AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42746


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42746.t1 MTR_8g046460 93.284 268 12 2 1 262 1 268 5.61e-178 490
MS.gene42746.t1 MTR_2g064370 70.522 268 72 4 1 262 1 267 3.79e-131 372
MS.gene42746.t1 MTR_8g028425 57.143 154 63 2 72 223 92 244 5.45e-65 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42746.t1 AT1G16320 69.231 221 67 1 43 262 53 273 4.49e-117 336
MS.gene42746.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene42746.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene42746.t1 AT2G46220 57.325 157 64 2 69 223 81 236 2.50e-66 206

Find 57 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAACCGGTTTCGGTTT+TGG 0.220818 8.2:+54844002 MS.gene42746:CDS
GACCCTTTGAACACATTTAC+TGG 0.240823 8.2:+54843718 MS.gene42746:CDS
AGAAGTACAAGTTGATCTTT+TGG 0.270946 8.2:+54843746 MS.gene42746:CDS
AGGTACTTCGCGGTATAAAT+TGG 0.272271 8.2:+54843915 MS.gene42746:CDS
ACCTTAATTATGATATTTAT+AGG 0.278702 8.2:+54843252 MS.gene42746:CDS
ACCTATAAATATCATAATTA+AGG 0.297853 8.2:-54843253 None:intergenic
CCCCATGAAAATGTTGGATT+TGG 0.313331 8.2:-54844051 None:intergenic
TGGATTCTAGTTCATGTTCA+TGG 0.337564 8.2:+54844079 MS.gene42746:CDS
GAATAAAGAGTATTGAGAAA+TGG 0.346750 8.2:-54843060 None:intergenic
AATAAAGAGTATTGAGAAAT+GGG 0.349742 8.2:-54843059 None:intergenic
GTTTGGATGTATACAGAATA+TGG 0.351747 8.2:+54843809 MS.gene42746:CDS
TTGGTGTTTGAATCTTGATT+TGG 0.370847 8.2:-54843018 None:intergenic
TGTTTATCTTGAGTAGCTAA+TGG 0.394872 8.2:-54843140 None:intergenic
GAAGATGATGAAACCGGTTT+CGG 0.396965 8.2:+54843996 MS.gene42746:CDS
GTTGTTACCACCAGATGGTT+TGG 0.415329 8.2:+54844149 MS.gene42746:CDS
GGAACTTGAGGGGTGTGCCT+AGG 0.417152 8.2:+54843863 MS.gene42746:CDS
TTTGTTTCGTGAGATTGGTT+TGG 0.421815 8.2:+54843792 MS.gene42746:CDS
GAACTTGAGGGGTGTGCCTA+GGG 0.426698 8.2:+54843864 MS.gene42746:CDS
ACCAAATCCAAAACCGAAAC+CGG 0.442141 8.2:-54844009 None:intergenic
CAACCTGTGCAGTGAAACTT+TGG 0.444081 8.2:-54843103 None:intergenic
AATGTTGGATTTGGACTAGC+AGG 0.451399 8.2:-54844042 None:intergenic
TTTCACTGCACAGGTTGCTC+AGG 0.452221 8.2:+54843109 MS.gene42746:CDS
GGGGTGTGCCTAGGGTTCCA+TGG 0.454206 8.2:+54843872 MS.gene42746:CDS
GGGTGTGCCTAGGGTTCCAT+GGG 0.462556 8.2:+54843873 MS.gene42746:CDS
TTGAATCTTGATTTGGATTG+AGG 0.469278 8.2:-54843011 None:intergenic
GAACATGCAGCCAAACCATC+TGG 0.478212 8.2:-54844159 None:intergenic
