Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28490.t1 | ADG01841.1 | 99.7 | 370 | 1 | 0 | 1 | 370 | 1 | 370 | 1.00E-200 | 709.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28490.t1 | Q94ID7 | 72.5 | 375 | 93 | 3 | 1 | 370 | 1 | 370 | 5.0e-143 | 508.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28490.t1 | D6Q1T4 | 99.7 | 370 | 1 | 0 | 1 | 370 | 1 | 370 | 7.5e-201 | 709.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene28490 | 0.804323 | 2.28E-49 | -1.69E-46 |
| MS.gene049217 | MS.gene28490 | 0.822649 | 2.12E-53 | -1.69E-46 |
| MS.gene049222 | MS.gene28490 | 0.855212 | 7.34E-62 | -1.69E-46 |
| MS.gene049232 | MS.gene28490 | 0.839409 | 1.61E-57 | -1.69E-46 |
| MS.gene049285 | MS.gene28490 | 0.814113 | 1.82E-51 | -1.69E-46 |
| MS.gene049320 | MS.gene28490 | 0.824034 | 1.00E-53 | -1.69E-46 |
| MS.gene049356 | MS.gene28490 | 0.853259 | 2.69E-61 | -1.69E-46 |
| MS.gene049369 | MS.gene28490 | 0.882679 | 8.51E-71 | -1.69E-46 |
| MS.gene049396 | MS.gene28490 | 0.867523 | 1.29E-65 | -1.69E-46 |
| MS.gene049609 | MS.gene28490 | 0.825714 | 4.03E-54 | -1.69E-46 |
| MS.gene049805 | MS.gene28490 | 0.835306 | 1.81E-56 | -1.69E-46 |
| MS.gene049828 | MS.gene28490 | 0.823162 | 1.61E-53 | -1.69E-46 |
| MS.gene049907 | MS.gene28490 | 0.829166 | 5.99E-55 | -1.69E-46 |
| MS.gene05010 | MS.gene28490 | 0.804143 | 2.49E-49 | -1.69E-46 |
| MS.gene050122 | MS.gene28490 | 0.801916 | 7.18E-49 | -1.69E-46 |
| MS.gene050216 | MS.gene28490 | 0.824255 | 8.91E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene28490 | 0.822602 | 2.17E-53 | -1.69E-46 |
| MS.gene050380 | MS.gene28490 | -0.835007 | 2.16E-56 | -1.69E-46 |
| MS.gene050499 | MS.gene28490 | 0.874862 | 4.85E-68 | -1.69E-46 |
| MS.gene050502 | MS.gene28490 | 0.850743 | 1.39E-60 | -1.69E-46 |
| MS.gene050504 | MS.gene28490 | 0.810769 | 9.78E-51 | -1.69E-46 |
| MS.gene050518 | MS.gene28490 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene050520 | MS.gene28490 | 0.844074 | 9.47E-59 | -1.69E-46 |
| MS.gene050538 | MS.gene28490 | 0.810401 | 1.17E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene28490 | 0.806211 | 9.18E-50 | -1.69E-46 |
| MS.gene050662 | MS.gene28490 | 0.818688 | 1.72E-52 | -1.69E-46 |
| MS.gene050686 | MS.gene28490 | 0.850825 | 1.32E-60 | -1.69E-46 |
| MS.gene050747 | MS.gene28490 | 0.851059 | 1.14E-60 | -1.69E-46 |
| MS.gene050772 | MS.gene28490 | 0.84852 | 5.82E-60 | -1.69E-46 |
| MS.gene05080 | MS.gene28490 | 0.802327 | 5.91E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene28490 | 0.83481 | 2.42E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene28490 | 0.839127 | 1.91E-57 | -1.69E-46 |
| MS.gene050950 | MS.gene28490 | 0.845821 | 3.20E-59 | -1.69E-46 |
| MS.gene05105 | MS.gene28490 | 0.809886 | 1.52E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene28490 | 0.852291 | 5.08E-61 | -1.69E-46 |
| MS.gene051509 | MS.gene28490 | 0.858578 | 7.48E-63 | -1.69E-46 |
| MS.gene051551 | MS.gene28490 | 0.802104 | 6.57E-49 | -1.69E-46 |
| MS.gene051666 | MS.gene28490 | 0.831675 | 1.46E-55 | -1.69E-46 |
| MS.gene051692 | MS.gene28490 | 0.813582 | 2.38E-51 | -1.69E-46 |
| MS.gene051694 | MS.gene28490 | 0.812692 | 3.73E-51 | -1.69E-46 |
| MS.gene051734 | MS.gene28490 | 0.804256 | 2.36E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene28490 | 0.816442 | 5.52E-52 | -1.69E-46 |
| MS.gene051874 | MS.gene28490 | 0.80674 | 7.10E-50 | -1.69E-46 |
| MS.gene052164 | MS.gene28490 | 0.82959 | 4.73E-55 | -1.69E-46 |
| MS.gene052414 | MS.gene28490 | 0.813215 | 2.87E-51 | -1.69E-46 |
