AlfalfaGEDB | MS.gene069870

M. sativa cultivar XinJiangDaYe / MS.gene069870

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069870.t1	XP_003597686.1	98.5	197	2	1	1	197	1	196	1.10E-103	386

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069870.t1	G7II08	98.5	197	2	1	1	197	1	196	7.8e-104	386.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene069870	0.846503	2.09E-59	-1.69E-46
MS.gene049369	MS.gene069870	0.82848	8.78E-55	-1.69E-46
MS.gene050128	MS.gene069870	0.80212	6.52E-49	-1.69E-46
MS.gene050301	MS.gene069870	0.805116	1.56E-49	-1.69E-46
MS.gene050398	MS.gene069870	0.800384	1.48E-48	-1.69E-46
MS.gene050670	MS.gene069870	0.845621	3.62E-59	-1.69E-46
MS.gene050971	MS.gene069870	0.827924	1.20E-54	-1.69E-46
MS.gene05105	MS.gene069870	0.808351	3.23E-50	-1.69E-46
MS.gene052188	MS.gene069870	0.85172	7.38E-61	-1.69E-46
MS.gene052253	MS.gene069870	0.836574	8.64E-57	-1.69E-46
MS.gene052310	MS.gene069870	0.807469	4.98E-50	-1.69E-46
MS.gene052340	MS.gene069870	0.841722	4.00E-58	-1.69E-46
MS.gene052414	MS.gene069870	0.807349	5.28E-50	-1.69E-46
MS.gene052421	MS.gene069870	0.802927	4.45E-49	-1.69E-46
MS.gene052485	MS.gene069870	0.817648	2.96E-52	-1.69E-46
MS.gene05316	MS.gene069870	0.845458	4.01E-59	-1.69E-46
MS.gene053449	MS.gene069870	0.816593	5.10E-52	-1.69E-46
MS.gene053758	MS.gene069870	0.8382	3.31E-57	-1.69E-46
MS.gene053813	MS.gene069870	0.835141	1.99E-56	-1.69E-46
MS.gene054123	MS.gene069870	0.808191	3.50E-50	-1.69E-46
MS.gene054183	MS.gene069870	0.807958	3.92E-50	-1.69E-46
MS.gene054349	MS.gene069870	0.838162	3.39E-57	-1.69E-46
MS.gene054556	MS.gene069870	0.807887	4.06E-50	-1.69E-46
MS.gene055116	MS.gene069870	0.859195	4.89E-63	-1.69E-46
MS.gene055369	MS.gene069870	0.811126	8.18E-51	-1.69E-46
MS.gene055909	MS.gene069870	0.82986	4.06E-55	-1.69E-46
MS.gene055911	MS.gene069870	0.835786	1.37E-56	-1.69E-46
MS.gene056191	MS.gene069870	0.814298	1.65E-51	-1.69E-46
MS.gene056407	MS.gene069870	0.809929	1.48E-50	-1.69E-46
MS.gene056511	MS.gene069870	0.850249	1.92E-60	-1.69E-46
MS.gene056723	MS.gene069870	0.806441	8.22E-50	-1.69E-46
MS.gene057396	MS.gene069870	0.846831	1.70E-59	-1.69E-46
MS.gene057448	MS.gene069870	0.847413	1.18E-59	-1.69E-46
MS.gene057449	MS.gene069870	0.850485	1.65E-60	-1.69E-46
MS.gene057450	MS.gene069870	0.845096	5.02E-59	-1.69E-46
MS.gene057610	MS.gene069870	0.803266	3.78E-49	-1.69E-46
MS.gene057749	MS.gene069870	0.827234	1.75E-54	-1.69E-46
MS.gene057823	MS.gene069870	0.822443	2.36E-53	-1.69E-46
MS.gene057927	MS.gene069870	0.804273	2.34E-49	-1.69E-46
MS.gene058002	MS.gene069870	0.806279	8.89E-50	-1.69E-46
MS.gene058007	MS.gene069870	0.844262	8.43E-59	-1.69E-46
MS.gene05842	MS.gene069870	0.805503	1.29E-49	-1.69E-46
MS.gene058700	MS.gene069870	0.800911	1.15E-48	-1.69E-46
MS.gene058928	MS.gene069870	0.845172	4.79E-59	-1.69E-46
