Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23123.t1 | XP_003626220.1 | 98.4 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 6.70E-59 | 236.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23123.t1 | Q9M9W1 | 88.7 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.5e-56 | 218.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23123.t1 | G7KXJ9 | 98.4 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 4.8e-59 | 236.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene23123 | 0.838523 | 2.73E-57 | -1.69E-46 |
| MS.gene051746 | MS.gene23123 | 0.810946 | 8.95E-51 | -1.69E-46 |
| MS.gene051748 | MS.gene23123 | 0.812133 | 4.94E-51 | -1.69E-46 |
| MS.gene051850 | MS.gene23123 | 0.80586 | 1.09E-49 | -1.69E-46 |
| MS.gene051903 | MS.gene23123 | 0.802052 | 6.74E-49 | -1.69E-46 |
| MS.gene052947 | MS.gene23123 | 0.818946 | 1.50E-52 | -1.69E-46 |
| MS.gene053952 | MS.gene23123 | 0.833988 | 3.89E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene23123 | 0.84782 | 9.08E-60 | -1.69E-46 |
| MS.gene057094 | MS.gene23123 | 0.838028 | 3.67E-57 | -1.69E-46 |
| MS.gene06001 | MS.gene23123 | 0.824777 | 6.72E-54 | -1.69E-46 |
| MS.gene060856 | MS.gene23123 | 0.833097 | 6.49E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene93059 | MS.gene23123 | PPI |
| MS.gene23123 | MS.gene008793 | PPI |
| MS.gene23123 | MS.gene008794 | PPI |
| MS.gene23123 | MS.gene033712 | PPI |
| MS.gene97981 | MS.gene23123 | PPI |
| MS.gene23123 | MS.gene033714 | PPI |
| MS.gene23123 | MS.gene000897 | PPI |
| MS.gene23123 | MS.gene08169 | PPI |
| MS.gene067853 | MS.gene23123 | PPI |
| MS.gene23123 | MS.gene93058 | PPI |
| MS.gene042462 | MS.gene23123 | PPI |
| MS.gene23123 | MS.gene065392 | PPI |
| MS.gene23123 | MS.gene056487 | PPI |
| MS.gene23123 | MS.gene050912 | PPI |
| MS.gene23123 | MS.gene072503 | PPI |
| MS.gene23123 | MS.gene73391 | PPI |
| MS.gene23123 | MS.gene41772 | PPI |
| MS.gene056488 | MS.gene23123 | PPI |
| MS.gene23123 | MS.gene024279 | PPI |
| MS.gene23123 | MS.gene97980 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23123.t1 | MTR_7g112770 | 98.387 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 5.42e-87 | 249 |
| MS.gene23123.t1 | MTR_1g088450 | 94.915 | 118 | 6 | 0 | 7 | 124 | 2 | 119 | 8.92e-69 | 202 |
| MS.gene23123.t1 | MTR_1g083430 | 90.400 | 125 | 11 | 1 | 1 | 124 | 1 | 125 | 3.21e-68 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23123.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene23123.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene23123.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene23123.t1 | AT5G27770 | 87.611 | 113 | 14 | 0 | 1 | 113 | 1 | 113 | 9.50e-53 | 162 |
| MS.gene23123.t1 | AT1G02830 | 71.818 | 110 | 31 | 0 | 6 | 115 | 8 | 117 | 1.06e-44 | 142 |
Find 26 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACCCTTCTTCTTTCCCT+TGG | 0.344547 | 7.2:-4138967 | None:intergenic |
| TCCATGATCTTGTCTTCCAC+TGG | 0.448631 | 7.2:-4139014 | None:intergenic |
