AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08514


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08514.t1 MTR_8g024210 99.459 185 1 0 1 185 181 365 2.03e-132 375
MS.gene08514.t1 MTR_0194s0050 54.000 200 73 5 1 182 184 382 2.65e-60 192
MS.gene08514.t1 MTR_7g113830 51.282 195 85 2 1 185 116 310 3.10e-60 190
MS.gene08514.t1 MTR_1g082480 50.769 195 82 3 1 181 136 330 3.89e-60 191
MS.gene08514.t1 MTR_3g099620 49.239 197 84 3 1 181 116 312 4.28e-60 190
MS.gene08514.t1 MTR_8g066770 49.741 193 82 3 1 181 114 303 2.46e-58 185
MS.gene08514.t1 MTR_5g064950 46.392 194 82 4 1 177 100 288 1.81e-53 172
MS.gene08514.t1 MTR_1g082480 50.000 172 72 3 1 158 136 307 5.91e-49 162
MS.gene08514.t1 MTR_5g064950 46.857 175 71 4 1 158 100 269 4.40e-47 156
MS.gene08514.t1 MTR_2g022280 44.920 187 94 3 1 179 1 186 3.09e-44 145
MS.gene08514.t1 MTR_3g090850 39.565 230 85 6 1 178 101 328 5.03e-40 138
MS.gene08514.t1 MTR_1g028470 35.749 207 103 3 1 178 87 292 1.16e-35 126
MS.gene08514.t1 MTR_1g072320 27.957 186 124 4 3 180 471 654 1.19e-14 71.6
MS.gene08514.t1 MTR_8g027495 27.160 162 95 5 1 157 457 600 2.14e-13 67.8
MS.gene08514.t1 MTR_5g014520 29.530 149 100 2 3 151 158 301 5.48e-13 66.2
MS.gene08514.t1 MTR_4g094762 27.907 172 101 4 3 160 293 455 8.83e-12 63.2
MS.gene08514.t1 MTR_7g083900 27.273 187 93 7 3 165 287 454 1.33e-11 62.8
MS.gene08514.t1 MTR_1g112680 27.957 186 101 6 3 160 318 498 5.11e-11 60.8
MS.gene08514.t1 MTR_2g104550 26.816 179 125 4 1 178 137 310 6.84e-11 60.1
MS.gene08514.t1 MTR_7g083900 27.273 187 92 7 3 165 287 453 8.71e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08514.t1 AT2G46810 56.593 182 70 4 1 178 121 297 3.14e-64 200
MS.gene08514.t1 AT2G46810 56.593 182 70 4 1 178 181 357 2.01e-63 199
MS.gene08514.t1 AT2G46810 56.593 182 70 4 1 178 193 369 2.95e-63 199
MS.gene08514.t1 AT4G01460 52.062 194 77 3 1 182 115 304 9.54e-62 194
MS.gene08514.t1 AT4G01460 52.062 194 77 3 1 182 115 304 9.54e-62 194
MS.gene08514.t1 AT2G46810 49.048 210 70 5 1 178 121 325 3.87e-58 185
MS.gene08514.t1 AT3G24140 50.990 202 78 3 1 182 197 397 6.50e-57 184
MS.gene08514.t1 AT3G24140 51.244 201 77 3 1 181 197 396 7.56e-57 184
MS.gene08514.t1 AT1G22490 49.479 192 80 3 1 181 115 300 1.91e-55 177
MS.gene08514.t1 AT1G72210 48.947 190 87 3 1 181 125 313 2.93e-54 175
MS.gene08514.t1 AT3G61950 52.486 181 78 3 1 178 127 302 4.14e-54 174
