Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62886.t1 | XP_003608741.1 | 100 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 3.20E-59 | 237.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62886.t1 | P14009 | 63.4 | 131 | 47 | 1 | 2 | 131 | 6 | 136 | 5.3e-31 | 135.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62886.t1 | G7JIZ5 | 100.0 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 2.3e-59 | 237.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene62886 | -0.82748 | 1.53E-54 | -1.69E-46 |
| MS.gene049150 | MS.gene62886 | -0.821442 | 4.03E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene62886 | -0.827558 | 1.46E-54 | -1.69E-46 |
| MS.gene04933 | MS.gene62886 | -0.809784 | 1.59E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene62886 | -0.808478 | 3.04E-50 | -1.69E-46 |
| MS.gene049993 | MS.gene62886 | -0.901689 | 2.08E-78 | -1.69E-46 |
| MS.gene050133 | MS.gene62886 | 0.808556 | 2.92E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene62886 | -0.808724 | 2.69E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene62886 | -0.846599 | 1.96E-59 | -1.69E-46 |
| MS.gene050406 | MS.gene62886 | -0.810064 | 1.39E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene62886 | 0.859745 | 3.34E-63 | -1.69E-46 |
| MS.gene050464 | MS.gene62886 | -0.87177 | 5.31E-67 | -1.69E-46 |
| MS.gene050529 | MS.gene62886 | -0.84449 | 7.32E-59 | -1.69E-46 |
| MS.gene050623 | MS.gene62886 | 0.80418 | 2.44E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene62886 | -0.852067 | 5.89E-61 | -1.69E-46 |
| MS.gene050670 | MS.gene62886 | -0.809243 | 2.08E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene62886 | -0.815562 | 8.67E-52 | -1.69E-46 |
| MS.gene051158 | MS.gene62886 | 0.836043 | 1.18E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene62886 | -0.836722 | 7.92E-57 | -1.69E-46 |
| MS.gene051860 | MS.gene62886 | -0.872166 | 3.92E-67 | -1.69E-46 |
| MS.gene051983 | MS.gene62886 | -0.862795 | 3.93E-64 | -1.69E-46 |
| MS.gene052011 | MS.gene62886 | 0.9042 | 1.57E-79 | -1.69E-46 |
| MS.gene052077 | MS.gene62886 | -0.851893 | 6.59E-61 | -1.69E-46 |
| MS.gene052128 | MS.gene62886 | 0.826288 | 2.94E-54 | -1.69E-46 |
| MS.gene052134 | MS.gene62886 | -0.813621 | 2.33E-51 | -1.69E-46 |
| MS.gene052135 | MS.gene62886 | -0.817893 | 2.60E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene62886 | -0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene62886 | 0.854633 | 1.08E-61 | -1.69E-46 |
| MS.gene052253 | MS.gene62886 | -0.85329 | 2.64E-61 | -1.69E-46 |
| MS.gene05233 | MS.gene62886 | 0.847636 | 1.02E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene62886 | -0.820466 | 6.76E-53 | -1.69E-46 |
| MS.gene053330 | MS.gene62886 | -0.817588 | 3.05E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene62886 | -0.806757 | 7.04E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene62886 | -0.857376 | 1.70E-62 | -1.69E-46 |
