AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069859


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069859.t1 MTR_2g101380 96.335 191 7 0 1 191 1 191 3.46e-137 381
MS.gene069859.t1 MTR_1g011510 53.846 104 46 2 72 174 60 162 1.99e-24 94.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069859.t1 AT3G16250 64.088 181 52 5 18 186 20 199 3.67e-77 229
MS.gene069859.t1 AT4G32590 53.846 104 46 2 72 174 70 172 1.80e-25 97.1
MS.gene069859.t1 AT4G32590 49.074 108 53 2 57 163 55 161 3.55e-19 80.9

Find 43 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGCTAATCTCCAATTCTT+TGG 0.246574 2.1:-3778846 None:intergenic
AGTGCCGACTGCTCTAATTT+TGG 0.281013 2.1:-3778028 None:intergenic
GAGAGACGTGTGAATGCATT+AGG 0.321958 2.1:-3777955 None:intergenic
GACAAATGCAAAACCTATAG+AGG 0.334673 2.1:-3778100 None:intergenic
CTTTATAGAAACCAAAGAAT+TGG 0.359961 2.1:+3778835 MS.gene069859:intron
GATTCTAACATTGAACTCTA+TGG 0.373731 2.1:+3778351 MS.gene069859:CDS
TAGGGGTTAGTTTGAGTTGG+AGG 0.393377 2.1:-3777936 None:intergenic
TCTAATGACCCTCCCTCTAT+AGG 0.394740 2.1:+3778087 MS.gene069859:CDS
CTTGTAAAGAGTGTTGTGTT+TGG 0.421734 2.1:-3778003 None:intergenic
CATTCATGTCCTTTCCACTC+TGG 0.430702 2.1:-3779052 None:intergenic
GAACACCAGATGTGCATTAT+CGG 0.433345 2.1:+3778289 MS.gene069859:CDS
AGAGACGTGTGAATGCATTA+GGG 0.437849 2.1:-3777954 None:intergenic
TGCACATCTGGTGTTCCATC+TGG 0.437999 2.1:-3778282 None:intergenic
CATTAGGGGTTAGTTTGAGT+TGG 0.450485 2.1:-3777939 None:intergenic
ATTTGCTATTAGGTTCTTGA+AGG 0.451453 2.1:+3778610 MS.gene069859:intron
GCTGTCCGATAATGCACATC+TGG 0.465486 2.1:-3778294 None:intergenic
GTGGGTGAAGCAGATTCTAC+AGG 0.486617 2.1:+3778879 MS.gene069859:CDS
CTATTAGGTTCTTGAAGGCA+AGG 0.503895 2.1:+3778615 MS.gene069859:intron
GAAGCTTAGGAACATAATGT+TGG 0.511451 2.1:+3778329 MS.gene069859:CDS
CTTGTGGTGGACAGAAGCTT+AGG 0.512735 2.1:+3778316 MS.gene069859:CDS
ACATGAATGGAAATATGAAA+AGG 0.521149 2.1:+3779066 MS.gene069859:CDS
CAGAGTGGAAAGGACATGAA+TGG 0.529406 2.1:+3779053 MS.gene069859:CDS
TGGAACTTGTGCTACATGTA+TGG 0.561485 2.1:+3778489 MS.gene069859:CDS
TAGTCTGTGTTGCTACCAGA+TGG 0.569681 2.1:+3778267 MS.gene069859:intron
TGGGTGAAGCAGATTCTACA+GGG 0.575014 2.1:+3778880 MS.gene069859:CDS
CGGCACTGTCCCTGAAAAGA+CGG 0.578806 2.1:+3778044 MS.gene069859:CDS