MS.gene058949	MS.gene069870	0.842163	3.06E-58	-1.69E-46
MS.gene058995	MS.gene069870	0.831752	1.40E-55	-1.69E-46
MS.gene05907	MS.gene069870	0.841731	3.98E-58	-1.69E-46
MS.gene059080	MS.gene069870	0.81421	1.73E-51	-1.69E-46
MS.gene059082	MS.gene069870	0.83144	1.67E-55	-1.69E-46
MS.gene059294	MS.gene069870	0.82209	2.85E-53	-1.69E-46
MS.gene059366	MS.gene069870	0.828072	1.10E-54	-1.69E-46
MS.gene059420	MS.gene069870	0.834281	3.28E-56	-1.69E-46
MS.gene059462	MS.gene069870	0.849898	2.41E-60	-1.69E-46
MS.gene059674	MS.gene069870	0.843407	1.43E-58	-1.69E-46
MS.gene059676	MS.gene069870	0.80017	1.63E-48	-1.69E-46
MS.gene059688	MS.gene069870	0.837848	4.08E-57	-1.69E-46
MS.gene059692	MS.gene069870	0.828392	9.22E-55	-1.69E-46
MS.gene059695	MS.gene069870	0.830933	2.22E-55	-1.69E-46
MS.gene059807	MS.gene069870	0.874805	5.07E-68	-1.69E-46
MS.gene059876	MS.gene069870	0.837253	5.80E-57	-1.69E-46
MS.gene059933	MS.gene069870	0.807046	6.12E-50	-1.69E-46
MS.gene060403	MS.gene069870	0.818346	2.06E-52	-1.69E-46
MS.gene060495	MS.gene069870	0.837677	4.51E-57	-1.69E-46
MS.gene060837	MS.gene069870	0.813414	2.59E-51	-1.69E-46
MS.gene061049	MS.gene069870	0.816255	6.08E-52	-1.69E-46
MS.gene061051	MS.gene069870	-0.81143	7.03E-51	-1.69E-46
MS.gene061154	MS.gene069870	0.821841	3.26E-53	-1.69E-46
MS.gene061159	MS.gene069870	0.808351	3.23E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069870.t1	MTR_2g101140	98.477	197	2	1	1	197	1	196	2.17e-143	397
MS.gene069870.t1	MTR_8g013990	67.500	200	54	5	1	197	3	194	6.24e-92	267
MS.gene069870.t1	MTR_8g021190	42.969	128	69	3	62	187	29	154	2.33e-29	107
MS.gene069870.t1	MTR_1g103550	47.191	89	41	2	63	149	2	86	2.88e-22	87.8
MS.gene069870.t1	MTR_5g006430	38.053	113	65	2	63	174	2	110	1.37e-20	82.8
MS.gene069870.t1	MTR_3g105340	52.778	72	33	1	53	123	1	72	2.63e-20	82.8
MS.gene069870.t1	MTR_3g109860	34.959	123	77	3	33	152	4	126	1.36e-17	76.3
MS.gene069870.t1	MTR_4g125010	50.725	69	33	1	62	129	23	91	5.83e-17	74.7
MS.gene069870.t1	MTR_4g125030	42.708	96	47	3	62	152	21	113	3.42e-16	71.6
MS.gene069870.t1	MTR_1g014930	41.111	90	39	3	54	136	4	86	2.17e-15	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069870.t1	AT5G25840	50.276	181	59	4	37	197	4	173	3.88e-53	168
MS.gene069870.t1	AT1G79770	53.205	156	59	3	36	191	26	167	1.21e-48	156
MS.gene069870.t1	AT1G72510	33.537	164	90	5	40	187	5	165	8.62e-23	90.1
MS.gene069870.t1	AT1G72510	33.537	164	90	5	40	187	5	165	8.62e-23	90.1
MS.gene069870.t1	AT4G14819	40.777	103	50	3	63	165	2	93	5.60e-22	86.3
MS.gene069870.t1	AT2G25780	35.000	160	91	4	31	186	1	151	1.90e-21	86.3
MS.gene069870.t1	AT3G22540	40.449	89	50	1	63	151	2	87	2.03e-21	85.1
MS.gene069870.t1	AT2G09970	31.707	164	92	5	41	187	6	166	1.14e-20	84.7
MS.gene069870.t1	AT1G54095	37.410	139	75	4	62	190	14	150	5.74e-20	82.4
MS.gene069870.t1	AT5G20670	33.846	130	80	3	64	187	23	152	3.32e-18	77.8

