Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24098.t1 | PNY14915.1 | 85.7 | 307 | 34 | 3 | 4 | 305 | 3 | 304 | 8.20E-143 | 516.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24098.t1 | Q9SGS4 | 65.2 | 305 | 89 | 5 | 10 | 305 | 6 | 302 | 2.1e-102 | 373.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24098.t1 | A0A2K3PI43 | 85.7 | 307 | 34 | 3 | 4 | 305 | 3 | 304 | 5.9e-143 | 516.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene24098 | 0.802564 | 5.28E-49 | -1.69E-46 |
| MS.gene049150 | MS.gene24098 | 0.817386 | 3.39E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene24098 | 0.811353 | 7.31E-51 | -1.69E-46 |
| MS.gene049346 | MS.gene24098 | 0.820096 | 8.21E-53 | -1.69E-46 |
| MS.gene049351 | MS.gene24098 | 0.808405 | 3.15E-50 | -1.69E-46 |
| MS.gene049356 | MS.gene24098 | 0.851287 | 9.79E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene24098 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene049488 | MS.gene24098 | 0.817003 | 4.13E-52 | -1.69E-46 |
| MS.gene049661 | MS.gene24098 | 0.826255 | 3.00E-54 | -1.69E-46 |
| MS.gene049662 | MS.gene24098 | 0.824601 | 7.39E-54 | -1.69E-46 |
| MS.gene049681 | MS.gene24098 | 0.813509 | 2.47E-51 | -1.69E-46 |
| MS.gene049993 | MS.gene24098 | 0.822706 | 2.05E-53 | -1.69E-46 |
| MS.gene050122 | MS.gene24098 | 0.826375 | 2.81E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene24098 | 0.827455 | 1.55E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene24098 | 0.850745 | 1.39E-60 | -1.69E-46 |
| MS.gene050400 | MS.gene24098 | 0.800609 | 1.33E-48 | -1.69E-46 |
| MS.gene050406 | MS.gene24098 | 0.828672 | 7.89E-55 | -1.69E-46 |
| MS.gene050426 | MS.gene24098 | -0.854626 | 1.09E-61 | -1.69E-46 |
| MS.gene050464 | MS.gene24098 | 0.824911 | 6.24E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene24098 | 0.839819 | 1.26E-57 | -1.69E-46 |
| MS.gene050670 | MS.gene24098 | 0.899824 | 1.35E-77 | -1.69E-46 |
| MS.gene050812 | MS.gene24098 | 0.807495 | 4.92E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene24098 | 0.809137 | 2.20E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene24098 | 0.885391 | 8.47E-72 | -1.69E-46 |
| MS.gene051255 | MS.gene24098 | 0.80737 | 5.22E-50 | -1.69E-46 |
| MS.gene051360 | MS.gene24098 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene24098 | 0.816565 | 5.18E-52 | -1.69E-46 |
| MS.gene051525 | MS.gene24098 | 0.869377 | 3.24E-66 | -1.69E-46 |
| MS.gene051582 | MS.gene24098 | 0.840642 | 7.69E-58 | -1.69E-46 |
| MS.gene051588 | MS.gene24098 | 0.800436 | 1.44E-48 | -1.69E-46 |
| MS.gene051605 | MS.gene24098 | 0.806716 | 7.19E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene24098 | 0.811346 | 7.33E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene24098 | -0.887591 | 1.25E-72 | -1.69E-46 |
| MS.gene052077 | MS.gene24098 | 0.856606 | 2.87E-62 | -1.69E-46 |
| MS.gene052128 | MS.gene24098 | -0.801129 | 1.04E-48 | -1.69E-46 |
| MS.gene052134 | MS.gene24098 | 0.832429 | 9.50E-56 | -1.69E-46 |
| MS.gene052135 | MS.gene24098 | 0.844521 | 7.18E-59 | -1.69E-46 |
| MS.gene052136 | MS.gene24098 | 0.833451 | 5.29E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene24098 | 0.883077 | 6.09E-71 | -1.69E-46 |
| MS.gene052253 | MS.gene24098 | 0.895012 | 1.44E-75 | -1.69E-46 |
| MS.gene052255 | MS.gene24098 | 0.823477 | 1.36E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene24098 | 0.853003 | 3.18E-61 | -1.69E-46 |
| MS.gene052322 | MS.gene24098 | 0.814167 | 1.77E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene24098 | 0.876187 | 1.70E-68 | -1.69E-46 |
| MS.gene052346 | MS.gene24098 | 0.819159 | 1.34E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene24098 | 0.867365 | 1.44E-65 | -1.69E-46 |
| MS.gene052421 | MS.gene24098 | 0.827811 | 1.27E-54 | -1.69E-46 |
| MS.gene052481 | MS.gene24098 | 0.825108 | 5.61E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene24098 | 0.849312 | 3.51E-60 | -1.69E-46 |
| MS.gene052501 | MS.gene24098 | 0.816962 | 4.22E-52 | -1.69E-46 |
| MS.gene052505 | MS.gene24098 | 0.831534 | 1.58E-55 | -1.69E-46 |
| MS.gene052758 | MS.gene24098 | 0.808998 | 2.35E-50 | -1.69E-46 |
| MS.gene052982 | MS.gene24098 | 0.835493 | 1.63E-56 | -1.69E-46 |
| MS.gene053169 | MS.gene24098 | 0.834716 | 2.55E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene24098 | 0.831889 | 1.29E-55 | -1.69E-46 |
| MS.gene053330 | MS.gene24098 | 0.826686 | 2.37E-54 | -1.69E-46 |
| MS.gene053557 | MS.gene24098 | 0.813372 | 2.65E-51 | -1.69E-46 |
| MS.gene053758 | MS.gene24098 | 0.835687 | 1.45E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene24098 | 0.834505 | 2.88E-56 | -1.69E-46 |
| MS.gene053873 | MS.gene24098 | 0.826204 | 3.08E-54 | -1.69E-46 |
| MS.gene053928 | MS.gene24098 | 0.849229 | 3.70E-60 | -1.69E-46 |
| MS.gene054010 | MS.gene24098 | 0.816647 | 4.97E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene24098 | 0.817487 | 3.21E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene24098 | 0.817481 | 3.22E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene24098 | 0.809951 | 1.47E-50 | -1.69E-46 |
| MS.gene054102 | MS.gene24098 | 0.847446 | 1.15E-59 | -1.69E-46 |
| MS.gene054110 | MS.gene24098 | 0.820811 | 5.63E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene24098 | 0.81869 | 1.72E-52 | -1.69E-46 |
| MS.gene05413 | MS.gene24098 | 0.80822 | 3.45E-50 | -1.69E-46 |
