AlfalfaGEDB | MS.gene066897

M. sativa cultivar XinJiangDaYe / MS.gene066897

KEGG: -
GO: -
PFAM: PF00656.25

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066897.t1	XP_013445304.1	98.1	412	8	0	1	412	1	412	2.90E-228	800.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066897.t1	O64517	65.6	424	128	6	1	412	1	418	1.9e-151	537.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066897.t1	A0A072U0M2	98.1	412	8	0	1	412	1	412	2.1e-228	800.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene066897	0.831942	1.25E-55	-1.69E-46
MS.gene049807	MS.gene066897	0.843603	1.27E-58	-1.69E-46
MS.gene049887	MS.gene066897	0.843188	1.63E-58	-1.69E-46
MS.gene050224	MS.gene066897	0.839101	1.94E-57	-1.69E-46
MS.gene050359	MS.gene066897	0.84948	3.15E-60	-1.69E-46
MS.gene050414	MS.gene066897	0.811283	7.56E-51	-1.69E-46
MS.gene050646	MS.gene066897	0.818507	1.89E-52	-1.69E-46
MS.gene050689	MS.gene066897	0.802321	5.93E-49	-1.69E-46
MS.gene050838	MS.gene066897	0.80855	2.93E-50	-1.69E-46
MS.gene051003	MS.gene066897	0.813764	2.17E-51	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066897.t1	MTR_8g447080	98.058	412	8	0	1	412	1	412	0.0	833
MS.gene066897.t1	MTR_2g063570	65.865	416	120	6	1	412	4	401	0.0	538
MS.gene066897.t1	MTR_7g088035	53.548	155	68	2	5	156	9	162	7.29e-49	169
MS.gene066897.t1	MTR_7g088035	51.807	83	40	0	329	411	231	313	3.77e-22	96.7
MS.gene066897.t1	MTR_0340s0030	32.353	170	99	2	3	158	4	171	7.26e-24	100
MS.gene066897.t1	MTR_7g451400	38.000	150	86	4	3	146	74	222	2.00e-23	100
MS.gene066897.t1	MTR_2g008990	37.584	149	88	3	3	146	116	264	2.13e-20	92.8
MS.gene066897.t1	MTR_2g008990	37.584	149	88	3	3	146	116	264	2.45e-20	92.8
MS.gene066897.t1	MTR_2g008990	37.584	149	88	3	3	146	116	264	2.49e-20	92.8
MS.gene066897.t1	MTR_4g094202	36.184	152	88	4	2	146	100	249	6.08e-19	88.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066897.t1	AT1G79340	66.745	424	123	6	1	412	1	418	0.0	557
MS.gene066897.t1	AT1G79330	65.952	420	125	6	1	412	1	410	0.0	550
MS.gene066897.t1	AT1G79310	55.924	422	157	9	1	412	1	403	1.28e-158	453
MS.gene066897.t1	AT1G79320	57.488	414	128	6	1	412	1	368	5.36e-158	450
MS.gene066897.t1	AT1G16420	41.801	433	178	10	1	412	1	380	2.62e-98	298
MS.gene066897.t1	AT5G04200	36.516	419	156	12	1	411	6	322	1.40e-72	230
MS.gene066897.t1	AT1G02170	38.255	149	86	4	3	146	80	227	1.32e-23	101
MS.gene066897.t1	AT4G25110	37.162	148	88	3	3	146	116	262	1.21e-22	99.4
MS.gene066897.t1	AT4G25110	36.913	149	88	4	3	146	116	263	1.03e-21	96.7
MS.gene066897.t1	AT5G64240	34.228	149	92	3	3	146	90	237	2.54e-18	86.3

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Find 115 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATATCAGCACAAGAA+AGG	0.502276	8.4:+54603843	None:intergenic
AAAGCCTGATAATCCAAAGC+CGG	0.593904	8.4:+54604574	None:intergenic
AAAGCTGAATGATAATGATG+AGG	0.590743	8.4:-54604183	MS.gene066897:CDS
AAATGCTTGATCGAACGTTA+CGG	0.540516	8.4:-54605558	MS.gene066897:CDS
AACAAGCTCCAGCATGGAGG+TGG	0.540605	8.4:-54604269	MS.gene066897:CDS
AAGACGTGTTCTGTCGGAGC+TGG	0.553482	8.4:-54605463	MS.gene066897:CDS
AAGCGCGGTCTTCCAAGCGG+TGG	0.560038	8.4:-54604095	MS.gene066897:CDS
AAGCTGAATGATAATGATGA+GGG	0.577946	8.4:-54604182	MS.gene066897:CDS
AAGGGAGAAGAGGAACATTC+TGG	0.411416	8.4:-54604599	MS.gene066897:CDS
ACAAGAAAGGATCATCAACA+TGG	0.709272	8.4:+54603856	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTCAAGGAATTTGTGGAT+GGG	-	chr8.4:54604784-54604803	MS.gene066897:intron	30.0%
CATGAATATAAAGGAACAGA+AGG	-	chr8.4:54604608-54604627	MS.gene066897:CDS	30.0%
CTGCTTAAGTATATCAATGA+GGG	+	chr8.4:54605102-54605121	None:intergenic	30.0%
GATATACTTAAGCAGAAAAC+TGG	-	chr8.4:54605107-54605126	MS.gene066897:intron	30.0%
GGGAAAGAAAATATAGATGT+TGG	-	chr8.4:54605128-54605147	MS.gene066897:intron	30.0%
TCTGCTTAAGTATATCAATG+AGG	+	chr8.4:54605103-54605122	None:intergenic	30.0%
TGATGATTTCAAGGAATTTG+TGG	-	chr8.4:54604779-54604798	MS.gene066897:intron	30.0%
AAATGCTTGATCGAACGTTA+CGG	-	chr8.4:54603941-54603960	MS.gene066897:CDS	35.0%
ACAAGAAAGGATCATCAACA+TGG	+	chr8.4:54605646-54605665	None:intergenic	35.0%
AGTAAGCATGAAGTATATGC+TGG	-	chr8.4:54605374-54605393	MS.gene066897:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	54603851	54605670	54603851	ID=MS.gene066897
chr8.4	mRNA	54603851	54605670	54603851	ID=MS.gene066897.t1;Parent=MS.gene066897
chr8.4	exon	54605325	54605670	54605325	ID=MS.gene066897.t1.exon1;Parent=MS.gene066897.t1
chr8.4	CDS	54605325	54605670	54605325	ID=cds.MS.gene066897.t1;Parent=MS.gene066897.t1
chr8.4	exon	54603851	54604743	54603851	ID=MS.gene066897.t1.exon2;Parent=MS.gene066897.t1
chr8.4	CDS	54603851	54604743	54603851	ID=cds.MS.gene066897.t1;Parent=MS.gene066897.t1

Gene Sequence

>MS.gene066897

ATGACGAAAAAAGCTGTATTGATCGGAATCAACTACCCAGGAACAAAAGCAGAGTTACGAGGATGCGTAAACGACGTGTGGAGGATGCACAAATGCTTGATCGAACGTTACGGTTTTTCCGAAGATGACATCACTGTTTTGATCGACACGGATTCGTCGTACACGGAGCCGACAGGAAAGAACATAAGACGTGTTCTGTCGGAGCTGGTCAGATCTTCGGAGGACGGTGATCTGCTGTTTGTTCATTATAGTGGACATGGGACTCGTCTTCCTGCTGAAACCGGAGAGGATGATGATACTGGTTTTGATGAATGTATTGTTCCTTCTGATATGAATCTCATCACTGGTTCGTACCAAGAAAAAGAAAAAACTACTCTCCTTTTTATTTACCTTTTTCTCTCTGTTTTTATTTTTGTTTTGATTAAGTGAATTGTATGTTAATTTAAATGTTTAATTTTGTGATTTTGGATCCTGTGGTTACAGCTGAAAGTTGTTTTAAAATTATTATTACTTTTTGACAAAAAGTGTTTTAAGAATTGAGAAACGATTTCAACGTGTTTGATTTGATTTGATTTGATTTGATGATAAGGAATCTCCAGGGACGGAATTCTCTACAAGAAACTTTTTAAAGTTTTTACTTTATTTGAAAATTCATAGATCAGTTAAGATTGTCTCCATAGTGGAATTGACCGGAATCTCACAACCTCCGGAACCAATGTTCGAATTTTAATTGAGAAATGTGCATACATTTAGTATACATGAATATAAAGGAACAGAAGGGAACGTGTGGAAAAAATGTATTCATAGCTTGTGATTCATGATTTCTTTGTAGTCTCGTGCATGTCTGGAATCTTTATATAGGGTAATTCTGATGTTATTAGTGAGTAGTAATGGTAGTAGTATTGCTGTCTTTTTGTGTGTGAACAGATGATGATTTCAAGGAATTTGTGGATGGGGTTCCTAGAGGATGTAGGCTGACCATTGTATCTGATTCATGCCACAGTGGTGGCTTGATTGAAGAAGCTAAGGAGCAGATAGGAGAGAGCACAAAGGGAGAAGAGGAACATTCTGGATCCGGCTTTGGATTATCAGGCTTTCTGCATAGGAAAGTTGAGGATGCTATTGAGTCCAGGGGATTTCATGTCCCTTCAGGATTGCGTCGTCATACACACAGGGATGAAGAAACAAATGAAGATAGGGATATCGAACTCCCACATGGAGTGAAGAATAGGTCTCTGCCACTTTCAACCCTCATTGATATACTTAAGCAGAAAACTGGGAAAGAAAATATAGATGTTGGTCAACTGAGGCCTACACTTTTTGACGTTTTTGGGGAAGATGCTAGTCCTAAAGTGAAGAAGTTTATGAAAGTCATCTTGAACAAGCTCCAGCATGGAGGTGGTGAAGGTGGACAAGGTGGGATATTGGGAATGGTGGGTAGTCTTGCCCAAGAGTTTCTTAAGCAAAAGCTGAATGATAATGATGAGGGATATGCAAAGCCTGCCATGGAGACGGAAGTGGGAAGTAAGCATGAAGTATATGCTGGATCGTCAAAGCGCGGTCTTCCAAGCGGTGGGATTCTGATGAGTGGCTGTCAGACAGACCAGACTTCAGCAGATGCTTGTCCTGCCGGAAATGCAGCTAATGCTTATGGAGCTTTCAGCAATGCAATACAGGCTATAATTGAGGAGACTGATGGTGCAATTACATACTCGGAGCTTATTCTCAAGGCTAGAGAGAAGCTACAGAAAGATGGATTCACTCAAAAGCCAGGACTCTATTGCAGTGATCACCATGTTGATGATCCTTTCTTGTGCTGA

Protein sequence

>MS.gene066897.t1

MTKKAVLIGINYPGTKAELRGCVNDVWRMHKCLIERYGFSEDDITVLIDTDSSYTEPTGKNIRRVLSELVRSSEDGDLLFVHYSGHGTRLPAETGEDDDTGFDECIVPSDMNLITDDDFKEFVDGVPRGCRLTIVSDSCHSGGLIEEAKEQIGESTKGEEEHSGSGFGLSGFLHRKVEDAIESRGFHVPSGLRRHTHRDEETNEDRDIELPHGVKNRSLPLSTLIDILKQKTGKENIDVGQLRPTLFDVFGEDASPKVKKFMKVILNKLQHGGGEGGQGGILGMVGSLAQEFLKQKLNDNDEGYAKPAMETEVGSKHEVYAGSSKRGLPSGGILMSGCQTDQTSADACPAGNAANAYGAFSNAIQAIIEETDGAITYSELILKAREKLQKDGFTQKPGLYCSDHHVDDPFLC