AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069717.t1 MTR_8g046460 93.284 268 12 2 1 262 1 268 5.61e-178 490
MS.gene069717.t1 MTR_2g064370 70.522 268 72 4 1 262 1 267 3.79e-131 372
MS.gene069717.t1 MTR_8g028425 57.143 154 63 2 72 223 92 244 5.45e-65 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069717.t1 AT1G16320 69.231 221 67 1 43 262 53 273 4.49e-117 336
MS.gene069717.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene069717.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene069717.t1 AT2G46220 57.325 157 64 2 69 223 81 236 2.50e-66 206

Find 57 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAACCGGTTTCGGTTT+TGG 0.220818 8.4:+54306905 MS.gene069717:CDS
GACCCTTTGAACACATTTAC+TGG 0.240823 8.4:+54306621 MS.gene069717:CDS
AGAAGTACAAGTTGATCTTT+TGG 0.270946 8.4:+54306649 MS.gene069717:CDS
AGGTACTTCGCGGTATAAAT+TGG 0.272271 8.4:+54306818 MS.gene069717:CDS
ACCTTAATTATGATATTTAT+AGG 0.278702 8.4:+54306155 MS.gene069717:CDS
ACCTATAAATATCATAATTA+AGG 0.297853 8.4:-54306156 None:intergenic
CCCCATGAAAATGTTGGATT+TGG 0.313331 8.4:-54306954 None:intergenic
TGGATTCTAGTTCATGTTCA+TGG 0.337564 8.4:+54306982 MS.gene069717:CDS
GAATAAAGAGTATTGAGAAA+TGG 0.346750 8.4:-54305963 None:intergenic
AATAAAGAGTATTGAGAAAT+GGG 0.349742 8.4:-54305962 None:intergenic
GTTTGGATGTATACAGAATA+TGG 0.351747 8.4:+54306712 MS.gene069717:CDS
TTGGTGTTTGAATCTTGATT+TGG 0.370847 8.4:-54305921 None:intergenic
TGTTTATCTTGAGTAGCTAA+TGG 0.394872 8.4:-54306043 None:intergenic
GAAGATGATGAAACCGGTTT+CGG 0.396965 8.4:+54306899 MS.gene069717:CDS
GTTGTTACCACCAGATGGTT+TGG 0.415329 8.4:+54307052 MS.gene069717:CDS
GGAACTTGAGGGGTGTGCCT+AGG 0.417152 8.4:+54306766 MS.gene069717:CDS
TTTGTTTCGTGAGATTGGTT+TGG 0.421815 8.4:+54306695 MS.gene069717:CDS
GAACTTGAGGGGTGTGCCTA+GGG 0.426698 8.4:+54306767 MS.gene069717:CDS
ACCAAATCCAAAACCGAAAC+CGG 0.442141 8.4:-54306912 None:intergenic
CAACCTGTGCAGTGAAACTT+TGG 0.444081 8.4:-54306006 None:intergenic
AATGTTGGATTTGGACTAGC+AGG 0.451399 8.4:-54306945 None:intergenic
TTTCACTGCACAGGTTGCTC+AGG 0.452221 8.4:+54306012 MS.gene069717:CDS
GGGGTGTGCCTAGGGTTCCA+TGG 0.454206 8.4:+54306775 MS.gene069717:CDS
GGGTGTGCCTAGGGTTCCAT+GGG 0.462556 8.4:+54306776 MS.gene069717:CDS
TTGAATCTTGATTTGGATTG+AGG 0.469278 8.4:-54305914 None:intergenic
GAACATGCAGCCAAACCATC+TGG 0.478212 8.4:-54307062 None:intergenic
CAGAGAATGTGATATTGATC+AGG 0.478427 8.4:+54306742 MS.gene069717:CDS
TACCAGTAAATGTGTTCAAA+GGG 0.481927 8.4:-54306623 None:intergenic
AGTCCAAAGTTTCACTGCAC+AGG 0.485891 8.4:+54306003 MS.gene069717:CDS
TCCACAGAAGATGATGAAAC+CGG 0.487224 8.4:+54306893 MS.gene069717:CDS
ATACCAGTAAATGTGTTCAA+AGG 0.496720 8.4:-54306624 None:intergenic
TAAACAGAATCAGCATCAAA+AGG 0.500362 8.4:+54306060 MS.gene069717:CDS
GAAGCTAAAGGTGAATTTCA+AGG 0.517611 8.4:+54306798 MS.gene069717:CDS
GTGAATTTCAAGGTACTTCG+CGG 0.529074 8.4:+54306808 MS.gene069717:CDS
ATCAATATCACATTCTCTGA+AGG 0.533171 8.4:-54306738 None:intergenic
AATTCACCTTTAGCTTCCCA+TGG 0.534577 8.4:-54306792 None:intergenic
TTAGCTTCCCATGGAACCCT+AGG 0.536034 8.4:-54306783 None:intergenic
TATTGATCAGGTGGAACTTG+AGG 0.540071 8.4:+54306754 MS.gene069717:CDS
AAAGGACCCCATGAAAATGT+TGG 0.541364 8.4:-54306960 None:intergenic
ATTGATCAGGTGGAACTTGA+GGG 0.551874 8.4:+54306755 MS.gene069717:CDS
ACCGGTTTCATCATCTTCTG+TGG 0.561359 8.4:-54306894 None:intergenic
AGGGTTCCATGGGAAGCTAA+AGG 0.578430 8.4:+54306786 MS.gene069717:CDS
GAAAGATTGAGATTGCAAAG+AGG 0.578460 8.4:-54305884 None:intergenic
AGGTCTTTAACAAAGATCAA+AGG 0.587544 8.4:-54306136 None:intergenic
AGACAGTTGTTACCACCAGA+TGG 0.589181 8.4:+54307047 MS.gene069717:CDS
GCAGTGAAACTTTGGACTGA+AGG 0.593412 8.4:-54305998 None:intergenic
AGAATGTGATATTGATCAGG+TGG 0.610163 8.4:+54306745 MS.gene069717:CDS
TCAAGCAGTTAAGCACACAT+TGG 0.624208 8.4:+54307016 MS.gene069717:CDS
CTTCGCGGTATAAATTGGAT+CGG 0.643822 8.4:+54306823 MS.gene069717:CDS
GAACATGAACTAGAATCCAA+AGG 0.647700 8.4:-54306978 None:intergenic
TGCTGTTAGAACCCTTCGTG+AGG 0.651000 8.4:+54306108 MS.gene069717:CDS
CGGTATAAATTGGATCGGAA+CGG 0.651485 8.4:+54306828 MS.gene069717:CDS
TTGATCAGGTGGAACTTGAG+GGG 0.654298 8.4:+54306756 MS.gene069717:CDS
CAAAGGCATATCCTCACGAA+GGG 0.657035 8.4:-54306119 None:intergenic
ATGTTGGATTTGGACTAGCA+GGG 0.662829 8.4:-54306944 None:intergenic
TCAAAGGCATATCCTCACGA+AGG 0.664210 8.4:-54306120 None:intergenic
CATGCAGCCAAACCATCTGG+TGG 0.695452 8.4:-54307059 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTATAAATATCATAATTA+AGG - chr8.4:54306159-54306178 None:intergenic 15.0%
!! ACCTTAATTATGATATTTAT+AGG + chr8.4:54306155-54306174 MS.gene069717:CDS 15.0%
!! GATGATGTAATTAATATTTA+TGG + chr8.4:54306304-54306323 MS.gene069717:intron 15.0%
!!! AAAAATTGGATTTTTAATGT+TGG + chr8.4:54306265-54306284 MS.gene069717:intron 15.0%
!!! AAAATTGGATTTTTAATGTT+GGG + chr8.4:54306266-54306285 MS.gene069717:intron 15.0%
!!! ATAGTTAGAATTTTAATGTT+GGG + chr8.4:54306562-54306581 MS.gene069717:intron 15.0%
!!! TAAAAAAAGTTAATTTTGAG+TGG + chr8.4:54306507-54306526 MS.gene069717:intron 15.0%
!!! TATAGTTAGAATTTTAATGT+TGG + chr8.4:54306561-54306580 MS.gene069717:intron 15.0%
!!! TTGCTTTTCATATAAATATA+TGG + chr8.4:54306208-54306227 MS.gene069717:intron 15.0%
!!! AATAAAGAGTATTGAGAAAT+GGG - chr8.4:54305965-54305984 None:intergenic 20.0%
!!! AATTTTGAGTGGAAAAAAAT+TGG + chr8.4:54306518-54306537 MS.gene069717:intron 20.0%
!!! ATTTCTAGGTGAAAAAAAAT+TGG + chr8.4:54306251-54306270 MS.gene069717:intron 20.0%
!!! ATTTGAATTGTGTTATTTCT+AGG + chr8.4:54306237-54306256 MS.gene069717:intron 20.0%
!!! ATTTTGAGTGGAAAAAAATT+GGG + chr8.4:54306519-54306538 MS.gene069717:intron 20.0%
!!! CTTTTTTTATACATAGAAAG+TGG - chr8.4:54306496-54306515 None:intergenic 20.0%
! ATTAATATTTATGGACCCTT+TGG + chr8.4:54306313-54306332 MS.gene069717:intron 25.0%
! TTAATTCTGAGTAATCTGTA+AGG + chr8.4:54306369-54306388 MS.gene069717:intron 25.0%
!! TATAGGTAATTTTCTCATCT+GGG + chr8.4:54306172-54306191 MS.gene069717:intron 25.0%
!! TTATAGGTAATTTTCTCATC+TGG + chr8.4:54306171-54306190 MS.gene069717:intron 25.0%
!!! GAATAAAGAGTATTGAGAAA+TGG - chr8.4:54305966-54305985 None:intergenic 25.0%
!!! TGGGTTTGATAATTTTTTCA+GGG + chr8.4:54306581-54306600 MS.gene069717:intron 25.0%
!!! TTGGGTTTGATAATTTTTTC+AGG + chr8.4:54306580-54306599 MS.gene069717:intron 25.0%
AAATGTCGATATAGTTAGGT+CGG + chr8.4:54306407-54306426 MS.gene069717:intron 30.0%
AGGTCTTTAACAAAGATCAA+AGG - chr8.4:54306139-54306158 None:intergenic 30.0%
ATACCAGTAAATGTGTTCAA+AGG - chr8.4:54306627-54306646 None:intergenic 30.0%
ATCAATATCACATTCTCTGA+AGG - chr8.4:54306741-54306760 None:intergenic 30.0%
GTTTGGATGTATACAGAATA+TGG + chr8.4:54306712-54306731 MS.gene069717:CDS 30.0%
TAAACAGAATCAGCATCAAA+AGG + chr8.4:54306060-54306079 MS.gene069717:CDS 30.0%
TACCAGTAAATGTGTTCAAA+GGG - chr8.4:54306626-54306645 None:intergenic 30.0%
TGGTAAATGTCGATATAGTT+AGG + chr8.4:54306403-54306422 MS.gene069717:intron 30.0%
TGTTTATCTTGAGTAGCTAA+TGG - chr8.4:54306046-54306065 None:intergenic 30.0%
! TCCAAATCCAACATTTTCAT+GGG + chr8.4:54306953-54306972 MS.gene069717:CDS 30.0%
! TGGATTTTTAATGTTGGGTT+TGG + chr8.4:54306271-54306290 MS.gene069717:intron 30.0%
! TTGGTGTTTGAATCTTGATT+TGG - chr8.4:54305924-54305943 None:intergenic 30.0%
!! AGAATTTTGTTTCGTGAGAT+TGG + chr8.4:54306690-54306709 MS.gene069717:CDS 30.0%
