Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62885.t1 | XP_003608741.1 | 100 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 4.90E-57 | 230.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62885.t1 | P14009 | 64.8 | 128 | 44 | 1 | 1 | 127 | 9 | 136 | 1.2e-30 | 134.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62885.t1 | G7JIZ5 | 100.0 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 3.5e-57 | 230.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene62885 | -0.8296 | 4.70E-55 | -1.69E-46 |
| MS.gene049150 | MS.gene62885 | -0.821355 | 4.22E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene62885 | -0.8274 | 1.60E-54 | -1.69E-46 |
| MS.gene04933 | MS.gene62885 | -0.807389 | 5.18E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene62885 | -0.809451 | 1.88E-50 | -1.69E-46 |
| MS.gene049993 | MS.gene62885 | -0.901239 | 3.28E-78 | -1.69E-46 |
| MS.gene050133 | MS.gene62885 | 0.808307 | 3.30E-50 | -1.69E-46 |
| MS.gene050301 | MS.gene62885 | -0.808793 | 2.60E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene62885 | -0.846102 | 2.68E-59 | -1.69E-46 |
| MS.gene050406 | MS.gene62885 | -0.810163 | 1.32E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene62885 | 0.860415 | 2.10E-63 | -1.69E-46 |
| MS.gene050464 | MS.gene62885 | -0.872867 | 2.29E-67 | -1.69E-46 |
| MS.gene050529 | MS.gene62885 | -0.845374 | 4.23E-59 | -1.69E-46 |
| MS.gene050623 | MS.gene62885 | 0.804612 | 1.99E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene62885 | -0.85186 | 6.74E-61 | -1.69E-46 |
| MS.gene050670 | MS.gene62885 | -0.809947 | 1.47E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene62885 | -0.815772 | 7.79E-52 | -1.69E-46 |
| MS.gene051158 | MS.gene62885 | 0.836006 | 1.20E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene62885 | -0.836317 | 1.00E-56 | -1.69E-46 |
| MS.gene051860 | MS.gene62885 | -0.87116 | 8.44E-67 | -1.69E-46 |
| MS.gene051983 | MS.gene62885 | -0.864033 | 1.63E-64 | -1.69E-46 |
| MS.gene052011 | MS.gene62885 | 0.902748 | 7.06E-79 | -1.69E-46 |
| MS.gene052077 | MS.gene62885 | -0.852867 | 3.48E-61 | -1.69E-46 |
| MS.gene052128 | MS.gene62885 | 0.826686 | 2.37E-54 | -1.69E-46 |
| MS.gene052134 | MS.gene62885 | -0.81582 | 7.60E-52 | -1.69E-46 |
| MS.gene052135 | MS.gene62885 | -0.819903 | 9.10E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene62885 | -0.803573 | 3.27E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene62885 | 0.855019 | 8.35E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene62885 | -0.852843 | 3.54E-61 | -1.69E-46 |
| MS.gene05233 | MS.gene62885 | 0.84774 | 9.55E-60 | -1.69E-46 |
| MS.gene052340 | MS.gene62885 | -0.82222 | 2.66E-53 | -1.69E-46 |
| MS.gene052758 | MS.gene62885 | -0.801408 | 9.13E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene62885 | -0.81753 | 3.14E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene62885 | -0.807985 | 3.87E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene62885 | -0.857481 | 1.59E-62 | -1.69E-46 |
| MS.gene053971 | MS.gene62885 | -0.815167 | 1.06E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene62885 | -0.810235 | 1.27E-50 | -1.69E-46 |
| MS.gene054012 | MS.gene62885 | -0.804336 | 2.27E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene62885 | -0.805074 | 1.59E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene62885 | -0.900856 | 4.82E-78 | -1.69E-46 |
| MS.gene054349 | MS.gene62885 | -0.848237 | 6.96E-60 | -1.69E-46 |
| MS.gene054473 | MS.gene62885 | -0.816458 | 5.47E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene62885 | -0.836284 | 1.02E-56 | -1.69E-46 |
| MS.gene054918 | MS.gene62885 | -0.836318 | 1.00E-56 | -1.69E-46 |
| MS.gene054983 | MS.gene62885 | -0.843717 | 1.18E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene62885 | -0.808907 | 2.46E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene62885 | 0.82368 | 1.22E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene62885 | -0.804907 | 1.72E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene62885 | -0.810572 | 1.08E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene62885 | -0.821275 | 4.40E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene62885 | -0.801426 | 9.05E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene62885 | -0.813241 | 2.83E-51 | -1.69E-46 |
| MS.gene055428 | MS.gene62885 | -0.802784 | 4.76E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene62885 | -0.844385 | 7.81E-59 | -1.69E-46 |
| MS.gene055601 | MS.gene62885 | 0.876043 | 1.91E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene62885 | -0.815693 | 8.11E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene62885 | -0.816495 | 5.37E-52 | -1.69E-46 |
| MS.gene056330 | MS.gene62885 | 0.838687 | 2.48E-57 | -1.69E-46 |
| MS.gene056337 | MS.gene62885 | -0.802174 | 6.36E-49 | -1.69E-46 |
| MS.gene056612 | MS.gene62885 | -0.837142 | 6.19E-57 | -1.69E-46 |
| MS.gene056659 | MS.gene62885 | -0.816439 | 5.53E-52 | -1.69E-46 |
| MS.gene056690 | MS.gene62885 | 0.820914 | 5.33E-53 | -1.69E-46 |
| MS.gene056691 | MS.gene62885 | 0.814699 | 1.35E-51 | -1.69E-46 |
| MS.gene056723 | MS.gene62885 | -0.821859 | 3.23E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene62885 | 0.911277 | 7.30E-83 | -1.69E-46 |
| MS.gene056924 | MS.gene62885 | 0.80068 | 1.29E-48 | -1.69E-46 |
| MS.gene056979 | MS.gene62885 | -0.860536 | 1.93E-63 | -1.69E-46 |
| MS.gene05702 | MS.gene62885 | -0.823001 | 1.75E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene62885 | -0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene62885 | -0.814419 | 1.56E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene62885 | -0.805061 | 1.60E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene62885 | -0.842362 | 2.71E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene62885 | -0.819302 | 1.25E-52 | -1.69E-46 |