MS.gene08514.t1 AT3G61950 52.486 181 78 3 1 178 178 353 2.41e-53 174
MS.gene08514.t1 AT1G22490 50.296 169 67 3 1 158 115 277 4.63e-48 159
MS.gene08514.t1 AT1G72210 48.503 167 76 3 1 158 125 290 7.66e-47 155
MS.gene08514.t1 AT3G61950 52.795 161 68 3 1 158 127 282 1.49e-45 152
MS.gene08514.t1 AT3G61950 52.795 161 68 3 1 158 178 333 7.98e-45 151
MS.gene08514.t1 AT5G46690 44.811 212 86 5 1 181 88 299 1.50e-43 147
MS.gene08514.t1 AT3G06120 43.017 179 87 2 1 164 1 179 1.76e-41 139
MS.gene08514.t1 AT5G65320 40.107 187 102 4 1 177 102 288 3.21e-36 128
MS.gene08514.t1 AT5G46690 43.915 189 75 5 1 158 88 276 2.96e-34 122
MS.gene08514.t1 AT5G65320 39.535 172 85 4 1 158 102 268 9.99e-30 110
MS.gene08514.t1 AT5G53210 66.667 63 21 0 1 63 102 164 6.87e-25 99.4
MS.gene08514.t1 AT5G53210 66.667 63 21 0 1 63 102 164 7.72e-25 99.4
MS.gene08514.t1 AT5G53210 40.000 90 53 1 89 178 275 363 1.71e-12 65.1
MS.gene08514.t1 AT3G26744 30.857 175 99 6 3 165 308 472 2.77e-13 67.4
MS.gene08514.t1 AT3G26744 30.857 175 99 6 3 165 308 472 2.77e-13 67.4
MS.gene08514.t1 AT3G26744 30.857 175 99 6 3 165 308 472 2.77e-13 67.4
MS.gene08514.t1 AT1G12860 30.909 165 97 5 3 158 268 424 2.86e-13 67.4
MS.gene08514.t1 AT3G26744 30.357 168 98 5 3 158 308 468 3.58e-13 67.4
MS.gene08514.t1 AT1G12860 31.677 161 93 5 3 154 268 420 3.67e-13 67.0
MS.gene08514.t1 AT2G16910 29.082 196 103 8 3 175 315 497 3.46e-12 64.3
MS.gene08514.t1 AT4G29930 29.630 162 95 5 3 161 55 200 4.37e-12 62.8
MS.gene08514.t1 AT5G57150 29.268 164 104 5 3 158 56 215 7.16e-12 62.0
MS.gene08514.t1 AT5G57150 28.743 167 107 5 3 161 55 217 1.00e-11 62.0

Find 26 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTGCTTTGGAAAATCTT+AGG 0.306717 8.3:+68204579 MS.gene08514:CDS
GATAAAGGTTATTGTTGCTT+TGG 0.325174 8.3:+68204568 MS.gene08514:CDS
GGTGACCAAGCATCAATAAT+TGG 0.351169 8.3:+68204293 MS.gene08514:CDS
ACTCAAAAGATGTGATGTTT+AGG 0.363568 8.3:-68204616 None:intergenic
ATGACCACCTTAGTGTTCTT+AGG 0.380570 8.3:+68203848 MS.gene08514:CDS
CACTAAGGTGGTCATTCATT+TGG 0.381725 8.3:-68203840 None:intergenic
CTCCTCCAATTATTGATGCT+TGG 0.405749 8.3:-68204298 None:intergenic
AAAGGCTGGTCAATTGATAA+AGG 0.417902 8.3:+68204553 MS.gene08514:CDS
TCATCATCACCGCCTAGTTA+TGG 0.434743 8.3:+68204413 MS.gene08514:CDS
ATATTCAGCTACATCAATAA+TGG 0.454301 8.3:+68205646 MS.gene08514:CDS