!! GATGAGTTTTATCTCAATCT+TGG + chr8.4:54306082-54306101 MS.gene069717:CDS 30.0%
!! TGTTGGGTTTGGTAATTTTA+AGG + chr8.4:54306282-54306301 MS.gene069717:intron 30.0%
!! TTGAATCTTGATTTGGATTG+AGG - chr8.4:54305917-54305936 None:intergenic 30.0%
!!! AGAAGTACAAGTTGATCTTT+TGG + chr8.4:54306649-54306668 MS.gene069717:CDS 30.0%
!!! GAAGTACAAGTTGATCTTTT+GGG + chr8.4:54306650-54306669 MS.gene069717:CDS 30.0%
ACCAATATATGCACATCCAA+AGG - chr8.4:54306332-54306351 None:intergenic 35.0%
AGAATGTGATATTGATCAGG+TGG + chr8.4:54306745-54306764 MS.gene069717:CDS 35.0%
CAGAGAATGTGATATTGATC+AGG + chr8.4:54306742-54306761 MS.gene069717:CDS 35.0%
CCAATATATGCACATCCAAA+GGG - chr8.4:54306331-54306350 None:intergenic 35.0%
GAAAGATTGAGATTGCAAAG+AGG - chr8.4:54305887-54305906 None:intergenic 35.0%
GAACATGAACTAGAATCCAA+AGG - chr8.4:54306981-54307000 None:intergenic 35.0%
GAAGCTAAAGGTGAATTTCA+AGG + chr8.4:54306798-54306817 MS.gene069717:CDS 35.0%
! CAGGGATGATGTAACATTTT+TGG + chr8.4:54306599-54306618 MS.gene069717:intron 35.0%
! CCAAATCCAACATTTTCATG+GGG + chr8.4:54306954-54306973 MS.gene069717:CDS 35.0%
! GTCCAAATCCAACATTTTCA+TGG + chr8.4:54306952-54306971 MS.gene069717:CDS 35.0%
! TTTGTTTCGTGAGATTGGTT+TGG + chr8.4:54306695-54306714 MS.gene069717:CDS 35.0%
!! TGGATTCTAGTTCATGTTCA+TGG + chr8.4:54306982-54307001 MS.gene069717:CDS 35.0%
!!! GTTTTGAAGAAGACAGTGTT+TGG - chr8.4:54305943-54305962 None:intergenic 35.0%
AAAGGACCCCATGAAAATGT+TGG - chr8.4:54306963-54306982 None:intergenic 40.0%
ACCAAATCCAAAACCGAAAC+CGG - chr8.4:54306915-54306934 None:intergenic 40.0%
AGGTACTTCGCGGTATAAAT+TGG + chr8.4:54306818-54306837 MS.gene069717:CDS 40.0%
ATTGATCAGGTGGAACTTGA+GGG + chr8.4:54306755-54306774 MS.gene069717:CDS 40.0%
CCCCATGAAAATGTTGGATT+TGG - chr8.4:54306957-54306976 None:intergenic 40.0%
CCCTTTGGATGTGCATATAT+TGG + chr8.4:54306328-54306347 MS.gene069717:intron 40.0%
CGGTATAAATTGGATCGGAA+CGG + chr8.4:54306828-54306847 MS.gene069717:CDS 40.0%
CTTCGCGGTATAAATTGGAT+CGG + chr8.4:54306823-54306842 MS.gene069717:CDS 40.0%
GACCCTTTGAACACATTTAC+TGG + chr8.4:54306621-54306640 MS.gene069717:CDS 40.0%
TATTGATCAGGTGGAACTTG+AGG + chr8.4:54306754-54306773 MS.gene069717:CDS 40.0%
TCAAGCAGTTAAGCACACAT+TGG + chr8.4:54307016-54307035 MS.gene069717:CDS 40.0%
TCCACAGAAGATGATGAAAC+CGG + chr8.4:54306893-54306912 MS.gene069717:CDS 40.0%
! AACATTTTCATGGGGTCCTT+TGG + chr8.4:54306962-54306981 MS.gene069717:CDS 40.0%
