AlfalfaGEDB | MS.gene065713

M. sativa cultivar XinJiangDaYe / MS.gene065713

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065713.t1	XP_024629609.1	81.1	106	9	2	1	96	1	105	1.20E-34	155.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065713.t1	A0A072VKL8	81.1	106	9	2	1	96	1	105	8.6e-35	155.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050301	MS.gene065713	0.810715	1.00E-50	-1.69E-46
MS.gene050397	MS.gene065713	0.816304	5.92E-52	-1.69E-46
MS.gene050400	MS.gene065713	0.811528	6.69E-51	-1.69E-46
MS.gene050404	MS.gene065713	0.806478	8.07E-50	-1.69E-46
MS.gene050406	MS.gene065713	0.806026	1.00E-49	-1.69E-46
MS.gene050464	MS.gene065713	0.803194	3.92E-49	-1.69E-46
MS.gene050529	MS.gene065713	0.807131	5.87E-50	-1.69E-46
MS.gene051471	MS.gene065713	0.805425	1.34E-49	-1.69E-46
MS.gene051582	MS.gene065713	0.812189	4.80E-51	-1.69E-46
MS.gene052011	MS.gene065713	-0.804045	2.61E-49	-1.69E-46
MS.gene052078	MS.gene065713	0.82248	2.32E-53	-1.69E-46
MS.gene052178	MS.gene065713	0.835994	1.21E-56	-1.69E-46
MS.gene052188	MS.gene065713	0.815546	8.75E-52	-1.69E-46
MS.gene052253	MS.gene065713	0.813236	2.83E-51	-1.69E-46
MS.gene052310	MS.gene065713	0.820355	7.16E-53	-1.69E-46
MS.gene052322	MS.gene065713	0.808039	3.77E-50	-1.69E-46
MS.gene052340	MS.gene065713	0.832625	8.50E-56	-1.69E-46
MS.gene053557	MS.gene065713	0.816584	5.13E-52	-1.69E-46
MS.gene053836	MS.gene065713	0.811978	5.34E-51	-1.69E-46
MS.gene053928	MS.gene065713	0.808793	2.60E-50	-1.69E-46
MS.gene054011	MS.gene065713	0.811644	6.32E-51	-1.69E-46
MS.gene054012	MS.gene065713	0.801008	1.10E-48	-1.69E-46
MS.gene054349	MS.gene065713	0.801249	9.84E-49	-1.69E-46
MS.gene055277	MS.gene065713	0.815443	9.22E-52	-1.69E-46
MS.gene055422	MS.gene065713	0.81728	3.58E-52	-1.69E-46
MS.gene055428	MS.gene065713	0.829265	5.67E-55	-1.69E-46
MS.gene05553	MS.gene065713	0.833381	5.51E-56	-1.69E-46
MS.gene055604	MS.gene065713	0.8094	1.93E-50	-1.69E-46
MS.gene056191	MS.gene065713	0.801844	7.43E-49	-1.69E-46
MS.gene056612	MS.gene065713	0.81466	1.38E-51	-1.69E-46
MS.gene056796	MS.gene065713	0.802629	5.12E-49	-1.69E-46
MS.gene056899	MS.gene065713	-0.801777	7.67E-49	-1.69E-46
MS.gene05702	MS.gene065713	0.829789	4.23E-55	-1.69E-46
MS.gene057825	MS.gene065713	0.807556	4.77E-50	-1.69E-46
MS.gene058002	MS.gene065713	0.802017	6.85E-49	-1.69E-46
MS.gene05811	MS.gene065713	0.834423	3.02E-56	-1.69E-46
MS.gene058142	MS.gene065713	0.826574	2.52E-54	-1.69E-46
MS.gene058233	MS.gene065713	0.815976	7.01E-52	-1.69E-46
MS.gene058276	MS.gene065713	0.826232	3.04E-54	-1.69E-46
MS.gene05907	MS.gene065713	0.821278	4.40E-53	-1.69E-46
MS.gene059294	MS.gene065713	0.831193	1.92E-55	-1.69E-46
MS.gene05984	MS.gene065713	0.832464	9.31E-56	-1.69E-46
MS.gene059894	MS.gene065713	0.838455	2.85E-57	-1.69E-46
MS.gene060407	MS.gene065713	0.806594	7.63E-50	-1.69E-46
MS.gene061049	MS.gene065713	0.857	2.20E-62	-1.69E-46
MS.gene061159	MS.gene065713	0.802145	6.45E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065713.t1	MTR_1g058120	81.132	106	9	2	1	96	1	105	2.25e-52	160
MS.gene065713.t1	MTR_7g105730	57.843	102	18	4	1	92	14	100	3.80e-22	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065713.t1	AT3G11600	53.571	84	23	3	1	82	1	70	1.65e-17	72.0
MS.gene065713.t1	AT5G06270	57.547	106	29	3	1	96	1	100	3.86e-17	71.2
MS.gene065713.t1	AT5G06270	57.547	106	29	3	1	96	1	100	3.86e-17	71.2

