Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20988.t1 | AES61490.1 | 88.9 | 108 | 8 | 3 | 1 | 106 | 1 | 106 | 4.80E-42 | 180.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20988.t1 | G7IAA9 | 88.9 | 108 | 8 | 3 | 1 | 106 | 1 | 106 | 3.5e-42 | 180.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene20988 | 0.815545 | 8.75E-52 | -1.69E-46 |
| MS.gene049681 | MS.gene20988 | 0.801042 | 1.09E-48 | -1.69E-46 |
| MS.gene049758 | MS.gene20988 | 0.802362 | 5.82E-49 | -1.69E-46 |
| MS.gene050128 | MS.gene20988 | 0.816368 | 5.73E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene20988 | 0.826537 | 2.57E-54 | -1.69E-46 |
| MS.gene050558 | MS.gene20988 | 0.800013 | 1.76E-48 | -1.69E-46 |
| MS.gene051487 | MS.gene20988 | 0.800891 | 1.16E-48 | -1.69E-46 |
| MS.gene051663 | MS.gene20988 | 0.804017 | 2.64E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene20988 | 0.815147 | 1.07E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene20988 | 0.824973 | 6.04E-54 | -1.69E-46 |
| MS.gene052178 | MS.gene20988 | 0.832863 | 7.41E-56 | -1.69E-46 |
| MS.gene052207 | MS.gene20988 | 0.820938 | 5.26E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene20988 | 0.834959 | 2.22E-56 | -1.69E-46 |
| MS.gene053204 | MS.gene20988 | 0.814203 | 1.74E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene20988 | 0.840147 | 1.04E-57 | -1.69E-46 |
| MS.gene054010 | MS.gene20988 | 0.860656 | 1.77E-63 | -1.69E-46 |
| MS.gene054011 | MS.gene20988 | 0.872244 | 3.69E-67 | -1.69E-46 |
| MS.gene054012 | MS.gene20988 | 0.872808 | 2.39E-67 | -1.69E-46 |
| MS.gene054013 | MS.gene20988 | 0.875013 | 4.31E-68 | -1.69E-46 |
| MS.gene054214 | MS.gene20988 | 0.865693 | 4.91E-65 | -1.69E-46 |
| MS.gene054473 | MS.gene20988 | 0.822252 | 2.62E-53 | -1.69E-46 |
| MS.gene05456 | MS.gene20988 | 0.803164 | 3.97E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene20988 | 0.827915 | 1.20E-54 | -1.69E-46 |
| MS.gene054618 | MS.gene20988 | 0.811246 | 7.71E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene20988 | 0.808621 | 2.83E-50 | -1.69E-46 |
| MS.gene055303 | MS.gene20988 | 0.827304 | 1.68E-54 | -1.69E-46 |
| MS.gene055358 | MS.gene20988 | 0.800854 | 1.19E-48 | -1.69E-46 |
| MS.gene055390 | MS.gene20988 | 0.811884 | 5.60E-51 | -1.69E-46 |
| MS.gene055422 | MS.gene20988 | 0.808601 | 2.86E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene20988 | 0.807149 | 5.82E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene20988 | 0.85593 | 4.53E-62 | -1.69E-46 |
| MS.gene056191 | MS.gene20988 | 0.807913 | 4.01E-50 | -1.69E-46 |
| MS.gene056772 | MS.gene20988 | 0.813707 | 2.23E-51 | -1.69E-46 |
| MS.gene056902 | MS.gene20988 | 0.828179 | 1.04E-54 | -1.69E-46 |
| MS.gene057121 | MS.gene20988 | 0.822683 | 2.08E-53 | -1.69E-46 |
| MS.gene057469 | MS.gene20988 | 0.820914 | 5.33E-53 | -1.69E-46 |
