AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072591


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072591.t1 MTR_7g111590 99.034 207 2 0 1 207 1 207 2.65e-151 418
MS.gene072591.t1 MTR_5g026750 98.551 207 3 0 1 207 1 207 1.30e-150 416
MS.gene072591.t1 MTR_1g022165 97.585 207 5 0 1 207 1 207 3.13e-149 413
MS.gene072591.t1 MTR_7g111590 98.068 207 2 1 1 207 1 205 7.49e-148 409
MS.gene072591.t1 MTR_8g071810 64.198 81 29 0 104 184 67 147 3.47e-27 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072591.t1 AT3G49010 87.435 191 24 0 2 192 1 191 2.38e-126 355
MS.gene072591.t1 AT3G49010 87.435 191 24 0 2 192 1 191 2.38e-126 355
MS.gene072591.t1 AT3G49010 87.435 191 24 0 2 192 1 191 2.38e-126 355
MS.gene072591.t1 AT3G49010 87.435 191 24 0 2 192 1 191 2.38e-126 355
MS.gene072591.t1 AT3G49010 87.435 191 24 0 2 192 1 191 2.38e-126 355
MS.gene072591.t1 AT3G49010 86.387 191 24 1 2 192 1 189 4.01e-123 347
MS.gene072591.t1 AT5G23900 85.484 186 27 0 2 187 1 186 1.65e-119 338
MS.gene072591.t1 AT3G48960 77.083 192 44 0 2 193 1 192 4.54e-109 311
MS.gene072591.t1 AT3G49010 61.257 191 21 1 2 192 1 138 3.76e-78 231

Find 0 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTATAATAATATCATC+AGG + 50590:1422-1441 None:intergenic 10.0%
!! AAAAACAAACTCAATTAAAT+TGG + 50590:1607-1626 None:intergenic 15.0%
!! TTTAAGAATATGTAACAAAA+TGG - 50590:1334-1353 MS.gene072591:intron 15.0%
!!! AAATTCTAGATATTTACTTT+TGG - 50590:1169-1188 MS.gene072591:CDS 15.0%
!!! TTAATTGAGTTTGTTTTTTA+AGG - 50590:1609-1628 MS.gene072591:intron 15.0%
!!! TTTCTTCTATTTTATAATGT+AGG - 50590:2070-2089 MS.gene072591:intron 15.0%
!! AAACTAAAAACTCTTAAAAC+CGG + 50590:1704-1723 None:intergenic 20.0%
!! TAAGAAATAAACCATTGAAA+AGG + 50590:2050-2069 None:intergenic 20.0%
!! TAAGATTACAAATGAAATGA+AGG - 50590:2481-2500 MS.gene072591:intron 20.0%
!!! AAGAAATCATGGATTATATA+AGG + 50590:1820-1839 None:intergenic 20.0%
!!! TATGACCTTTTTCTTAATTT+TGG - 50590:2282-2301 MS.gene072591:CDS 20.0%
!!! TTTTAGCATTATCAAAAAAG+TGG - 50590:1133-1152 MS.gene072591:CDS 20.0%
!!! TTTTTACTTTACCTTTTCAA+TGG - 50590:2036-2055 MS.gene072591:intron 20.0%
! AAATCTACTTTAGTTGTTAC+AGG - 50590:1743-1762 MS.gene072591:intron 25.0%
! AAATTGCAAGTCACTAAAAA+CGG - 50590:1232-1251 MS.gene072591:intron 25.0%
! AACTAAAAACTCTTAAAACC+GGG + 50590:1703-1722 None:intergenic 25.0%
! AGAAATACAACATGAAACTA+AGG - 50590:1025-1044 MS.gene072591:intron 25.0%
! ATAGGTAAAAATACTCCATA+AGG + 50590:1572-1591 None:intergenic 25.0%
! GAAATACAACATGAAACTAA+GGG - 50590:1026-1045 MS.gene072591:intron 25.0%
! GCATTATTCTTAATTTCTTC+TGG - 50590:2344-2363 MS.gene072591:CDS 25.0%