| MS.gene057823 | MS.gene62885 | -0.809908 | 1.50E-50 | -1.69E-46 |
| MS.gene057888 | MS.gene62885 | -0.80799 | 3.86E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene62885 | -0.831989 | 1.22E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene62885 | -0.827105 | 1.88E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene62885 | -0.847935 | 8.44E-60 | -1.69E-46 |
| MS.gene058218 | MS.gene62885 | 0.867388 | 1.42E-65 | -1.69E-46 |
| MS.gene058233 | MS.gene62885 | -0.801484 | 8.81E-49 | -1.69E-46 |
| MS.gene05856 | MS.gene62885 | -0.806647 | 7.43E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene62885 | -0.822204 | 2.68E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene62885 | -0.82731 | 1.68E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene62885 | -0.805808 | 1.12E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene62885 | -0.813993 | 1.93E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene62885 | 0.845441 | 4.05E-59 | -1.69E-46 |
| MS.gene05907 | MS.gene62885 | -0.848061 | 7.79E-60 | -1.69E-46 |
| MS.gene059247 | MS.gene62885 | -0.803086 | 4.12E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene62885 | -0.827488 | 1.52E-54 | -1.69E-46 |
| MS.gene059294 | MS.gene62885 | -0.825895 | 3.65E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene62885 | -0.878147 | 3.55E-69 | -1.69E-46 |
| MS.gene059462 | MS.gene62885 | -0.801894 | 7.26E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene62885 | -0.800668 | 1.29E-48 | -1.69E-46 |
| MS.gene059609 | MS.gene62885 | -0.831125 | 1.99E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene62885 | -0.85323 | 2.74E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene62885 | -0.810127 | 1.35E-50 | -1.69E-46 |
| MS.gene060038 | MS.gene62885 | -0.811063 | 8.44E-51 | -1.69E-46 |
| MS.gene060247 | MS.gene62885 | -0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene62885 | -0.859954 | 2.89E-63 | -1.69E-46 |
| MS.gene061114 | MS.gene62885 | 0.863311 | 2.72E-64 | -1.69E-46 |
| MS.gene061154 | MS.gene62885 | -0.817304 | 3.53E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene62885 | -0.820541 | 6.50E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62885.t1 | MTR_4g101260 | 100.000 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 1.16e-88 | 253 |
| MS.gene62885.t1 | MTR_4g108150 | 89.844 | 128 | 4 | 1 | 1 | 128 | 5 | 123 | 5.31e-76 | 221 |
| MS.gene62885.t1 | MTR_2g026760 | 57.812 | 128 | 47 | 3 | 1 | 128 | 7 | 127 | 1.82e-39 | 129 |
| MS.gene62885.t1 | MTR_4g101280 | 54.815 | 135 | 54 | 2 | 1 | 128 | 9 | 143 | 8.84e-37 | 122 |
| MS.gene62885.t1 | MTR_1g012690 | 61.856 | 97 | 35 | 1 | 32 | 128 | 60 | 154 | 2.85e-36 | 121 |
| MS.gene62885.t1 | MTR_1g012700 | 55.833 | 120 | 40 | 2 | 22 | 128 | 45 | 164 | 4.14e-36 | 121 |
