Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34312.t1 | XP_013454491.1 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 1.20E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34312.t1 | A0A072UFR3 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 8.9e-84 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene34312 | 0.834438 | 3.00E-56 | -1.69E-46 |
| MS.gene049427 | MS.gene34312 | 0.830705 | 2.53E-55 | -1.69E-46 |
| MS.gene049805 | MS.gene34312 | 0.806037 | 9.99E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene34312 | 0.805597 | 1.24E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene34312 | 0.810764 | 9.80E-51 | -1.69E-46 |
| MS.gene050747 | MS.gene34312 | 0.808999 | 2.35E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene34312 | 0.814887 | 1.23E-51 | -1.69E-46 |
| MS.gene05081 | MS.gene34312 | 0.81115 | 8.08E-51 | -1.69E-46 |
| MS.gene050950 | MS.gene34312 | 0.800896 | 1.16E-48 | -1.69E-46 |
| MS.gene05105 | MS.gene34312 | 0.809805 | 1.58E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene34312 | 0.821657 | 3.59E-53 | -1.69E-46 |
| MS.gene051666 | MS.gene34312 | 0.837512 | 4.98E-57 | -1.69E-46 |
| MS.gene051838 | MS.gene34312 | 0.829162 | 6.01E-55 | -1.69E-46 |
| MS.gene052322 | MS.gene34312 | 0.801886 | 7.29E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene34312 | 0.842138 | 3.10E-58 | -1.69E-46 |
| MS.gene052484 | MS.gene34312 | 0.820622 | 6.22E-53 | -1.69E-46 |
| MS.gene052501 | MS.gene34312 | 0.828752 | 7.55E-55 | -1.69E-46 |
| MS.gene052505 | MS.gene34312 | 0.824522 | 7.71E-54 | -1.69E-46 |
| MS.gene052982 | MS.gene34312 | 0.822506 | 2.28E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene34312 | 0.825238 | 5.23E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene34312 | 0.804971 | 1.67E-49 | -1.69E-46 |
| MS.gene053107 | MS.gene34312 | 0.801848 | 7.42E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene34312 | 0.829714 | 4.41E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene34312 | 0.808727 | 2.69E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene34312 | 0.814001 | 1.92E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene34312 | 0.812261 | 4.63E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene34312 | 0.814395 | 1.57E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene34312 | 0.816722 | 4.78E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene34312 | 0.809405 | 1.92E-50 | -1.69E-46 |
| MS.gene056897 | MS.gene34312 | 0.807809 | 4.22E-50 | -1.69E-46 |
| MS.gene057011 | MS.gene34312 | 0.801867 | 7.35E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene34312 | 0.843369 | 1.46E-58 | -1.69E-46 |
| MS.gene057196 | MS.gene34312 | 0.818185 | 2.24E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene34312 | 0.803165 | 3.97E-49 | -1.69E-46 |
| MS.gene057658 | MS.gene34312 | 0.833642 | 4.74E-56 | -1.69E-46 |
| MS.gene058233 | MS.gene34312 | 0.818115 | 2.32E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene34312 | 0.806032 | 1.00E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene34312 | 0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene059023 | MS.gene34312 | 0.804183 | 2.44E-49 | -1.69E-46 |