! GTGTATAAGTAAAGAAATCA+TGG + 50590:1831-1850 None:intergenic 25.0%
! TTCAAATTTATCAGCAATGA+CGG + 50590:2310-2329 None:intergenic 25.0%
!! AAATCATGGATTATATAAGG+AGG + 50590:1817-1836 None:intergenic 25.0%
!! AATATGATTAGTGTTTTCAG+TGG + 50590:1853-1872 None:intergenic 25.0%
!! ACAACTAAAGTAGATTTTGA+TGG + 50590:1740-1759 None:intergenic 25.0%
!! TTTGATTTACTTGACTTGAT+GGG - 50590:909-928 MS.gene072591:CDS 25.0%
!!! AAATGAAATGAAGGCATTTA+AGG - 50590:2490-2509 MS.gene072591:intron 25.0%
!!! AGTATTTTTACCTATAGGAT+TGG - 50590:1577-1596 MS.gene072591:intron 25.0%
!!! TATGGAGTATTTTTACCTAT+AGG - 50590:1572-1591 MS.gene072591:intron 25.0%
CGGTACCAAAATTAAGAAAA+AGG + 50590:2290-2309 None:intergenic 30.0%
GACAGAATTGAATATGAAAC+AGG + 50590:1921-1940 None:intergenic 30.0%
TAAAAATACTCCATAAGGAC+AGG + 50590:1567-1586 None:intergenic 30.0%
TCACAACTGAAGAGAATATT+CGG + 50590:1898-1917 None:intergenic 30.0%
TTCATACTTACCAATTGTGA+GGG - 50590:2436-2455 MS.gene072591:intron 30.0%
! CTTTGATTTACTTGACTTGA+TGG - 50590:908-927 MS.gene072591:CDS 30.0%
! TATTTTATAATGTAGGCTGC+TGG - 50590:2077-2096 MS.gene072591:intron 30.0%
!!! AGTTTGTTTTTTAAGGCCTA+TGG - 50590:1616-1635 MS.gene072591:intron 30.0%
AACCCTGACAATCAAGTTAT+AGG - 50590:1666-1685 MS.gene072591:intron 35.0%
AGACCTATAACTTGATTGTC+AGG + 50590:1672-1691 None:intergenic 35.0%
ATCTTAACAAGCTCTACAGA+TGG + 50590:2467-2486 None:intergenic 35.0%
CAAGCTCTATGTGAAATTAC+AGG - 50590:1389-1408 MS.gene072591:intron 35.0%
CAGCCTGGAAATAAAAAACA+GGG + 50590:2370-2389 None:intergenic 35.0%
GACCTATAACTTGATTGTCA+GGG + 50590:1671-1690 None:intergenic 35.0%
GTTCATACTTACCAATTGTG+AGG - 50590:2435-2454 MS.gene072591:intron 35.0%
TACTAGATGCACTCAAACTA+AGG + 50590:1979-1998 None:intergenic 35.0%
TCGACAGAAATACCAATAGT+TGG + 50590:2122-2141 None:intergenic 35.0%
! AAAAAGCTTGCTCCAACTAT+TGG - 50590:2107-2126 MS.gene072591:intron 35.0%
! AATAGTTGGAGCAAGCTTTT+TGG + 50590:2108-2127 None:intergenic 35.0%
! ATAGTTGGAGCAAGCTTTTT+GGG + 50590:2107-2126 None:intergenic 35.0%
AAAAACTCTTAAAACCGGGC+AGG + 50590:1699-1718 None:intergenic 40.0%
AAAACTCTTAAAACCGGGCA+GGG + 50590:1698-1717 None:intergenic 40.0%
AATACTCCATAAGGACAGGT+TGG + 50590:1563-1582 None:intergenic 40.0%
ACTTGACAACAGGTTTGTAC+TGG + 50590:1537-1556 None:intergenic 40.0%
AGTACAAACCTGTTGTCAAG+TGG - 50590:1536-1555 MS.gene072591:intron 40.0%
CCAGCCTGGAAATAAAAAAC+AGG + 50590:2371-2390 None:intergenic 40.0%
CGAACAAACAAACGCCATTT+GGG - 50590:2536-2555 MS.gene072591:CDS 40.0%
GCAAACGCAATTCCATGTAT+TGG + 50590:1497-1516 None:intergenic 40.0%
GGCAAAACTATGTCAAGACA+TGG - 50590:774-793 MS.gene072591:CDS 40.0%
