AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36105


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36105.t1 MTR_8g046460 93.284 268 12 2 1 262 1 268 1.13e-177 489
MS.gene36105.t1 MTR_2g064370 70.632 269 70 5 1 262 1 267 6.34e-131 372
MS.gene36105.t1 MTR_8g028425 57.143 154 63 2 72 223 92 244 6.34e-65 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36105.t1 AT1G16320 69.545 220 66 1 44 262 54 273 4.07e-117 337
MS.gene36105.t1 AT1G79510 61.484 283 80 6 1 262 1 275 1.19e-116 335
MS.gene36105.t1 AT1G79510 61.484 283 80 6 1 262 1 275 1.19e-116 335
MS.gene36105.t1 AT2G46220 57.325 157 64 2 69 223 81 236 3.25e-66 206

Find 57 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAACCGGTTTCGGTTT+TGG 0.220818 8.3:+52626445 MS.gene36105:CDS
GACCCTTTGAACACATTTAC+TGG 0.240823 8.3:+52626161 MS.gene36105:CDS
AGGTACTTCTCGGTATAAAT+TGG 0.261999 8.3:+52626358 MS.gene36105:CDS
AGAAGTACAAGTTGATCTTT+TGG 0.270946 8.3:+52626189 MS.gene36105:CDS
ACCTTAATTATGATATTTAT+AGG 0.278702 8.3:+52625702 MS.gene36105:CDS
ACCTATAAATATCATAATTA+AGG 0.297853 8.3:-52625703 None:intergenic
CCCCATGAAAATGTTGGATT+TGG 0.313331 8.3:-52626494 None:intergenic
TGGATTCTAGTTCATGTTCA+TGG 0.337564 8.3:+52626522 MS.gene36105:CDS
GAATAAAGAGTATTGAGAAA+TGG 0.346750 8.3:-52625510 None:intergenic
AATAAAGAGTATTGAGAAAT+GGG 0.349742 8.3:-52625509 None:intergenic
GTTTGGATGTATACAGAATA+TGG 0.351747 8.3:+52626252 MS.gene36105:CDS
TTGGTGTTTGAATCTTGATT+TGG 0.370847 8.3:-52625468 None:intergenic
TGTTTATCTTGAGTAGCTAA+TGG 0.394872 8.3:-52625590 None:intergenic
GAAGATGATGAAACCGGTTT+CGG 0.396965 8.3:+52626439 MS.gene36105:CDS
GTTGTTACCACCAGATGGTT+TGG 0.412534 8.3:+52626592 MS.gene36105:CDS
GTGAATTTCAAGGTACTTCT+CGG 0.412707 8.3:+52626348 MS.gene36105:CDS
GGAACTTGAGGGGTGTGCCT+AGG 0.417152 8.3:+52626306 MS.gene36105:CDS
TTTGTTTCGTGAGATTGGTT+TGG 0.421815 8.3:+52626235 MS.gene36105:CDS
GAACTTGAGGGGTGTGCCTA+GGG 0.426698 8.3:+52626307 MS.gene36105:CDS
ACCAAATCCAAAACCGAAAC+CGG 0.442141 8.3:-52626452 None:intergenic
CAACCTGTGCAGTGAAACTT+TGG 0.444081 8.3:-52625553 None:intergenic
AATGTTGGATTTGGACTAGC+AGG 0.451399 8.3:-52626485 None:intergenic
TTTCACTGCACAGGTTGCTC+AGG 0.452221 8.3:+52625559 MS.gene36105:CDS
GGGGTGTGCCTAGGGTTCCA+TGG 0.454206 8.3:+52626315 MS.gene36105:CDS
GGGTGTGCCTAGGGTTCCAT+GGG 0.462556 8.3:+52626316 MS.gene36105:CDS
TTGAATCTTGATTTGGATTG+AGG 0.469278 8.3:-52625461 None:intergenic
