Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063035.t1 | XP_013456479.1 | 98.7 | 238 | 3 | 0 | 1 | 238 | 6 | 243 | 3.50E-133 | 484.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063035.t1 | A0A1P8B554 | 68.8 | 250 | 67 | 4 | 1 | 239 | 11 | 260 | 4.7e-94 | 345.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063035.t1 | A0A072UXB7 | 98.7 | 238 | 3 | 0 | 1 | 238 | 6 | 243 | 2.5e-133 | 484.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene063035 | 0.808125 | 3.61E-50 | -1.69E-46 |
| MS.gene049622 | MS.gene063035 | 0.81794 | 2.54E-52 | -1.69E-46 |
| MS.gene049989 | MS.gene063035 | 0.845871 | 3.10E-59 | -1.69E-46 |
| MS.gene050297 | MS.gene063035 | 0.818819 | 1.61E-52 | -1.69E-46 |
| MS.gene05054 | MS.gene063035 | 0.806325 | 8.69E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene063035 | 0.810272 | 1.25E-50 | -1.69E-46 |
| MS.gene051742 | MS.gene063035 | 0.837302 | 5.63E-57 | -1.69E-46 |
| MS.gene051816 | MS.gene063035 | 0.826468 | 2.67E-54 | -1.69E-46 |
| MS.gene051955 | MS.gene063035 | 0.833381 | 5.51E-56 | -1.69E-46 |
| MS.gene052143 | MS.gene063035 | 0.842932 | 1.91E-58 | -1.69E-46 |
| MS.gene052169 | MS.gene063035 | 0.816251 | 6.09E-52 | -1.69E-46 |
| MS.gene052308 | MS.gene063035 | 0.877865 | 4.46E-69 | -1.69E-46 |
| MS.gene052367 | MS.gene063035 | 0.883545 | 4.10E-71 | -1.69E-46 |
| MS.gene052383 | MS.gene063035 | 0.809499 | 1.84E-50 | -1.69E-46 |
| MS.gene053173 | MS.gene063035 | 0.852637 | 4.05E-61 | -1.69E-46 |
| MS.gene053348 | MS.gene063035 | 0.860694 | 1.73E-63 | -1.69E-46 |
| MS.gene053416 | MS.gene063035 | 0.834759 | 2.49E-56 | -1.69E-46 |
| MS.gene053417 | MS.gene063035 | 0.834496 | 2.90E-56 | -1.69E-46 |
| MS.gene05369 | MS.gene063035 | 0.80139 | 9.21E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene063035 | 0.869282 | 3.48E-66 | -1.69E-46 |
| MS.gene054552 | MS.gene063035 | 0.806451 | 8.17E-50 | -1.69E-46 |
| MS.gene054593 | MS.gene063035 | 0.866593 | 2.55E-65 | -1.69E-46 |
| MS.gene054614 | MS.gene063035 | 0.829422 | 5.19E-55 | -1.69E-46 |
| MS.gene054941 | MS.gene063035 | 0.832083 | 1.16E-55 | -1.69E-46 |
| MS.gene055308 | MS.gene063035 | 0.873541 | 1.36E-67 | -1.69E-46 |
| MS.gene055578 | MS.gene063035 | 0.869147 | 3.85E-66 | -1.69E-46 |
| MS.gene055579 | MS.gene063035 | 0.844106 | 9.28E-59 | -1.69E-46 |
| MS.gene055957 | MS.gene063035 | 0.858302 | 9.04E-63 | -1.69E-46 |
| MS.gene056041 | MS.gene063035 | 0.909195 | 7.45E-82 | -1.69E-46 |
| MS.gene056042 | MS.gene063035 | 0.848169 | 7.27E-60 | -1.69E-46 |
| MS.gene05640 | MS.gene063035 | 0.866167 | 3.47E-65 | -1.69E-46 |
| MS.gene056526 | MS.gene063035 | 0.859981 | 2.84E-63 | -1.69E-46 |
| MS.gene056528 | MS.gene063035 | 0.846628 | 1.93E-59 | -1.69E-46 |
| MS.gene05652 | MS.gene063035 | 0.844928 | 5.58E-59 | -1.69E-46 |
| MS.gene057458 | MS.gene063035 | 0.863776 | 1.96E-64 | -1.69E-46 |
| MS.gene057669 | MS.gene063035 | 0.813734 | 2.20E-51 | -1.69E-46 |
| MS.gene058128 | MS.gene063035 | 0.839471 | 1.55E-57 | -1.69E-46 |