| MS.gene053971 | MS.gene62886 | -0.814306 | 1.65E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene62886 | -0.811364 | 7.27E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene62886 | -0.805745 | 1.15E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene62886 | -0.804013 | 2.65E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene62886 | -0.901937 | 1.62E-78 | -1.69E-46 |
| MS.gene054349 | MS.gene62886 | -0.847844 | 8.94E-60 | -1.69E-46 |
| MS.gene054473 | MS.gene62886 | -0.816531 | 5.27E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene62886 | -0.835834 | 1.33E-56 | -1.69E-46 |
| MS.gene054918 | MS.gene62886 | -0.836191 | 1.08E-56 | -1.69E-46 |
| MS.gene054983 | MS.gene62886 | -0.843342 | 1.49E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene62886 | -0.80815 | 3.57E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene62886 | 0.823718 | 1.19E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene62886 | -0.805762 | 1.14E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene62886 | -0.810355 | 1.20E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene62886 | -0.821416 | 4.08E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene62886 | -0.800365 | 1.49E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene62886 | -0.81321 | 2.87E-51 | -1.69E-46 |
| MS.gene055428 | MS.gene62886 | -0.80265 | 5.07E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene62886 | -0.843773 | 1.14E-58 | -1.69E-46 |
| MS.gene055601 | MS.gene62886 | 0.875512 | 2.91E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene62886 | -0.815723 | 7.99E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene62886 | -0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene056330 | MS.gene62886 | 0.837931 | 3.88E-57 | -1.69E-46 |
| MS.gene056337 | MS.gene62886 | -0.801727 | 7.86E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene62886 | -0.836793 | 7.60E-57 | -1.69E-46 |
| MS.gene056659 | MS.gene62886 | -0.815914 | 7.24E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene62886 | 0.822052 | 2.91E-53 | -1.69E-46 |
| MS.gene056691 | MS.gene62886 | 0.816522 | 5.29E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene62886 | -0.822199 | 2.69E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene62886 | 0.910098 | 2.74E-82 | -1.69E-46 |
| MS.gene056924 | MS.gene62886 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene62886 | -0.85934 | 4.42E-63 | -1.69E-46 |
| MS.gene05702 | MS.gene62886 | -0.82269 | 2.07E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene62886 | -0.813794 | 2.14E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene62886 | -0.815312 | 9.86E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene62886 | -0.805701 | 1.18E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene62886 | -0.84379 | 1.13E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene62886 | -0.819286 | 1.26E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene62886 | -0.810001 | 1.43E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene62886 | -0.809137 | 2.20E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene62886 | -0.831283 | 1.82E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene62886 | -0.82729 | 1.70E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene62886 | -0.846512 | 2.07E-59 | -1.69E-46 |
