AlfalfaGEDB | MS.gene65683

M. sativa cultivar XinJiangDaYe / MS.gene65683

KEGG: K15397
GO: GO:0003674 GO:0003824 GO:0005575 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016020 GO:0016053 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0071704 GO:0072330 GO:0110165 GO:1901576
PFAM: PF08392.15 PF08541.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65683.t1	XP_003614634.1	99.5	415	2	0	1	415	82	496	3.40E-240	840.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65683.t1	Q9XF43	85.1	415	62	0	1	415	83	497	3.9e-213	741.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65683.t1	G7K1J8	99.5	415	2	0	1	415	82	496	2.4e-240	840.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene65683	0.825311	5.02E-54	-1.69E-46
MS.gene049491	MS.gene65683	0.816739	4.73E-52	-1.69E-46
MS.gene049661	MS.gene65683	0.809964	1.46E-50	-1.69E-46
MS.gene049681	MS.gene65683	0.851391	9.15E-61	-1.69E-46
MS.gene050128	MS.gene65683	0.807627	4.61E-50	-1.69E-46
MS.gene050301	MS.gene65683	0.822996	1.76E-53	-1.69E-46
MS.gene050397	MS.gene65683	0.820145	8.01E-53	-1.69E-46
MS.gene050670	MS.gene65683	0.830724	2.50E-55	-1.69E-46
MS.gene05101	MS.gene65683	0.813837	2.09E-51	-1.69E-46
MS.gene05105	MS.gene65683	0.832829	7.56E-56	-1.69E-46
MS.gene051255	MS.gene65683	0.812459	4.19E-51	-1.69E-46
MS.gene051360	MS.gene65683	0.800179	1.63E-48	-1.69E-46
MS.gene051387	MS.gene65683	0.820765	5.77E-53	-1.69E-46
MS.gene051525	MS.gene65683	0.800926	1.15E-48	-1.69E-46
MS.gene052011	MS.gene65683	-0.803222	3.86E-49	-1.69E-46
MS.gene052188	MS.gene65683	0.832646	8.39E-56	-1.69E-46
MS.gene052253	MS.gene65683	0.823435	1.39E-53	-1.69E-46
MS.gene052310	MS.gene65683	0.81307	3.08E-51	-1.69E-46
MS.gene052340	MS.gene65683	0.827178	1.81E-54	-1.69E-46
MS.gene052421	MS.gene65683	0.807897	4.04E-50	-1.69E-46
MS.gene052481	MS.gene65683	0.803952	2.73E-49	-1.69E-46
MS.gene052485	MS.gene65683	0.854689	1.04E-61	-1.69E-46
MS.gene05316	MS.gene65683	0.803811	2.92E-49	-1.69E-46
MS.gene053758	MS.gene65683	0.819259	1.28E-52	-1.69E-46
MS.gene053813	MS.gene65683	0.90045	7.24E-78	-1.69E-46
MS.gene053836	MS.gene65683	0.813784	2.15E-51	-1.69E-46
MS.gene053873	MS.gene65683	0.802224	6.21E-49	-1.69E-46
MS.gene054010	MS.gene65683	0.855567	5.79E-62	-1.69E-46
MS.gene054011	MS.gene65683	0.844461	7.45E-59	-1.69E-46
MS.gene054012	MS.gene65683	0.850822	1.32E-60	-1.69E-46
MS.gene054013	MS.gene65683	0.854381	1.28E-61	-1.69E-46
MS.gene054183	MS.gene65683	0.814344	1.62E-51	-1.69E-46
MS.gene054349	MS.gene65683	0.818705	1.70E-52	-1.69E-46
MS.gene054556	MS.gene65683	0.800635	1.31E-48	-1.69E-46
MS.gene055116	MS.gene65683	0.860311	2.26E-63	-1.69E-46
MS.gene055277	MS.gene65683	0.829622	4.64E-55	-1.69E-46
MS.gene055428	MS.gene65683	0.802166	6.38E-49	-1.69E-46
MS.gene055604	MS.gene65683	0.837163	6.11E-57	-1.69E-46
MS.gene055909	MS.gene65683	0.823571	1.29E-53	-1.69E-46
MS.gene055911	MS.gene65683	0.837365	5.43E-57	-1.69E-46
MS.gene056191	MS.gene65683	0.893371	6.69E-75	-1.69E-46
MS.gene056407	MS.gene65683	0.837209	5.95E-57	-1.69E-46
MS.gene056410	MS.gene65683	0.815742	7.91E-52	-1.69E-46
