Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39666.t1 | XP_003626825.1 | 99 | 605 | 6 | 0 | 1 | 605 | 1 | 605 | 0.00E+00 | 1191.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39666.t1 | P37221 | 84.9 | 602 | 89 | 2 | 1 | 602 | 1 | 600 | 2.9e-297 | 1021.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39666.t1 | G7LDG5 | 99.0 | 605 | 6 | 0 | 1 | 605 | 1 | 605 | 0.0e+00 | 1191.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene39666 | 0.825219 | 5.28E-54 | -1.69E-46 |
| MS.gene049435 | MS.gene39666 | 0.802358 | 5.83E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene39666 | 0.802032 | 6.80E-49 | -1.69E-46 |
| MS.gene050228 | MS.gene39666 | 0.808517 | 2.98E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene39666 | 0.829433 | 5.16E-55 | -1.69E-46 |
| MS.gene050780 | MS.gene39666 | 0.800018 | 1.75E-48 | -1.69E-46 |
| MS.gene050820 | MS.gene39666 | 0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene051492 | MS.gene39666 | 0.805069 | 1.59E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene39666 | 0.80238 | 5.77E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene39666 | 0.838231 | 3.25E-57 | -1.69E-46 |
| MS.gene052389 | MS.gene39666 | 0.824661 | 7.15E-54 | -1.69E-46 |
| MS.gene052488 | MS.gene39666 | 0.805486 | 1.30E-49 | -1.69E-46 |
| MS.gene052493 | MS.gene39666 | 0.805957 | 1.04E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene39666 | 0.810601 | 1.06E-50 | -1.69E-46 |
| MS.gene053147 | MS.gene39666 | 0.841224 | 5.41E-58 | -1.69E-46 |
| MS.gene053166 | MS.gene39666 | 0.825763 | 3.92E-54 | -1.69E-46 |
| MS.gene053201 | MS.gene39666 | 0.813715 | 2.22E-51 | -1.69E-46 |
| MS.gene053646 | MS.gene39666 | 0.803192 | 3.92E-49 | -1.69E-46 |
| MS.gene053647 | MS.gene39666 | 0.82709 | 1.90E-54 | -1.69E-46 |
| MS.gene05414 | MS.gene39666 | 0.827673 | 1.37E-54 | -1.69E-46 |
| MS.gene05415 | MS.gene39666 | 0.83088 | 2.29E-55 | -1.69E-46 |
| MS.gene054291 | MS.gene39666 | -0.832546 | 8.89E-56 | -1.69E-46 |
| MS.gene054467 | MS.gene39666 | 0.83979 | 1.28E-57 | -1.69E-46 |
| MS.gene054468 | MS.gene39666 | 0.881332 | 2.62E-70 | -1.69E-46 |
| MS.gene054559 | MS.gene39666 | 0.806076 | 9.81E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene39666 | 0.826316 | 2.90E-54 | -1.69E-46 |
| MS.gene054581 | MS.gene39666 | 0.824432 | 8.10E-54 | -1.69E-46 |
| MS.gene05595 | MS.gene39666 | 0.831682 | 1.45E-55 | -1.69E-46 |
| MS.gene056173 | MS.gene39666 | 0.805578 | 1.25E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene39666 | 0.821987 | 3.01E-53 | -1.69E-46 |
| MS.gene057012 | MS.gene39666 | 0.82716 | 1.82E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene39666 | 0.828978 | 6.65E-55 | -1.69E-46 |
