Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71927.t1 | XP_003608741.1 | 100 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 3.20E-59 | 237.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71927.t1 | P14009 | 63.4 | 131 | 47 | 1 | 2 | 131 | 6 | 136 | 5.3e-31 | 135.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71927.t1 | G7JIZ5 | 100.0 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 2.3e-59 | 237.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene71927 | -0.829922 | 3.92E-55 | -1.69E-46 |
| MS.gene049150 | MS.gene71927 | -0.818205 | 2.21E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene71927 | -0.823148 | 1.62E-53 | -1.69E-46 |
| MS.gene04933 | MS.gene71927 | -0.80329 | 3.74E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene71927 | -0.804422 | 2.18E-49 | -1.69E-46 |
| MS.gene049993 | MS.gene71927 | -0.906158 | 2.00E-80 | -1.69E-46 |
| MS.gene050133 | MS.gene71927 | 0.807938 | 3.96E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene71927 | -0.804714 | 1.89E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene71927 | -0.845547 | 3.79E-59 | -1.69E-46 |
| MS.gene050406 | MS.gene71927 | -0.806107 | 9.66E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene71927 | 0.86488 | 8.84E-65 | -1.69E-46 |
| MS.gene050464 | MS.gene71927 | -0.869753 | 2.44E-66 | -1.69E-46 |
| MS.gene050529 | MS.gene71927 | -0.843465 | 1.38E-58 | -1.69E-46 |
| MS.gene050623 | MS.gene71927 | 0.801671 | 8.07E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene71927 | -0.84855 | 5.71E-60 | -1.69E-46 |
| MS.gene050670 | MS.gene71927 | -0.806769 | 7.00E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene71927 | -0.811889 | 5.59E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene71927 | 0.837324 | 5.56E-57 | -1.69E-46 |
| MS.gene051525 | MS.gene71927 | -0.834012 | 3.83E-56 | -1.69E-46 |
| MS.gene051860 | MS.gene71927 | -0.867732 | 1.10E-65 | -1.69E-46 |
| MS.gene051983 | MS.gene71927 | -0.864867 | 8.93E-65 | -1.69E-46 |
| MS.gene052011 | MS.gene71927 | 0.902039 | 1.46E-78 | -1.69E-46 |
| MS.gene052077 | MS.gene71927 | -0.852272 | 5.15E-61 | -1.69E-46 |
| MS.gene052128 | MS.gene71927 | 0.822434 | 2.37E-53 | -1.69E-46 |
| MS.gene052134 | MS.gene71927 | -0.821662 | 3.58E-53 | -1.69E-46 |
| MS.gene052135 | MS.gene71927 | -0.826548 | 2.55E-54 | -1.69E-46 |
| MS.gene052223 | MS.gene71927 | 0.855694 | 5.31E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene71927 | -0.85175 | 7.24E-61 | -1.69E-46 |
| MS.gene05233 | MS.gene71927 | 0.844888 | 5.72E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene71927 | -0.818282 | 2.13E-52 | -1.69E-46 |
| MS.gene052758 | MS.gene71927 | -0.806538 | 7.84E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene71927 | -0.82224 | 2.63E-53 | -1.69E-46 |
| MS.gene053557 | MS.gene71927 | -0.801832 | 7.48E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene71927 | -0.853303 | 2.61E-61 | -1.69E-46 |