| GGTGGAAAAGCTGGTGCTCT+TGG | 0.478615 | 7.2:+4139080 | MS.gene23123:CDS |
| ATCAAGGTCGGTGGAAAAGC+TGG | 0.491281 | 7.2:+4139071 | MS.gene23123:CDS |
| ATGAGTCGTGGTAACGCAGC+TGG | 0.519123 | 7.2:+4138942 | MS.gene23123:CDS |
| CACTGGCTTAGCACAGTCAA+TGG | 0.524581 | 7.2:-4138997 | None:intergenic |
| CATTGCTGAGAACGAGGGAG+AGG | 0.527747 | 7.2:+4140364 | MS.gene23123:CDS |
| TGGTGCCAAGGGAAAGAAGA+AGG | 0.529349 | 7.2:+4138962 | MS.gene23123:CDS |
| CTTGCTCTTCTCACGGGTAA+CGG | 0.534877 | 7.2:-4139114 | None:intergenic |
| TGGTAACGCAGCTGGTGCCA+AGG | 0.535573 | 7.2:+4138950 | MS.gene23123:CDS |
| GTTTCTTCAAGAGCGAATCA+AGG | 0.539769 | 7.2:+4139055 | MS.gene23123:CDS |
| ATAGAAGTATCTATGAGCTC+AGG | 0.546088 | 7.2:+4140333 | MS.gene23123:CDS |
| AGTGATCTTGCTCTTCTCAC+GGG | 0.552636 | 7.2:-4139120 | None:intergenic |
| CAGTGATCTTGCTCTTCTCA+CGG | 0.553223 | 7.2:-4139121 | None:intergenic |
| GCCAGTGGAAGACAAGATCA+TGG | 0.553292 | 7.2:+4139013 | MS.gene23123:CDS |
| CTTCTCACGGGTAACGGTGA+CGG | 0.568235 | 7.2:-4139108 | None:intergenic |
| AGAAACACAATGTAAGAGAC+TGG | 0.568481 | 7.2:+4140285 | MS.gene23123:CDS |
| GGTAACGCAGCTGGTGCCAA+GGG | 0.575575 | 7.2:+4138951 | MS.gene23123:CDS |
| CTTCAACATTGCTGAGAACG+AGG | 0.581013 | 7.2:+4140358 | MS.gene23123:CDS |
| CTTGGAAAAGTTACTGTCAG+AGG | 0.604590 | 7.2:-4139147 | None:intergenic |
| CTTAGCACAGTCAATGGTGA+AGG | 0.610280 | 7.2:-4138991 | None:intergenic |
| CTTCAAGAGCGAATCAAGGT+CGG | 0.612896 | 7.2:+4139059 | MS.gene23123:CDS |
| CATTGACTGTGCTAAGCCAG+TGG | 0.621872 | 7.2:+4138998 | MS.gene23123:CDS |
| CAAGAGCGAATCAAGGTCGG+TGG | 0.643278 | 7.2:+4139062 | MS.gene23123:CDS |
| GGTGCCAAGGGAAAGAAGAA+GGG | 0.657143 | 7.2:+4138963 | MS.gene23123:CDS |
| TTCAACATTGCTGAGAACGA+GGG | 0.691023 | 7.2:+4140359 | MS.gene23123:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGATAAATGATAGTTAT+GGG | - | chr7.2:4139907-4139926 | None:intergenic | 15.0% |
| !!! | GCTTTGATTTAATATTTTAT+TGG | + | chr7.2:4139226-4139245 | MS.gene23123:intron | 15.0% |
| !! | AAAGATAAATGATAGAGTAA+TGG | - | chr7.2:4139852-4139871 | None:intergenic | 20.0% |
| !! | AAGATAAATGATAGAGTAAT+GGG | - | chr7.2:4139851-4139870 | None:intergenic | 20.0% |
| !! | ATACTATATTCTGTAGATAT+AGG | - | chr7.2:4140032-4140051 | None:intergenic | 20.0% |
| !! | ATATCTACAGAATATAGTAT+TGG | + | chr7.2:4140032-4140051 | MS.gene23123:intron | 20.0% |
| !! | GTAAAGATAAATGATAGTTA+TGG | - | chr7.2:4139908-4139927 | None:intergenic | 20.0% |
| !! | TATCTACAGAATATAGTATT+GGG | + | chr7.2:4140033-4140052 | MS.gene23123:intron | 20.0% |
| !!! | ACATCTTATTTATAGCTTAT+TGG | + | chr7.2:4139586-4139605 | MS.gene23123:intron | 20.0% |
| !!! | TAATAAGTGTTCTTTAAGAT+AGG | + | chr7.2:4139505-4139524 | MS.gene23123:intron | 20.0% |
| ! | AATAAGATGTTTATCCAAAC+AGG | - | chr7.2:4139576-4139595 | None:intergenic | 25.0% |
| ! | ATAACTTAGTGCTTATACAA+CGG | + | chr7.2:4139536-4139555 | MS.gene23123:intron | 25.0% |
| ! | ATTCAGTGAAGATAAGTTAT+TGG | + | chr7.2:4139273-4139292 | MS.gene23123:intron | 25.0% |