| MS.gene62885.t1 | MTR_4g101330 | 46.951 | 164 | 46 | 4 | 1 | 127 | 9 | 168 | 7.69e-36 | 121 |
| MS.gene62885.t1 | MTR_1g012710 | 65.060 | 83 | 29 | 0 | 46 | 128 | 69 | 151 | 2.74e-34 | 116 |
| MS.gene62885.t1 | MTR_1g012630 | 67.470 | 83 | 27 | 0 | 46 | 128 | 128 | 210 | 5.48e-34 | 117 |
| MS.gene62885.t1 | MTR_3g106740 | 69.880 | 83 | 24 | 1 | 46 | 127 | 47 | 129 | 6.74e-34 | 115 |
| MS.gene62885.t1 | MTR_4g101310 | 47.967 | 123 | 60 | 3 | 7 | 128 | 15 | 134 | 1.17e-28 | 102 |
| MS.gene62885.t1 | MTR_3g106750 | 61.905 | 84 | 31 | 1 | 46 | 128 | 46 | 129 | 2.46e-24 | 90.9 |
| MS.gene62885.t1 | MTR_5g012340 | 47.619 | 84 | 42 | 2 | 45 | 127 | 94 | 176 | 2.22e-23 | 89.7 |
| MS.gene62885.t1 | MTR_5g012380 | 47.619 | 84 | 42 | 2 | 45 | 127 | 87 | 169 | 6.08e-23 | 88.6 |
| MS.gene62885.t1 | MTR_4g101270 | 54.762 | 84 | 36 | 2 | 45 | 127 | 104 | 186 | 2.96e-21 | 84.3 |
| MS.gene62885.t1 | MTR_2g026930 | 45.570 | 79 | 41 | 1 | 49 | 127 | 122 | 198 | 5.46e-17 | 73.6 |
| MS.gene62885.t1 | MTR_2g026920 | 45.570 | 79 | 41 | 1 | 49 | 127 | 118 | 194 | 6.55e-17 | 73.2 |
| MS.gene62885.t1 | MTR_2g026775 | 46.053 | 76 | 36 | 2 | 55 | 128 | 688 | 760 | 7.19e-16 | 72.8 |
| MS.gene62885.t1 | MTR_2g026820 | 46.154 | 78 | 37 | 3 | 51 | 127 | 542 | 615 | 8.96e-13 | 63.9 |
| MS.gene62885.t1 | MTR_3g082100 | 42.857 | 84 | 46 | 2 | 45 | 127 | 253 | 335 | 1.44e-12 | 63.2 |
| MS.gene62885.t1 | MTR_4g115360 | 48.235 | 85 | 42 | 2 | 45 | 128 | 150 | 233 | 5.43e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62885.t1 | AT2G45180 | 62.205 | 127 | 46 | 1 | 2 | 128 | 10 | 134 | 1.42e-45 | 144 |
| MS.gene62885.t1 | AT5G46890 | 63.281 | 128 | 41 | 4 | 1 | 128 | 6 | 127 | 9.47e-40 | 129 |
| MS.gene62885.t1 | AT4G12520 | 51.163 | 129 | 54 | 2 | 1 | 128 | 9 | 129 | 6.20e-37 | 122 |
| MS.gene62885.t1 | AT4G12510 | 51.163 | 129 | 54 | 2 | 1 | 128 | 9 | 129 | 6.20e-37 | 122 |
| MS.gene62885.t1 | AT5G46900 | 61.719 | 128 | 43 | 3 | 1 | 128 | 6 | 127 | 1.07e-36 | 122 |
| MS.gene62885.t1 | AT1G62510 | 72.289 | 83 | 22 | 1 | 46 | 127 | 67 | 149 | 3.04e-36 | 121 |
| MS.gene62885.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 8 | 127 | 17 | 128 | 1.28e-34 | 117 |
| MS.gene62885.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 8 | 127 | 17 | 128 | 1.28e-34 | 117 |
| MS.gene62885.t1 | AT4G12480 | 40.625 | 160 | 63 | 2 | 1 | 128 | 9 | 168 | 9.92e-34 | 115 |
| MS.gene62885.t1 | AT1G12090 | 71.605 | 81 | 22 | 1 | 48 | 127 | 57 | 137 | 2.78e-33 | 113 |
| MS.gene62885.t1 | AT4G22460 | 58.095 | 105 | 40 | 3 | 22 | 125 | 29 | 130 | 3.47e-33 | 113 |
| MS.gene62885.t1 | AT4G12550 | 47.287 | 129 | 43 | 2 | 1 | 128 | 7 | 111 | 4.25e-33 | 112 |
| MS.gene62885.t1 | AT4G12490 | 62.105 | 95 | 33 | 2 | 35 | 128 | 90 | 182 | 1.95e-32 | 112 |
| MS.gene62885.t1 | AT4G12500 | 63.095 | 84 | 30 | 1 | 46 | 128 | 94 | 177 | 1.02e-31 | 110 |
| MS.gene62885.t1 | AT4G12470 | 58.427 | 89 | 36 | 1 | 41 | 128 | 73 | 161 | 1.53e-31 | 110 |