| MS.gene05811 | MS.gene20988 | 0.821766 | 3.39E-53 | -1.69E-46 |
| MS.gene058168 | MS.gene20988 | 0.807564 | 4.75E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene20988 | 0.824944 | 6.13E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene20988 | 0.817315 | 3.52E-52 | -1.69E-46 |
| MS.gene059228 | MS.gene20988 | 0.806857 | 6.71E-50 | -1.69E-46 |
| MS.gene059294 | MS.gene20988 | 0.822001 | 2.99E-53 | -1.69E-46 |
| MS.gene06037 | MS.gene20988 | 0.819616 | 1.06E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene20988 | 0.829765 | 4.29E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20988.t1 | MTR_1g084780 | 86.111 | 108 | 11 | 3 | 1 | 106 | 1 | 106 | 2.19e-49 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCCATGTTGAAAGTTTC+AGG | 0.145284 | 1.3:-60130405 | None:intergenic |
| ACTATGGTCCTGATGGTTTA+AGG | 0.152607 | 1.3:+60130478 | MS.gene20988:CDS |
| CCTGGGACTCTTGGTGGAAT+TGG | 0.298107 | 1.3:+60130552 | MS.gene20988:CDS |
| AGCAAAGTTGCCAGGGCTGC+TGG | 0.302890 | 1.3:-60130445 | None:intergenic |
| CCTACTATCCCTGGGACTCT+TGG | 0.340847 | 1.3:+60130543 | MS.gene20988:CDS |
| TGCATTATGTTGGTTGTCCT+TGG | 0.369781 | 1.3:+60130327 | MS.gene20988:CDS |
| GGTGGAATTGGAAATGGAAT+TGG | 0.405856 | 1.3:+60130564 | MS.gene20988:CDS |
| AGCCCTGGCAACTTTGCTGC+AGG | 0.406785 | 1.3:+60130450 | MS.gene20988:CDS |
| ACTCTTGGTGGAATTGGAAA+TGG | 0.433410 | 1.3:+60130558 | MS.gene20988:CDS |
| TTCAACATGGGAGGAGGGAA+TGG | 0.438827 | 1.3:+60130414 | MS.gene20988:CDS |
| CCAAGAGTCCCAGGGATAGT+AGG | 0.443589 | 1.3:-60130543 | None:intergenic |
| ACTATCCCTGGGACTCTTGG+TGG | 0.449895 | 1.3:+60130546 | MS.gene20988:CDS |
| GGTGATGTTGTGCATTATGT+TGG | 0.453491 | 1.3:+60130317 | MS.gene20988:CDS |
| ACACCTGCAGCAAAGTTGCC+AGG | 0.460348 | 1.3:-60130453 | None:intergenic |
| CCAATTCCACCAAGAGTCCC+AGG | 0.481288 | 1.3:-60130552 | None:intergenic |
| TTTGCTGCAGGTGTAAACTA+TGG | 0.486819 | 1.3:+60130462 | MS.gene20988:CDS |
| TCAGTTCATGTGGAAGGAGC+TGG | 0.487807 | 1.3:+60130351 | MS.gene20988:CDS |
| TTTGTTGTATTCTATGGATG+TGG | 0.489015 | 1.3:-60130603 | None:intergenic |
| GCAAAGTTGCCAGGGCTGCT+GGG | 0.489518 | 1.3:-60130444 | None:intergenic |
| TTCCCTGGTGGCTGTACCAG+TGG | 0.504867 | 1.3:+60130510 | MS.gene20988:CDS |
| ATTGGAAATGGAATTGGAGC+TGG | 0.505279 | 1.3:+60130570 | MS.gene20988:CDS |
| TCAACATGGGAGGAGGGAAT+GGG | 0.507559 | 1.3:+60130415 | MS.gene20988:CDS |
| TTGCCACTGGTACAGCCACC+AGG | 0.516455 | 1.3:-60130513 | None:intergenic |
| CTTGGTTCAGTTCATGTGGA+AGG | 0.517140 | 1.3:+60130345 | MS.gene20988:CDS |
| GAAACTTTCAACATGGGAGG+AGG | 0.522497 | 1.3:+60130408 | MS.gene20988:CDS |
| GTGCAAAACCTTAAACCATC+AGG | 0.527769 | 1.3:-60130486 | None:intergenic |
| GGGATACTTCCCAGCAGCCC+TGG | 0.530178 | 1.3:+60130435 | MS.gene20988:CDS |
| GTTGTATTCTATGGATGTGG+TGG | 0.547296 | 1.3:-60130600 | None:intergenic |
| CACCTGCAGCAAAGTTGCCA+GGG | 0.561342 | 1.3:-60130452 | None:intergenic |