| MS.gene057755 | MS.gene39666 | 0.801201 | 1.01E-48 | -1.69E-46 |
| MS.gene058378 | MS.gene39666 | 0.829906 | 3.96E-55 | -1.69E-46 |
| MS.gene058739 | MS.gene39666 | 0.802994 | 4.31E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene39666 | 0.833909 | 4.07E-56 | -1.69E-46 |
| MS.gene060373 | MS.gene39666 | 0.814509 | 1.49E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene39666 | 0.80386 | 2.85E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene39666 | -0.80757 | 4.74E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene39666 | MS.gene055729 | PPI |
| MS.gene39666 | MS.gene52200 | PPI |
| MS.gene39666 | MS.gene27682 | PPI |
| MS.gene40254 | MS.gene39666 | PPI |
| MS.gene72021 | MS.gene39666 | PPI |
| MS.gene019525 | MS.gene39666 | PPI |
| MS.gene39666 | MS.gene05873 | PPI |
| MS.gene39666 | MS.gene055731 | PPI |
| MS.gene39666 | MS.gene36748 | PPI |
| MS.gene39666 | MS.gene055730 | PPI |
| MS.gene39666 | MS.gene27681 | PPI |
| MS.gene58341 | MS.gene39666 | PPI |
| MS.gene39666 | MS.gene011059 | PPI |
| MS.gene39666 | MS.gene023832 | PPI |
| MS.gene39666 | MS.gene072226 | PPI |
| MS.gene39666 | MS.gene36359 | PPI |
| MS.gene014608 | MS.gene39666 | PPI |
| MS.gene39666 | MS.gene27680 | PPI |
| MS.gene39666 | MS.gene015800 | PPI |
| MS.gene40704 | MS.gene39666 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39666.t1 | MTR_8g009500 | 99.008 | 605 | 6 | 0 | 1 | 605 | 1 | 605 | 0.0 | 1232 |
| MS.gene39666.t1 | MTR_8g009500 | 99.413 | 511 | 3 | 0 | 95 | 605 | 1 | 511 | 0.0 | 1037 |
| MS.gene39666.t1 | MTR_8g009500 | 99.006 | 503 | 5 | 0 | 1 | 503 | 1 | 503 | 0.0 | 1029 |
| MS.gene39666.t1 | MTR_8g032040 | 67.361 | 576 | 180 | 4 | 30 | 604 | 13 | 581 | 0.0 | 813 |
| MS.gene39666.t1 | MTR_7g076650 | 65.794 | 573 | 190 | 3 | 33 | 605 | 13 | 579 | 0.0 | 793 |
| MS.gene39666.t1 | MTR_7g076650 | 62.333 | 600 | 183 | 3 | 44 | 605 | 23 | 617 | 0.0 | 776 |
| MS.gene39666.t1 | MTR_7g076650 | 68.164 | 512 | 157 | 3 | 33 | 544 | 13 | 518 | 0.0 | 734 |
| MS.gene39666.t1 | MTR_8g014390 | 41.197 | 568 | 286 | 11 | 28 | 588 | 36 | 562 | 1.45e-141 | 424 |
| MS.gene39666.t1 | MTR_3g110330 | 43.017 | 537 | 278 | 9 | 54 | 588 | 42 | 552 | 1.53e-140 | 421 |
| MS.gene39666.t1 | MTR_8g102620 | 43.203 | 537 | 277 | 8 | 54 | 588 | 55 | 565 | 3.45e-140 | 420 |
| MS.gene39666.t1 | MTR_2g101040 | 41.725 | 568 | 283 | 11 | 28 | 588 | 89 | 615 | 2.01e-139 | 421 |
| MS.gene39666.t1 | MTR_2g101040 | 41.725 | 568 | 283 | 11 | 28 | 588 | 123 | 649 | 2.61e-139 | 421 |
| MS.gene39666.t1 | MTR_2g101040 | 41.667 | 564 | 281 | 11 | 28 | 584 | 89 | 611 | 1.07e-138 | 418 |