| MS.gene058218 | MS.gene62886 | 0.867719 | 1.11E-65 | -1.69E-46 |
| MS.gene058233 | MS.gene62886 | -0.801641 | 8.18E-49 | -1.69E-46 |
| MS.gene05856 | MS.gene62886 | -0.805721 | 1.16E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene62886 | -0.822093 | 2.85E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene62886 | -0.828048 | 1.12E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene62886 | -0.805364 | 1.38E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene62886 | -0.813941 | 1.98E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene62886 | 0.846377 | 2.26E-59 | -1.69E-46 |
| MS.gene05907 | MS.gene62886 | -0.848489 | 5.93E-60 | -1.69E-46 |
| MS.gene059247 | MS.gene62886 | -0.802932 | 4.44E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene62886 | -0.825404 | 4.78E-54 | -1.69E-46 |
| MS.gene059294 | MS.gene62886 | -0.826653 | 2.41E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene62886 | -0.878184 | 3.45E-69 | -1.69E-46 |
| MS.gene059462 | MS.gene62886 | -0.802541 | 5.34E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene62886 | -0.831116 | 2.00E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene62886 | -0.853132 | 2.92E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene62886 | -0.809132 | 2.20E-50 | -1.69E-46 |
| MS.gene060038 | MS.gene62886 | -0.811894 | 5.57E-51 | -1.69E-46 |
| MS.gene060247 | MS.gene62886 | -0.805116 | 1.56E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene62886 | -0.859747 | 3.34E-63 | -1.69E-46 |
| MS.gene061114 | MS.gene62886 | 0.863988 | 1.68E-64 | -1.69E-46 |
| MS.gene061154 | MS.gene62886 | -0.816299 | 5.94E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene62886 | -0.820614 | 6.25E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62886.t1 | MTR_4g101260 | 100.000 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 9.21e-92 | 261 |
| MS.gene62886.t1 | MTR_4g108150 | 90.152 | 132 | 4 | 1 | 1 | 132 | 1 | 123 | 4.38e-79 | 229 |
| MS.gene62886.t1 | MTR_2g026760 | 57.812 | 128 | 47 | 3 | 5 | 132 | 7 | 127 | 1.52e-39 | 129 |
| MS.gene62886.t1 | MTR_4g101280 | 54.745 | 137 | 55 | 2 | 3 | 132 | 7 | 143 | 2.16e-37 | 124 |
| MS.gene62886.t1 | MTR_4g101330 | 46.988 | 166 | 47 | 4 | 3 | 131 | 7 | 168 | 1.95e-36 | 122 |
| MS.gene62886.t1 | MTR_1g012690 | 61.856 | 97 | 35 | 1 | 36 | 132 | 60 | 154 | 2.69e-36 | 122 |
| MS.gene62886.t1 | MTR_1g012700 | 55.833 | 120 | 40 | 2 | 26 | 132 | 45 | 164 | 3.28e-36 | 122 |
| MS.gene62886.t1 | MTR_1g012710 | 65.060 | 83 | 29 | 0 | 50 | 132 | 69 | 151 | 2.55e-34 | 117 |