Find 30 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAGCCACTTCCATGGTTT+TGG	0.236114	1.2:-45697966	MS.gene065713:CDS
GCTGGTTGTTTCCATCTTCT+TGG	0.237222	1.2:+45698011	None:intergenic
AAAACCATGGAAGTGGCTTC+TGG	0.299603	1.2:+45697968	None:intergenic
TTAAGTTTCAAGTCAAGTTT+TGG	0.305467	1.2:+45698142	None:intergenic
AAACCATGGAAGTGGCTTCT+GGG	0.413561	1.2:+45697969	None:intergenic
AAGAATGCAGAACTGGCAAC+AGG	0.414521	1.2:+45697662	None:intergenic
AAAACAATGAGTCGAAGAAA+TGG	0.423682	1.2:-45698172	None:intergenic
GACACTGTTGCTGATCTTGT+TGG	0.430360	1.2:+45698076	None:intergenic
CTAGCAGAAGAATGCAGAAC+TGG	0.446661	1.2:+45697655	None:intergenic
TTCTGCATTCTTCTGCTAGT+AGG	0.457170	1.2:-45697651	MS.gene065713:CDS
ACTCTTCTGTGGTCAACCCT+TGG	0.466923	1.2:+45698109	None:intergenic
ATCTTCTTGGTTTAGCTCAG+TGG	0.472861	1.2:+45698024	None:intergenic
TGTTAGAGTATCTCTGCTGC+TGG	0.501808	1.2:+45697993	None:intergenic
CTTCTGTGGTCAACCCTTGG+TGG	0.502015	1.2:+45698112	None:intergenic
TGGACACACATGAGCTTGGT+GGG	0.504715	1.2:+45698044	None:intergenic
GGTCAACCCTTGGTGGTGAT+AGG	0.515699	1.2:+45698119	None:intergenic
CATGAGCTTGGTGGGGATGT+AGG	0.527413	1.2:+45698052	None:intergenic
GTGGACACACATGAGCTTGG+TGG	0.535227	1.2:+45698043	None:intergenic
ACTGAGCTAAACCAAGAAGA+TGG	0.545142	1.2:-45698022	MS.gene065713:CDS
ACCAACCAAAACCATGGAAG+TGG	0.557850	1.2:+45697961	None:intergenic
CAGCCCAGAAGCCACTTCCA+TGG	0.578825	1.2:-45697972	MS.gene065713:CDS
TCAGTGGACACACATGAGCT+TGG	0.580086	1.2:+45698040	None:intergenic
GTGATGACTCAACTCTTCTG+TGG	0.595889	1.2:+45698098	None:intergenic
ATGAGTCGAAGAAATGGAAG+TGG	0.602019	1.2:-45698166	MS.gene065713:CDS
AGGACAACCAACCAAAACCA+TGG	0.614893	1.2:+45697955	None:intergenic
AACTTAACCTATCACCACCA+AGG	0.641567	1.2:-45698126	MS.gene065713:CDS
ACTTAACCTATCACCACCAA+GGG	0.643165	1.2:-45698125	MS.gene065713:CDS
AGAATGCAGAACTGGCAACA+GGG	0.650012	1.2:+45697663	None:intergenic
GGACACACATGAGCTTGGTG+GGG	0.713842	1.2:+45698045	None:intergenic
ATGACATACATTAGACAACG+AGG	0.778362	1.2:+45697935	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTAGTTTTTGCCAAAATTA+CGG	-	chr1.2:45698053-45698072	MS.gene065713:CDS	25.0%
!!!	TTAAGTTTCAAGTCAAGTTT+TGG	+	chr1.2:45697662-45697681	None:intergenic	25.0%
	ATGAAAACAACAACTCCAAA+AGG	-	chr1.2:45697951-45697970	MS.gene065713:CDS	30.0%
!	AAAACAGTGCTTTTGCATTT+AGG	+	chr1.2:45697917-45697936	None:intergenic	30.0%
!	AAACAGTGCTTTTGCATTTA+GGG	+	chr1.2:45697916-45697935	None:intergenic	30.0%
!	ATGCAGTTAATTCTTCCTTT+TGG	+	chr1.2:45697969-45697988	None:intergenic	30.0%
	ACCTACACTTAATGCATGTT+TGG	+	chr1.2:45698116-45698135	None:intergenic	35.0%
	ATGACATACATTAGACAACG+AGG	+	chr1.2:45697869-45697888	None:intergenic	35.0%
	GGAAGAATTAACTGCATGTT+TGG	-	chr1.2:45697972-45697991	MS.gene065713:CDS	35.0%
	TCCAAACATGCATTAAGTGT+AGG	-	chr1.2:45698112-45698131	MS.gene065713:CDS	35.0%
	TTGAAGCTTCAACCAAATGA+CGG	+	chr1.2:45698031-45698050	None:intergenic	35.0%
!!!	TGGAGTTGTTGTTTTCATGA+TGG	+	chr1.2:45697949-45697968	None:intergenic	35.0%