| MS.gene39666.t1 | MTR_2g101040 | 40.101 | 596 | 281 | 13 | 28 | 588 | 89 | 643 | 4.74e-135 | 409 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39666.t1 | AT2G13560 | 86.301 | 584 | 76 | 1 | 19 | 602 | 18 | 597 | 0.0 | 1045 |
| MS.gene39666.t1 | AT4G00570 | 66.132 | 561 | 182 | 2 | 44 | 604 | 32 | 584 | 0.0 | 780 |
| MS.gene39666.t1 | AT5G25880 | 42.831 | 537 | 279 | 9 | 54 | 588 | 52 | 562 | 4.97e-144 | 430 |
| MS.gene39666.t1 | AT5G25880 | 43.074 | 527 | 272 | 9 | 54 | 578 | 52 | 552 | 7.98e-144 | 428 |
| MS.gene39666.t1 | AT2G19900 | 43.575 | 537 | 275 | 8 | 54 | 588 | 45 | 555 | 6.47e-142 | 424 |
| MS.gene39666.t1 | AT1G79750 | 43.575 | 537 | 275 | 9 | 54 | 588 | 110 | 620 | 6.89e-142 | 426 |
| MS.gene39666.t1 | AT5G11670 | 42.644 | 537 | 280 | 8 | 54 | 588 | 52 | 562 | 5.70e-137 | 412 |
| MS.gene39666.t1 | AT2G19900 | 44.894 | 470 | 241 | 7 | 121 | 588 | 3 | 456 | 1.55e-128 | 386 |
| MS.gene39666.t1 | AT5G25880 | 42.593 | 432 | 221 | 8 | 54 | 483 | 52 | 458 | 1.44e-116 | 357 |
Find 143 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACGAAGCATTCTCCATTT+TGG | 0.169548 | 8.3:+79565399 | MS.gene39666:CDS |
| TGCTGTTATAGATGAGTTTA+TGG | 0.175785 | 8.3:+79562276 | MS.gene39666:CDS |
| TTTGCAGGAAATGGTAATAT+TGG | 0.231427 | 8.3:+79566739 | MS.gene39666:intron |
| GTTGCAATTGCTGGACTTCT+AGG | 0.233797 | 8.3:+79562910 | MS.gene39666:CDS |
| TACAGATGGGAGCAGAATTC+TGG | 0.237038 | 8.3:+79560694 | MS.gene39666:CDS |
| TGCAAGGAATTTGAAAGAAA+TGG | 0.244826 | 8.3:+79563912 | MS.gene39666:CDS |
| CAAGGAATTGGCATTACTAT+TGG | 0.271734 | 8.3:+79560737 | MS.gene39666:CDS |
| TATAATGTTGTCGCCCAAAA+TGG | 0.295757 | 8.3:-79565413 | None:intergenic |
| CGTATCCGACGGCATGTTAC+AGG | 0.306207 | 8.3:+79566937 | MS.gene39666:CDS |
| TGGATTTGACATGGCAAATA+TGG | 0.313389 | 8.3:-79565255 | None:intergenic |
| CGCCACTTGGCCAGTGCATT+AGG | 0.321819 | 8.3:-79558774 | None:intergenic |
| ACAGCTGGTGTTGCAATTGC+TGG | 0.329137 | 8.3:+79562901 | MS.gene39666:CDS |
| GATCTTGGAGTTCAAGGAAT+TGG | 0.345793 | 8.3:+79560725 | MS.gene39666:CDS |
| AGAGTGCAAAGAGTCAATTT+TGG | 0.357216 | 8.3:+79563466 | MS.gene39666:CDS |
| AAGGAATTGGCATTACTATT+GGG | 0.364280 | 8.3:+79560738 | MS.gene39666:CDS |
| CTTGCAAAAGGAAGGGCATC+TGG | 0.369192 | 8.3:-79563895 | None:intergenic |
| ATTCTGGGACTTGGAGATCT+TGG | 0.371211 | 8.3:+79560710 | MS.gene39666:CDS |
| AGCAAACCTACCCTGGAAAT+AGG | 0.377370 | 8.3:-79566789 | None:intergenic |
| CCGTTTAGTAATGTTGATCT+AGG | 0.379780 | 8.3:+79565451 | MS.gene39666:CDS |
| AATACCTGTACAATCACATT+TGG | 0.382169 | 8.3:-79562319 | None:intergenic |
| ATTTATTTCAGCATTGGTCT+TGG | 0.388679 | 8.3:+79566890 | MS.gene39666:intron |
| GAGTAGCCACATACTCTTTG+AGG | 0.390132 | 8.3:-79560797 | None:intergenic |
| TTGCAGCAGCATCGCCTTGA+TGG | 0.391714 | 8.3:+79562241 | MS.gene39666:CDS |
| AACAACATGTACCTATTTCC+AGG | 0.393408 | 8.3:+79566778 | MS.gene39666:CDS |
| CTATAACTGGCCAGCTGAGC+AGG | 0.395936 | 8.3:+79559918 | MS.gene39666:CDS |
| TGATGTCCATGGTCTATAAC+TGG | 0.411552 | 8.3:+79559905 | MS.gene39666:CDS |
| CTTGGAGATCTTGGAGTTCA+AGG | 0.414396 | 8.3:+79560719 | MS.gene39666:CDS |
| TCGACATTCTTCATGATCCT+TGG | 0.414537 | 8.3:+79556369 | MS.gene39666:CDS |
| GCAACAACTATCTTCTGCTT+GGG | 0.418709 | 8.3:-79562970 | None:intergenic |
| TTATTTATACCATTCTTTGT+TGG | 0.419412 | 8.3:-79565275 | None:intergenic |
| CTAGGTATTGGAGGCCCTCA+AGG | 0.420742 | 8.3:+79565211 | MS.gene39666:intron |
| GATCTATATGTTGCTGCTGC+TGG | 0.424864 | 8.3:+79560767 | MS.gene39666:CDS |
| TTGCTCCTTCCCTTAGTCCT+TGG | 0.432962 | 8.3:-79563939 | None:intergenic |
| TTATAGTGGTTGATCTGAAA+AGG | 0.434365 | 8.3:+79558718 | MS.gene39666:intron |
| AAAATGGAGAATGCTTCGTC+AGG | 0.436830 | 8.3:-79565397 | None:intergenic |
| AAATGGATCGCCAAGGACTA+AGG | 0.437660 | 8.3:+79563929 | MS.gene39666:CDS |
| TGTTGACGTTGAACCCTTGA+GGG | 0.438778 | 8.3:-79565225 | None:intergenic |
| GATTTAAAGACCTGCTCAGC+TGG | 0.439436 | 8.3:-79559928 | None:intergenic |
| TGCTTGGGGAAGTCAATCAT+TGG | 0.442318 | 8.3:-79562955 | None:intergenic |
| CATTTCTACCTTGATATTGA+AGG | 0.442872 | 8.3:-79567608 | None:intergenic |
| TTCCTGTGATGATTGACGTT+GGG | 0.445155 | 8.3:+79561495 | MS.gene39666:CDS |
| CTCCTAGGACTATCTGCTGT+TGG | 0.448226 | 8.3:+79564859 | MS.gene39666:CDS |
| GATAGTCCTAGGAGGACATC+AGG | 0.454256 | 8.3:-79564850 | None:intergenic |
| GGGAGCAGAATTCTGGGACT+TGG | 0.457800 | 8.3:+79560701 | MS.gene39666:CDS |
| ACAGATGGGAGCAGAATTCT+GGG | 0.459014 | 8.3:+79560695 | MS.gene39666:CDS |
| ATCTCGTTCTGTCATGGAAA+AGG | 0.463071 | 8.3:-79558312 | None:intergenic |
| AAGATAGTTGTTGCTGGTGC+TGG | 0.467131 | 8.3:+79562979 | MS.gene39666:CDS |
| ACTGTTGGAGTATATACTAT+TGG | 0.467180 | 8.3:-79559802 | None:intergenic |
| AAACGCTCAATCTGCACGTC+AGG | 0.469649 | 8.3:-79558377 | None:intergenic |
| ATAGGTACATGTTGTTTCCC+TGG | 0.472498 | 8.3:-79566771 | None:intergenic |
| TCTTTCAAATTCCTTGCAAA+AGG | 0.472671 | 8.3:-79563907 | None:intergenic |
| TGCAGCAGCATCGCCTTGAT+GGG | 0.473784 | 8.3:+79562242 | MS.gene39666:CDS |
| TACTACTGCAGTGCTGGAAT+CGG | 0.477528 | 8.3:+79563381 | MS.gene39666:intron |
| TCAAATTCCTTGCAAAAGGA+AGG | 0.481202 | 8.3:-79563903 | None:intergenic |
| CCTAGATCAACATTACTAAA+CGG | 0.484530 | 8.3:-79565451 | None:intergenic |
| CTTGTATGTCAGAACTATAG+TGG | 0.485090 | 8.3:+79559829 | MS.gene39666:CDS |