Find 35 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGTTAGATTTAATAAATT+TGG	0.209428	2.1:+3872463	MS.gene069870:CDS
AGCTTCTATATAAGATTAAT+TGG	0.236977	2.1:-3872674	None:intergenic
GCATCTTCAACTTCAGTTAT+CGG	0.334901	2.1:-3872265	None:intergenic
AGTTGAGATGAATTAGGTTT+TGG	0.358947	2.1:-3872157	None:intergenic
GTGACAAATACTTAGGGAAA+TGG	0.368899	2.1:+3872347	MS.gene069870:CDS
GCTTCAGCTTGAAACAAAAC+AGG	0.390939	2.1:-3872496	None:intergenic
CGTGTACGTGACAAATACTT+AGG	0.417201	2.1:+3872340	MS.gene069870:CDS
TTGTTGAGTTGAGATGAATT+AGG	0.428382	2.1:-3872163	None:intergenic
TTTCACCACCTTTGTCTCTA+GGG	0.429554	2.1:-3872576	None:intergenic
TGACAAATACTTAGGGAAAT+GGG	0.461618	2.1:+3872348	MS.gene069870:CDS
TGAGGCAGTGAAAGAAGAGT+TGG	0.465910	2.1:+3872387	MS.gene069870:CDS
TTTGGAGCATACAATGTTGT+TGG	0.466523	2.1:-3872131	None:intergenic
TACTTAGGGAAATGGGTTTG+TGG	0.476568	2.1:+3872355	MS.gene069870:CDS
GAGTAAAATGGAAGGGAGAA+GGG	0.485393	2.1:+3872540	MS.gene069870:CDS
TTGATATCTCTTGTAATAGC+AGG	0.500356	2.1:-3872637	None:intergenic
AGAGTAAAATGGAAGGGAGA+AGG	0.512738	2.1:+3872539	MS.gene069870:CDS
GTGTACGTGACAAATACTTA+GGG	0.526950	2.1:+3872341	MS.gene069870:CDS
TCAACTATTATGGCACCTCA+TGG	0.537712	2.1:+3872079	None:intergenic
GTGGAAGCTGTGTTAAATCA+AGG	0.538899	2.1:-3872243	None:intergenic
GGTGGTGAAAAGAAAGGTGG+TGG	0.545436	2.1:+3872589	MS.gene069870:CDS
TTAGATTTAATAAATTTGGA+AGG	0.546403	2.1:+3872467	MS.gene069870:CDS
CATTACCTAGCTCAATCTCT+AGG	0.548665	2.1:+3872107	MS.gene069870:CDS
GACAAAGGTGGTGAAAAGAA+AGG	0.558067	2.1:+3872583	MS.gene069870:CDS
TTGGTCCTAGAGATTGAGCT+AGG	0.570237	2.1:-3872112	None:intergenic
GGTTTGTGGTTTGTGTTCTG+AGG	0.597680	2.1:+3872369	MS.gene069870:CDS
TGGTGGGGAAAATATTGAAG+AGG	0.600750	2.1:+3872417	MS.gene069870:CDS
TCTTCAACTTCAGTTATCGG+TGG	0.626778	2.1:-3872262	None:intergenic
TCATTCAACCCTAGAGACAA+AGG	0.638410	2.1:+3872568	MS.gene069870:CDS
CTTCAGCTTGAAACAAAACA+GGG	0.640341	2.1:-3872495	None:intergenic
GTGGTGAAAAGAAAGGTGGT+GGG	0.641104	2.1:+3872590	MS.gene069870:CDS
AAAGGTGGTGAAAAGAAAGG+TGG	0.652842	2.1:+3872586	MS.gene069870:CDS
GTGTGAATGCTGTGGCATGT+CGG	0.682909	2.1:+3872291	MS.gene069870:CDS
GATGCAAAGTGTGAATGCTG+TGG	0.733859	2.1:+3872283	MS.gene069870:CDS
TTCAACCCTAGAGACAAAGG+TGG	0.741656	2.1:+3872571	MS.gene069870:CDS
CTAGGTAATGAATCTCCATG+AGG	0.751297	2.1:-3872094	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGTGTTAGATTTAATAAATT+TGG	+	chr2.1:3872463-3872482	MS.gene069870:CDS	15.0%
!!	TTAGATTTAATAAATTTGGA+AGG	+	chr2.1:3872467-3872486	MS.gene069870:CDS	15.0%
!	TGAAAAAGAGTAAAATGGAA+GGG	+	chr2.1:3872533-3872552	MS.gene069870:CDS	25.0%
	AAAGAAGAGTTGGAGAAAAA+TGG	+	chr2.1:3872397-3872416	MS.gene069870:CDS	30.0%
	CTGAAAAAGAGTAAAATGGA+AGG	+	chr2.1:3872532-3872551	MS.gene069870:CDS	30.0%
	GATGCTGAAAAAGAGTAAAA+TGG	+	chr2.1:3872528-3872547	MS.gene069870:CDS	30.0%
	TGACAAATACTTAGGGAAAT+GGG	+	chr2.1:3872348-3872367	MS.gene069870:CDS	30.0%
	TTGATATCTCTTGTAATAGC+AGG	-	chr2.1:3872640-3872659	None:intergenic	30.0%