CAGAGAATGTGATATTGATC+AGG 0.478427 8.2:+54843839 MS.gene42746:CDS
TACCAGTAAATGTGTTCAAA+GGG 0.481927 8.2:-54843720 None:intergenic
AGTCCAAAGTTTCACTGCAC+AGG 0.485891 8.2:+54843100 MS.gene42746:CDS
TCCACAGAAGATGATGAAAC+CGG 0.487224 8.2:+54843990 MS.gene42746:CDS
ATACCAGTAAATGTGTTCAA+AGG 0.496720 8.2:-54843721 None:intergenic
TAAACAGAATCAGCATCAAA+AGG 0.500362 8.2:+54843157 MS.gene42746:CDS
GAAGCTAAAGGTGAATTTCA+AGG 0.517611 8.2:+54843895 MS.gene42746:CDS
GTGAATTTCAAGGTACTTCG+CGG 0.529074 8.2:+54843905 MS.gene42746:CDS
ATCAATATCACATTCTCTGA+AGG 0.533171 8.2:-54843835 None:intergenic
AATTCACCTTTAGCTTCCCA+TGG 0.534577 8.2:-54843889 None:intergenic
TTAGCTTCCCATGGAACCCT+AGG 0.536034 8.2:-54843880 None:intergenic
TATTGATCAGGTGGAACTTG+AGG 0.540071 8.2:+54843851 MS.gene42746:CDS
AAAGGACCCCATGAAAATGT+TGG 0.541364 8.2:-54844057 None:intergenic
ATTGATCAGGTGGAACTTGA+GGG 0.551874 8.2:+54843852 MS.gene42746:CDS
ACCGGTTTCATCATCTTCTG+TGG 0.561359 8.2:-54843991 None:intergenic
AGGGTTCCATGGGAAGCTAA+AGG 0.578430 8.2:+54843883 MS.gene42746:CDS
GAAAGATTGAGATTGCAAAG+AGG 0.578460 8.2:-54842981 None:intergenic
AGGTCTTTAACAAAGATCAA+AGG 0.587544 8.2:-54843233 None:intergenic
AGACAGTTGTTACCACCAGA+TGG 0.589181 8.2:+54844144 MS.gene42746:CDS
GCAGTGAAACTTTGGACTGA+AGG 0.593412 8.2:-54843095 None:intergenic
AGAATGTGATATTGATCAGG+TGG 0.610163 8.2:+54843842 MS.gene42746:CDS
TCAAGCAGTTAAGCACACAT+TGG 0.624208 8.2:+54844113 MS.gene42746:CDS
CTTCGCGGTATAAATTGGAT+CGG 0.643822 8.2:+54843920 MS.gene42746:CDS
GAACATGAACTAGAATCCAA+AGG 0.647700 8.2:-54844075 None:intergenic
TGCTGTTAGAACCCTTCGTG+AGG 0.651000 8.2:+54843205 MS.gene42746:CDS
CGGTATAAATTGGATCGGAA+CGG 0.651485 8.2:+54843925 MS.gene42746:CDS
TTGATCAGGTGGAACTTGAG+GGG 0.654298 8.2:+54843853 MS.gene42746:CDS
CAAAGGCATATCCTCACGAA+GGG 0.657035 8.2:-54843216 None:intergenic
ATGTTGGATTTGGACTAGCA+GGG 0.662829 8.2:-54844041 None:intergenic
TCAAAGGCATATCCTCACGA+AGG 0.664210 8.2:-54843217 None:intergenic
CATGCAGCCAAACCATCTGG+TGG 0.695452 8.2:-54844156 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTATAAATATCATAATTA+AGG - chr8.2:54843256-54843275 None:intergenic 15.0%
!! ACCTTAATTATGATATTTAT+AGG + chr8.2:54843252-54843271 MS.gene42746:CDS 15.0%
!! GATGATGTAATTAATATTTA+TGG + chr8.2:54843401-54843420 MS.gene42746:intron 15.0%
!!! AAAAATTGGATTTTTAATGT+TGG + chr8.2:54843362-54843381 MS.gene42746:intron 15.0%
!!! AAAATTGGATTTTTAATGTT+GGG + chr8.2:54843363-54843382 MS.gene42746:intron 15.0%
!!! ATAGTTAGAATTTTAATGTT+GGG + chr8.2:54843659-54843678 MS.gene42746:intron 15.0%
!!! TAAAAAAAGTTAATTTTGAG+TGG + chr8.2:54843604-54843623 MS.gene42746:intron 15.0%
!!! TATAGTTAGAATTTTAATGT+TGG + chr8.2:54843658-54843677 MS.gene42746:intron 15.0%
!!! TTGCTTTTCATATAAATATA+TGG + chr8.2:54843305-54843324 MS.gene42746:intron 15.0%
!!! AATAAAGAGTATTGAGAAAT+GGG - chr8.2:54843062-54843081 None:intergenic 20.0%
!!! AATTTTGAGTGGAAAAAAAT+TGG + chr8.2:54843615-54843634 MS.gene42746:intron 20.0%
!!! ATTTCTAGGTGAAAAAAAAT+TGG + chr8.2:54843348-54843367 MS.gene42746:intron 20.0%
!!! ATTTGAATTGTGTTATTTCT+AGG + chr8.2:54843334-54843353 MS.gene42746:intron 20.0%
!!! ATTTTGAGTGGAAAAAAATT+GGG + chr8.2:54843616-54843635 MS.gene42746:intron 20.0%
!!! CTTTTTTTATACATAGAAAG+TGG - chr8.2:54843593-54843612 None:intergenic 20.0%
! ATTAATATTTATGGACCCTT+TGG + chr8.2:54843410-54843429 MS.gene42746:intron 25.0%
! TTAATTCTGAGTAATCTGTA+AGG + chr8.2:54843466-54843485 MS.gene42746:intron 25.0%
!! TATAGGTAATTTTCTCATCT+GGG + chr8.2:54843269-54843288 MS.gene42746:intron 25.0%
!! TTATAGGTAATTTTCTCATC+TGG + chr8.2:54843268-54843287 MS.gene42746:intron 25.0%
!!! GAATAAAGAGTATTGAGAAA+TGG - chr8.2:54843063-54843082 None:intergenic 25.0%
!!! TGGGTTTGATAATTTTTTCA+GGG + chr8.2:54843678-54843697 MS.gene42746:intron 25.0%
!!! TTGGGTTTGATAATTTTTTC+AGG + chr8.2:54843677-54843696 MS.gene42746:intron 25.0%
AAATGTCGATATAGTTAGGT+CGG + chr8.2:54843504-54843523 MS.gene42746:intron 30.0%
AGGTCTTTAACAAAGATCAA+AGG - chr8.2:54843236-54843255 None:intergenic 30.0%
ATACCAGTAAATGTGTTCAA+AGG - chr8.2:54843724-54843743 None:intergenic 30.0%
ATCAATATCACATTCTCTGA+AGG - chr8.2:54843838-54843857 None:intergenic 30.0%
GTTTGGATGTATACAGAATA+TGG + chr8.2:54843809-54843828 MS.gene42746:CDS 30.0%
TAAACAGAATCAGCATCAAA+AGG + chr8.2:54843157-54843176 MS.gene42746:CDS 30.0%
TACCAGTAAATGTGTTCAAA+GGG - chr8.2:54843723-54843742 None:intergenic 30.0%
TGGTAAATGTCGATATAGTT+AGG + chr8.2:54843500-54843519 MS.gene42746:intron 30.0%
TGTTTATCTTGAGTAGCTAA+TGG - chr8.2:54843143-54843162 None:intergenic 30.0%
! TCCAAATCCAACATTTTCAT+GGG + chr8.2:54844050-54844069 MS.gene42746:CDS 30.0%
! TGGATTTTTAATGTTGGGTT+TGG + chr8.2:54843368-54843387 MS.gene42746:intron 30.0%
! TTGGTGTTTGAATCTTGATT+TGG - chr8.2:54843021-54843040 None:intergenic 30.0%
!! AGAATTTTGTTTCGTGAGAT+TGG + chr8.2:54843787-54843806 MS.gene42746:CDS 30.0%
