AlfalfaGEDB | MS.gene067385

M. sativa cultivar XinJiangDaYe / MS.gene067385

KEGG: K00855
GO: GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005524 GO:0008144 GO:0016301 GO:0016740 GO:0016772 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00485.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067385.t1	TYK21083.1	98.6	73	1	0	1	73	113	185	1.30E-33	151.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067385.t1	P26302	98.6	72	1	0	1	72	75	146	8.9e-36	150.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067385.t1	A0A5D3DBY8	98.6	73	1	0	1	73	113	185	9.1e-34	151.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene067385	0.81571	8.04E-52	-1.69E-46
MS.gene049153	MS.gene067385	0.822697	2.06E-53	-1.69E-46
MS.gene049346	MS.gene067385	0.843942	1.03E-58	-1.69E-46
MS.gene049351	MS.gene067385	0.806016	1.01E-49	-1.69E-46
MS.gene049356	MS.gene067385	0.852499	4.43E-61	-1.69E-46
MS.gene049369	MS.gene067385	0.834041	3.77E-56	-1.69E-46
MS.gene049488	MS.gene067385	0.800834	1.20E-48	-1.69E-46
MS.gene049661	MS.gene067385	0.85969	3.47E-63	-1.69E-46
MS.gene049662	MS.gene067385	0.855091	7.96E-62	-1.69E-46
MS.gene049758	MS.gene067385	0.841879	3.63E-58	-1.69E-46
MS.gene049910	MS.gene067385	0.801828	7.49E-49	-1.69E-46
MS.gene050128	MS.gene067385	0.80761	4.65E-50	-1.69E-46
MS.gene050301	MS.gene067385	0.861917	7.32E-64	-1.69E-46
MS.gene050397	MS.gene067385	0.863316	2.71E-64	-1.69E-46
MS.gene050398	MS.gene067385	0.827134	1.85E-54	-1.69E-46
MS.gene050400	MS.gene067385	0.828077	1.10E-54	-1.69E-46
MS.gene050404	MS.gene067385	0.808203	3.48E-50	-1.69E-46
MS.gene050406	MS.gene067385	0.856855	2.43E-62	-1.69E-46
MS.gene050426	MS.gene067385	-0.807794	4.25E-50	-1.69E-46
MS.gene050432	MS.gene067385	0.825149	5.49E-54	-1.69E-46
MS.gene050464	MS.gene067385	0.838619	2.58E-57	-1.69E-46
MS.gene050529	MS.gene067385	0.82438	8.33E-54	-1.69E-46
MS.gene050593	MS.gene067385	0.807478	4.96E-50	-1.69E-46
MS.gene050670	MS.gene067385	0.881162	3.02E-70	-1.69E-46
MS.gene050971	MS.gene067385	0.827925	1.20E-54	-1.69E-46
MS.gene05101	MS.gene067385	0.839047	2.00E-57	-1.69E-46
MS.gene05105	MS.gene067385	0.873672	1.23E-67	-1.69E-46
MS.gene051255	MS.gene067385	0.802165	6.39E-49	-1.69E-46
MS.gene051360	MS.gene067385	0.800961	1.13E-48	-1.69E-46
MS.gene051387	MS.gene067385	0.801314	9.55E-49	-1.69E-46
MS.gene051471	MS.gene067385	0.802575	5.26E-49	-1.69E-46
MS.gene051525	MS.gene067385	0.836974	6.83E-57	-1.69E-46
MS.gene051582	MS.gene067385	0.830871	2.30E-55	-1.69E-46
MS.gene051605	MS.gene067385	0.820912	5.34E-53	-1.69E-46
MS.gene051692	MS.gene067385	0.827971	1.16E-54	-1.69E-46
MS.gene051860	MS.gene067385	0.831944	1.25E-55	-1.69E-46
MS.gene052011	MS.gene067385	-0.821208	4.56E-53	-1.69E-46