CAGAGAATGTGATATTGATC+AGG 0.478427 8.3:+52626282 MS.gene36105:CDS
TACCAGTAAATGTGTTCAAA+GGG 0.481927 8.3:-52626163 None:intergenic
TCCACAGAAGATGATGAAAC+CGG 0.487224 8.3:+52626433 MS.gene36105:CDS
AGTCCAAAGTTTCACTGCAC+AGG 0.490628 8.3:+52625550 MS.gene36105:CDS
GAACATGTAGCCAAACCATC+TGG 0.493057 8.3:-52626602 None:intergenic
ATACCAGTAAATGTGTTCAA+AGG 0.496720 8.3:-52626164 None:intergenic
GAAGCTAAAGGTGAATTTCA+AGG 0.517611 8.3:+52626338 MS.gene36105:CDS
GCAGTGAAACTTTGGACTAA+AGG 0.527370 8.3:-52625545 None:intergenic
ATCAATATCACATTCTCTGA+AGG 0.533171 8.3:-52626278 None:intergenic
AATTCACCTTTAGCTTCCCA+TGG 0.534577 8.3:-52626332 None:intergenic
TTAGCTTCCCATGGAACCCT+AGG 0.536034 8.3:-52626323 None:intergenic
TATTGATCAGGTGGAACTTG+AGG 0.540071 8.3:+52626294 MS.gene36105:CDS
AAAGGACCCCATGAAAATGT+TGG 0.541364 8.3:-52626500 None:intergenic
ATTGATCAGGTGGAACTTGA+GGG 0.551874 8.3:+52626295 MS.gene36105:CDS
ACCGGTTTCATCATCTTCTG+TGG 0.561359 8.3:-52626434 None:intergenic
GAAAGATTGAGATTGCAAAG+AGG 0.569214 8.3:-52625431 None:intergenic
AGGGTTCCATGGGAAGCTAA+AGG 0.578430 8.3:+52626326 MS.gene36105:CDS
AGGTCTTTAACAAAGATCAA+AGG 0.587544 8.3:-52625683 None:intergenic
AGACAGTTGTTACCACCAGA+TGG 0.589181 8.3:+52626587 MS.gene36105:CDS
TAAACAGAATCAGCATCAGA+AGG 0.609028 8.3:+52625607 MS.gene36105:CDS
AGAATGTGATATTGATCAGG+TGG 0.610163 8.3:+52626285 MS.gene36105:CDS
CTTCTCGGTATAAATTGGAT+CGG 0.610878 8.3:+52626363 MS.gene36105:CDS
TCAAGCAGTTAAGCACACAT+TGG 0.624208 8.3:+52626556 MS.gene36105:CDS
CGGTATAAATTGGATCGGAA+CGG 0.634092 8.3:+52626368 MS.gene36105:CDS
GAACATGAACTAGAATCCAA+AGG 0.647700 8.3:-52626518 None:intergenic
TGCTGTTAGAACCCTTCGTG+AGG 0.651000 8.3:+52625655 MS.gene36105:CDS
TTGATCAGGTGGAACTTGAG+GGG 0.654298 8.3:+52626296 MS.gene36105:CDS
CAAAGGCATATCCTCACGAA+GGG 0.657035 8.3:-52625666 None:intergenic
ATGTTGGATTTGGACTAGCA+GGG 0.662829 8.3:-52626484 None:intergenic
TCAAAGGCATATCCTCACGA+AGG 0.664210 8.3:-52625667 None:intergenic
CATGTAGCCAAACCATCTGG+TGG 0.698747 8.3:-52626599 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTATAAATATCATAATTA+AGG - chr8.3:52625706-52625725 None:intergenic 15.0%
!! ACCTTAATTATGATATTTAT+AGG + chr8.3:52625702-52625721 MS.gene36105:CDS 15.0%
!! GATGATGTAATTAATATTTA+TGG + chr8.3:52625848-52625867 MS.gene36105:intron 15.0%
!!! AAAAATTGGATTTTTAATGT+TGG + chr8.3:52625809-52625828 MS.gene36105:intron 15.0%
