AlfalfaGEDB | MS.gene09740

M. sativa cultivar XinJiangDaYe / MS.gene09740

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09740.t1	RHN53781.1	84.1	107	15	1	7	111	34	140	9.60E-41	176

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09740.t1	A0A396HMR3	84.1	107	15	1	7	111	34	140	6.9e-41	176.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene09740	0.811081	8.37E-51	-1.69E-46
MS.gene049272	MS.gene09740	0.816701	4.83E-52	-1.69E-46
MS.gene049343	MS.gene09740	0.817418	3.33E-52	-1.69E-46
MS.gene049850	MS.gene09740	0.812133	4.94E-51	-1.69E-46
MS.gene050137	MS.gene09740	0.801609	8.31E-49	-1.69E-46
MS.gene050156	MS.gene09740	0.990547	3.72E-183	-1.69E-46
MS.gene05045	MS.gene09740	0.806239	9.06E-50	-1.69E-46
MS.gene050844	MS.gene09740	0.845964	2.92E-59	-1.69E-46
MS.gene051247	MS.gene09740	0.806686	7.29E-50	-1.69E-46
MS.gene051719	MS.gene09740	0.800631	1.32E-48	-1.69E-46
MS.gene052042	MS.gene09740	0.811694	6.16E-51	-1.69E-46
MS.gene052519	MS.gene09740	0.835168	1.96E-56	-1.69E-46
MS.gene052611	MS.gene09740	0.835968	1.23E-56	-1.69E-46
MS.gene052773	MS.gene09740	0.834538	2.83E-56	-1.69E-46
MS.gene052863	MS.gene09740	0.844154	9.01E-59	-1.69E-46
MS.gene052896	MS.gene09740	0.810035	1.41E-50	-1.69E-46
MS.gene053145	MS.gene09740	0.805238	1.47E-49	-1.69E-46
MS.gene053226	MS.gene09740	0.824967	6.06E-54	-1.69E-46
MS.gene053966	MS.gene09740	0.815093	1.10E-51	-1.69E-46
MS.gene054467	MS.gene09740	0.813953	1.97E-51	-1.69E-46
MS.gene054468	MS.gene09740	0.806755	7.05E-50	-1.69E-46
MS.gene054472	MS.gene09740	0.810096	1.37E-50	-1.69E-46
MS.gene054651	MS.gene09740	0.805838	1.10E-49	-1.69E-46
MS.gene054775	MS.gene09740	0.802345	5.86E-49	-1.69E-46
MS.gene055357	MS.gene09740	0.806529	7.87E-50	-1.69E-46
MS.gene055818	MS.gene09740	0.869541	2.87E-66	-1.69E-46
MS.gene056050	MS.gene09740	0.830974	2.17E-55	-1.69E-46
MS.gene056173	MS.gene09740	0.808851	2.53E-50	-1.69E-46
MS.gene056366	MS.gene09740	0.808235	3.42E-50	-1.69E-46
MS.gene056373	MS.gene09740	0.802494	5.46E-49	-1.69E-46
MS.gene056595	MS.gene09740	0.832929	7.14E-56	-1.69E-46
MS.gene057026	MS.gene09740	0.839535	1.50E-57	-1.69E-46
MS.gene057444	MS.gene09740	0.804694	1.91E-49	-1.69E-46
MS.gene057855	MS.gene09740	0.801868	7.35E-49	-1.69E-46
MS.gene057915	MS.gene09740	0.806346	8.60E-50	-1.69E-46
MS.gene058502	MS.gene09740	0.800752	1.24E-48	-1.69E-46
MS.gene058625	MS.gene09740	0.841346	5.02E-58	-1.69E-46
MS.gene058636	MS.gene09740	0.831171	1.94E-55	-1.69E-46
MS.gene058792	MS.gene09740	0.808736	2.67E-50	-1.69E-46
MS.gene058829	MS.gene09740	0.8438	1.12E-58	-1.69E-46
MS.gene058979	MS.gene09740	0.805384	1.37E-49	-1.69E-46
MS.gene059272	MS.gene09740	0.814182	1.76E-51	-1.69E-46
MS.gene059570	MS.gene09740	0.822665	2.10E-53	-1.69E-46
MS.gene060322	MS.gene09740	0.815699	8.09E-52	-1.69E-46
MS.gene060572	MS.gene09740	0.823454	1.37E-53	-1.69E-46
MS.gene060781	MS.gene09740	0.81241	4.30E-51	-1.69E-46
MS.gene060803	MS.gene09740	0.80826	3.38E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09740.t1	MTR_5g013080	83.178	107	16	1	7	111	34	140	3.05e-56	173
MS.gene09740.t1	MTR_5g013140	69.524	105	32	0	7	111	147	251	8.42e-40	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAATGAGACCTACAAAAC+TGG	0.420267	5.4:-6977109	MS.gene09740:CDS
GCAATCATGGAAGTCCTGTT+TGG	0.422527	5.4:-6977173	MS.gene09740:CDS
TATTGAGAATACTTTAAATC+CGG	0.432788	5.4:-6977277	MS.gene09740:CDS
TTGTGTTGGACACAAACTTT+CGG	0.442750	5.4:+6977756	None:intergenic
GTTCACTGTCTTCTTATACT+TGG	0.447231	5.4:+6977197	None:intergenic
AGAAGCATGCTTAACATCAT+TGG	0.458517	5.4:-6977145	MS.gene09740:CDS
GCAGCCATACATGCAACATA+AGG	0.463012	5.4:-6977244	MS.gene09740:CDS
AAGACAGTGAACTGCAATCA+TGG	0.467236	5.4:-6977186	MS.gene09740:CDS
CATGCTTCTCCATTCCAAAC+AGG	0.474983	5.4:+6977159	None:intergenic
TGGCAGGGCGATGGTAGTGC+TGG	0.481700	5.4:-6977318	MS.gene09740:intron
AGTGCCTTATGTTGCATGTA+TGG	0.483200	5.4:+6977240	None:intergenic
ACTGTCTTCTTATACTTGGT+CGG	0.489810	5.4:+6977201	None:intergenic
TCGGTTGCACATTCTCGAAC+CGG	0.514678	5.4:+6977775	None:intergenic