GGTCAAGGTAATGCAAATTG+CGG - 50590:2244-2263 MS.gene072591:intron 40.0%
GTTTACCTTCAACTCCTCAA+GGG + 50590:1067-1086 None:intergenic 40.0%
TACAACATGAAACTAAGGGC+TGG - 50590:1030-1049 MS.gene072591:intron 40.0%
TGAGTCTGTCCATGAACAAT+AGG + 50590:1006-1025 None:intergenic 40.0%
TGATTGTCAGGGTTCCTAAA+CGG + 50590:1660-1679 None:intergenic 40.0%
TGTTTACCTTCAACTCCTCA+AGG + 50590:1068-1087 None:intergenic 40.0%
TTGCAATCCACACCAATACA+TGG - 50590:1482-1501 MS.gene072591:intron 40.0%
! AATTGGCTAGCCAATCCTAT+AGG + 50590:1590-1609 None:intergenic 40.0%
! ATGAAACTAAGGGCTGGTAA+AGG - 50590:1036-1055 MS.gene072591:intron 40.0%
! ATTTTCAGCTCGTCAGAAGA+AGG - 50590:939-958 MS.gene072591:intron 40.0%
! TGTATTGGTGTGGATTGCAA+AGG + 50590:1482-1501 None:intergenic 40.0%
!! CATAGTTTTGCCAATGCTTG+CGG + 50590:766-785 None:intergenic 40.0%
!! GTAGATTTTGATGGTCATGC+TGG + 50590:1731-1750 None:intergenic 40.0%
!!! CCTGTTTTTTATTTCCAGGC+TGG - 50590:2368-2387 MS.gene072591:intron 40.0%
!!! TGGCCCTGTTTTTTATTTCC+AGG - 50590:2364-2383 MS.gene072591:CDS 40.0%
!!! TGTTTTTTAAGGCCTATGGC+TGG - 50590:1620-1639 MS.gene072591:intron 40.0%
AGGCCTTGGGAAAATCTTCA+CGG + 50590:965-984 None:intergenic 45.0%
ATGAACACTTCCGCAAGCAT+TGG - 50590:753-772 MS.gene072591:CDS 45.0%
CACCAATCTCCAATGTACGT+AGG - 50590:1787-1806 MS.gene072591:intron 45.0%
CACCTACGTACATTGGAGAT+TGG + 50590:1792-1811 None:intergenic 45.0%
CATGGTTTAACCAACCTGCA+AGG - 50590:792-811 MS.gene072591:CDS 45.0%
CCTAAACGGCATACGAGTTA+AGG + 50590:1646-1665 None:intergenic 45.0%
GAAGACATACAAGGCCAAGT+TGG - 50590:2196-2215 MS.gene072591:intron 45.0%
GCAAATGCTACACAGGTTCA+AGG - 50590:2413-2432 MS.gene072591:intron 45.0%
GCGAACAAACAAACGCCATT+TGG - 50590:2535-2554 MS.gene072591:CDS 45.0%
GGAACTAGCAAATGCTACAC+AGG - 50590:2406-2425 MS.gene072591:intron 45.0%
! ATTTGCTAGTTCCTCAGGAG+TGG + 50590:2399-2418 None:intergenic 45.0%
! CCTTAACTCGTATGCCGTTT+AGG - 50590:1643-1662 MS.gene072591:intron 45.0%
! CGCAATTCCATGTATTGGTG+TGG + 50590:1492-1511 None:intergenic 45.0%
! GTAGCATTTGCTAGTTCCTC+AGG + 50590:2404-2423 None:intergenic 45.0%
!! GCTTGCGGAAGTGTTCATTA+GGG + 50590:751-770 None:intergenic 45.0%
!! TGCTTGCGGAAGTGTTCATT+AGG + 50590:752-771 None:intergenic 45.0%
AGGAAGACGAGAAGACGCTT+AGG - 50590:812-831 MS.gene072591:CDS 50.0%
AGTGGACCAACCTGTCCTTA+TGG - 50590:1554-1573 MS.gene072591:intron 50.0%
ATTTGGGTGCCAGACTGAAG+AGG - 50590:2552-2571 MS.gene072591:CDS 50.0%
CCACTCAGGCCTATTGTTCA+TGG - 50590:994-1013 MS.gene072591:intron 50.0%
CCATGAACAATAGGCCTGAG+TGG + 50590:997-1016 None:intergenic 50.0%
CTTCTCCCTTGAGGAGTTGA+AGG - 50590:1059-1078 MS.gene072591:intron 50.0%
CTTGCAGACCTTCCAAGGAT+CGG + 50590:2160-2179 None:intergenic 50.0%