MS.gene052077	MS.gene067385	0.854943	8.79E-62	-1.69E-46
MS.gene052078	MS.gene067385	0.827961	1.17E-54	-1.69E-46
MS.gene052134	MS.gene067385	0.803111	4.07E-49	-1.69E-46
MS.gene052135	MS.gene067385	0.826312	2.91E-54	-1.69E-46
MS.gene052136	MS.gene067385	0.826108	3.25E-54	-1.69E-46
MS.gene052178	MS.gene067385	0.809911	1.50E-50	-1.69E-46
MS.gene052188	MS.gene067385	0.877521	5.88E-69	-1.69E-46
MS.gene052253	MS.gene067385	0.89385	4.28E-75	-1.69E-46
MS.gene052255	MS.gene067385	0.820727	5.89E-53	-1.69E-46
MS.gene052310	MS.gene067385	0.87806	3.81E-69	-1.69E-46
MS.gene052322	MS.gene067385	0.85474	1.01E-61	-1.69E-46
MS.gene052340	MS.gene067385	0.900278	8.60E-78	-1.69E-46
MS.gene052414	MS.gene067385	0.840766	7.13E-58	-1.69E-46
MS.gene052421	MS.gene067385	0.842775	2.10E-58	-1.69E-46
MS.gene052481	MS.gene067385	0.838036	3.65E-57	-1.69E-46
MS.gene052485	MS.gene067385	0.862465	4.97E-64	-1.69E-46
MS.gene052508	MS.gene067385	0.802312	5.96E-49	-1.69E-46
MS.gene053073	MS.gene067385	0.811352	7.31E-51	-1.69E-46
MS.gene05316	MS.gene067385	0.817522	3.16E-52	-1.69E-46
MS.gene053209	MS.gene067385	0.860392	2.13E-63	-1.69E-46
MS.gene053449	MS.gene067385	0.835091	2.05E-56	-1.69E-46
MS.gene053557	MS.gene067385	0.847012	1.51E-59	-1.69E-46
MS.gene053758	MS.gene067385	0.834469	2.94E-56	-1.69E-46
MS.gene053813	MS.gene067385	0.828931	6.83E-55	-1.69E-46
MS.gene053836	MS.gene067385	0.8578	1.28E-62	-1.69E-46
MS.gene053912	MS.gene067385	0.819439	1.16E-52	-1.69E-46
MS.gene053928	MS.gene067385	0.838626	2.57E-57	-1.69E-46
MS.gene054010	MS.gene067385	0.836977	6.82E-57	-1.69E-46
MS.gene054011	MS.gene067385	0.841716	4.01E-58	-1.69E-46
MS.gene054012	MS.gene067385	0.834982	2.19E-56	-1.69E-46
MS.gene054013	MS.gene067385	0.829339	5.44E-55	-1.69E-46
MS.gene054123	MS.gene067385	0.847402	1.18E-59	-1.69E-46
MS.gene054183	MS.gene067385	0.826756	2.28E-54	-1.69E-46
MS.gene054349	MS.gene067385	0.851401	9.09E-61	-1.69E-46
MS.gene054376	MS.gene067385	0.800578	1.35E-48	-1.69E-46
MS.gene054542	MS.gene067385	0.801852	7.41E-49	-1.69E-46
MS.gene054556	MS.gene067385	0.822256	2.61E-53	-1.69E-46
MS.gene054918	MS.gene067385	0.840322	9.32E-58	-1.69E-46
MS.gene054930	MS.gene067385	0.809594	1.75E-50	-1.69E-46
MS.gene054955	MS.gene067385	0.8053	1.43E-49	-1.69E-46
MS.gene05505	MS.gene067385	0.814415	1.56E-51	-1.69E-46
MS.gene055116	MS.gene067385	0.937841	1.80E-98	-1.69E-46
MS.gene055277	MS.gene067385	0.834897	2.30E-56	-1.69E-46
MS.gene055369	MS.gene067385	0.812823	3.49E-51	-1.69E-46
MS.gene055422	MS.gene067385	0.844479	7.37E-59	-1.69E-46
MS.gene055428	MS.gene067385	0.836982	6.80E-57	-1.69E-46
MS.gene05553	MS.gene067385	0.840314	9.37E-58	-1.69E-46