| MS.gene058196 | MS.gene063035 | 0.801513 | 8.69E-49 | -1.69E-46 |
| MS.gene058827 | MS.gene063035 | 0.82953 | 4.89E-55 | -1.69E-46 |
| MS.gene058828 | MS.gene063035 | 0.814162 | 1.77E-51 | -1.69E-46 |
| MS.gene058849 | MS.gene063035 | 0.836509 | 8.98E-57 | -1.69E-46 |
| MS.gene058853 | MS.gene063035 | 0.869668 | 2.61E-66 | -1.69E-46 |
| MS.gene059300 | MS.gene063035 | 0.810978 | 8.81E-51 | -1.69E-46 |
| MS.gene059553 | MS.gene063035 | 0.862642 | 4.39E-64 | -1.69E-46 |
| MS.gene059616 | MS.gene063035 | 0.815732 | 7.95E-52 | -1.69E-46 |
| MS.gene060473 | MS.gene063035 | 0.905867 | 2.73E-80 | -1.69E-46 |
| MS.gene060565 | MS.gene063035 | 0.893732 | 4.78E-75 | -1.69E-46 |
| MS.gene06057 | MS.gene063035 | 0.807998 | 3.84E-50 | -1.69E-46 |
| MS.gene060825 | MS.gene063035 | 0.824342 | 8.50E-54 | -1.69E-46 |
| MS.gene06093 | MS.gene063035 | 0.870509 | 1.38E-66 | -1.69E-46 |
| MS.gene061099 | MS.gene063035 | 0.85594 | 4.50E-62 | -1.69E-46 |
| MS.gene061260 | MS.gene063035 | 0.88289 | 7.13E-71 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTAAAACATAGACAA+AGG | + | 33363:13706-13725 | None:intergenic | 15.0% |
| !!! | TTTTTAACATGTTTTTGTTC+AGG | - | 33363:13763-13782 | MS.gene063035:CDS | 20.0% |
| AAAGAGAGTGATATAAGTTG+AGG | + | 33363:13646-13665 | None:intergenic | 30.0% | |
| !! | AAAGGTTGTGTTTTTACCTT+TGG | + | 33363:13688-13707 | None:intergenic | 30.0% |
| !! | AAGGTTGTGTTTTTACCTTT+GGG | + | 33363:13687-13706 | None:intergenic | 30.0% |
| !!! | GTTAGCGTTTTTTGTTTTGA+AGG | + | 33363:13733-13752 | None:intergenic | 30.0% |
| AACATTTCCGAGTAAATCGA+CGG | + | 33363:14327-14346 | None:intergenic | 35.0% | |
| AATCTACCCATGATTGTTGA+AGG | - | 33363:14129-14148 | MS.gene063035:CDS | 35.0% | |
| AGAAGATGGACAAAAGGTAA+CGG | + | 33363:14430-14449 | None:intergenic | 35.0% | |
| TCAAACATGGTAGAATGCAA+CGG | - | 33363:14015-14034 | MS.gene063035:CDS | 35.0% | |
| ! | TACTTTTACTTGTACTGCTG+CGG | - | 33363:14398-14417 | MS.gene063035:intron | 35.0% |
| !! | TGTTTTTGTTCAGGTATCAC+TGG | - | 33363:13772-13791 | MS.gene063035:CDS | 35.0% |
| AAGCGTAGCTATACGATCTA+GGG | + | 33363:14362-14381 | None:intergenic | 40.0% | |
| ACAAATGTGCCTACACAGTA+TGG | - | 33363:13815-13834 | MS.gene063035:CDS | 40.0% | |
| ACCAGAACCTTCAACAATCA+TGG | + | 33363:14139-14158 | None:intergenic | 40.0% | |
| CCAGAACCTTCAACAATCAT+GGG | + | 33363:14138-14157 | None:intergenic | 40.0% | |
| TAAGCGTAGCTATACGATCT+AGG | + | 33363:14363-14382 | None:intergenic | 40.0% | |
| ! | AGTCCTGAGTATTGTTGTAG+TGG | - | 33363:14273-14292 | MS.gene063035:CDS | 40.0% |
| ! | ATGATTGTTGAAGGTTCTGG+TGG | - | 33363:14138-14157 | MS.gene063035:CDS | 40.0% |
| ! | TGATTGTTGAAGGTTCTGGT+GGG | - | 33363:14139-14158 | MS.gene063035:CDS | 40.0% |
| !! | TGTTTTTACCTTTGGGAGTG+AGG | + | 33363:13680-13699 | None:intergenic | 40.0% |
| AAAGTGCGAGTGGTTTCTTG+TGG | + | 33363:13898-13917 | None:intergenic | 45.0% | |
| AATACCTGGCCATACTGTGT+AGG | + | 33363:13827-13846 | None:intergenic | 45.0% | |
