AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36952


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36952.t1 MTR_8g013990 95.385 195 8 1 1 195 1 194 6.80e-137 380
MS.gene36952.t1 MTR_2g101140 66.332 199 58 5 3 195 1 196 2.37e-84 248
MS.gene36952.t1 MTR_8g021190 46.212 132 58 5 61 185 29 154 4.28e-31 111
MS.gene36952.t1 MTR_1g103550 47.778 90 39 3 62 148 2 86 3.48e-23 90.1
MS.gene36952.t1 MTR_3g105340 59.091 66 26 1 59 123 8 73 3.74e-22 87.4
MS.gene36952.t1 MTR_5g006430 43.750 96 45 3 62 154 2 91 2.34e-21 84.7
MS.gene36952.t1 MTR_3g109860 34.211 152 87 4 31 179 4 145 6.13e-19 79.7
MS.gene36952.t1 MTR_4g125030 47.222 72 37 1 53 123 13 84 1.43e-17 75.1
MS.gene36952.t1 MTR_4g125010 37.234 94 52 2 62 148 24 117 1.53e-17 76.3
MS.gene36952.t1 MTR_1g014930 40.217 92 47 2 47 134 1 88 9.84e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36952.t1 AT5G25840 50.543 184 54 5 35 195 4 173 2.52e-53 168
MS.gene36952.t1 AT1G79770 52.903 155 59 3 35 189 27 167 1.26e-46 151
MS.gene36952.t1 AT4G14819 42.727 110 51 3 62 171 2 99 1.70e-23 90.1
MS.gene36952.t1 AT2G25780 36.774 155 83 4 35 184 7 151 2.23e-22 88.6
MS.gene36952.t1 AT3G22540 40.449 89 50 1 62 150 2 87 2.19e-21 84.7
MS.gene36952.t1 AT1G72510 39.706 136 67 7 62 185 33 165 1.19e-20 84.7
MS.gene36952.t1 AT1G72510 39.706 136 67 7 62 185 33 165 1.19e-20 84.7
MS.gene36952.t1 AT1G54095 39.706 136 69 5 61 185 14 147 3.25e-19 80.5
MS.gene36952.t1 AT5G20670 33.582 134 82 3 59 185 19 152 4.36e-19 80.1
MS.gene36952.t1 AT2G09970 34.058 138 73 7 62 185 33 166 3.97e-16 72.8

Find 42 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACATGGATGGGAAAATT+AGG 0.194429 8.4:-76963281 MS.gene36952:CDS
CGACATTTCCTTCGAGTTGA+TGG 0.257929 8.4:-76963613 MS.gene36952:CDS
CCTCCTCCTTTCTTGTCCCT+TGG 0.275546 8.4:+76963243 None:intergenic
AAAATAGATAGATAGTTAAT+TGG 0.284549 8.4:+76963152 None:intergenic
TGTGTTAAGTTTAACAAATA+TGG 0.300385 8.4:-76963360 MS.gene36952:CDS
TATATTTCTTATCATGGTTA+TGG 0.301068 8.4:-76963745 None:intergenic
GCTTGTGCTTGAAACAAAAC+TGG 0.379794 8.4:+76963327 None:intergenic
GACAAGAAAGGAGGAGGAGG+AGG 0.400333 8.4:-76963237 MS.gene36952:CDS
GAAAGTTTCGTTGGTTTGTT+TGG 0.408422 8.4:+76963666 None:intergenic
TAACATGGATGGGAAAATTA+GGG 0.434099 8.4:-76963280 MS.gene36952:CDS
TTGATCTCTCTTGTAAGTGC+TGG 0.461637 8.4:+76963189 None:intergenic
ATTTGTGTGCGGTTTGTGTT+CGG 0.464682 8.4:-76963457 MS.gene36952:CDS
ACACTTTGCATCTTCAACTT+CGG 0.466067 8.4:+76963551 None:intergenic
TGTCGGAGACCGTTCTTTGA+AGG 0.474473 8.4:+76963631 None:intergenic
TTAAGTTTAACAAATATGGT+AGG 0.490124 8.4:-76963356 MS.gene36952:CDS
AGGGACAAGAAAGGAGGAGG+AGG 0.494514 8.4:-76963240 MS.gene36952:CDS
TGTTGTCAAGTTCATTCTCA+AGG 0.519029 8.4:-76963704 MS.gene36952:CDS
TTCTTTCGCCATCAACTCGA+AGG 0.530628 8.4:+76963605 None:intergenic
TAAGTTTAACAAATATGGTA+GGG 0.538103 8.4:-76963355 MS.gene36952:CDS
TCGGAAGTTGAAAGTTTCGT+TGG 0.539469 8.4:+76963657 None:intergenic
GGAGGCGGTGAAAGAAGAGT+TGG 0.546775 8.4:-76963436 MS.gene36952:CDS
GTGCGGTTTGTGTTCGGAGG+CGG 0.552330 8.4:-76963451 MS.gene36952:CDS
GACCGTTCTTTGAAGGTGGT+CGG 0.559329 8.4:+76963638 None:intergenic
ATCATGGTTATGGCACCTCA+TGG 0.562684 8.4:-76963735 MS.gene36952:CDS
CCAAGGGACAAGAAAGGAGG+AGG 0.566147 8.4:-76963243 MS.gene36952:CDS
CGGAGACCGTTCTTTGAAGG+TGG 0.567308 8.4:+76963634 None:intergenic
GGGCTAAGTCAATTAGTCCA+AGG 0.570684 8.4:-76963260 MS.gene36952:CDS
ATTAGTCCAAGGGACAAGAA+AGG 0.576487 8.4:-76963249 MS.gene36952:CDS
ACGAACACGATCTATGTACT+CGG 0.578833 8.4:+76963497 None:intergenic
CGTGTTCGTAACAAGTTCGA+AGG 0.581435 8.4:-76963483 MS.gene36952:CDS
ACAAGAAAGGAGGAGGAGGA+GGG 0.591395 8.4:-76963236 MS.gene36952:CDS
GGCTAAGTCAATTAGTCCAA+GGG 0.598516 8.4:-76963259 MS.gene36952:CDS
TTCCGACCACCTTCAAAGAA+CGG 0.607623 8.4:-76963640 MS.gene36952:CDS
CATCAACTCGAAGGAAATGT+CGG 0.632792 8.4:+76963614 None:intergenic
TCTTCAACTTCGGATATCGA+TGG 0.633325 8.4:+76963561 None:intergenic
TGTGTGCGGTTTGTGTTCGG+AGG 0.638466 8.4:-76963454 MS.gene36952:CDS
GTGTTCGTAACAAGTTCGAA+GGG 0.639090 8.4:-76963482 MS.gene36952:CDS
TTCGAAGGGAAATTTGTGTG+CGG 0.644656 8.4:-76963468 MS.gene36952:CDS
CGAACACGATCTATGTACTC+GGG 0.649900 8.4:+76963498 None:intergenic
GATGCAAAGTGTGAGTGTTG+TGG 0.665594 8.4:-76963540 MS.gene36952:CDS
AGTCCAAGGGACAAGAAAGG+AGG 0.666513 8.4:-76963246 MS.gene36952:CDS
GACAACAAACATTCTCCATG+AGG 0.716500 8.4:+76963720 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGTTTAACAAATATGGTA+GGG - chr8.4:76963544-76963563 MS.gene36952:CDS 20.0%
!! TGTGTTAAGTTTAACAAATA+TGG - chr8.4:76963539-76963558 MS.gene36952:CDS 20.0%
!! TTAAGTTTAACAAATATGGT+AGG - chr8.4:76963543-76963562 MS.gene36952:CDS 20.0%
! AATGTTGAAAAAGAGTAACA+TGG - chr8.4:76963604-76963623 MS.gene36952:CDS 25.0%
AAAGAAGAGTTGGAGAAAAA+TGG - chr8.4:76963473-76963492 MS.gene36952:CDS 30.0%
TAACATGGATGGGAAAATTA+GGG - chr8.4:76963619-76963638 MS.gene36952:CDS 30.0%
