intergenic | 55.0% |
| !! | GAGCAGCATGGTGTTACTGG+TGG | - | chr8.3:19879447-19879466 | None:intergenic | 55.0% |
| AAGGGGTCGAGCCACCATAG+GGG | - | chr8.3:19879290-19879309 | None:intergenic | 60.0% | |
| AGAAGGGGTCGAGCCACCAT+AGG | - | chr8.3:19879292-19879311 | None:intergenic | 60.0% | |
| AGGGGCTTGAGCCTCCAGAA+GGG | - | chr8.3:19879308-19879327 | None:intergenic | 60.0% | |
| GAAGGGGTCGAGCCACCATA+GGG | - | chr8.3:19879291-19879310 | None:intergenic | 60.0% | |
| GGTGGATCTAGCCCCTATGG+TGG | + | chr8.3:19879276-19879295 | MS.gene71924:CDS | 60.0% | |
| GGTGGATCTAGCCCTTCAGG+GGG | + | chr8.3:19879330-19879349 | MS.gene71924:CDS | 60.0% | |
| TATGGTGGCTCGACCCCTTC+TGG | + | chr8.3:19879291-19879310 | MS.gene71924:CDS | 60.0% | |
| TCTAGCCCTTCAGGGGGAAG+TGG | + | chr8.3:19879336-19879355 | MS.gene71924:CDS | 60.0% | |
| TCTGGAGGCTCGAGCCATTC+AGG | + | chr8.3:19879255-19879274 | MS.gene71924:CDS | 60.0% | |
| ! | CTTCGGGTTTGGGCTAGGAC+TGG | - | chr8.3:19879208-19879227 | None:intergenic | 60.0% |
| GAGGGGCTTGAGCCTCCAGA+AGG | - | chr8.3:19879309-19879328 | None:intergenic | 65.0% | |
| GAGTGCATCACGTGGGCACG+AGG | - | chr8.3:19879367-19879386 | None:intergenic | 65.0% | |
| GGAGGCTCGAGCCATTCAGG+TGG | + | chr8.3:19879258-19879277 | MS.gene71924:CDS | 65.0% | |
| GGGGCTTGAGCCTCCAGAAG+GGG | - | chr8.3:19879307-19879326 | None:intergenic | 65.0% | |
| TCTGGAGGCTCAAGCCCCTC+AGG | + | chr8.3:19879309-19879328 | MS.gene71924:CDS | 65.0% | |
| TGCATCACGTGGGCACGAGG+CGG | - | chr8.3:19879364-19879383 | None:intergenic | 65.0% | |
| !! | CGGTGCCACTTCCCCCTGAA+GGG | - | chr8.3:19879344-19879363 | None:intergenic | 65.0% |
| GGAGGCTCAAGCCCCTCAGG+TGG | + | chr8.3:19879312-19879331 | MS.gene71924:CDS | 70.0% | |
| GGTGGCTCGACCCCTTCTGG+AGG | + | chr8.3:19879294-19879313 | MS.gene71924:CDS | 70.0% | |
| !! | GCGGTGCCACTTCCCCCTGA+AGG | - | chr8.3:19879345-19879364 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19879114 | 19879623 | 19879114 | ID=MS.gene71924 |
| chr8.3 | mRNA | 19879114 | 19879623 | 19879114 | ID=MS.gene71924.t1;Parent=MS.gene71924 |
| chr8.3 | exon | 19879114 | 19879623 | 19879114 | ID=MS.gene71924.t1.exon1;Parent=MS.gene71924.t1 |
| chr8.3 | CDS | 19879114 | 19879623 | 19879114 | ID=cds.MS.gene71924.t1;Parent=MS.gene71924.t1 |
| Gene Sequence |
| Protein sequence |