CATGGAAGTCCTGTTTGGAA+TGG	0.547429	5.4:-6977168	MS.gene09740:CDS
GTGGAGGTAAATAAAGATGA+AGG	0.554468	5.4:-6977798	MS.gene09740:CDS
TATCAAAAGAGCTATTGTGT+TGG	0.555066	5.4:+6977742	None:intergenic
GGTGGACAATGGCAGGGCGA+TGG	0.604468	5.4:-6977327	MS.gene09740:intron
TTGCATAATGAGGGAAACAG+TGG	0.626434	5.4:-6977817	None:intergenic
CATAATGAGGGAAACAGTGG+AGG	0.636407	5.4:-6977814	None:intergenic
AGGTAAATAAAGATGAAGGC+CGG	0.649787	5.4:-6977794	MS.gene09740:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGATTAGTAATTTGAAT+TGG	-	chr5.4:6977252-6977271	MS.gene09740:CDS	15.0%
!!	TATTGAGAATACTTTAAATC+CGG	-	chr5.4:6977634-6977653	MS.gene09740:intron	20.0%
!!!	TAACTGAATGATCTATTTTT+AGG	+	chr5.4:6977443-6977462	None:intergenic	20.0%
!	AAGATGCATAATCAAAATTC+AGG	+	chr5.4:6977293-6977312	None:intergenic	25.0%
!	GATTATGCATCTTAGTAATA+TGG	-	chr5.4:6977300-6977319	MS.gene09740:CDS	25.0%
!	TGAATGGAAATTGTTTAGTT+TGG	-	chr5.4:6977380-6977399	MS.gene09740:intron	25.0%
!!	TAGCTCTTTTGATAAAAACA+TGG	-	chr5.4:6977178-6977197	MS.gene09740:CDS	25.0%
	GAGATATTACAAGAGAAATC+AGG	+	chr5.4:6977220-6977239	None:intergenic	30.0%
	TATCAAAAGAGCTATTGTGT+TGG	+	chr5.4:6977172-6977191	None:intergenic	30.0%
!	CTCTTTTGATAAAAACATGG+AGG	-	chr5.4:6977181-6977200	MS.gene09740:CDS	30.0%
!!	TAGTTAGTTGATGACATGAA+TGG	-	chr5.4:6977364-6977383	MS.gene09740:intron	30.0%
!!	TTTAGTTTGGCAAACTTCAA+AGG	-	chr5.4:6977393-6977412	MS.gene09740:intron	30.0%
	AACAGTGAGCTCAAATTACT+TGG	+	chr5.4:6977557-6977576	None:intergenic	35.0%
	AACTTTCTTCTGTTGAACGT+TGG	+	chr5.4:6977498-6977517	None:intergenic	35.0%
	ACAGTGAGCTCAAATTACTT+GGG	+	chr5.4:6977556-6977575	None:intergenic	35.0%
	ACTGTCTTCTTATACTTGGT+CGG	+	chr5.4:6977713-6977732	None:intergenic	35.0%
	AGGTAAATAAAGATGAAGGC+CGG	-	chr5.4:6977117-6977136	MS.gene09740:CDS	35.0%
	CAGTTAAGTGATGACATGAA+TGG	-	chr5.4:6977457-6977476	MS.gene09740:intron	35.0%
	CTAACTAATGACCTACTTCA+AGG	+	chr5.4:6977351-6977370	None:intergenic	35.0%
	GTGGAGGTAAATAAAGATGA+AGG	-	chr5.4:6977113-6977132	MS.gene09740:CDS	35.0%
	GTTCACTGTCTTCTTATACT+TGG	+	chr5.4:6977717-6977736	None:intergenic	35.0%
	TTGTGTTGGACACAAACTTT+CGG	+	chr5.4:6977158-6977177	None:intergenic	35.0%
!	AGAAGCATGCTTAACATCAT+TGG	-	chr5.4:6977766-6977785	MS.gene09740:CDS	35.0%
!	TATGGCTGCAAAATCTTTTC+CGG	+	chr5.4:6977656-6977675	None:intergenic	35.0%
	AAGACAGTGAACTGCAATCA+TGG	-	chr5.4:6977725-6977744	MS.gene09740:intron	40.0%
	AGTGCCTTATGTTGCATGTA+TGG	+	chr5.4:6977674-6977693	None:intergenic	40.0%
	TGCAATGAGACCTACAAAAC+TGG	-	chr5.4:6977802-6977821	MS.gene09740:CDS	40.0%
!!	TTGAGCTCACTGTTTTTCTG+TGG	-	chr5.4:6977563-6977582	MS.gene09740:intron	40.0%
!!!	TGTTTTTCTGTGGTGGACAA+TGG	-	chr5.4:6977573-6977592	MS.gene09740:intron	40.0%
	CATGCTTCTCCATTCCAAAC+AGG	+	chr5.4:6977755-6977774	None:intergenic	45.0%
	CATGGAAGTCCTGTTTGGAA+TGG	-	chr5.4:6977743-6977762	MS.gene09740:CDS	45.0%
	GCAATCATGGAAGTCCTGTT+TGG	-	chr5.4:6977738-6977757	MS.gene09740:CDS	45.0%
	GCAGCCATACATGCAACATA+AGG	-	chr5.4:6977667-6977686	MS.gene09740:intron	45.0%
	GCTCAATTGCACCTTGAAGT+AGG	-	chr5.4:6977337-6977356	MS.gene09740:intron	45.0%
!!	AGCTCACTGTTTTTCTGTGG+TGG	-	chr5.4:6977566-6977585	MS.gene09740:intron	45.0%
	TCGGTTGCACATTCTCGAAC+CGG	+	chr5.4:6977139-6977158	None:intergenic	50.0%
!!	TTCTGTGGTGGACAATGGCA+GGG	-	chr5.4:6977578-6977597	MS.gene09740:intron	50.0%
!!	TTTCTGTGGTGGACAATGGC+AGG	-	chr5.4:6977577-6977596	MS.gene09740:intron	50.0%
	GGTGGACAATGGCAGGGCGA+TGG	-	chr5.4:6977584-6977603	MS.gene09740:intron	65.0%
!	TGGCAGGGCGATGGTAGTGC+TGG	-	chr5.4:6977593-6977612	MS.gene09740:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	6977101	6977832	6977101	ID=MS.gene09740
chr5.4	mRNA	6977101	6977832	6977101	ID=MS.gene09740.t1;Parent=MS.gene09740
chr5.4	exon	6977731	6977832	6977731	ID=MS.gene09740.t1.exon1;Parent=MS.gene09740.t1
chr5.4	CDS	6977731	6977832	6977731	ID=cds.MS.gene09740.t1;Parent=MS.gene09740.t1
chr5.4	exon	6977101	6977334	6977101	ID=MS.gene09740.t1.exon2;Parent=MS.gene09740.t1
chr5.4	CDS	6977101	6977334	6977101	ID=cds.MS.gene09740.t1;Parent=MS.gene09740.t1

Gene Sequence

>MS.gene09740

ATGAGGGAAACAGTGGAGGTAAATAAAGATGAAGGCCGGTTCGAGAATGTGCAACCGAAAGTTTGTGTCCAACACAATAGCTCTTTTGATAAAAACATGGAGGTAAAGTGAAAATTCCTGATTTCTCTTGTAATATCTCTGTGATAGAATTAATTGATTAGTAATTTGAATTGGTTAATGATCAATGTTCCTGAATTTTGATTATGCATCTTAGTAATATGGCAATAAACATTTATGCTCAATTGCACCTTGAAGTAGGTCATTAGTTAGTTGATGACATGAATGGAAATTGTTTAGTTTGGCAAACTTCAAAGGTGAACAGTTATGCTCTCATTTGCACCTAAAAATAGATCATTCAGTTAAGTGATGACATGAATGGATACTGTTTTCTTTGCCAACGTTCAACAGAAGAAAGTTTGATTAATAGATGACACAAAATACATGAATTTGTTCCCAAGTAATTTGAGCTCACTGTTTTTCTGTGGTGGACAATGGCAGGGCGATGGTAGTGCTGGAATGAAGAAAATTTTAACTATTGAGAATACTTTAAATCCGGAAAAGATTTTGCAGCCATACATGCAACATAAGGCACTTCATCATACTCTTGATCCGACCAAGTATAAGAAGACAGTGAACTGCAATCATGGAAGTCCTGTTTGGAATGGAGAAGCATGCTTAACATCATTGGACACTGATACAAGTGCAATGAGACCTACAAAACTGGTGCAATGA

Protein sequence

>MS.gene09740.t1

MRETVEVNKDEGRFENVQPKVCVQHNSSFDKNMEGDGSAGMKKILTIENTLNPEKILQPYMQHKALHHTLDPTKYKKTVNCNHGSPVWNGEACLTSLDTDTSAMRPTKLVQ