| CCCATGATTGTTGAAGGTTC+TGG | - | 33363:14135-14154 | MS.gene063035:CDS | 45.0% | |
| CCTTGGAGAAGATGGACAAA+AGG | + | 33363:14436-14455 | None:intergenic | 45.0% | |
| GAGTTCACTCTTGGCACTAT+AGG | - | 33363:14069-14088 | MS.gene063035:CDS | 45.0% | |
| TATTGTTGTAGTGGCGCGTA+TGG | - | 33363:14282-14301 | MS.gene063035:CDS | 45.0% | |
| TTCTTGTGGAAGCTCGAAAC+CGG | + | 33363:13884-13903 | None:intergenic | 45.0% | |
| ! | CCTTTTGTCCATCTTCTCCA+AGG | - | 33363:14433-14452 | MS.gene063035:CDS | 45.0% |
| ! | CTTCTTTTCCTCACTCCCAA+AGG | - | 33363:13669-13688 | MS.gene063035:CDS | 45.0% |
| !! | TTGTAGGCCGTCGATTTACT+CGG | - | 33363:14317-14336 | MS.gene063035:intron | 45.0% |
| AATGCTGGTAGCCCAACACT+CGG | - | 33363:13853-13872 | MS.gene063035:CDS | 50.0% | |
| ACCCTAGCTGAGTTCACTCT+TGG | - | 33363:14060-14079 | MS.gene063035:CDS | 50.0% | |
| ATGGTAGAATGCAACGGTGC+CGG | - | 33363:14021-14040 | MS.gene063035:CDS | 50.0% | |
| CATGTTTGAGCCACAGTCTC+CGG | + | 33363:14004-14023 | None:intergenic | 50.0% | |
| CCAGCATTTGCGAGAATACC+TGG | + | 33363:13841-13860 | None:intergenic | 50.0% | |
| CCAGGTATTCTCGCAAATGC+TGG | - | 33363:13838-13857 | MS.gene063035:CDS | 50.0% | |
| GCCAAGAGTGAACTCAGCTA+GGG | + | 33363:14064-14083 | None:intergenic | 50.0% | |
| GCGCCACTACAACAATACTC+AGG | + | 33363:14279-14298 | None:intergenic | 50.0% | |
| GGATCTGAACCAGCAATGTC+CGG | - | 33363:14194-14213 | MS.gene063035:CDS | 50.0% | |
| TACGACGTGAGTCTCGTTGA+TGG | - | 33363:14102-14121 | MS.gene063035:CDS | 50.0% | |
| TGCCAAGAGTGAACTCAGCT+AGG | + | 33363:14065-14084 | None:intergenic | 50.0% | |
| ! | ATGGTTCACCGGCTACTTGT+AGG | - | 33363:14301-14320 | MS.gene063035:intron | 50.0% |
| ! | TGTTGAAGGTTCTGGTGGGT+CGG | - | 33363:14143-14162 | MS.gene063035:CDS | 50.0% |
| !! | AAGAGTGCGTGTGAGGCTTT+TGG | - | 33363:14249-14268 | MS.gene063035:CDS | 50.0% |
| AAACCGGTGCTTCCGAGTGT+TGG | + | 33363:13868-13887 | None:intergenic | 55.0% | |
| AACCGGTGCTTCCGAGTGTT+GGG | + | 33363:13867-13886 | None:intergenic | 55.0% | |
| AACTCAGCTAGGGTTGCTGG+TGG | + | 33363:14054-14073 | None:intergenic | 55.0% | |
| AACTTCGACGGATCCGGAAC+CGG | - | 33363:13967-13986 | MS.gene063035:CDS | 55.0% | |
| ATCGACGGCCTACAAGTAGC+CGG | + | 33363:14312-14331 | None:intergenic | 55.0% | |
| CGAACCGGTTGCAACTTCGA+CGG | - | 33363:13955-13974 | MS.gene063035:CDS | 55.0% | |
| CGGAGACTGTGGCTCAAACA+TGG | - | 33363:14002-14021 | MS.gene063035:CDS | 55.0% | |
| GCGGAGTTGAGAGCTTCAGA+TGG | - | 33363:14216-14235 | MS.gene063035:CDS | 55.0% | |
| GGATCCGTCGAAGTTGCAAC+CGG | + | 33363:13962-13981 | None:intergenic | 55.0% | |
| GGTTGCAACTTCGACGGATC+CGG | - | 33363:13961-13980 | MS.gene063035:CDS | 55.0% | |
| GTCTCCGGTGAGACATGAAC+CGG | + | 33363:13989-14008 | None:intergenic | 55.0% | |
| GTGAACTCAGCTAGGGTTGC+TGG | + | 33363:14057-14076 | None:intergenic | 55.0% | |
| GTTCAGATCCGACGTACACC+CGG | + | 33363:14184-14203 | None:intergenic | 55.0% | |
| TAGTGGCGCGTATGGTTCAC+CGG | - | 33363:14290-14309 | MS.gene063035:CDS | 55.0% | |