	AACTTAACCTATCACCACCA+AGG	-	chr1.2:45697675-45697694	MS.gene065713:intron	40.0%
	ACTGAGCTAAACCAAGAAGA+TGG	-	chr1.2:45697779-45697798	MS.gene065713:intron	40.0%
	ACTTAACCTATCACCACCAA+GGG	-	chr1.2:45697676-45697695	MS.gene065713:intron	40.0%
	ATGAGTCGAAGAAATGGAAG+TGG	-	chr1.2:45697635-45697654	MS.gene065713:CDS	40.0%
	TTCTGCATTCTTCTGCTAGT+AGG	-	chr1.2:45698150-45698169	MS.gene065713:CDS	40.0%
!	ACTGTGTGCTACCGTAATTT+TGG	+	chr1.2:45698067-45698086	None:intergenic	40.0%
!	ATCTTCTTGGTTTAGCTCAG+TGG	+	chr1.2:45697780-45697799	None:intergenic	40.0%
!!	CTTTTGCATTTAGGGCACTT+TGG	+	chr1.2:45697908-45697927	None:intergenic	40.0%
	AAAACCATGGAAGTGGCTTC+TGG	+	chr1.2:45697836-45697855	None:intergenic	45.0%
	AAACCATGGAAGTGGCTTCT+GGG	+	chr1.2:45697835-45697854	None:intergenic	45.0%
	AAGAATGCAGAACTGGCAAC+AGG	+	chr1.2:45698142-45698161	None:intergenic	45.0%
	ACCAACCAAAACCATGGAAG+TGG	+	chr1.2:45697843-45697862	None:intergenic	45.0%
	AGAATGCAGAACTGGCAACA+GGG	+	chr1.2:45698141-45698160	None:intergenic	45.0%
	AGGACAACCAACCAAAACCA+TGG	+	chr1.2:45697849-45697868	None:intergenic	45.0%
	ATCACAGTAGTGCCGTCATT+TGG	-	chr1.2:45698016-45698035	MS.gene065713:CDS	45.0%
	CTAGCAGAAGAATGCAGAAC+TGG	+	chr1.2:45698149-45698168	None:intergenic	45.0%
	GACACTGTTGCTGATCTTGT+TGG	+	chr1.2:45697728-45697747	None:intergenic	45.0%
	GTGATGACTCAACTCTTCTG+TGG	+	chr1.2:45697706-45697725	None:intergenic	45.0%
!	AGAAGCCACTTCCATGGTTT+TGG	-	chr1.2:45697835-45697854	MS.gene065713:intron	45.0%
!	GCTGGTTGTTTCCATCTTCT+TGG	+	chr1.2:45697793-45697812	None:intergenic	45.0%
!	TGTTAGAGTATCTCTGCTGC+TGG	+	chr1.2:45697811-45697830	None:intergenic	45.0%
	ACTCTTCTGTGGTCAACCCT+TGG	+	chr1.2:45697695-45697714	None:intergenic	50.0%
	TCAGTGGACACACATGAGCT+TGG	+	chr1.2:45697764-45697783	None:intergenic	50.0%
	TGGACACACATGAGCTTGGT+GGG	+	chr1.2:45697760-45697779	None:intergenic	50.0%
!!!	GCCACTTCCATGGTTTTGGT+TGG	-	chr1.2:45697839-45697858	MS.gene065713:intron	50.0%
	CTTCTGTGGTCAACCCTTGG+TGG	+	chr1.2:45697692-45697711	None:intergenic	55.0%
	GTGGACACACATGAGCTTGG+TGG	+	chr1.2:45697761-45697780	None:intergenic	55.0%
!	CATGAGCTTGGTGGGGATGT+AGG	+	chr1.2:45697752-45697771	None:intergenic	55.0%
!	GGACACACATGAGCTTGGTG+GGG	+	chr1.2:45697759-45697778	None:intergenic	55.0%
!!	GGTCAACCCTTGGTGGTGAT+AGG	+	chr1.2:45697685-45697704	None:intergenic	55.0%
	CAGCCCAGAAGCCACTTCCA+TGG	-	chr1.2:45697829-45697848	MS.gene065713:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	45697635	45698188	45697635	ID=MS.gene065713
chr1.2	mRNA	45697635	45698188	45697635	ID=MS.gene065713.t1;Parent=MS.gene065713
chr1.2	exon	45697944	45698188	45697944	ID=MS.gene065713.t1.exon1;Parent=MS.gene065713.t1
chr1.2	CDS	45697944	45698188	45697944	ID=cds.MS.gene065713.t1;Parent=MS.gene065713.t1
chr1.2	exon	45697635	45697689	45697635	ID=MS.gene065713.t1.exon2;Parent=MS.gene065713.t1
chr1.2	CDS	45697635	45697689	45697635	ID=cds.MS.gene065713.t1;Parent=MS.gene065713.t1

Gene Sequence

>MS.gene065713

ATGAGTCGAAGAAATGGAAGTGGTCCAAAACTTGACTTGAAACTTAACCTATCACCACCAAGGGTTGACCACAGAAGAGTTGAGTCATCACCAACAAGATCAGCAACAGTGTCACCTACATCCCCACCAAGCTCATGTGTGTCCACTGAGCTAAACCAAGAAGATGGAAACAACCAGCAGCAGAGATACTCTAACAGCCCAGAAGCCACTTCCATGGTTTTGGTTGGTTGTCCTCGTTGTCTAATGTATGTCATGCTTTCTGAAGATGATCCAAAGTGCCCTAAATGCAAAAGCACTGTTTTACTTGATTTCCATCATGAAAACAACAACTCCAAAAGGAAGAATTAACTGCATGTTTGGAATTACAGTGAGTTTAACGAAATCACAGTAGTGCCGTCATTTGGTTGAAGCTTCAAAATGTAGTTTTTGCCAAAATTACGGTAGCACACAGTGATTATAAAAAAAACTCACAGTCAATCCAAACATGCATTAAGTGTAGGTTACCCTGTTGCCAGTTCTGCATTCTTCTGCTAGTAGGACATGTTAGCTTTTAA

Protein sequence

>MS.gene065713.t1

MSRRNGSGPKLDLKLNLSPPRVDHRRVESSPTRSATVSPTSPPSSCVSTELNQEDGNNQQQRYSNSPEATSMVLVGCPRCLMLPCCQFCILLLVGHVSF