!!! AAAATTGGATTTTTAATGTT+GGG + chr8.3:52625810-52625829 MS.gene36105:intron 15.0%
!!! TAAAAAAAGTTAATTTTGAG+TGG + chr8.3:52626050-52626069 MS.gene36105:intron 15.0%
!!! TAATTTGAATTGTGTTATTT+AGG + chr8.3:52625782-52625801 MS.gene36105:intron 15.0%
!! GTTATTTAGGTGAAAAAAAT+TGG + chr8.3:52625795-52625814 MS.gene36105:intron 20.0%
!!! AATAAAGAGTATTGAGAAAT+GGG - chr8.3:52625512-52625531 None:intergenic 20.0%
!!! AATTTTGAGTGGAAAAAAAT+TGG + chr8.3:52626061-52626080 MS.gene36105:intron 20.0%
!!! ATTTTGAGTGGAAAAAAATT+GGG + chr8.3:52626062-52626081 MS.gene36105:intron 20.0%
!!! CTTTTTTTATACATACAAAG+TGG - chr8.3:52626039-52626058 None:intergenic 20.0%
!!! GTGAATATATGGTTTTTAAT+TGG + chr8.3:52625882-52625901 MS.gene36105:intron 20.0%
! ACCATATATTCACATCTAAA+AGG - chr8.3:52625875-52625894 None:intergenic 25.0%
! TTGCTTTCGATGTAAATATA+TGG + chr8.3:52625755-52625774 MS.gene36105:intron 25.0%
!! ACCTTTTAGATGTGAATATA+TGG + chr8.3:52625871-52625890 MS.gene36105:intron 25.0%
!! TATAGGTAATTTTCTCATCT+GGG + chr8.3:52625719-52625738 MS.gene36105:intron 25.0%
!! TGTTGGGTTTAGTAATTTTA+AGG + chr8.3:52625826-52625845 MS.gene36105:intron 25.0%
!! TTATAGGTAATTTTCTCATC+TGG + chr8.3:52625718-52625737 MS.gene36105:intron 25.0%
!! TTATTTTCTGCAAAAAGAGT+TGG + chr8.3:52626088-52626107 MS.gene36105:intron 25.0%
!!! AAGAGTTGGAATTTTAATGT+TGG + chr8.3:52626102-52626121 MS.gene36105:intron 25.0%
!!! AGAGTTGGAATTTTAATGTT+GGG + chr8.3:52626103-52626122 MS.gene36105:intron 25.0%
!!! GAATAAAGAGTATTGAGAAA+TGG - chr8.3:52625513-52625532 None:intergenic 25.0%
!!! TTGGGTTTGATAATTTTTCA+GGG + chr8.3:52626121-52626140 MS.gene36105:intron 25.0%
AGGTCTTTAACAAAGATCAA+AGG - chr8.3:52625686-52625705 None:intergenic 30.0%
ATACCAGTAAATGTGTTCAA+AGG - chr8.3:52626167-52626186 None:intergenic 30.0%
ATCAATATCACATTCTCTGA+AGG - chr8.3:52626281-52626300 None:intergenic 30.0%
GTTTGGATGTATACAGAATA+TGG + chr8.3:52626252-52626271 MS.gene36105:CDS 30.0%
TACCAGTAAATGTGTTCAAA+GGG - chr8.3:52626166-52626185 None:intergenic 30.0%
TGTTTATCTTGAGTAGCTAA+TGG - chr8.3:52625593-52625612 None:intergenic 30.0%
! CTAATTCTGAGTAACTTGTA+AGG + chr8.3:52625912-52625931 MS.gene36105:intron 30.0%
! TCCAAATCCAACATTTTCAT+GGG + chr8.3:52626493-52626512 MS.gene36105:CDS 30.0%
! TTGGTGTTTGAATCTTGATT+TGG - chr8.3:52625471-52625490 None:intergenic 30.0%
!! AGAATTTTGTTTCGTGAGAT+TGG + chr8.3:52626230-52626249 MS.gene36105:CDS 30.0%