| TCATGTCTCACCGGAGACTG+TGG | - | 33363:13991-14010 | MS.gene063035:CDS | 55.0% | |
| TCGGGTATGTGTGAGTCCAC+CGG | - | 33363:14162-14181 | MS.gene063035:CDS | 55.0% | |
| TCTGAACCAGCAATGTCCGG+CGG | - | 33363:14197-14216 | MS.gene063035:CDS | 55.0% | |
| TGTGCCTACACAGTATGGCC+AGG | - | 33363:13820-13839 | MS.gene063035:CDS | 55.0% | |
| ! | GGAACCGGTTCATGTCTCAC+CGG | - | 33363:13982-14001 | MS.gene063035:CDS | 55.0% |
| ! | GGTGAGACATGAACCGGTTC+CGG | + | 33363:13983-14002 | None:intergenic | 55.0% |
| ! | GTTGAAGGTTCTGGTGGGTC+GGG | - | 33363:14144-14163 | MS.gene063035:CDS | 55.0% |
| ! | TCTGAAGCTCTCAACTCCGC+CGG | + | 33363:14216-14235 | None:intergenic | 55.0% |
| ! | TGGAGCTTGGAAAGTGCGAG+TGG | + | 33363:13908-13927 | None:intergenic | 55.0% |
| CGCACTTTCCAAGCTCCAGC+CGG | - | 33363:13910-13929 | MS.gene063035:CDS | 60.0% | |
| CGGGTATGTGTGAGTCCACC+GGG | - | 33363:14163-14182 | MS.gene063035:CDS | 60.0% | |
| CTTTCCAAGCTCCAGCCGGT+TGG | - | 33363:13914-13933 | MS.gene063035:CDS | 60.0% | |
| TCAACTCCGCCGGACATTGC+TGG | + | 33363:14206-14225 | None:intergenic | 60.0% | |
| TGAGTCCACCGGGTGTACGT+CGG | - | 33363:14173-14192 | MS.gene063035:CDS | 60.0% | |
| ! | AGCCCAACACTCGGAAGCAC+CGG | - | 33363:13862-13881 | MS.gene063035:CDS | 60.0% |
| ! | CGCGTGTAAGAGTGCGTGTG+AGG | - | 33363:14242-14261 | MS.gene063035:CDS | 60.0% |
| !! | AACCGGTTCGAGCCCAGAAC+CGG | + | 33363:13945-13964 | None:intergenic | 60.0% |
| CAGATCCGACGTACACCCGG+TGG | + | 33363:14181-14200 | None:intergenic | 65.0% | |
| ! | CAAGCTCCAGCCGGTTGGTC+CGG | - | 33363:13919-13938 | MS.gene063035:CDS | 65.0% |
| ! | GGTTGCTGGTGGTGCAGCTC+CGG | + | 33363:14043-14062 | None:intergenic | 65.0% |
| CCAGAACCGGCCGGACCAAC+CGG | + | 33363:13932-13951 | None:intergenic | 70.0% | |
| CGGACCAACCGGCTGGAGCT+TGG | + | 33363:13921-13940 | None:intergenic | 70.0% | |
| GGTTCGAGCCCAGAACCGGC+CGG | + | 33363:13941-13960 | None:intergenic | 70.0% | |
| ! | CGGTTGGTCCGGCCGGTTCT+GGG | - | 33363:13930-13949 | MS.gene063035:CDS | 70.0% |
| !! | GGCCGGTTCTGGGCTCGAAC+CGG | - | 33363:13940-13959 | MS.gene063035:CDS | 70.0% |
| AACCGGCCGGACCAACCGGC+TGG | + | 33363:13928-13947 | None:intergenic | 75.0% | |
| ! | CCGGTTGGTCCGGCCGGTTC+TGG | - | 33363:13929-13948 | MS.gene063035:CDS | 75.0% |
| ! | CTCCAGCCGGTTGGTCCGGC+CGG | - | 33363:13923-13942 | MS.gene063035:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33363 | gene | 13643 | 14458 | 13643 | ID=MS.gene063035 |
| 33363 | mRNA | 13643 | 14458 | 13643 | ID=MS.gene063035.t1;Parent=MS.gene063035 |
| 33363 | exon | 14411 | 14458 | 14411 | ID=MS.gene063035.t1.exon1;Parent=MS.gene063035.t1 |
| 33363 | CDS | 14411 | 14458 | 14411 | ID=cds.MS.gene063035.t1;Parent=MS.gene063035.t1 |
| 33363 | exon | 13643 | 14316 | 13643 | ID=MS.gene063035.t1.exon2;Parent=MS.gene063035.t1 |
| 33363 | CDS | 13643 | 14316 | 13643 | ID=cds.MS.gene063035.t1;Parent=MS.gene063035.t1 |
| Gene Sequence |
| Protein sequence |
