AlfalfaGEDB | MS.gene061877

M. sativa cultivar XinJiangDaYe / MS.gene061877

KEGG: K03062
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061877.t1	XP_004493865.1	87.6	177	8	2	20	192	244	410	8.80E-77	296.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061877.t1	P46466	85.9	177	11	2	20	192	246	412	6.8e-78	291.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene061877.t1	A0A072U2S5	87.6	177	8	2	20	192	243	409	6.4e-77	296.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene061877	0.840045	1.10E-57	-1.69E-46
MS.gene049159	MS.gene061877	0.844188	8.82E-59	-1.69E-46
MS.gene049255	MS.gene061877	0.853316	2.59E-61	-1.69E-46
MS.gene049341	MS.gene061877	0.802023	6.83E-49	-1.69E-46
MS.gene049672	MS.gene061877	0.837617	4.68E-57	-1.69E-46
MS.gene049821	MS.gene061877	0.803319	3.69E-49	-1.69E-46
MS.gene049850	MS.gene061877	0.806984	6.31E-50	-1.69E-46
MS.gene050437	MS.gene061877	0.811803	5.83E-51	-1.69E-46
MS.gene05045	MS.gene061877	0.832091	1.15E-55	-1.69E-46
MS.gene050689	MS.gene061877	0.867194	1.64E-65	-1.69E-46
MS.gene050815	MS.gene061877	0.815271	1.01E-51	-1.69E-46
MS.gene051003	MS.gene061877	0.826699	2.35E-54	-1.69E-46
MS.gene051247	MS.gene061877	0.801167	1.02E-48	-1.69E-46
MS.gene051328	MS.gene061877	0.847039	1.49E-59	-1.69E-46
MS.gene05134	MS.gene061877	0.835748	1.40E-56	-1.69E-46
MS.gene051719	MS.gene061877	0.81483	1.26E-51	-1.69E-46
MS.gene051720	MS.gene061877	0.802277	6.05E-49	-1.69E-46
MS.gene051744	MS.gene061877	0.8184	2.00E-52	-1.69E-46
MS.gene052516	MS.gene061877	0.841667	4.14E-58	-1.69E-46
MS.gene052519	MS.gene061877	0.840284	9.54E-58	-1.69E-46
MS.gene052611	MS.gene061877	0.833447	5.31E-56	-1.69E-46
MS.gene052773	MS.gene061877	0.812245	4.67E-51	-1.69E-46
MS.gene052863	MS.gene061877	0.844717	6.36E-59	-1.69E-46
MS.gene053008	MS.gene061877	0.844196	8.78E-59	-1.69E-46
MS.gene053122	MS.gene061877	0.806653	7.41E-50	-1.69E-46
MS.gene053226	MS.gene061877	0.844201	8.75E-59	-1.69E-46
MS.gene05364	MS.gene061877	0.826934	2.07E-54	-1.69E-46
MS.gene054142	MS.gene061877	0.80204	6.78E-49	-1.69E-46
MS.gene054651	MS.gene061877	0.817042	4.05E-52	-1.69E-46
MS.gene055162	MS.gene061877	0.815352	9.66E-52	-1.69E-46
MS.gene05517	MS.gene061877	0.812465	4.18E-51	-1.69E-46
MS.gene055466	MS.gene061877	0.826871	2.14E-54	-1.69E-46
MS.gene055468	MS.gene061877	0.814696	1.35E-51	-1.69E-46
MS.gene055512	MS.gene061877	0.811937	5.45E-51	-1.69E-46