| ATGTCAAATCCAACAAAGAA+TGG | 0.485534 | 8.3:+79565266 | MS.gene39666:CDS |
| TCTCTACCCCAGGGGACAGC+TGG | 0.486859 | 8.3:+79562886 | MS.gene39666:intron |
| AGCAACAACTATCTTCTGCT+TGG | 0.489107 | 8.3:-79562971 | None:intergenic |
| AGCAGAAGGATATCATGAAA+TGG | 0.489300 | 8.3:+79567775 | MS.gene39666:CDS |
| TCGGTTTAGTATTCGCCACT+TGG | 0.489619 | 8.3:-79558787 | None:intergenic |
| CTAGGACTATCTGCTGTTGG+AGG | 0.492913 | 8.3:+79564862 | MS.gene39666:CDS |
| GTCCTACTATCGTTCATAAA+CGG | 0.494086 | 8.3:+79556342 | MS.gene39666:CDS |
| GCACATCATCATTAAACATT+CGG | 0.495254 | 8.3:-79562504 | None:intergenic |
| TGGAAGCTGTCTTTACTCGC+TGG | 0.495700 | 8.3:+79562296 | MS.gene39666:CDS |
| AATATTGGACACTGCAACCA+GGG | 0.501417 | 8.3:+79566754 | MS.gene39666:CDS |
| AGAGCTTCGCAAACTAAGTC+GGG | 0.501871 | 8.3:+79567805 | MS.gene39666:CDS |
| AAGCAGAAGATAGTTGTTGC+TGG | 0.502605 | 8.3:+79562973 | MS.gene39666:CDS |
| GAGAGCTTCGCAAACTAAGT+CGG | 0.504714 | 8.3:+79567804 | MS.gene39666:CDS |
| AATCACCGCCGGTGAGGACG+TGG | 0.518417 | 8.3:-79556277 | None:intergenic |
| CTAGGTGCTGTAAGAGCTCA+AGG | 0.520317 | 8.3:+79562928 | MS.gene39666:CDS |
| CTGGCCAAATGTGATTGTAC+AGG | 0.520962 | 8.3:+79562315 | MS.gene39666:CDS |
| CAAATTCCTTGCAAAAGGAA+GGG | 0.521173 | 8.3:-79563902 | None:intergenic |
| GTGAAGAAGAGGTTCTCAAA+GGG | 0.522056 | 8.3:+79567576 | MS.gene39666:CDS |
| ATTTCTACCTTGATATTGAA+GGG | 0.522938 | 8.3:-79567607 | None:intergenic |
| ACGTCAGGAGACACAACATT+TGG | 0.524491 | 8.3:-79558362 | None:intergenic |
| TTGTTGACGTTGAACCCTTG+AGG | 0.529789 | 8.3:-79565226 | None:intergenic |
| CTCAAACGCCACGTCCTCAC+CGG | 0.530207 | 8.3:+79556269 | MS.gene39666:CDS |
| CTCCAACAGCAGATAGTCCT+AGG | 0.532338 | 8.3:-79564861 | None:intergenic |
| TCTCATTTCTGTCATGCAGT+CGG | 0.533663 | 8.3:-79558806 | None:intergenic |
| GATATGATTGTAGTTACAGA+TGG | 0.537955 | 8.3:+79560680 | MS.gene39666:CDS |
| AAAACAATGGCAAGGATGCT+GGG | 0.541419 | 8.3:+79563423 | MS.gene39666:CDS |
| GCACTGAAATACATTCCTCT+TGG | 0.543451 | 8.3:-79559865 | None:intergenic |
| CTCCGTTTATGAACGATAGT+AGG | 0.544773 | 8.3:-79556344 | None:intergenic |
| TCAGGAGACACAACATTTGG+AGG | 0.547588 | 8.3:-79558359 | None:intergenic |
| ACCTTCTGATCCTAATGCAC+TGG | 0.547636 | 8.3:+79558764 | MS.gene39666:CDS |
| TAGGTATTGGAGGCCCTCAA+GGG | 0.547927 | 8.3:+79565212 | MS.gene39666:intron |
| CAAGATAGTCATCCCCATCA+AGG | 0.552456 | 8.3:-79562255 | None:intergenic |
| GAGACGATCTCGTTCTGTCA+TGG | 0.554372 | 8.3:-79558318 | None:intergenic |
| TAGTTGTTGCTGGTGCTGGC+AGG | 0.556281 | 8.3:+79562983 | MS.gene39666:CDS |
| AATGTTTAATGATGATGTGC+AGG | 0.562033 | 8.3:+79562507 | MS.gene39666:CDS |