MS.gene056422	MS.gene65683	0.811199	7.89E-51	-1.69E-46
MS.gene056511	MS.gene65683	0.818854	1.58E-52	-1.69E-46
MS.gene056659	MS.gene65683	0.801663	8.10E-49	-1.69E-46
MS.gene056723	MS.gene65683	0.808334	3.26E-50	-1.69E-46
MS.gene056902	MS.gene65683	0.835687	1.45E-56	-1.69E-46
MS.gene056931	MS.gene65683	0.835404	1.71E-56	-1.69E-46
MS.gene056933	MS.gene65683	0.83383	4.26E-56	-1.69E-46
MS.gene056935	MS.gene65683	0.827185	1.80E-54	-1.69E-46
MS.gene056942	MS.gene65683	0.829698	4.45E-55	-1.69E-46
MS.gene05702	MS.gene65683	0.83557	1.55E-56	-1.69E-46
MS.gene057146	MS.gene65683	0.814169	1.77E-51	-1.69E-46
MS.gene057448	MS.gene65683	0.80617	9.37E-50	-1.69E-46
MS.gene057450	MS.gene65683	0.805118	1.56E-49	-1.69E-46
MS.gene057469	MS.gene65683	0.805789	1.13E-49	-1.69E-46
MS.gene057476	MS.gene65683	0.80954	1.80E-50	-1.69E-46
MS.gene057749	MS.gene65683	0.877724	5.00E-69	-1.69E-46
MS.gene057779	MS.gene65683	0.820726	5.89E-53	-1.69E-46
MS.gene057823	MS.gene65683	0.877985	4.05E-69	-1.69E-46
MS.gene057824	MS.gene65683	0.839634	1.41E-57	-1.69E-46
MS.gene057825	MS.gene65683	0.814764	1.30E-51	-1.69E-46
MS.gene057826	MS.gene65683	0.831713	1.43E-55	-1.69E-46
MS.gene058002	MS.gene65683	0.830426	2.96E-55	-1.69E-46
MS.gene058007	MS.gene65683	0.822328	2.51E-53	-1.69E-46
MS.gene058019	MS.gene65683	0.810985	8.78E-51	-1.69E-46
MS.gene05811	MS.gene65683	0.813604	2.35E-51	-1.69E-46
MS.gene058660	MS.gene65683	0.847398	1.19E-59	-1.69E-46
MS.gene058928	MS.gene65683	0.835717	1.43E-56	-1.69E-46
MS.gene058949	MS.gene65683	0.817446	3.28E-52	-1.69E-46
MS.gene058995	MS.gene65683	0.894672	1.98E-75	-1.69E-46
MS.gene05907	MS.gene65683	0.861741	8.29E-64	-1.69E-46
MS.gene059082	MS.gene65683	0.812099	5.03E-51	-1.69E-46
MS.gene059141	MS.gene65683	0.802693	4.97E-49	-1.69E-46
MS.gene059294	MS.gene65683	0.857109	2.04E-62	-1.69E-46
MS.gene059366	MS.gene65683	0.818003	2.46E-52	-1.69E-46
MS.gene059368	MS.gene65683	0.800505	1.40E-48	-1.69E-46
MS.gene059420	MS.gene65683	0.840383	8.99E-58	-1.69E-46
MS.gene059462	MS.gene65683	0.828528	8.55E-55	-1.69E-46
MS.gene059597	MS.gene65683	0.828735	7.62E-55	-1.69E-46
MS.gene059674	MS.gene65683	0.819169	1.34E-52	-1.69E-46
MS.gene059676	MS.gene65683	0.816196	6.26E-52	-1.69E-46
MS.gene059688	MS.gene65683	0.844359	7.94E-59	-1.69E-46
MS.gene059692	MS.gene65683	0.841309	5.14E-58	-1.69E-46
MS.gene059695	MS.gene65683	0.84653	2.05E-59	-1.69E-46
MS.gene059711	MS.gene65683	0.833598	4.86E-56	-1.69E-46
MS.gene059807	MS.gene65683	0.828755	7.54E-55	-1.69E-46
MS.gene059876	MS.gene65683	0.831042	2.09E-55	-1.69E-46
MS.gene059974	MS.gene65683	0.835701	1.44E-56	-1.69E-46
MS.gene060005	MS.gene65683	0.83529	1.83E-56	-1.69E-46
MS.gene06037	MS.gene65683	0.823204	1.57E-53	-1.69E-46
MS.gene060403	MS.gene65683	0.820296	7.39E-53	-1.69E-46
MS.gene060559	MS.gene65683	0.82954	4.86E-55	-1.69E-46
MS.gene060804	MS.gene65683	0.80126	9.79E-49	-1.69E-46
MS.gene060837	MS.gene65683	0.81946	1.15E-52	-1.69E-46
MS.gene060986	MS.gene65683	0.804838	1.78E-49	-1.69E-46
MS.gene061049	MS.gene65683	0.838136	3.44E-57	-1.69E-46