| MS.gene052421 | MS.gene28490 | 0.839747 | 1.32E-57 | -1.69E-46 |
| MS.gene052486 | MS.gene28490 | 0.801903 | 7.23E-49 | -1.69E-46 |
| MS.gene052503 | MS.gene28490 | 0.81647 | 5.44E-52 | -1.69E-46 |
| MS.gene052615 | MS.gene28490 | 0.819648 | 1.04E-52 | -1.69E-46 |
| MS.gene052632 | MS.gene28490 | 0.836578 | 8.62E-57 | -1.69E-46 |
| MS.gene052823 | MS.gene28490 | 0.81677 | 4.66E-52 | -1.69E-46 |
| MS.gene052825 | MS.gene28490 | 0.809208 | 2.12E-50 | -1.69E-46 |
| MS.gene052916 | MS.gene28490 | 0.804858 | 1.76E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene28490 | 0.803399 | 3.55E-49 | -1.69E-46 |
| MS.gene053018 | MS.gene28490 | 0.848931 | 4.47E-60 | -1.69E-46 |
| MS.gene053103 | MS.gene28490 | 0.801027 | 1.09E-48 | -1.69E-46 |
| MS.gene053104 | MS.gene28490 | 0.815961 | 7.07E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene28490 | 0.810968 | 8.85E-51 | -1.69E-46 |
| MS.gene053213 | MS.gene28490 | 0.844129 | 9.15E-59 | -1.69E-46 |
| MS.gene053286 | MS.gene28490 | 0.842788 | 2.09E-58 | -1.69E-46 |
| MS.gene053436 | MS.gene28490 | 0.849438 | 3.23E-60 | -1.69E-46 |
| MS.gene053449 | MS.gene28490 | 0.838081 | 3.55E-57 | -1.69E-46 |
| MS.gene053505 | MS.gene28490 | 0.861994 | 6.93E-64 | -1.69E-46 |
| MS.gene053574 | MS.gene28490 | 0.873282 | 1.66E-67 | -1.69E-46 |
| MS.gene053626 | MS.gene28490 | 0.874563 | 6.12E-68 | -1.69E-46 |
| MS.gene053632 | MS.gene28490 | 0.826428 | 2.73E-54 | -1.69E-46 |
| MS.gene053633 | MS.gene28490 | 0.801477 | 8.84E-49 | -1.69E-46 |
| MS.gene053813 | MS.gene28490 | 0.85553 | 5.93E-62 | -1.69E-46 |
| MS.gene053824 | MS.gene28490 | 0.818037 | 2.42E-52 | -1.69E-46 |
| MS.gene053912 | MS.gene28490 | 0.842594 | 2.35E-58 | -1.69E-46 |
| MS.gene053916 | MS.gene28490 | 0.858617 | 7.29E-63 | -1.69E-46 |
| MS.gene054003 | MS.gene28490 | 0.847118 | 1.42E-59 | -1.69E-46 |
| MS.gene054408 | MS.gene28490 | 0.827737 | 1.33E-54 | -1.69E-46 |
| MS.gene054486 | MS.gene28490 | 0.857073 | 2.09E-62 | -1.69E-46 |
| MS.gene054595 | MS.gene28490 | 0.855195 | 7.43E-62 | -1.69E-46 |
| MS.gene054600 | MS.gene28490 | 0.806508 | 7.95E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene28490 | 0.818856 | 1.58E-52 | -1.69E-46 |
| MS.gene054706 | MS.gene28490 | -0.817192 | 3.75E-52 | -1.69E-46 |
| MS.gene05494 | MS.gene28490 | 0.833102 | 6.47E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene28490 | 0.884057 | 2.65E-71 | -1.69E-46 |
| MS.gene055212 | MS.gene28490 | 0.810789 | 9.68E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene28490 | 0.825566 | 4.37E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene28490 | 0.803235 | 3.84E-49 | -1.69E-46 |
| MS.gene055486 | MS.gene28490 | 0.813265 | 2.79E-51 | -1.69E-46 |
| MS.gene055502 | MS.gene28490 | 0.816026 | 6.84E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene28490 | 0.820883 | 5.42E-53 | -1.69E-46 |
| MS.gene055629 | MS.gene28490 | 0.816378 | 5.70E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene28490 | 0.829684 | 4.49E-55 | -1.69E-46 |
| MS.gene055750 | MS.gene28490 | 0.820333 | 7.25E-53 | -1.69E-46 |
| MS.gene055909 | MS.gene28490 | 0.874271 | 7.69E-68 | -1.69E-46 |
| MS.gene055911 | MS.gene28490 | 0.875147 | 3.87E-68 | -1.69E-46 |
| MS.gene05592 | MS.gene28490 | 0.83905 | 2.00E-57 | -1.69E-46 |
| MS.gene055941 | MS.gene28490 | 0.832544 | 8.90E-56 | -1.69E-46 |
| MS.gene05601 | MS.gene28490 | 0.813314 | 2.72E-51 | -1.69E-46 |
| MS.gene056166 | MS.gene28490 | 0.836524 | 8.90E-57 | -1.69E-46 |
| MS.gene056374 | MS.gene28490 | 0.815279 | 1.00E-51 | -1.69E-46 |
| MS.gene056387 | MS.gene28490 | 0.819749 | 9.86E-53 | -1.69E-46 |
