AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068127


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068127.t1 MTR_8g069450 96.875 128 4 0 1 128 128 255 1.98e-88 257
MS.gene068127.t1 MTR_8g069450 96.875 128 4 0 1 128 128 255 2.43e-88 257
MS.gene068127.t1 MTR_8g069450 96.875 128 4 0 1 128 128 255 4.42e-87 256
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068127.t1 AT3G26580 81.250 128 24 0 1 128 154 281 2.00e-75 228

Find 39 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACCAAGATCTACATTATT+TGG 0.191918 8.1:+35791587 MS.gene068127:CDS
GCAATCCTTATGGCGATTAT+TGG 0.215703 8.1:-35791773 None:intergenic
TGCAGAACTTCGTTACATTT+TGG 0.260230 8.1:+35791855 MS.gene068127:CDS
GATTTGGGTAAGAAAGCTTA+TGG 0.281355 8.1:+35791512 MS.gene068127:CDS
GTGAATTTCAGATACAAATA+TGG 0.289294 8.1:+35791731 MS.gene068127:intron
AGCTCAGTTGATGTATGATT+TGG 0.292561 8.1:+35791496 MS.gene068127:CDS
AAGAGAAGAGGATGAGAGTT+AGG 0.351749 8.1:+35790237 None:intergenic
GTATAGTCACCATCTCTTCT+TGG 0.352541 8.1:-35791900 None:intergenic
TCTTGGGTTATCTTGAGCTT+TGG 0.386466 8.1:-35791883 None:intergenic
TATAGTCACCATCTCTTCTT+GGG 0.408773 8.1:-35791899 None:intergenic
TGACGTTTGATGCTAATACT+AGG 0.431805 8.1:-35791829 None:intergenic
GCTCAGTTGATGTATGATTT+GGG 0.432267 8.1:+35791497 MS.gene068127:CDS
AAAGAGCAATGCAATCCTTA+TGG 0.434951 8.1:-35791783 None:intergenic
CCACCAAATAATGTAGATCT+TGG 0.439479 8.1:-35791590 None:intergenic
AGTTAGGAAAGAGCTTGAAA+AGG 0.442833 8.1:+35790253 MS.gene068127:CDS
ATTTGGGTAAGAAAGCTTAT+GGG 0.445943 8.1:+35791513 MS.gene068127:CDS
ATGGCTGGCTATGGCTTATG+AGG 0.453962 8.1:+35791750 MS.gene068127:CDS
GTCATAACTAGAACCAATCA+AGG 0.468929 8.1:-35791923 None:intergenic
ATGGTGACTATACCCTTGAT+TGG 0.471938 8.1:+35791910 MS.gene068127:CDS
CGTGCCGTTGAGTTTCTTGA+AGG 0.493680 8.1:+35791551 MS.gene068127:CDS
GACGTTTGATGCTAATACTA+GGG 0.495643 8.1:-35791828 None:intergenic
ATCTACATTATTTGGTGGTG+AGG 0.501114 8.1:+35791595 MS.gene068127:CDS
TGATTGGTTCTAGTTATGAC+AGG 0.513314 8.1:+35791926 MS.gene068127:CDS
GAATCAGGTGGCTAAGGAGC+AGG 0.526095 8.1:+35791457 MS.gene068127:intron
CCAAGATCTACATTATTTGG+TGG 0.527272 8.1:+35791590 MS.gene068127:CDS
GCTTATGGGAGAGGCACGTA+TGG 0.534948 8.1:+35791527 MS.gene068127:CDS
TAAGGAGCAGGCAGAGAGGA+GGG 0.539752 8.1:+35791469 MS.gene068127:CDS
GGTAAGAAAGCTTATGGGAG+AGG 0.539832 8.1:+35791518 MS.gene068127:CDS
TGAGGCCAATAATCGCCATA+AGG 0.547038 8.1:+35791768 MS.gene068127:CDS
TAATGTAGATCTTGGTATGA+TGG 0.552370 8.1:-35791582 None:intergenic
ATTTCAGATACAAATATGGC+TGG 0.553752 8.1:+35791735 MS.gene068127:intron
GATACAAATATGGCTGGCTA+TGG 0.569208 8.1:+35791741 MS.gene068127:intron