MS.gene061051	MS.gene65683	-0.805674	1.19E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65683.t1	MTR_5g056510	99.518	415	2	0	1	415	82	496	0.0	861
MS.gene65683.t1	MTR_1g110930	92.771	415	30	0	1	415	82	496	0.0	814
MS.gene65683.t1	MTR_4g070270	68.704	409	128	0	1	409	94	502	0.0	611
MS.gene65683.t1	MTR_1g041085	65.385	416	143	1	1	415	103	518	0.0	572
MS.gene65683.t1	MTR_1g076430	63.942	416	149	1	1	415	97	512	0.0	570
MS.gene65683.t1	MTR_2g054940	65.133	413	143	1	1	412	96	508	0.0	567
MS.gene65683.t1	MTR_7g118170	64.146	410	139	3	7	410	122	529	0.0	555
MS.gene65683.t1	MTR_4g113280	61.836	414	144	3	1	412	85	486	0.0	539
MS.gene65683.t1	MTR_7g081695	63.391	407	136	2	1	406	96	490	0.0	531
MS.gene65683.t1	MTR_3g105550	56.905	420	170	3	1	409	117	536	2.76e-180	513
MS.gene65683.t1	MTR_2g007020	54.015	411	185	3	2	410	44	452	2.60e-169	482
MS.gene65683.t1	MTR_4g054415	53.431	408	186	3	5	409	19	425	5.16e-158	452
MS.gene65683.t1	MTR_4g054630	53.431	408	186	3	5	409	60	466	7.31e-158	454
MS.gene65683.t1	MTR_1g102290	52.334	407	192	2	3	407	78	484	1.41e-157	453
MS.gene65683.t1	MTR_2g436480	52.099	405	191	2	7	409	48	451	1.74e-156	449
MS.gene65683.t1	MTR_6g074880	53.902	410	185	3	5	411	62	470	1.15e-155	448
MS.gene65683.t1	MTR_2g013740	53.671	395	180	1	4	398	109	500	3.54e-155	448
MS.gene65683.t1	MTR_7g118160	52.567	409	149	4	6	411	55	421	7.38e-154	442
MS.gene65683.t1	MTR_3g072940	51.843	407	193	3	3	407	78	483	1.48e-150	436
MS.gene65683.t1	MTR_3g072980	51.843	407	193	3	3	407	78	483	1.63e-150	436
MS.gene65683.t1	MTR_6g032965	52.815	373	175	1	39	410	13	385	1.54e-147	424
MS.gene65683.t1	MTR_6g051440	51.117	403	194	3	7	407	81	482	1.43e-139	407
MS.gene65683.t1	MTR_1g115560	39.394	429	230	6	3	406	28	451	7.23e-110	332
MS.gene65683.t1	MTR_4g062270	40.244	410	236	5	3	406	27	433	1.80e-108	327
MS.gene65683.t1	MTR_2g096210	41.135	423	229	7	3	408	27	446	6.49e-108	327
MS.gene65683.t1	MTR_4g062190	39.623	424	228	7	3	406	27	442	5.67e-107	324
MS.gene65683.t1	MTR_4g062170	40.191	418	233	6	3	406	27	441	3.72e-104	317
MS.gene65683.t1	MTR_4g054260	49.805	257	108	4	156	409	87	325	9.08e-80	249
MS.gene65683.t1	MTR_4g053905	56.875	160	66	2	252	409	1	159	1.23e-59	191
MS.gene65683.t1	MTR_7g086260	69.355	62	17	1	247	306	22	83	2.99e-19	82.4
MS.gene65683.t1	MTR_4g054265	37.000	100	63	0	5	104	19	118	9.50e-17	76.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene65683.t1	AT1G68530	85.060	415	62	0	1	415	83	497	0.0	761
MS.gene65683.t1	AT1G25450	82.892	415	71	0	1	415	78	492	0.0	752
MS.gene65683.t1	AT1G19440	68.059	407	130	0	1	407	106	512	0.0	615
MS.gene65683.t1	AT2G16280	68.460	409	129	0	1	409	102	510	0.0	613
MS.gene65683.t1	AT4G34510	65.122	410	142	1	1	409	76	485	0.0	578
MS.gene65683.t1	AT2G26640	62.228	413	155	1	1	412	94	506	0.0	551
MS.gene65683.t1	AT1G04220	62.500	416	148	3	3	410	101	516	0.0	548
MS.gene65683.t1	AT5G43760	63.007	419	146	4	3	413	107	524	0.0	543
MS.gene65683.t1	AT1G01120	60.827	411	159	1	3	411	118	528	0.0	542
MS.gene65683.t1	AT2G15090	57.554	417	169	2	1	409	63	479	0.0	520
MS.gene65683.t1	AT4G34520	56.699	418	170	2	3	409	73	490	4.31e-179	509