| MS.gene62886.t1 | MTR_1g012630 | 67.470 | 83 | 27 | 0 | 50 | 132 | 128 | 210 | 4.84e-34 | 118 |
| MS.gene62886.t1 | MTR_3g106740 | 69.880 | 83 | 24 | 1 | 50 | 131 | 47 | 129 | 6.21e-34 | 115 |
| MS.gene62886.t1 | MTR_4g101310 | 45.455 | 132 | 68 | 3 | 2 | 132 | 6 | 134 | 4.32e-29 | 103 |
| MS.gene62886.t1 | MTR_3g106750 | 61.905 | 84 | 31 | 1 | 50 | 132 | 46 | 129 | 2.02e-24 | 91.3 |
| MS.gene62886.t1 | MTR_5g012340 | 47.619 | 84 | 42 | 2 | 49 | 131 | 94 | 176 | 2.14e-23 | 89.7 |
| MS.gene62886.t1 | MTR_5g012380 | 47.619 | 84 | 42 | 2 | 49 | 131 | 87 | 169 | 6.00e-23 | 88.6 |
| MS.gene62886.t1 | MTR_4g101270 | 54.762 | 84 | 36 | 2 | 49 | 131 | 104 | 186 | 2.51e-21 | 84.7 |
| MS.gene62886.t1 | MTR_2g026930 | 45.570 | 79 | 41 | 1 | 53 | 131 | 122 | 198 | 5.41e-17 | 73.6 |
| MS.gene62886.t1 | MTR_2g026920 | 45.570 | 79 | 41 | 1 | 53 | 131 | 118 | 194 | 7.21e-17 | 73.2 |
| MS.gene62886.t1 | MTR_2g026775 | 46.053 | 76 | 36 | 2 | 59 | 132 | 688 | 760 | 6.52e-16 | 73.2 |
| MS.gene62886.t1 | MTR_2g026820 | 46.154 | 78 | 37 | 3 | 55 | 131 | 542 | 615 | 7.69e-13 | 64.3 |
| MS.gene62886.t1 | MTR_3g082100 | 42.857 | 84 | 46 | 2 | 49 | 131 | 253 | 335 | 1.52e-12 | 63.2 |
| MS.gene62886.t1 | MTR_4g115360 | 48.235 | 85 | 42 | 2 | 49 | 132 | 150 | 233 | 5.79e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62886.t1 | AT2G45180 | 61.029 | 136 | 47 | 2 | 1 | 132 | 1 | 134 | 3.38e-46 | 146 |
| MS.gene62886.t1 | AT5G46890 | 63.846 | 130 | 41 | 4 | 3 | 132 | 4 | 127 | 4.48e-41 | 133 |
| MS.gene62886.t1 | AT5G46900 | 62.308 | 130 | 43 | 3 | 3 | 132 | 4 | 127 | 5.56e-38 | 125 |
| MS.gene62886.t1 | AT4G12520 | 51.095 | 137 | 54 | 3 | 1 | 132 | 1 | 129 | 6.03e-38 | 125 |
| MS.gene62886.t1 | AT4G12510 | 51.095 | 137 | 54 | 3 | 1 | 132 | 1 | 129 | 6.03e-38 | 125 |
| MS.gene62886.t1 | AT1G62510 | 72.289 | 83 | 22 | 1 | 50 | 131 | 67 | 149 | 2.31e-36 | 122 |
| MS.gene62886.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 12 | 131 | 17 | 128 | 1.07e-34 | 117 |
| MS.gene62886.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 12 | 131 | 17 | 128 | 1.07e-34 | 117 |
| MS.gene62886.t1 | AT4G12480 | 41.071 | 168 | 63 | 3 | 1 | 132 | 1 | 168 | 1.89e-34 | 117 |
| MS.gene62886.t1 | AT1G12090 | 71.605 | 81 | 22 | 1 | 52 | 131 | 57 | 137 | 2.29e-33 | 114 |
| MS.gene62886.t1 | AT4G22460 | 58.095 | 105 | 40 | 3 | 26 | 129 | 29 | 130 | 2.83e-33 | 114 |
| MS.gene62886.t1 | AT4G12550 | 47.287 | 129 | 43 | 2 | 5 | 132 | 7 | 111 | 4.86e-33 | 112 |
| MS.gene62886.t1 | AT4G12490 | 62.105 | 95 | 33 | 2 | 39 | 132 | 90 | 182 | 1.38e-32 | 113 |
| MS.gene62886.t1 | AT4G12470 | 42.857 | 161 | 63 | 3 | 1 | 132 | 1 | 161 | 7.64e-32 | 110 |
| MS.gene62886.t1 | AT4G12500 | 63.095 | 84 | 30 | 1 | 50 | 132 | 94 | 177 | 8.61e-32 | 111 |
| MS.gene62886.t1 | AT4G12545 | 46.565 | 131 | 42 | 2 | 3 | 132 | 5 | 108 | 1.17e-31 | 108 |