!! GATGAGTTTTATCTCAATCT+TGG + chr8.3:52625629-52625648 MS.gene36105:CDS 30.0%
!! GTTGGGTTTGATAATTTTTC+AGG + chr8.3:52626120-52626139 MS.gene36105:intron 30.0%
!! TTGAATCTTGATTTGGATTG+AGG - chr8.3:52625464-52625483 None:intergenic 30.0%
!!! AGAAGTACAAGTTGATCTTT+TGG + chr8.3:52626189-52626208 MS.gene36105:CDS 30.0%
!!! GAAGTACAAGTTGATCTTTT+GGG + chr8.3:52626190-52626209 MS.gene36105:CDS 30.0%
!!! GTTTTGAAGAAGAAAGTGTT+TGG - chr8.3:52625490-52625509 None:intergenic 30.0%
AGAATGTGATATTGATCAGG+TGG + chr8.3:52626285-52626304 MS.gene36105:CDS 35.0%
AGGTACTTCTCGGTATAAAT+TGG + chr8.3:52626358-52626377 MS.gene36105:CDS 35.0%
CAGAGAATGTGATATTGATC+AGG + chr8.3:52626282-52626301 MS.gene36105:CDS 35.0%
CTTCTCGGTATAAATTGGAT+CGG + chr8.3:52626363-52626382 MS.gene36105:CDS 35.0%
GAAAGATTGAGATTGCAAAG+AGG - chr8.3:52625434-52625453 None:intergenic 35.0%
GAACATGAACTAGAATCCAA+AGG - chr8.3:52626521-52626540 None:intergenic 35.0%
GAAGCTAAAGGTGAATTTCA+AGG + chr8.3:52626338-52626357 MS.gene36105:CDS 35.0%
TAAACAGAATCAGCATCAGA+AGG + chr8.3:52625607-52625626 MS.gene36105:CDS 35.0%
! CAGGGATGATGTAACATTTT+TGG + chr8.3:52626139-52626158 MS.gene36105:intron 35.0%
! CCAAATCCAACATTTTCATG+GGG + chr8.3:52626494-52626513 MS.gene36105:CDS 35.0%
! GTCCAAATCCAACATTTTCA+TGG + chr8.3:52626492-52626511 MS.gene36105:CDS 35.0%
! GTGAATTTCAAGGTACTTCT+CGG + chr8.3:52626348-52626367 MS.gene36105:CDS 35.0%
! TTTGTTTCGTGAGATTGGTT+TGG + chr8.3:52626235-52626254 MS.gene36105:CDS 35.0%
!! TGGATTCTAGTTCATGTTCA+TGG + chr8.3:52626522-52626541 MS.gene36105:CDS 35.0%
AAAGGACCCCATGAAAATGT+TGG - chr8.3:52626503-52626522 None:intergenic 40.0%
AAATGCCGATATAGTTAGGC+TGG + chr8.3:52625950-52625969 MS.gene36105:intron 40.0%
ACCAAATCCAAAACCGAAAC+CGG - chr8.3:52626455-52626474 None:intergenic 40.0%
ATTGATCAGGTGGAACTTGA+GGG + chr8.3:52626295-52626314 MS.gene36105:CDS 40.0%
CCCCATGAAAATGTTGGATT+TGG - chr8.3:52626497-52626516 None:intergenic 40.0%
CGGTATAAATTGGATCGGAA+CGG + chr8.3:52626368-52626387 MS.gene36105:CDS 40.0%
GACCCTTTGAACACATTTAC+TGG + chr8.3:52626161-52626180 MS.gene36105:CDS 40.0%
GCAGTGAAACTTTGGACTAA+AGG - chr8.3:52625548-52625567 None:intergenic 40.0%
TATTGATCAGGTGGAACTTG+AGG + chr8.3:52626294-52626313 MS.gene36105:CDS 40.0%
TCAAGCAGTTAAGCACACAT+TGG + chr8.3:52626556-52626575 MS.gene36105:CDS 40.0%
TCCACAGAAGATGATGAAAC+CGG + chr8.3:52626433-52626452 MS.gene36105:CDS 40.0%
TGGCAAATGCCGATATAGTT+AGG + chr8.3:52625946-52625965 MS.gene36105:intron 40.0%
! AACATTTTCATGGGGTCCTT+TGG + chr8.3:52626502-52626521 MS.gene36105:CDS 40.0%
! AATTCACCTTTAGCTTCCCA+TGG - chr8.3:52626335-52626354 None:intergenic 40.0%
! GAAGATGATGAAACCGGTTT+CGG + chr8.3:52626439-52626458 MS.gene36105:CDS 40.0%
!! AATGTTGGATTTGGACTAGC+AGG - chr8.3:52626488-52626507 None:intergenic 40.0%
!! AGTTGATCTTTTGGGCATTG+AGG + chr8.3:52626198-52626217 MS.gene36105:CDS 40.0%
!! ATGTTGGATTTGGACTAGCA+GGG - chr8.3:52626487-52626506 None:intergenic 40.0%
!!! TTTTGGGCATTGAGGTTTCA+TGG + chr8.3:52626206-52626225 MS.gene36105:CDS 40.0%
AGACAGTTGTTACCACCAGA+TGG + chr8.3:52626587-52626606 MS.gene36105:CDS 45.0%
AGTCCAAAGTTTCACTGCAC+AGG + chr8.3:52625550-52625569 MS.gene36105:CDS 45.0%
CAACCTGTGCAGTGAAACTT+TGG - chr8.3:52625556-52625575 None:intergenic 45.0%
GAACATGTAGCCAAACCATC+TGG - chr8.3:52626605-52626624 None:intergenic 45.0%
GGTGTCCAGCCTAACTATAT+CGG - chr8.3:52625958-52625977 None:intergenic 45.0%
GTTGTTACCACCAGATGGTT+TGG + chr8.3:52626592-52626611 MS.gene36105:CDS 45.0%
TTGATCAGGTGGAACTTGAG+GGG + chr8.3:52626296-52626315 MS.gene36105:CDS 45.0%
! ACCGGTTTCATCATCTTCTG+TGG - chr8.3:52626437-52626456 None:intergenic 45.0%
! CAAAGGCATATCCTCACGAA+GGG - chr8.3:52625669-52625688 None:intergenic 45.0%
! TCAAAGGCATATCCTCACGA+AGG - chr8.3:52625670-52625689 None:intergenic 45.0%
!! GATGAAACCGGTTTCGGTTT+TGG + chr8.3:52626445-52626464 MS.gene36105:CDS 45.0%
!!! ACCGGTTTCGGTTTTGGATT+TGG + chr8.3:52626451-52626470 MS.gene36105:CDS 45.0%
ACTACGAGTCGTTCGAACCT+AGG - chr8.3:52625985-52626004 None:intergenic 50.0%
AGGGTTCCATGGGAAGCTAA+AGG + chr8.3:52626326-52626345 MS.gene36105:CDS 50.0%
AGTCGTTCGAACCTAGGTGA+AGG - chr8.3:52625979-52625998 None:intergenic 50.0%
CATGTAGCCAAACCATCTGG+TGG - chr8.3:52626602-52626621 None:intergenic 50.0%
CTTGTAAGGCCTAGCTCAAC+TGG + chr8.3:52625926-52625945 MS.gene36105:intron 50.0%
TAGGTTCGAACGACTCGTAG+TGG + chr8.3:52625984-52626003 MS.gene36105:intron 50.0%
TGCTGTTAGAACCCTTCGTG+AGG + chr8.3:52625655-52625674 MS.gene36105:CDS 50.0%
TTAGCTTCCCATGGAACCCT+AGG - chr8.3:52626326-52626345 None:intergenic 50.0%
TTTCACTGCACAGGTTGCTC+AGG + chr8.3:52625559-52625578 MS.gene36105:CDS 50.0%
CGGCATTTGCCAGTTGAGCT+AGG - chr8.3:52625938-52625957 None:intergenic 55.0%
TAGGCTGGACACCTTCACCT+AGG + chr8.3:52625965-52625984 MS.gene36105:intron 55.0%
! GAACTTGAGGGGTGTGCCTA+GGG + chr8.3:52626307-52626326 MS.gene36105:CDS 55.0%