MS.gene65683.t1	AT2G26250	54.713	435	168	3	1	407	115	548	1.45e-176	504
MS.gene65683.t1	AT4G34250	55.904	415	177	1	1	409	73	487	1.75e-175	499
MS.gene65683.t1	AT2G46720	53.902	410	186	1	3	409	56	465	1.93e-174	496
MS.gene65683.t1	AT1G68530	78.521	284	55	1	1	284	83	360	2.73e-167	474
MS.gene65683.t1	AT3G10280	52.439	410	185	2	3	409	56	458	2.74e-166	474
MS.gene65683.t1	AT1G71160	51.225	408	197	2	1	407	40	446	1.01e-160	461
MS.gene65683.t1	AT5G49070	52.311	411	186	2	7	411	51	457	4.13e-153	441
MS.gene65683.t1	AT3G52160	45.729	398	184	3	2	398	83	449	4.18e-130	382
MS.gene65683.t1	AT3G52160	47.922	361	156	3	39	398	7	336	1.50e-123	361
MS.gene65683.t1	AT2G28630	42.080	423	223	8	3	406	27	446	2.22e-111	335
MS.gene65683.t1	AT2G28630	42.080	423	223	8	3	406	27	446	2.22e-111	335
MS.gene65683.t1	AT5G04530	40.777	412	229	6	5	406	31	437	2.63e-108	327
MS.gene65683.t1	AT1G07720	40.811	419	225	6	8	406	32	447	1.28e-107	326
MS.gene65683.t1	AT1G07720	40.811	419	225	6	8	406	75	490	3.22e-107	326
MS.gene65683.t1	AT5G49070	50.190	263	131	0	7	269	51	313	2.06e-88	271

Find 80 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACACGTCTTCTTCATCTTTA+TGG	0.130721	5.2:-48496171	MS.gene65683:CDS
CGTCCCTTTCGCTACTTTCA+TGG	0.215185	5.2:-48497133	MS.gene65683:CDS
TTCACTTCAAAAGGATCTTA+TGG	0.218445	5.2:-48496464	MS.gene65683:CDS
AGGACCAATAGTTGTTATAT+TGG	0.251699	5.2:+48496417	None:intergenic
CGTCTTGCTTCTTCCATTGT+TGG	0.258187	5.2:+48496985	None:intergenic
GCTTCTTCCATTGTTGGTTT+AGG	0.276327	5.2:+48496991	None:intergenic
TGAAGAGAGGTGATAGAGTA+TGG	0.287194	5.2:-48496114	MS.gene65683:CDS
AGCATTAAGACACCTGTTAA+TGG	0.300750	5.2:-48496034	MS.gene65683:CDS
TGTTTGAAATCAGGAATATA+AGG	0.323168	5.2:+48496316	None:intergenic
TTTAGCCCGCGATCTTCTTC+AGG	0.324454	5.2:-48496746	MS.gene65683:CDS
GGTGGGATGTAATGGATTGC+TGG	0.333428	5.2:+48497012	None:intergenic
GAAGACAGAGAAGGTAAAGT+TGG	0.348142	5.2:-48496490	MS.gene65683:CDS
GAATGACGCTGCATCGATTC+GGG	0.353166	5.2:-48496195	MS.gene65683:CDS
ACAACAACTGCGTTTGAATT+CGG	0.360073	5.2:+48496718	None:intergenic
AGAGTATGGCAAATAGCTTT+TGG	0.363430	5.2:-48496100	MS.gene65683:CDS
AGCTTCAACCTCTCCGGAAT+GGG	0.368697	5.2:-48496796	MS.gene65683:CDS
ACCAAACTGTTTATTCAGAA+TGG	0.377309	5.2:-48496638	MS.gene65683:CDS
ATTCTCGAACGCTCTGGTCT+TGG	0.385049	5.2:-48497054	MS.gene65683:CDS
AAATCCAATATAACAACTAT+TGG	0.388103	5.2:-48496421	MS.gene65683:CDS
AGCGTTCGAGAATTCTCATT+TGG	0.392840	5.2:+48497064	None:intergenic
AACTCCTTCTTTGTCTGCTA+TGG	0.395630	5.2:-48496854	MS.gene65683:CDS
AGTTGGAATTTCACTTCAAA+AGG	0.400364	5.2:-48496473	MS.gene65683:CDS
TCTTCCCAAGGTCCATTAAC+AGG	0.401821	5.2:+48496022	None:intergenic
TCAAACGCTTGTTTGAAATC+AGG	0.404416	5.2:+48496307	None:intergenic
TCTTCCATTGTTGGTTTAGG+TGG	0.408011	5.2:+48496994	None:intergenic
CCCATTCTGAATAAACAGTT+TGG	0.412390	5.2:+48496637	None:intergenic
ATAAGAATGTCTATGTCCTT+AGG	0.415605	5.2:+48496901	None:intergenic
AGAATGACGCTGCATCGATT+CGG	0.416495	5.2:-48496196	MS.gene65683:CDS
GATACCTTGTTTGCGAAAAC+CGG	0.424265	5.2:-48496931	MS.gene65683:CDS
TTGGTTTAGGTGGGATGTAA+TGG	0.427468	5.2:+48497004	None:intergenic