MS.gene055564	MS.gene067385	0.806106	9.66E-50	-1.69E-46
MS.gene055604	MS.gene067385	0.824044	9.99E-54	-1.69E-46
MS.gene055859	MS.gene067385	0.80126	9.79E-49	-1.69E-46
MS.gene055909	MS.gene067385	0.814979	1.17E-51	-1.69E-46
MS.gene055911	MS.gene067385	0.818515	1.88E-52	-1.69E-46
MS.gene056191	MS.gene067385	0.872543	2.93E-67	-1.69E-46
MS.gene056233	MS.gene067385	0.807203	5.67E-50	-1.69E-46
MS.gene056337	MS.gene067385	0.804121	2.51E-49	-1.69E-46
MS.gene056407	MS.gene067385	0.863054	3.27E-64	-1.69E-46
MS.gene056410	MS.gene067385	0.813636	2.31E-51	-1.69E-46
MS.gene056511	MS.gene067385	0.872166	3.92E-67	-1.69E-46
MS.gene056609	MS.gene067385	0.82454	7.64E-54	-1.69E-46
MS.gene056612	MS.gene067385	0.828887	7.00E-55	-1.69E-46
MS.gene05663	MS.gene067385	0.817619	3.00E-52	-1.69E-46
MS.gene05664	MS.gene067385	0.828891	6.99E-55	-1.69E-46
MS.gene056659	MS.gene067385	0.834239	3.36E-56	-1.69E-46
MS.gene05666	MS.gene067385	0.816052	6.74E-52	-1.69E-46
MS.gene056723	MS.gene067385	0.80471	1.90E-49	-1.69E-46
MS.gene056899	MS.gene067385	-0.821554	3.80E-53	-1.69E-46
MS.gene05702	MS.gene067385	0.892917	1.02E-74	-1.69E-46
MS.gene057124	MS.gene067385	0.847967	8.27E-60	-1.69E-46
MS.gene057331	MS.gene067385	0.801025	1.09E-48	-1.69E-46
MS.gene05733	MS.gene067385	0.815767	7.81E-52	-1.69E-46
MS.gene057396	MS.gene067385	0.855472	6.17E-62	-1.69E-46
MS.gene057448	MS.gene067385	0.853137	2.91E-61	-1.69E-46
MS.gene057449	MS.gene067385	0.85266	3.99E-61	-1.69E-46
MS.gene057450	MS.gene067385	0.852621	4.09E-61	-1.69E-46
MS.gene057476	MS.gene067385	0.800414	1.46E-48	-1.69E-46
MS.gene057734	MS.gene067385	0.807756	4.33E-50	-1.69E-46
MS.gene057749	MS.gene067385	0.884171	2.41E-71	-1.69E-46
MS.gene057782	MS.gene067385	0.805862	1.09E-49	-1.69E-46
MS.gene057796	MS.gene067385	0.813682	2.26E-51	-1.69E-46
MS.gene057823	MS.gene067385	0.868668	5.50E-66	-1.69E-46
MS.gene057824	MS.gene067385	0.802077	6.66E-49	-1.69E-46
MS.gene057825	MS.gene067385	0.8499	2.40E-60	-1.69E-46
MS.gene058002	MS.gene067385	0.875006	4.33E-68	-1.69E-46
MS.gene058007	MS.gene067385	0.842309	2.80E-58	-1.69E-46
MS.gene058019	MS.gene067385	0.802664	5.04E-49	-1.69E-46
MS.gene058082	MS.gene067385	0.804257	2.36E-49	-1.69E-46
MS.gene05811	MS.gene067385	0.850724	1.41E-60	-1.69E-46
MS.gene058142	MS.gene067385	0.828028	1.13E-54	-1.69E-46
MS.gene058293	MS.gene067385	0.800993	1.11E-48	-1.69E-46
MS.gene058365	MS.gene067385	0.815212	1.04E-51	-1.69E-46
MS.gene058376	MS.gene067385	0.804349	2.25E-49	-1.69E-46
MS.gene05842	MS.gene067385	0.802704	4.95E-49	-1.69E-46
MS.gene058444	MS.gene067385	0.801931	7.13E-49	-1.69E-46
MS.gene058660	MS.gene067385	0.850633	1.50E-60	-1.69E-46
MS.gene058700	MS.gene067385	0.821385	4.15E-53	-1.69E-46