| MS.gene05914 | MS.gene34312 | 0.804906 | 1.73E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene34312 | 0.804605 | 1.99E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene34312 | 0.814442 | 1.54E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene34312 | 0.81735 | 3.45E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene34312 | 0.822919 | 1.83E-53 | -1.69E-46 |
| MS.gene060403 | MS.gene34312 | 0.828408 | 9.14E-55 | -1.69E-46 |
| MS.gene060407 | MS.gene34312 | 0.811659 | 6.27E-51 | -1.69E-46 |
| MS.gene06092 | MS.gene34312 | 0.850321 | 1.83E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34312.t1 | MTR_4g005360 | 99.338 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 7.44e-111 | 314 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34312.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
| MS.gene34312.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
Find 35 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGCTGTTCTTGACAATT+TGG | 0.246299 | 4.3:+89154007 | MS.gene34312:CDS |
| ATCTCATCCACTTGTATATC+AGG | 0.333453 | 4.3:-89153542 | None:intergenic |
| ACTGATCTAGTGTAAGGATA+TGG | 0.366614 | 4.3:-89154291 | None:intergenic |
| ATGAACTTGTATCTCGAATC+TGG | 0.411354 | 4.3:+89153985 | MS.gene34312:CDS |
| CCAATACCATCTGCTTGGTT+AGG | 0.422828 | 4.3:+89154420 | MS.gene34312:CDS |
| TATTTGTTGACTTATATGTA+AGG | 0.446098 | 4.3:-89153507 | None:intergenic |
| CCTAACCAAGCAGATGGTAT+TGG | 0.447991 | 4.3:-89154420 | None:intergenic |
| ATACTTCACGGAACCATGAT+TGG | 0.450822 | 4.3:+89153632 | MS.gene34312:CDS |
| TACAAGTGGATGAGATTGTT+GGG | 0.457723 | 4.3:+89153549 | MS.gene34312:CDS |
| TGTTGGCATGTGCTAAAAGT+AGG | 0.469168 | 4.3:+89154391 | MS.gene34312:CDS |
| ACTTTGACTGATCTAGTGTA+AGG | 0.474599 | 4.3:-89154297 | None:intergenic |
| TCCACCATGAGGAGGCATAA+TGG | 0.492329 | 4.3:-89153609 | None:intergenic |
| TTCAGCCAATACCATCTGCT+TGG | 0.495555 | 4.3:+89154415 | MS.gene34312:CDS |
| GCAGATCAGTTTATTGCCAA+TGG | 0.496622 | 4.3:+89153942 | MS.gene34312:intron |
| AGATTCGAGATACAAGTTCA+TGG | 0.497424 | 4.3:-89153983 | None:intergenic |
| CTAGATCAGTCAAAGTTCAA+TGG | 0.503366 | 4.3:+89154304 | MS.gene34312:CDS |
| CCTCATGGTGGAATACTTCA+CGG | 0.510920 | 4.3:+89153620 | MS.gene34312:CDS |
| TACGTCTCTTGACTTGCCAT+TGG | 0.513346 | 4.3:-89153958 | None:intergenic |
| TGTAGATTATTATGATATGT+TGG | 0.518385 | 4.3:+89154374 | MS.gene34312:CDS |
| CTTAAACGCCTTGCACATGA+AGG | 0.518985 | 4.3:+89154056 | MS.gene34312:CDS |
| AAGGCGTTTAAGTAAGCAAA+AGG | 0.541095 | 4.3:-89154045 | None:intergenic |
| GGGGCATGCCATACCAATCA+TGG | 0.551161 | 4.3:-89153645 | None:intergenic |
| CTTTCCATTATGCCTCCTCA+TGG | 0.553623 | 4.3:+89153605 | MS.gene34312:CDS |
| ATACAAGTGGATGAGATTGT+TGG | 0.562800 | 4.3:+89153548 | MS.gene34312:CDS |
| CTACATGGTTGAAGCAATCA+CGG | 0.564717 | 4.3:-89154356 | None:intergenic |
| GATTCGAGATACAAGTTCAT+GGG | 0.566517 | 4.3:-89153982 | None:intergenic |
| GATCAGTCAAAGTTCAATGG+AGG | 0.590714 | 4.3:+89154307 | MS.gene34312:CDS |
| ACATATCATAATAATCTACA+TGG | 0.601342 | 4.3:-89154371 | None:intergenic |
| ATCAGTCAAAGTTCAATGGA+GGG | 0.611627 | 4.3:+89154308 | MS.gene34312:CDS |
| TAATAACCTAACCAAGCAGA+TGG | 0.613735 | 4.3:-89154426 | None:intergenic |
| TTACATCTCCTTCATGTGCA+AGG | 0.614326 | 4.3:-89154064 | None:intergenic |
| TCCATTATGCCTCCTCATGG+TGG | 0.618370 | 4.3:+89153608 | MS.gene34312:CDS |
| TTCTGATCCTGATATACAAG+TGG | 0.625145 | 4.3:+89153535 | MS.gene34312:CDS |
| TGAAGTATTCCACCATGAGG+AGG | 0.648479 | 4.3:-89153617 | None:intergenic |
| CCGTGAAGTATTCCACCATG+AGG | 0.714100 | 4.3:-89153620 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCATAATAATCTACA+TGG | - | chr4.3:89154374-89154393 | None:intergenic | 20.0% |
| !! | TGTAGATTATTATGATATGT+TGG | + | chr4.3:89154374-89154393 | MS.gene34312:CDS | 20.0% |
| !! | TTCATTTCAATTAGAATCAT+AGG | - | chr4.3:89153815-89153834 | None:intergenic | 20.0% |
| !!! | TATTTGTTGACTTATATGTA+AGG | - | chr4.3:89153510-89153529 | None:intergenic | 20.0% |
| ! | ATTTCTTAGATTTGTGCAAA+AGG | + | chr4.3:89154159-89154178 | MS.gene34312:intron | 25.0% |
| ! | TGTAACAACAAAAGTAAAGA+AGG | - | chr4.3:89153672-89153691 | None:intergenic | 25.0% |
| !! | AATGAATTAGTTCTCTAAGT+TGG | + | chr4.3:89153829-89153848 | MS.gene34312:intron | 25.0% |
| !! | CTTATATCTTTATGTCCTTT+TGG | + | chr4.3:89153692-89153711 | MS.gene34312:intron | 25.0% |
| AAAGAGACAAAATGTTGATG+AGG | - | chr4.3:89154227-89154246 | None:intergenic | 30.0% | |
| AACAACAAAAGTAAAGAAGG+TGG | - | chr4.3:89153669-89153688 | None:intergenic | 30.0% | |
| ACAACAAAAGTAAAGAAGGT+GGG | - | chr4.3:89153668-89153687 | None:intergenic | 30.0% | |
| AGGATCAATTCAGTTTGATA+TGG | - | chr4.3:89153795-89153814 | None:intergenic | 30.0% | |
| TTGGTTTATCTTGTTCTTGT+TGG | + | chr4.3:89153848-89153867 | MS.gene34312:intron | 30.0% | |
| ! | TTTTTGCAATAACTTTCAGC+AGG | + | chr4.3:89154260-89154279 | MS.gene34312:intron | 30.0% |
| ACTCGACATAAGAGTTGTTA+CGG | - | chr4.3:89153724-89153743 | None:intergenic | 35.0% | |
| ACTGATCTAGTGTAAGGATA+TGG | - | chr4.3:89154294-89154313 | None:intergenic | 35.0% | |
| ACTTTGACTGATCTAGTGTA+AGG | - | chr4.3:89154300-89154319 | None:intergenic | 35.0% | |
| AGATTCGAGATACAAGTTCA+TGG | - | chr4.3:89153986-89154005 | None:intergenic | 35.0% | |
| ATACAAGTGGATGAGATTGT+TGG | + | chr4.3:89153548-89153567 | MS.gene34312:CDS | 35.0% | |
| ATCAGTCAAAGTTCAATGGA+GGG | + | chr4.3:89154308-89154327 | MS.gene34312:CDS | 35.0% | |
| ATCTCATCCACTTGTATATC+AGG | - | chr4.3:89153545-89153564 | None:intergenic | 35.0% | |
| ATGAACTTGTATCTCGAATC+TGG | + | chr4.3:89153985-89154004 | MS.gene34312:CDS | 35.0% | |
| CAACAAAAGTAAAGAAGGTG+GGG | - | chr4.3:89153667-89153686 | None:intergenic | 35.0% | |
| CTAGATCAGTCAAAGTTCAA+TGG | + | chr4.3:89154304-89154323 | MS.gene34312:CDS | 35.0% | |
| GATTCGAGATACAAGTTCAT+GGG | - | chr4.3:89153985-89154004 | None:intergenic | 35.0% | |
| TAATAACCTAACCAAGCAGA+TGG | - | chr4.3:89154429-89154448 | None:intergenic | 35.0% | |
| TACAAGTGGATGAGATTGTT+GGG | + | chr4.3:89153549-89153568 | MS.gene34312:CDS | 35.0% | |
| TTCTGATCCTGATATACAAG+TGG | + | chr4.3:89153535-89153554 | MS.gene34312:CDS | 35.0% | |
| TTGTAGGAGAGAGGTTAATA+TGG | + | chr4.3:89153745-89153764 | MS.gene34312:intron | 35.0% | |
| TTGTTACGGGATAAACCAAA+AGG | - | chr4.3:89153710-89153729 | None:intergenic | 35.0% | |
| ! | AAGGCGTTTAAGTAAGCAAA+AGG | - | chr4.3:89154048-89154067 | None:intergenic | 35.0% |