!	AGTTGAGATGAATTAGGTTT+TGG	-	chr2.1:3872160-3872179	None:intergenic	30.0%
!!	TTGTTGAGTTGAGATGAATT+AGG	-	chr2.1:3872166-3872185	None:intergenic	30.0%
	AAGAGTTGGAGAAAAATGGT+GGG	+	chr2.1:3872401-3872420	MS.gene069870:CDS	35.0%
	CTTCAGCTTGAAACAAAACA+GGG	-	chr2.1:3872498-3872517	None:intergenic	35.0%
	GCATCTTCAACTTCAGTTAT+CGG	-	chr2.1:3872268-3872287	None:intergenic	35.0%
	GTGACAAATACTTAGGGAAA+TGG	+	chr2.1:3872347-3872366	MS.gene069870:CDS	35.0%
	GTGTACGTGACAAATACTTA+GGG	+	chr2.1:3872341-3872360	MS.gene069870:CDS	35.0%
!	CGTACACGTTTTATATACTC+AGG	-	chr2.1:3872328-3872347	None:intergenic	35.0%
!	TTTGGAGCATACAATGTTGT+TGG	-	chr2.1:3872134-3872153	None:intergenic	35.0%
!!	TGAATTAGGTTTTGGTGGTT+TGG	-	chr2.1:3872152-3872171	None:intergenic	35.0%
!!	TGAGATGAATTAGGTTTTGG+TGG	-	chr2.1:3872157-3872176	None:intergenic	35.0%
	AAAGGTGGTGAAAAGAAAGG+TGG	+	chr2.1:3872586-3872605	MS.gene069870:CDS	40.0%
	AGAGTAAAATGGAAGGGAGA+AGG	+	chr2.1:3872539-3872558	MS.gene069870:CDS	40.0%
	AGAGTTGGAGAAAAATGGTG+GGG	+	chr2.1:3872402-3872421	MS.gene069870:CDS	40.0%
	CATTACCTAGCTCAATCTCT+AGG	+	chr2.1:3872107-3872126	MS.gene069870:CDS	40.0%
	CGTGTACGTGACAAATACTT+AGG	+	chr2.1:3872340-3872359	MS.gene069870:CDS	40.0%
	CTAGGTAATGAATCTCCATG+AGG	-	chr2.1:3872097-3872116	None:intergenic	40.0%
	GAAGAGTTGGAGAAAAATGG+TGG	+	chr2.1:3872400-3872419	MS.gene069870:CDS	40.0%
	GACAAAGGTGGTGAAAAGAA+AGG	+	chr2.1:3872583-3872602	MS.gene069870:CDS	40.0%
	GAGTAAAATGGAAGGGAGAA+GGG	+	chr2.1:3872540-3872559	MS.gene069870:CDS	40.0%
	GCTTCAGCTTGAAACAAAAC+AGG	-	chr2.1:3872499-3872518	None:intergenic	40.0%
	GTGGAAGCTGTGTTAAATCA+AGG	-	chr2.1:3872246-3872265	None:intergenic	40.0%
	TCATTCAACCCTAGAGACAA+AGG	+	chr2.1:3872568-3872587	MS.gene069870:CDS	40.0%
	TCTTCAACTTCAGTTATCGG+TGG	-	chr2.1:3872265-3872284	None:intergenic	40.0%
	TGGTGGGGAAAATATTGAAG+AGG	+	chr2.1:3872417-3872436	MS.gene069870:CDS	40.0%
	TTTCACCACCTTTGTCTCTA+GGG	-	chr2.1:3872579-3872598	None:intergenic	40.0%
!	TACTTAGGGAAATGGGTTTG+TGG	+	chr2.1:3872355-3872374	MS.gene069870:CDS	40.0%
!	TTTTCACCACCTTTGTCTCT+AGG	-	chr2.1:3872580-3872599	None:intergenic	40.0%
	GATGCAAAGTGTGAATGCTG+TGG	+	chr2.1:3872283-3872302	MS.gene069870:CDS	45.0%
	GTGGTGAAAAGAAAGGTGGT+GGG	+	chr2.1:3872590-3872609	MS.gene069870:CDS	45.0%
	TGAGGCAGTGAAAGAAGAGT+TGG	+	chr2.1:3872387-3872406	MS.gene069870:CDS	45.0%
	TTCAACCCTAGAGACAAAGG+TGG	+	chr2.1:3872571-3872590	MS.gene069870:CDS	45.0%
	TTGGTCCTAGAGATTGAGCT+AGG	-	chr2.1:3872115-3872134	None:intergenic	45.0%
!	GGTTTGTGGTTTGTGTTCTG+AGG	+	chr2.1:3872369-3872388	MS.gene069870:CDS	45.0%
	GGTGGTGAAAAGAAAGGTGG+TGG	+	chr2.1:3872589-3872608	MS.gene069870:CDS	50.0%
	GTGTGAATGCTGTGGCATGT+CGG	+	chr2.1:3872291-3872310	MS.gene069870:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	3872088	3872681	3872088	ID=MS.gene069870
chr2.1	mRNA	3872088	3872681	3872088	ID=MS.gene069870.t1;Parent=MS.gene069870
chr2.1	exon	3872088	3872681	3872088	ID=MS.gene069870.t1.exon1;Parent=MS.gene069870.t1
chr2.1	CDS	3872088	3872681	3872088	ID=cds.MS.gene069870.t1;Parent=MS.gene069870.t1