MS.gene055818	MS.gene061877	0.815235	1.03E-51	-1.69E-46
MS.gene056215	MS.gene061877	0.839842	1.24E-57	-1.69E-46
MS.gene056595	MS.gene061877	0.846634	1.92E-59	-1.69E-46
MS.gene057140	MS.gene061877	0.800156	1.65E-48	-1.69E-46
MS.gene057693	MS.gene061877	0.83884	2.26E-57	-1.69E-46
MS.gene057694	MS.gene061877	0.809617	1.73E-50	-1.69E-46
MS.gene058009	MS.gene061877	0.827403	1.60E-54	-1.69E-46
MS.gene058010	MS.gene061877	0.80131	9.56E-49	-1.69E-46
MS.gene058783	MS.gene061877	0.845829	3.18E-59	-1.69E-46
MS.gene058976	MS.gene061877	0.811166	8.02E-51	-1.69E-46
MS.gene059151	MS.gene061877	0.877466	6.14E-69	-1.69E-46
MS.gene05978	MS.gene061877	0.80008	1.70E-48	-1.69E-46
MS.gene059842	MS.gene061877	0.840826	6.88E-58	-1.69E-46
MS.gene060429	MS.gene061877	0.846787	1.74E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 32 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTCCCTCACAAGTTT+TGG	0.248853	7.4:-10631688	None:intergenic
GCATGTGATCTTTACTTGTA+TGG	0.352849	7.4:+10631460	None:intergenic
CAATTATATGCATTAATTAA+CGG	0.377725	7.4:+10631487	MS.gene061877:CDS
TAGAATCAAAACCATCTAAC+TGG	0.394542	7.4:-10631995	None:intergenic
AGAAGAATTTGTCATGACTA+AGG	0.410103	7.4:+10632293	MS.gene061877:CDS
ATCAAGGCTTTCAATTCTGT+TGG	0.426673	7.4:-10632045	None:intergenic
AAATTTAGATACATACATCT+AGG	0.429419	7.4:+10632244	MS.gene061877:intron
GCAACATTCTTGCGTGTTGT+TGG	0.429964	7.4:+10631631	MS.gene061877:CDS
GAGTTGCTGAACCAGTTAGA+TGG	0.449762	7.4:+10631984	MS.gene061877:CDS
TCATCAATAAAGAACAATAG+AGG	0.453866	7.4:-10631734	None:intergenic
ATGACTAAGGATGAGTTCTC+TGG	0.469513	7.4:+10632306	MS.gene061877:CDS
AGTAACTTCTAACCTTCATT+CGG	0.472587	7.4:-10632384	None:intergenic
TTTACGAGAACGCCGAATGA+AGG	0.481823	7.4:+10632372	MS.gene061877:CDS
CGACCTGGTCTTAGCAGAGC+TGG	0.483758	7.4:-10632068	None:intergenic
GCGTTCTCGTAAAGCAAGTA+AGG	0.492903	7.4:-10632362	None:intergenic
AAAGCAATATGTACCGAAGC+TGG	0.494002	7.4:+10632339	MS.gene061877:CDS
TAAGACCAGGTCGAATAGAC+AGG	0.501709	7.4:+10632078	MS.gene061877:CDS
AAAGCAAGTAAGGCCAGCTT+CGG	0.515852	7.4:-10632352	None:intergenic
TACTTTCTGATATGCCATGC+AGG	0.522279	7.4:+10631913	MS.gene061877:CDS
GTCTTAGCAGAGCTGGATCA+AGG	0.525057	7.4:-10632061	None:intergenic
CTCTCCCTGATATCAAAACT+AGG	0.526151	7.4:+10632114	MS.gene061877:CDS
GAATGTTGCTGATGTTGAGT+TGG	0.578313	7.4:-10631617	None:intergenic
GTGGAGAGCGTGAAATTCAA+AGG	0.587676	7.4:+10631952	MS.gene061877:CDS
AGATGGTCCAAAACTTGTGA+GGG	0.588030	7.4:+10631681	MS.gene061877:CDS
CATCAATAAAGAACAATAGA+GGG	0.592647	7.4:-10631733	None:intergenic
TGCAGGTATGATGCTCACTC+AGG	0.601841	7.4:+10631930	MS.gene061877:CDS
TGAAATTCAAAGGACCATGT+TGG	0.608156	7.4:+10631962	MS.gene061877:CDS
CTGGTTCAGCAACTCCAACA+TGG	0.624925	7.4:-10631976	None:intergenic
GATCCAGCTCTGCTAAGACC+AGG	0.627123	7.4:+10632065	MS.gene061877:CDS
GAGATGGTCCAAAACTTGTG+AGG	0.639633	7.4:+10631680	MS.gene061877:CDS
GAGTGAGCATCATACCTGCA+TGG	0.657964	7.4:-10631927	None:intergenic
AGGTATGATGCTCACTCAGG+TGG	0.668411	7.4:+10631933	MS.gene061877:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAATTATATGCATTAATTAA+CGG	+	chr7.4:10631487-10631506	MS.gene061877:CDS	15.0%
!!	AAATTTAGATACATACATCT+AGG	+	chr7.4:10632244-10632263	MS.gene061877:intron	20.0%
!!!	GAATTGATTCAAAAATACTT+AGG	+	chr7.4:10631658-10631677	MS.gene061877:CDS	20.0%
!	ATTCAAAAATACTTAGGAGA+TGG	+	chr7.4:10631664-10631683	MS.gene061877:CDS	25.0%
!	CATCAATAAAGAACAATAGA+GGG	-	chr7.4:10631736-10631755	None:intergenic	25.0%
!	TCATCAATAAAGAACAATAG+AGG	-	chr7.4:10631737-10631756	None:intergenic	25.0%
	AGAAGAATTTGTCATGACTA+AGG	+	chr7.4:10632293-10632312	MS.gene061877:CDS	30.0%
	TAGAATCAAAACCATCTAAC+TGG	-	chr7.4:10631998-10632017	None:intergenic	30.0%
!!	TTGAATGTGGCATTTTGTTT+TGG	+	chr7.4:10631834-10631853	MS.gene061877:intron	30.0%
!!!	TGAATGTGGCATTTTGTTTT+GGG	+	chr7.4:10631835-10631854	MS.gene061877:intron	30.0%
!!!	TTAGATGGTTTTGATTCTAG+AGG	+	chr7.4:10631999-10632018	MS.gene061877:CDS	30.0%
	AATGTGTCCTTTCTTGAATG+TGG	+	chr7.4:10631821-10631840	MS.gene061877:intron	35.0%
	ATCAAGGCTTTCAATTCTGT+TGG	-	chr7.4:10632048-10632067	None:intergenic	35.0%
	ATTGATGAAATTGACGCTGT+TGG	+	chr7.4:10631749-10631768	MS.gene061877:intron	35.0%
	CAAAATGCCACATTCAAGAA+AGG	-	chr7.4:10631831-10631850	None:intergenic	35.0%
	CGTTATTGAGTATCAGCTTA+AGG	+	chr7.4:10631784-10631803	MS.gene061877:intron	35.0%
	TGAAATTCAAAGGACCATGT+TGG	+	chr7.4:10631962-10631981	MS.gene061877:CDS	35.0%
!	AAAAGTTCCCTCACAAGTTT+TGG	-	chr7.4:10631691-10631710	None:intergenic	35.0%
!!!	GTCTCCTAGTTTTGATATCA+GGG	-	chr7.4:10632121-10632140	None:intergenic	35.0%
	AAAGCAATATGTACCGAAGC+TGG	+	chr7.4:10632339-10632358	MS.gene061877:CDS	40.0%
	AGATGGTCCAAAACTTGTGA+GGG	+	chr7.4:10631681-10631700	MS.gene061877:CDS	40.0%
	ATGACTAAGGATGAGTTCTC+TGG	+	chr7.4:10632306-10632325	MS.gene061877:CDS	40.0%