TCTTTCTCTTTGTCAGTGCG+AGG 0.589508 2.1:-3778649 None:intergenic
TTAGCATGTCAAACAACTGT+GGG 0.604116 2.1:+3778861 MS.gene069859:CDS
TTGTGCTACATGTATGGTAG+AGG 0.614537 2.1:+3778495 MS.gene069859:CDS
AGAACCAAAATTAGAGCAGT+CGG 0.615630 2.1:+3778024 MS.gene069859:CDS
ACAAATGCAAAACCTATAGA+GGG 0.622761 2.1:-3778099 None:intergenic
GACTCAGCAGACTCTTTGTG+AGG 0.642468 2.1:+3779097 MS.gene069859:CDS
CAACAATTACCAGAGTGGAA+AGG 0.648231 2.1:+3779043 MS.gene069859:CDS
GTGCATTATCGGACAGCTTG+TGG 0.648882 2.1:+3778300 MS.gene069859:CDS
TGAAGCAGATTCTACAGGGG+TGG 0.651914 2.1:+3778884 MS.gene069859:CDS
ATGCAAAACCTATAGAGGGA+GGG 0.653145 2.1:-3778095 None:intergenic
GAGACGTGTGAATGCATTAG+GGG 0.653996 2.1:-3777953 None:intergenic
AATGCAAAACCTATAGAGGG+AGG 0.655010 2.1:-3778096 None:intergenic
ATTAGCATGTCAAACAACTG+TGG 0.658456 2.1:+3778860 MS.gene069859:CDS
TGATTCAACAATTACCAGAG+TGG 0.670303 2.1:+3779038 MS.gene069859:CDS
CATTATCGGACAGCTTGTGG+TGG 0.674346 2.1:+3778303 MS.gene069859:CDS
AGGGTCATTAGAGTCACTAG+TGG 0.691443 2.1:-3778076 None:intergenic
GGGTGAAGCAGATTCTACAG+GGG 0.770570 2.1:+3778881 MS.gene069859:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTTAAGCAATAAT+TGG + chr2.1:3778406-3778425 MS.gene069859:intron 10.0%
!!! TTTTTTTTTAATTTGCTATT+AGG + chr2.1:3778600-3778619 MS.gene069859:intron 10.0%
!! CAGATTAGAAATTAATAAAA+TGG + chr2.1:3778941-3778960 MS.gene069859:intron 15.0%
!! ATTTATGTTGAGCAATATAT+TGG + chr2.1:3778986-3779005 MS.gene069859:intron 20.0%
!! TAATTCTACTTATATCTAGT+TGG + chr2.1:3778193-3778212 MS.gene069859:intron 20.0%
!!! AATTAATCTCTAATGTTTTG+TGG + chr2.1:3778242-3778261 MS.gene069859:intron 20.0%
!!! AGCAAAAATACACATTTTAT+TGG + chr2.1:3778547-3778566 MS.gene069859:intron 20.0%
!!! GCAAAAATACACATTTTATT+GGG + chr2.1:3778548-3778567 MS.gene069859:intron 20.0%
!!! GTTAGTACTACTAATTTTTT+GGG + chr2.1:3778906-3778925 MS.gene069859:intron 20.0%
! AAGAGAAAGAAAAACTTAAG+AGG + chr2.1:3778662-3778681 MS.gene069859:CDS 25.0%
! AATTTAACAAAATTCTACCC+TGG - chr2.1:3778459-3778478 None:intergenic 25.0%
! ACATGAATGGAAATATGAAA+AGG + chr2.1:3779066-3779085 MS.gene069859:CDS 25.0%
! AGAGAAAGAAAAACTTAAGA+GGG + chr2.1:3778663-3778682 MS.gene069859:CDS 25.0%
! CTTTATAGAAACCAAAGAAT+TGG + chr2.1:3778835-3778854 MS.gene069859:intron 25.0%
! GTTAGTGAGATAATTACATA+AGG - chr2.1:3778378-3778397 None:intergenic 25.0%