| MS.gene62885.t1 | AT4G12545 | 47.287 | 129 | 40 | 2 | 1 | 128 | 7 | 108 | 2.34e-31 | 107 |
| MS.gene62885.t1 | AT1G12100 | 45.312 | 128 | 64 | 3 | 2 | 128 | 10 | 132 | 5.52e-30 | 105 |
| MS.gene62885.t1 | AT4G12530 | 53.409 | 88 | 41 | 0 | 40 | 127 | 28 | 115 | 1.96e-27 | 98.2 |
| MS.gene62885.t1 | AT3G22142 | 52.381 | 84 | 38 | 2 | 45 | 127 | 1396 | 1478 | 4.19e-19 | 82.4 |
| MS.gene62885.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 45 | 128 | 207 | 290 | 7.44e-19 | 80.1 |
| MS.gene62885.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 45 | 128 | 207 | 290 | 7.44e-19 | 80.1 |
| MS.gene62885.t1 | AT1G62500 | 47.126 | 87 | 44 | 2 | 42 | 127 | 208 | 293 | 4.63e-18 | 78.2 |
| MS.gene62885.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 45 | 127 | 266 | 349 | 5.84e-18 | 78.2 |
| MS.gene62885.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 45 | 127 | 249 | 332 | 1.11e-17 | 77.4 |
| MS.gene62885.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 45 | 127 | 98 | 182 | 4.16e-17 | 73.9 |
| MS.gene62885.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 45 | 127 | 180 | 264 | 1.38e-16 | 73.9 |
| MS.gene62885.t1 | AT4G22490 | 35.165 | 91 | 53 | 2 | 39 | 127 | 30 | 116 | 3.45e-12 | 59.3 |
| MS.gene62885.t1 | AT4G22520 | 27.559 | 127 | 72 | 3 | 1 | 127 | 11 | 117 | 4.97e-11 | 56.2 |
Find 37 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACCAAGCTCAAGTTAA+TGG | 0.256664 | 8.3:+20000999 | None:intergenic |
| TCAAGTTAATGGGAAGATTA+AGG | 0.276650 | 8.3:+20001010 | None:intergenic |
| TGGTGTGTGTGCTGATGTAT+TGG | 0.312025 | 8.3:-20001160 | MS.gene62885:CDS |
| GTGCCTTTGCACTGCTCTTA+AGG | 0.336313 | 8.3:-20001052 | MS.gene62885:CDS |
| GGTGTGTGTGCTGATGTATT+GGG | 0.364523 | 8.3:-20001159 | MS.gene62885:CDS |
| CCATGCTGCTCTCTCATTGA+TGG | 0.378656 | 8.3:-20001099 | MS.gene62885:CDS |
| AGAGAGCAGCATGGTGTCTT+TGG | 0.413985 | 8.3:+20001108 | None:intergenic |
| CCTAGAGACACAATCAAGTT+TGG | 0.422527 | 8.3:-20001180 | MS.gene62885:CDS |
| TAAGCGCACACGAAACCCTT+TGG | 0.426755 | 8.3:+20000955 | None:intergenic |
| AGTACCAAGCTCAAGTTAAT+GGG | 0.431914 | 8.3:+20001000 | None:intergenic |
| TTGTGTCTCTAGGGCATGTT+GGG | 0.436629 | 8.3:+20001190 | None:intergenic |
| CGTGATCTTTGTGAGGTGGT+GGG | 0.442572 | 8.3:+20001250 | None:intergenic |
| CCGTGATCTTTGTGAGGTGG+TGG | 0.443706 | 8.3:+20001249 | None:intergenic |
| GTACTTAACTATTGTGGAAA+GGG | 0.444010 | 8.3:-20000982 | MS.gene62885:CDS |
| GAGCAGCATGGTGTCTTTGG+TGG | 0.477521 | 8.3:+20001111 | None:intergenic |
| ATTGTGTCTCTAGGGCATGT+TGG | 0.477867 | 8.3:+20001189 | None:intergenic |
| CCAAACTTGATTGTGTCTCT+AGG | 0.479073 | 8.3:+20001180 | None:intergenic |
| GTGATCTTTGTGAGGTGGTG+GGG | 0.487931 | 8.3:+20001251 | None:intergenic |
| GCTCTTAAGGCTAATGTCCT+AGG | 0.488278 | 8.3:-20001039 | MS.gene62885:CDS |
| TGGAGGAAGGTGGATGATGA+GGG | 0.490113 | 8.3:+20001217 | None:intergenic |