!! GATGAGTTTTATCTCAATCT+TGG + chr8.2:54843179-54843198 MS.gene42746:CDS 30.0%
!! TGTTGGGTTTGGTAATTTTA+AGG + chr8.2:54843379-54843398 MS.gene42746:intron 30.0%
!! TTGAATCTTGATTTGGATTG+AGG - chr8.2:54843014-54843033 None:intergenic 30.0%
!!! AGAAGTACAAGTTGATCTTT+TGG + chr8.2:54843746-54843765 MS.gene42746:CDS 30.0%
!!! GAAGTACAAGTTGATCTTTT+GGG + chr8.2:54843747-54843766 MS.gene42746:CDS 30.0%
ACCAATATATGCACATCCAA+AGG - chr8.2:54843429-54843448 None:intergenic 35.0%
AGAATGTGATATTGATCAGG+TGG + chr8.2:54843842-54843861 MS.gene42746:CDS 35.0%
CAGAGAATGTGATATTGATC+AGG + chr8.2:54843839-54843858 MS.gene42746:CDS 35.0%
CCAATATATGCACATCCAAA+GGG - chr8.2:54843428-54843447 None:intergenic 35.0%
GAAAGATTGAGATTGCAAAG+AGG - chr8.2:54842984-54843003 None:intergenic 35.0%
GAACATGAACTAGAATCCAA+AGG - chr8.2:54844078-54844097 None:intergenic 35.0%
GAAGCTAAAGGTGAATTTCA+AGG + chr8.2:54843895-54843914 MS.gene42746:CDS 35.0%
! CAGGGATGATGTAACATTTT+TGG + chr8.2:54843696-54843715 MS.gene42746:intron 35.0%
! CCAAATCCAACATTTTCATG+GGG + chr8.2:54844051-54844070 MS.gene42746:CDS 35.0%
! GTCCAAATCCAACATTTTCA+TGG + chr8.2:54844049-54844068 MS.gene42746:CDS 35.0%
! TTTGTTTCGTGAGATTGGTT+TGG + chr8.2:54843792-54843811 MS.gene42746:CDS 35.0%
!! TGGATTCTAGTTCATGTTCA+TGG + chr8.2:54844079-54844098 MS.gene42746:CDS 35.0%
!!! GTTTTGAAGAAGACAGTGTT+TGG - chr8.2:54843040-54843059 None:intergenic 35.0%
AAAGGACCCCATGAAAATGT+TGG - chr8.2:54844060-54844079 None:intergenic 40.0%
ACCAAATCCAAAACCGAAAC+CGG - chr8.2:54844012-54844031 None:intergenic 40.0%
AGGTACTTCGCGGTATAAAT+TGG + chr8.2:54843915-54843934 MS.gene42746:CDS 40.0%
ATTGATCAGGTGGAACTTGA+GGG + chr8.2:54843852-54843871 MS.gene42746:CDS 40.0%
CCCCATGAAAATGTTGGATT+TGG - chr8.2:54844054-54844073 None:intergenic 40.0%
CCCTTTGGATGTGCATATAT+TGG + chr8.2:54843425-54843444 MS.gene42746:intron 40.0%
CGGTATAAATTGGATCGGAA+CGG + chr8.2:54843925-54843944 MS.gene42746:CDS 40.0%
CTTCGCGGTATAAATTGGAT+CGG + chr8.2:54843920-54843939 MS.gene42746:CDS 40.0%
GACCCTTTGAACACATTTAC+TGG + chr8.2:54843718-54843737 MS.gene42746:CDS 40.0%
TATTGATCAGGTGGAACTTG+AGG + chr8.2:54843851-54843870 MS.gene42746:CDS 40.0%
TCAAGCAGTTAAGCACACAT+TGG + chr8.2:54844113-54844132 MS.gene42746:CDS 40.0%
TCCACAGAAGATGATGAAAC+CGG + chr8.2:54843990-54844009 MS.gene42746:CDS 40.0%
! AACATTTTCATGGGGTCCTT+TGG + chr8.2:54844059-54844078 MS.gene42746:CDS 40.0%