TGAAAAAGAGTAACATGGAT+GGG - chr8.4:76963609-76963628 MS.gene36952:CDS 30.0%
TTGAAAAAGAGTAACATGGA+TGG - chr8.4:76963608-76963627 MS.gene36952:CDS 30.0%
AAGAGTTGGAGAAAAATGGT+GGG - chr8.4:76963477-76963496 MS.gene36952:CDS 35.0%
ACACTTTGCATCTTCAACTT+CGG + chr8.4:76963351-76963370 None:intergenic 35.0%
GTAACATGGATGGGAAAATT+AGG - chr8.4:76963618-76963637 MS.gene36952:CDS 35.0%
TGTTGTCAAGTTCATTCTCA+AGG - chr8.4:76963195-76963214 MS.gene36952:CDS 35.0%
!! GAAAGTTTCGTTGGTTTGTT+TGG + chr8.4:76963236-76963255 None:intergenic 35.0%
ACGAACACGATCTATGTACT+CGG + chr8.4:76963405-76963424 None:intergenic 40.0%
ATTAGTCCAAGGGACAAGAA+AGG - chr8.4:76963650-76963669 MS.gene36952:CDS 40.0%
ATTTGTGTGCGGTTTGTGTT+CGG - chr8.4:76963442-76963461 MS.gene36952:CDS 40.0%
CATCAACTCGAAGGAAATGT+CGG + chr8.4:76963288-76963307 None:intergenic 40.0%
GAAGAGTTGGAGAAAAATGG+TGG - chr8.4:76963476-76963495 MS.gene36952:CDS 40.0%
GACAACAAACATTCTCCATG+AGG + chr8.4:76963182-76963201 None:intergenic 40.0%
GCTTGTGCTTGAAACAAAAC+TGG + chr8.4:76963575-76963594 None:intergenic 40.0%
GGCTAAGTCAATTAGTCCAA+GGG - chr8.4:76963640-76963659 MS.gene36952:CDS 40.0%
GTGTTCGTAACAAGTTCGAA+GGG - chr8.4:76963417-76963436 MS.gene36952:CDS 40.0%
TCGGAAGTTGAAAGTTTCGT+TGG + chr8.4:76963245-76963264 None:intergenic 40.0%
TCTTCAACTTCGGATATCGA+TGG + chr8.4:76963341-76963360 None:intergenic 40.0%
TTCGAAGGGAAATTTGTGTG+CGG - chr8.4:76963431-76963450 MS.gene36952:CDS 40.0%
TTGATCTCTCTTGTAAGTGC+TGG + chr8.4:76963713-76963732 None:intergenic 40.0%
CGAACACGATCTATGTACTC+GGG + chr8.4:76963404-76963423 None:intergenic 45.0%
CGTGTTCGTAACAAGTTCGA+AGG - chr8.4:76963416-76963435 MS.gene36952:CDS 45.0%
GGGCTAAGTCAATTAGTCCA+AGG - chr8.4:76963639-76963658 MS.gene36952:CDS 45.0%
TTCCGACCACCTTCAAAGAA+CGG - chr8.4:76963259-76963278 MS.gene36952:CDS 45.0%
TTCTTTCGCCATCAACTCGA+AGG + chr8.4:76963297-76963316 None:intergenic 45.0%
! GATGCAAAGTGTGAGTGTTG+TGG - chr8.4:76963359-76963378 MS.gene36952:CDS 45.0%
!! CGACATTTCCTTCGAGTTGA+TGG - chr8.4:76963286-76963305 MS.gene36952:CDS 45.0%
ACAAGAAAGGAGGAGGAGGA+GGG - chr8.4:76963663-76963682 MS.gene36952:CDS 50.0%
AGTCCAAGGGACAAGAAAGG+AGG - chr8.4:76963653-76963672 MS.gene36952:CDS 50.0%
TGTCGGAGACCGTTCTTTGA+AGG + chr8.4:76963271-76963290 None:intergenic 50.0%
! GACCGTTCTTTGAAGGTGGT+CGG + chr8.4:76963264-76963283 None:intergenic 50.0%
AGGGACAAGAAAGGAGGAGG+AGG - chr8.4:76963659-76963678 MS.gene36952:CDS 55.0%
CCAAGGGACAAGAAAGGAGG+AGG - chr8.4:76963656-76963675 MS.gene36952:CDS 55.0%
CCTCCTCCTTTCTTGTCCCT+TGG + chr8.4:76963659-76963678 None:intergenic 55.0%
GACAAGAAAGGAGGAGGAGG+AGG - chr8.4:76963662-76963681 MS.gene36952:CDS 55.0%
GGAGGCGGTGAAAGAAGAGT+TGG - chr8.4:76963463-76963482 MS.gene36952:CDS 55.0%
TGTGTGCGGTTTGTGTTCGG+AGG - chr8.4:76963445-76963464 MS.gene36952:CDS 55.0%
! CGGAGACCGTTCTTTGAAGG+TGG + chr8.4:76963268-76963287 None:intergenic 55.0%
GTGCGGTTTGTGTTCGGAGG+CGG - chr8.4:76963448-76963467 MS.gene36952:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 76963167 76963754 76963167 ID=MS.gene36952
chr8.4 mRNA 76963167 76963754 76963167 ID=MS.gene36952.t1;Parent=MS.gene36952
chr8.4 exon 76963167 76963754 76963167 ID=MS.gene36952.t1.exon1;Parent=MS.gene36952.t1
chr8.4 CDS 76963167 76963754 76963167 ID=cds.MS.gene36952.t1;Parent=MS.gene36952.t1
Gene Sequence