| MS.gene056422 | MS.gene28490 | 0.885076 | 1.11E-71 | -1.69E-46 |
| MS.gene056446 | MS.gene28490 | 0.814306 | 1.65E-51 | -1.69E-46 |
| MS.gene056448 | MS.gene28490 | 0.855565 | 5.79E-62 | -1.69E-46 |
| MS.gene056487 | MS.gene28490 | 0.86173 | 8.35E-64 | -1.69E-46 |
| MS.gene056504 | MS.gene28490 | 0.82375 | 1.17E-53 | -1.69E-46 |
| MS.gene056510 | MS.gene28490 | 0.807304 | 5.40E-50 | -1.69E-46 |
| MS.gene056511 | MS.gene28490 | 0.854282 | 1.37E-61 | -1.69E-46 |
| MS.gene056549 | MS.gene28490 | 0.808763 | 2.64E-50 | -1.69E-46 |
| MS.gene05664 | MS.gene28490 | 0.834724 | 2.54E-56 | -1.69E-46 |
| MS.gene056657 | MS.gene28490 | 0.844068 | 9.51E-59 | -1.69E-46 |
| MS.gene05666 | MS.gene28490 | 0.857928 | 1.17E-62 | -1.69E-46 |
| MS.gene056942 | MS.gene28490 | 0.840776 | 7.09E-58 | -1.69E-46 |
| MS.gene056947 | MS.gene28490 | 0.809351 | 1.98E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene28490 | 0.828493 | 8.72E-55 | -1.69E-46 |
| MS.gene057086 | MS.gene28490 | 0.849884 | 2.43E-60 | -1.69E-46 |
| MS.gene057146 | MS.gene28490 | 0.816063 | 6.71E-52 | -1.69E-46 |
| MS.gene057285 | MS.gene28490 | 0.811048 | 8.50E-51 | -1.69E-46 |
| MS.gene05735 | MS.gene28490 | 0.810676 | 1.02E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene28490 | 0.83448 | 2.93E-56 | -1.69E-46 |
| MS.gene057734 | MS.gene28490 | 0.847693 | 9.84E-60 | -1.69E-46 |
| MS.gene05787 | MS.gene28490 | 0.858625 | 7.24E-63 | -1.69E-46 |
| MS.gene057926 | MS.gene28490 | 0.867194 | 1.64E-65 | -1.69E-46 |
| MS.gene058019 | MS.gene28490 | 0.806139 | 9.51E-50 | -1.69E-46 |
| MS.gene058075 | MS.gene28490 | 0.890318 | 1.10E-73 | -1.69E-46 |
| MS.gene058120 | MS.gene28490 | 0.826015 | 3.42E-54 | -1.69E-46 |
| MS.gene058130 | MS.gene28490 | 0.840715 | 7.36E-58 | -1.69E-46 |
| MS.gene058157 | MS.gene28490 | 0.834388 | 3.09E-56 | -1.69E-46 |
| MS.gene058323 | MS.gene28490 | 0.810656 | 1.03E-50 | -1.69E-46 |
| MS.gene058376 | MS.gene28490 | 0.813933 | 1.99E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene28490 | 0.819222 | 1.30E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene28490 | 0.810633 | 1.05E-50 | -1.69E-46 |
| MS.gene058459 | MS.gene28490 | 0.851701 | 7.47E-61 | -1.69E-46 |
| MS.gene058773 | MS.gene28490 | 0.800232 | 1.59E-48 | -1.69E-46 |
| MS.gene058788 | MS.gene28490 | 0.802847 | 4.62E-49 | -1.69E-46 |
| MS.gene058790 | MS.gene28490 | 0.8347 | 2.58E-56 | -1.69E-46 |
| MS.gene058928 | MS.gene28490 | 0.806208 | 9.20E-50 | -1.69E-46 |
| MS.gene058948 | MS.gene28490 | 0.833282 | 5.83E-56 | -1.69E-46 |
| MS.gene059037 | MS.gene28490 | -0.811718 | 6.09E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene28490 | 0.842964 | 1.87E-58 | -1.69E-46 |
| MS.gene059305 | MS.gene28490 | 0.847487 | 1.12E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene28490 | 0.837073 | 6.44E-57 | -1.69E-46 |
| MS.gene059367 | MS.gene28490 | 0.892429 | 1.60E-74 | -1.69E-46 |
| MS.gene059368 | MS.gene28490 | 0.889461 | 2.37E-73 | -1.69E-46 |
| MS.gene059517 | MS.gene28490 | 0.803056 | 4.18E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene28490 | 0.819959 | 8.83E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene28490 | 0.849 | 4.28E-60 | -1.69E-46 |
| MS.gene059680 | MS.gene28490 | 0.829013 | 6.53E-55 | -1.69E-46 |
| MS.gene059711 | MS.gene28490 | 0.877709 | 5.05E-69 | -1.69E-46 |
| MS.gene059718 | MS.gene28490 | 0.827397 | 1.60E-54 | -1.69E-46 |
| MS.gene059801 | MS.gene28490 | 0.806248 | 9.02E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene28490 | 0.845038 | 5.21E-59 | -1.69E-46 |