	CTCTCCCTGATATCAAAACT+AGG	+	chr7.4:10632114-10632133	MS.gene061877:CDS	40.0%
	GAATGTTGCTGATGTTGAGT+TGG	-	chr7.4:10631620-10631639	None:intergenic	40.0%
	TACTTTCTGATATGCCATGC+AGG	+	chr7.4:10631913-10631932	MS.gene061877:CDS	40.0%
!	AACTAGGAGACGCATTTTCA+CGG	+	chr7.4:10632130-10632149	MS.gene061877:CDS	40.0%
!	ATTTTCCTGTCTATTCGACC+TGG	-	chr7.4:10632086-10632105	None:intergenic	40.0%
!!!	CGTCTCCTAGTTTTGATATC+AGG	-	chr7.4:10632122-10632141	None:intergenic	40.0%
!!!	CTAGTTTTGATATCAGGGAG+AGG	-	chr7.4:10632116-10632135	None:intergenic	40.0%
	AAAGCAAGTAAGGCCAGCTT+CGG	-	chr7.4:10632355-10632374	None:intergenic	45.0%
	GAGATGGTCCAAAACTTGTG+AGG	+	chr7.4:10631680-10631699	MS.gene061877:CDS	45.0%
	GAGTTGCTGAACCAGTTAGA+TGG	+	chr7.4:10631984-10632003	MS.gene061877:CDS	45.0%
	GCAACATTCTTGCGTGTTGT+TGG	+	chr7.4:10631631-10631650	MS.gene061877:CDS	45.0%
	GCGTTCTCGTAAAGCAAGTA+AGG	-	chr7.4:10632365-10632384	None:intergenic	45.0%
	TAAGACCAGGTCGAATAGAC+AGG	+	chr7.4:10632078-10632097	MS.gene061877:CDS	45.0%
	TTGACGCTGTTGGTACAAAG+AGG	+	chr7.4:10631759-10631778	MS.gene061877:intron	45.0%
	TTTACGAGAACGCCGAATGA+AGG	+	chr7.4:10632372-10632391	MS.gene061877:CDS	45.0%
!	AGAGTGCTTCCTTTGCTTTC+AGG	+	chr7.4:10631588-10631607	MS.gene061877:intron	45.0%
!	GTGGAGAGCGTGAAATTCAA+AGG	+	chr7.4:10631952-10631971	MS.gene061877:CDS	45.0%
!!!	GCATTTTGTTTTGGGCGTAG+GGG	+	chr7.4:10631843-10631862	MS.gene061877:intron	45.0%
!!!	GGCATTTTGTTTTGGGCGTA+GGG	+	chr7.4:10631842-10631861	MS.gene061877:intron	45.0%
!!!	TGGCATTTTGTTTTGGGCGT+AGG	+	chr7.4:10631841-10631860	MS.gene061877:intron	45.0%
	AGGTATGATGCTCACTCAGG+TGG	+	chr7.4:10631933-10631952	MS.gene061877:CDS	50.0%
	CTGGTTCAGCAACTCCAACA+TGG	-	chr7.4:10631979-10631998	None:intergenic	50.0%
	CTTCCTTTGCTTTCAGGCTG+TGG	+	chr7.4:10631594-10631613	MS.gene061877:intron	50.0%
	GAGTGAGCATCATACCTGCA+TGG	-	chr7.4:10631930-10631949	None:intergenic	50.0%
	GTCTTAGCAGAGCTGGATCA+AGG	-	chr7.4:10632064-10632083	None:intergenic	50.0%
	TGCAGGTATGATGCTCACTC+AGG	+	chr7.4:10631930-10631949	MS.gene061877:CDS	50.0%
!	AGGAGACGCATTTTCACGGT+AGG	+	chr7.4:10632134-10632153	MS.gene061877:intron	50.0%
	GATCCAGCTCTGCTAAGACC+AGG	+	chr7.4:10632065-10632084	MS.gene061877:CDS	55.0%
	TGGCCACAGCCTGAAAGCAA+AGG	-	chr7.4:10631600-10631619	None:intergenic	55.0%
!!	TTTTGGGCGTAGGGGTGTGT+GGG	+	chr7.4:10631851-10631870	MS.gene061877:intron	55.0%