| MS.gene054173 | MS.gene24098 | 0.808116 | 3.63E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene24098 | 0.883721 | 3.53E-71 | -1.69E-46 |
| MS.gene054349 | MS.gene24098 | 0.890683 | 7.89E-74 | -1.69E-46 |
| MS.gene054556 | MS.gene24098 | 0.866275 | 3.21E-65 | -1.69E-46 |
| MS.gene054719 | MS.gene24098 | 0.85558 | 5.74E-62 | -1.69E-46 |
| MS.gene054918 | MS.gene24098 | 0.82472 | 6.93E-54 | -1.69E-46 |
| MS.gene054920 | MS.gene24098 | 0.809329 | 2.00E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene24098 | 0.810383 | 1.18E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene24098 | 0.917877 | 3.11E-86 | -1.69E-46 |
| MS.gene055277 | MS.gene24098 | 0.821135 | 4.74E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene24098 | 0.884547 | 1.75E-71 | -1.69E-46 |
| MS.gene055428 | MS.gene24098 | 0.800747 | 1.25E-48 | -1.69E-46 |
| MS.gene055539 | MS.gene24098 | 0.853864 | 1.80E-61 | -1.69E-46 |
| MS.gene05553 | MS.gene24098 | 0.88544 | 8.12E-72 | -1.69E-46 |
| MS.gene055564 | MS.gene24098 | 0.837741 | 4.35E-57 | -1.69E-46 |
| MS.gene055604 | MS.gene24098 | 0.852812 | 3.61E-61 | -1.69E-46 |
| MS.gene055741 | MS.gene24098 | 0.852077 | 5.85E-61 | -1.69E-46 |
| MS.gene055744 | MS.gene24098 | 0.863204 | 2.94E-64 | -1.69E-46 |
| MS.gene055909 | MS.gene24098 | 0.82189 | 3.17E-53 | -1.69E-46 |
| MS.gene055911 | MS.gene24098 | 0.828975 | 6.67E-55 | -1.69E-46 |
| MS.gene05594 | MS.gene24098 | 0.844886 | 5.73E-59 | -1.69E-46 |
| MS.gene05602 | MS.gene24098 | 0.806845 | 6.75E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene24098 | 0.843909 | 1.05E-58 | -1.69E-46 |
| MS.gene056337 | MS.gene24098 | 0.836982 | 6.80E-57 | -1.69E-46 |
| MS.gene056407 | MS.gene24098 | 0.865714 | 4.83E-65 | -1.69E-46 |
| MS.gene056511 | MS.gene24098 | 0.849498 | 3.11E-60 | -1.69E-46 |
| MS.gene056514 | MS.gene24098 | 0.861668 | 8.73E-64 | -1.69E-46 |
| MS.gene056612 | MS.gene24098 | 0.801084 | 1.06E-48 | -1.69E-46 |
| MS.gene05663 | MS.gene24098 | 0.805721 | 1.16E-49 | -1.69E-46 |
| MS.gene05664 | MS.gene24098 | 0.814334 | 1.62E-51 | -1.69E-46 |
| MS.gene056659 | MS.gene24098 | 0.895334 | 1.06E-75 | -1.69E-46 |
| MS.gene05666 | MS.gene24098 | 0.805987 | 1.02E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene24098 | 0.874901 | 4.70E-68 | -1.69E-46 |
| MS.gene056782 | MS.gene24098 | 0.804658 | 1.94E-49 | -1.69E-46 |
| MS.gene056796 | MS.gene24098 | 0.815078 | 1.11E-51 | -1.69E-46 |
| MS.gene056877 | MS.gene24098 | 0.807537 | 4.82E-50 | -1.69E-46 |
| MS.gene056899 | MS.gene24098 | -0.834448 | 2.98E-56 | -1.69E-46 |
| MS.gene056902 | MS.gene24098 | 0.803802 | 2.93E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene24098 | 0.880514 | 5.16E-70 | -1.69E-46 |