| MS.gene059865 | MS.gene28490 | 0.819025 | 1.44E-52 | -1.69E-46 |
| MS.gene059870 | MS.gene28490 | 0.810387 | 1.18E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene28490 | 0.809532 | 1.81E-50 | -1.69E-46 |
| MS.gene060356 | MS.gene28490 | 0.805108 | 1.57E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene28490 | 0.82079 | 5.69E-53 | -1.69E-46 |
| MS.gene060482 | MS.gene28490 | 0.883122 | 5.86E-71 | -1.69E-46 |
| MS.gene060778 | MS.gene28490 | 0.840771 | 7.11E-58 | -1.69E-46 |
| MS.gene06077 | MS.gene28490 | 0.80105 | 1.08E-48 | -1.69E-46 |
| MS.gene060804 | MS.gene28490 | 0.865199 | 7.02E-65 | -1.69E-46 |
| MS.gene060818 | MS.gene28490 | 0.81953 | 1.11E-52 | -1.69E-46 |
| MS.gene060835 | MS.gene28490 | 0.833386 | 5.50E-56 | -1.69E-46 |
| MS.gene060837 | MS.gene28490 | 0.872542 | 2.94E-67 | -1.69E-46 |
| MS.gene06097 | MS.gene28490 | 0.877484 | 6.06E-69 | -1.69E-46 |
| MS.gene061041 | MS.gene28490 | 0.880764 | 4.20E-70 | -1.69E-46 |
| MS.gene061134 | MS.gene28490 | 0.861923 | 7.29E-64 | -1.69E-46 |
| MS.gene061200 | MS.gene28490 | 0.832447 | 9.41E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28490.t1 | MTR_8g078070 | 97.587 | 373 | 5 | 2 | 1 | 370 | 1 | 372 | 0.0 | 737 |
| MS.gene28490.t1 | MTR_5g019460 | 73.684 | 323 | 85 | 0 | 48 | 370 | 49 | 371 | 1.94e-176 | 495 |
| MS.gene28490.t1 | MTR_2g072470 | 37.751 | 249 | 135 | 7 | 90 | 331 | 113 | 348 | 3.31e-36 | 136 |
| MS.gene28490.t1 | MTR_5g092910 | 32.039 | 206 | 131 | 2 | 90 | 291 | 54 | 254 | 2.29e-24 | 101 |
| MS.gene28490.t1 | MTR_4g095055 | 31.984 | 247 | 137 | 7 | 105 | 331 | 103 | 338 | 9.62e-21 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene28490.t1 | AT4G36810 | 70.856 | 374 | 102 | 5 | 1 | 370 | 1 | 371 | 0.0 | 517 |
| MS.gene28490.t1 | AT2G18620 | 72.647 | 340 | 87 | 3 | 36 | 370 | 9 | 347 | 1.12e-174 | 489 |
| MS.gene28490.t1 | AT2G18640 | 69.257 | 296 | 87 | 2 | 77 | 370 | 79 | 372 | 9.91e-150 | 427 |
| MS.gene28490.t1 | AT2G23800 | 68.562 | 299 | 90 | 2 | 74 | 370 | 80 | 376 | 4.98e-148 | 423 |
| MS.gene28490.t1 | AT3G20160 | 66.221 | 299 | 94 | 4 | 77 | 370 | 48 | 344 | 2.84e-142 | 407 |
| MS.gene28490.t1 | AT3G14550 | 67.257 | 339 | 100 | 5 | 42 | 370 | 23 | 360 | 3.60e-142 | 408 |
| MS.gene28490.t1 | AT3G14550 | 67.257 | 339 | 100 | 5 | 42 | 370 | 23 | 360 | 3.60e-142 | 408 |
| MS.gene28490.t1 | AT3G14550 | 67.257 | 339 | 100 | 5 | 42 | 370 | 23 | 360 | 3.60e-142 | 408 |
| MS.gene28490.t1 | AT3G14530 | 65.782 | 339 | 105 | 5 | 42 | 370 | 23 | 360 | 4.16e-140 | 402 |
| MS.gene28490.t1 | AT3G29430 | 70.492 | 305 | 87 | 2 | 69 | 370 | 53 | 357 | 2.17e-138 | 398 |
| MS.gene28490.t1 | AT3G14510 | 72.563 | 277 | 73 | 2 | 97 | 370 | 8 | 284 | 2.27e-138 | 395 |
| MS.gene28490.t1 | AT3G32040 | 68.852 | 305 | 92 | 2 | 69 | 370 | 56 | 360 | 2.12e-135 | 390 |
| MS.gene28490.t1 | AT3G20160 | 60.473 | 296 | 88 | 4 | 77 | 370 | 48 | 316 | 1.88e-123 | 358 |
| MS.gene28490.t1 | AT1G49530 | 59.344 | 305 | 111 | 4 | 79 | 370 | 32 | 336 | 4.48e-123 | 358 |
| MS.gene28490.t1 | AT4G38460 | 46.250 | 240 | 120 | 3 | 77 | 313 | 45 | 278 | 4.97e-72 | 228 |
| MS.gene28490.t1 | AT1G78510 | 36.404 | 228 | 128 | 5 | 116 | 337 | 84 | 300 | 2.69e-33 | 127 |
| MS.gene28490.t1 | AT1G17050 | 31.045 | 335 | 208 | 10 | 14 | 337 | 28 | 350 | 8.17e-33 | 127 |
| MS.gene28490.t1 | AT1G78510 | 36.404 | 228 | 128 | 5 | 116 | 337 | 123 | 339 | 8.39e-33 | 127 |
| MS.gene28490.t1 | AT2G34630 | 31.443 | 194 | 122 | 3 | 148 | 341 | 171 | 353 | 1.31e-18 | 87.0 |
| MS.gene28490.t1 | AT2G34630 | 31.313 | 198 | 122 | 4 | 148 | 342 | 70 | 256 | 1.89e-18 | 85.5 |
Find 75 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATATCGGTTTATTGTTTC+AGG | 0.214767 | 8.4:-28524398 | MS.gene28490:CDS |
| TGGCTAATTACATTGCTTAT+AGG | 0.225968 | 8.4:-28524174 | MS.gene28490:CDS |
| GATGCACAAGAACAACTTTC+TGG | 0.227841 | 8.4:-28524232 | MS.gene28490:CDS |
| TTAGAAGGTGCTGTTGTTCT+TGG | 0.252595 | 8.4:-28524478 | MS.gene28490:CDS |
| TGAAACTGAAATGGGTATTT+TGG | 0.254823 | 8.4:+28525146 | None:intergenic |
| CCGAAGACTTTGTGGTTTGT+AGG | 0.273097 | 8.4:+28524739 | None:intergenic |
| AACTTGATTGAACATGAAAT+TGG | 0.287751 | 8.4:+28525221 | None:intergenic |
| GTTGTTCTTGGTGCAATATT+AGG | 0.288632 | 8.4:-28524466 | MS.gene28490:CDS |
| CGAAAATTCGCGAGATATAT+CGG | 0.296068 | 8.4:-28524412 | MS.gene28490:CDS |
| ATCTGCTCCTTTGATTGCTT+TGG | 0.315020 | 8.4:-28524194 | MS.gene28490:CDS |
| AAAACTGCGGCTTTGTTAGA+AGG | 0.342543 | 8.4:-28524493 | MS.gene28490:CDS |
| CGTCTTCTCCGAAGACTTTG+TGG | 0.343711 | 8.4:+28524731 | None:intergenic |
| AAAATAGAAAGATGGGATTC+TGG | 0.344376 | 8.4:+28525170 | None:intergenic |
| TAATGAAACGGCGTCGTCTA+AGG | 0.368203 | 8.4:+28524978 | None:intergenic |
| GGCTCGGAAGGACTCGTTGC+TGG | 0.376904 | 8.4:-28524589 | MS.gene28490:CDS |
| GAAAGATTTGGTAGCTGATA+AGG | 0.381248 | 8.4:-28524317 | MS.gene28490:CDS |
| TAGGATTTGAAATTGAAGAT+TGG | 0.396930 | 8.4:+28525033 | None:intergenic |
| AGGATTTGAAATTGAAGATT+GGG | 0.398523 | 8.4:+28525034 | None:intergenic |
| CCACGACGACCTTCCTTGTA+TGG | 0.400090 | 8.4:-28524788 | MS.gene28490:CDS |
| TAAAACTGCTGGGAAAGATT+TGG | 0.402177 | 8.4:-28524329 | MS.gene28490:CDS |
| TTTAATGGGTGAAACTGAAA+TGG | 0.414231 | 8.4:+28525137 | None:intergenic |
| CAGGCGGCCGGCATCGCCAT+CGG | 0.414541 | 8.4:+28524844 | None:intergenic |
| GGCCGGCATCGCCATCGGTT+CGG | 0.427454 | 8.4:+28524849 | None:intergenic |
| GAAAGATGGGATTCTGGATC+TGG | 0.430542 | 8.4:+28525176 | None:intergenic |
| ACGAAATCTTCACAGGAGTT+AGG | 0.431856 | 8.4:-28524352 | MS.gene28490:CDS |
| ATTTCAACGGCGCAGGCGGC+CGG | 0.436594 | 8.4:+28524832 | None:intergenic |
| ATGCACAAGAACAACTTTCT+GGG | 0.437672 | 8.4:-28524231 | MS.gene28490:CDS |
| GCGAGAATTGTCCGTGCAAT+CGG | 0.446559 | 8.4:-28524631 | MS.gene28490:CDS |
| TAACGGTTCGCGTAATGAAA+CGG | 0.452982 | 8.4:+28524966 | None:intergenic |
| AACCGAACCGATGGCGATGC+CGG | 0.457738 | 8.4:-28524851 | MS.gene28490:CDS |
| TCGCCGGCGGAAAACGTGTC+AGG | 0.458070 | 8.4:-28524912 | MS.gene28490:CDS |
| CCTACAAACCACAAAGTCTT+CGG | 0.467203 | 8.4:-28524739 | MS.gene28490:CDS |
| GGTGAATTAGCAAAATCAAT+TGG | 0.470164 | 8.4:-28524610 | MS.gene28490:CDS |
| CAGGAGTTAGGTAAAACTGC+TGG | 0.471173 | 8.4:-28524340 | MS.gene28490:CDS |
| CGCCGGAGAAACACCGGCGG+TGG | 0.473224 | 8.4:+28524651 | None:intergenic |
| CTCCACCGCCGGTGTTTCTC+CGG | 0.475973 | 8.4:-28524653 | MS.gene28490:CDS |
| AAAAGCGAGAAGAGCGTCAC+CGG | 0.482767 | 8.4:+28524696 | None:intergenic |
| GTATGGATAACGACGATCTC+CGG | 0.512700 | 8.4:-28524771 | MS.gene28490:CDS |
| TTGCTAATTCACCGATTGCA+CGG | 0.521598 | 8.4:+28524620 | None:intergenic |
| CAGGCGGCTAAACAGAGAAC+TGG | 0.522019 | 8.4:+28524889 | None:intergenic |
| GTGGATCATTTCAACGGCGC+AGG | 0.524802 | 8.4:+28524825 | None:intergenic |
| GGTTCCGCCGACGAGTTCGC+AGG | 0.527687 | 8.4:+28524870 | None:intergenic |
| TCAGAAGGTTTATCCGATGT+TGG | 0.530197 | 8.4:-28524547 | MS.gene28490:CDS |
| GCAAAATCAATTGGCTCGGA+AGG | 0.538303 | 8.4:-28524601 | MS.gene28490:CDS |
| GGTTTGTAGGTTTACCTCGC+CGG | 0.539958 | 8.4:+28524752 | None:intergenic |
| TTAATGGGTGAAACTGAAAT+GGG | 0.544577 | 8.4:+28525138 | None:intergenic |
| GTTGTTGATATCAACTCAGA+AGG | 0.546305 | 8.4:-28524562 | MS.gene28490:CDS |