CAAGATAACCCAAGAAGAGA+TGG 0.579385 8.1:+35791891 MS.gene068127:CDS
CTAAGGAGCAGGCAGAGAGG+AGG 0.584864 8.1:+35791468 MS.gene068127:CDS
TTTGACGAATCAGGTGGCTA+AGG 0.599329 8.1:+35791451 MS.gene068127:intron
TGCACCTTCAAGAAACTCAA+CGG 0.650398 8.1:-35791555 None:intergenic
ATCATACATCAACTGAGCTG+TGG 0.650968 8.1:-35791492 None:intergenic
TCATAACTAGAACCAATCAA+GGG 0.666833 8.1:-35791922 None:intergenic
TGGCTAAGGAGCAGGCAGAG+AGG 0.675876 8.1:+35791465 MS.gene068127:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGCAATTTTTTATTTTTT+AGG + chr8.1:35790765-35790784 MS.gene068127:intron 10.0%
!!! AATTTTGTAGTTTTTGTTTT+TGG + chr8.1:35790403-35790422 MS.gene068127:intron 15.0%
!! CATATACATCATTTATTCAA+CGG + chr8.1:35790827-35790846 MS.gene068127:intron 20.0%
!! GATGTATATGATCTATATTA+TGG - chr8.1:35790817-35790836 None:intergenic 20.0%
!! TAAACAGTAGTTTAGTATTT+AGG - chr8.1:35790434-35790453 None:intergenic 20.0%
!! TAACAAGGATAATTTCTTAA+AGG + chr8.1:35790452-35790471 MS.gene068127:intron 20.0%
!! TTGCTTACAAATATTTGTTT+AGG - chr8.1:35791343-35791362 None:intergenic 20.0%
!!! AATAACTATTCTTGGTTTTT+TGG + chr8.1:35791164-35791183 MS.gene068127:intron 20.0%
!!! AGATGGATTGTAATATTATA+TGG + chr8.1:35791136-35791155 MS.gene068127:intron 20.0%
!!! GATTCTTATTTAGTTAAGAA+TGG - chr8.1:35791626-35791645 None:intergenic 20.0%
!!! TTTTTGACTAATTTGTTGAT+TGG + chr8.1:35790924-35790943 MS.gene068127:intron 20.0%
! ACAGTTATGAGAAAATCATT+TGG - chr8.1:35790512-35790531 None:intergenic 25.0%
! ATACCAAGATCTACATTATT+TGG + chr8.1:35791587-35791606 MS.gene068127:CDS 25.0%
! CAGTTATGAGAAAATCATTT+GGG - chr8.1:35790511-35790530 None:intergenic 25.0%
! CATTGAATAAATGATGTATG+TGG + chr8.1:35790790-35790809 MS.gene068127:intron 25.0%
! GTGAATTTCAGATACAAATA+TGG + chr8.1:35791731-35791750 MS.gene068127:intron 25.0%
! GTGTATGTAAAGAAAACAAA+AGG - chr8.1:35791025-35791044 None:intergenic 25.0%
! TACTAAACTACTGTTTAACA+AGG + chr8.1:35790437-35790456 MS.gene068127:intron 25.0%
!!! GTTTTCATACTTTTTGTTTG+AGG + chr8.1:35791219-35791238 MS.gene068127:intron 25.0%
!!! TGTAGTTTTTGTTTTTGGAA+AGG + chr8.1:35790408-35790427 MS.gene068127:intron 25.0%
AACTATTACATATGAGACAG+AGG - chr8.1:35790358-35790377 None:intergenic 30.0%
ATTTCAGATACAAATATGGC+TGG + chr8.1:35791735-35791754 MS.gene068127:intron 30.0%
ATTTGGGTAAGAAAGCTTAT+GGG + chr8.1:35791513-35791532 MS.gene068127:CDS 30.0%
CTAGAAGTGAAACATTAAGA+TGG + chr8.1:35791119-35791138 MS.gene068127:intron 30.0%
TAATGTAGATCTTGGTATGA+TGG - chr8.1:35791585-35791604 None:intergenic 30.0%
TCATAACTAGAACCAATCAA+GGG - chr8.1:35791925-35791944 None:intergenic 30.0%
