Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61588.t1 | AFK33681.1 | 95 | 119 | 6 | 0 | 1 | 119 | 103 | 221 | 7.60E-20 | 106.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61588.t1 | I3S089 | 95.0 | 119 | 6 | 0 | 1 | 119 | 103 | 221 | 5.5e-20 | 106.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene61588 | 0.838067 | 3.58E-57 | -1.69E-46 |
| MS.gene04919 | MS.gene61588 | 0.800011 | 1.76E-48 | -1.69E-46 |
| MS.gene04920 | MS.gene61588 | 0.832401 | 9.65E-56 | -1.69E-46 |
| MS.gene049283 | MS.gene61588 | 0.809459 | 1.87E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene61588 | 0.812414 | 4.29E-51 | -1.69E-46 |
| MS.gene049680 | MS.gene61588 | 0.860322 | 2.24E-63 | -1.69E-46 |
| MS.gene05029 | MS.gene61588 | 0.81718 | 3.77E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene61588 | 0.821268 | 4.42E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene61588 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene61588 | 0.830789 | 2.41E-55 | -1.69E-46 |
| MS.gene051019 | MS.gene61588 | 0.800396 | 1.47E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene61588 | 0.809891 | 1.51E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene61588 | 0.804105 | 2.53E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene61588 | 0.800672 | 1.29E-48 | -1.69E-46 |
| MS.gene051975 | MS.gene61588 | 0.809767 | 1.61E-50 | -1.69E-46 |
| MS.gene052072 | MS.gene61588 | 0.806865 | 6.68E-50 | -1.69E-46 |
| MS.gene052533 | MS.gene61588 | 0.804234 | 2.38E-49 | -1.69E-46 |
| MS.gene052793 | MS.gene61588 | 0.820451 | 6.81E-53 | -1.69E-46 |
| MS.gene053228 | MS.gene61588 | 0.800344 | 1.51E-48 | -1.69E-46 |
| MS.gene053879 | MS.gene61588 | 0.803885 | 2.82E-49 | -1.69E-46 |
| MS.gene053931 | MS.gene61588 | 0.800964 | 1.13E-48 | -1.69E-46 |
| MS.gene054004 | MS.gene61588 | 0.823002 | 1.75E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene61588 | 0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene054413 | MS.gene61588 | 0.808071 | 3.71E-50 | -1.69E-46 |
| MS.gene055150 | MS.gene61588 | 0.828747 | 7.57E-55 | -1.69E-46 |
| MS.gene055765 | MS.gene61588 | 0.812955 | 3.27E-51 | -1.69E-46 |
| MS.gene056071 | MS.gene61588 | 0.820711 | 5.94E-53 | -1.69E-46 |
| MS.gene056693 | MS.gene61588 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene05689 | MS.gene61588 | 0.804243 | 2.37E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene61588 | 0.819785 | 9.68E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene61588 | 0.832463 | 9.32E-56 | -1.69E-46 |
| MS.gene05781 | MS.gene61588 | 0.807512 | 4.88E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene61588 | 0.801945 | 7.09E-49 | -1.69E-46 |
| MS.gene058159 | MS.gene61588 | 0.820085 | 8.26E-53 | -1.69E-46 |
| MS.gene058781 | MS.gene61588 | 0.802094 | 6.60E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene61588 | 0.865473 | 5.76E-65 | -1.69E-46 |
| MS.gene05952 | MS.gene61588 | 0.808663 | 2.77E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene61588 | -0.858144 | 1.01E-62 | -1.69E-46 |