| GATCATTTCAACGGCGCAGG+CGG | 0.547898 | 8.4:+28524828 | None:intergenic |
| TGATGTGACGAAATCTTCAC+AGG | 0.548692 | 8.4:-28524359 | MS.gene28490:CDS |
| CATATCGCTGTCTCCACCGC+CGG | 0.579244 | 8.4:-28524664 | MS.gene28490:CDS |
| AATTCTCGCCGGAGAAACAC+CGG | 0.583236 | 8.4:+28524645 | None:intergenic |
| ATTAGCAAAATCAATTGGCT+CGG | 0.586837 | 8.4:-28524605 | MS.gene28490:CDS |
| GAAGACGTTGCTGTTCTCGC+CGG | 0.587256 | 8.4:-28524715 | MS.gene28490:CDS |
| AAAGTTGTTCTTGTGCATCA+CGG | 0.594174 | 8.4:+28524236 | None:intergenic |
| AGGAGTTAGGTAAAACTGCT+GGG | 0.596113 | 8.4:-28524339 | MS.gene28490:CDS |
| CGTCGGCGGAACCGAACCGA+TGG | 0.596354 | 8.4:-28524860 | MS.gene28490:CDS |
| CATCGTGTGGATCATTTCAA+CGG | 0.597412 | 8.4:+28524819 | None:intergenic |
| AGATCGTCGTTATCCATACA+AGG | 0.598288 | 8.4:+28524775 | None:intergenic |
| ATGCGTTACTCTCTCCTCGC+CGG | 0.606596 | 8.4:-28524928 | MS.gene28490:CDS |
| TAATTAGCCAAAGCAATCAA+AGG | 0.617462 | 8.4:+28524187 | None:intergenic |
| GCCGCCTGCGAACTCGTCGG+CGG | 0.623082 | 8.4:-28524874 | MS.gene28490:CDS |
| GTTCTTGGTGCAATATTAGG+TGG | 0.624578 | 8.4:-28524463 | MS.gene28490:CDS |
| CTTGGTGCAATATTAGGTGG+TGG | 0.625172 | 8.4:-28524460 | MS.gene28490:CDS |
| CGTCGTTATCCATACAAGGA+AGG | 0.629409 | 8.4:+28524779 | None:intergenic |
| CATTCATCTGCATAAAACTG+CGG | 0.633100 | 8.4:-28524506 | MS.gene28490:CDS |
| TCCGCCGACGAGTTCGCAGG+CGG | 0.634828 | 8.4:+28524873 | None:intergenic |
| CGTTACTCTCTCCTCGCCGG+CGG | 0.638430 | 8.4:-28524925 | MS.gene28490:CDS |
| TTAGCCGCCTGCGAACTCGT+CGG | 0.642109 | 8.4:-28524877 | MS.gene28490:CDS |
| CCATACAAGGAAGGTCGTCG+TGG | 0.653424 | 8.4:+28524788 | None:intergenic |
| AAGCTGTTCGAGTCCAACAT+CGG | 0.661724 | 8.4:+28524534 | None:intergenic |
| CGTGGATTAGAGACATCGTG+TGG | 0.678617 | 8.4:+28524806 | None:intergenic |
| ATTGCACGGACAATTCTCGC+CGG | 0.689391 | 8.4:+28524634 | None:intergenic |
| GATAACGACGATCTCCGGCG+AGG | 0.695961 | 8.4:-28524766 | MS.gene28490:CDS |
| GTGCTGTGAATCTTAACACA+TGG | 0.731215 | 8.4:-28525251 | MS.gene28490:CDS |
| TCTCGCCGGAGAAACACCGG+CGG | 0.762110 | 8.4:+28524648 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTGGTAAAATAGAAAGA+TGG | + | chr8.4:28524261-28524280 | None:intergenic | 20.0% |
| !!! | TTTTGGTAAAATAGAAAGAT+GGG | + | chr8.4:28524260-28524279 | None:intergenic | 20.0% |
| ! | AACTTGATTGAACATGAAAT+TGG | + | chr8.4:28524202-28524221 | None:intergenic | 25.0% |
| ! | GAAACAGAAAAAGAAGAATT+GGG | + | chr8.4:28524321-28524340 | None:intergenic | 25.0% |
| ! | TGAAACAGAAAAAGAAGAAT+TGG | + | chr8.4:28524322-28524341 | None:intergenic | 25.0% |
| !! | AGGATTTGAAATTGAAGATT+GGG | + | chr8.4:28524389-28524408 | None:intergenic | 25.0% |
| !! | TAGGATTTGAAATTGAAGAT+TGG | + | chr8.4:28524390-28524409 | None:intergenic | 25.0% |
| !! | TTTTCAATTCCTAAAAGCTT+AGG | + | chr8.4:28525137-28525156 | None:intergenic | 25.0% |
| !!! | ATATATCGGTTTATTGTTTC+AGG | - | chr8.4:28525022-28525041 | MS.gene28490:CDS | 25.0% |
| AAAATAGAAAGATGGGATTC+TGG | + | chr8.4:28524253-28524272 | None:intergenic | 30.0% | |
| ACATGATAGAAAAAGCAACT+AGG | - | chr8.4:28524409-28524428 | MS.gene28490:CDS | 30.0% | |
| ATTAGCAAAATCAATTGGCT+CGG | - | chr8.4:28524815-28524834 | MS.gene28490:CDS | 30.0% | |
| CATGATAGAAAAAGCAACTA+GGG | - | chr8.4:28524410-28524429 | MS.gene28490:CDS | 30.0% | |
| GGTGAATTAGCAAAATCAAT+TGG | - | chr8.4:28524810-28524829 | MS.gene28490:CDS | 30.0% | |
| TAATTAGCCAAAGCAATCAA+AGG | + | chr8.4:28525236-28525255 | None:intergenic | 30.0% | |
| TGGCTAATTACATTGCTTAT+AGG | - | chr8.4:28525246-28525265 | MS.gene28490:CDS | 30.0% | |