TGCTTATAATTGAGACTAGA+GGG - chr8.1:35790751-35790770 None:intergenic 30.0%
TGGTGCATAATAACTATTCT+TGG + chr8.1:35791156-35791175 MS.gene068127:intron 30.0%
TGTCAGTTGTCTAATGATAA+AGG - chr8.1:35791080-35791099 None:intergenic 30.0%
TTGCTTATAATTGAGACTAG+AGG - chr8.1:35790752-35790771 None:intergenic 30.0%
! ATCTTTTTGTTGGGACTTAT+GGG - chr8.1:35790706-35790725 None:intergenic 30.0%
AAAACCCGAAAGCAGTTATA+TGG - chr8.1:35790602-35790621 None:intergenic 35.0%
AAAGAGCAATGCAATCCTTA+TGG - chr8.1:35791786-35791805 None:intergenic 35.0%
ACAAAGTTTCACGATAACAG+TGG - chr8.1:35790994-35791013 None:intergenic 35.0%
ACTCGTTGACATATTGTTTG+TGG + chr8.1:35791428-35791447 MS.gene068127:intron 35.0%
ACTGTTATCGTGAAACTTTG+TGG + chr8.1:35790993-35791012 MS.gene068127:intron 35.0%
AGTTAGGAAAGAGCTTGAAA+AGG + chr8.1:35790253-35790272 MS.gene068127:CDS 35.0%
CAAAGTTTCACGATAACAGT+GGG - chr8.1:35790993-35791012 None:intergenic 35.0%
CACAAGTATGTCTAGATACT+AGG - chr8.1:35790634-35790653 None:intergenic 35.0%
CCAAGATCTACATTATTTGG+TGG + chr8.1:35791590-35791609 MS.gene068127:CDS 35.0%
CCACCAAATAATGTAGATCT+TGG - chr8.1:35791593-35791612 None:intergenic 35.0%
GACGTTTGATGCTAATACTA+GGG - chr8.1:35791831-35791850 None:intergenic 35.0%
GATTTGGGTAAGAAAGCTTA+TGG + chr8.1:35791512-35791531 MS.gene068127:CDS 35.0%
GTCATAACTAGAACCAATCA+AGG - chr8.1:35791926-35791945 None:intergenic 35.0%
TATAGTCACCATCTCTTCTT+GGG - chr8.1:35791902-35791921 None:intergenic 35.0%
TGACGTTTGATGCTAATACT+AGG - chr8.1:35791832-35791851 None:intergenic 35.0%
! ACTCTCTCGATCTTTTTGTT+GGG - chr8.1:35790715-35790734 None:intergenic 35.0%
! AGCTCAGTTGATGTATGATT+TGG + chr8.1:35791496-35791515 MS.gene068127:CDS 35.0%
! GATCTTTTTGTTGGGACTTA+TGG - chr8.1:35790707-35790726 None:intergenic 35.0%
! GCTCAGTTGATGTATGATTT+GGG + chr8.1:35791497-35791516 MS.gene068127:CDS 35.0%
! TGATCCTCTCTAGTGTTTTA+AGG + chr8.1:35790677-35790696 MS.gene068127:intron 35.0%
! TGCAGAACTTCGTTACATTT+TGG + chr8.1:35791855-35791874 MS.gene068127:CDS 35.0%
!! ACCATTATTTTTCTCCTCTC+GGG + chr8.1:35791382-35791401 MS.gene068127:intron 35.0%
!! AGACAGAGGAGTACTAATTA+AGG - chr8.1:35790344-35790363 None:intergenic 35.0%
!! ATCTACATTATTTGGTGGTG+AGG + chr8.1:35791595-35791614 MS.gene068127:CDS 35.0%
!! GACCATTATTTTTCTCCTCT+CGG + chr8.1:35791381-35791400 MS.gene068127:intron 35.0%
!! TTCACTTCTAGTGACTTGTT+AGG - chr8.1:35791110-35791129 None:intergenic 35.0%
!!! ATATAACTGCTTTCGGGTTT+TGG + chr8.1:35790601-35790620 MS.gene068127:intron 35.0%
!!! TATAACTGCTTTCGGGTTTT+GGG + chr8.1:35790602-35790621 MS.gene068127:intron 35.0%
ACCCGAGAGGAGAAAAATAA+TGG - chr8.1:35791386-35791405 None:intergenic 40.0%
ATCATACATCAACTGAGCTG+TGG - chr8.1:35791495-35791514 None:intergenic 40.0%
ATGGTGACTATACCCTTGAT+TGG + chr8.1:35791910-35791929 MS.gene068127:CDS 40.0%
CAAGATAACCCAAGAAGAGA+TGG + chr8.1:35791891-35791910 MS.gene068127:CDS 40.0%
GAAGCCTTAAAACACTAGAG+AGG - chr8.1:35790684-35790703 None:intergenic 40.0%
GCAATCCTTATGGCGATTAT+TGG - chr8.1:35791776-35791795 None:intergenic 40.0%
GTATAGTCACCATCTCTTCT+TGG - chr8.1:35791903-35791922 None:intergenic 40.0%
GTGGAGGGTGATGATTAAAT+TGG + chr8.1:35791404-35791423 MS.gene068127:intron 40.0%
TCTTGGGTTATCTTGAGCTT+TGG - chr8.1:35791886-35791905 None:intergenic 40.0%
TGCACCTTCAAGAAACTCAA+CGG - chr8.1:35791558-35791577 None:intergenic 40.0%
TGTTTGTGGTTTGACGAATC+AGG + chr8.1:35791442-35791461 MS.gene068127:intron 40.0%
! CACTCTCTCGATCTTTTTGT+TGG - chr8.1:35790716-35790735 None:intergenic 40.0%
! GATACAAATATGGCTGGCTA+TGG + chr8.1:35791741-35791760 MS.gene068127:intron 40.0%
!! ATTATTTTTCTCCTCTCGGG+TGG + chr8.1:35791385-35791404 MS.gene068127:intron 40.0%
CCAACGAGACATCGCTTAAT+TGG - chr8.1:35791047-35791066 None:intergenic 45.0%
CCAATTAAGCGATGTCTCGT+TGG + chr8.1:35791044-35791063 MS.gene068127:intron 45.0%
CCCTGCCATATAACTGCTTT+CGG + chr8.1:35790594-35790613 MS.gene068127:intron 45.0%
CCGAAAGCAGTTATATGGCA+GGG - chr8.1:35790597-35790616 None:intergenic 45.0%
CCTGCCATATAACTGCTTTC+GGG + chr8.1:35790595-35790614 MS.gene068127:intron 45.0%
GGTAAGAAAGCTTATGGGAG+AGG + chr8.1:35791518-35791537 MS.gene068127:CDS 45.0%
TGAGGCCAATAATCGCCATA+AGG + chr8.1:35791768-35791787 MS.gene068127:CDS 45.0%
TTGTGGTTTGACGAATCAGG+TGG + chr8.1:35791445-35791464 MS.gene068127:intron 45.0%
TTTGACGAATCAGGTGGCTA+AGG + chr8.1:35791451-35791470 MS.gene068127:intron 45.0%
CCCGAAAGCAGTTATATGGC+AGG - chr8.1:35790598-35790617 None:intergenic 50.0%
! ATGGCTGGCTATGGCTTATG+AGG + chr8.1:35791750-35791769 MS.gene068127:CDS 50.0%
! ATTTTTCTCCTCTCGGGTGG+AGG + chr8.1:35791388-35791407 MS.gene068127:intron 50.0%
! CGTGCCGTTGAGTTTCTTGA+AGG + chr8.1:35791551-35791570 MS.gene068127:CDS 50.0%
! TTTTTCTCCTCTCGGGTGGA+GGG + chr8.1:35791389-35791408 MS.gene068127:intron 50.0%
GAATCAGGTGGCTAAGGAGC+AGG + chr8.1:35791457-35791476 MS.gene068127:intron 55.0%
TAAGGAGCAGGCAGAGAGGA+GGG + chr8.1:35791469-35791488 MS.gene068127:CDS 55.0%
! GCTTATGGGAGAGGCACGTA+TGG + chr8.1:35791527-35791546 MS.gene068127:CDS 55.0%
ATCATCACCCTCCACCCGAG+AGG - chr8.1:35791399-35791418 None:intergenic 60.0%
CTAAGGAGCAGGCAGAGAGG+AGG + chr8.1:35791468-35791487 MS.gene068127:CDS 60.0%
TGGCTAAGGAGCAGGCAGAG+AGG + chr8.1:35791465-35791484 MS.gene068127:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 35790248 35791947 35790248 ID=MS.gene068127