| AGCTGGAAGGTTGCTGAAGG+AGG | 0.566335 | 1.3:+60130368 | MS.gene20988:CDS |
| ACTGGTACAGCCACCAGGGA+AGG | 0.569138 | 1.3:-60130508 | None:intergenic |
| TGTCCTTGGTTCAGTTCATG+TGG | 0.588978 | 1.3:+60130341 | MS.gene20988:CDS |
| GGTGAGAGTTGCAAAGGTGT+TGG | 0.597999 | 1.3:+60130296 | MS.gene20988:CDS |
| ACATCCTGAAACTTTCAACA+TGG | 0.599933 | 1.3:+60130401 | MS.gene20988:CDS |
| AAACTTTCAACATGGGAGGA+GGG | 0.601619 | 1.3:+60130409 | MS.gene20988:CDS |
| AGTAGGAAAAGTATTGCCAC+TGG | 0.619129 | 1.3:-60130526 | None:intergenic |
| GGTGTAAACTATGGTCCTGA+TGG | 0.623829 | 1.3:+60130471 | MS.gene20988:CDS |
| CATCCTGAAACTTTCAACAT+GGG | 0.624107 | 1.3:+60130402 | MS.gene20988:CDS |
| AGGAGCTGGAAGGTTGCTGA+AGG | 0.626981 | 1.3:+60130365 | MS.gene20988:CDS |
| CAATTCCACCAAGAGTCCCA+GGG | 0.630518 | 1.3:-60130551 | None:intergenic |
| CCTGAAACTTTCAACATGGG+AGG | 0.659348 | 1.3:+60130405 | MS.gene20988:CDS |
| CTTCCACATGAACTGAACCA+AGG | 0.677096 | 1.3:-60130344 | None:intergenic |
| TGCCACTGGTACAGCCACCA+GGG | 0.681445 | 1.3:-60130512 | None:intergenic |
| TTCATGTGGAAGGAGCTGGA+AGG | 0.707170 | 1.3:+60130355 | MS.gene20988:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATCCTGAAACTTTCAACA+TGG | + | chr1.3:60130401-60130420 | MS.gene20988:CDS | 35.0% | |
| CATCCTGAAACTTTCAACAT+GGG | + | chr1.3:60130402-60130421 | MS.gene20988:CDS | 35.0% | |
| ! | AATACTTTTCCTACTATCCC+TGG | + | chr1.3:60130534-60130553 | MS.gene20988:CDS | 35.0% |
| ! | ATACTTTTCCTACTATCCCT+GGG | + | chr1.3:60130535-60130554 | MS.gene20988:CDS | 35.0% |
| AAACTTTCAACATGGGAGGA+GGG | + | chr1.3:60130409-60130428 | MS.gene20988:CDS | 40.0% | |
| ACTATGGTCCTGATGGTTTA+AGG | + | chr1.3:60130478-60130497 | MS.gene20988:CDS | 40.0% | |
| ACTCTTGGTGGAATTGGAAA+TGG | + | chr1.3:60130558-60130577 | MS.gene20988:CDS | 40.0% | |
| ATTGGAAATGGAATTGGAGC+TGG | + | chr1.3:60130570-60130589 | MS.gene20988:CDS | 40.0% | |
| GGTGATGTTGTGCATTATGT+TGG | + | chr1.3:60130317-60130336 | MS.gene20988:CDS | 40.0% | |
| GGTGGAATTGGAAATGGAAT+TGG | + | chr1.3:60130564-60130583 | MS.gene20988:CDS | 40.0% | |
| GTGCAAAACCTTAAACCATC+AGG | - | chr1.3:60130489-60130508 | None:intergenic | 40.0% | |
| TTTGCTGCAGGTGTAAACTA+TGG | + | chr1.3:60130462-60130481 | MS.gene20988:CDS | 40.0% | |
| ! | TGCATTATGTTGGTTGTCCT+TGG | + | chr1.3:60130327-60130346 | MS.gene20988:CDS | 40.0% |
| !! | AGTAGGAAAAGTATTGCCAC+TGG | - | chr1.3:60130529-60130548 | None:intergenic | 40.0% |
| CCTCCCATGTTGAAAGTTTC+AGG | - | chr1.3:60130408-60130427 | None:intergenic | 45.0% | |
| CCTGAAACTTTCAACATGGG+AGG | + | chr1.3:60130405-60130424 | MS.gene20988:CDS | 45.0% | |
| CTTCCACATGAACTGAACCA+AGG | - | chr1.3:60130347-60130366 | None:intergenic | 45.0% | |
| CTTGGTTCAGTTCATGTGGA+AGG | + | chr1.3:60130345-60130364 | MS.gene20988:CDS | 45.0% | |
| GAAACTTTCAACATGGGAGG+AGG | + | chr1.3:60130408-60130427 | MS.gene20988:CDS | 45.0% | |
