AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31231


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31231.t1 MTR_7g062720 65.909 132 45 0 1 132 1 132 2.25e-60 190
MS.gene31231.t1 MTR_5g061150 62.992 127 47 0 20 146 10 136 6.08e-49 159
MS.gene31231.t1 MTR_4g037355 50.000 116 17 2 151 225 1 116 1.88e-26 101
MS.gene31231.t1 MTR_4g112730 75.410 61 15 0 138 198 1 61 7.35e-25 95.9
MS.gene31231.t1 MTR_1g036690 58.537 82 27 3 218 298 5 80 2.67e-23 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGGCTCTTAATATTTC+TGG 0.080746 2.4:+7151283 MS.gene31231:CDS
TTCGGCATTTAAATGAATTT+TGG 0.166319 2.4:-7151335 None:intergenic
CACTTCAAAATAAGGATAAA+AGG 0.234592 2.4:+7152178 MS.gene31231:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 2.4:+7152170 MS.gene31231:CDS
TAGAAGATCCAAAAGATCTT+TGG 0.269782 2.4:+7151473 MS.gene31231:CDS
TATTTCTGGAAATAATTATC+TGG 0.282933 2.4:+7151297 MS.gene31231:CDS
ATGGTTGTATTTGCGTTTAC+AGG 0.299146 2.4:+7151558 MS.gene31231:CDS
TATAGTTGGGGTTCTATTCC+TGG 0.328430 2.4:-7152332 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 2.4:-7152147 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 2.4:+7152230 MS.gene31231:CDS
CTGGAAATAATTATCTGGCT+TGG 0.347827 2.4:+7151302 MS.gene31231:CDS
TGGAAATAATTATCTGGCTT+GGG 0.361577 2.4:+7151303 MS.gene31231:CDS
TTTGGGAAAATTTAAAAGAA+AGG 0.385472 2.4:+7151491 MS.gene31231:CDS
AACCATTCGTTACGAGCATT+AGG 0.390171 2.4:-7151541 None:intergenic
AGAAGATCCAAAAGATCTTT+GGG 0.393236 2.4:+7151474 MS.gene31231:CDS
GAAAATGATGAAGATGATTA+TGG 0.394855 2.4:+7152222 MS.gene31231:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 2.4:+7152099 MS.gene31231:CDS
CCTTCGTCACCACCTTCATA+AGG 0.433246 2.4:+7151426 MS.gene31231:CDS
CAAAACAACTGAACAACAAA+AGG 0.454927 2.4:+7151387 MS.gene31231:CDS
AGAAATCAGCAGCATCTAAG+TGG 0.464577 2.4:-7152260 None:intergenic
AATGGTATCTCCATGCCCTT+CGG 0.470113 2.4:-7151353 None:intergenic
AATGTTGTAAAGAATGATAT+TGG 0.471241 2.4:+7152048 MS.gene31231:CDS
ATTGACACTGTGTGGAGAAA+AGG 0.483584 2.4:+7151633 MS.gene31231:CDS
GCAGCAACAATACCGCGAAA+AGG 0.486558 2.4:+7151717 MS.gene31231:CDS
ACTTCAAAATAAGGATAAAA+GGG 0.487291 2.4:+7152179 MS.gene31231:CDS
GCTACATTCACTTCTGGGAA+TGG 0.488554 2.4:-7151841 None:intergenic
TACATTAGATGCATGAAAAG+TGG 0.490606 2.4:-7151689 None:intergenic
CAGCAACAATACCGCGAAAA+GGG 0.491478 2.4:+7151718 MS.gene31231:CDS
CATTTAAATGCCGAAGGGCA+TGG 0.494088 2.4:+7151343 MS.gene31231:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 2.4:+7152106 MS.gene31231:CDS
CATGGAGATACCATTAAAGA+AGG 0.521442 2.4:+7151361 MS.gene31231:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 2.4:+7152314 MS.gene31231:CDS
AAAATTCATTTAAATGCCGA+AGG 0.537172 2.4:+7151337 MS.gene31231:CDS
TTCCTAATGCTCGTAACGAA+TGG 0.575736 2.4:+7151539 MS.gene31231:CDS
AACTGAACAACAAAAGGCTA+AGG 0.582581 2.4:+7151393 MS.gene31231:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 2.4:+7152090 MS.gene31231:CDS
ATTCGTTACGAGCATTAGGA+AGG 0.589689 2.4:-7151537 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 2.4:-7151426 None:intergenic
AGTATTTGCTATCGTTGTGG+AGG 0.599695 2.4:+7152093 MS.gene31231:CDS
AATCACACGACTCGTCCCAC+TGG 0.600994 2.4:+7151811 MS.gene31231:CDS
GAAATCAGCAGCATCTAAGT+GGG 0.621669 2.4:-7152259 None:intergenic
AAATTCATTTAAATGCCGAA+GGG 0.631745 2.4:+7151338 MS.gene31231:CDS
GTGGTGACGAAGGAATATCA+TGG 0.663314 2.4:-7151416 None:intergenic
ACTTCTCTATTGACACTGTG+TGG 0.668278 2.4:+7151625 MS.gene31231:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 2.4:-7151827 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 2.4:-7151826 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG + chr2.4:7152048-7152067 MS.gene31231:CDS 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG + chr2.4:7152179-7152198 MS.gene31231:CDS 20.0%
!! TTTGGGAAAATTTAAAAGAA+AGG + chr2.4:7151491-7151510 MS.gene31231:CDS 20.0%
!!! TATTTCTGGAAATAATTATC+TGG + chr2.4:7151297-7151316 MS.gene31231:CDS 20.0%
! AAAATTCATTTAAATGCCGA+AGG + chr2.4:7151337-7151356 MS.gene31231:CDS 25.0%
! AAATTCATTTAAATGCCGAA+GGG + chr2.4:7151338-7151357 MS.gene31231:CDS 25.0%
! CACTTCAAAATAAGGATAAA+AGG + chr2.4:7152178-7152197 MS.gene31231:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG + chr2.4:7152222-7152241 MS.gene31231:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG + chr2.4:7152230-7152249 MS.gene31231:CDS 25.0%
!! AAATTTTCCCAAAGATCTTT+TGG - chr2.4:7151484-7151503 None:intergenic 25.0%
!! ATCATTTTCACATGCAAAAT+GGG - chr2.4:7152211-7152230 None:intergenic 25.0%
!! TAAAGATCAACAAGATGTTT+TGG - chr2.4:7152150-7152169 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG - chr2.4:7152038-7152057 None:intergenic 25.0%
!! TTCGGCATTTAAATGAATTT+TGG - chr2.4:7151338-7151357 None:intergenic 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG - chr2.4:7151441-7151460 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG - chr2.4:7152014-7152033 None:intergenic 25.0%
!!! TAAATGAATTTTGGCATCAA+GGG - chr2.4:7151329-7151348 None:intergenic 25.0%
!!! TGTTTTGTTTCCTTCTTTAA+TGG - chr2.4:7151374-7151393 None:intergenic 25.0%
!!! TTAAATGAATTTTGGCATCA+AGG - chr2.4:7151330-7151349 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG + chr2.4:7151474-7151493 MS.gene31231:CDS 30.0%
CAAAACAACTGAACAACAAA+AGG + chr2.4:7151387-7151406 MS.gene31231:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG + chr2.4:7152314-7152333 MS.gene31231:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG + chr2.4:7152170-7152189 MS.gene31231:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG - chr2.4:7151692-7151711 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG + chr2.4:7151473-7151492 MS.gene31231:CDS 30.0%
! AAGATCTTTTGGATCTTCTA+TGG - chr2.4:7151473-7151492 None:intergenic 30.0%
! AGATGATCAATCTTTTCATC+AGG - chr2.4:7152294-7152313 None:intergenic 30.0%
! CATCATTTTCACATGCAAAA+TGG - chr2.4:7152212-7152231 None:intergenic 30.0%
! TGGAAATAATTATCTGGCTT+GGG + chr2.4:7151303-7151322 MS.gene31231:CDS 30.0%
! TTTGAGGCTCTTAATATTTC+TGG + chr2.4:7151283-7151302 MS.gene31231:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG - chr2.4:7151438-7151457 None:intergenic 30.0%
!! GGTTTTAATCGTAATCACAA+TGG + chr2.4:7151964-7151983 MS.gene31231:intron 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG - chr2.4:7152173-7152192 None:intergenic 30.0%
AACTGAACAACAAAAGGCTA+AGG + chr2.4:7151393-7151412 MS.gene31231:CDS 35.0%
ATGGTTGTATTTGCGTTTAC+AGG + chr2.4:7151558-7151577 MS.gene31231:CDS 35.0%
CATGGAGATACCATTAAAGA+AGG + chr2.4:7151361-7151380 MS.gene31231:CDS 35.0%
CTGGAAATAATTATCTGGCT+TGG + chr2.4:7151302-7151321 MS.gene31231:CDS 35.0%
! CCTCAAAATCAAGTTTTGCA+AGG - chr2.4:7151270-7151289 None:intergenic 35.0%
! CCTTGCAAAACTTGATTTTG+AGG + chr2.4:7151267-7151286 MS.gene31231:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG - chr2.4:7151468-7151487 None:intergenic 35.0%
!!! ACCTTTTAAAACCCTTTTCG+CGG - chr2.4:7151732-7151751 None:intergenic 35.0%
AACCATTCGTTACGAGCATT+AGG - chr2.4:7151544-7151563 None:intergenic 40.0%
ACTTCTCTATTGACACTGTG+TGG + chr2.4:7151625-7151644 MS.gene31231:CDS 40.0%
AGAAATCAGCAGCATCTAAG+TGG - chr2.4:7152263-7152282 None:intergenic 40.0%
ATTCGTTACGAGCATTAGGA+AGG - chr2.4:7151540-7151559 None:intergenic 40.0%
ATTGACACTGTGTGGAGAAA+AGG + chr2.4:7151633-7151652 MS.gene31231:CDS 40.0%
GAAATCAGCAGCATCTAAGT+GGG - chr2.4:7152262-7152281 None:intergenic 40.0%
TTCCTAATGCTCGTAACGAA+TGG + chr2.4:7151539-7151558 MS.gene31231:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG - chr2.4:7151522-7151541 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG + chr2.4:7152093-7152112 MS.gene31231:CDS 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG + chr2.4:7152090-7152109 MS.gene31231:CDS 40.0%
!!! ACCGCGAAAAGGGTTTTAAA+AGG + chr2.4:7151728-7151747 MS.gene31231:CDS 40.0%
AATGGTATCTCCATGCCCTT+CGG - chr2.4:7151356-7151375 None:intergenic 45.0%
CAGCAACAATACCGCGAAAA+GGG + chr2.4:7151718-7151737 MS.gene31231:CDS 45.0%
CATTTAAATGCCGAAGGGCA+TGG + chr2.4:7151343-7151362 MS.gene31231:CDS 45.0%
GATCACTATGGACAAACTCG+TGG + chr2.4:7151871-7151890 MS.gene31231:intron 45.0%
GCGTTGCTACATTCACTTCT+GGG - chr2.4:7151849-7151868 None:intergenic 45.0%
GTAGCAACGCATGATCACTA+TGG + chr2.4:7151859-7151878 MS.gene31231:intron 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG + chr2.4:7152106-7152125 MS.gene31231:CDS 45.0%
TGCGTTGCTACATTCACTTC+TGG - chr2.4:7151850-7151869 None:intergenic 45.0%
TGCTATCGTTGTGGAGGAAA+AGG + chr2.4:7152099-7152118 MS.gene31231:CDS 45.0%
! GCTACATTCACTTCTGGGAA+TGG - chr2.4:7151844-7151863 None:intergenic 45.0%
! GTGGTGACGAAGGAATATCA+TGG - chr2.4:7151419-7151438 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG - chr2.4:7152139-7152158 None:intergenic 45.0%
ACGTGGTCGTGGTTATGGAA+GGG + chr2.4:7151930-7151949 MS.gene31231:intron 50.0%
CCTTCGTCACCACCTTCATA+AGG + chr2.4:7151426-7151445 MS.gene31231:CDS 50.0%
GCAGCAACAATACCGCGAAA+AGG + chr2.4:7151717-7151736 MS.gene31231:CDS 50.0%
TATGGACAAACTCGTGGTCG+TGG + chr2.4:7151877-7151896 MS.gene31231:intron 50.0%
! CCTTATGAAGGTGGTGACGA+AGG - chr2.4:7151429-7151448 None:intergenic 50.0%
! TATGGAAGGGGACGTGGTTT+TGG + chr2.4:7151943-7151962 MS.gene31231:intron 50.0%
AATCACACGACTCGTCCCAC+TGG + chr2.4:7151811-7151830 MS.gene31231:CDS 55.0%
CACGTCCACGACCATAACCA+TGG - chr2.4:7151909-7151928 None:intergenic 55.0%
CATGGTTATGGTCGTGGACG+TGG + chr2.4:7151907-7151926 MS.gene31231:intron 55.0%
CGTGGTCGTGGTTATGGAAG+GGG + chr2.4:7151931-7151950 MS.gene31231:intron 55.0%
GACGTGGTCGTGGTTATGGA+AGG + chr2.4:7151929-7151948 MS.gene31231:intron 55.0%
! GGGAATGGAGCACTACCAGT+GGG - chr2.4:7151829-7151848 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG - chr2.4:7151830-7151849 None:intergenic 55.0%
CAAACTCGTGGTCGTGGTCG+TGG + chr2.4:7151883-7151902 MS.gene31231:intron 60.0%
CGTGGACGTGGTCGTGGTTA+TGG + chr2.4:7151925-7151944 MS.gene31231:intron 60.0%
CGTGGCCATGGTTATGGTCG+TGG + chr2.4:7151901-7151920 MS.gene31231:intron 60.0%
CGTGGTCGTGGCCATGGTTA+TGG + chr2.4:7151895-7151914 MS.gene31231:intron 60.0%
CGTGGTTATGGAAGGGGACG+TGG + chr2.4:7151937-7151956 MS.gene31231:intron 60.0%
TATGGTCGTGGACGTGGACG+TGG + chr2.4:7151913-7151932 MS.gene31231:intron 60.0%
CGTGGACGTGGACGTGGTCG+TGG + chr2.4:7151919-7151938 MS.gene31231:intron 70.0%
CGTGGTCGTGGTCGTGGCCA+TGG + chr2.4:7151889-7151908 MS.gene31231:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7151259 7152341 7151259 ID=MS.gene31231
chr2.4 mRNA 7151259 7152341 7151259 ID=MS.gene31231.t1;Parent=MS.gene31231
chr2.4 exon 7151259 7151852 7151259 ID=MS.gene31231.t1.exon1;Parent=MS.gene31231.t1
chr2.4 CDS 7151259 7151852 7151259 ID=cds.MS.gene31231.t1;Parent=MS.gene31231.t1
chr2.4 exon 7152039 7152341 7152039 ID=MS.gene31231.t1.exon2;Parent=MS.gene31231.t1
chr2.4 CDS 7152039 7152341 7152039 ID=cds.MS.gene31231.t1;Parent=MS.gene31231.t1
Gene Sequence

>MS.gene31231

ATGTCGAACCTTGCAAAACTTGATTTTGAGGCTCTTAATATTTCTGGAAATAATTATCTGGCTTGGGCCCTTGATGCCAAAATTCATTTAAATGCCGAAGGGCATGGAGATACCATTAAAGAAGGAAACAAAACAACTGAACAACAAAAGGCTAAGGCCATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGAAAATTTAAAAGAAAGGTTTGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAACGAATGGTTGTATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCAGCTGTATTTAAGATTACTTCTCTATTGACACTGTGTGGAGAAAAGGTGACTGATGCACAAATGTTAGAAAAAACATTCTCCACTTTTCATGCATCTAATGTAATCTTGCAGCAACAATACCGCGAAAAGGGTTTTAAAAGGTATTGTGACTTAATATCATGTCTGCTTGTAGCAGAACAAAACAACGAGCTTTTGATGAAAAATCACACGACTCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACGCATGATCACTATGGACAAACTCGTGGTCGTGGTCGTGGCCATGGTTATGGTCGTGGACGTGGACGTGGTCGTGGTTATGGAAGGGGACGTGGTTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAATACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACCCATTTTGCATGTGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTTCTTTGTTGATCCTGATGAAAAGATTGATCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MS.gene31231.t1

MSNLAKLDFEALNISGNNYLAWALDAKIHLNAEGHGDTIKEGNKTTEQQKAKAMIFLRHHLHKDLKNEYLTIEDPKDLWENLKERFDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKVTDAQMLEKTFSTFHASNVILQQQYREKGFKRYCDLISCLLVAEQNNELLMKNHTTRPTGSAPFPEKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADFFVDPDEKIDHLIRDENIQE