Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30666.t1 | XP_013442954.1 | 92.2 | 230 | 5 | 1 | 1 | 217 | 1 | 230 | 1.50E-119 | 438.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30666.t1 | P08688 | 64.5 | 231 | 68 | 2 | 1 | 217 | 1 | 231 | 1.2e-83 | 310.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30666.t1 | A0A072THF7 | 92.2 | 230 | 5 | 1 | 1 | 217 | 1 | 230 | 1.1e-119 | 438.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene30666 | 0.812817 | 3.50E-51 | -1.69E-46 |
| MS.gene050133 | MS.gene30666 | 0.800584 | 1.35E-48 | -1.69E-46 |
| MS.gene050329 | MS.gene30666 | 0.806424 | 8.28E-50 | -1.69E-46 |
| MS.gene050344 | MS.gene30666 | 0.814498 | 1.49E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene30666 | 0.808156 | 3.56E-50 | -1.69E-46 |
| MS.gene051996 | MS.gene30666 | 0.854697 | 1.04E-61 | -1.69E-46 |
| MS.gene052011 | MS.gene30666 | 0.808374 | 3.20E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene30666 | 0.818536 | 1.86E-52 | -1.69E-46 |
| MS.gene052223 | MS.gene30666 | 0.846886 | 1.64E-59 | -1.69E-46 |
| MS.gene05233 | MS.gene30666 | 0.801964 | 7.02E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene30666 | 0.847236 | 1.31E-59 | -1.69E-46 |
| MS.gene052705 | MS.gene30666 | 0.812791 | 3.55E-51 | -1.69E-46 |
| MS.gene052706 | MS.gene30666 | 0.822785 | 1.97E-53 | -1.69E-46 |
| MS.gene053406 | MS.gene30666 | 0.844127 | 9.16E-59 | -1.69E-46 |
| MS.gene054146 | MS.gene30666 | 0.823589 | 1.28E-53 | -1.69E-46 |
| MS.gene054147 | MS.gene30666 | 0.805475 | 1.31E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene30666 | 0.850213 | 1.96E-60 | -1.69E-46 |
| MS.gene054742 | MS.gene30666 | 0.860935 | 1.46E-63 | -1.69E-46 |
| MS.gene055240 | MS.gene30666 | 0.812321 | 4.50E-51 | -1.69E-46 |
| MS.gene055601 | MS.gene30666 | 0.828293 | 9.75E-55 | -1.69E-46 |
| MS.gene056076 | MS.gene30666 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene30666 | 0.85746 | 1.61E-62 | -1.69E-46 |
| MS.gene056590 | MS.gene30666 | 0.816317 | 5.88E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene30666 | 0.816532 | 5.27E-52 | -1.69E-46 |
| MS.gene056680 | MS.gene30666 | 0.82873 | 7.64E-55 | -1.69E-46 |
| MS.gene056690 | MS.gene30666 | 0.813512 | 2.46E-51 | -1.69E-46 |
| MS.gene056691 | MS.gene30666 | 0.823129 | 1.64E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene30666 | 0.824649 | 7.20E-54 | -1.69E-46 |
| MS.gene056920 | MS.gene30666 | 0.876868 | 9.91E-69 | -1.69E-46 |
| MS.gene056922 | MS.gene30666 | 0.816592 | 5.11E-52 | -1.69E-46 |
| MS.gene056923 | MS.gene30666 | 0.854548 | 1.14E-61 | -1.69E-46 |
| MS.gene056924 | MS.gene30666 | 0.845911 | 3.02E-59 | -1.69E-46 |
| MS.gene056925 | MS.gene30666 | 0.80438 | 2.22E-49 | -1.69E-46 |
| MS.gene057261 | MS.gene30666 | 0.81511 | 1.09E-51 | -1.69E-46 |
| MS.gene057544 | MS.gene30666 | 0.815866 | 7.42E-52 | -1.69E-46 |
| MS.gene058177 | MS.gene30666 | 0.817471 | 3.24E-52 | -1.69E-46 |
| MS.gene059043 | MS.gene30666 | 0.807068 | 6.05E-50 | -1.69E-46 |
| MS.gene059328 | MS.gene30666 | 0.810595 | 1.07E-50 | -1.69E-46 |
| MS.gene059891 | MS.gene30666 | 0.800598 | 1.34E-48 | -1.69E-46 |
| MS.gene059892 | MS.gene30666 | 0.808618 | 2.83E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene30666 | 0.801712 | 7.91E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene30666 | 0.865484 | 5.71E-65 | -1.69E-46 |
| MS.gene060205 | MS.gene30666 | 0.801055 | 1.08E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30666.t1 | MTR_0057s0170 | 92.174 | 230 | 5 | 1 | 1 | 217 | 1 | 230 | 8.30e-155 | 428 |
| MS.gene30666.t1 | MTR_0057s0100 | 90.870 | 230 | 8 | 1 | 1 | 217 | 1 | 230 | 1.35e-152 | 422 |
| MS.gene30666.t1 | MTR_6g040290 | 57.511 | 233 | 83 | 3 | 1 | 217 | 1 | 233 | 9.43e-87 | 256 |
| MS.gene30666.t1 | MTR_7g103990 | 57.759 | 232 | 82 | 4 | 1 | 217 | 1 | 231 | 1.13e-85 | 253 |
| MS.gene30666.t1 | MTR_7g012000 | 54.587 | 218 | 58 | 2 | 1 | 205 | 1 | 190 | 6.92e-74 | 223 |
| MS.gene30666.t1 | MTR_0695s0030 | 48.230 | 226 | 102 | 3 | 6 | 217 | 64 | 288 | 3.45e-70 | 217 |
| MS.gene30666.t1 | MTR_8g038070 | 48.230 | 226 | 102 | 3 | 6 | 217 | 218 | 442 | 2.78e-68 | 216 |
| MS.gene30666.t1 | MTR_4g102470 | 59.893 | 187 | 41 | 2 | 1 | 174 | 1 | 166 | 3.14e-68 | 208 |
| MS.gene30666.t1 | MTR_4g102470 | 34.146 | 123 | 76 | 3 | 65 | 186 | 7 | 125 | 2.89e-11 | 61.2 |
| MS.gene30666.t1 | MTR_8g038100 | 48.230 | 226 | 102 | 3 | 6 | 217 | 280 | 504 | 1.02e-66 | 214 |
| MS.gene30666.t1 | MTR_1g066530 | 38.739 | 222 | 120 | 5 | 4 | 213 | 3 | 220 | 2.34e-47 | 155 |
| MS.gene30666.t1 | MTR_6g073540 | 40.088 | 227 | 113 | 7 | 7 | 213 | 6 | 229 | 5.07e-43 | 144 |
| MS.gene30666.t1 | MTR_6g072470 | 43.069 | 202 | 94 | 6 | 7 | 190 | 6 | 204 | 2.78e-42 | 142 |
| MS.gene30666.t1 | MTR_6g048910 | 38.288 | 222 | 118 | 5 | 7 | 213 | 5 | 222 | 1.10e-39 | 136 |
| MS.gene30666.t1 | MTR_6g045077 | 35.294 | 221 | 124 | 5 | 7 | 213 | 5 | 220 | 6.27e-37 | 129 |
| MS.gene30666.t1 | MTR_6g048620 | 34.081 | 223 | 130 | 4 | 7 | 213 | 5 | 226 | 1.66e-34 | 122 |
| MS.gene30666.t1 | MTR_0807s0020 | 32.883 | 222 | 125 | 6 | 7 | 213 | 6 | 218 | 8.72e-33 | 118 |
| MS.gene30666.t1 | MTR_0216s0030 | 33.163 | 196 | 110 | 5 | 7 | 187 | 3 | 192 | 2.40e-32 | 116 |
| MS.gene30666.t1 | MTR_6g049040 | 43.284 | 134 | 73 | 2 | 7 | 140 | 6 | 136 | 5.93e-29 | 105 |
| MS.gene30666.t1 | MTR_6g049040 | 36.134 | 119 | 51 | 3 | 74 | 179 | 16 | 122 | 5.58e-13 | 63.9 |
| MS.gene30666.t1 | MTR_6g048810 | 26.971 | 241 | 131 | 6 | 7 | 206 | 5 | 241 | 2.08e-20 | 87.4 |
| MS.gene30666.t1 | MTR_6g048810 | 32.258 | 124 | 81 | 2 | 90 | 213 | 180 | 300 | 7.75e-15 | 72.0 |
| MS.gene30666.t1 | MTR_6g048880 | 32.778 | 180 | 99 | 5 | 51 | 213 | 4 | 178 | 3.79e-20 | 84.3 |
| MS.gene30666.t1 | MTR_6g048880 | 32.237 | 152 | 88 | 5 | 16 | 153 | 25 | 175 | 2.82e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATACTTTTTCATCAATGATA+AGG | - | 42999:166-185 | MS.gene30666:CDS | 20.0% |
| ! | AATACAAAATTTGGAAGCAA+TGG | - | 42999:278-297 | MS.gene30666:CDS | 25.0% |
| !! | AATTTTGTATTATCAAGCGA+TGG | + | 42999:269-288 | MS.gene30666:intergenic | 25.0% |
| !! | TCGCTTGATAATACAAAATT+TGG | - | 42999:269-288 | MS.gene30666:CDS | 25.0% |
| !!! | TGAGATTATTTTGTCTTTGT+CGG | + | 42999:390-409 | MS.gene30666:intergenic | 25.0% |
| AAAGAGGTTTATTTGTTCAG+GGG | - | 42999:499-518 | MS.gene30666:intron | 30.0% | |
| AAGATCAGATGACTACATTA+TGG | - | 42999:714-733 | MS.gene30666:CDS | 30.0% | |
| ACAAAGAGGTTTATTTGTTC+AGG | - | 42999:497-516 | MS.gene30666:intron | 30.0% | |
| AGATCAGATGACTACATTAT+GGG | - | 42999:715-734 | MS.gene30666:CDS | 30.0% | |
| ATACAAAATCTTATCGTTGC+TGG | + | 42999:219-238 | MS.gene30666:intergenic | 30.0% | |
| CAAAGAGGTTTATTTGTTCA+GGG | - | 42999:498-517 | MS.gene30666:intron | 30.0% | |
| GACAAAGACAAAATAATCTC+AGG | - | 42999:389-408 | MS.gene30666:CDS | 30.0% | |
| ! | AACATTTCCACGATTTTCAT+AGG | + | 42999:416-435 | MS.gene30666:intergenic | 30.0% |
| ! | AATGAAGCATACCTTTTCAA+AGG | - | 42999:658-677 | MS.gene30666:CDS | 30.0% |
| ! | AGCAACGATAAGATTTTGTA+TGG | - | 42999:218-237 | MS.gene30666:CDS | 30.0% |
| ! | CAAAGTTATCTCCTTTGAAA+AGG | + | 42999:672-691 | MS.gene30666:intergenic | 30.0% |
| ! | TTGAAAAGGTATGCTTCATT+CGG | + | 42999:658-677 | MS.gene30666:intergenic | 30.0% |
| AAAGTATGCTTCATTGTTCC+TGG | + | 42999:153-172 | MS.gene30666:intergenic | 35.0% | |
| AAGTATGCTCGTATAGACTA+TGG | - | 42999:526-545 | MS.gene30666:CDS | 35.0% | |
| ATCAGATGACTACATTATGG+GGG | - | 42999:717-736 | MS.gene30666:CDS | 35.0% | |
| ATTATCAGAGACATCAACAG+CGG | - | 42999:568-587 | MS.gene30666:CDS | 35.0% | |
| GATCAGATGACTACATTATG+GGG | - | 42999:716-735 | MS.gene30666:CDS | 35.0% | |
| ! | CAATGATAAGGTCTTGCTTT+TGG | - | 42999:178-197 | MS.gene30666:CDS | 35.0% |
| ! | GGAAATGTTTCCTTTTTGGA+AGG | - | 42999:427-446 | MS.gene30666:CDS | 35.0% |
| ! | TCGTGGAAATGTTTCCTTTT+TGG | - | 42999:423-442 | MS.gene30666:CDS | 35.0% |
| ! | TGAAAAGGTATGCTTCATTC+GGG | + | 42999:657-676 | MS.gene30666:intergenic | 35.0% |
| !! | TGTTTCCTTTTTGGAAGGAA+CGG | - | 42999:432-451 | MS.gene30666:CDS | 35.0% |
| ATGCTGTATTTCGTTCATCC+AGG | - | 42999:132-151 | MS.gene30666:CDS | 40.0% | |
| ATGTAGTCATCTGATCTTCC+TGG | + | 42999:712-731 | MS.gene30666:intergenic | 40.0% | |
| CAGGTCAACTTTGAACAAAG+AGG | - | 42999:483-502 | MS.gene30666:intron | 40.0% | |
| TCAGATGACTACATTATGGG+GGG | - | 42999:718-737 | MS.gene30666:CDS | 40.0% | |
| TTATCAGAGACATCAACAGC+GGG | - | 42999:569-588 | MS.gene30666:CDS | 40.0% | |
| ! | ATTTTGTCTTTGTCGGTGTG+TGG | + | 42999:383-402 | MS.gene30666:intergenic | 40.0% |
| ! | CCTTTTCAAAGATCGAGTCT+CGG | + | 42999:606-625 | MS.gene30666:intergenic | 40.0% |
| ! | CGAGACTCGATCTTTGAAAA+GGG | - | 42999:604-623 | MS.gene30666:CDS | 40.0% |
| ! | CTTTGAAAAGGGAATCGATG+CGG | - | 42999:615-634 | MS.gene30666:CDS | 40.0% |
| !! | GAGGCATTCGTCTTTTATCA+AGG | - | 42999:332-351 | MS.gene30666:CDS | 40.0% |
| !! | GTTTCCTTTTTGGAAGGAAC+GGG | - | 42999:433-452 | MS.gene30666:CDS | 40.0% |
| !! | TTGCTTTTGGATTATGCTCC+TGG | - | 42999:191-210 | MS.gene30666:CDS | 40.0% |
| !! | TTTCCTTTTTGGAAGGAACG+GGG | - | 42999:434-453 | MS.gene30666:CDS | 40.0% |
| AAACCCCGTTCCTTCCAAAA+AGG | + | 42999:440-459 | None:intergenic | 45.0% | |
| ATGGAATAGACGCTGCATTC+AGG | - | 42999:464-483 | MS.gene30666:intron | 45.0% | |
| CTCAGGTCCTATGAAAATCG+TGG | - | 42999:406-425 | MS.gene30666:CDS | 45.0% | |
| CTCGTTGTCATCAGTGTCAA+AGG | + | 42999:315-334 | MS.gene30666:intergenic | 45.0% | |
| CTTTGACACTGATGACAACG+AGG | - | 42999:313-332 | MS.gene30666:CDS | 45.0% | |
| GGATGTTTGGAAGTCTCTTC+AGG | - | 42999:756-775 | MS.gene30666:CDS | 45.0% | |
| TGAGGTCAACTCTGGATGTT+TGG | - | 42999:743-762 | MS.gene30666:CDS | 45.0% | |
| ! | CCGAGACTCGATCTTTGAAA+AGG | - | 42999:603-622 | MS.gene30666:CDS | 45.0% |
| !! | ATCTTATCGTTGCTGGTTCC+AGG | + | 42999:212-231 | MS.gene30666:intergenic | 45.0% |
| !! | GTGCGTATCACTTTTACACC+AGG | - | 42999:691-710 | MS.gene30666:CDS | 45.0% |
| GAAGGAACGGGGTTTGAACA+TGG | - | 42999:445-464 | MS.gene30666:intron | 50.0% | |
| ! | ACTACATTATGGGGGGTGTG+AGG | - | 42999:725-744 | MS.gene30666:CDS | 50.0% |
| AACCCGTGACGAACGAAAGC+AGG | + | 42999:245-264 | MS.gene30666:intergenic | 55.0% | |
| GACCTGCTTTCGTTCGTCAC+GGG | - | 42999:240-259 | MS.gene30666:CDS | 55.0% | |
| GGACCTGCTTTCGTTCGTCA+CGG | - | 42999:239-258 | MS.gene30666:CDS | 55.0% | |
| ! | GGGGGGTGTGAGGTCAACTC+TGG | - | 42999:735-754 | MS.gene30666:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 42999 | gene | 110 | 801 | 110 | ID=MS.gene30666 |
| 42999 | mRNA | 110 | 801 | 110 | ID=MS.gene30666.t1;Parent=MS.gene30666 |
| 42999 | exon | 501 | 801 | 501 | ID=MS.gene30666.t1.exon1;Parent=MS.gene30666.t1 |
| 42999 | CDS | 501 | 801 | 501 | ID=cds.MS.gene30666.t1;Parent=MS.gene30666.t1 |
| 42999 | exon | 110 | 462 | 110 | ID=MS.gene30666.t1.exon2;Parent=MS.gene30666.t1 |
| 42999 | CDS | 110 | 462 | 110 | ID=cds.MS.gene30666.t1;Parent=MS.gene30666.t1 |
| Gene Sequence |
| Protein sequence |