| TTGGAGGAAGGTGGATGATG+AGG | 0.490518 | 8.3:+20001216 | None:intergenic |
| TCTTCCCATTAACTTGAGCT+TGG | 0.493017 | 8.3:-20001004 | MS.gene62885:CDS |
| AGCTTGGTACTTAACTATTG+TGG | 0.494742 | 8.3:-20000988 | MS.gene62885:CDS |
| GGTTTGATTAATGTTGAGCT+TGG | 0.521860 | 8.3:-20001138 | MS.gene62885:CDS |
| TGATCTTTGTGAGGTGGTGG+GGG | 0.527911 | 8.3:+20001252 | None:intergenic |
| GGTACTTAACTATTGTGGAA+AGG | 0.533566 | 8.3:-20000983 | MS.gene62885:CDS |
| AAAATATGATGTTGAGGCAA+AGG | 0.535029 | 8.3:+20001307 | None:intergenic |
| GAAGATTAAGGTTAATGCCT+AGG | 0.539426 | 8.3:+20001022 | None:intergenic |
| CAAACTTGATTGTGTCTCTA+GGG | 0.541313 | 8.3:+20001181 | None:intergenic |
| TTGTGGAAAGGGAGTTCCAA+AGG | 0.553018 | 8.3:-20000971 | MS.gene62885:CDS |
| TGTGGAAAGGGAGTTCCAAA+GGG | 0.568343 | 8.3:-20000970 | MS.gene62885:CDS |
| CCACCACCTCACAAAGATCA+CGG | 0.588604 | 8.3:-20001249 | MS.gene62885:CDS |
| GTGGATGATGAGGGTGTGAG+TGG | 0.631843 | 8.3:+20001226 | None:intergenic |
| TAGCCTTAAGAGCAGTGCAA+AGG | 0.634750 | 8.3:+20001049 | None:intergenic |
| TGGCCGTGATCTTTGTGAGG+TGG | 0.666290 | 8.3:+20001246 | None:intergenic |
| CCATCAATGAGAGAGCAGCA+TGG | 0.704306 | 8.3:+20001099 | None:intergenic |
| GAGTGGCCGTGATCTTTGTG+AGG | 0.710036 | 8.3:+20001243 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATATGATGTTGAGGCAA+AGG | + | chr8.3:20000968-20000987 | None:intergenic | 30.0% | |
| CAGTGAAAAATATGATGTTG+AGG | + | chr8.3:20000974-20000993 | None:intergenic | 30.0% | |
| GTACTTAACTATTGTGGAAA+GGG | - | chr8.3:20001290-20001309 | MS.gene62885:CDS | 30.0% | |
| TCAAGTTAATGGGAAGATTA+AGG | + | chr8.3:20001265-20001284 | None:intergenic | 30.0% | |
| ! | CAACATCATATTTTTCACTG+TGG | - | chr8.3:20000974-20000993 | MS.gene62885:CDS | 30.0% |
| AAGTACCAAGCTCAAGTTAA+TGG | + | chr8.3:20001276-20001295 | None:intergenic | 35.0% | |
| AGCTTGGTACTTAACTATTG+TGG | - | chr8.3:20001284-20001303 | MS.gene62885:CDS | 35.0% | |
| AGTACCAAGCTCAAGTTAAT+GGG | + | chr8.3:20001275-20001294 | None:intergenic | 35.0% | |
| CAAACTTGATTGTGTCTCTA+GGG | + | chr8.3:20001094-20001113 | None:intergenic | 35.0% | |
| GAAGATTAAGGTTAATGCCT+AGG | + | chr8.3:20001253-20001272 | None:intergenic | 35.0% | |
| GGTACTTAACTATTGTGGAA+AGG | - | chr8.3:20001289-20001308 | MS.gene62885:CDS | 35.0% | |
| GGTTTGATTAATGTTGAGCT+TGG | - | chr8.3:20001134-20001153 | MS.gene62885:CDS | 35.0% | |
| CCAAACTTGATTGTGTCTCT+AGG | + | chr8.3:20001095-20001114 | None:intergenic | 40.0% | |
| CCTAGAGACACAATCAAGTT+TGG | - | chr8.3:20001092-20001111 | MS.gene62885:CDS | 40.0% | |
| ! | TCTTCCCATTAACTTGAGCT+TGG | - | chr8.3:20001268-20001287 | MS.gene62885:CDS | 40.0% |
| GCTCTTAAGGCTAATGTCCT+AGG | - | chr8.3:20001233-20001252 | MS.gene62885:CDS | 45.0% | |
| TGTGGAAAGGGAGTTCCAAA+GGG | - | chr8.3:20001302-20001321 | MS.gene62885:CDS | 45.0% | |