| MS.gene053971 | MS.gene71927 | -0.816979 | 4.18E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene71927 | -0.807164 | 5.78E-50 | -1.69E-46 |
| MS.gene054012 | MS.gene71927 | -0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene71927 | -0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene71927 | -0.90051 | 6.82E-78 | -1.69E-46 |
| MS.gene054349 | MS.gene71927 | -0.842823 | 2.04E-58 | -1.69E-46 |
| MS.gene054473 | MS.gene71927 | -0.816797 | 4.59E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene71927 | -0.83878 | 2.35E-57 | -1.69E-46 |
| MS.gene054918 | MS.gene71927 | -0.836711 | 7.97E-57 | -1.69E-46 |
| MS.gene054983 | MS.gene71927 | -0.844265 | 8.41E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene71927 | -0.804983 | 1.66E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene71927 | 0.823434 | 1.39E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene71927 | -0.809072 | 2.27E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene71927 | -0.805583 | 1.24E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene71927 | -0.818833 | 1.59E-52 | -1.69E-46 |
| MS.gene055369 | MS.gene71927 | -0.802482 | 5.50E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene71927 | -0.806621 | 7.53E-50 | -1.69E-46 |
| MS.gene05553 | MS.gene71927 | -0.845261 | 4.53E-59 | -1.69E-46 |
| MS.gene055601 | MS.gene71927 | 0.875852 | 2.22E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene71927 | -0.811057 | 8.47E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene71927 | -0.81315 | 2.96E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene71927 | 0.842921 | 1.92E-58 | -1.69E-46 |
| MS.gene056337 | MS.gene71927 | -0.802645 | 5.09E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene71927 | -0.837064 | 6.48E-57 | -1.69E-46 |
| MS.gene056659 | MS.gene71927 | -0.81869 | 1.72E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene71927 | 0.818816 | 1.61E-52 | -1.69E-46 |
| MS.gene056691 | MS.gene71927 | 0.817099 | 3.93E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene71927 | -0.819605 | 1.06E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene71927 | 0.912217 | 2.51E-83 | -1.69E-46 |
| MS.gene056979 | MS.gene71927 | -0.862212 | 5.95E-64 | -1.69E-46 |
| MS.gene05702 | MS.gene71927 | -0.824248 | 8.94E-54 | -1.69E-46 |
| MS.gene057121 | MS.gene71927 | -0.809393 | 1.93E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene71927 | -0.810016 | 1.42E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene71927 | -0.801595 | 8.36E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene71927 | -0.842759 | 2.12E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene71927 | -0.814498 | 1.49E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene71927 | -0.806414 | 8.32E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene71927 | -0.810447 | 1.15E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene71927 | -0.826092 | 3.28E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene71927 | -0.822036 | 2.94E-53 | -1.69E-46 |