>MS.gene36952

ATGGTTATGGCACCTCATGGAGAATGTTTGTTGTCAAGTTCATTCTCAAGGAACAACAACAACACACCAAACAAACCAACGAAACTTTCAACTTCCGACCACCTTCAAAGAACGGTCTCCGACATTTCCTTCGAGTTGATGGCGAAAGAAGCAATCGATAATTTAAAACTACCATCGATATCCGAAGTTGAAGATGCAAAGTGTGAGTGTTGTGGCATGTCTGAAGAGTGCACACCCGAGTACATAGATCGTGTTCGTAACAAGTTCGAAGGGAAATTTGTGTGCGGTTTGTGTTCGGAGGCGGTGAAAGAAGAGTTGGAGAAAAATGGTGGGAAAAAATTAGAAGAAGCTTTAAACACACATATGAATGCATGTGTTAAGTTTAACAAATATGGTAGGGCTTTTCCAGTTTTGTTTCAAGCACAAGCTATGAAAGAAATGTTGAAAAAGAGTAACATGGATGGGAAAATTAGGGCTAAGTCAATTAGTCCAAGGGACAAGAAAGGAGGAGGAGGAGGGCTTGCTCGTAGTACAAGTTGTATTCCAGCACTTACAAGAGAGATCAACAATATCAAAATAGCCAATTAA

Protein sequence

>MS.gene36952.t1

MVMAPHGECLLSSSFSRNNNNTPNKPTKLSTSDHLQRTVSDISFELMAKEAIDNLKLPSISEVEDAKCECCGMSEECTPEYIDRVRNKFEGKFVCGLCSEAVKEELEKNGGKKLEEALNTHMNACVKFNKYGRAFPVLFQAQAMKEMLKKSNMDGKIRAKSISPRDKKGGGGGLARSTSCIPALTREINNIKIAN