| !! | TTGCTTGGTTGATTTATTTA+TGG | + | chr7.2:4139313-4139332 | MS.gene23123:intron | 25.0% |
| !!! | AAGATAGTTGATAGTCATTT+TGG | + | chr7.2:4139347-4139366 | MS.gene23123:intron | 25.0% |
| !!! | ATCTGATATTTTGCTGTTTT+TGG | + | chr7.2:4140162-4140181 | MS.gene23123:intron | 25.0% |
| !!! | TCATTTTGGTTTCAATTTGT+TGG | + | chr7.2:4139361-4139380 | MS.gene23123:intron | 25.0% |
| !!! | TCTGATATTTTGCTGTTTTT+GGG | + | chr7.2:4140163-4140182 | MS.gene23123:intron | 25.0% |
| AAGTTATTGGCATGAAATCA+TGG | + | chr7.2:4139286-4139305 | MS.gene23123:intron | 30.0% | |
| AGACCTTATGTTATTAGTTG+CGG | - | chr7.2:4140206-4140225 | None:intergenic | 30.0% | |
| AGTACGATAACAACAGTATA+AGG | + | chr7.2:4140121-4140140 | MS.gene23123:intron | 30.0% | |
| AGTTATGGGACTGTTAAAAT+TGG | - | chr7.2:4139893-4139912 | None:intergenic | 30.0% | |
| CAGTATAAGGTCTACTTAAT+TGG | + | chr7.2:4140134-4140153 | MS.gene23123:intron | 30.0% | |
| GTAAATGAATTATTACCGCT+TGG | - | chr7.2:4139168-4139187 | None:intergenic | 30.0% | |
| TATACAACGGCAATATAGAT+AGG | + | chr7.2:4139549-4139568 | MS.gene23123:intron | 30.0% | |
| TCAAGTAGTCTCACAATTTA+TGG | + | chr7.2:4139790-4139809 | MS.gene23123:intron | 30.0% | |
| TCAGCAAACTAATTCATTGA+AGG | + | chr7.2:4140059-4140078 | MS.gene23123:intron | 30.0% | |
| TTTCCGCAACTAATAACATA+AGG | + | chr7.2:4140200-4140219 | MS.gene23123:intron | 30.0% | |
| ! | ATAAGTTTTGCGAGACTATT+TGG | + | chr7.2:4139421-4139440 | MS.gene23123:intron | 30.0% |
| ! | TAAGTTTTGCGAGACTATTT+GGG | + | chr7.2:4139422-4139441 | MS.gene23123:intron | 30.0% |
| !! | GTGTTCTTTAAGATAGGTTA+TGG | + | chr7.2:4139511-4139530 | MS.gene23123:intron | 30.0% |
| !! | TTTCAATTTGTTGGTCTGTT+TGG | + | chr7.2:4139370-4139389 | MS.gene23123:intron | 30.0% |
| AACTTACGAACAAGAGCTTA+TGG | + | chr7.2:4139447-4139466 | MS.gene23123:intron | 35.0% | |
| AGAAACACAATGTAAGAGAC+TGG | + | chr7.2:4140285-4140304 | MS.gene23123:CDS | 35.0% | |
| AGTAGTCTCACAATTTATGG+CGG | + | chr7.2:4139793-4139812 | MS.gene23123:intron | 35.0% | |
| CAATATAGATAGGTCCTGTT+TGG | + | chr7.2:4139559-4139578 | MS.gene23123:intron | 35.0% | |
| GCTGTCTGAATAAGCTATAT+TGG | + | chr7.2:4139712-4139731 | MS.gene23123:intron | 35.0% | |
| GCTTATTTGAGCTTGTCTAT+GGG | + | chr7.2:4139397-4139416 | MS.gene23123:intron | 35.0% | |
| TGAAATCATGGTTCATTGCT+TGG | + | chr7.2:4139298-4139317 | MS.gene23123:intron | 35.0% | |
| ! | AGTAATGGGACTGTTAAGAT+TGG | - | chr7.2:4139837-4139856 | None:intergenic | 35.0% |
| ! | ATTTGTTGGTCTGTTTGGAT+TGG | + | chr7.2:4139375-4139394 | MS.gene23123:intron | 35.0% |
| ! | CAAATCATTGTTTGCTACTC+TGG | + | chr7.2:4139187-4139206 | MS.gene23123:intron | 35.0% |
| !! | ATAGAAGTATCTATGAGCTC+AGG | + | chr7.2:4140333-4140352 | MS.gene23123:CDS | 35.0% |
| AATTGGCACTACGTATAAGC+TGG | - | chr7.2:4139876-4139895 | None:intergenic | 40.0% | |
| ATAAGCTGTCTATCCCAACA+CGG | + | chr7.2:4140006-4140025 | MS.gene23123:intron | 40.0% | |
| CTTGGAAAAGTTACTGTCAG+AGG | - | chr7.2:4139150-4139169 | None:intergenic | 40.0% | |
| GGCTTATTTGAGCTTGTCTA+TGG | + | chr7.2:4139396-4139415 | MS.gene23123:intron | 40.0% | |
| GTTTCTTCAAGAGCGAATCA+AGG | + | chr7.2:4139055-4139074 | MS.gene23123:CDS | 40.0% | |
| TTCAACATTGCTGAGAACGA+GGG | + | chr7.2:4140359-4140378 | MS.gene23123:CDS | 40.0% | |
| ! | CTGACAGTAACTTTTCCAAG+CGG | + | chr7.2:4139150-4139169 | MS.gene23123:CDS | 40.0% |
| AGTGATCTTGCTCTTCTCAC+GGG | - | chr7.2:4139123-4139142 | None:intergenic | 45.0% | |
| CAGTGATCTTGCTCTTCTCA+CGG | - | chr7.2:4139124-4139143 | None:intergenic | 45.0% | |
| CTGTAGATATAGGCCGTGTT+GGG | - | chr7.2:4140022-4140041 | None:intergenic | 45.0% | |
| CTTAGCACAGTCAATGGTGA+AGG | - | chr7.2:4138994-4139013 | None:intergenic | 45.0% | |
| CTTCAACATTGCTGAGAACG+AGG | + | chr7.2:4140358-4140377 | MS.gene23123:CDS | 45.0% | |
| CTTCAAGAGCGAATCAAGGT+CGG | + | chr7.2:4139059-4139078 | MS.gene23123:CDS | 45.0% | |
| TCCATGATCTTGTCTTCCAC+TGG | - | chr7.2:4139017-4139036 | None:intergenic | 45.0% | |
| TCTGTAGATATAGGCCGTGT+TGG | - | chr7.2:4140023-4140042 | None:intergenic | 45.0% | |
| ATCAAGGTCGGTGGAAAAGC+TGG | + | chr7.2:4139071-4139090 | MS.gene23123:CDS | 50.0% | |
| CTTGCTCTTCTCACGGGTAA+CGG | - | chr7.2:4139117-4139136 | None:intergenic | 50.0% | |
| GCCAGTGGAAGACAAGATCA+TGG | + | chr7.2:4139013-4139032 | MS.gene23123:CDS | 50.0% | |
| GGTGCCAAGGGAAAGAAGAA+GGG | + | chr7.2:4138963-4138982 | MS.gene23123:CDS | 50.0% | |
| TGCACCCTTCTTCTTTCCCT+TGG | - | chr7.2:4138970-4138989 | None:intergenic | 50.0% | |
| ! | CATTGACTGTGCTAAGCCAG+TGG | + | chr7.2:4138998-4139017 | MS.gene23123:CDS | 50.0% |
| ! | TGGTGCCAAGGGAAAGAAGA+AGG | + | chr7.2:4138962-4138981 | MS.gene23123:CDS | 50.0% |
| !! | CACTGGCTTAGCACAGTCAA+TGG | - | chr7.2:4139000-4139019 | None:intergenic | 50.0% |
| CAAGAGCGAATCAAGGTCGG+TGG | + | chr7.2:4139062-4139081 | MS.gene23123:CDS | 55.0% | |
| CATTGCTGAGAACGAGGGAG+AGG | + | chr7.2:4140364-4140383 | MS.gene23123:CDS | 55.0% | |
| GGTGGAAAAGCTGGTGCTCT+TGG | + | chr7.2:4139080-4139099 | MS.gene23123:CDS | 55.0% | |
| ! | ATGAGTCGTGGTAACGCAGC+TGG | + | chr7.2:4138942-4138961 | MS.gene23123:CDS | 55.0% |
| ! | CTTCTCACGGGTAACGGTGA+CGG | - | chr7.2:4139111-4139130 | None:intergenic | 55.0% |
| ! | GGTAACGCAGCTGGTGCCAA+GGG | + | chr7.2:4138951-4138970 | MS.gene23123:CDS | 60.0% |
| ! | TGGTAACGCAGCTGGTGCCA+AGG | + | chr7.2:4138950-4138969 | MS.gene23123:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 4138942 | 4140397 | 4138942 | ID=MS.gene23123 |
| chr7.2 | mRNA | 4138942 | 4140397 | 4138942 | ID=MS.gene23123.t1;Parent=MS.gene23123 |
| chr7.2 | exon | 4138942 | 4139171 | 4138942 | ID=MS.gene23123.t1.exon1;Parent=MS.gene23123.t1 |
| chr7.2 | CDS | 4138942 | 4139171 | 4138942 | ID=cds.MS.gene23123.t1;Parent=MS.gene23123.t1 |
| chr7.2 | exon | 4140253 | 4140397 | 4140253 | ID=MS.gene23123.t1.exon2;Parent=MS.gene23123.t1 |
| chr7.2 | CDS | 4140253 | 4140397 | 4140253 | ID=cds.MS.gene23123.t1;Parent=MS.gene23123.t1 |
| Gene Sequence |
| Protein sequence |