GAATAATACCTTGAGATTGA+AGG 0.480462 8.3:-68204654 None:intergenic
ATATTGCTGTTGAACGCAAT+AGG 0.480469 8.3:+68203812 MS.gene08514:CDS
TGCCATCTTCATATGTTCAA+AGG 0.494987 8.3:+68203878 MS.gene08514:CDS
GATAATTTCATTCCATAACT+AGG 0.502981 8.3:-68204425 None:intergenic
GCCATCTTCATATGTTCAAA+GGG 0.503887 8.3:+68203879 MS.gene08514:CDS
TTGTGAAGAAAATGAGGTAA+AGG 0.510975 8.3:+68204451 MS.gene08514:CDS
ACCCTTTGAACATATGAAGA+TGG 0.543479 8.3:-68203880 None:intergenic
ATCACCTTGTGAAGAAAATG+AGG 0.546176 8.3:+68204445 MS.gene08514:CDS
ATTGAAGAAGACTGTAAGCT+AGG 0.552236 8.3:+68205589 MS.gene08514:CDS
TGTGATGTTTAGGTGAAGAA+TGG 0.555251 8.3:-68204606 None:intergenic
ACTCTACTCCTTCAATCTCA+AGG 0.617899 8.3:+68204646 MS.gene08514:CDS
TGAGAGACCTAAGAACACTA+AGG 0.629812 8.3:-68203855 None:intergenic
GACCAAGCATCAATAATTGG+AGG 0.640978 8.3:+68204296 MS.gene08514:CDS
AGGCGGTGATGATGATGATG+AGG 0.686258 8.3:-68204405 None:intergenic
GAGACCTAAGAACACTAAGG+TGG 0.704555 8.3:-68203852 None:intergenic
AATTTCATTCCATAACTAGG+CGG 0.718157 8.3:-68204422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAATGATAAACAATGT+TGG - chr8.3:68204818-68204837 None:intergenic 15.0%
!!! ATAAAAAAGAATGAAGATTT+TGG + chr8.3:68204380-68204399 MS.gene08514:CDS 15.0%
!!! ATTTTTTTCATTTATGTGTA+TGG - chr8.3:68205148-68205167 None:intergenic 15.0%
!!! TTATGTTTTAAAAATCACTT+AGG + chr8.3:68204229-68204248 MS.gene08514:intron 15.0%
!! TATACAAAACCAAAAAAGAT+TGG - chr8.3:68204000-68204019 None:intergenic 20.0%
!! TGTTTAATGTTTATGTGATA+GGG + chr8.3:68204272-68204291 MS.gene08514:intron 20.0%
!! TTGTTTAATGTTTATGTGAT+AGG + chr8.3:68204271-68204290 MS.gene08514:intron 20.0%
!!! GTTAAAAATCCAATCTTTTT+TGG + chr8.3:68203988-68204007 MS.gene08514:intron 20.0%
!!! TTTTTTGGTTTTGTATACAA+GGG + chr8.3:68204003-68204022 MS.gene08514:intron 20.0%
! AAATAACGAGCTGTATATAT+GGG + chr8.3:68205558-68205577 MS.gene08514:intron 25.0%
! AAGATTAGAGAAATAAGAAG+TGG - chr8.3:68205029-68205048 None:intergenic 25.0%
! ACATGAAAATCTCAAAAGAA+AGG - chr8.3:68203970-68203989 None:intergenic 25.0%
! ATAGAACACACTATTTGAAA+GGG + chr8.3:68205062-68205081 MS.gene08514:intron 25.0%
! ATATTCAGCTACATCAATAA+TGG + chr8.3:68205646-68205665 MS.gene08514:CDS 25.0%
! GAAAATTGAGTGCAAAAAAA+AGG + chr8.3:68204535-68204554 MS.gene08514:CDS 25.0%
! GATAATTTCATTCCATAACT+AGG - chr8.3:68204428-68204447 None:intergenic 25.0%