| MS.gene059901 | MS.gene61588 | -0.881551 | 2.19E-70 | -1.69E-46 |
| MS.gene060763 | MS.gene61588 | 0.835801 | 1.36E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene61588 | 0.832135 | 1.12E-55 | -1.69E-46 |
| MS.gene060937 | MS.gene61588 | 0.834909 | 2.28E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene61588 | 0.806113 | 9.63E-50 | -1.69E-46 |
| MS.gene061243 | MS.gene61588 | 0.800843 | 1.19E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61588.t1 | MTR_4g049540 | 99.160 | 119 | 1 | 0 | 1 | 119 | 103 | 221 | 1.64e-81 | 238 |
| MS.gene61588.t1 | MTR_2g088060 | 63.559 | 118 | 43 | 0 | 1 | 118 | 104 | 221 | 1.69e-46 | 149 |
| MS.gene61588.t1 | MTR_2g023880 | 55.932 | 118 | 52 | 0 | 1 | 118 | 104 | 221 | 4.03e-41 | 135 |
| MS.gene61588.t1 | MTR_7g056133 | 38.532 | 109 | 60 | 1 | 1 | 102 | 104 | 212 | 6.09e-15 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61588.t1 | AT5G42570 | 71.818 | 110 | 31 | 0 | 1 | 110 | 103 | 212 | 3.29e-49 | 156 |
| MS.gene61588.t1 | AT1G11905 | 52.336 | 107 | 51 | 0 | 1 | 107 | 103 | 209 | 1.05e-29 | 106 |
| MS.gene61588.t1 | AT1G11905 | 50.000 | 102 | 51 | 0 | 6 | 107 | 107 | 208 | 7.61e-26 | 96.7 |
Find 21 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAATCTGAGGGGTTCCTTA+TGG | 0.339577 | 4.1:-48240406 | MS.gene61588:CDS |
| TTTACCGTCCTCAAAACTTC+GGG | 0.391593 | 4.1:+48240569 | None:intergenic |
| TGGAAGACAATCAGAATCTT+CGG | 0.449833 | 4.1:-48240368 | MS.gene61588:CDS |
| TTGTTTCTTAACAGCTTCCA+TGG | 0.457783 | 4.1:+48240593 | None:intergenic |
| TGGAAGCACAGAGGAGCAAA+AGG | 0.480927 | 4.1:-48240544 | MS.gene61588:CDS |
| GAATCTGAATGCGAGGCAAA+AGG | 0.494465 | 4.1:-48240474 | MS.gene61588:CDS |
| TATGGAATATGATCGCCTCT+TGG | 0.494710 | 4.1:-48240388 | MS.gene61588:CDS |
| AGAGGCGATCATATTCCATA+AGG | 0.520476 | 4.1:+48240391 | None:intergenic |
| TATCAAGATGTGAAGAGCTC+TGG | 0.537183 | 4.1:+48240325 | None:intergenic |
| TAAGAGAGCTTCGTTTACTC+AGG | 0.542022 | 4.1:-48240620 | MS.gene61588:CDS |
| GACATTGGAAACTGAAGTAG+AGG | 0.553403 | 4.1:-48240439 | MS.gene61588:CDS |
| TCAGAATCTTCGGAGTCAGT+TGG | 0.558726 | 4.1:-48240358 | MS.gene61588:CDS |
| AGGAACCAAGGAAAAGACAT+TGG | 0.565077 | 4.1:-48240454 | MS.gene61588:CDS |
| ATGTGAAGAGCTCTGGTCGA+TGG | 0.573741 | 4.1:+48240332 | None:intergenic |
| GAATCTTCGGAGTCAGTTGG+AGG | 0.578067 | 4.1:-48240355 | MS.gene61588:CDS |
| AACGAAGCTCTCTTATGTAG+TGG | 0.615932 | 4.1:+48240628 | None:intergenic |
| TCGTTTACTCAGGAAGACCA+TGG | 0.634666 | 4.1:-48240610 | MS.gene61588:CDS |
| GAAGCTCGAATCTGAATGCG+AGG | 0.639033 | 4.1:-48240481 | MS.gene61588:CDS |
| GATTCTGATTGTCTTCCAAG+AGG | 0.650535 | 4.1:+48240373 | None:intergenic |
| ATGCGAGGCAAAAGGAACCA+AGG | 0.675638 | 4.1:-48240466 | MS.gene61588:CDS |
| CTTAATCTTAGACTTCAATG+TGG | 0.693873 | 4.1:+48240503 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTTAATCTTAGACTTCAATG+TGG | + | chr4.1:48240453-48240472 | None:intergenic | 30.0% | |
| CTCTTAAAAAGCAATCTGAG+GGG | - | chr4.1:48240537-48240556 | MS.gene61588:CDS | 35.0% | |
| GCTCTTAAAAAGCAATCTGA+GGG | - | chr4.1:48240536-48240555 | MS.gene61588:CDS | 35.0% | |
| TGGAAGACAATCAGAATCTT+CGG | - | chr4.1:48240585-48240604 | MS.gene61588:CDS | 35.0% | |
| ! | AGTTTCCAATGTCTTTTCCT+TGG | + | chr4.1:48240507-48240526 | None:intergenic | 35.0% |
| ! | TTGTTTCTTAACAGCTTCCA+TGG | + | chr4.1:48240363-48240382 | None:intergenic | 35.0% |
| ! | TTTTACCGTCCTCAAAACTT+CGG | + | chr4.1:48240388-48240407 | None:intergenic | 35.0% |
| !!! | AGTTTTGAGGACGGTAAAAA+TGG | - | chr4.1:48240389-48240408 | MS.gene61588:CDS | 35.0% |
| AACGAAGCTCTCTTATGTAG+TGG | + | chr4.1:48240328-48240347 | None:intergenic | 40.0% | |
| AGAGGCGATCATATTCCATA+AGG | + | chr4.1:48240565-48240584 | None:intergenic | 40.0% | |
| AGGAACCAAGGAAAAGACAT+TGG | - | chr4.1:48240499-48240518 | MS.gene61588:CDS | 40.0% | |
| GACATTGGAAACTGAAGTAG+AGG | - | chr4.1:48240514-48240533 | MS.gene61588:CDS | 40.0% | |
| GATTCTGATTGTCTTCCAAG+AGG | + | chr4.1:48240583-48240602 | None:intergenic | 40.0% | |
| GGCTCTTAAAAAGCAATCTG+AGG | - | chr4.1:48240535-48240554 | MS.gene61588:CDS | 40.0% | |
| TAAGAGAGCTTCGTTTACTC+AGG | - | chr4.1:48240333-48240352 | MS.gene61588:CDS | 40.0% | |
| TATCAAGATGTGAAGAGCTC+TGG | + | chr4.1:48240631-48240650 | None:intergenic | 40.0% | |
| TATGGAATATGATCGCCTCT+TGG | - | chr4.1:48240565-48240584 | MS.gene61588:CDS | 40.0% | |
| TTTACCGTCCTCAAAACTTC+GGG | + | chr4.1:48240387-48240406 | None:intergenic | 40.0% | |
| ! | GAAACAAACCCGAAGTTTTG+AGG | - | chr4.1:48240376-48240395 | MS.gene61588:CDS | 40.0% |
| GAATCTGAATGCGAGGCAAA+AGG | - | chr4.1:48240479-48240498 | MS.gene61588:CDS | 45.0% | |
| TCAGAATCTTCGGAGTCAGT+TGG | - | chr4.1:48240595-48240614 | MS.gene61588:CDS | 45.0% | |
| TCGTTTACTCAGGAAGACCA+TGG | - | chr4.1:48240343-48240362 | MS.gene61588:CDS | 45.0% | |
| ! | CGGTAAAAATGGAAGCACAG+AGG | - | chr4.1:48240400-48240419 | MS.gene61588:CDS | 45.0% |
| !!! | CAAACCCGAAGTTTTGAGGA+CGG | - | chr4.1:48240380-48240399 | MS.gene61588:CDS | 45.0% |
| ATGCGAGGCAAAAGGAACCA+AGG | - | chr4.1:48240487-48240506 | MS.gene61588:CDS | 50.0% | |
| GAAGCTCGAATCTGAATGCG+AGG | - | chr4.1:48240472-48240491 | MS.gene61588:CDS | 50.0% | |
| GAATCTTCGGAGTCAGTTGG+AGG | - | chr4.1:48240598-48240617 | MS.gene61588:CDS | 50.0% | |
| GCAATCTGAGGGGTTCCTTA+TGG | - | chr4.1:48240547-48240566 | MS.gene61588:CDS | 50.0% | |
| ! | ATGTGAAGAGCTCTGGTCGA+TGG | + | chr4.1:48240624-48240643 | None:intergenic | 50.0% |
| ! | TGGAAGCACAGAGGAGCAAA+AGG | - | chr4.1:48240409-48240428 | MS.gene61588:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 48240308 | 48240667 | 48240308 | ID=MS.gene61588 |
| chr4.1 | mRNA | 48240308 | 48240667 | 48240308 | ID=MS.gene61588.t1;Parent=MS.gene61588 |
| chr4.1 | exon | 48240308 | 48240667 | 48240308 | ID=MS.gene61588.t1.exon1;Parent=MS.gene61588.t1 |
| chr4.1 | CDS | 48240308 | 48240667 | 48240308 | ID=cds.MS.gene61588.t1;Parent=MS.gene61588.t1 |
| Gene Sequence |
| Protein sequence |