| ! | GTTACTTATCCTAAGCTTTT+AGG | - | chr8.4:28525125-28525144 | MS.gene28490:CDS | 30.0% |
| ! | TGAAACTGAAATGGGTATTT+TGG | + | chr8.4:28524277-28524296 | None:intergenic | 30.0% |
| ! | TTAATGGGTGAAACTGAAAT+GGG | + | chr8.4:28524285-28524304 | None:intergenic | 30.0% |
| ! | TTTAATGGGTGAAACTGAAA+TGG | + | chr8.4:28524286-28524305 | None:intergenic | 30.0% |
| ! | TTTTGTTCTTCTGCTTCTAT+TGG | + | chr8.4:28524366-28524385 | None:intergenic | 30.0% |
| !!! | AGTTGCTTTTTCTATCATGT+AGG | + | chr8.4:28524409-28524428 | None:intergenic | 30.0% |
| !!! | GGTTTTGATGATGGTTTAAT+GGG | + | chr8.4:28524300-28524319 | None:intergenic | 30.0% |
| AAAGTTGTTCTTGTGCATCA+CGG | + | chr8.4:28525187-28525206 | None:intergenic | 35.0% | |
| ATGCACAAGAACAACTTTCT+GGG | - | chr8.4:28525189-28525208 | MS.gene28490:CDS | 35.0% | |
| CATTCATCTGCATAAAACTG+CGG | - | chr8.4:28524914-28524933 | MS.gene28490:CDS | 35.0% | |
| CGAAAATTCGCGAGATATAT+CGG | - | chr8.4:28525008-28525027 | MS.gene28490:CDS | 35.0% | |
| GTTGTTGATATCAACTCAGA+AGG | - | chr8.4:28524858-28524877 | MS.gene28490:CDS | 35.0% | |
| TAAAACTGCTGGGAAAGATT+TGG | - | chr8.4:28525091-28525110 | MS.gene28490:CDS | 35.0% | |
| ! | ATCGCTTCGTGAACTTTTAA+CGG | + | chr8.4:28524474-28524493 | None:intergenic | 35.0% |
| ! | GAAAGATTTGGTAGCTGATA+AGG | - | chr8.4:28525103-28525122 | MS.gene28490:CDS | 35.0% |
| ! | GTTGTTCTTGGTGCAATATT+AGG | - | chr8.4:28524954-28524973 | MS.gene28490:CDS | 35.0% |
| !! | GAAGAATTGGGTTTTGATGA+TGG | + | chr8.4:28524309-28524328 | None:intergenic | 35.0% |
| !!! | GGGTTTTGATGATGGTTTAA+TGG | + | chr8.4:28524301-28524320 | None:intergenic | 35.0% |
| ACGAAATCTTCACAGGAGTT+AGG | - | chr8.4:28525068-28525087 | MS.gene28490:CDS | 40.0% | |
| AGATCGTCGTTATCCATACA+AGG | + | chr8.4:28524648-28524667 | None:intergenic | 40.0% | |
| AGGAGTTAGGTAAAACTGCT+GGG | - | chr8.4:28525081-28525100 | MS.gene28490:CDS | 40.0% | |
| ATCTGCTCCTTTGATTGCTT+TGG | - | chr8.4:28525226-28525245 | MS.gene28490:CDS | 40.0% | |
| CATCGTGTGGATCATTTCAA+CGG | + | chr8.4:28524604-28524623 | None:intergenic | 40.0% | |
| CCTACAAACCACAAAGTCTT+CGG | - | chr8.4:28524681-28524700 | MS.gene28490:CDS | 40.0% | |
| GATGCACAAGAACAACTTTC+TGG | - | chr8.4:28525188-28525207 | MS.gene28490:CDS | 40.0% | |
| GTGCTGTGAATCTTAACACA+TGG | - | chr8.4:28524169-28524188 | MS.gene28490:CDS | 40.0% | |
| TAACGGTTCGCGTAATGAAA+CGG | + | chr8.4:28524457-28524476 | None:intergenic | 40.0% | |
| TCAGAAGGTTTATCCGATGT+TGG | - | chr8.4:28524873-28524892 | MS.gene28490:CDS | 40.0% | |
| TGATGTGACGAAATCTTCAC+AGG | - | chr8.4:28525061-28525080 | MS.gene28490:CDS | 40.0% | |
| ! | GTTCTTGGTGCAATATTAGG+TGG | - | chr8.4:28524957-28524976 | MS.gene28490:CDS | 40.0% |
| ! | TTGCTAATTCACCGATTGCA+CGG | + | chr8.4:28524803-28524822 | None:intergenic | 40.0% |
| !! | AAAACTGCGGCTTTGTTAGA+AGG | - | chr8.4:28524927-28524946 | MS.gene28490:CDS | 40.0% |
| !! | TTAGAAGGTGCTGTTGTTCT+TGG | - | chr8.4:28524942-28524961 | MS.gene28490:CDS | 40.0% |
| AAGCTGTTCGAGTCCAACAT+CGG | + | chr8.4:28524889-28524908 | None:intergenic | 45.0% | |
| CAGGAGTTAGGTAAAACTGC+TGG | - | chr8.4:28525080-28525099 | MS.gene28490:CDS | 45.0% | |
| CGTCGTTATCCATACAAGGA+AGG | + | chr8.4:28524644-28524663 | None:intergenic | 45.0% | |
| GAAAGATGGGATTCTGGATC+TGG | + | chr8.4:28524247-28524266 | None:intergenic | 45.0% | |
| GTATGGATAACGACGATCTC+CGG | - | chr8.4:28524649-28524668 | MS.gene28490:CDS | 45.0% | |
| TAATGAAACGGCGTCGTCTA+AGG | + | chr8.4:28524445-28524464 | None:intergenic | 45.0% | |
| ! | CCGAAGACTTTGTGGTTTGT+AGG | + | chr8.4:28524684-28524703 | None:intergenic | 45.0% |