! AATTCACCTTTAGCTTCCCA+TGG - chr8.4:54306795-54306814 None:intergenic 40.0%
! GAAGATGATGAAACCGGTTT+CGG + chr8.4:54306899-54306918 MS.gene069717:CDS 40.0%
! GTGAATTTCAAGGTACTTCG+CGG + chr8.4:54306808-54306827 MS.gene069717:CDS 40.0%
!! AATGTTGGATTTGGACTAGC+AGG - chr8.4:54306948-54306967 None:intergenic 40.0%
!! AGTTGATCTTTTGGGCATTG+AGG + chr8.4:54306658-54306677 MS.gene069717:CDS 40.0%
!! ATGTTGGATTTGGACTAGCA+GGG - chr8.4:54306947-54306966 None:intergenic 40.0%
!!! TTTTGGGCATTGAGGTTTCA+TGG + chr8.4:54306666-54306685 MS.gene069717:CDS 40.0%
AGACAGTTGTTACCACCAGA+TGG + chr8.4:54307047-54307066 MS.gene069717:CDS 45.0%
AGTCCAAAGTTTCACTGCAC+AGG + chr8.4:54306003-54306022 MS.gene069717:CDS 45.0%
CAACCTGTGCAGTGAAACTT+TGG - chr8.4:54306009-54306028 None:intergenic 45.0%
CGACATTTACCAGTTGAGCT+AGG - chr8.4:54306395-54306414 None:intergenic 45.0%
GCAGTGAAACTTTGGACTGA+AGG - chr8.4:54306001-54306020 None:intergenic 45.0%
GTTGTTACCACCAGATGGTT+TGG + chr8.4:54307052-54307071 MS.gene069717:CDS 45.0%
TTGATCAGGTGGAACTTGAG+GGG + chr8.4:54306756-54306775 MS.gene069717:CDS 45.0%
! ACCGGTTTCATCATCTTCTG+TGG - chr8.4:54306897-54306916 None:intergenic 45.0%
! CAAAGGCATATCCTCACGAA+GGG - chr8.4:54306122-54306141 None:intergenic 45.0%
! TCAAAGGCATATCCTCACGA+AGG - chr8.4:54306123-54306142 None:intergenic 45.0%
!! GATGAAACCGGTTTCGGTTT+TGG + chr8.4:54306905-54306924 MS.gene069717:CDS 45.0%
!!! ACCGGTTTCGGTTTTGGATT+TGG + chr8.4:54306911-54306930 MS.gene069717:CDS 45.0%
AGGGTTCCATGGGAAGCTAA+AGG + chr8.4:54306786-54306805 MS.gene069717:CDS 50.0%
AGTCGTTCGAACCCAGGTTA+AGG - chr8.4:54306436-54306455 None:intergenic 50.0%
GAACATGCAGCCAAACCATC+TGG - chr8.4:54307065-54307084 None:intergenic 50.0%
TAGGTCGGACACCTTAACCT+GGG + chr8.4:54306422-54306441 MS.gene069717:intron 50.0%
TCTGTAAGGCCTAGCTCAAC+TGG + chr8.4:54306383-54306402 MS.gene069717:intron 50.0%
TGCTGTTAGAACCCTTCGTG+AGG + chr8.4:54306108-54306127 MS.gene069717:CDS 50.0%
TTAGCTTCCCATGGAACCCT+AGG - chr8.4:54306786-54306805 None:intergenic 50.0%
TTAGGTCGGACACCTTAACC+TGG + chr8.4:54306421-54306440 MS.gene069717:intron 50.0%
TTTCACTGCACAGGTTGCTC+AGG + chr8.4:54306012-54306031 MS.gene069717:CDS 50.0%
CATGCAGCCAAACCATCTGG+TGG - chr8.4:54307062-54307081 None:intergenic 55.0%
TCGAACGACTCGCAGTCAAG+TGG + chr8.4:54306446-54306465 MS.gene069717:intron 55.0%
! GAACTTGAGGGGTGTGCCTA+GGG + chr8.4:54306767-54306786 MS.gene069717:CDS 55.0%