CTTGTCTTCCTTCTCTGCCT+CGG + 50590:2582-2601 None:intergenic 50.0%
GATGGCTTCTCCCTCACAAT+TGG + 50590:2449-2468 None:intergenic 50.0%
GCAGAGGCTGAAGACATACA+AGG - 50590:2187-2206 MS.gene072591:intron 50.0%
GGAGGTTCACCTACGTACAT+TGG + 50590:1799-1818 None:intergenic 50.0%
TACGAGTTAAGGCCAGCCAT+AGG + 50590:1635-1654 None:intergenic 50.0%
TCTTCAGTCTGGCACCCAAA+TGG + 50590:2553-2572 None:intergenic 50.0%
TCTTGGGAAGACGACCAACT+TGG + 50590:2213-2232 None:intergenic 50.0%
TGGTAAAGGCTTCTCCCTTG+AGG - 50590:1050-1069 MS.gene072591:intron 50.0%
TTATAGGTCTATGCCCTGCC+CGG - 50590:1682-1701 MS.gene072591:intron 50.0%
TTCTCGTCTTCCTTGCAGGT+TGG + 50590:805-824 None:intergenic 50.0%
TTTGGGTGCCAGACTGAAGA+GGG - 50590:2553-2572 MS.gene072591:CDS 50.0%
! AGGCCGTGAAGATTTTCCCA+AGG - 50590:959-978 MS.gene072591:intron 50.0%
! AGGTTGGTCCACTTGACAAC+AGG + 50590:1547-1566 None:intergenic 50.0%
! ATTTTCCCAAGGCCTACAGC+TGG - 50590:970-989 MS.gene072591:intron 50.0%
! CTTCCAAGGATCGGTTCTTG+CGG + 50590:2151-2170 None:intergenic 50.0%
AAGACGTGCACGCAAGGTCA+AGG - 50590:2229-2248 MS.gene072591:intron 55.0%
CGCAAGAACCGATCCTTGGA+AGG - 50590:2149-2168 MS.gene072591:intron 55.0%
CGTCTTCTCGTCTTCCTTGC+AGG + 50590:809-828 None:intergenic 55.0%
TGACCTTGCGTGCACGTCTT+GGG + 50590:2229-2248 None:intergenic 55.0%
TGTCTTCAGCCTCTGCACGT+TGG + 50590:2183-2202 None:intergenic 55.0%
TTGACCTTGCGTGCACGTCT+TGG + 50590:2230-2249 None:intergenic 55.0%
!! GTTGGCTTGCAGACCTTCCA+AGG + 50590:2165-2184 None:intergenic 55.0%
AGTGGTCCAGCTGTAGGCCT+TGG + 50590:979-998 None:intergenic 60.0%
CAGGAGTGGAATCACCAGCC+TGG + 50590:2385-2404 None:intergenic 60.0%
CTTCCCAAGACGTGCACGCA+AGG - 50590:2223-2242 MS.gene072591:intron 60.0%
GGCTGGTGATTCCACTCCTG+AGG - 50590:2385-2404 MS.gene072591:intron 60.0%
GTCTGCAAGCCAACGTGCAG+AGG - 50590:2171-2190 MS.gene072591:intron 60.0%
GTGGTCCAGCTGTAGGCCTT+GGG + 50590:978-997 None:intergenic 60.0%
TCGCCGCAAGAACCGATCCT+TGG - 50590:2145-2164 MS.gene072591:intron 60.0%
CAGACTGAAGAGGGCTGCCG+AGG - 50590:2562-2581 MS.gene072591:CDS 65.0%
CTCGGCAGCCCTCTTCAGTC+TGG + 50590:2564-2583 None:intergenic 65.0%
GGCCTACAGCTGGACCACTC+AGG - 50590:980-999 MS.gene072591:intron 65.0%
GGCCTGAGTGGTCCAGCTGT+AGG + 50590:985-1004 None:intergenic 65.0%
GAGGGCTGCCGAGGCAGAGA+AGG - 50590:2571-2590 MS.gene072591:CDS 70.0%


Chromosome Type Strat End Strand Name
50590 gene 725 2607 725 ID=MS.gene072591
50590 mRNA 725 2607 725 ID=MS.gene072591.t1;Parent=MS.gene072591
50590 exon 2499 2607 2499 ID=MS.gene072591.t1.exon1;Parent=MS.gene072591.t1
50590 CDS 2499 2607 2499 ID=cds.MS.gene072591.t1;Parent=MS.gene072591.t1