| MS.gene62886.t1 | AT1G12100 | 45.312 | 128 | 64 | 3 | 6 | 132 | 10 | 132 | 4.26e-30 | 105 |
| MS.gene62886.t1 | AT4G12530 | 53.409 | 88 | 41 | 0 | 44 | 131 | 28 | 115 | 1.92e-27 | 98.6 |
| MS.gene62886.t1 | AT3G22142 | 52.381 | 84 | 38 | 2 | 49 | 131 | 1396 | 1478 | 2.72e-19 | 83.2 |
| MS.gene62886.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 49 | 132 | 207 | 290 | 7.43e-19 | 80.1 |
| MS.gene62886.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 49 | 132 | 207 | 290 | 7.43e-19 | 80.1 |
| MS.gene62886.t1 | AT1G62500 | 47.126 | 87 | 44 | 2 | 46 | 131 | 208 | 293 | 4.30e-18 | 78.2 |
| MS.gene62886.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 49 | 131 | 266 | 349 | 5.03e-18 | 78.6 |
| MS.gene62886.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 49 | 131 | 249 | 332 | 8.42e-18 | 77.8 |
| MS.gene62886.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 49 | 131 | 98 | 182 | 3.70e-17 | 73.9 |
| MS.gene62886.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 49 | 131 | 180 | 264 | 1.30e-16 | 73.9 |
| MS.gene62886.t1 | AT4G22490 | 35.165 | 91 | 53 | 2 | 43 | 131 | 30 | 116 | 2.99e-12 | 59.7 |
| MS.gene62886.t1 | AT4G22520 | 27.481 | 131 | 75 | 3 | 1 | 131 | 7 | 117 | 8.97e-12 | 58.2 |
Find 39 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACCAAGCTCAAGTTAA+TGG | 0.256664 | 8.3:+20014913 | None:intergenic |
| TCAAGTTAATGGGAAGATTA+AGG | 0.276650 | 8.3:+20014924 | None:intergenic |
| TGGTGTGTGTGCTGATGTAT+TGG | 0.312025 | 8.3:-20015074 | MS.gene62886:CDS |
| GTGCCTTTGCACTGCTCTTA+AGG | 0.336313 | 8.3:-20014966 | MS.gene62886:CDS |
| GGTGTGTGTGCTGATGTATT+GGG | 0.364523 | 8.3:-20015073 | MS.gene62886:CDS |
| CCATGCTGCTCTCTCATTGA+TGG | 0.378656 | 8.3:-20015013 | MS.gene62886:CDS |
| AGAGAGCAGCATGGTGTCTT+TGG | 0.413985 | 8.3:+20015022 | None:intergenic |
| CCTAGAGACACAATCAAGTT+TGG | 0.422527 | 8.3:-20015094 | MS.gene62886:CDS |
| TAAGCGCACACGAAACCCTT+TGG | 0.426755 | 8.3:+20014869 | None:intergenic |
| AGTACCAAGCTCAAGTTAAT+GGG | 0.431914 | 8.3:+20014914 | None:intergenic |
| TTGTGTCTCTAGGGCATGTT+GGG | 0.436629 | 8.3:+20015104 | None:intergenic |
| CGTGATCTTTGTGAGGTGGT+GGG | 0.442572 | 8.3:+20015164 | None:intergenic |
| CCGTGATCTTTGTGAGGTGG+TGG | 0.443706 | 8.3:+20015163 | None:intergenic |
| GTACTTAACTATTGTGGAAA+GGG | 0.444010 | 8.3:-20014896 | MS.gene62886:CDS |
| ATCATTCCAAATGGCTTCCA+AGG | 0.464713 | 8.3:-20015254 | None:intergenic |
| GAGCAGCATGGTGTCTTTGG+TGG | 0.477521 | 8.3:+20015025 | None:intergenic |
| ATTGTGTCTCTAGGGCATGT+TGG | 0.477867 | 8.3:+20015103 | None:intergenic |
| CCAAACTTGATTGTGTCTCT+AGG | 0.479073 | 8.3:+20015094 | None:intergenic |
| GTGATCTTTGTGAGGTGGTG+GGG | 0.487931 | 8.3:+20015165 | None:intergenic |
| GCTCTTAAGGCTAATGTCCT+AGG | 0.488278 | 8.3:-20014953 | MS.gene62886:CDS |
| TGGAGGAAGGTGGATGATGA+GGG | 0.490113 | 8.3:+20015131 | None:intergenic |
| TTGGAGGAAGGTGGATGATG+AGG | 0.490518 | 8.3:+20015130 | None:intergenic |