ACTGCGAGTCGTTCGAACCC+AGG - chr8.4:54306442-54306461 None:intergenic 60.0%
GGGTGTGCCTAGGGTTCCAT+GGG + chr8.4:54306776-54306795 MS.gene069717:CDS 60.0%
! GGAACTTGAGGGGTGTGCCT+AGG + chr8.4:54306766-54306785 MS.gene069717:CDS 60.0%
GGGGTGTGCCTAGGGTTCCA+TGG + chr8.4:54306775-54306794 MS.gene069717:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 54305875 54307088 54305875 ID=MS.gene069717
chr8.4 mRNA 54305875 54307088 54305875 ID=MS.gene069717.t1;Parent=MS.gene069717
chr8.4 exon 54305875 54306176 54305875 ID=MS.gene069717.t1.exon1;Parent=MS.gene069717.t1
chr8.4 CDS 54305875 54306176 54305875 ID=cds.MS.gene069717.t1;Parent=MS.gene069717.t1
chr8.4 exon 54306602 54307088 54306602 ID=MS.gene069717.t1.exon2;Parent=MS.gene069717.t1
chr8.4 CDS 54306602 54307088 54306602 ID=cds.MS.gene069717.t1;Parent=MS.gene069717.t1
Gene Sequence

>MS.gene069717

ATGGCTTTTCCTCTTTGCAATCTCAATCTTTCTCTATTACCTCAATCCAAATCAAGATTCAAACACCAAACACTGTCTTCTTCAAAACCCATTTCTCAATACTCTTTATTCTTCTCTTCTTCTCCTTCAGTCCAAAGTTTCACTGCACAGGTTGCTCAGGTGAATTCACCATTAGCTACTCAAGATAAACAGAATCAGCATCAAAAGGATGAGTTTTATCTCAATCTTGGTGTTGCTGTTAGAACCCTTCGTGAGGATATGCCTTTGATCTTTGTTAAAGACCTTAATTATGATATTTATAGGTAATTTTCTCATCTGGGTATGTAAAGTTTCTTGCTTTTCATATAAATATATGGTTTTTAATTTGAATTGTGTTATTTCTAGGTGAAAAAAAATTGGATTTTTAATGTTGGGTTTGGTAATTTTAAGGATGATGTAATTAATATTTATGGACCCTTTGGATGTGCATATATTGGTTTTTAATTCGAATTTAGTTAATTCTGAGTAATCTGTAAGGCCTAGCTCAACTGGTAAATGTCGATATAGTTAGGTCGGACACCTTAACCTGGGTTCGAACGACTCGCAGTCAAGTGGTGTATGAGAGTTTAAAACTCATTGCCACTTTCTATGTATAAAAAAAGTTAATTTTGAGTGGAAAAAAATTGGGTTTTTATTCTGTGCAAAAATATAGTTAGAATTTTAATGTTGGGTTTGATAATTTTTTCAGGGATGATGTAACATTTTTGGACCCTTTGAACACATTTACTGGTATTGAGAAGTACAAGTTGATCTTTTGGGCATTGAGGTTTCATGGTAGAATTTTGTTTCGTGAGATTGGTTTGGATGTATACAGAATATGGCAGCCTTCAGAGAATGTGATATTGATCAGGTGGAACTTGAGGGGTGTGCCTAGGGTTCCATGGGAAGCTAAAGGTGAATTTCAAGGTACTTCGCGGTATAAATTGGATCGGAACGGTAAGATTTATGAACACAAAGTTGATAATTTAGCATTCAACTTTCCACAGAAGATGATGAAACCGGTTTCGGTTTTGGATTTGGTTACTGCTTGCCCTGCTAGTCCAAATCCAACATTTTCATGGGGTCCTTTGGATTCTAGTTCATGTTCATGGATAGAATTATATCAAGCAGTTAAGCACACATTGGATGAAGAAAGACAGTTGTTACCACCAGATGGTTTGGCTGCATGTTCATAG

Protein sequence

>MS.gene069717.t1

MAFPLCNLNLSLLPQSKSRFKHQTLSSSKPISQYSLFFSSSPSVQSFTAQVAQVNSPLATQDKQNQHQKDEFYLNLGVAVRTLREDMPLIFVKDLNYDIYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILFREIGLDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQKMMKPVSVLDLVTACPASPNPTFSWGPLDSSSCSWIELYQAVKHTLDEERQLLPPDGLAACS