CCAAACTGTTTATTCAGAAT+GGG	0.431991	5.2:-48496637	MS.gene65683:CDS
ATTTAGAGCTTAACAATCTC+AGG	0.432547	5.2:+48495971	None:intergenic
CTTCCATTGTTGGTTTAGGT+GGG	0.436694	5.2:+48496995	None:intergenic
AAAGGGACGCGGCAAGTAAT+CGG	0.440585	5.2:+48497147	None:intergenic
TTGAACTATATTGAATCAAA+AGG	0.463579	5.2:-48496142	MS.gene65683:CDS
GACACCTGTTAATGGACCTT+GGG	0.463994	5.2:-48496026	MS.gene65683:CDS
ACAATCTCAGGAATATGAAC+AGG	0.469213	5.2:+48495983	None:intergenic
ACCCGCGCTACATCCCATTC+CGG	0.483459	5.2:+48496783	None:intergenic
GCTTGTTATCTTCTCATCCA+TGG	0.488002	5.2:-48496953	MS.gene65683:CDS
AAGAGTAGTTGTTCTGAAGC+AGG	0.493059	5.2:+48496385	None:intergenic
ATCAAGAGCTTCAACCTCTC+CGG	0.495473	5.2:-48496802	MS.gene65683:CDS
GAGCTTCAACCTCTCCGGAA+TGG	0.499688	5.2:-48496797	MS.gene65683:CDS
TATGTCCTTAGGTTTGAGTC+CGG	0.505098	5.2:+48496912	None:intergenic
AGACACCTGTTAATGGACCT+TGG	0.510300	5.2:-48496027	MS.gene65683:CDS
ATGAGAATTCTCGAACGCTC+TGG	0.512522	5.2:-48497060	MS.gene65683:CDS
TCCGGAATGGGATGTAGCGC+GGG	0.514072	5.2:-48496784	MS.gene65683:CDS
AGTAATCGGTGGTTTGAAGC+AGG	0.518013	5.2:+48497161	None:intergenic
AAGGATCTTATGGCTATAGC+AGG	0.535171	5.2:-48496454	MS.gene65683:CDS
CTCCGGAATGGGATGTAGCG+CGG	0.535668	5.2:-48496785	MS.gene65683:CDS
TTTCGCAAACAAGGTATCCA+TGG	0.546419	5.2:+48496936	None:intergenic
AAGAAGGAGTTGGAGAAAAG+AGG	0.546500	5.2:+48496866	None:intergenic
ATAGCAGACAAAGAAGGAGT+TGG	0.559010	5.2:+48496856	None:intergenic
CATCCCACCTAAACCAACAA+TGG	0.566152	5.2:-48496998	MS.gene65683:CDS
AAGATCGCGGGCTAAATCGA+TGG	0.572509	5.2:+48496753	None:intergenic
TCAAAAGGAAGGATGAAGAG+AGG	0.574451	5.2:-48496127	MS.gene65683:CDS
ATTACTCCAAACTATTACCA+AGG	0.582645	5.2:-48496682	MS.gene65683:CDS
GTTCCATGAAAGTAGCGAAA+GGG	0.584347	5.2:+48497130	None:intergenic
TGAAAGTAGCGAAAGGGACG+CGG	0.588590	5.2:+48497136	None:intergenic
TTCGAAGAAGAAGACAGAGA+AGG	0.591426	5.2:-48496499	MS.gene65683:CDS
ACTATATTGAATCAAAAGGA+AGG	0.597855	5.2:-48496138	MS.gene65683:CDS
CTTCTGTAACTCATCGATCA+CGG	0.605553	5.2:+48496255	None:intergenic
ATCACCATAGCAGACAAAGA+AGG	0.610156	5.2:+48496850	None:intergenic
ATAAACCTGAAGAAGATCGC+GGG	0.612944	5.2:+48496741	None:intergenic
AACTGTTTATTCAGAATGGG+TGG	0.613269	5.2:-48496634	MS.gene65683:CDS
GGATGAGAAGATAACAAGCT+CGG	0.613593	5.2:+48496957	None:intergenic
TGTTCCATGAAAGTAGCGAA+AGG	0.619184	5.2:+48497129	None:intergenic
CTTCTTCTTCGAAAACGCAA+CGG	0.633833	5.2:+48496509	None:intergenic
GAAAACCGGACTCAAACCTA+AGG	0.634039	5.2:-48496917	MS.gene65683:CDS
TGAAGCAGGCATAATCGACT+AGG	0.636758	5.2:+48497175	None:intergenic
TCTGAAGCAGGTAGAACAAG+AGG	0.638656	5.2:+48496397	None:intergenic
CGATCAATGCAATCTTCCCA+AGG	0.642968	5.2:+48496010	None:intergenic
GCTATCTGCTGAACATGTCG+AGG	0.645995	5.2:-48496224	MS.gene65683:CDS
CGCTACATCCCATTCCGGAG+AGG	0.648049	5.2:+48496788	None:intergenic
CAAGTTGTTAGAACTCACAA+AGG	0.665486	5.2:-48496550	MS.gene65683:CDS
GATAAACCTGAAGAAGATCG+CGG	0.670461	5.2:+48496740	None:intergenic
TCGACTAGGTAGATTGCGCG+TGG	0.676158	5.2:+48497189	None:intergenic
GTTGTTAGAACTCACAAAGG+AGG	0.692073	5.2:-48496547	MS.gene65683:CDS