| TCTTCCCATTAACTTGAGCT+TGG | 0.493017 | 8.3:-20014918 | MS.gene62886:CDS |
| AGCTTGGTACTTAACTATTG+TGG | 0.494742 | 8.3:-20014902 | MS.gene62886:CDS |
| GCAAAGGAGCATAGCAACCT+TGG | 0.496091 | 8.3:+20015237 | None:intergenic |
| GGTTTGATTAATGTTGAGCT+TGG | 0.521860 | 8.3:-20015052 | MS.gene62886:CDS |
| TGATCTTTGTGAGGTGGTGG+GGG | 0.527911 | 8.3:+20015166 | None:intergenic |
| GGTACTTAACTATTGTGGAA+AGG | 0.533566 | 8.3:-20014897 | MS.gene62886:CDS |
| AAAATATGATGTTGAGGCAA+AGG | 0.535029 | 8.3:+20015221 | None:intergenic |
| GAAGATTAAGGTTAATGCCT+AGG | 0.539426 | 8.3:+20014936 | None:intergenic |
| CAAACTTGATTGTGTCTCTA+GGG | 0.541313 | 8.3:+20015095 | None:intergenic |
| TTGTGGAAAGGGAGTTCCAA+AGG | 0.553018 | 8.3:-20014885 | MS.gene62886:CDS |
| TGTGGAAAGGGAGTTCCAAA+GGG | 0.568343 | 8.3:-20014884 | MS.gene62886:CDS |
| CCACCACCTCACAAAGATCA+CGG | 0.588604 | 8.3:-20015163 | MS.gene62886:CDS |
| GTGGATGATGAGGGTGTGAG+TGG | 0.631843 | 8.3:+20015140 | None:intergenic |
| TAGCCTTAAGAGCAGTGCAA+AGG | 0.634750 | 8.3:+20014963 | None:intergenic |
| TGGCCGTGATCTTTGTGAGG+TGG | 0.666290 | 8.3:+20015160 | None:intergenic |
| CCATCAATGAGAGAGCAGCA+TGG | 0.704306 | 8.3:+20015013 | None:intergenic |
| GAGTGGCCGTGATCTTTGTG+AGG | 0.710036 | 8.3:+20015157 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATATGATGTTGAGGCAA+AGG | + | chr8.3:20014894-20014913 | None:intergenic | 30.0% | |
| CAGTGAAAAATATGATGTTG+AGG | + | chr8.3:20014900-20014919 | None:intergenic | 30.0% | |
| GTACTTAACTATTGTGGAAA+GGG | - | chr8.3:20015216-20015235 | MS.gene62886:CDS | 30.0% | |
| TCAAGTTAATGGGAAGATTA+AGG | + | chr8.3:20015191-20015210 | None:intergenic | 30.0% | |
| ! | CAACATCATATTTTTCACTG+TGG | - | chr8.3:20014900-20014919 | MS.gene62886:CDS | 30.0% |
| AAGTACCAAGCTCAAGTTAA+TGG | + | chr8.3:20015202-20015221 | None:intergenic | 35.0% | |
| AGCTTGGTACTTAACTATTG+TGG | - | chr8.3:20015210-20015229 | MS.gene62886:CDS | 35.0% | |
| AGTACCAAGCTCAAGTTAAT+GGG | + | chr8.3:20015201-20015220 | None:intergenic | 35.0% | |
| CAAACTTGATTGTGTCTCTA+GGG | + | chr8.3:20015020-20015039 | None:intergenic | 35.0% | |
| GAAGATTAAGGTTAATGCCT+AGG | + | chr8.3:20015179-20015198 | None:intergenic | 35.0% | |
| GGTACTTAACTATTGTGGAA+AGG | - | chr8.3:20015215-20015234 | MS.gene62886:CDS | 35.0% | |
| GGTTTGATTAATGTTGAGCT+TGG | - | chr8.3:20015060-20015079 | MS.gene62886:CDS | 35.0% | |
| CCAAACTTGATTGTGTCTCT+AGG | + | chr8.3:20015021-20015040 | None:intergenic | 40.0% | |
| CCTAGAGACACAATCAAGTT+TGG | - | chr8.3:20015018-20015037 | MS.gene62886:CDS | 40.0% | |
| ! | TCTTCCCATTAACTTGAGCT+TGG | - | chr8.3:20015194-20015213 | MS.gene62886:CDS | 40.0% |
| GCTCTTAAGGCTAATGTCCT+AGG | - | chr8.3:20015159-20015178 | MS.gene62886:CDS | 45.0% | |
| TGTGGAAAGGGAGTTCCAAA+GGG | - | chr8.3:20015228-20015247 | MS.gene62886:CDS | 45.0% | |
| TTGTGGAAAGGGAGTTCCAA+AGG | - | chr8.3:20015227-20015246 | MS.gene62886:CDS | 45.0% | |
| ! | GGTGTGTGTGCTGATGTATT+GGG | - | chr8.3:20015039-20015058 | MS.gene62886:CDS | 45.0% |
| ! | TAGCCTTAAGAGCAGTGCAA+AGG | + | chr8.3:20015152-20015171 | None:intergenic | 45.0% |
| ! | TGGTGTGTGTGCTGATGTAT+TGG | - | chr8.3:20015038-20015057 | MS.gene62886:CDS | 45.0% |
| !! | ATTGTGTCTCTAGGGCATGT+TGG | + | chr8.3:20015012-20015031 | None:intergenic | 45.0% |
| !! | CATGTTGGGTTTTTGGAGGA+AGG | + | chr8.3:20014997-20015016 | None:intergenic | 45.0% |
| !! | TCTAGGGCATGTTGGGTTTT+TGG | + | chr8.3:20015004-20015023 | None:intergenic | 45.0% |
| !! | TTGTGTCTCTAGGGCATGTT+GGG | + | chr8.3:20015011-20015030 | None:intergenic | 45.0% |
| AGAGAGCAGCATGGTGTCTT+TGG | + | chr8.3:20015093-20015112 | None:intergenic | 50.0% | |
| CCACCACCTCACAAAGATCA+CGG | - | chr8.3:20014949-20014968 | MS.gene62886:CDS | 50.0% | |
| CCATCAATGAGAGAGCAGCA+TGG | + | chr8.3:20015102-20015121 | None:intergenic | 50.0% | |
| CCATGCTGCTCTCTCATTGA+TGG | - | chr8.3:20015099-20015118 | MS.gene62886:CDS | 50.0% | |
| CGTGATCTTTGTGAGGTGGT+GGG | + | chr8.3:20014951-20014970 | None:intergenic | 50.0% | |
| GTGATCTTTGTGAGGTGGTG+GGG | + | chr8.3:20014950-20014969 | None:intergenic | 50.0% | |
| GTGCCTTTGCACTGCTCTTA+AGG | - | chr8.3:20015146-20015165 | MS.gene62886:CDS | 50.0% | |
| TAAGCGCACACGAAACCCTT+TGG | + | chr8.3:20015246-20015265 | None:intergenic | 50.0% | |
| TGGAGGAAGGTGGATGATGA+GGG | + | chr8.3:20014984-20015003 | None:intergenic | 50.0% | |
| TGGGGGACATGGAACATATG+TGG | + | chr8.3:20014932-20014951 | None:intergenic | 50.0% | |
| TTGGAGGAAGGTGGATGATG+AGG | + | chr8.3:20014985-20015004 | None:intergenic | 50.0% | |
| ! | GCAAAGGAGCATAGCAACCT+TGG | + | chr8.3:20014878-20014897 | None:intergenic | 50.0% |
| ! | TGATCTTTGTGAGGTGGTGG+GGG | + | chr8.3:20014949-20014968 | None:intergenic | 50.0% |
| !! | AGGGCATGTTGGGTTTTTGG+AGG | + | chr8.3:20015001-20015020 | None:intergenic | 50.0% |
| !! | GTTGGGTTTTTGGAGGAAGG+TGG | + | chr8.3:20014994-20015013 | None:intergenic | 50.0% |
| CCGTGATCTTTGTGAGGTGG+TGG | + | chr8.3:20014952-20014971 | None:intergenic | 55.0% | |
| GAGCAGCATGGTGTCTTTGG+TGG | + | chr8.3:20015090-20015109 | None:intergenic | 55.0% | |
| GAGTGGCCGTGATCTTTGTG+AGG | + | chr8.3:20014958-20014977 | None:intergenic | 55.0% | |
| TGGCCGTGATCTTTGTGAGG+TGG | + | chr8.3:20014955-20014974 | None:intergenic | 55.0% | |
| ! | GTGGATGATGAGGGTGTGAG+TGG | + | chr8.3:20014975-20014994 | None:intergenic | 55.0% |
| !! | TTGTGAGGTGGTGGGGGACA+TGG | + | chr8.3:20014943-20014962 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 20014868 | 20015266 | 20014868 | ID=MS.gene62886 |
| chr8.3 | mRNA | 20014868 | 20015266 | 20014868 | ID=MS.gene62886.t1;Parent=MS.gene62886 |
| chr8.3 | exon | 20014868 | 20015266 | 20014868 | ID=MS.gene62886.t1.exon1;Parent=MS.gene62886.t1 |
| chr8.3 | CDS | 20014868 | 20015266 | 20014868 | ID=cds.MS.gene62886.t1;Parent=MS.gene62886.t1 |
| Gene Sequence |
| Protein sequence |