! AATTCACCTTTAGCTTCCCA+TGG - chr8.2:54843892-54843911 None:intergenic 40.0%
! GAAGATGATGAAACCGGTTT+CGG + chr8.2:54843996-54844015 MS.gene42746:CDS 40.0%
! GTGAATTTCAAGGTACTTCG+CGG + chr8.2:54843905-54843924 MS.gene42746:CDS 40.0%
!! AATGTTGGATTTGGACTAGC+AGG - chr8.2:54844045-54844064 None:intergenic 40.0%
!! AGTTGATCTTTTGGGCATTG+AGG + chr8.2:54843755-54843774 MS.gene42746:CDS 40.0%
!! ATGTTGGATTTGGACTAGCA+GGG - chr8.2:54844044-54844063 None:intergenic 40.0%
!!! TTTTGGGCATTGAGGTTTCA+TGG + chr8.2:54843763-54843782 MS.gene42746:CDS 40.0%
AGACAGTTGTTACCACCAGA+TGG + chr8.2:54844144-54844163 MS.gene42746:CDS 45.0%
AGTCCAAAGTTTCACTGCAC+AGG + chr8.2:54843100-54843119 MS.gene42746:CDS 45.0%
CAACCTGTGCAGTGAAACTT+TGG - chr8.2:54843106-54843125 None:intergenic 45.0%
CGACATTTACCAGTTGAGCT+AGG - chr8.2:54843492-54843511 None:intergenic 45.0%
GCAGTGAAACTTTGGACTGA+AGG - chr8.2:54843098-54843117 None:intergenic 45.0%
GTTGTTACCACCAGATGGTT+TGG + chr8.2:54844149-54844168 MS.gene42746:CDS 45.0%
TTGATCAGGTGGAACTTGAG+GGG + chr8.2:54843853-54843872 MS.gene42746:CDS 45.0%
! ACCGGTTTCATCATCTTCTG+TGG - chr8.2:54843994-54844013 None:intergenic 45.0%
! CAAAGGCATATCCTCACGAA+GGG - chr8.2:54843219-54843238 None:intergenic 45.0%
! TCAAAGGCATATCCTCACGA+AGG - chr8.2:54843220-54843239 None:intergenic 45.0%
!! GATGAAACCGGTTTCGGTTT+TGG + chr8.2:54844002-54844021 MS.gene42746:CDS 45.0%
!!! ACCGGTTTCGGTTTTGGATT+TGG + chr8.2:54844008-54844027 MS.gene42746:CDS 45.0%
AGGGTTCCATGGGAAGCTAA+AGG + chr8.2:54843883-54843902 MS.gene42746:CDS 50.0%
AGTCGTTCGAACCCAGGTTA+AGG - chr8.2:54843533-54843552 None:intergenic 50.0%
GAACATGCAGCCAAACCATC+TGG - chr8.2:54844162-54844181 None:intergenic 50.0%
TAGGTCGGACACCTTAACCT+GGG + chr8.2:54843519-54843538 MS.gene42746:intron 50.0%
TCTGTAAGGCCTAGCTCAAC+TGG + chr8.2:54843480-54843499 MS.gene42746:intron 50.0%
TGCTGTTAGAACCCTTCGTG+AGG + chr8.2:54843205-54843224 MS.gene42746:CDS 50.0%
TTAGCTTCCCATGGAACCCT+AGG - chr8.2:54843883-54843902 None:intergenic 50.0%
TTAGGTCGGACACCTTAACC+TGG + chr8.2:54843518-54843537 MS.gene42746:intron 50.0%
TTTCACTGCACAGGTTGCTC+AGG + chr8.2:54843109-54843128 MS.gene42746:CDS 50.0%
CATGCAGCCAAACCATCTGG+TGG - chr8.2:54844159-54844178 None:intergenic 55.0%
TCGAACGACTCGCAGTCAAG+TGG + chr8.2:54843543-54843562 MS.gene42746:intron 55.0%
! GAACTTGAGGGGTGTGCCTA+GGG + chr8.2:54843864-54843883 MS.gene42746:CDS 55.0%