!! AATTTTGTTAAATTGTGGAG+GGG + chr2.1:3778465-3778484 MS.gene069859:CDS 25.0%
!! AGAATTTTGTTAAATTGTGG+AGG + chr2.1:3778463-3778482 MS.gene069859:CDS 25.0%
!! GAATTTTGTTAAATTGTGGA+GGG + chr2.1:3778464-3778483 MS.gene069859:CDS 25.0%
!! GGTAGAATTTTGTTAAATTG+TGG + chr2.1:3778460-3778479 MS.gene069859:CDS 25.0%
!!! ATTTTGTTGTTGATTTTTCC+AGG + chr2.1:3778438-3778457 MS.gene069859:intron 25.0%
!!! GGTTAGTACTACTAATTTTT+TGG + chr2.1:3778905-3778924 MS.gene069859:intron 25.0%
!!! TTTTGTTGTTGATTTTTCCA+GGG + chr2.1:3778439-3778458 MS.gene069859:intron 25.0%
ACAAATGCAAAACCTATAGA+GGG - chr2.1:3778102-3778121 None:intergenic 30.0%
GATTCTAACATTGAACTCTA+TGG + chr2.1:3778351-3778370 MS.gene069859:CDS 30.0%
! ATTTTCATTCCATGTGATGT+AGG + chr2.1:3779012-3779031 MS.gene069859:intron 30.0%
! ATTTTGTTAAATTGTGGAGG+GGG + chr2.1:3778466-3778485 MS.gene069859:CDS 30.0%
!! ATTTGCTATTAGGTTCTTGA+AGG + chr2.1:3778610-3778629 MS.gene069859:intron 30.0%
!!! TGGTCTGAATTTTTTATGTG+AGG - chr2.1:3777986-3778005 None:intergenic 30.0%
AGAACCAAAATTAGAGCAGT+CGG + chr2.1:3778024-3778043 MS.gene069859:CDS 35.0%
ATGTGAAATGTGAACTCTTG+TGG + chr2.1:3778778-3778797 MS.gene069859:intron 35.0%
ATTAGCATGTCAAACAACTG+TGG + chr2.1:3778860-3778879 MS.gene069859:CDS 35.0%
CATGCTAATCTCCAATTCTT+TGG - chr2.1:3778849-3778868 None:intergenic 35.0%
GAAGCTTAGGAACATAATGT+TGG + chr2.1:3778329-3778348 MS.gene069859:CDS 35.0%
GACAAATGCAAAACCTATAG+AGG - chr2.1:3778103-3778122 None:intergenic 35.0%
TGATTCAACAATTACCAGAG+TGG + chr2.1:3779038-3779057 MS.gene069859:CDS 35.0%
TTAGCATGTCAAACAACTGT+GGG + chr2.1:3778861-3778880 MS.gene069859:CDS 35.0%
!! CTTGTAAAGAGTGTTGTGTT+TGG - chr2.1:3778006-3778025 None:intergenic 35.0%
AATGCAAAACCTATAGAGGG+AGG - chr2.1:3778099-3778118 None:intergenic 40.0%
AGAGACGTGTGAATGCATTA+GGG - chr2.1:3777957-3777976 None:intergenic 40.0%
ATGCAAAACCTATAGAGGGA+GGG - chr2.1:3778098-3778117 None:intergenic 40.0%
CAACAATTACCAGAGTGGAA+AGG + chr2.1:3779043-3779062 MS.gene069859:CDS 40.0%
CTATTAGGTTCTTGAAGGCA+AGG + chr2.1:3778615-3778634 MS.gene069859:intron 40.0%
GAACACCAGATGTGCATTAT+CGG + chr2.1:3778289-3778308 MS.gene069859:CDS 40.0%
GAATCACAACCTACATCACA+TGG - chr2.1:3779024-3779043 None:intergenic 40.0%
TGGAACTTGTGCTACATGTA+TGG + chr2.1:3778489-3778508 MS.gene069859:CDS 40.0%
