AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29105


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29105.t1 MTR_3g073140 98.795 166 2 0 1 166 1 166 1.72e-119 334
MS.gene29105.t1 MTR_4g081120 47.727 132 69 0 21 152 34 165 6.99e-35 120
MS.gene29105.t1 MTR_4g081120 53.000 100 47 0 53 152 46 145 2.84e-34 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29105.t1 AT5G14320 70.513 156 41 2 1 153 1 154 1.42e-76 226
MS.gene29105.t1 AT5G14320 62.069 116 39 2 1 113 1 114 2.91e-44 142
MS.gene29105.t1 AT1G77750 48.387 124 64 0 42 165 27 150 1.66e-37 126

Find 38 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGTCCTGCAGTTATTCT+TGG 0.227656 3.2:+65972258 None:intergenic
AATTCAATTCTCTTGTTGTT+TGG 0.264258 3.2:+65972628 None:intergenic
CAGAGGAATAAGGCATATTC+AGG 0.285946 3.2:-65972305 MS.gene29105:CDS
AATTGTGTTACCTTTGACTT+TGG 0.293488 3.2:+65972757 None:intergenic
GGTAAGAAGGTTACTGTTGC+TGG 0.360552 3.2:-65972232 MS.gene29105:CDS
GACTTTGGAGTGTTGAGAAC+TGG 0.363379 3.2:+65972772 None:intergenic
TTCAGTGTTACAGAGGAATA+AGG 0.379843 3.2:-65972315 MS.gene29105:CDS
AAGAAGAGCTTATTAGTCTC+AGG 0.387619 3.2:-65972510 MS.gene29105:CDS
ATACATGACTGAAGGAGATT+TGG 0.390225 3.2:-65972473 MS.gene29105:intron
GGAGAAGTGACCATGGCCTT+TGG 0.397280 3.2:+65972810 None:intergenic
GATTCTAACTGATATCAGTA+TGG 0.462554 3.2:-65972560 MS.gene29105:CDS
TTGCAGTACATTCATGGAGT+TGG 0.465952 3.2:-65972601 MS.gene29105:CDS
TTAATGCACTTAATATAAAG+AGG 0.487114 3.2:-65972348 MS.gene29105:CDS
AACTGGGAAAGAAACTCGAT+TGG 0.508056 3.2:+65972789 None:intergenic
GAATAACTGCAGGACATTGA+AGG 0.525777 3.2:-65972254 MS.gene29105:CDS
CAGGACATTGAAGGGTAAGA+AGG 0.533951 3.2:-65972245 MS.gene29105:CDS
ATTGTGTGCGTTCGTGTTGG+TGG 0.534997 3.2:-65972661 MS.gene29105:CDS
AATAACTGCAGGACATTGAA+GGG 0.550619 3.2:-65972253 MS.gene29105:CDS
ATTGAGAGTGAAGGTGCCAC+AGG 0.552065 3.2:+65972838 None:intergenic
ACTTTGGAGTGTTGAGAACT+GGG 0.555353 3.2:+65972773 None:intergenic
AACATTGTGTGCGTTCGTGT+TGG 0.564683 3.2:-65972664 MS.gene29105:CDS
TGATTGAATTGTAGGTTCAG+AGG 0.566135 3.2:-65972690 MS.gene29105:intron
TCAATCATCTCCAAAGGCCA+TGG 0.572793 3.2:-65972820 MS.gene29105:CDS
GTGCGTTCGTGTTGGTGGTG+TGG 0.576835 3.2:-65972656 MS.gene29105:CDS
TTGGTACGCTGTCCTCTACA+AGG 0.578588 3.2:+65972277 None:intergenic
AGAGGAATAAGGCATATTCA+GGG 0.580912 3.2:-65972304 MS.gene29105:CDS
TCTCAACACTCCAAAGTCAA+AGG 0.589008 3.2:-65972767 MS.gene29105:intron
GTTAACAAATACATGACTGA+AGG 0.596392 3.2:-65972481 MS.gene29105:CDS
TATGGAGAATAAAGTTACAA+AGG 0.597785 3.2:-65972542 MS.gene29105:CDS
AAAGAAATTCAGTGTTACAG+AGG 0.605461 3.2:-65972322 MS.gene29105:CDS
TCACTCTCAATCATCTCCAA+AGG 0.619107 3.2:-65972826 MS.gene29105:CDS
ATTCAGGGACTTCCTTGTAG+AGG 0.621863 3.2:-65972289 MS.gene29105:CDS
AGTACATTCATGGAGTTGGA+AGG 0.622828 3.2:-65972597 MS.gene29105:CDS
AGCGTACCAAGAATAACTGC+AGG 0.623820 3.2:-65972264 MS.gene29105:CDS
AGAAGAGCTTATTAGTCTCA+GGG 0.625713 3.2:-65972509 MS.gene29105:CDS
CTCGATTGGAGAAGTGACCA+TGG 0.660409 3.2:+65972803 None:intergenic
TTGGAGATGATTGAGAGTGA+AGG 0.661623 3.2:+65972829 None:intergenic
ACAAACACTCTCAATGCCTG+TGG 0.676701 3.2:-65972854 MS.gene29105:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTTTGTAATTGTAA+TGG - chr3.2:65972674-65972693 MS.gene29105:CDS 15.0%
!! TTAATGCACTTAATATAAAG+AGG - chr3.2:65972727-65972746 MS.gene29105:intron 20.0%
! TATGGAGAATAAAGTTACAA+AGG - chr3.2:65972533-65972552 MS.gene29105:CDS 25.0%
!!! AATTCAATTCTCTTGTTGTT+TGG + chr3.2:65972450-65972469 None:intergenic 25.0%
AAAGAAATTCAGTGTTACAG+AGG - chr3.2:65972753-65972772 MS.gene29105:intron 30.0%
AAGTCAAAGGTAACACAATT+AGG - chr3.2:65972321-65972340 MS.gene29105:CDS 30.0%
AGTCAAAGGTAACACAATTA+GGG - chr3.2:65972322-65972341 MS.gene29105:CDS 30.0%
ATCAGAATCAGAATGGAATT+TGG + chr3.2:65972369-65972388 None:intergenic 30.0%
CAATTCAATCAGAATCAGAA+TGG + chr3.2:65972376-65972395 None:intergenic 30.0%
GATTCTAACTGATATCAGTA+TGG - chr3.2:65972515-65972534 MS.gene29105:CDS 30.0%
GTTAACAAATACATGACTGA+AGG - chr3.2:65972594-65972613 MS.gene29105:CDS 30.0%
TCTGATTCTGATTGAATTGT+AGG - chr3.2:65972377-65972396 MS.gene29105:intron 30.0%
TTGTTTGTGAATTTCAGAGA+AGG - chr3.2:65972703-65972722 MS.gene29105:intron 30.0%
! AATTGTGTTACCTTTGACTT+TGG + chr3.2:65972321-65972340 None:intergenic 30.0%
AATAACTGCAGGACATTGAA+GGG - chr3.2:65972822-65972841 MS.gene29105:CDS 35.0%
AGAGGAATAAGGCATATTCA+GGG - chr3.2:65972771-65972790 MS.gene29105:CDS 35.0%
ATACATGACTGAAGGAGATT+TGG - chr3.2:65972602-65972621 MS.gene29105:CDS 35.0%
TGATTGAATTGTAGGTTCAG+AGG - chr3.2:65972385-65972404 MS.gene29105:intron 35.0%
TTCAGTGTTACAGAGGAATA+AGG - chr3.2:65972760-65972779 MS.gene29105:intron 35.0%
! AAGAAGAGCTTATTAGTCTC+AGG - chr3.2:65972565-65972584 MS.gene29105:CDS 35.0%
! AGAAGAGCTTATTAGTCTCA+GGG - chr3.2:65972566-65972585 MS.gene29105:CDS 35.0%
! TTTTCGTTGCAGTACATTCA+TGG - chr3.2:65972468-65972487 MS.gene29105:intron 35.0%
AACTGGGAAAGAAACTCGAT+TGG + chr3.2:65972289-65972308 None:intergenic 40.0%
AGTACATTCATGGAGTTGGA+AGG - chr3.2:65972478-65972497 MS.gene29105:CDS 40.0%
CAATGTCCTGCAGTTATTCT+TGG + chr3.2:65972820-65972839 None:intergenic 40.0%
CAGAGGAATAAGGCATATTC+AGG - chr3.2:65972770-65972789 MS.gene29105:CDS 40.0%
GAATAACTGCAGGACATTGA+AGG - chr3.2:65972821-65972840 MS.gene29105:CDS 40.0%
TCACTCTCAATCATCTCCAA+AGG - chr3.2:65972249-65972268 MS.gene29105:CDS 40.0%
TCTCAACACTCCAAAGTCAA+AGG - chr3.2:65972308-65972327 MS.gene29105:CDS 40.0%
TTGCAGTACATTCATGGAGT+TGG - chr3.2:65972474-65972493 MS.gene29105:CDS 40.0%
TTGGAGATGATTGAGAGTGA+AGG + chr3.2:65972249-65972268 None:intergenic 40.0%
!! ACTTTGGAGTGTTGAGAACT+GGG + chr3.2:65972305-65972324 None:intergenic 40.0%
AACATTGTGTGCGTTCGTGT+TGG - chr3.2:65972411-65972430 MS.gene29105:intron 45.0%
ACAAACACTCTCAATGCCTG+TGG - chr3.2:65972221-65972240 MS.gene29105:CDS 45.0%
AGCGTACCAAGAATAACTGC+AGG - chr3.2:65972811-65972830 MS.gene29105:CDS 45.0%
ATTCAGGGACTTCCTTGTAG+AGG - chr3.2:65972786-65972805 MS.gene29105:CDS 45.0%
CAGGACATTGAAGGGTAAGA+AGG - chr3.2:65972830-65972849 MS.gene29105:CDS 45.0%
GGTAAGAAGGTTACTGTTGC+TGG - chr3.2:65972843-65972862 MS.gene29105:CDS 45.0%
TCAATCATCTCCAAAGGCCA+TGG - chr3.2:65972255-65972274 MS.gene29105:CDS 45.0%
!! GACTTTGGAGTGTTGAGAAC+TGG + chr3.2:65972306-65972325 None:intergenic 45.0%
ATTGTGTGCGTTCGTGTTGG+TGG - chr3.2:65972414-65972433 MS.gene29105:intron 50.0%
CTCGATTGGAGAAGTGACCA+TGG + chr3.2:65972275-65972294 None:intergenic 50.0%
TTGGTACGCTGTCCTCTACA+AGG + chr3.2:65972801-65972820 None:intergenic 50.0%
!! ATTGAGAGTGAAGGTGCCAC+AGG + chr3.2:65972240-65972259 None:intergenic 50.0%
GGAGAAGTGACCATGGCCTT+TGG + chr3.2:65972268-65972287 None:intergenic 55.0%
!! GTGCGTTCGTGTTGGTGGTG+TGG - chr3.2:65972419-65972438 MS.gene29105:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 65972216 65972881 65972216 ID=MS.gene29105
chr3.2 mRNA 65972216 65972881 65972216 ID=MS.gene29105.t1;Parent=MS.gene29105
chr3.2 exon 65972768 65972881 65972768 ID=MS.gene29105.t1.exon1;Parent=MS.gene29105.t1
chr3.2 CDS 65972768 65972881 65972768 ID=cds.MS.gene29105.t1;Parent=MS.gene29105.t1
chr3.2 exon 65972474 65972698 65972474 ID=MS.gene29105.t1.exon2;Parent=MS.gene29105.t1
chr3.2 CDS 65972474 65972698 65972474 ID=cds.MS.gene29105.t1;Parent=MS.gene29105.t1
chr3.2 exon 65972216 65972377 65972216 ID=MS.gene29105.t1.exon3;Parent=MS.gene29105.t1
chr3.2 CDS 65972216 65972377 65972216 ID=cds.MS.gene29105.t1;Parent=MS.gene29105.t1
Gene Sequence

>MS.gene29105

ATGGTACAAACACTCTCAATGCCTGTGGCACCTTCACTCTCAATCATCTCCAAAGGCCATGGTCACTTCTCCAATCGAGTTTCTTTCCCAGTTCTCAACACTCCAAAGTCAAAGGTAACACAATTAGGGTTTCAAAATTCAAGATAAATTCCAAATTCCATTCTGATTCTGATTGAATTGTAGGTTCAGAGGCTAAACATTGTGTGCGTTCGTGTTGGTGGTGTGGAAATTCCAAACAACAAGAGAATTGAATTTTCGTTGCAGTACATTCATGGAGTTGGAAGGAACAGAGCTAGACAGATTCTAACTGATATCAGTATGGAGAATAAAGTTACAAAGGAATTGAGTGAAGAAGAGCTTATTAGTCTCAGGGATGAAGTTAACAAATACATGACTGAAGGAGATTTGGTAATAAAAAAAATTAAAAATCTCATTTTTTTTGTTTTTTGTTTTCTATTATTTTGTTTTGTAATTGTAATGGTTTGATTTGTTTGTGAATTTCAGAGAAGGTTTAATGCACTTAATATAAAGAGGTTGAAAGAAATTCAGTGTTACAGAGGAATAAGGCATATTCAGGGACTTCCTTGTAGAGGACAGCGTACCAAGAATAACTGCAGGACATTGAAGGGTAAGAAGGTTACTGTTGCTGGAAAGAAGAAAAAGTAA

Protein sequence

>MS.gene29105.t1

MVQTLSMPVAPSLSIISKGHGHFSNRVSFPVLNTPKSKVQRLNIVCVRVGGVEIPNNKRIEFSLQYIHGVGRNRARQILTDISMENKVTKELSEEELISLRDEVNKYMTEGDLRRFNALNIKRLKEIQCYRGIRHIQGLPCRGQRTKNNCRTLKGKKVTVAGKKKK