| ! | CTTGGTGCAATATTAGGTGG+TGG | - | chr8.4:28524960-28524979 | MS.gene28490:CDS | 45.0% |
| !! | GCAAAATCAATTGGCTCGGA+AGG | - | chr8.4:28524819-28524838 | MS.gene28490:CDS | 45.0% |
| AATTCTCGCCGGAGAAACAC+CGG | + | chr8.4:28524778-28524797 | None:intergenic | 50.0% | |
| ATTGCACGGACAATTCTCGC+CGG | + | chr8.4:28524789-28524808 | None:intergenic | 50.0% | |
| CGTCTTCTCCGAAGACTTTG+TGG | + | chr8.4:28524692-28524711 | None:intergenic | 50.0% | |
| GCGAGAATTGTCCGTGCAAT+CGG | - | chr8.4:28524789-28524808 | MS.gene28490:CDS | 50.0% | |
| GGTTTGTAGGTTTACCTCGC+CGG | + | chr8.4:28524671-28524690 | None:intergenic | 50.0% | |
| !! | AAAAGCGAGAAGAGCGTCAC+CGG | + | chr8.4:28524727-28524746 | None:intergenic | 50.0% |
| !! | CGTGGATTAGAGACATCGTG+TGG | + | chr8.4:28524617-28524636 | None:intergenic | 50.0% |
| ATGCGTTACTCTCTCCTCGC+CGG | - | chr8.4:28524492-28524511 | MS.gene28490:CDS | 55.0% | |
| CAGGCGGCTAAACAGAGAAC+TGG | + | chr8.4:28524534-28524553 | None:intergenic | 55.0% | |
| CCACGACGACCTTCCTTGTA+TGG | - | chr8.4:28524632-28524651 | MS.gene28490:CDS | 55.0% | |
| CCATACAAGGAAGGTCGTCG+TGG | + | chr8.4:28524635-28524654 | None:intergenic | 55.0% | |
| GATCATTTCAACGGCGCAGG+CGG | + | chr8.4:28524595-28524614 | None:intergenic | 55.0% | |
| GTGGATCATTTCAACGGCGC+AGG | + | chr8.4:28524598-28524617 | None:intergenic | 55.0% | |
| ! | GAAGACGTTGCTGTTCTCGC+CGG | - | chr8.4:28524705-28524724 | MS.gene28490:CDS | 55.0% |
| AACCGAACCGATGGCGATGC+CGG | - | chr8.4:28524569-28524588 | MS.gene28490:CDS | 60.0% | |
| CATATCGCTGTCTCCACCGC+CGG | - | chr8.4:28524756-28524775 | MS.gene28490:CDS | 60.0% | |
| GATAACGACGATCTCCGGCG+AGG | - | chr8.4:28524654-28524673 | MS.gene28490:CDS | 60.0% | |
| TTAGCCGCCTGCGAACTCGT+CGG | - | chr8.4:28524543-28524562 | MS.gene28490:CDS | 60.0% | |
| ! | TGGCCTGACACGTTTTCCGC+CGG | + | chr8.4:28524514-28524533 | None:intergenic | 60.0% |
| ATTTCAACGGCGCAGGCGGC+CGG | + | chr8.4:28524591-28524610 | None:intergenic | 65.0% | |
| CGTTACTCTCTCCTCGCCGG+CGG | - | chr8.4:28524495-28524514 | MS.gene28490:CDS | 65.0% | |
| CTCCACCGCCGGTGTTTCTC+CGG | - | chr8.4:28524767-28524786 | MS.gene28490:CDS | 65.0% | |
| GGCTCGGAAGGACTCGTTGC+TGG | - | chr8.4:28524831-28524850 | MS.gene28490:CDS | 65.0% | |
| TCGCCGGCGGAAAACGTGTC+AGG | - | chr8.4:28524508-28524527 | MS.gene28490:CDS | 65.0% | |
| TCTCGCCGGAGAAACACCGG+CGG | + | chr8.4:28524775-28524794 | None:intergenic | 65.0% | |
| ! | TGACACGTTTTCCGCCGGCG+AGG | + | chr8.4:28524509-28524528 | None:intergenic | 65.0% |
| CGTCGGCGGAACCGAACCGA+TGG | - | chr8.4:28524560-28524579 | MS.gene28490:CDS | 70.0% | |
| GGTTCCGCCGACGAGTTCGC+AGG | + | chr8.4:28524553-28524572 | None:intergenic | 70.0% | |
| TCCGCCGACGAGTTCGCAGG+CGG | + | chr8.4:28524550-28524569 | None:intergenic | 70.0% | |
| ! | GGCCGGCATCGCCATCGGTT+CGG | + | chr8.4:28524574-28524593 | None:intergenic | 70.0% |
| CAGGCGGCCGGCATCGCCAT+CGG | + | chr8.4:28524579-28524598 | None:intergenic | 75.0% | |
| CGCCGGAGAAACACCGGCGG+TGG | + | chr8.4:28524772-28524791 | None:intergenic | 75.0% | |
| GCCGCCTGCGAACTCGTCGG+CGG | - | chr8.4:28524546-28524565 | MS.gene28490:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 28524165 | 28525277 | 28524165 | ID=MS.gene28490 |
| chr8.4 | mRNA | 28524165 | 28525277 | 28524165 | ID=MS.gene28490.t1;Parent=MS.gene28490 |
| chr8.4 | exon | 28524165 | 28525277 | 28524165 | ID=MS.gene28490.t1.exon1;Parent=MS.gene28490.t1 |
| chr8.4 | CDS | 28524165 | 28525277 | 28524165 | ID=cds.MS.gene28490.t1;Parent=MS.gene28490.t1 |
| Gene Sequence |
| Protein sequence |