| CGTTTGAGTCGTTGAAGTAA+CGG | 0.562516 | 8.3:-79556254 | None:intergenic |
| TATAGAAGAAGCTGTGGAGG+AGG | 0.562863 | 8.3:+79567748 | MS.gene39666:CDS |
| GTGAAGCCTGATGTCCTCCT+AGG | 0.564961 | 8.3:+79564844 | MS.gene39666:CDS |
| AGGAGCAAGTCTCGCAGAAG+TGG | 0.572831 | 8.3:+79563954 | MS.gene39666:CDS |
| AATGGATCGCCAAGGACTAA+GGG | 0.573558 | 8.3:+79563930 | MS.gene39666:CDS |
| ATAGTATATACTCCAACAGT+AGG | 0.579352 | 8.3:+79559805 | MS.gene39666:CDS |
| CGATCTTTCACTACGATGGA+AGG | 0.580755 | 8.3:+79556314 | MS.gene39666:CDS |
| TGCATATATGAGTGAAGAAG+AGG | 0.581793 | 8.3:+79567565 | MS.gene39666:CDS |
| GCCAGTGCATTAGGATCAGA+AGG | 0.582116 | 8.3:-79558765 | None:intergenic |
| GTCCCAACGTCAATCATCAC+AGG | 0.582608 | 8.3:-79561497 | None:intergenic |
| CTTGAAGTTCAAGCAAGAGA+TGG | 0.582823 | 8.3:+79558741 | MS.gene39666:CDS |
| TGTTATAGAAGAAGCTGTGG+AGG | 0.584699 | 8.3:+79567745 | MS.gene39666:CDS |
| AAATGGAGAATGCTTCGTCA+GGG | 0.585250 | 8.3:-79565396 | None:intergenic |
| TTGTATGTCAGAACTATAGT+GGG | 0.587455 | 8.3:+79559830 | MS.gene39666:CDS |
| AGTGAAGAAGAGGTTCTCAA+AGG | 0.587972 | 8.3:+79567575 | MS.gene39666:CDS |
| TTTGACATGGCAAATATGGC+TGG | 0.591614 | 8.3:-79565251 | None:intergenic |
| TAATATTGGACACTGCAACC+AGG | 0.593488 | 8.3:+79566753 | MS.gene39666:CDS |
| TGACATACAAGACCTACTGT+TGG | 0.598875 | 8.3:-79559817 | None:intergenic |
| GACAACATTATATTTGCTAG+TGG | 0.603217 | 8.3:+79565424 | MS.gene39666:CDS |
| GTGGAGGAGGATCTAGCAGA+AGG | 0.606861 | 8.3:+79567761 | MS.gene39666:CDS |
| TCGTGGAGAAATGATGTCCA+TGG | 0.612816 | 8.3:+79559894 | MS.gene39666:CDS |
| ATTCTTTGTTGGATTTGACA+TGG | 0.615831 | 8.3:-79565264 | None:intergenic |
| AGCTGGCCAGTTATAGACCA+TGG | 0.616327 | 8.3:-79559911 | None:intergenic |
| CTTCCTGTGATGATTGACGT+TGG | 0.616370 | 8.3:+79561494 | MS.gene39666:CDS |
| GTGCTGTAAGAGCTCAAGGA+AGG | 0.620608 | 8.3:+79562932 | MS.gene39666:CDS |
| TATTTCAGTGCTGAGGATCG+TGG | 0.621203 | 8.3:+79559877 | MS.gene39666:CDS |
| ATGCAGGGATTAATCACAGA+AGG | 0.622254 | 8.3:+79563856 | MS.gene39666:intron |
| AGCAGCCTGTAACATGCCGT+CGG | 0.623262 | 8.3:-79566942 | None:intergenic |
| TGGAGGATTGTTCTCAAGCG+AGG | 0.623454 | 8.3:+79564879 | MS.gene39666:CDS |
| GGCTCTAGGATCGTATCCGA+CGG | 0.624020 | 8.3:+79566926 | MS.gene39666:CDS |
| ATCCTAATGCACTGGCCAAG+TGG | 0.624552 | 8.3:+79558772 | MS.gene39666:CDS |
| ACAACATGTACCTATTTCCA+GGG | 0.625477 | 8.3:+79566779 | MS.gene39666:CDS |
| GTCGCGATCTTTCACTACGA+TGG | 0.627814 | 8.3:+79556310 | MS.gene39666:CDS |
| CAGAATTCGAGATATTACAA+AGG | 0.629110 | 8.3:+79567709 | MS.gene39666:intron |
| CAACAACTATCTTCTGCTTG+GGG | 0.630847 | 8.3:-79562969 | None:intergenic |