! TAAATAACGAGCTGTATATA+TGG + chr8.3:68205535-68205554 MS.gene08514:intron 25.0%
!!! AAGAAAGGATTTTGAAGAAA+AGG - chr8.3:68203955-68203974 None:intergenic 25.0%
!!! AATTGATTTTGTGAAAGAGT+TGG + chr8.3:68204322-68204341 MS.gene08514:CDS 25.0%
!!! AGAAAGGATTTTGAAGAAAA+GGG - chr8.3:68203954-68203973 None:intergenic 25.0%
!!! CTTTTTTGGTTTTGTATACA+AGG + chr8.3:68204002-68204021 MS.gene08514:intron 25.0%
AATCTCTTGAAGCACAAAAA+AGG + chr8.3:68204357-68204376 MS.gene08514:CDS 30.0%
AATTTCATTCCATAACTAGG+CGG - chr8.3:68204425-68204444 None:intergenic 30.0%
ACTCAAAAGATGTGATGTTT+AGG - chr8.3:68204619-68204638 None:intergenic 30.0%
ACTCTAATGTTGAGTTTATG+AGG + chr8.3:68205293-68205312 MS.gene08514:intron 30.0%
CATAGAACACACTATTTGAA+AGG + chr8.3:68205061-68205080 MS.gene08514:intron 30.0%
GAATAATACCTTGAGATTGA+AGG - chr8.3:68204657-68204676 None:intergenic 30.0%
TAGAACACACTATTTGAAAG+GGG + chr8.3:68205063-68205082 MS.gene08514:intron 30.0%
TTGTGAAGAAAATGAGGTAA+AGG + chr8.3:68204451-68204470 MS.gene08514:CDS 30.0%
! GATAAAGGTTATTGTTGCTT+TGG + chr8.3:68204568-68204587 MS.gene08514:CDS 30.0%
! TTTACCTCATTTTCTTCACA+AGG - chr8.3:68204452-68204471 None:intergenic 30.0%
!! TTCAGCTAGTTTCTAGATTT+TGG + chr8.3:68205363-68205382 MS.gene08514:intron 30.0%
!! TTGTTGCTTTGGAAAATCTT+AGG + chr8.3:68204579-68204598 MS.gene08514:CDS 30.0%
!!! ACTCAATTTTCAAGTTCACA+TGG - chr8.3:68204526-68204545 None:intergenic 30.0%
!!! CTCAATTTTCAAGTTCACAT+GGG - chr8.3:68204525-68204544 None:intergenic 30.0%
ACCCTTTGAACATATGAAGA+TGG - chr8.3:68203883-68203902 None:intergenic 35.0%
AGCTAATGCTATGAGTATGA+GGG + chr8.3:68204939-68204958 MS.gene08514:intron 35.0%
AGCTCATAGATTAACTAAGG+TGG + chr8.3:68205333-68205352 MS.gene08514:intron 35.0%
ATCACCTTGTGAAGAAAATG+AGG + chr8.3:68204445-68204464 MS.gene08514:CDS 35.0%
ATTGAAGAAGACTGTAAGCT+AGG + chr8.3:68205589-68205608 MS.gene08514:CDS 35.0%
ATTGAGTGCAAAAAAAAGGC+TGG + chr8.3:68204539-68204558 MS.gene08514:CDS 35.0%
GATAAACAATGTTGGAACCA+AGG - chr8.3:68204810-68204829 None:intergenic 35.0%
GCCATCTTCATATGTTCAAA+GGG + chr8.3:68203879-68203898 MS.gene08514:CDS 35.0%
TAAATGTGGTCAACTCACTA+TGG + chr8.3:68204729-68204748 MS.gene08514:intron 35.0%
TGCCATCTTCATATGTTCAA+AGG + chr8.3:68203878-68203897 MS.gene08514:CDS 35.0%
TGTGATGTTTAGGTGAAGAA+TGG - chr8.3:68204609-68204628 None:intergenic 35.0%
! AAAGGCTGGTCAATTGATAA+AGG + chr8.3:68204553-68204572 MS.gene08514:CDS 35.0%