MS.gene058710	MS.gene067385	0.817387	3.39E-52	-1.69E-46
MS.gene058787	MS.gene067385	0.805846	1.10E-49	-1.69E-46
MS.gene05886	MS.gene067385	0.845614	3.64E-59	-1.69E-46
MS.gene058928	MS.gene067385	0.8576	1.46E-62	-1.69E-46
MS.gene058948	MS.gene067385	0.804251	2.36E-49	-1.69E-46
MS.gene058949	MS.gene067385	0.855475	6.15E-62	-1.69E-46
MS.gene058995	MS.gene067385	0.848046	7.86E-60	-1.69E-46
MS.gene059011	MS.gene067385	0.848771	4.96E-60	-1.69E-46
MS.gene05907	MS.gene067385	0.886409	3.51E-72	-1.69E-46
MS.gene059082	MS.gene067385	0.827221	1.76E-54	-1.69E-46
MS.gene059141	MS.gene067385	0.823023	1.73E-53	-1.69E-46
MS.gene059285	MS.gene067385	0.856221	3.72E-62	-1.69E-46
MS.gene059294	MS.gene067385	0.844625	6.73E-59	-1.69E-46
MS.gene059366	MS.gene067385	0.817319	3.51E-52	-1.69E-46
MS.gene059420	MS.gene067385	0.857287	1.81E-62	-1.69E-46
MS.gene059462	MS.gene067385	0.844851	5.85E-59	-1.69E-46
MS.gene059518	MS.gene067385	0.818835	1.59E-52	-1.69E-46
MS.gene059597	MS.gene067385	0.84717	1.37E-59	-1.69E-46
MS.gene05959	MS.gene067385	0.807097	5.97E-50	-1.69E-46
MS.gene059674	MS.gene067385	0.826353	2.84E-54	-1.69E-46
MS.gene059676	MS.gene067385	0.833505	5.13E-56	-1.69E-46
MS.gene059688	MS.gene067385	0.895649	7.84E-76	-1.69E-46
MS.gene059692	MS.gene067385	0.892657	1.30E-74	-1.69E-46
MS.gene059695	MS.gene067385	0.894843	1.68E-75	-1.69E-46
MS.gene059711	MS.gene067385	0.80875	2.66E-50	-1.69E-46
MS.gene059807	MS.gene067385	0.865694	4.90E-65	-1.69E-46
MS.gene05984	MS.gene067385	0.847986	8.17E-60	-1.69E-46
MS.gene059876	MS.gene067385	0.865996	3.94E-65	-1.69E-46
MS.gene059894	MS.gene067385	0.831812	1.35E-55	-1.69E-46
MS.gene059974	MS.gene067385	0.813065	3.09E-51	-1.69E-46
MS.gene06037	MS.gene067385	0.866774	2.23E-65	-1.69E-46
MS.gene060403	MS.gene067385	0.891784	2.89E-74	-1.69E-46
MS.gene060407	MS.gene067385	0.811437	7.00E-51	-1.69E-46
MS.gene060469	MS.gene067385	0.800626	1.32E-48	-1.69E-46
MS.gene060495	MS.gene067385	0.828516	8.61E-55	-1.69E-46
MS.gene060804	MS.gene067385	0.828851	7.14E-55	-1.69E-46
MS.gene060835	MS.gene067385	0.80269	4.98E-49	-1.69E-46
MS.gene060837	MS.gene067385	0.838347	3.04E-57	-1.69E-46
MS.gene060986	MS.gene067385	0.811162	8.04E-51	-1.69E-46
MS.gene061041	MS.gene067385	0.817145	3.84E-52	-1.69E-46
MS.gene061049	MS.gene067385	0.899875	1.28E-77	-1.69E-46
MS.gene061051	MS.gene067385	-0.836921	7.05E-57	-1.69E-46
MS.gene061154	MS.gene067385	0.857764	1.31E-62	-1.69E-46
MS.gene061159	MS.gene067385	0.833803	4.32E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067385.t1	MTR_5g027530	100.000	72	0	0	1	72	82	153	1.94e-46	152

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067385.t1	AT1G32060	93.056	72	5	0	1	72	67	138	3.16e-43	143