50590 exon 2252 2385 2252 ID=MS.gene072591.t1.exon2;Parent=MS.gene072591.t1
50590 CDS 2252 2385 2252 ID=cds.MS.gene072591.t1;Parent=MS.gene072591.t1
50590 exon 1082 1240 1082 ID=MS.gene072591.t1.exon3;Parent=MS.gene072591.t1
50590 CDS 1082 1240 1082 ID=cds.MS.gene072591.t1;Parent=MS.gene072591.t1
50590 exon 725 946 725 ID=MS.gene072591.t1.exon4;Parent=MS.gene072591.t1
50590 CDS 725 946 725 ID=cds.MS.gene072591.t1;Parent=MS.gene072591.t1
Gene Sequence

>MS.gene072591

ATGGTGAAGCATAACAATGTTATCCCTAATGAACACTTCCGCAAGCATTGGCAAAACTATGTCAAGACATGGTTTAACCAACCTGCAAGGAAGACGAGAAGACGCTTAGGTAATTTTCTCTATAATTCTATTATTTTGATTAATTAAGTTTTATGTATTGAAAATTGAAAATAAAAATGTTAACTTTGATTTACTTGACTTGATGGGTTGTTGCATTTTCAGCTCGTCAGAAGAAGGCCGTGAAGATTTTCCCAAGGCCTACAGCTGGACCACTCAGGCCTATTGTTCATGGACAGACTCAGAAATACAACATGAAACTAAGGGCTGGTAAAGGCTTCTCCCTTGAGGAGTTGAAGGTAAACATCTTTAACTTCTGTGATTTTTCTTTTCGATGTAATTTATTGAACGTTTTAGCATTATCAAAAAAGTGGTTTTGATGCTCTAAAATTCTAGATATTTACTTTTGGAGATTTAAAGAAAATATAGAAGCTGTTGTGTTTGTTTACGAAATTGCAAGTCACTAAAAACGGTTATTGAAAATGATTTTCAAGAGAAGCTACGCAAAACAGCTTCTCATTTGAAGCTTTGATTATTAACATGTTTTCATTTTTTAAGAATATGTAACAAAATGGATGAAAACTTTTGAAAGTGAATTTTTTACAAACAAGCTCTATGTGAAATTACAGGAACTCTACCTGATGATATTATTATAATTTAAAAGATATAATCTAATTTTCTGTTTGATGTACTTCTGCCTTTGCAATCCACACCAATACATGGAATTGCGTTTGCAAAATTCTCTCTTAATTCCAGTACAAACCTGTTGTCAAGTGGACCAACCTGTCCTTATGGAGTATTTTTACCTATAGGATTGGCTAGCCAATTTAATTGAGTTTGTTTTTTAAGGCCTATGGCTGGCCTTAACTCGTATGCCGTTTAGGAACCCTGACAATCAAGTTATAGGTCTATGCCCTGCCCGGTTTTAAGAGTTTTTAGTTTTTATCCAGCATGACCATCAAAATCTACTTTAGTTGTTACAGGACTATTATACTTATGATATTACACCAATCTCCAATGTACGTAGGTGAACCTCCTTATATAATCCATGATTTCTTTACTTATACACCACTGAAAACACTAATCATATTATAGACAGTTTGAGTGAGTTCTCCGAATATTCTCTTCAGTTGTGACCTGTTTCATATTCAATTCTGTCTAATTTTAGCTTCAAGATGTTAAAAGAGTAAAAGACCTTAGTTTGAGTGCATCTAGTATAGAGTATAACTATGCAATGTACTTTGATATTCTGCTTTTTTACTTTACCTTTTCAATGGTTTATTTCTTATTTCTTCTATTTTATAATGTAGGCTGCTGGTATTCCCAAAAAGCTTGCTCCAACTATTGGTATTTCTGTCGATCATCGCCGCAAGAACCGATCCTTGGAAGGTCTGCAAGCCAACGTGCAGAGGCTGAAGACATACAAGGCCAAGTTGGTCGTCTTCCCAAGACGTGCACGCAAGGTCAAGGTAATGCAAATTGCGGCTATATGCTTTAATATATGACCTTTTTCTTAATTTTGGTACCGTCATTGCTGATAAATTTGAAAAAGAAAAAGTTGTGCATTATTCTTAATTTCTTCTGGCCCTGTTTTTTATTTCCAGGCTGGTGATTCCACTCCTGAGGAACTAGCAAATGCTACACAGGTTCAAGGTTCATACTTACCAATTGTGAGGGAGAAGCCATCTGTAGAGCTTGTTAAGATTACAAATGAAATGAAGGCATTTAAGGCTTATTACAAGCTTCGTCTTGAGCGAACAAACAAACGCCATTTGGGTGCCAGACTGAAGAGGGCTGCCGAGGCAGAGAAGGAAGACAAGAAGTGA

Protein sequence

>MS.gene072591.t1

MVKHNNVIPNEHFRKHWQNYVKTWFNQPARKTRRRLARQKKAVKIFPRPTAGPLRPIVHGQTQKYNMKLRAGKGFSLEELKAAGIPKKLAPTIGISVDHRRKNRSLEGLQANVQRLKTYKAKLVVFPRRARKVKAGDSTPEELANATQVQGSYLPIVREKPSVELVKITNEMKAFKAYYKLRLERTNKRHLGARLKRAAEAEKEDKK