! ATATTGCTGTTGAACGCAAT+AGG + chr8.3:68203812-68203831 MS.gene08514:CDS 35.0%
! TTTGTATACAAGGGCATGTT+TGG + chr8.3:68204012-68204031 MS.gene08514:intron 35.0%
AAGAGTTGAGTTTGTCCACA+AGG + chr8.3:68204906-68204925 MS.gene08514:intron 40.0%
ACTCTACTCCTTCAATCTCA+AGG + chr8.3:68204646-68204665 MS.gene08514:CDS 40.0%
ATGACCACCTTAGTGTTCTT+AGG + chr8.3:68203848-68203867 MS.gene08514:CDS 40.0%
CACTAAGGTGGTCATTCATT+TGG - chr8.3:68203843-68203862 None:intergenic 40.0%
CTCCTCCAATTATTGATGCT+TGG - chr8.3:68204301-68204320 None:intergenic 40.0%
GACCAAGCATCAATAATTGG+AGG + chr8.3:68204296-68204315 MS.gene08514:CDS 40.0%
GAGCTAATGCTATGAGTATG+AGG + chr8.3:68204938-68204957 MS.gene08514:intron 40.0%
GGCAGCTCATAGATTAACTA+AGG + chr8.3:68205330-68205349 MS.gene08514:intron 40.0%
GGTGACCAAGCATCAATAAT+TGG + chr8.3:68204293-68204312 MS.gene08514:CDS 40.0%
TATGAGGAGACAAGCAAATG+AGG + chr8.3:68205309-68205328 MS.gene08514:intron 40.0%
TGAGAGACCTAAGAACACTA+AGG - chr8.3:68203858-68203877 None:intergenic 40.0%
TTTGTCCACAAGGTAAAAGC+TGG + chr8.3:68204916-68204935 MS.gene08514:intron 40.0%
! CTTGCTATCTAAGCAAAACC+TGG + chr8.3:68204781-68204800 MS.gene08514:intron 40.0%
GAGACCTAAGAACACTAAGG+TGG - chr8.3:68203855-68203874 None:intergenic 45.0%
TAAGCAAAACCTGGCTTCCT+TGG + chr8.3:68204790-68204809 MS.gene08514:intron 45.0%
TCATCATCACCGCCTAGTTA+TGG + chr8.3:68204413-68204432 MS.gene08514:CDS 45.0%
! TAGCTCCAGCTTTTACCTTG+TGG - chr8.3:68204924-68204943 None:intergenic 45.0%
!! TTAAACTAAAATAATTTAAT+CGG + chr8.3:68205167-68205186 MS.gene08514:intron 5.0%
!!! AAATTTACTAATTTTAAATA+TGG - chr8.3:68204872-68204891 None:intergenic 5.0%
!!! TTATTTTATTTTTTTAAATG+TGG + chr8.3:68204715-68204734 MS.gene08514:intron 5.0%
ATGTTGGAACCAAGGAAGCC+AGG - chr8.3:68204802-68204821 None:intergenic 50.0%
! AGGCGGTGATGATGATGATG+AGG - chr8.3:68204408-68204427 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 68203805 68205675 68203805 ID=MS.gene08514
chr8.3 mRNA 68203805 68205675 68203805 ID=MS.gene08514.t1;Parent=MS.gene08514
chr8.3 exon 68203805 68203900 68203805 ID=MS.gene08514.t1.exon1;Parent=MS.gene08514.t1
chr8.3 CDS 68203805 68203900 68203805 ID=cds.MS.gene08514.t1;Parent=MS.gene08514.t1
chr8.3 exon 68204293 68204667 68204293 ID=MS.gene08514.t1.exon2;Parent=MS.gene08514.t1
chr8.3 CDS 68204293 68204667 68204293 ID=cds.MS.gene08514.t1;Parent=MS.gene08514.t1
