Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072503.t1 | XP_003626220.1 | 98.4 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 6.70E-59 | 236.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072503.t1 | Q9M9W1 | 88.7 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.5e-56 | 218.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072503.t1 | G7KXJ9 | 98.4 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 4.8e-59 | 236.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene072503 | 0.805084 | 1.58E-49 | -1.69E-46 |
| MS.gene050134 | MS.gene072503 | 0.865238 | 6.83E-65 | -1.69E-46 |
| MS.gene050791 | MS.gene072503 | 0.890024 | 1.43E-73 | -1.69E-46 |
| MS.gene050912 | MS.gene072503 | 0.970308 | 2.03E-131 | -1.69E-46 |
| MS.gene051984 | MS.gene072503 | 0.821774 | 3.38E-53 | -1.69E-46 |
| MS.gene052664 | MS.gene072503 | 0.81968 | 1.02E-52 | -1.69E-46 |
| MS.gene053224 | MS.gene072503 | 0.854015 | 1.63E-61 | -1.69E-46 |
| MS.gene057272 | MS.gene072503 | 0.878374 | 2.96E-69 | -1.69E-46 |
| MS.gene057641 | MS.gene072503 | 0.84724 | 1.31E-59 | -1.69E-46 |
| MS.gene057643 | MS.gene072503 | 0.851257 | 9.99E-61 | -1.69E-46 |
| MS.gene060483 | MS.gene072503 | 0.802442 | 5.60E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072503 | MS.gene065392 | PPI |
| MS.gene072503 | MS.gene024279 | PPI |
| MS.gene072503 | MS.gene97980 | PPI |
| MS.gene93059 | MS.gene072503 | PPI |
| MS.gene072503 | MS.gene41772 | PPI |
| MS.gene072503 | MS.gene008793 | PPI |
| MS.gene97981 | MS.gene072503 | PPI |
| MS.gene072503 | MS.gene000897 | PPI |
| MS.gene056487 | MS.gene072503 | PPI |
| MS.gene067853 | MS.gene072503 | PPI |
| MS.gene042462 | MS.gene072503 | PPI |
| MS.gene033714 | MS.gene072503 | PPI |
| MS.gene072503 | MS.gene008794 | PPI |
| MS.gene23123 | MS.gene072503 | PPI |
| MS.gene033712 | MS.gene072503 | PPI |
| MS.gene072503 | MS.gene93058 | PPI |
| MS.gene050912 | MS.gene072503 | PPI |
| MS.gene072503 | MS.gene73391 | PPI |
| MS.gene056488 | MS.gene072503 | PPI |
| MS.gene08169 | MS.gene072503 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072503.t1 | MTR_7g112770 | 98.387 | 124 | 2 | 0 | 1 | 124 | 1 | 124 | 5.42e-87 | 249 |
| MS.gene072503.t1 | MTR_1g088450 | 94.915 | 118 | 6 | 0 | 7 | 124 | 2 | 119 | 8.92e-69 | 202 |
| MS.gene072503.t1 | MTR_1g083430 | 90.400 | 125 | 11 | 1 | 1 | 124 | 1 | 125 | 3.21e-68 | 201 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072503.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene072503.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene072503.t1 | AT3G05560 | 88.710 | 124 | 14 | 0 | 1 | 124 | 1 | 124 | 3.08e-68 | 201 |
| MS.gene072503.t1 | AT5G27770 | 87.611 | 113 | 14 | 0 | 1 | 113 | 1 | 113 | 9.50e-53 | 162 |
| MS.gene072503.t1 | AT1G02830 | 71.818 | 110 | 31 | 0 | 6 | 115 | 8 | 117 | 1.06e-44 | 142 |
Find 26 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCACCCTTCTTCTTTCCCT+TGG | 0.344547 | 7.3:-3902657 | None:intergenic |
| TCCATGATCTTGTCTTCCAC+TGG | 0.448631 | 7.3:-3902704 | None:intergenic |