!	GTAGGGGTGTGTGGGAACAG+TGG	+	chr7.4:10631859-10631878	MS.gene061877:intron	60.0%
!!	CGACCTGGTCTTAGCAGAGC+TGG	-	chr7.4:10632071-10632090	None:intergenic	60.0%
!!	GTTTTGGGCGTAGGGGTGTG+TGG	+	chr7.4:10631850-10631869	MS.gene061877:intron	60.0%
	GGGGTGTGTGGGAACAGTGG+AGG	+	chr7.4:10631862-10631881	MS.gene061877:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	10631461	10632398	10631461	ID=MS.gene061877
chr7.4	mRNA	10631461	10632398	10631461	ID=MS.gene061877.t1;Parent=MS.gene061877
chr7.4	exon	10631461	10631519	10631461	ID=MS.gene061877.t1.exon1;Parent=MS.gene061877.t1
chr7.4	CDS	10631461	10631519	10631461	ID=cds.MS.gene061877.t1;Parent=MS.gene061877.t1
chr7.4	exon	10631610	10631741	10631610	ID=MS.gene061877.t1.exon2;Parent=MS.gene061877.t1
chr7.4	CDS	10631610	10631741	10631610	ID=cds.MS.gene061877.t1;Parent=MS.gene061877.t1
chr7.4	exon	10631879	10632151	10631879	ID=MS.gene061877.t1.exon3;Parent=MS.gene061877.t1
chr7.4	CDS	10631879	10632151	10631879	ID=cds.MS.gene061877.t1;Parent=MS.gene061877.t1
chr7.4	exon	10632252	10632398	10632252	ID=MS.gene061877.t1.exon4;Parent=MS.gene061877.t1
chr7.4	CDS	10632252	10632398	10632252	ID=cds.MS.gene061877.t1;Parent=MS.gene061877.t1

Gene Sequence

>MS.gene061877

CATGTGATCTTTACTTGTATGGACAGCAATTATATGCATTAATTAACGGATGTCTAACTGTAACTTGTTATTATTATTGCATTTTATGATTTTAGATATTACACTCTGCACAGTCTGTTTGATCTAAAGAGTGCTTCCTTTGCTTTCAGGCTGTGGCCAACTCAACATCAGCAACATTCTTGCGTGTTGTTGGTAGTGAATTGATTCAAAAATACTTAGGAGATGGTCCAAAACTTGTGAGGGAACTTTTTCGAGTTGCTGATGATCTTTCTCCCTCTATTGTTCTTTATTGATGAAATTGACGCTGTTGGTACAAAGAGGTACGTTATTGAGTATCAGCTTAAGGATTCGCATTGTAATAATGTGTCCTTTCTTGAATGTGGCATTTTGTTTTGGGCGTAGGGGTGTGTGGGAACAGTGGAGGAGTTGTACATATAAATCATGTCTTATTATACTTTCTGATATGCCATGCAGGTATGATGCTCACTCAGGTGGAGAGCGTGAAATTCAAAGGACCATGTTGGAGTTGCTGAACCAGTTAGATGGTTTTGATTCTAGAGGAGATGTTAAAGTTATTCTCGCAACCAACAGAATTGAAAGCCTTGATCCAGCTCTGCTAAGACCAGGTCGAATAGACAGGAAAATTGAATTTCCTCTCCCTGATATCAAAACTAGGAGACGCATTTTCACGGTAGGCGTCAATTGCGAAAAGTAGCACACTTGTTGTTTTAGCTGATATTGTGATATTTTATATCTGTTTGACTGAAACATCTCATACTTTTGAAATTTAGATACATACATCTAGGATGACATTAGCTGACGATGTCAATTTAGAAGAATTTGTCATGACTAAGGATGAGTTCTCTGGAGCTGATATAAAAGCAATATGTACCGAAGCTGGCCTTACTTGCTTTACGAGAACGCCGAATGAAGGTTAG

Protein sequence

>MS.gene061877.t1

HVIFTCMDSNYMHULTDVULLWPTQHQQHSCVLLVVNUFKNTUEMVQNLUGNFFELLMIFLPLFGGVVHINHVLLYFLICHAGMMLTQVESVKFKGPCWSCUTSUMVLILEEMLKLFSQPTELKALIQLCUDQVEUTGKLNFLSLISKLGDAFSRYIHLGUHULTMSIUKNLSULRMSSLELIUKQYVPKLALLALRERRMKV