Find 12 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGGATGATTACCATTCTT+TGG	0.234705	5.2:-18310666	MS.gene067385:CDS
GAATCAGGATTTCCACCTTT+TGG	0.277176	5.2:+18310725	None:intergenic
CTGATAAGTGTGTTTGAATC+AGG	0.321702	5.2:+18310710	None:intergenic
CACAACCACTGTGATATGTT+TGG	0.440482	5.2:-18310684	MS.gene067385:CDS
AGTTAAAGCTATTAAAGCTT+AGG	0.446558	5.2:-18310567	None:intergenic
TACCATTCTTTGGATAGAAC+TGG	0.449333	5.2:-18310656	MS.gene067385:CDS
TACCAGTTCTATCCAAAGAA+TGG	0.458109	5.2:+18310654	None:intergenic
AGATCAAAATCATTAGCTCT+TGG	0.471925	5.2:+18310602	None:intergenic
GGTGCAGCAGAGCCACCAAA+AGG	0.546897	5.2:-18310740	MS.gene067385:CDS
AGAACTGGTAGAAAAGAGAA+AGG	0.567928	5.2:-18310641	MS.gene067385:CDS
GCAGCAGAGCCACCAAAAGG+TGG	0.593621	5.2:-18310737	MS.gene067385:CDS
ATCATCCAAACATATCACAG+TGG	0.763501	5.2:+18310679	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTTGGATGATTACCATTCTT+TGG	-	chr5.2:18310669-18310688	MS.gene067385:CDS	30.0%
!	AGATCAAAATCATTAGCTCT+TGG	+	chr5.2:18310736-18310755	None:intergenic	30.0%
	AGAACTGGTAGAAAAGAGAA+AGG	-	chr5.2:18310694-18310713	MS.gene067385:CDS	35.0%
	ATCATCCAAACATATCACAG+TGG	+	chr5.2:18310659-18310678	None:intergenic	35.0%
	CTGATAAGTGTGTTTGAATC+AGG	+	chr5.2:18310628-18310647	None:intergenic	35.0%
	TACCAGTTCTATCCAAAGAA+TGG	+	chr5.2:18310684-18310703	None:intergenic	35.0%
	TACCATTCTTTGGATAGAAC+TGG	-	chr5.2:18310679-18310698	MS.gene067385:CDS	35.0%
!	AGAAGACTCACAAGTGTTTT+TGG	-	chr5.2:18310571-18310590	MS.gene067385:CDS	35.0%
	CACAACCACTGTGATATGTT+TGG	-	chr5.2:18310651-18310670	MS.gene067385:CDS	40.0%
!	GAATCAGGATTTCCACCTTT+TGG	+	chr5.2:18310613-18310632	None:intergenic	40.0%
!!!	AGACTCACAAGTGTTTTTGG+AGG	-	chr5.2:18310574-18310593	MS.gene067385:CDS	40.0%
!	TCAGGATTTCCACCTTTTGG+TGG	+	chr5.2:18310610-18310629	None:intergenic	45.0%
	GCAGCAGAGCCACCAAAAGG+TGG	-	chr5.2:18310598-18310617	MS.gene067385:CDS	60.0%
	GGTGCAGCAGAGCCACCAAA+AGG	-	chr5.2:18310595-18310614	MS.gene067385:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	18310568	18310789	18310568	ID=MS.gene067385
chr5.2	mRNA	18310568	18310789	18310568	ID=MS.gene067385.t1;Parent=MS.gene067385
chr5.2	exon	18310568	18310789	18310568	ID=MS.gene067385.t1.exon1;Parent=MS.gene067385.t1
chr5.2	CDS	18310568	18310789	18310568	ID=cds.MS.gene067385.t1;Parent=MS.gene067385.t1

Gene Sequence	>MS.gene067385 ATGAGAAGACTCACAAGTGTTTTTGGAGGTGCAGCAGAGCCACCAAAAGGTGGAAATCCTGATTCAAACACACTTATCAGTGACACAACCACTGTGATATGTTTGGATGATTACCATTCTTTGGATAGAACTGGTAGAAAAGAGAAAGGTGTTACTGCACTTGATCCAAGAGCTAATGATTTTGATCTTATGTATGAACAAGTTAAAGCTATTAAAGCTTAG
Protein sequence	>MS.gene067385.t1 MRRLTSVFGGAAEPPKGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPRANDFDLMYEQVKAIKA