AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27301


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27301.t1 MTR_1g022355 95.357 280 10 1 1 280 90 366 0.0 535
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27301.t1 AT1G19520 56.631 279 100 5 1 274 108 370 2.72e-87 265
MS.gene27301.t1 AT1G19520 56.429 280 101 5 1 275 108 371 2.37e-84 267

Find 74 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGTTCAAGATTTA+TGG 0.133676 1.2:-2222537 MS.gene27301:CDS
TCAGCCACTTGTTCCCATTT+AGG 0.207802 1.2:+2222498 None:intergenic
TTGATCTATCGGATCCTAAA+TGG 0.216797 1.2:-2222512 MS.gene27301:CDS
ATGAGGCGGGAGAAGTTATT+TGG 0.238365 1.2:-2222467 MS.gene27301:CDS
TTAGAGAGCTTAGGTTTAAA+TGG 0.238681 1.2:-2222743 MS.gene27301:CDS
GGAGGTTAGTAATAAGGTTT+TGG 0.281718 1.2:-2222877 MS.gene27301:CDS
TGTTTGCATTTCCCGGTTAT+CGG 0.314823 1.2:+2222429 None:intergenic
GGTAACTTGTTTGCATTTCC+CGG 0.333463 1.2:+2222422 None:intergenic
GAATTATCCTTTATACTTCA+AGG 0.334354 1.2:-2222271 MS.gene27301:CDS
AAGGAGGCGAAGCCGATAAC+CGG 0.340946 1.2:-2222441 MS.gene27301:CDS
TGTGTGGGAGGTTAGTAATA+AGG 0.359950 1.2:-2222883 MS.gene27301:CDS
TAGAGCTTCTACAGCCTACT+AGG 0.360591 1.2:-2222332 MS.gene27301:CDS
TGAAAGGGTTGTTTCAGAAA+TGG 0.373951 1.2:-2222994 MS.gene27301:CDS
TAAATGGGCACGTGAGAAAA+TGG 0.380143 1.2:-2222727 MS.gene27301:CDS
TGCATCTTCGCGATTTGATT+CGG 0.380839 1.2:-2222934 MS.gene27301:CDS
ATGGAAAAGGGTAGTGGTAG+TGG 0.392895 1.2:-2222975 MS.gene27301:CDS
ACAGTTACCTTGAAGTATAA+AGG 0.399658 1.2:+2222264 None:intergenic
TGTAGATTTGCTGGTGAAGT+TGG 0.408581 1.2:-2222783 MS.gene27301:CDS
GATGCTGGTGTTCGTGTTGA+TGG 0.410669 1.2:-2222606 MS.gene27301:CDS
AAATTAAGTTCAAGATTTAT+GGG 0.422169 1.2:-2222536 MS.gene27301:CDS
CGAGAAAATTCTGTCGTTGA+AGG 0.433246 1.2:-2222400 MS.gene27301:CDS
AAAGAGATGTGTAGATTTGC+TGG 0.447104 1.2:-2222792 MS.gene27301:CDS
GGTTGTTTCAGAAATGGAAA+AGG 0.448209 1.2:-2222988 MS.gene27301:CDS
GTTGACCCTGGTAGCTGAAT+GGG 0.449689 1.2:-2222355 MS.gene27301:CDS
ATGAGGATTTGGAGAAAATG+AGG 0.450985 1.2:-2222689 MS.gene27301:CDS
TAGAGAGCTTAGGTTTAAAT+GGG 0.451572 1.2:-2222742 MS.gene27301:CDS
TGATCTATCGGATCCTAAAT+GGG 0.452985 1.2:-2222511 MS.gene27301:CDS
GATTTATGGGCTTGATCTAT+CGG 0.456068 1.2:-2222523 MS.gene27301:CDS
TGGAAAAGGGTAGTGGTAGT+GGG 0.463563 1.2:-2222974 MS.gene27301:CDS
GTTGAGGAGAAGAAGGTTGT+TGG 0.471339 1.2:-2222579 MS.gene27301:CDS
TTGATCCAATCCACCCTAGT+AGG 0.474961 1.2:+2222318 None:intergenic
GCTCTACCCATTCAGCTACC+AGG 0.475118 1.2:+2222349 None:intergenic
TTGGTCTTCCTAAGAGGAAA+GGG 0.483400 1.2:-2222560 MS.gene27301:CDS
GCTGGTGAAGTTGGGATTCG+AGG 0.483854 1.2:-2222774 MS.gene27301:CDS
GTTGTTTCAGAAATGGAAAA+GGG 0.487089 1.2:-2222987 MS.gene27301:CDS
TGCTGCTTGTTATTGTTGAA+AGG 0.487258 1.2:-2223010 MS.gene27301:CDS
GAAAATGTTGTTGTTGATGC+TGG 0.487848 1.2:-2222621 MS.gene27301:CDS
AGGAAGAAGGAGAAAATGAA+AGG 0.488535 1.2:-2222837 MS.gene27301:CDS
AACAAGCAGCATTGCATCTA+CGG 0.491489 1.2:+2223022 None:intergenic
ATTGGATCAATTTGCTTGAA+AGG 0.495330 1.2:-2222305 MS.gene27301:CDS
TACAGCCTACTAGGGTGGAT+TGG 0.497105 1.2:-2222323 MS.gene27301:CDS
GTTATCGGCTTCGCCTCCTT+CGG 0.498408 1.2:+2222444 None:intergenic
GTAGATTTGCTGGTGAAGTT+GGG 0.499570 1.2:-2222782 MS.gene27301:CDS
GCGATTTGCTTAGAGAGCTT+AGG 0.500284 1.2:-2222752 MS.gene27301:CDS
TGTTGACCCTGGTAGCTGAA+TGG 0.506521 1.2:-2222356 MS.gene27301:CDS
GCTGCTTGTTATTGTTGAAA+GGG 0.513685 1.2:-2223009 MS.gene27301:CDS
GGAAGAAGGAGAAAATGAAA+GGG 0.516139 1.2:-2222836 MS.gene27301:CDS
TATGAATAAAGAGAGGAAGA+AGG 0.517752 1.2:-2222850 MS.gene27301:CDS
GGAAAAGGGTAGTGGTAGTG+GGG 0.518104 1.2:-2222973 MS.gene27301:CDS
AGGTTGTTGGTCTTCCTAAG+AGG 0.522381 1.2:-2222566 MS.gene27301:CDS
GAAAAGGGTAGTGGTAGTGG+GGG 0.524024 1.2:-2222972 MS.gene27301:CDS
TGGTAATGTTGAGGAGAAGA+AGG 0.529708 1.2:-2222586 MS.gene27301:CDS
AATTCTGTCGTTGAAGGAGA+AGG 0.546483 1.2:-2222394 MS.gene27301:CDS
GTTGGTCTTCCTAAGAGGAA+AGG 0.559694 1.2:-2222561 MS.gene27301:CDS
TCAGAAATGGAAAAGGGTAG+TGG 0.562055 1.2:-2222981 MS.gene27301:CDS
AGGAGGCGAAGCCGATAACC+GGG 0.567405 1.2:-2222440 MS.gene27301:CDS
AGAAGTTATTTGGCCGAAGG+AGG 0.576981 1.2:-2222457 MS.gene27301:CDS
GGCTGATAAGATTCATGAGG+CGG 0.580784 1.2:-2222481 MS.gene27301:CDS
GGGAGAAGTTATTTGGCCGA+AGG 0.586632 1.2:-2222460 MS.gene27301:CDS
GCTGATAAGATTCATGAGGC+GGG 0.589093 1.2:-2222480 MS.gene27301:CDS
TTTATGGAGGACTGTGTGGG+AGG 0.601402 1.2:-2222895 MS.gene27301:CDS
ATCTTCGCGATTTGATTCGG+TGG 0.603612 1.2:-2222931 MS.gene27301:CDS
AGGATTTATGGAGGACTGTG+TGG 0.605077 1.2:-2222899 MS.gene27301:CDS
CTGTCGTTGAAGGAGAAGGA+TGG 0.608593 1.2:-2222390 MS.gene27301:CDS
GGATCCTAAATGGGAACAAG+TGG 0.618929 1.2:-2222502 MS.gene27301:CDS
CTCTACCCATTCAGCTACCA+GGG 0.620249 1.2:+2222350 None:intergenic
GCTTCTACAGCCTACTAGGG+TGG 0.622270 1.2:-2222328 MS.gene27301:CDS
TCGTGTTGATGGTAATGTTG+AGG 0.627189 1.2:-2222595 MS.gene27301:CDS
AGAGCTTCTACAGCCTACTA+GGG 0.634855 1.2:-2222331 MS.gene27301:CDS
GGATTTATGGAGGACTGTGT+GGG 0.636168 1.2:-2222898 MS.gene27301:CDS
TGGTTGATATGAATAAAGAG+AGG 0.637788 1.2:-2222857 MS.gene27301:CDS
AGATGATAGTTTGTTGACCC+TGG 0.658807 1.2:-2222367 MS.gene27301:CDS
AGTGGCTGATAAGATTCATG+AGG 0.693084 1.2:-2222484 MS.gene27301:CDS
ATGGGCACGTGAGAAAATGG+AGG 0.695770 1.2:-2222724 MS.gene27301:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAGTTCAAGATTTA+TGG - chr1.2:2222779-2222798 MS.gene27301:CDS 15.0%
!! AAATTAAGTTCAAGATTTAT+GGG - chr1.2:2222780-2222799 MS.gene27301:CDS 15.0%
! GAATTATCCTTTATACTTCA+AGG - chr1.2:2223045-2223064 MS.gene27301:CDS 25.0%
!! ATCAACAACAACATTTTCTT+CGG + chr1.2:2222693-2222712 None:intergenic 25.0%
ACAGTTACCTTGAAGTATAA+AGG + chr1.2:2223055-2223074 None:intergenic 30.0%
TAGAGAGCTTAGGTTTAAAT+GGG - chr1.2:2222574-2222593 MS.gene27301:CDS 30.0%
TATGAATAAAGAGAGGAAGA+AGG - chr1.2:2222466-2222485 MS.gene27301:CDS 30.0%
TTAGAGAGCTTAGGTTTAAA+TGG - chr1.2:2222573-2222592 MS.gene27301:CDS 30.0%
! ATTGGATCAATTTGCTTGAA+AGG - chr1.2:2223011-2223030 MS.gene27301:CDS 30.0%
! GTTGTTTCAGAAATGGAAAA+GGG - chr1.2:2222329-2222348 MS.gene27301:CDS 30.0%
! TACGGTACTTTTATCACATT+CGG + chr1.2:2222279-2222298 None:intergenic 30.0%
! TGGTTGATATGAATAAAGAG+AGG - chr1.2:2222459-2222478 MS.gene27301:CDS 30.0%
! TTAATTTTCCCTTTCCTCTT+AGG + chr1.2:2222767-2222786 None:intergenic 30.0%
!! ACATGAGTTTTATGAGGATT+TGG - chr1.2:2222616-2222635 MS.gene27301:CDS 30.0%
!! ATTTTAGTGAGGATTTATGG+AGG - chr1.2:2222408-2222427 MS.gene27301:CDS 30.0%
!! TGGATTTTAGTGAGGATTTA+TGG - chr1.2:2222405-2222424 MS.gene27301:CDS 30.0%
AAAGAGATGTGTAGATTTGC+TGG - chr1.2:2222524-2222543 MS.gene27301:CDS 35.0%
AGGAAGAAGGAGAAAATGAA+AGG - chr1.2:2222479-2222498 MS.gene27301:CDS 35.0%
ATGAGGATTTGGAGAAAATG+AGG - chr1.2:2222627-2222646 MS.gene27301:CDS 35.0%
GATTTATGGGCTTGATCTAT+CGG - chr1.2:2222793-2222812 MS.gene27301:CDS 35.0%
GGAAGAAGGAGAAAATGAAA+GGG - chr1.2:2222480-2222499 MS.gene27301:CDS 35.0%
TGATCTATCGGATCCTAAAT+GGG - chr1.2:2222805-2222824 MS.gene27301:CDS 35.0%
TTGATCTATCGGATCCTAAA+TGG - chr1.2:2222804-2222823 MS.gene27301:CDS 35.0%
! ACGGTACTTTTATCACATTC+GGG + chr1.2:2222278-2222297 None:intergenic 35.0%
! CTTCAACGACAGAATTTTCT+CGG + chr1.2:2222918-2222937 None:intergenic 35.0%
! GAAAATGTTGTTGTTGATGC+TGG - chr1.2:2222695-2222714 MS.gene27301:CDS 35.0%
! GGAGGTTAGTAATAAGGTTT+TGG - chr1.2:2222439-2222458 MS.gene27301:CDS 35.0%
! GGTTGTTTCAGAAATGGAAA+AGG - chr1.2:2222328-2222347 MS.gene27301:CDS 35.0%
! TGAAAGGGTTGTTTCAGAAA+TGG - chr1.2:2222322-2222341 MS.gene27301:CDS 35.0%
!! GCTGCTTGTTATTGTTGAAA+GGG - chr1.2:2222307-2222326 MS.gene27301:CDS 35.0%
!! TGCTGCTTGTTATTGTTGAA+AGG - chr1.2:2222306-2222325 MS.gene27301:CDS 35.0%
AACAAGCAGCATTGCATCTA+CGG + chr1.2:2222297-2222316 None:intergenic 40.0%
AATTCTGTCGTTGAAGGAGA+AGG - chr1.2:2222922-2222941 MS.gene27301:CDS 40.0%
AGTGGCTGATAAGATTCATG+AGG - chr1.2:2222832-2222851 MS.gene27301:CDS 40.0%
CGAGAAAATTCTGTCGTTGA+AGG - chr1.2:2222916-2222935 MS.gene27301:CDS 40.0%
TAAATGGGCACGTGAGAAAA+TGG - chr1.2:2222589-2222608 MS.gene27301:CDS 40.0%
TCAGAAATGGAAAAGGGTAG+TGG - chr1.2:2222335-2222354 MS.gene27301:CDS 40.0%
TGCATCTTCGCGATTTGATT+CGG - chr1.2:2222382-2222401 MS.gene27301:CDS 40.0%
TGTGTGGGAGGTTAGTAATA+AGG - chr1.2:2222433-2222452 MS.gene27301:CDS 40.0%
TGTTTGCATTTCCCGGTTAT+CGG + chr1.2:2222890-2222909 None:intergenic 40.0%
! CGGTACTTTTATCACATTCG+GGG + chr1.2:2222277-2222296 None:intergenic 40.0%
! TGGTAATGTTGAGGAGAAGA+AGG - chr1.2:2222730-2222749 MS.gene27301:CDS 40.0%
! TTGGTCTTCCTAAGAGGAAA+GGG - chr1.2:2222756-2222775 MS.gene27301:CDS 40.0%
!! AGATGATAGTTTGTTGACCC+TGG - chr1.2:2222949-2222968 MS.gene27301:CDS 40.0%
!! GGAGGAACATGAGTTTTATG+AGG - chr1.2:2222610-2222629 MS.gene27301:CDS 40.0%
!! GGTAACTTGTTTGCATTTCC+CGG + chr1.2:2222897-2222916 None:intergenic 40.0%
!! GTAGATTTGCTGGTGAAGTT+GGG - chr1.2:2222534-2222553 MS.gene27301:CDS 40.0%
!! TCGTGTTGATGGTAATGTTG+AGG - chr1.2:2222721-2222740 MS.gene27301:CDS 40.0%
!! TGTAGATTTGCTGGTGAAGT+TGG - chr1.2:2222533-2222552 MS.gene27301:CDS 40.0%
!!! TGATTCGGTGGATTTTAGTG+AGG - chr1.2:2222397-2222416 MS.gene27301:CDS 40.0%
AGAAGTTATTTGGCCGAAGG+AGG - chr1.2:2222859-2222878 MS.gene27301:CDS 45.0%
AGAGCTTCTACAGCCTACTA+GGG - chr1.2:2222985-2223004 MS.gene27301:CDS 45.0%
AGGATTTATGGAGGACTGTG+TGG - chr1.2:2222417-2222436 MS.gene27301:CDS 45.0%
ATGAGGCGGGAGAAGTTATT+TGG - chr1.2:2222849-2222868 MS.gene27301:CDS 45.0%
GCTGATAAGATTCATGAGGC+GGG - chr1.2:2222836-2222855 MS.gene27301:CDS 45.0%
GGATCCTAAATGGGAACAAG+TGG - chr1.2:2222814-2222833 MS.gene27301:CDS 45.0%
GGATTTATGGAGGACTGTGT+GGG - chr1.2:2222418-2222437 MS.gene27301:CDS 45.0%
GGCTGATAAGATTCATGAGG+CGG - chr1.2:2222835-2222854 MS.gene27301:CDS 45.0%
GTTGAGGAGAAGAAGGTTGT+TGG - chr1.2:2222737-2222756 MS.gene27301:CDS 45.0%
TAGAGCTTCTACAGCCTACT+AGG - chr1.2:2222984-2223003 MS.gene27301:CDS 45.0%
TCAGCCACTTGTTCCCATTT+AGG + chr1.2:2222821-2222840 None:intergenic 45.0%
TTGATCCAATCCACCCTAGT+AGG + chr1.2:2223001-2223020 None:intergenic 45.0%
! ATGGAAAAGGGTAGTGGTAG+TGG - chr1.2:2222341-2222360 MS.gene27301:CDS 45.0%
! GCGATTTGCTTAGAGAGCTT+AGG - chr1.2:2222564-2222583 MS.gene27301:CDS 45.0%
! TGGAAAAGGGTAGTGGTAGT+GGG - chr1.2:2222342-2222361 MS.gene27301:CDS 45.0%
!! AGGTTGTTGGTCTTCCTAAG+AGG - chr1.2:2222750-2222769 MS.gene27301:CDS 45.0%
!! ATCTTCGCGATTTGATTCGG+TGG - chr1.2:2222385-2222404 MS.gene27301:CDS 45.0%
!! GTTGGTCTTCCTAAGAGGAA+AGG - chr1.2:2222755-2222774 MS.gene27301:CDS 45.0%
ATGGGCACGTGAGAAAATGG+AGG - chr1.2:2222592-2222611 MS.gene27301:CDS 50.0%
CTCTACCCATTCAGCTACCA+GGG + chr1.2:2222969-2222988 None:intergenic 50.0%
CTGTCGTTGAAGGAGAAGGA+TGG - chr1.2:2222926-2222945 MS.gene27301:CDS 50.0%
GATGCTGGTGTTCGTGTTGA+TGG - chr1.2:2222710-2222729 MS.gene27301:CDS 50.0%
GGGAGAAGTTATTTGGCCGA+AGG - chr1.2:2222856-2222875 MS.gene27301:CDS 50.0%
GTTGACCCTGGTAGCTGAAT+GGG - chr1.2:2222961-2222980 MS.gene27301:CDS 50.0%
TACAGCCTACTAGGGTGGAT+TGG - chr1.2:2222993-2223012 MS.gene27301:CDS 50.0%
TGTTGACCCTGGTAGCTGAA+TGG - chr1.2:2222960-2222979 MS.gene27301:CDS 50.0%
TTTATGGAGGACTGTGTGGG+AGG - chr1.2:2222421-2222440 MS.gene27301:CDS 50.0%
! GGAAAAGGGTAGTGGTAGTG+GGG - chr1.2:2222343-2222362 MS.gene27301:CDS 50.0%