| MS.gene057124 | MS.gene24098 | 0.873886 | 1.04E-67 | -1.69E-46 |
| MS.gene057207 | MS.gene24098 | 0.802914 | 4.47E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene24098 | 0.859558 | 3.81E-63 | -1.69E-46 |
| MS.gene057448 | MS.gene24098 | 0.82203 | 2.95E-53 | -1.69E-46 |
| MS.gene057450 | MS.gene24098 | 0.810852 | 9.38E-51 | -1.69E-46 |
| MS.gene057476 | MS.gene24098 | 0.843268 | 1.56E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene24098 | 0.90673 | 1.09E-80 | -1.69E-46 |
| MS.gene057823 | MS.gene24098 | 0.898197 | 6.72E-77 | -1.69E-46 |
| MS.gene057824 | MS.gene24098 | 0.837947 | 3.85E-57 | -1.69E-46 |
| MS.gene057825 | MS.gene24098 | 0.883322 | 4.95E-71 | -1.69E-46 |
| MS.gene057826 | MS.gene24098 | 0.835567 | 1.56E-56 | -1.69E-46 |
| MS.gene057888 | MS.gene24098 | 0.816155 | 6.40E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene24098 | 0.85936 | 4.36E-63 | -1.69E-46 |
| MS.gene058007 | MS.gene24098 | 0.845099 | 5.02E-59 | -1.69E-46 |
| MS.gene058082 | MS.gene24098 | 0.802255 | 6.12E-49 | -1.69E-46 |
| MS.gene058090 | MS.gene24098 | 0.800538 | 1.38E-48 | -1.69E-46 |
| MS.gene05811 | MS.gene24098 | 0.81701 | 4.12E-52 | -1.69E-46 |
| MS.gene058120 | MS.gene24098 | 0.801253 | 9.82E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene24098 | 0.856639 | 2.81E-62 | -1.69E-46 |
| MS.gene058233 | MS.gene24098 | 0.833892 | 4.11E-56 | -1.69E-46 |
| MS.gene058276 | MS.gene24098 | 0.80132 | 9.52E-49 | -1.69E-46 |
| MS.gene058365 | MS.gene24098 | 0.842764 | 2.12E-58 | -1.69E-46 |
| MS.gene058376 | MS.gene24098 | 0.818275 | 2.13E-52 | -1.69E-46 |
| MS.gene058444 | MS.gene24098 | 0.826833 | 2.18E-54 | -1.69E-46 |
| MS.gene058660 | MS.gene24098 | 0.826233 | 3.04E-54 | -1.69E-46 |
| MS.gene058700 | MS.gene24098 | 0.851083 | 1.12E-60 | -1.69E-46 |
| MS.gene058710 | MS.gene24098 | 0.866114 | 3.61E-65 | -1.69E-46 |
| MS.gene05886 | MS.gene24098 | 0.870285 | 1.64E-66 | -1.69E-46 |
| MS.gene058928 | MS.gene24098 | 0.849113 | 3.98E-60 | -1.69E-46 |
| MS.gene058948 | MS.gene24098 | 0.855986 | 4.36E-62 | -1.69E-46 |
| MS.gene058949 | MS.gene24098 | 0.87863 | 2.40E-69 | -1.69E-46 |
| MS.gene058995 | MS.gene24098 | 0.81871 | 1.70E-52 | -1.69E-46 |
| MS.gene059011 | MS.gene24098 | 0.886299 | 3.86E-72 | -1.69E-46 |
| MS.gene05907 | MS.gene24098 | 0.859137 | 5.09E-63 | -1.69E-46 |
| MS.gene059082 | MS.gene24098 | 0.834569 | 2.78E-56 | -1.69E-46 |
| MS.gene059141 | MS.gene24098 | 0.832455 | 9.36E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene24098 | 0.853736 | 1.96E-61 | -1.69E-46 |
| MS.gene059294 | MS.gene24098 | 0.828582 | 8.30E-55 | -1.69E-46 |
| MS.gene059366 | MS.gene24098 | 0.822057 | 2.90E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene24098 | 0.907818 | 3.36E-81 | -1.69E-46 |
| MS.gene059455 | MS.gene24098 | 0.800166 | 1.64E-48 | -1.69E-46 |
| MS.gene059462 | MS.gene24098 | 0.842257 | 2.89E-58 | -1.69E-46 |
| MS.gene059597 | MS.gene24098 | 0.823284 | 1.51E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene24098 | 0.80626 | 8.97E-50 | -1.69E-46 |
| MS.gene059676 | MS.gene24098 | 0.826139 | 3.20E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene24098 | 0.878267 | 3.22E-69 | -1.69E-46 |
| MS.gene059692 | MS.gene24098 | 0.875195 | 3.73E-68 | -1.69E-46 |
| MS.gene059695 | MS.gene24098 | 0.877383 | 6.57E-69 | -1.69E-46 |
| MS.gene059807 | MS.gene24098 | 0.825404 | 4.78E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene24098 | 0.83181 | 1.35E-55 | -1.69E-46 |
| MS.gene059876 | MS.gene24098 | 0.833154 | 6.28E-56 | -1.69E-46 |
| MS.gene060038 | MS.gene24098 | 0.814098 | 1.83E-51 | -1.69E-46 |
| MS.gene060247 | MS.gene24098 | 0.82297 | 1.78E-53 | -1.69E-46 |
| MS.gene060372 | MS.gene24098 | 0.818553 | 1.85E-52 | -1.69E-46 |
| MS.gene06037 | MS.gene24098 | 0.82435 | 8.47E-54 | -1.69E-46 |
| MS.gene060403 | MS.gene24098 | 0.867015 | 1.87E-65 | -1.69E-46 |
| MS.gene060407 | MS.gene24098 | 0.824355 | 8.44E-54 | -1.69E-46 |
| MS.gene060804 | MS.gene24098 | 0.806008 | 1.01E-49 | -1.69E-46 |
| MS.gene060835 | MS.gene24098 | 0.82629 | 2.94E-54 | -1.69E-46 |
| MS.gene060837 | MS.gene24098 | 0.823386 | 1.42E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene24098 | 0.870474 | 1.42E-66 | -1.69E-46 |
| MS.gene061051 | MS.gene24098 | -0.82388 | 1.09E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene24098 | 0.885318 | 9.02E-72 | -1.69E-46 |
| MS.gene061159 | MS.gene24098 | 0.831338 | 1.77E-55 | -1.69E-46 |
| MS.gene061214 | MS.gene24098 | 0.80901 | 2.34E-50 | -1.69E-46 |
| MS.gene061215 | MS.gene24098 | 0.807453 | 5.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24098.t1 | MTR_1g026125 | 90.066 | 302 | 16 | 4 | 10 | 305 | 5 | 298 | 0.0 | 534 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24098.t1 | AT1G76080 | 64.331 | 314 | 91 | 6 | 1 | 305 | 1 | 302 | 2.50e-136 | 388 |
Find 47 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTGAGATATACCCTTTCT+TGG | 0.236936 | 1.2:+178525 | MS.gene24098:CDS |
| TGTGGTAGGTATGTTGGTTC+AGG | 0.274596 | 1.2:+179030 | MS.gene24098:CDS |
| TGTTGAGTTTCTTCTTGTTA+TGG | 0.292877 | 1.2:+178573 | MS.gene24098:CDS |
| GAGGGATATGGAAGTTGTTC+AGG | 0.305188 | 1.2:+178972 | MS.gene24098:CDS |
| ACATCACCAACAAGCATATC+TGG | 0.321339 | 1.2:-178709 | None:intergenic |
| GTTGAGTTTCTTCTTGTTAT+GGG | 0.323733 | 1.2:+178574 | MS.gene24098:CDS |
| TGAGGGTTTGCATATGAAAT+TGG | 0.324749 | 1.2:-178218 | None:intergenic |
| AGCTGCTGTGCCTGTTGCTT+TGG | 0.336628 | 1.2:-178371 | None:intergenic |
| GAATGAAGGGTAAGGAAGTT+AGG | 0.357510 | 1.2:-178265 | None:intergenic |
| AAGCTTATTGTTCTTGATGT+TGG | 0.379209 | 1.2:+178823 | MS.gene24098:CDS |
| TACTACGGTGATAACCATTC+AGG | 0.418710 | 1.2:+178736 | MS.gene24098:CDS |
| CATCGTTACATGTTCGGCTT+AGG | 0.421411 | 1.2:-178552 | None:intergenic |
| GTGGTAGGTATGTTGGTTCA+GGG | 0.432018 | 1.2:+179031 | MS.gene24098:CDS |
| AACTCATTGGTGAAATTCTC+AGG | 0.435220 | 1.2:+179061 | MS.gene24098:CDS |
| ATTAGAGATGGTAAGATTTG+TGG | 0.439626 | 1.2:+179012 | MS.gene24098:CDS |
| GAAATTGAAGGAGGGATTGA+GGG | 0.440827 | 1.2:-178235 | None:intergenic |
| CGGCTTAGGTCAACCAAGAA+AGG | 0.444183 | 1.2:-178538 | None:intergenic |
| AAGATTTGTGGTAGGTATGT+TGG | 0.445880 | 1.2:+179024 | MS.gene24098:CDS |
| TTAGGATGAGTTGAAATTGA+AGG | 0.445917 | 1.2:-178247 | None:intergenic |
| ACATTGTGGACCATGTGTTA+AGG | 0.454327 | 1.2:+178852 | MS.gene24098:CDS |
| TGAAATTGAAGGAGGGATTG+AGG | 0.455754 | 1.2:-178236 | None:intergenic |
| GTTGTTATCACCAAAGCAAC+AGG | 0.459253 | 1.2:+178361 | MS.gene24098:CDS |
| ACTCAACATCGTTACATGTT+CGG | 0.469979 | 1.2:-178558 | None:intergenic |
| ATTCATGAAGAAGAAGCTAT+TGG | 0.470180 | 1.2:+178685 | MS.gene24098:CDS |
| AAGAAGAACGAAGAGAATGA+AGG | 0.480454 | 1.2:-178279 | None:intergenic |
| AGGTATGTTGGTTCAGGGAA+AGG | 0.482216 | 1.2:+179036 | MS.gene24098:CDS |
| AGAAGAACGAAGAGAATGAA+GGG | 0.507243 | 1.2:-178278 | None:intergenic |
| AACGAAGAGAATGAAGGGTA+AGG | 0.514490 | 1.2:-178273 | None:intergenic |
| AGAACTCTGCAAAAGAGAAA+AGG | 0.515029 | 1.2:+178618 | MS.gene24098:CDS |
| GGATGAGTTGAAATTGAAGG+AGG | 0.516103 | 1.2:-178244 | None:intergenic |
| AATAAGTAACACGAACTCCT+TGG | 0.520146 | 1.2:-179086 | None:intergenic |
| TCAGGGAAAGGTGAACTCAT+TGG | 0.523543 | 1.2:+179048 | MS.gene24098:CDS |
| CTTGACAGCTTAATCACTGT+CGG | 0.540890 | 1.2:-178880 | None:intergenic |
| GATGAGTTGAAATTGAAGGA+GGG | 0.552894 | 1.2:-178243 | None:intergenic |
| AGATGTTGAGAAACTTATTG+AGG | 0.553117 | 1.2:+178783 | MS.gene24098:CDS |
| ATTCATTCTACTTCTCGGGT+AGG | 0.557229 | 1.2:+178325 | MS.gene24098:CDS |
| ATTGGTCCAGATATGCTTGT+TGG | 0.557827 | 1.2:+178703 | MS.gene24098:CDS |
| GGCTTAGGTCAACCAAGAAA+GGG | 0.570139 | 1.2:-178537 | None:intergenic |
| GTTGGTGATGTGTTATACTA+CGG | 0.591943 | 1.2:+178721 | MS.gene24098:CDS |
| GTGAGTGATTGTAGTAGCCA+TGG | 0.593491 | 1.2:-178191 | None:intergenic |
| GAAGTTGTACTACACCTGAA+TGG | 0.613386 | 1.2:-178750 | None:intergenic |
| TGAGTGATTGTAGTAGCCAT+GGG | 0.617890 | 1.2:-178190 | None:intergenic |
| GAGATGGTAAGATTTGTGGT+AGG | 0.631571 | 1.2:+179016 | MS.gene24098:CDS |
| GATGTTGGTCTTAAACATTG+TGG | 0.644010 | 1.2:+178838 | MS.gene24098:CDS |
| GGTGAAATTCTCAGGTACCA+AGG | 0.658840 | 1.2:+179069 | MS.gene24098:CDS |
| GTCGGATAAACCTTAACACA+TGG | 0.666870 | 1.2:-178862 | None:intergenic |
| CTCTTGTAGAAAGTGAAGTG+TGG | 0.692063 | 1.2:-178652 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAAATTCATTCTACTTCTC+GGG | + | chr1.2:178321-178340 | MS.gene24098:CDS | 25.0% |
| ! | CAAAAATTCATTCTACTTCT+CGG | + | chr1.2:178320-178339 | MS.gene24098:CDS | 25.0% |
| ! | TCTCTAATGAACAAAAAAGT+AGG | - | chr1.2:179000-179019 | None:intergenic | 25.0% |
| !!! | ACTTTTTTGTTCATTAGAGA+TGG | + | chr1.2:179000-179019 | MS.gene24098:CDS | 25.0% |
| !!! | ATGATACTGTTATTTTTGCA+AGG | + | chr1.2:178911-178930 | MS.gene24098:CDS | 25.0% |
| AAGCTTATTGTTCTTGATGT+TGG | + | chr1.2:178823-178842 | MS.gene24098:CDS | 30.0% | |
| AGATGTTGAGAAACTTATTG+AGG | + | chr1.2:178783-178802 | MS.gene24098:CDS | 30.0% | |
| ATTAGAGATGGTAAGATTTG+TGG | + | chr1.2:179012-179031 | MS.gene24098:CDS | 30.0% | |
| ATTCATGAAGAAGAAGCTAT+TGG | + | chr1.2:178685-178704 | MS.gene24098:CDS | 30.0% | |
| TGTTGAGTTTCTTCTTGTTA+TGG | + | chr1.2:178573-178592 | MS.gene24098:CDS | 30.0% | |
| ! | ATAGTTGTATGCAGTTTTTG+AGG | + | chr1.2:178953-178972 | MS.gene24098:CDS | 30.0% |
| ! | CGAGAAGTAGAATGAATTTT+TGG | - | chr1.2:178322-178341 | None:intergenic | 30.0% |
| ! | GTTGAGTTTCTTCTTGTTAT+GGG | + | chr1.2:178574-178593 | MS.gene24098:CDS | 30.0% |
| ! | TAGTTGTATGCAGTTTTTGA+GGG | + | chr1.2:178954-178973 | MS.gene24098:CDS | 30.0% |
| ! | TTAGGATGAGTTGAAATTGA+AGG | - | chr1.2:178250-178269 | None:intergenic | 30.0% |
| !!! | GTTATTTTTGCAAGGATGAA+TGG | + | chr1.2:178919-178938 | MS.gene24098:CDS | 30.0% |
| AACTCATTGGTGAAATTCTC+AGG | + | chr1.2:179061-179080 | MS.gene24098:CDS | 35.0% | |
| AAGAAGAACGAAGAGAATGA+AGG | - | chr1.2:178282-178301 | None:intergenic | 35.0% | |
| AAGATTTGTGGTAGGTATGT+TGG | + | chr1.2:179024-179043 | MS.gene24098:CDS | 35.0% | |
| AATAAGTAACACGAACTCCT+TGG | - | chr1.2:179089-179108 | None:intergenic | 35.0% | |
| ACTCAACATCGTTACATGTT+CGG | - | chr1.2:178561-178580 | None:intergenic | 35.0% | |
| AGAACTCTGCAAAAGAGAAA+AGG | + | chr1.2:178618-178637 | MS.gene24098:CDS | 35.0% | |
| AGAAGAACGAAGAGAATGAA+GGG | - | chr1.2:178281-178300 | None:intergenic | 35.0% | |
| CATTGAGATATACCCTTTCT+TGG | + | chr1.2:178525-178544 | MS.gene24098:CDS | 35.0% | |
| TGAGGGTTTGCATATGAAAT+TGG | - | chr1.2:178221-178240 | None:intergenic | 35.0% | |
| ! | GATGAGTTGAAATTGAAGGA+GGG | - | chr1.2:178246-178265 | None:intergenic | 35.0% |
| !! | GATGTTGGTCTTAAACATTG+TGG | + | chr1.2:178838-178857 | MS.gene24098:CDS | 35.0% |
| !! | GTTGGTGATGTGTTATACTA+CGG | + | chr1.2:178721-178740 | MS.gene24098:CDS | 35.0% |
| !! | TATGCAGTTTTTGAGGGATA+TGG | + | chr1.2:178960-178979 | MS.gene24098:CDS | 35.0% |
| AACGAAGAGAATGAAGGGTA+AGG | - | chr1.2:178276-178295 | None:intergenic | 40.0% | |
| ACATCACCAACAAGCATATC+TGG | - | chr1.2:178712-178731 | None:intergenic | 40.0% | |
| ACATTGTGGACCATGTGTTA+AGG | + | chr1.2:178852-178871 | MS.gene24098:CDS | 40.0% | |
| ATTCATTCTACTTCTCGGGT+AGG | + | chr1.2:178325-178344 | MS.gene24098:CDS | 40.0% | |
| ATTGGTCCAGATATGCTTGT+TGG | + | chr1.2:178703-178722 | MS.gene24098:CDS | 40.0% | |
| CTCTTGTAGAAAGTGAAGTG+TGG | - | chr1.2:178655-178674 | None:intergenic | 40.0% | |
| CTTGACAGCTTAATCACTGT+CGG | - | chr1.2:178883-178902 | None:intergenic | 40.0% | |
| GAAATTGAAGGAGGGATTGA+GGG | - | chr1.2:178238-178257 | None:intergenic | 40.0% | |
| GAAGTTGTACTACACCTGAA+TGG | - | chr1.2:178753-178772 | None:intergenic | 40.0% | |
| GAATGAAGGGTAAGGAAGTT+AGG | - | chr1.2:178268-178287 | None:intergenic | 40.0% | |
| GAGATGGTAAGATTTGTGGT+AGG | + | chr1.2:179016-179035 | MS.gene24098:CDS | 40.0% | |
| GTCGGATAAACCTTAACACA+TGG | - | chr1.2:178865-178884 | None:intergenic | 40.0% | |
| GTTGTTATCACCAAAGCAAC+AGG | + | chr1.2:178361-178380 | MS.gene24098:CDS | 40.0% | |
| TACTACGGTGATAACCATTC+AGG | + | chr1.2:178736-178755 | MS.gene24098:CDS | 40.0% | |
| TGAAATTGAAGGAGGGATTG+AGG | - | chr1.2:178239-178258 | None:intergenic | 40.0% | |
| ! | GGATGAGTTGAAATTGAAGG+AGG | - | chr1.2:178247-178266 | None:intergenic | 40.0% |
| CATCGTTACATGTTCGGCTT+AGG | - | chr1.2:178555-178574 | None:intergenic | 45.0% | |
| GAGGGATATGGAAGTTGTTC+AGG | + | chr1.2:178972-178991 | MS.gene24098:CDS | 45.0% | |
| GGCTTAGGTCAACCAAGAAA+GGG | - | chr1.2:178540-178559 | None:intergenic | 45.0% | |
| GGTGAAATTCTCAGGTACCA+AGG | + | chr1.2:179069-179088 | MS.gene24098:CDS | 45.0% | |
| TCAGGGAAAGGTGAACTCAT+TGG | + | chr1.2:179048-179067 | MS.gene24098:CDS | 45.0% | |
| !! | AGGTATGTTGGTTCAGGGAA+AGG | + | chr1.2:179036-179055 | MS.gene24098:CDS | 45.0% |
| !! | GTGGTAGGTATGTTGGTTCA+GGG | + | chr1.2:179031-179050 | MS.gene24098:CDS | 45.0% |
| !! | TGTGGTAGGTATGTTGGTTC+AGG | + | chr1.2:179030-179049 | MS.gene24098:CDS | 45.0% |
| CGGCTTAGGTCAACCAAGAA+AGG | - | chr1.2:178541-178560 | None:intergenic | 50.0% | |
| ! | AGCTGCTGTGCCTGTTGCTT+TGG | - | chr1.2:178374-178393 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 178193 | 179110 | 178193 | ID=MS.gene24098 |
| chr1.2 | mRNA | 178193 | 179110 | 178193 | ID=MS.gene24098.t1;Parent=MS.gene24098 |
| chr1.2 | exon | 178193 | 179110 | 178193 | ID=MS.gene24098.t1.exon1;Parent=MS.gene24098.t1 |
| chr1.2 | CDS | 178193 | 179110 | 178193 | ID=cds.MS.gene24098.t1;Parent=MS.gene24098.t1 |
| Gene Sequence |
| Protein sequence |