GGGACGCGGCAAGTAATCGG+TGG	0.693695	5.2:+48497150	None:intergenic
GGGATGTAATGGATTGCTGG+TGG	0.704587	5.2:+48497015	None:intergenic
TTAAATGCAATAGTGCTGTG+TGG	0.711523	5.2:-48496069	MS.gene65683:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATCCAATATAACAACTAT+TGG	-	chr5.2:48496750-48496769	MS.gene65683:CDS	20.0%
!!	TTGAACTATATTGAATCAAA+AGG	-	chr5.2:48497029-48497048	MS.gene65683:CDS	20.0%
!	ACTATATTGAATCAAAAGGA+AGG	-	chr5.2:48497033-48497052	MS.gene65683:CDS	25.0%
!!	TGTTTGAAATCAGGAATATA+AGG	+	chr5.2:48496858-48496877	None:intergenic	25.0%
	ACCAAACTGTTTATTCAGAA+TGG	-	chr5.2:48496533-48496552	MS.gene65683:CDS	30.0%
	AGGACCAATAGTTGTTATAT+TGG	+	chr5.2:48496757-48496776	None:intergenic	30.0%
	AGTTGGAATTTCACTTCAAA+AGG	-	chr5.2:48496698-48496717	MS.gene65683:CDS	30.0%
	ATAAGAATGTCTATGTCCTT+AGG	+	chr5.2:48496273-48496292	None:intergenic	30.0%
	ATTACTCCAAACTATTACCA+AGG	-	chr5.2:48496489-48496508	MS.gene65683:CDS	30.0%
	CCAAACTGTTTATTCAGAAT+GGG	-	chr5.2:48496534-48496553	MS.gene65683:CDS	30.0%
	TTCACTTCAAAAGGATCTTA+TGG	-	chr5.2:48496707-48496726	MS.gene65683:CDS	30.0%
!	GGAATATAAGGCTTCATTTT+AGG	+	chr5.2:48496846-48496865	None:intergenic	30.0%
!!	TCTTTTCCTTGGTAATAGTT+TGG	+	chr5.2:48496498-48496517	None:intergenic	30.0%
	AACTGTTTATTCAGAATGGG+TGG	-	chr5.2:48496537-48496556	MS.gene65683:CDS	35.0%
	ACAACAACTGCGTTTGAATT+CGG	+	chr5.2:48496456-48496475	None:intergenic	35.0%
	ACAATCTCAGGAATATGAAC+AGG	+	chr5.2:48497191-48497210	None:intergenic	35.0%
	ACACGTCTTCTTCATCTTTA+TGG	-	chr5.2:48497000-48497019	MS.gene65683:CDS	35.0%
	AGCATTAAGACACCTGTTAA+TGG	-	chr5.2:48497137-48497156	MS.gene65683:CDS	35.0%
	CAAGTTGTTAGAACTCACAA+AGG	-	chr5.2:48496621-48496640	MS.gene65683:CDS	35.0%
	CCCATTCTGAATAAACAGTT+TGG	+	chr5.2:48496537-48496556	None:intergenic	35.0%
	TTAAATGCAATAGTGCTGTG+TGG	-	chr5.2:48497102-48497121	MS.gene65683:CDS	35.0%
!	AGAGTATGGCAAATAGCTTT+TGG	-	chr5.2:48497071-48497090	MS.gene65683:CDS	35.0%
!	CATTTTTGTATTCATGCTGG+TGG	-	chr5.2:48496888-48496907	MS.gene65683:CDS	35.0%
!	CTCTTTCTTTTCACACTCAT+TGG	-	chr5.2:48496804-48496823	MS.gene65683:CDS	35.0%
!	GAGCATTTTTGTATTCATGC+TGG	-	chr5.2:48496885-48496904	MS.gene65683:CDS	35.0%
!	TCAAACGCTTGTTTGAAATC+AGG	+	chr5.2:48496867-48496886	None:intergenic	35.0%
!!	ATTTGGAACTCAACGCTTTT+CGG	+	chr5.2:48496093-48496112	None:intergenic	35.0%
	AACTCCTTCTTTGTCTGCTA+TGG	-	chr5.2:48496317-48496336	MS.gene65683:CDS	40.0%
	AAGAAGGAGTTGGAGAAAAG+AGG	+	chr5.2:48496308-48496327	None:intergenic	40.0%
	AAGGATCTTATGGCTATAGC+AGG	-	chr5.2:48496717-48496736	MS.gene65683:CDS	40.0%
	AGCGTTCGAGAATTCTCATT+TGG	+	chr5.2:48496110-48496129	None:intergenic	40.0%
	ATAAACCTGAAGAAGATCGC+GGG	+	chr5.2:48496433-48496452	None:intergenic	40.0%
	ATAGCAGACAAAGAAGGAGT+TGG	+	chr5.2:48496318-48496337	None:intergenic	40.0%
	ATCACCATAGCAGACAAAGA+AGG	+	chr5.2:48496324-48496343	None:intergenic	40.0%
	CTTCTGTAACTCATCGATCA+CGG	+	chr5.2:48496919-48496938	None:intergenic	40.0%
	CTTCTTCTTCGAAAACGCAA+CGG	+	chr5.2:48496665-48496684	None:intergenic	40.0%
	GAAGACAGAGAAGGTAAAGT+TGG	-	chr5.2:48496681-48496700	MS.gene65683:CDS	40.0%