! TAGTGGGGGTGGTGATGAAA+AGG - chr1.2:2222358-2222377 MS.gene27301:CDS 50.0%
!! GAAAAGGGTAGTGGTAGTGG+GGG - chr1.2:2222344-2222363 MS.gene27301:CDS 50.0%
AAGGAGGCGAAGCCGATAAC+CGG - chr1.2:2222875-2222894 MS.gene27301:CDS 55.0%
GCTCTACCCATTCAGCTACC+AGG + chr1.2:2222970-2222989 None:intergenic 55.0%
GCTGGTGAAGTTGGGATTCG+AGG - chr1.2:2222542-2222561 MS.gene27301:CDS 55.0%
GCTTCTACAGCCTACTAGGG+TGG - chr1.2:2222988-2223007 MS.gene27301:CDS 55.0%
!! GTTATCGGCTTCGCCTCCTT+CGG + chr1.2:2222875-2222894 None:intergenic 55.0%
AGGAGGCGAAGCCGATAACC+GGG - chr1.2:2222876-2222895 MS.gene27301:CDS 60.0%
!! AAGGGTAGTGGTAGTGGGGG+TGG - chr1.2:2222347-2222366 MS.gene27301:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 2222248 2223090 2222248 ID=MS.gene27301
chr1.2 mRNA 2222248 2223090 2222248 ID=MS.gene27301.t1;Parent=MS.gene27301
chr1.2 exon 2222248 2223090 2222248 ID=MS.gene27301.t1.exon1;Parent=MS.gene27301.t1
chr1.2 CDS 2222248 2223090 2222248 ID=cds.MS.gene27301.t1;Parent=MS.gene27301.t1
Gene Sequence

>MS.gene27301

ATGCGAAAAAAAGTTAAAGAAGTTTACCCCGAATGTGATAAAAGTACCGTAGATGCAATGCTGCTTGTTATTGTTGAAAGGGTTGTTTCAGAAATGGAAAAGGGTAGTGGTAGTGGGGGTGGTGATGAAAAGGTTGCATCTTCGCGATTTGATTCGGTGGATTTTAGTGAGGATTTATGGAGGACTGTGTGGGAGGTTAGTAATAAGGTTTTGGTTGATATGAATAAAGAGAGGAAGAAGGAGAAAATGAAAGGGTTTTTGCAGTGTGATGAAGTGAAAGAGATGTGTAGATTTGCTGGTGAAGTTGGGATTCGAGGCGATTTGCTTAGAGAGCTTAGGTTTAAATGGGCACGTGAGAAAATGGAGGAACATGAGTTTTATGAGGATTTGGAGAAAATGAGGAAAGAGACTCAGATTGTTGATGAAGAAATAAAAAATGAAACCGAAGAAAATGTTGTTGTTGATGCTGGTGTTCGTGTTGATGGTAATGTTGAGGAGAAGAAGGTTGTTGGTCTTCCTAAGAGGAAAGGGAAAATTAAGTTCAAGATTTATGGGCTTGATCTATCGGATCCTAAATGGGAACAAGTGGCTGATAAGATTCATGAGGCGGGAGAAGTTATTTGGCCGAAGGAGGCGAAGCCGATAACCGGGAAATGCAAACAAGTTACCGAGAAAATTCTGTCGTTGAAGGAGAAGGATGGAGATGATAGTTTGTTGACCCTGGTAGCTGAATGGGTAGAGCTTCTACAGCCTACTAGGGTGGATTGGATCAATTTGCTTGAAAGGTTGAAAAATCAGAATTATCCTTTATACTTCAAGGTAACTGTTGCATTGCCTTTTTAG

Protein sequence

>MS.gene27301.t1

MRKKVKEVYPECDKSTVDAMLLVIVERVVSEMEKGSGSGGGDEKVASSRFDSVDFSEDLWRTVWEVSNKVLVDMNKERKKEKMKGFLQCDEVKEMCRFAGEVGIRGDLLRELRFKWAREKMEEHEFYEDLEKMRKETQIVDEEIKNETEENVVVDAGVRVDGNVEEKKVVGLPKRKGKIKFKIYGLDLSDPKWEQVADKIHEAGEVIWPKEAKPITGKCKQVTEKILSLKEKDGDDSLLTLVAEWVELLQPTRVDWINLLERLKNQNYPLYFKVTVALPF