| MS.gene058142 | MS.gene71927 | -0.848334 | 6.55E-60 | -1.69E-46 |
| MS.gene058218 | MS.gene71927 | 0.858121 | 1.02E-62 | -1.69E-46 |
| MS.gene05856 | MS.gene71927 | -0.802877 | 4.55E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene71927 | -0.820794 | 5.68E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene71927 | -0.825562 | 4.38E-54 | -1.69E-46 |
| MS.gene059011 | MS.gene71927 | -0.814407 | 1.56E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene71927 | 0.840804 | 6.97E-58 | -1.69E-46 |
| MS.gene05907 | MS.gene71927 | -0.843117 | 1.71E-58 | -1.69E-46 |
| MS.gene059247 | MS.gene71927 | -0.801239 | 9.89E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene71927 | -0.825825 | 3.79E-54 | -1.69E-46 |
| MS.gene059294 | MS.gene71927 | -0.820288 | 7.42E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene71927 | -0.87735 | 6.74E-69 | -1.69E-46 |
| MS.gene059462 | MS.gene71927 | -0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene71927 | -0.831949 | 1.25E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene71927 | -0.846494 | 2.10E-59 | -1.69E-46 |
| MS.gene059894 | MS.gene71927 | -0.806717 | 7.18E-50 | -1.69E-46 |
| MS.gene060038 | MS.gene71927 | -0.815088 | 1.11E-51 | -1.69E-46 |
| MS.gene060247 | MS.gene71927 | -0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene71927 | -0.855699 | 5.29E-62 | -1.69E-46 |
| MS.gene061114 | MS.gene71927 | 0.864843 | 9.08E-65 | -1.69E-46 |
| MS.gene061154 | MS.gene71927 | -0.813983 | 1.94E-51 | -1.69E-46 |
| MS.gene061159 | MS.gene71927 | -0.817491 | 3.21E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71927.t1 | MTR_4g101260 | 100.000 | 132 | 0 | 0 | 1 | 132 | 1 | 132 | 9.21e-92 | 261 |
| MS.gene71927.t1 | MTR_4g108150 | 90.152 | 132 | 4 | 1 | 1 | 132 | 1 | 123 | 4.38e-79 | 229 |
| MS.gene71927.t1 | MTR_2g026760 | 57.812 | 128 | 47 | 3 | 5 | 132 | 7 | 127 | 1.52e-39 | 129 |
| MS.gene71927.t1 | MTR_4g101280 | 54.745 | 137 | 55 | 2 | 3 | 132 | 7 | 143 | 2.16e-37 | 124 |
| MS.gene71927.t1 | MTR_4g101330 | 46.988 | 166 | 47 | 4 | 3 | 131 | 7 | 168 | 1.95e-36 | 122 |
| MS.gene71927.t1 | MTR_1g012690 | 61.856 | 97 | 35 | 1 | 36 | 132 | 60 | 154 | 2.69e-36 | 122 |
| MS.gene71927.t1 | MTR_1g012700 | 55.833 | 120 | 40 | 2 | 26 | 132 | 45 | 164 | 3.28e-36 | 122 |
| MS.gene71927.t1 | MTR_1g012710 | 65.060 | 83 | 29 | 0 | 50 | 132 | 69 | 151 | 2.55e-34 | 117 |
| MS.gene71927.t1 | MTR_1g012630 | 67.470 | 83 | 27 | 0 | 50 | 132 | 128 | 210 | 4.84e-34 | 118 |
| MS.gene71927.t1 | MTR_3g106740 | 69.880 | 83 | 24 | 1 | 50 | 131 | 47 | 129 | 6.21e-34 | 115 |
| MS.gene71927.t1 | MTR_4g101310 | 45.455 | 132 | 68 | 3 | 2 | 132 | 6 | 134 | 4.32e-29 | 103 |
| MS.gene71927.t1 | MTR_3g106750 | 61.905 | 84 | 31 | 1 | 50 | 132 | 46 | 129 | 2.02e-24 | 91.3 |
| MS.gene71927.t1 | MTR_5g012340 | 47.619 | 84 | 42 | 2 | 49 | 131 | 94 | 176 | 2.14e-23 | 89.7 |
| MS.gene71927.t1 | MTR_5g012380 | 47.619 | 84 | 42 | 2 | 49 | 131 | 87 | 169 | 6.00e-23 | 88.6 |
| MS.gene71927.t1 | MTR_4g101270 | 54.762 | 84 | 36 | 2 | 49 | 131 | 104 | 186 | 2.51e-21 | 84.7 |
| MS.gene71927.t1 | MTR_2g026930 | 45.570 | 79 | 41 | 1 | 53 | 131 | 122 | 198 | 5.41e-17 | 73.6 |
| MS.gene71927.t1 | MTR_2g026920 | 45.570 | 79 | 41 | 1 | 53 | 131 | 118 | 194 | 7.21e-17 | 73.2 |
| MS.gene71927.t1 | MTR_2g026775 | 46.053 | 76 | 36 | 2 | 59 | 132 | 688 | 760 | 6.52e-16 | 73.2 |
| MS.gene71927.t1 | MTR_2g026820 | 46.154 | 78 | 37 | 3 | 55 | 131 | 542 | 615 | 7.69e-13 | 64.3 |
| MS.gene71927.t1 | MTR_3g082100 | 42.857 | 84 | 46 | 2 | 49 | 131 | 253 | 335 | 1.52e-12 | 63.2 |
| MS.gene71927.t1 | MTR_4g115360 | 48.235 | 85 | 42 | 2 | 49 | 132 | 150 | 233 | 5.79e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71927.t1 | AT2G45180 | 61.029 | 136 | 47 | 2 | 1 | 132 | 1 | 134 | 3.38e-46 | 146 |
| MS.gene71927.t1 | AT5G46890 | 63.846 | 130 | 41 | 4 | 3 | 132 | 4 | 127 | 4.48e-41 | 133 |
| MS.gene71927.t1 | AT5G46900 | 62.308 | 130 | 43 | 3 | 3 | 132 | 4 | 127 | 5.56e-38 | 125 |
| MS.gene71927.t1 | AT4G12520 | 51.095 | 137 | 54 | 3 | 1 | 132 | 1 | 129 | 6.03e-38 | 125 |
| MS.gene71927.t1 | AT4G12510 | 51.095 | 137 | 54 | 3 | 1 | 132 | 1 | 129 | 6.03e-38 | 125 |
| MS.gene71927.t1 | AT1G62510 | 72.289 | 83 | 22 | 1 | 50 | 131 | 67 | 149 | 2.31e-36 | 122 |
| MS.gene71927.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 12 | 131 | 17 | 128 | 1.07e-34 | 117 |
| MS.gene71927.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 12 | 131 | 17 | 128 | 1.07e-34 | 117 |
| MS.gene71927.t1 | AT4G12480 | 41.071 | 168 | 63 | 3 | 1 | 132 | 1 | 168 | 1.89e-34 | 117 |
| MS.gene71927.t1 | AT1G12090 | 71.605 | 81 | 22 | 1 | 52 | 131 | 57 | 137 | 2.29e-33 | 114 |
| MS.gene71927.t1 | AT4G22460 | 58.095 | 105 | 40 | 3 | 26 | 129 | 29 | 130 | 2.83e-33 | 114 |
| MS.gene71927.t1 | AT4G12550 | 47.287 | 129 | 43 | 2 | 5 | 132 | 7 | 111 | 4.86e-33 | 112 |
| MS.gene71927.t1 | AT4G12490 | 62.105 | 95 | 33 | 2 | 39 | 132 | 90 | 182 | 1.38e-32 | 113 |
| MS.gene71927.t1 | AT4G12470 | 42.857 | 161 | 63 | 3 | 1 | 132 | 1 | 161 | 7.64e-32 | 110 |
| MS.gene71927.t1 | AT4G12500 | 63.095 | 84 | 30 | 1 | 50 | 132 | 94 | 177 | 8.61e-32 | 111 |
| MS.gene71927.t1 | AT4G12545 | 46.565 | 131 | 42 | 2 | 3 | 132 | 5 | 108 | 1.17e-31 | 108 |
| MS.gene71927.t1 | AT1G12100 | 45.312 | 128 | 64 | 3 | 6 | 132 | 10 | 132 | 4.26e-30 | 105 |
| MS.gene71927.t1 | AT4G12530 | 53.409 | 88 | 41 | 0 | 44 | 131 | 28 | 115 | 1.92e-27 | 98.6 |
| MS.gene71927.t1 | AT3G22142 | 52.381 | 84 | 38 | 2 | 49 | 131 | 1396 | 1478 | 2.72e-19 | 83.2 |