ACTGCGAGTCGTTCGAACCC+AGG - chr8.2:54843539-54843558 None:intergenic 60.0%
GGGTGTGCCTAGGGTTCCAT+GGG + chr8.2:54843873-54843892 MS.gene42746:CDS 60.0%
! GGAACTTGAGGGGTGTGCCT+AGG + chr8.2:54843863-54843882 MS.gene42746:CDS 60.0%
GGGGTGTGCCTAGGGTTCCA+TGG + chr8.2:54843872-54843891 MS.gene42746:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 54842972 54844185 54842972 ID=MS.gene42746
chr8.2 mRNA 54842972 54844185 54842972 ID=MS.gene42746.t1;Parent=MS.gene42746
chr8.2 exon 54842972 54843273 54842972 ID=MS.gene42746.t1.exon1;Parent=MS.gene42746.t1
chr8.2 CDS 54842972 54843273 54842972 ID=cds.MS.gene42746.t1;Parent=MS.gene42746.t1
chr8.2 exon 54843699 54844185 54843699 ID=MS.gene42746.t1.exon2;Parent=MS.gene42746.t1
chr8.2 CDS 54843699 54844185 54843699 ID=cds.MS.gene42746.t1;Parent=MS.gene42746.t1
Gene Sequence

>MS.gene42746

ATGGCTTTTCCTCTTTGCAATCTCAATCTTTCTCTATTACCTCAATCCAAATCAAGATTCAAACACCAAACACTGTCTTCTTCAAAACCCATTTCTCAATACTCTTTATTCTTCTCTTCTTCTCCTTCAGTCCAAAGTTTCACTGCACAGGTTGCTCAGGTGAATTCACCATTAGCTACTCAAGATAAACAGAATCAGCATCAAAAGGATGAGTTTTATCTCAATCTTGGTGTTGCTGTTAGAACCCTTCGTGAGGATATGCCTTTGATCTTTGTTAAAGACCTTAATTATGATATTTATAGGTAATTTTCTCATCTGGGTATGTAAAGTTTCTTGCTTTTCATATAAATATATGGTTTTTAATTTGAATTGTGTTATTTCTAGGTGAAAAAAAATTGGATTTTTAATGTTGGGTTTGGTAATTTTAAGGATGATGTAATTAATATTTATGGACCCTTTGGATGTGCATATATTGGTTTTTAATTCGAATTTAGTTAATTCTGAGTAATCTGTAAGGCCTAGCTCAACTGGTAAATGTCGATATAGTTAGGTCGGACACCTTAACCTGGGTTCGAACGACTCGCAGTCAAGTGGTGTATGAGAGTTTAAAACTCATTGCCACTTTCTATGTATAAAAAAAGTTAATTTTGAGTGGAAAAAAATTGGGTTTTTATTCTGTGCAAAAATATAGTTAGAATTTTAATGTTGGGTTTGATAATTTTTTCAGGGATGATGTAACATTTTTGGACCCTTTGAACACATTTACTGGTATTGAGAAGTACAAGTTGATCTTTTGGGCATTGAGGTTTCATGGTAGAATTTTGTTTCGTGAGATTGGTTTGGATGTATACAGAATATGGCAGCCTTCAGAGAATGTGATATTGATCAGGTGGAACTTGAGGGGTGTGCCTAGGGTTCCATGGGAAGCTAAAGGTGAATTTCAAGGTACTTCGCGGTATAAATTGGATCGGAACGGTAAGATTTATGAACACAAAGTTGATAATTTAGCATTCAACTTTCCACAGAAGATGATGAAACCGGTTTCGGTTTTGGATTTGGTTACTGCTTGCCCTGCTAGTCCAAATCCAACATTTTCATGGGGTCCTTTGGATTCTAGTTCATGTTCATGGATAGAATTATATCAAGCAGTTAAGCACACATTGGATGAAGAAAGACAGTTGTTACCACCAGATGGTTTGGCTGCATGTTCATAG

Protein sequence

>MS.gene42746.t1

MAFPLCNLNLSLLPQSKSRFKHQTLSSSKPISQYSLFFSSSPSVQSFTAQVAQVNSPLATQDKQNQHQKDEFYLNLGVAVRTLREDMPLIFVKDLNYDIYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILFREIGLDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQKMMKPVSVLDLVTACPASPNPTFSWGPLDSSSCSWIELYQAVKHTLDEERQLLPPDGLAACS