Gene Sequence

>MS.gene069870

ATGGCACCTCATGGAGATTCATTACCTAGCTCAATCTCTAGGACCAACAACATTGTATGCTCCAAACCACCAAAACCTAATTCATCTCAACTCAACAACAACAATAATATTCAAAGAACAATCTCAGACATATCATTTGAACTATCAAAACAAGACCTTGATTTAACACAGCTTCCACCGATAACTGAAGTTGAAGATGCAAAGTGTGAATGCTGTGGCATGTCGGAAGAGTTTACACCTGAGTATATAAAACGTGTACGTGACAAATACTTAGGGAAATGGGTTTGTGGTTTGTGTTCTGAGGCAGTGAAAGAAGAGTTGGAGAAAAATGGTGGGGAAAATATTGAAGAGGCTTTGAGTGCACACATGAGTGCATGTGTTAGATTTAATAAATTTGGAAGGAGTTTCCCTGTTTTGTTTCAAGCTGAAGCTATGAAAGAGATGCTGAAAAAGAGTAAAATGGAAGGGAGAAGGGCTAAGTCATTCAACCCTAGAGACAAAGGTGGTGAAAAGAAAGGTGGTGGGCTTGCTAGAAGTTCAAGTTGCATTCCTGCTATTACAAGAGATATCAATGATTTCACAATCACCAATTAA

Protein sequence

>MS.gene069870.t1

MAPHGDSLPSSISRTNNIVCSKPPKPNSSQLNNNNNIQRTISDISFELSKQDLDLTQLPPITEVEDAKCECCGMSEEFTPEYIKRVRDKYLGKWVCGLCSEAVKEELEKNGGENIEEALSAHMSACVRFNKFGRSFPVLFQAEAMKEMLKKSKMEGRRAKSFNPRDKGGEKKGGGLARSSSCIPAITRDINDFTITN