! TCGAACGACTCGTAGTGGAG+TGG + chr8.3:52625989-52626008 MS.gene36105:intron 55.0%
GGGTGTGCCTAGGGTTCCAT+GGG + chr8.3:52626316-52626335 MS.gene36105:CDS 60.0%
! GGAACTTGAGGGGTGTGCCT+AGG + chr8.3:52626306-52626325 MS.gene36105:CDS 60.0%
GGGGTGTGCCTAGGGTTCCA+TGG + chr8.3:52626315-52626334 MS.gene36105:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 52625422 52626628 52625422 ID=MS.gene36105
chr8.3 mRNA 52625422 52626628 52625422 ID=MS.gene36105.t1;Parent=MS.gene36105
chr8.3 exon 52625422 52625723 52625422 ID=MS.gene36105.t1.exon1;Parent=MS.gene36105.t1
chr8.3 CDS 52625422 52625723 52625422 ID=cds.MS.gene36105.t1;Parent=MS.gene36105.t1
chr8.3 exon 52626142 52626628 52626142 ID=MS.gene36105.t1.exon2;Parent=MS.gene36105.t1
chr8.3 CDS 52626142 52626628 52626142 ID=cds.MS.gene36105.t1;Parent=MS.gene36105.t1
Gene Sequence

>MS.gene36105

ATGGCTTTTCCTCTTTGCAATCTCAATCTTTCTCTTTTACCTCAATCCAAATCAAGATTCAAACACCAAACACTTTCTTCTTCAAAACCCATTTCTCAATACTCTTTATTCTTCTCTTCTTCTCCTTTAGTCCAAAGTTTCACTGCACAGGTTGCTCAGGTGAATTCACCATTAGCTACTCAAGATAAACAGAATCAGCATCAGAAGGATGAGTTTTATCTCAATCTTGGTGTTGCTGTTAGAACCCTTCGTGAGGATATGCCTTTGATCTTTGTTAAAGACCTTAATTATGATATTTATAGGTAATTTTCTCATCTGGGTATGTAAAGTTTCTTGCTTTCGATGTAAATATATGGTTTTTAATTTGAATTGTGTTATTTAGGTGAAAAAAATTGGATTTTTAATGTTGGGTTTAGTAATTTTAAGGATGATGTAATTAATATTTATGGACCTTTTAGATGTGAATATATGGTTTTTAATTGGAATTTAGCTAATTCTGAGTAACTTGTAAGGCCTAGCTCAACTGGCAAATGCCGATATAGTTAGGCTGGACACCTTCACCTAGGTTCGAACGACTCGTAGTGGAGTGGTGTATGTGAGTTTAAAACTCATTGCCACTTTGTATGTATAAAAAAAGTTAATTTTGAGTGGAAAAAAATTGGGTTTTTATTTTCTGCAAAAAGAGTTGGAATTTTAATGTTGGGTTTGATAATTTTTCAGGGATGATGTAACATTTTTGGACCCTTTGAACACATTTACTGGTATTGAGAAGTACAAGTTGATCTTTTGGGCATTGAGGTTTCATGGTAGAATTTTGTTTCGTGAGATTGGTTTGGATGTATACAGAATATGGCAGCCTTCAGAGAATGTGATATTGATCAGGTGGAACTTGAGGGGTGTGCCTAGGGTTCCATGGGAAGCTAAAGGTGAATTTCAAGGTACTTCTCGGTATAAATTGGATCGGAACGGTAAGATTTATGAACACAAAGTTGATAATTTAGCATTCAACTTTCCACAGAAGATGATGAAACCGGTTTCGGTTTTGGATTTGGTTACTGCTTGCCCTGCTAGTCCAAATCCAACATTTTCATGGGGTCCTTTGGATTCTAGTTCATGTTCATGGATAGAATTATATCAAGCAGTTAAGCACACATTGGATGAAGAAAGACAGTTGTTACCACCAGATGGTTTGGCTACATGTTCATAG

Protein sequence

>MS.gene36105.t1

MAFPLCNLNLSLLPQSKSRFKHQTLSSSKPISQYSLFFSSSPLVQSFTAQVAQVNSPLATQDKQNQHQKDEFYLNLGVAVRTLREDMPLIFVKDLNYDIYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILFREIGLDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQKMMKPVSVLDLVTACPASPNPTFSWGPLDSSSCSWIELYQAVKHTLDEERQLLPPDGLATCS