| GGTGGAAAAGCTGGTGCTCT+TGG | 0.478615 | 7.3:+3902770 | MS.gene072503:CDS |
| ATCAAGGTCGGTGGAAAAGC+TGG | 0.491281 | 7.3:+3902761 | MS.gene072503:CDS |
| ATGAGTCGTGGTAACGCAGC+TGG | 0.519123 | 7.3:+3902632 | MS.gene072503:CDS |
| CACTGGCTTAGCACAGTCAA+TGG | 0.524581 | 7.3:-3902687 | None:intergenic |
| CATTGCTGAGAACGAGGGAG+AGG | 0.527747 | 7.3:+3904054 | MS.gene072503:CDS |
| TGGTGCCAAGGGAAAGAAGA+AGG | 0.529349 | 7.3:+3902652 | MS.gene072503:CDS |
| CTTGCTCTTCTCACGGGTAA+CGG | 0.534877 | 7.3:-3902804 | None:intergenic |
| TGGTAACGCAGCTGGTGCCA+AGG | 0.535573 | 7.3:+3902640 | MS.gene072503:CDS |
| GTTTCTTCAAGAGCGAATCA+AGG | 0.539769 | 7.3:+3902745 | MS.gene072503:CDS |
| ATAGAAGTATCTATGAGCTC+AGG | 0.546088 | 7.3:+3904023 | MS.gene072503:CDS |
| AGTGATCTTGCTCTTCTCAC+GGG | 0.552636 | 7.3:-3902810 | None:intergenic |
| CAGTGATCTTGCTCTTCTCA+CGG | 0.553223 | 7.3:-3902811 | None:intergenic |
| GCCAGTGGAAGACAAGATCA+TGG | 0.553292 | 7.3:+3902703 | MS.gene072503:CDS |
| CTTCTCACGGGTAACGGTGA+CGG | 0.568235 | 7.3:-3902798 | None:intergenic |
| AGAAACACAATGTAAGAGAC+TGG | 0.568481 | 7.3:+3903975 | MS.gene072503:CDS |
| GGTAACGCAGCTGGTGCCAA+GGG | 0.575575 | 7.3:+3902641 | MS.gene072503:CDS |
| CTTCAACATTGCTGAGAACG+AGG | 0.581013 | 7.3:+3904048 | MS.gene072503:CDS |
| CTTGGAAAAGTTACTGTCAG+AGG | 0.604590 | 7.3:-3902837 | None:intergenic |
| CTTAGCACAGTCAATGGTGA+AGG | 0.610280 | 7.3:-3902681 | None:intergenic |
| CTTCAAGAGCGAATCAAGGT+CGG | 0.612896 | 7.3:+3902749 | MS.gene072503:CDS |
| CATTGACTGTGCTAAGCCAG+TGG | 0.621872 | 7.3:+3902688 | MS.gene072503:CDS |
| CAAGAGCGAATCAAGGTCGG+TGG | 0.643278 | 7.3:+3902752 | MS.gene072503:CDS |
| GGTGCCAAGGGAAAGAAGAA+GGG | 0.657143 | 7.3:+3902653 | MS.gene072503:CDS |
| TTCAACATTGCTGAGAACGA+GGG | 0.691023 | 7.3:+3904049 | MS.gene072503:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAGATAAATGATAGTTAT+GGG | - | chr7.3:3903597-3903616 | None:intergenic | 15.0% |
| !!! | GCTTTGATTTAATATTTTAT+TGG | + | chr7.3:3902916-3902935 | MS.gene072503:intron | 15.0% |
| !! | AAAGATAAATGATAGAGTAA+TGG | - | chr7.3:3903542-3903561 | None:intergenic | 20.0% |
| !! | AAGATAAATGATAGAGTAAT+GGG | - | chr7.3:3903541-3903560 | None:intergenic | 20.0% |
| !! | ACATCTAATTAATAGCTTAT+TGG | + | chr7.3:3903272-3903291 | MS.gene072503:intron | 20.0% |
| !! | ATACTATATTCTGTAGATAT+AGG | - | chr7.3:3903722-3903741 | None:intergenic | 20.0% |
| !! | ATATCTACAGAATATAGTAT+TGG | + | chr7.3:3903722-3903741 | MS.gene072503:intron | 20.0% |
| !! | GTAAAGATAAATGATAGTTA+TGG | - | chr7.3:3903598-3903617 | None:intergenic | 20.0% |
| !! | TATCTACAGAATATAGTATT+GGG | + | chr7.3:3903723-3903742 | MS.gene072503:intron | 20.0% |
| !!! | TAATAAGTGTTCTTTAAGAT+AGG | + | chr7.3:3903195-3903214 | MS.gene072503:intron | 20.0% |
| ! | AAGTAGTCTCACAAAATTTA+TGG | + | chr7.3:3903480-3903499 | MS.gene072503:intron | 25.0% |
| ! | AATTAGATGTTTATCCAAAC+AGG | - | chr7.3:3903262-3903281 | None:intergenic | 25.0% |
| ! | ATAACTTAGTGCTTATACAA+CGG | + | chr7.3:3903226-3903245 | MS.gene072503:intron | 25.0% |
| ! | ATTCAGTGAAGATAAGTTAT+TGG | + | chr7.3:3902963-3902982 | MS.gene072503:intron | 25.0% |
| !! | ATAAGTTTTGCGAAACTATT+TGG | + | chr7.3:3903111-3903130 | MS.gene072503:intron | 25.0% |
| !! | TAAGTTTTGCGAAACTATTT+GGG | + | chr7.3:3903112-3903131 | MS.gene072503:intron | 25.0% |
| !! | TTGCTTGGTTGATTTATTTA+TGG | + | chr7.3:3903003-3903022 | MS.gene072503:intron | 25.0% |
| !!! | AAGATAGTTGATAGTCATTT+TGG | + | chr7.3:3903037-3903056 | MS.gene072503:intron | 25.0% |
| !!! | TCATTTTGGTTTCAATTTGT+TGG | + | chr7.3:3903051-3903070 | MS.gene072503:intron | 25.0% |
| AAGTTATTGGCATGAAATCA+TGG | + | chr7.3:3902976-3902995 | MS.gene072503:intron | 30.0% | |
| AGACCTTATGTTATTAGTTG+CGG | - | chr7.3:3903896-3903915 | None:intergenic | 30.0% | |
| AGTACGATAACAACAGTATA+AGG | + | chr7.3:3903811-3903830 | MS.gene072503:intron | 30.0% | |
| AGTTATGGGACTGTTAAAAT+TGG | - | chr7.3:3903583-3903602 | None:intergenic | 30.0% | |
| CAGTATAAGGTCTACTTAAT+TGG | + | chr7.3:3903824-3903843 | MS.gene072503:intron | 30.0% | |
| GTAAATGAATTATTACCGCT+TGG | - | chr7.3:3902858-3902877 | None:intergenic | 30.0% | |
| TAGTCTCACAAAATTTATGG+CGG | + | chr7.3:3903483-3903502 | MS.gene072503:intron | 30.0% | |
| TCAGCAAACTAATTCATTGA+AGG | + | chr7.3:3903749-3903768 | MS.gene072503:intron | 30.0% | |
| TTTCCGCAACTAATAACATA+AGG | + | chr7.3:3903890-3903909 | MS.gene072503:intron | 30.0% | |
| !! | GTGTTCTTTAAGATAGGTTA+TGG | + | chr7.3:3903201-3903220 | MS.gene072503:intron | 30.0% |
| !! | TTTCAATTTGTTGGTCTGTT+TGG | + | chr7.3:3903060-3903079 | MS.gene072503:intron | 30.0% |
| !!! | AGCTGATATTTTGCTGTTTT+TGG | + | chr7.3:3903852-3903871 | MS.gene072503:intron | 30.0% |
| !!! | GCTGATATTTTGCTGTTTTT+GGG | + | chr7.3:3903853-3903872 | MS.gene072503:intron | 30.0% |
| AACTTACGAACAAGAGCTTA+TGG | + | chr7.3:3903137-3903156 | MS.gene072503:intron | 35.0% | |
| AGAAACACAATGTAAGAGAC+TGG | + | chr7.3:3903975-3903994 | MS.gene072503:CDS | 35.0% | |
| GCTGTCTGAATAAGCTATAT+TGG | + | chr7.3:3903400-3903419 | MS.gene072503:intron | 35.0% | |
| GCTTATTTGAGCTTGTCTAT+GGG | + | chr7.3:3903087-3903106 | MS.gene072503:intron | 35.0% | |
| TGAAATCATGGTTCATTGCT+TGG | + | chr7.3:3902988-3903007 | MS.gene072503:intron | 35.0% | |
| TGCTTATACAACGGCAATAT+AGG | + | chr7.3:3903235-3903254 | MS.gene072503:intron | 35.0% | |
| ! | AGTAATGGGACTGTTAAGAT+TGG | - | chr7.3:3903527-3903546 | None:intergenic | 35.0% |
| ! | ATTTGTTGGTCTGTTTGGAT+TGG | + | chr7.3:3903065-3903084 | MS.gene072503:intron | 35.0% |
| ! | CAAATCATTGTTTGCTACTC+TGG | + | chr7.3:3902877-3902896 | MS.gene072503:intron | 35.0% |
| !! | ATAGAAGTATCTATGAGCTC+AGG | + | chr7.3:3904023-3904042 | MS.gene072503:CDS | 35.0% |
| ATAAGCTGTCTATCCCAACA+CGG | + | chr7.3:3903696-3903715 | MS.gene072503:intron | 40.0% | |
| CTTGGAAAAGTTACTGTCAG+AGG | - | chr7.3:3902840-3902859 | None:intergenic | 40.0% | |
| GGCTTATTTGAGCTTGTCTA+TGG | + | chr7.3:3903086-3903105 | MS.gene072503:intron | 40.0% | |
| GTTTCTTCAAGAGCGAATCA+AGG | + | chr7.3:3902745-3902764 | MS.gene072503:CDS | 40.0% | |
| TTCAACATTGCTGAGAACGA+GGG | + | chr7.3:3904049-3904068 | MS.gene072503:CDS | 40.0% | |
| ! | CTGACAGTAACTTTTCCAAG+CGG | + | chr7.3:3902840-3902859 | MS.gene072503:CDS | 40.0% |
| ACGGCAATATAGGTCCTGTT+TGG | + | chr7.3:3903245-3903264 | MS.gene072503:intron | 45.0% | |
| AGTGATCTTGCTCTTCTCAC+GGG | - | chr7.3:3902813-3902832 | None:intergenic | 45.0% | |
| CAGTGATCTTGCTCTTCTCA+CGG | - | chr7.3:3902814-3902833 | None:intergenic | 45.0% | |
| CTGTAGATATAGGCCGTGTT+GGG | - | chr7.3:3903712-3903731 | None:intergenic | 45.0% | |
| CTTAGCACAGTCAATGGTGA+AGG | - | chr7.3:3902684-3902703 | None:intergenic | 45.0% | |
| CTTCAACATTGCTGAGAACG+AGG | + | chr7.3:3904048-3904067 | MS.gene072503:CDS | 45.0% | |
| CTTCAAGAGCGAATCAAGGT+CGG | + | chr7.3:3902749-3902768 | MS.gene072503:CDS | 45.0% | |
| TCCATGATCTTGTCTTCCAC+TGG | - | chr7.3:3902707-3902726 | None:intergenic | 45.0% | |
| TCTGTAGATATAGGCCGTGT+TGG | - | chr7.3:3903713-3903732 | None:intergenic | 45.0% | |
| ATCAAGGTCGGTGGAAAAGC+TGG | + | chr7.3:3902761-3902780 | MS.gene072503:CDS | 50.0% | |
| CTTGCTCTTCTCACGGGTAA+CGG | - | chr7.3:3902807-3902826 | None:intergenic | 50.0% | |
| GCCAGTGGAAGACAAGATCA+TGG | + | chr7.3:3902703-3902722 | MS.gene072503:CDS | 50.0% | |
| GGTGCCAAGGGAAAGAAGAA+GGG | + | chr7.3:3902653-3902672 | MS.gene072503:CDS | 50.0% | |
| TGCACCCTTCTTCTTTCCCT+TGG | - | chr7.3:3902660-3902679 | None:intergenic | 50.0% | |
| ! | CATTGACTGTGCTAAGCCAG+TGG | + | chr7.3:3902688-3902707 | MS.gene072503:CDS | 50.0% |
| ! | TGGTGCCAAGGGAAAGAAGA+AGG | + | chr7.3:3902652-3902671 | MS.gene072503:CDS | 50.0% |
| !! | CACTGGCTTAGCACAGTCAA+TGG | - | chr7.3:3902690-3902709 | None:intergenic | 50.0% |
| CAAGAGCGAATCAAGGTCGG+TGG | + | chr7.3:3902752-3902771 | MS.gene072503:CDS | 55.0% | |
| CATTGCTGAGAACGAGGGAG+AGG | + | chr7.3:3904054-3904073 | MS.gene072503:CDS | 55.0% | |
| GGTGGAAAAGCTGGTGCTCT+TGG | + | chr7.3:3902770-3902789 | MS.gene072503:CDS | 55.0% | |
| ! | ATGAGTCGTGGTAACGCAGC+TGG | + | chr7.3:3902632-3902651 | MS.gene072503:CDS | 55.0% |
| ! | CTTCTCACGGGTAACGGTGA+CGG | - | chr7.3:3902801-3902820 | None:intergenic | 55.0% |
| ! | GGTAACGCAGCTGGTGCCAA+GGG | + | chr7.3:3902641-3902660 | MS.gene072503:CDS | 60.0% |
| ! | TGGTAACGCAGCTGGTGCCA+AGG | + | chr7.3:3902640-3902659 | MS.gene072503:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 3902632 | 3904087 | 3902632 | ID=MS.gene072503 |
| chr7.3 | mRNA | 3902632 | 3904087 | 3902632 | ID=MS.gene072503.t1;Parent=MS.gene072503 |
| chr7.3 | exon | 3902632 | 3902861 | 3902632 | ID=MS.gene072503.t1.exon1;Parent=MS.gene072503.t1 |
| chr7.3 | CDS | 3902632 | 3902861 | 3902632 | ID=cds.MS.gene072503.t1;Parent=MS.gene072503.t1 |
| chr7.3 | exon | 3903943 | 3904087 | 3903943 | ID=MS.gene072503.t1.exon2;Parent=MS.gene072503.t1 |
| chr7.3 | CDS | 3903943 | 3904087 | 3903943 | ID=cds.MS.gene072503.t1;Parent=MS.gene072503.t1 |
| Gene Sequence |
| Protein sequence |