TTGTGCTACATGTATGGTAG+AGG + chr2.1:3778495-3778514 MS.gene069859:CDS 40.0%
! TCTCTGAATCCGTCTTTTCA+GGG - chr2.1:3778056-3778075 None:intergenic 40.0%
!! CATTAGGGGTTAGTTTGAGT+TGG - chr2.1:3777942-3777961 None:intergenic 40.0%
AGGGTCATTAGAGTCACTAG+TGG - chr2.1:3778079-3778098 None:intergenic 45.0%
CAGAGTGGAAAGGACATGAA+TGG + chr2.1:3779053-3779072 MS.gene069859:CDS 45.0%
CATTCATGTCCTTTCCACTC+TGG - chr2.1:3779055-3779074 None:intergenic 45.0%
GAGACGTGTGAATGCATTAG+GGG - chr2.1:3777956-3777975 None:intergenic 45.0%
GAGAGACGTGTGAATGCATT+AGG - chr2.1:3777958-3777977 None:intergenic 45.0%
TCTAATGACCCTCCCTCTAT+AGG + chr2.1:3778087-3778106 MS.gene069859:CDS 45.0%
TCTTTCTCTTTGTCAGTGCG+AGG - chr2.1:3778652-3778671 None:intergenic 45.0%
TGGGTGAAGCAGATTCTACA+GGG + chr2.1:3778880-3778899 MS.gene069859:CDS 45.0%
TTGTTAAATTGTGGAGGGGG+TGG + chr2.1:3778469-3778488 MS.gene069859:CDS 45.0%
! GTCTCTGAATCCGTCTTTTC+AGG - chr2.1:3778057-3778076 None:intergenic 45.0%
! TAGTCTGTGTTGCTACCAGA+TGG + chr2.1:3778267-3778286 MS.gene069859:intron 45.0%
!! TAGGGGTTAGTTTGAGTTGG+AGG - chr2.1:3777939-3777958 None:intergenic 45.0%
!!! AGTGCCGACTGCTCTAATTT+TGG - chr2.1:3778031-3778050 None:intergenic 45.0%
CATTATCGGACAGCTTGTGG+TGG + chr2.1:3778303-3778322 MS.gene069859:CDS 50.0%
GCTGTCCGATAATGCACATC+TGG - chr2.1:3778297-3778316 None:intergenic 50.0%
GGGTGAAGCAGATTCTACAG+GGG + chr2.1:3778881-3778900 MS.gene069859:CDS 50.0%
GTGCATTATCGGACAGCTTG+TGG + chr2.1:3778300-3778319 MS.gene069859:CDS 50.0%
GTGGGTGAAGCAGATTCTAC+AGG + chr2.1:3778879-3778898 MS.gene069859:CDS 50.0%
TGAAGCAGATTCTACAGGGG+TGG + chr2.1:3778884-3778903 MS.gene069859:CDS 50.0%
TGCACATCTGGTGTTCCATC+TGG - chr2.1:3778285-3778304 None:intergenic 50.0%
!! CTTGTGGTGGACAGAAGCTT+AGG + chr2.1:3778316-3778335 MS.gene069859:CDS 50.0%
CGGCACTGTCCCTGAAAAGA+CGG + chr2.1:3778044-3778063 MS.gene069859:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 3777928 3779117 3777928 ID=MS.gene069859
chr2.1 mRNA 3777928 3779117 3777928 ID=MS.gene069859.t1;Parent=MS.gene069859
chr2.1 exon 3777928 3778125 3777928 ID=MS.gene069859.t1.exon1;Parent=MS.gene069859.t1
chr2.1 CDS 3777928 3778125 3777928 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
chr2.1 exon 3778270 3778380 3778270 ID=MS.gene069859.t1.exon2;Parent=MS.gene069859.t1
chr2.1 CDS 3778270 3778380 3778270 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
chr2.1 exon 3778460 3778516 3778460 ID=MS.gene069859.t1.exon3;Parent=MS.gene069859.t1
chr2.1 CDS 3778460 3778516 3778460 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
chr2.1 exon 3778622 3778684 3778622 ID=MS.gene069859.t1.exon4;Parent=MS.gene069859.t1
chr2.1 CDS 3778622 3778684 3778622 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
chr2.1 exon 3778843 3778905 3778843 ID=MS.gene069859.t1.exon5;Parent=MS.gene069859.t1
chr2.1 CDS 3778843 3778905 3778843 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
chr2.1 exon 3779034 3779117 3779034 ID=MS.gene069859.t1.exon6;Parent=MS.gene069859.t1
chr2.1 CDS 3779034 3779117 3779034 ID=cds.MS.gene069859.t1;Parent=MS.gene069859.t1
Gene Sequence

>MS.gene069859

ATGTCACTCCTCCAACTCAAACTAACCCCTAATGCATTCACACGTCTCTCTTCTTCCTCACATAAAAAATTCAGACCAAACACAACACTCTTTACAAGAACCAAAATTAGAGCAGTCGGCACTGTCCCTGAAAAGACGGATTCAGAGACCACTAGTGACTCTAATGACCCTCCCTCTATAGGTTTTGCATTTGTCAGTGTAAGTTCAAATTCATAATACTATACTAAATGTACATACATGTAACATAACATAACATGTAACTAATTAATTCTACTTATATCTAGTTGGTTTTACATTTATTATTAAATTTATGAAATTAATCTCTAATGTTTTGTGGATTAGTCTGTGTTGCTACCAGATGGAACACCAGATGTGCATTATCGGACAGCTTGTGGTGGACAGAAGCTTAGGAACATAATGTTGGATTCTAACATTGAACTCTATGGACCTTATGTAATTATCTCACTAACTTAAATTATTTTATTTTTAAGCAATAATTGGATGATAATTATTTTGTTGTTGATTTTTCCAGGGTAGAATTTTGTTAAATTGTGGAGGGGGTGGAACTTGTGCTACATGTATGGTAGAGGTGAGCTATAATAATCTTATATAATTTTCTAGCAAAAATACACATTTTATTGGGTAGTATATAGTTATATAGTACTTATTGTATTTTTTTTTAATTTGCTATTAGGTTCTTGAAGGCAAGGAGCTTCTGAGTCCTCGCACTGACAAAGAGAAAGAAAAACTTAAGAGGGTAAATGATTAAATCTTAACTTGATTAATGTTATTTTTTAATAAGAACTTAATTGTCATATTGAAAGAAAGAACAAACTTTTAGCATAAGTATATGTGAAATGTGAACTCTTGTGGTAAGAAAGATTACAATTATGATATCTTGTGTTTTCTTTATAGAAACCAAAGAATTGGAGATTAGCATGTCAAACAACTGTGGGTGAAGCAGATTCTACAGGGGTGGTTAGTACTACTAATTTTTTGGGTTAATTAATATACAGATTAGAAATTAATAAAATGGCTGTATTTATTATTTATGCTTAATTTATGTTGAGCAATATATTGGTACATTTTCATTCCATGTGATGTAGGTTGTGATTCAACAATTACCAGAGTGGAAAGGACATGAATGGAAATATGAAAAGGATGATGATGACTCAGCAGACTCTTTGTGA

Protein sequence

>MS.gene069859.t1

MSLLQLKLTPNAFTRLSSSSHKKFRPNTTLFTRTKIRAVGTVPEKTDSETTSDSNDPPSIGFAFVSSVLLPDGTPDVHYRTACGGQKLRNIMLDSNIELYGPYGRILLNCGGGGTCATCMVEVLEGKELLSPRTDKEKEKLKRKPKNWRLACQTTVGEADSTGVVVIQQLPEWKGHEWKYEKDDDDSADSL