| MS.gene71927.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 49 | 132 | 207 | 290 | 7.43e-19 | 80.1 |
| MS.gene71927.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 49 | 132 | 207 | 290 | 7.43e-19 | 80.1 |
| MS.gene71927.t1 | AT1G62500 | 47.126 | 87 | 44 | 2 | 46 | 131 | 208 | 293 | 4.30e-18 | 78.2 |
| MS.gene71927.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 49 | 131 | 266 | 349 | 5.03e-18 | 78.6 |
| MS.gene71927.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 49 | 131 | 249 | 332 | 8.42e-18 | 77.8 |
| MS.gene71927.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 49 | 131 | 98 | 182 | 3.70e-17 | 73.9 |
| MS.gene71927.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 49 | 131 | 180 | 264 | 1.30e-16 | 73.9 |
| MS.gene71927.t1 | AT4G22490 | 35.165 | 91 | 53 | 2 | 43 | 131 | 30 | 116 | 2.99e-12 | 59.7 |
| MS.gene71927.t1 | AT4G22520 | 27.481 | 131 | 75 | 3 | 1 | 131 | 7 | 117 | 8.97e-12 | 58.2 |
Find 40 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACCAAGCTCAAGTTAA+TGG | 0.256664 | 8.3:+19911684 | None:intergenic |
| TCAAGTTAATGGGAAGATTA+AGG | 0.276650 | 8.3:+19911695 | None:intergenic |
| TGGTGTGTGTGCTGATGTAT+TGG | 0.312025 | 8.3:-19911845 | MS.gene71927:CDS |
| GTGCCTTTGCACTGCTCTTA+AGG | 0.336313 | 8.3:-19911737 | MS.gene71927:CDS |
| GGTGTGTGTGCTGATGTATT+GGG | 0.364523 | 8.3:-19911844 | MS.gene71927:CDS |
| CCATGCTGCTCTCTCATTGA+TGG | 0.378656 | 8.3:-19911784 | MS.gene71927:CDS |
| TAAGCACACACGAAACCCTT+TGG | 0.406388 | 8.3:+19911640 | None:intergenic |
| AGAGAGCAGCATGGTGTCTT+TGG | 0.413985 | 8.3:+19911793 | None:intergenic |
| CCTAGAGACACAATCAAGTT+TGG | 0.422527 | 8.3:-19911865 | MS.gene71927:CDS |
| AGTACCAAGCTCAAGTTAAT+GGG | 0.431914 | 8.3:+19911685 | None:intergenic |
| TTGTGTCTCTAGGGCATGTT+GGG | 0.436629 | 8.3:+19911875 | None:intergenic |
| CGTGATCTTTGTGAGGTGGT+GGG | 0.442572 | 8.3:+19911935 | None:intergenic |
| AAGCACACACGAAACCCTTT+GGG | 0.442916 | 8.3:+19911641 | None:intergenic |
| CCGTGATCTTTGTGAGGTGG+TGG | 0.443706 | 8.3:+19911934 | None:intergenic |
| GTACTTAACTATTGTGGAAA+GGG | 0.444010 | 8.3:-19911667 | MS.gene71927:CDS |
| ATCATTCCAAATGGCTTCCA+AGG | 0.464713 | 8.3:-19912025 | None:intergenic |
| GAGCAGCATGGTGTCTTTGG+TGG | 0.477521 | 8.3:+19911796 | None:intergenic |
| ATTGTGTCTCTAGGGCATGT+TGG | 0.477867 | 8.3:+19911874 | None:intergenic |
| CCAAACTTGATTGTGTCTCT+AGG | 0.479073 | 8.3:+19911865 | None:intergenic |
| GTGATCTTTGTGAGGTGGTG+GGG | 0.487931 | 8.3:+19911936 | None:intergenic |
| GCTCTTAAGGCTAATGTCCT+AGG | 0.488278 | 8.3:-19911724 | MS.gene71927:CDS |
| TGGAGGAAGGTGGATGATGA+GGG | 0.490113 | 8.3:+19911902 | None:intergenic |
| TTGGAGGAAGGTGGATGATG+AGG | 0.490518 | 8.3:+19911901 | None:intergenic |
| TCTTCCCATTAACTTGAGCT+TGG | 0.493017 | 8.3:-19911689 | MS.gene71927:CDS |
| AGCTTGGTACTTAACTATTG+TGG | 0.494742 | 8.3:-19911673 | MS.gene71927:CDS |
| GCAAAGGAGCATAGCAACCT+TGG | 0.496091 | 8.3:+19912008 | None:intergenic |
| GGTTTGATTAATGTTGAGCT+TGG | 0.521860 | 8.3:-19911823 | MS.gene71927:CDS |
| TGATCTTTGTGAGGTGGTGG+GGG | 0.527911 | 8.3:+19911937 | None:intergenic |
| GGTACTTAACTATTGTGGAA+AGG | 0.533566 | 8.3:-19911668 | MS.gene71927:CDS |
| AAAATATGATGTTGAGGCAA+AGG | 0.535029 | 8.3:+19911992 | None:intergenic |
| GAAGATTAAGGTTAATGCCT+AGG | 0.539426 | 8.3:+19911707 | None:intergenic |
| CAAACTTGATTGTGTCTCTA+GGG | 0.541313 | 8.3:+19911866 | None:intergenic |
| TGTGGAAAGGGAGTCCCAAA+GGG | 0.569713 | 8.3:-19911655 | MS.gene71927:CDS |
| TTGTGGAAAGGGAGTCCCAA+AGG | 0.585738 | 8.3:-19911656 | MS.gene71927:CDS |
| CCACCACCTCACAAAGATCA+CGG | 0.588604 | 8.3:-19911934 | MS.gene71927:CDS |
| GTGGATGATGAGGGTGTGAG+TGG | 0.631843 | 8.3:+19911911 | None:intergenic |
| TAGCCTTAAGAGCAGTGCAA+AGG | 0.634750 | 8.3:+19911734 | None:intergenic |
| TGGCCGTGATCTTTGTGAGG+TGG | 0.666290 | 8.3:+19911931 | None:intergenic |
| CCATCAATGAGAGAGCAGCA+TGG | 0.704306 | 8.3:+19911784 | None:intergenic |
| GAGTGGCCGTGATCTTTGTG+AGG | 0.710036 | 8.3:+19911928 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATATGATGTTGAGGCAA+AGG | + | chr8.3:19911665-19911684 | None:intergenic | 30.0% | |
| CAGTGAAAAATATGATGTTG+AGG | + | chr8.3:19911671-19911690 | None:intergenic | 30.0% | |
| GTACTTAACTATTGTGGAAA+GGG | - | chr8.3:19911987-19912006 | MS.gene71927:CDS | 30.0% | |
| TCAAGTTAATGGGAAGATTA+AGG | + | chr8.3:19911962-19911981 | None:intergenic | 30.0% | |
| ! | CAACATCATATTTTTCACTG+TGG | - | chr8.3:19911671-19911690 | MS.gene71927:CDS | 30.0% |
| AAGTACCAAGCTCAAGTTAA+TGG | + | chr8.3:19911973-19911992 | None:intergenic | 35.0% | |
| AGCTTGGTACTTAACTATTG+TGG | - | chr8.3:19911981-19912000 | MS.gene71927:CDS | 35.0% | |
| AGTACCAAGCTCAAGTTAAT+GGG | + | chr8.3:19911972-19911991 | None:intergenic | 35.0% | |
| CAAACTTGATTGTGTCTCTA+GGG | + | chr8.3:19911791-19911810 | None:intergenic | 35.0% | |
| GAAGATTAAGGTTAATGCCT+AGG | + | chr8.3:19911950-19911969 | None:intergenic | 35.0% | |
| GGTACTTAACTATTGTGGAA+AGG | - | chr8.3:19911986-19912005 | MS.gene71927:CDS | 35.0% | |
| GGTTTGATTAATGTTGAGCT+TGG | - | chr8.3:19911831-19911850 | MS.gene71927:CDS | 35.0% | |
| CCAAACTTGATTGTGTCTCT+AGG | + | chr8.3:19911792-19911811 | None:intergenic | 40.0% | |
| CCTAGAGACACAATCAAGTT+TGG | - | chr8.3:19911789-19911808 | MS.gene71927:CDS | 40.0% | |
| ! | TCTTCCCATTAACTTGAGCT+TGG | - | chr8.3:19911965-19911984 | MS.gene71927:CDS | 40.0% |
| AAGCACACACGAAACCCTTT+GGG | + | chr8.3:19912016-19912035 | None:intergenic | 45.0% | |
| GCTCTTAAGGCTAATGTCCT+AGG | - | chr8.3:19911930-19911949 | MS.gene71927:CDS | 45.0% | |
| TAAGCACACACGAAACCCTT+TGG | + | chr8.3:19912017-19912036 | None:intergenic | 45.0% | |
| ! | GGTGTGTGTGCTGATGTATT+GGG | - | chr8.3:19911810-19911829 | MS.gene71927:CDS | 45.0% |
| ! | TAGCCTTAAGAGCAGTGCAA+AGG | + | chr8.3:19911923-19911942 | None:intergenic | 45.0% |