	GATAAACCTGAAGAAGATCG+CGG	+	chr5.2:48496434-48496453	None:intergenic	40.0%
	GGATGAGAAGATAACAAGCT+CGG	+	chr5.2:48496217-48496236	None:intergenic	40.0%
	GTTCCATGAAAGTAGCGAAA+GGG	+	chr5.2:48496044-48496063	None:intergenic	40.0%
	GTTGTTAGAACTCACAAAGG+AGG	-	chr5.2:48496624-48496643	MS.gene65683:CDS	40.0%
	TATGTCCTTAGGTTTGAGTC+CGG	+	chr5.2:48496262-48496281	None:intergenic	40.0%
	TCAAAAGGAAGGATGAAGAG+AGG	-	chr5.2:48497044-48497063	MS.gene65683:CDS	40.0%
	TGAAGAGAGGTGATAGAGTA+TGG	-	chr5.2:48497057-48497076	MS.gene65683:CDS	40.0%
	TGTTCCATGAAAGTAGCGAA+AGG	+	chr5.2:48496045-48496064	None:intergenic	40.0%
	TTCGAAGAAGAAGACAGAGA+AGG	-	chr5.2:48496672-48496691	MS.gene65683:CDS	40.0%
	TTGGTTTAGGTGGGATGTAA+TGG	+	chr5.2:48496170-48496189	None:intergenic	40.0%
!	GATACCTTGTTTGCGAAAAC+CGG	-	chr5.2:48496240-48496259	MS.gene65683:CDS	40.0%
!	GCATTGCTCTTTCTTTTCCT+TGG	+	chr5.2:48496509-48496528	None:intergenic	40.0%
!	TTTCGCAAACAAGGTATCCA+TGG	+	chr5.2:48496238-48496257	None:intergenic	40.0%
!!	AAGAGTAGTTGTTCTGAAGC+AGG	+	chr5.2:48496789-48496808	None:intergenic	40.0%
!!	CTTCCATTGTTGGTTTAGGT+GGG	+	chr5.2:48496179-48496198	None:intergenic	40.0%
!!	GCTTCTTCCATTGTTGGTTT+AGG	+	chr5.2:48496183-48496202	None:intergenic	40.0%
!!	GCTTGTTATCTTCTCATCCA+TGG	-	chr5.2:48496218-48496237	MS.gene65683:CDS	40.0%
!!	TCTTCCATTGTTGGTTTAGG+TGG	+	chr5.2:48496180-48496199	None:intergenic	40.0%
!!	TGGCAAATAGCTTTTGGAAG+TGG	-	chr5.2:48497077-48497096	MS.gene65683:CDS	40.0%
!!	TTTGGAACTCAACGCTTTTC+GGG	+	chr5.2:48496092-48496111	None:intergenic	40.0%
	ATCAAGAGCTTCAACCTCTC+CGG	-	chr5.2:48496369-48496388	MS.gene65683:CDS	45.0%
	ATGAGAATTCTCGAACGCTC+TGG	-	chr5.2:48496111-48496130	MS.gene65683:CDS	45.0%
	CATCCCACCTAAACCAACAA+TGG	-	chr5.2:48496173-48496192	MS.gene65683:CDS	45.0%
	CGATCAATGCAATCTTCCCA+AGG	+	chr5.2:48497164-48497183	None:intergenic	45.0%
	CGTCTTGCTTCTTCCATTGT+TGG	+	chr5.2:48496189-48496208	None:intergenic	45.0%
	GAAAACCGGACTCAAACCTA+AGG	-	chr5.2:48496254-48496273	MS.gene65683:CDS	45.0%
	TCTTCCCAAGGTCCATTAAC+AGG	+	chr5.2:48497152-48497171	None:intergenic	45.0%
	TGAAGCAGGCATAATCGACT+AGG	+	chr5.2:48495999-48496018	None:intergenic	45.0%
!	AGACACCTGTTAATGGACCT+TGG	-	chr5.2:48497144-48497163	MS.gene65683:CDS	45.0%
!	AGTAATCGGTGGTTTGAAGC+AGG	+	chr5.2:48496013-48496032	None:intergenic	45.0%
!	GACACCTGTTAATGGACCTT+GGG	-	chr5.2:48497145-48497164	MS.gene65683:CDS	45.0%
!	TCTGAAGCAGGTAGAACAAG+AGG	+	chr5.2:48496777-48496796	None:intergenic	45.0%
!!	AGAATGACGCTGCATCGATT+CGG	-	chr5.2:48496975-48496994	MS.gene65683:CDS	45.0%
	AAAGGGACGCGGCAAGTAAT+CGG	+	chr5.2:48496027-48496046	None:intergenic	50.0%
	AGCTTCAACCTCTCCGGAAT+GGG	-	chr5.2:48496375-48496394	MS.gene65683:CDS	50.0%
	ATTCTCGAACGCTCTGGTCT+TGG	-	chr5.2:48496117-48496136	MS.gene65683:CDS	50.0%
	GCTATCTGCTGAACATGTCG+AGG	-	chr5.2:48496947-48496966	MS.gene65683:CDS	50.0%
	TGAAAGTAGCGAAAGGGACG+CGG	+	chr5.2:48496038-48496057	None:intergenic	50.0%
	TTTAGCCCGCGATCTTCTTC+AGG	-	chr5.2:48496425-48496444	MS.gene65683:CDS	50.0%
!	AAGATCGCGGGCTAAATCGA+TGG	+	chr5.2:48496421-48496440	None:intergenic	50.0%
!	CGTCCCTTTCGCTACTTTCA+TGG	-	chr5.2:48496038-48496057	MS.gene65683:CDS	50.0%
!	GAGTCCGGTTTTCGCAAACA+AGG	+	chr5.2:48496247-48496266	None:intergenic	50.0%
!!	GAATGACGCTGCATCGATTC+GGG	-	chr5.2:48496976-48496995	MS.gene65683:CDS	50.0%
!!	GGGATGTAATGGATTGCTGG+TGG	+	chr5.2:48496159-48496178	None:intergenic	50.0%
!!	GGTGGGATGTAATGGATTGC+TGG	+	chr5.2:48496162-48496181	None:intergenic	50.0%
!!!	CGCTTTTCGGGTTGTTCTTG+AGG	+	chr5.2:48496080-48496099	None:intergenic	50.0%
	GAGCTTCAACCTCTCCGGAA+TGG	-	chr5.2:48496374-48496393	MS.gene65683:CDS	55.0%
	TCGACTAGGTAGATTGCGCG+TGG	+	chr5.2:48495985-48496004	None:intergenic	55.0%
	ACCCGCGCTACATCCCATTC+CGG	+	chr5.2:48496391-48496410	None:intergenic	60.0%
	CGCTACATCCCATTCCGGAG+AGG	+	chr5.2:48496386-48496405	None:intergenic	60.0%
	CTCCGGAATGGGATGTAGCG+CGG	-	chr5.2:48496386-48496405	MS.gene65683:CDS	60.0%
	TCCGGAATGGGATGTAGCGC+GGG	-	chr5.2:48496387-48496406	MS.gene65683:CDS	60.0%
	GGGACGCGGCAAGTAATCGG+TGG	+	chr5.2:48496024-48496043	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	48495973	48497220	48495973	ID=MS.gene65683
chr5.2	mRNA	48495973	48497220	48495973	ID=MS.gene65683.t1;Parent=MS.gene65683
chr5.2	exon	48495973	48497220	48495973	ID=MS.gene65683.t1.exon1;Parent=MS.gene65683.t1
chr5.2	CDS	48495973	48497220	48495973	ID=cds.MS.gene65683.t1;Parent=MS.gene65683.t1

Gene Sequence

>MS.gene65683

ATGTTAAAGCCACGCGCAATCTACCTAGTCGATTATGCCTGCTTCAAACCACCGATTACTTGCCGCGTCCCTTTCGCTACTTTCATGGAACATTCAAGACTAATCCTCAAGAACAACCCGAAAAGCGTTGAGTTCCAAATGAGAATTCTCGAACGCTCTGGTCTTGGAGAAGAAACATGTTTGCCACCAGCAATCCATTACATCCCACCTAAACCAACAATGGAAGAAGCAAGACGCGAAGCCGAGCTTGTTATCTTCTCATCCATGGATACCTTGTTTGCGAAAACCGGACTCAAACCTAAGGACATAGACATTCTTATAGTCAATTGCAGCCTCTTTTCTCCAACTCCTTCTTTGTCTGCTATGGTGATCAACAAATACAAACTTAGAAGCAACATCAAGAGCTTCAACCTCTCCGGAATGGGATGTAGCGCGGGTTTAATCTCCATCGATTTAGCCCGCGATCTTCTTCAGGTTTATCCGAATTCAAACGCAGTTGTTGTGAGCACAGAAATCATTACTCCAAACTATTACCAAGGAAAAGAAAGAGCAATGCTTTTACCAAACTGTTTATTCAGAATGGGTGGTGCTGCTATTCTTTTATCAAACAAAAAAACAGAACACAAAAGAGCAAAATATAGATTGATGCAAGTTGTTAGAACTCACAAAGGAGGTGATGATAAAGCTTACCGTTGCGTTTTCGAAGAAGAAGACAGAGAAGGTAAAGTTGGAATTTCACTTCAAAAGGATCTTATGGCTATAGCAGGAGAAGCATTGAAATCCAATATAACAACTATTGGTCCTCTTGTTCTACCTGCTTCAGAACAACTACTCTTTCTTTTCACACTCATTGGAAAGAAAATCTTCAATCCTAAAATGAAGCCTTATATTCCTGATTTCAAACAAGCGTTTGAGCATTTTTGTATTCATGCTGGTGGACGTGCCGTGATCGATGAGTTACAGAAGAATCTTCAGCTATCTGCTGAACATGTCGAGGCTTCAAGAATGACGCTGCATCGATTCGGGAACACGTCTTCTTCATCTTTATGGTATGAATTGAACTATATTGAATCAAAAGGAAGGATGAAGAGAGGTGATAGAGTATGGCAAATAGCTTTTGGAAGTGGATTTAAATGCAATAGTGCTGTGTGGAAATGTAATAGAAGCATTAAGACACCTGTTAATGGACCTTGGGAAGATTGCATTGATCGCTATCCTGTTCATATTCCTGAGATTGTTAAGCTCTAA

Protein sequence

>MS.gene65683.t1

MLKPRAIYLVDYACFKPPITCRVPFATFMEHSRLILKNNPKSVEFQMRILERSGLGEETCLPPAIHYIPPKPTMEEARREAELVIFSSMDTLFAKTGLKPKDIDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGLISIDLARDLLQVYPNSNAVVVSTEIITPNYYQGKERAMLLPNCLFRMGGAAILLSNKKTEHKRAKYRLMQVVRTHKGGDDKAYRCVFEEEDREGKVGISLQKDLMAIAGEALKSNITTIGPLVLPASEQLLFLFTLIGKKIFNPKMKPYIPDFKQAFEHFCIHAGGRAVIDELQKNLQLSAEHVEASRMTLHRFGNTSSSSLWYELNYIESKGRMKRGDRVWQIAFGSGFKCNSAVWKCNRSIKTPVNGPWEDCIDRYPVHIPEIVKL