| TTGTGGAAAGGGAGTTCCAA+AGG | - | chr8.3:20001301-20001320 | MS.gene62885:CDS | 45.0% | |
| ! | GGTGTGTGTGCTGATGTATT+GGG | - | chr8.3:20001113-20001132 | MS.gene62885:CDS | 45.0% |
| ! | TAGCCTTAAGAGCAGTGCAA+AGG | + | chr8.3:20001226-20001245 | None:intergenic | 45.0% |
| ! | TGGTGTGTGTGCTGATGTAT+TGG | - | chr8.3:20001112-20001131 | MS.gene62885:CDS | 45.0% |
| !! | ATTGTGTCTCTAGGGCATGT+TGG | + | chr8.3:20001086-20001105 | None:intergenic | 45.0% |
| !! | CATGTTGGGTTTTTGGAGGA+AGG | + | chr8.3:20001071-20001090 | None:intergenic | 45.0% |
| !! | TCTAGGGCATGTTGGGTTTT+TGG | + | chr8.3:20001078-20001097 | None:intergenic | 45.0% |
| !! | TTGTGTCTCTAGGGCATGTT+GGG | + | chr8.3:20001085-20001104 | None:intergenic | 45.0% |
| AGAGAGCAGCATGGTGTCTT+TGG | + | chr8.3:20001167-20001186 | None:intergenic | 50.0% | |
| CCACCACCTCACAAAGATCA+CGG | - | chr8.3:20001023-20001042 | MS.gene62885:CDS | 50.0% | |
| CCATCAATGAGAGAGCAGCA+TGG | + | chr8.3:20001176-20001195 | None:intergenic | 50.0% | |
| CCATGCTGCTCTCTCATTGA+TGG | - | chr8.3:20001173-20001192 | MS.gene62885:CDS | 50.0% | |
| CGTGATCTTTGTGAGGTGGT+GGG | + | chr8.3:20001025-20001044 | None:intergenic | 50.0% | |
| GTGATCTTTGTGAGGTGGTG+GGG | + | chr8.3:20001024-20001043 | None:intergenic | 50.0% | |
| GTGCCTTTGCACTGCTCTTA+AGG | - | chr8.3:20001220-20001239 | MS.gene62885:CDS | 50.0% | |
| TAAGCGCACACGAAACCCTT+TGG | + | chr8.3:20001320-20001339 | None:intergenic | 50.0% | |
| TGGAGGAAGGTGGATGATGA+GGG | + | chr8.3:20001058-20001077 | None:intergenic | 50.0% | |
| TGGGGGACATGGAACATATG+TGG | + | chr8.3:20001006-20001025 | None:intergenic | 50.0% | |
| TTGGAGGAAGGTGGATGATG+AGG | + | chr8.3:20001059-20001078 | None:intergenic | 50.0% | |
| ! | TGATCTTTGTGAGGTGGTGG+GGG | + | chr8.3:20001023-20001042 | None:intergenic | 50.0% |
| !! | AGGGCATGTTGGGTTTTTGG+AGG | + | chr8.3:20001075-20001094 | None:intergenic | 50.0% |
| !! | GTTGGGTTTTTGGAGGAAGG+TGG | + | chr8.3:20001068-20001087 | None:intergenic | 50.0% |
| CCGTGATCTTTGTGAGGTGG+TGG | + | chr8.3:20001026-20001045 | None:intergenic | 55.0% | |
| GAGCAGCATGGTGTCTTTGG+TGG | + | chr8.3:20001164-20001183 | None:intergenic | 55.0% | |
| GAGTGGCCGTGATCTTTGTG+AGG | + | chr8.3:20001032-20001051 | None:intergenic | 55.0% | |
| TGGCCGTGATCTTTGTGAGG+TGG | + | chr8.3:20001029-20001048 | None:intergenic | 55.0% | |
| ! | GTGGATGATGAGGGTGTGAG+TGG | + | chr8.3:20001049-20001068 | None:intergenic | 55.0% |
| !! | TTGTGAGGTGGTGGGGGACA+TGG | + | chr8.3:20001017-20001036 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 20000954 | 20001340 | 20000954 | ID=MS.gene62885 |
| chr8.3 | mRNA | 20000954 | 20001340 | 20000954 | ID=MS.gene62885.t1;Parent=MS.gene62885 |
| chr8.3 | exon | 20000954 | 20001340 | 20000954 | ID=MS.gene62885.t1.exon1;Parent=MS.gene62885.t1 |
| chr8.3 | CDS | 20000954 | 20001340 | 20000954 | ID=cds.MS.gene62885.t1;Parent=MS.gene62885.t1 |
| Gene Sequence |
| Protein sequence |