| ! | TGGTGTGTGTGCTGATGTAT+TGG | - | chr8.3:19911809-19911828 | MS.gene71927:CDS | 45.0% |
| !! | ATTGTGTCTCTAGGGCATGT+TGG | + | chr8.3:19911783-19911802 | None:intergenic | 45.0% |
| !! | CATGTTGGGTTTTTGGAGGA+AGG | + | chr8.3:19911768-19911787 | None:intergenic | 45.0% |
| !! | TCTAGGGCATGTTGGGTTTT+TGG | + | chr8.3:19911775-19911794 | None:intergenic | 45.0% |
| !! | TTGTGTCTCTAGGGCATGTT+GGG | + | chr8.3:19911782-19911801 | None:intergenic | 45.0% |
| AGAGAGCAGCATGGTGTCTT+TGG | + | chr8.3:19911864-19911883 | None:intergenic | 50.0% | |
| CCACCACCTCACAAAGATCA+CGG | - | chr8.3:19911720-19911739 | MS.gene71927:CDS | 50.0% | |
| CCATCAATGAGAGAGCAGCA+TGG | + | chr8.3:19911873-19911892 | None:intergenic | 50.0% | |
| CCATGCTGCTCTCTCATTGA+TGG | - | chr8.3:19911870-19911889 | MS.gene71927:CDS | 50.0% | |
| CGTGATCTTTGTGAGGTGGT+GGG | + | chr8.3:19911722-19911741 | None:intergenic | 50.0% | |
| GTGATCTTTGTGAGGTGGTG+GGG | + | chr8.3:19911721-19911740 | None:intergenic | 50.0% | |
| GTGCCTTTGCACTGCTCTTA+AGG | - | chr8.3:19911917-19911936 | MS.gene71927:CDS | 50.0% | |
| TGGAGGAAGGTGGATGATGA+GGG | + | chr8.3:19911755-19911774 | None:intergenic | 50.0% | |
| TGGGGGACATGGAACATATG+TGG | + | chr8.3:19911703-19911722 | None:intergenic | 50.0% | |
| TGTGGAAAGGGAGTCCCAAA+GGG | - | chr8.3:19911999-19912018 | MS.gene71927:CDS | 50.0% | |
| TTGGAGGAAGGTGGATGATG+AGG | + | chr8.3:19911756-19911775 | None:intergenic | 50.0% | |
| TTGTGGAAAGGGAGTCCCAA+AGG | - | chr8.3:19911998-19912017 | MS.gene71927:CDS | 50.0% | |
| ! | GCAAAGGAGCATAGCAACCT+TGG | + | chr8.3:19911649-19911668 | None:intergenic | 50.0% |
| ! | TGATCTTTGTGAGGTGGTGG+GGG | + | chr8.3:19911720-19911739 | None:intergenic | 50.0% |
| !! | AGGGCATGTTGGGTTTTTGG+AGG | + | chr8.3:19911772-19911791 | None:intergenic | 50.0% |
| !! | GTTGGGTTTTTGGAGGAAGG+TGG | + | chr8.3:19911765-19911784 | None:intergenic | 50.0% |
| CCGTGATCTTTGTGAGGTGG+TGG | + | chr8.3:19911723-19911742 | None:intergenic | 55.0% | |
| GAGCAGCATGGTGTCTTTGG+TGG | + | chr8.3:19911861-19911880 | None:intergenic | 55.0% | |
| GAGTGGCCGTGATCTTTGTG+AGG | + | chr8.3:19911729-19911748 | None:intergenic | 55.0% | |
| TGGCCGTGATCTTTGTGAGG+TGG | + | chr8.3:19911726-19911745 | None:intergenic | 55.0% | |
| ! | GTGGATGATGAGGGTGTGAG+TGG | + | chr8.3:19911746-19911765 | None:intergenic | 55.0% |
| !! | TTGTGAGGTGGTGGGGGACA+TGG | + | chr8.3:19911714-19911733 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19911639 | 19912037 | 19911639 | ID=MS.gene71927 |
| chr8.3 | mRNA | 19911639 | 19912037 | 19911639 | ID=MS.gene71927.t1;Parent=MS.gene71927 |
| chr8.3 | exon | 19911639 | 19912037 | 19911639 | ID=MS.gene71927.t1.exon1;Parent=MS.gene71927.t1 |
| chr8.3 | CDS | 19911639 | 19912037 | 19911639 | ID=cds.MS.gene71927.t1;Parent=MS.gene71927.t1 |
| Gene Sequence |
| Protein sequence |