| TTACGCACTTTATTGAACCA+AGG | 0.636960 | 8.3:-79556386 | None:intergenic |
| ATATGATTGTAGTTACAGAT+GGG | 0.637411 | 8.3:+79560681 | MS.gene39666:CDS |
| AGGAATGTATTTCAGTGCTG+AGG | 0.642678 | 8.3:+79559870 | MS.gene39666:CDS |
| TGAAGTAATCACCGCCGGTG+AGG | 0.643325 | 8.3:-79556283 | None:intergenic |
| TTGAAAGAAATGGATCGCCA+AGG | 0.643697 | 8.3:+79563922 | MS.gene39666:CDS |
| ATCATCATTAAACATTCGGT+AGG | 0.645608 | 8.3:-79562500 | None:intergenic |
| GCAGCAGCATCGCCTTGATG+GGG | 0.650636 | 8.3:+79562243 | MS.gene39666:CDS |
| GATCGCCAAGGACTAAGGGA+AGG | 0.655509 | 8.3:+79563934 | MS.gene39666:CDS |
| TGTTACAGGCTGCTGCTGAG+CGG | 0.655909 | 8.3:+79566951 | MS.gene39666:CDS |
| CAGTATGAAGTAATCACCGC+CGG | 0.658289 | 8.3:-79556288 | None:intergenic |
| AAACGCCACGTCCTCACCGG+CGG | 0.658879 | 8.3:+79556272 | MS.gene39666:CDS |
| AATGGAGAATGCTTCGTCAG+GGG | 0.673016 | 8.3:-79565395 | None:intergenic |
| AGCTGTTATAGAAGAAGCTG+TGG | 0.673871 | 8.3:+79567742 | MS.gene39666:CDS |
| GGGCTGTTCAGAAGACCAAG+AGG | 0.697115 | 8.3:+79559850 | MS.gene39666:CDS |
| CAACAGCAGATAGTCCTAGG+AGG | 0.697965 | 8.3:-79564858 | None:intergenic |
| GAGACGATGATGATCTCGCG+CGG | 0.721970 | 8.3:-79556232 | None:intergenic |
| CGAGATCGTCTCGATCTACG+AGG | 0.743043 | 8.3:+79558329 | MS.gene39666:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 79556215 | 79567954 | 79556215 | ID=MS.gene39666 |
| chr8.3 | mRNA | 79556215 | 79567954 | 79556215 | ID=MS.gene39666.t1;Parent=MS.gene39666 |
| chr8.3 | exon | 79556215 | 79556400 | 79556215 | ID=MS.gene39666.t1.exon1;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79556215 | 79556400 | 79556215 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79558305 | 79558401 | 79558305 | ID=MS.gene39666.t1.exon2;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79558305 | 79558401 | 79558305 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79558724 | 79558842 | 79558724 | ID=MS.gene39666.t1.exon3;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79558724 | 79558842 | 79558724 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79559772 | 79559939 | 79559772 | ID=MS.gene39666.t1.exon4;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79559772 | 79559939 | 79559772 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79560677 | 79560805 | 79560677 | ID=MS.gene39666.t1.exon5;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79560677 | 79560805 | 79560677 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79561491 | 79561551 | 79561491 | ID=MS.gene39666.t1.exon6;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79561491 | 79561551 | 79561491 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79562233 | 79562336 | 79562233 | ID=MS.gene39666.t1.exon7;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79562233 | 79562336 | 79562233 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79562445 | 79562528 | 79562445 | ID=MS.gene39666.t1.exon8;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79562445 | 79562528 | 79562445 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79562898 | 79563004 | 79562898 | ID=MS.gene39666.t1.exon9;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79562898 | 79563004 | 79562898 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79563392 | 79563503 | 79563392 | ID=MS.gene39666.t1.exon10;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79563392 | 79563503 | 79563392 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79563862 | 79563975 | 79563862 | ID=MS.gene39666.t1.exon11;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79563862 | 79563975 | 79563862 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79564835 | 79564900 | 79564835 | ID=MS.gene39666.t1.exon12;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79564835 | 79564900 | 79564835 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79565215 | 79565287 | 79565215 | ID=MS.gene39666.t1.exon13;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79565215 | 79565287 | 79565215 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79565386 | 79565472 | 79565386 | ID=MS.gene39666.t1.exon14;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79565386 | 79565472 | 79565386 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79566746 | 79566800 | 79566746 | ID=MS.gene39666.t1.exon15;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79566746 | 79566800 | 79566746 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79566901 | 79566972 | 79566901 | ID=MS.gene39666.t1.exon16;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79566901 | 79566972 | 79566901 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79567559 | 79567621 | 79567559 | ID=MS.gene39666.t1.exon17;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79567559 | 79567621 | 79567559 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79567712 | 79567826 | 79567712 | ID=MS.gene39666.t1.exon18;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79567712 | 79567826 | 79567712 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| chr8.3 | exon | 79567949 | 79567954 | 79567949 | ID=MS.gene39666.t1.exon19;Parent=MS.gene39666.t1 |
| chr8.3 | CDS | 79567949 | 79567954 | 79567949 | ID=cds.MS.gene39666.t1;Parent=MS.gene39666.t1 |
| Gene Sequence |
| Protein sequence |