chr8.1 mRNA 35790248 35791947 35790248 ID=MS.gene068127.t1;Parent=MS.gene068127
chr8.1 exon 35790248 35790274 35790248 ID=MS.gene068127.t1.exon1;Parent=MS.gene068127.t1
chr8.1 CDS 35790248 35790274 35790248 ID=cds.MS.gene068127.t1;Parent=MS.gene068127.t1
chr8.1 exon 35791464 35791616 35791464 ID=MS.gene068127.t1.exon2;Parent=MS.gene068127.t1
chr8.1 CDS 35791464 35791616 35791464 ID=cds.MS.gene068127.t1;Parent=MS.gene068127.t1
chr8.1 exon 35791742 35791947 35791742 ID=MS.gene068127.t1.exon3;Parent=MS.gene068127.t1
chr8.1 CDS 35791742 35791947 35791742 ID=cds.MS.gene068127.t1;Parent=MS.gene068127.t1
Gene Sequence

>MS.gene068127

ATGAGAGTTAGGAAAGAGCTTGAAAAGGTTTGAGTTAATTAATTAAACTAATTATTATTACTTCTATAATGATATGATTTTCTTGTCTTCTTTCCTTAATTAGTACTCCTCTGTCTCATATGTAATAGTTTGAAATATATCAATTAATATATCTCAATTTTGTAGTTTTTGTTTTTGGAAAGGCCTAAATACTAAACTACTGTTTAACAAGGATAATTTCTTAAAGGAAGTATATTTTATCATAGAAATTAGTATATTAACCCAAATGATTTTCTCATAACTGTGTATCATAAGTTTGTTGATGTGTTAAAGAGTTAAAATTTACTACTAAAAAATAGATGTTGAGCCCTGCCATATAACTGCTTTCGGGTTTTGGGCGATGACCTAGTATCTAGACATACTTGTGTAATTGTTCTTACTCTAATGTGATGATCCTCTCTAGTGTTTTAAGGCTTCCCATAAGTCCCAACAAAAAGATCGAGAGAGTGTATGTTTGTACTCCCTCTAGTCTCAATTATAAGCAATTTTTTATTTTTTAGGTTCATTGAATAAATGATGTATGTGGTCCATAATATAGATCATATACATCATTTATTCAACGGACTTAAAAAATGAAAAGTTGCTTATAATTGAGACAAGAAGAAGTATGTTTTTAATATTTTAATATTTGCTATTTTTTTTGACTAATTTGTTGATTGGTTATGTTTAGAAAGAATTCTTGCACGCACTCATTGTTGTTGTACCCACTGTTATCGTGAAACTTTGTGGTTATTTCCTTTTGTTTTCTTTACATACACCAATTAAGCGATGTCTCGTTGGTGTTTGTCAACCTTTATCATTAGACAACTGACACAATTGTCCTAACAAGTCACTAGAAGTGAAACATTAAGATGGATTGTAATATTATATGGTGCATAATAACTATTCTTGGTTTTTTGGTTTGAGAAAATGTTTTCTATTTTTATTATTTTGTTTTCATACTTTTTGTTTGAGGAGTTATGTTTTCATTCTTAATTATACTTTATGTTTTCAATCTTATTTACTTAAAACTAAACTACTACTTATTTTCAATACATATTGTTTTCATAGTTTCCTAAACAAATATTTGTAAGCAAAACTGAAGTTGTTGAGATGACCATTATTTTTCTCCTCTCGGGTGGAGGGTGATGATTAAATTGGCACTCGTTGACATATTGTTTGTGGTTTGACGAATCAGGTGGCTAAGGAGCAGGCAGAGAGGAGGGCCACAGCTCAGTTGATGTATGATTTGGGTAAGAAAGCTTATGGGAGAGGCACGTATGGACGTGCCGTTGAGTTTCTTGAAGGTGCACTCACCATCATACCAAGATCTACATTATTTGGTGGTGAGGTTAGTCCATTCTTAACTAAATAAGAATCTCTACATTTTCTTTCTTTGATTTATTTTCATTTGTCTTTAATTGATTAAAGAGCAAGTAAAAGAAGTTTTTTTATTAAGAAAATTGTGAATTTCAGATACAAATATGGCTGGCTATGGCTTATGAGGCCAATAATCGCCATAAGGATTGCATTGCTCTTTACAAGCAATTAGAAAAGTCGCACCCTAGTATTAGCATCAAACGTCAAGCTGCAGAACTTCGTTACATTTTGGAAGCACCAAAGCTCAAGATAACCCAAGAAGAGATGGTGACTATACCCTTGATTGGTTCTAGTTATGACAG

Protein sequence

>MS.gene068127.t1

MRVRKELEKVAKEQAERRATAQLMYDLGKKAYGRGTYGRAVEFLEGALTIIPRSTLFGGEIQIWLAMAYEANNRHKDCIALYKQLEKSHPSISIKRQAAELRYILEAPKLKITQEEMVTIPLIGSSYD