chr8.3 exon 68205589 68205675 68205589 ID=MS.gene08514.t1.exon3;Parent=MS.gene08514.t1
chr8.3 CDS 68205589 68205675 68205589 ID=cds.MS.gene08514.t1;Parent=MS.gene08514.t1
Gene Sequence

>MS.gene08514

ATGACACATATTGCTGTTGAACGCAATAGGAGACGCCAAATGAATGACCACCTTAGTGTTCTTAGGTCTCTCATGCCATCTTCATATGTTCAAAGGGTATGAACTTTTTAATCATTATTTATGAAATTTGATGTCAAATTCTTATACCCTTTTCTTCAAAATCCTTTCTTTTGAGATTTTCATGTTAAAAATCCAATCTTTTTTGGTTTTGTATACAAGGGCATGTTTGGATTTTCTTTGAGTTTGACATAATCACATTATGTTAATGATATTTTAGTAAAAATTAATTTTTTAGCTTCAATAAAATGTTAAGTGTCTAGTGATTTTTTCAATTTGTTTTTGTGTTAATTTTGTCAAACTCATTTTGTTAAATTTAAACTCTATTTAAATATACACTAAATTTTTTGTTTTTTTATTTATTTTTTTATGTTTTAAAAATCACTTAGGTAAAGATTTTTTTTTTGATTTGTTTAATGTTTATGTGATAGGGTGACCAAGCATCAATAATTGGAGGAGCAATTGATTTTGTGAAAGAGTTGGAACAATTGCTTGAATCTCTTGAAGCACAAAAAAGGATAAAAAAGAATGAAGATTTTGGTTCCTCATCATCATCATCACCGCCTAGTTATGGAATGAAATTATCACCTTGTGAAGAAAATGAGGTAAAGGCAGAAAATAAATCAGAAGCAGCAGATATAAAAGTAACATTGATTCAAACCCATGTGAACTTGAAAATTGAGTGCAAAAAAAAGGCTGGTCAATTGATAAAGGTTATTGTTGCTTTGGAAAATCTTAGGCTAACCATTCTTCACCTAAACATCACATCTTTTGAGTCTTCTGTACTCTACTCCTTCAATCTCAAGGTATTATTCTGTCTTTTCACTTATCATAGTGTTTTCTTTTTAATTTTTTATTTTATTTTTTTAAATGTGGTCAACTCACTATGGTATCAAGTCAGAGTACTGTTCTTTTCTGACTTGCTATCTAAGCAAAACCTGGCTTCCTTGGTTCCAACATTGTTTATCATTATAAAATTAAATAAAATGCATTTTGATAAATTATTACCATATTTAAAATTAGTAAATTTATATATATGTTAAGAAGAGTTGAGTTTGTCCACAAGGTAAAAGCTGGAGCTAATGCTATGAGTATGAGGGCACAGAGTTCAAATTTTCTTAAAAACTAATATTAAATAGTTTTTAATGTTACTTTAATTAATAGCCACTTCTTATTTCTCTAATCTTGTTTCTAGAACACATAGAACACACTATTTGAAAGGGGAACTATGATCATTGTTGCAAGTTTTATTTGCTCACAACACTTGATAAAAAAATATTTAGCCATACACATAAATGAAAAAAATTAAACTAAAATAATTTAATCGGATCATTTAATTATTTGAACTATTTATTCATCTGCACATCAAGATATTACATAATTATGACAATTATTATAATTGTAAAATTATCTTTAAACTATATTATGTCAACTCTAATGTTGAGTTTATGAGGAGACAAGCAAATGAGGCAGCTCATAGATTAACTAAGGTGGTCACATCTTCAGCTAGTTTCTAGATTTTGGTAGAAATATCATATTGTATTAAACACATTTTAAGTAATGAAATGATACAAGCTTCTTTCTTTAAAAAAACTTTTTATAATTTTTTTATTAGTTGTTTTTGACAAATTTTCTAAATTTTTTATTAGTACTATTTATTATCATTTGATAAATAAATAACGAGCTGTATATAAATAAATAACGAGCTGTATATATGGGTGATACAGATTGAAGAAGACTGTAAGCTAGGCACAGCAAATGACATAGCAGAAGCAGTTCATCAAATATTCAGCTACATCAATAATGGTAGCTAA

Protein sequence

>MS.gene08514.t1

MTHIAVERNRRRQMNDHLSVLRSLMPSSYVQRGDQASIIGGAIDFVKELEQLLESLEAQKRIKKNEDFGSSSSSSPPSYGMKLSPCEENEVKAENKSEAADIKVTLIQTHVNLKIECKKKAGQLIKVIVALENLRLTILHLNITSFESSVLYSFNLKIEEDCKLGTANDIAEAVHQIFSYINNGS