| GGTGTAAACTATGGTCCTGA+TGG | + | chr1.3:60130471-60130490 | MS.gene20988:CDS | 45.0% | |
| TGTCCTTGGTTCAGTTCATG+TGG | + | chr1.3:60130341-60130360 | MS.gene20988:CDS | 45.0% | |
| ! | TTAAGGTTTTGCACCTTCCC+TGG | + | chr1.3:60130495-60130514 | MS.gene20988:CDS | 45.0% |
| CAATTCCACCAAGAGTCCCA+GGG | - | chr1.3:60130554-60130573 | None:intergenic | 50.0% | |
| TCAACATGGGAGGAGGGAAT+GGG | + | chr1.3:60130415-60130434 | MS.gene20988:CDS | 50.0% | |
| TCAGTTCATGTGGAAGGAGC+TGG | + | chr1.3:60130351-60130370 | MS.gene20988:CDS | 50.0% | |
| TTCAACATGGGAGGAGGGAA+TGG | + | chr1.3:60130414-60130433 | MS.gene20988:CDS | 50.0% | |
| TTCATGTGGAAGGAGCTGGA+AGG | + | chr1.3:60130355-60130374 | MS.gene20988:CDS | 50.0% | |
| ! | GGTGAGAGTTGCAAAGGTGT+TGG | + | chr1.3:60130296-60130315 | MS.gene20988:CDS | 50.0% |
| ACACCTGCAGCAAAGTTGCC+AGG | - | chr1.3:60130456-60130475 | None:intergenic | 55.0% | |
| ACTATCCCTGGGACTCTTGG+TGG | + | chr1.3:60130546-60130565 | MS.gene20988:CDS | 55.0% | |
| AGCTGGAAGGTTGCTGAAGG+AGG | + | chr1.3:60130368-60130387 | MS.gene20988:CDS | 55.0% | |
| AGGAGCTGGAAGGTTGCTGA+AGG | + | chr1.3:60130365-60130384 | MS.gene20988:CDS | 55.0% | |
| CACCTGCAGCAAAGTTGCCA+GGG | - | chr1.3:60130455-60130474 | None:intergenic | 55.0% | |
| CCAAGAGTCCCAGGGATAGT+AGG | - | chr1.3:60130546-60130565 | None:intergenic | 55.0% | |
| CCAATTCCACCAAGAGTCCC+AGG | - | chr1.3:60130555-60130574 | None:intergenic | 55.0% | |
| CCTACTATCCCTGGGACTCT+TGG | + | chr1.3:60130543-60130562 | MS.gene20988:CDS | 55.0% | |
| CCTGGGACTCTTGGTGGAAT+TGG | + | chr1.3:60130552-60130571 | MS.gene20988:CDS | 55.0% | |
| ! | AGGTTTTGCACCTTCCCTGG+TGG | + | chr1.3:60130498-60130517 | MS.gene20988:CDS | 55.0% |
| ACTGGTACAGCCACCAGGGA+AGG | - | chr1.3:60130511-60130530 | None:intergenic | 60.0% | |
| AGCAAAGTTGCCAGGGCTGC+TGG | - | chr1.3:60130448-60130467 | None:intergenic | 60.0% | |
| AGCCCTGGCAACTTTGCTGC+AGG | + | chr1.3:60130450-60130469 | MS.gene20988:CDS | 60.0% | |
| GCAAAGTTGCCAGGGCTGCT+GGG | - | chr1.3:60130447-60130466 | None:intergenic | 60.0% | |
| TGCCACTGGTACAGCCACCA+GGG | - | chr1.3:60130515-60130534 | None:intergenic | 60.0% | |
| TTCCCTGGTGGCTGTACCAG+TGG | + | chr1.3:60130510-60130529 | MS.gene20988:CDS | 60.0% | |
| TTGCCACTGGTACAGCCACC+AGG | - | chr1.3:60130516-60130535 | None:intergenic | 60.0% | |
| GGGATACTTCCCAGCAGCCC+TGG | + | chr1.3:60130435-60130454 | MS.gene20988:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 60130294 | 60130614 | 60130294 | ID=MS.gene20988 |
| chr1.3 | mRNA | 60130294 | 60130614 | 60130294 | ID=MS.gene20988.t1;Parent=MS.gene20988 |
| chr1.3 | exon | 60130294 | 60130614 | 60130294 | ID=MS.gene20988.t1.exon1;Parent=MS.gene20988.t1 |
| chr1.3 | CDS | 60130294 | 60130614 | 60130294 | ID=cds.MS.gene20988.t1;Parent=MS.gene20988.t1 |
| Gene Sequence |
| Protein sequence |