| !! | ACTCTTATGTCGAGTTTTGT+AGG | + | chr4.3:89153729-89153748 | MS.gene34312:intron | 35.0% |
| ATACTTCACGGAACCATGAT+TGG | + | chr4.3:89153632-89153651 | MS.gene34312:CDS | 40.0% | |
| CTACATGGTTGAAGCAATCA+CGG | - | chr4.3:89154359-89154378 | None:intergenic | 40.0% | |
| CTCGACATAAGAGTTGTTAC+GGG | - | chr4.3:89153723-89153742 | None:intergenic | 40.0% | |
| GATCAGTCAAAGTTCAATGG+AGG | + | chr4.3:89154307-89154326 | MS.gene34312:CDS | 40.0% | |
| GCAGATCAGTTTATTGCCAA+TGG | + | chr4.3:89153942-89153961 | MS.gene34312:intron | 40.0% | |
| TGTTGGCATGTGCTAAAAGT+AGG | + | chr4.3:89154391-89154410 | MS.gene34312:CDS | 40.0% | |
| TTACATCTCCTTCATGTGCA+AGG | - | chr4.3:89154067-89154086 | None:intergenic | 40.0% | |
| !! | GCTTGCTGTTCTTGACAATT+TGG | + | chr4.3:89154007-89154026 | MS.gene34312:CDS | 40.0% |
| CCAATACCATCTGCTTGGTT+AGG | + | chr4.3:89154420-89154439 | MS.gene34312:CDS | 45.0% | |
| CCTAACCAAGCAGATGGTAT+TGG | - | chr4.3:89154423-89154442 | None:intergenic | 45.0% | |
| CTTAAACGCCTTGCACATGA+AGG | + | chr4.3:89154056-89154075 | MS.gene34312:CDS | 45.0% | |
| CTTTCCATTATGCCTCCTCA+TGG | + | chr4.3:89153605-89153624 | MS.gene34312:CDS | 45.0% | |
| TCACGGAACCATGATTGGTA+TGG | + | chr4.3:89153637-89153656 | MS.gene34312:intron | 45.0% | |
| TTCAGCCAATACCATCTGCT+TGG | + | chr4.3:89154415-89154434 | MS.gene34312:CDS | 45.0% | |
| ! | CCTCATGGTGGAATACTTCA+CGG | + | chr4.3:89153620-89153639 | MS.gene34312:CDS | 45.0% |
| ! | TGAAGTATTCCACCATGAGG+AGG | - | chr4.3:89153620-89153639 | None:intergenic | 45.0% |
| !! | TACGTCTCTTGACTTGCCAT+TGG | - | chr4.3:89153961-89153980 | None:intergenic | 45.0% |
| !! | TGTCGAGTTTTGTAGGAGAG+AGG | + | chr4.3:89153736-89153755 | MS.gene34312:intron | 45.0% |
| !! | AAATAATGATAAAAATATAT+AGG | - | chr4.3:89154143-89154162 | None:intergenic | 5.0% |
| CGCCCATAATCTAGCAAAGG+CGG | + | chr4.3:89153769-89153788 | MS.gene34312:intron | 50.0% | |
| GGCCGCCTTTGCTAGATTAT+GGG | - | chr4.3:89153774-89153793 | None:intergenic | 50.0% | |
| GGTCGCCCATAATCTAGCAA+AGG | + | chr4.3:89153766-89153785 | MS.gene34312:intron | 50.0% | |
| TCCATTATGCCTCCTCATGG+TGG | + | chr4.3:89153608-89153627 | MS.gene34312:CDS | 50.0% | |
| TGGCCGCCTTTGCTAGATTA+TGG | - | chr4.3:89153775-89153794 | None:intergenic | 50.0% | |
| ! | CCGTGAAGTATTCCACCATG+AGG | - | chr4.3:89153623-89153642 | None:intergenic | 50.0% |
| ! | TCCACCATGAGGAGGCATAA+TGG | - | chr4.3:89153612-89153631 | None:intergenic | 50.0% |
| GGGGCATGCCATACCAATCA+TGG | - | chr4.3:89153648-89153667 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89153503 | 89154449 | 89153503 | ID=MS.gene34312 |
| chr4.3 | mRNA | 89153503 | 89154449 | 89153503 | ID=MS.gene34312.t1;Parent=MS.gene34312 |
| chr4.3 | exon | 89153503 | 89153653 | 89153503 | ID=MS.gene34312.t1.exon1;Parent=MS.gene34312.t1 |
| chr4.3 | CDS | 89153503 | 89153653 | 89153503 | ID=cds.MS.gene34312.t1;Parent=MS.gene34312.t1 |
| chr4.3 | exon | 89153946 | 89154082 | 89153946 | ID=MS.gene34312.t1.exon2;Parent=MS.gene34312.t1 |
| chr4.3 | CDS | 89153946 | 89154082 | 89153946 | ID=cds.MS.gene34312.t1;Parent=MS.gene34312.t1 |
| chr4.3 | exon | 89154282 | 89154449 | 89154282 | ID=MS.gene34312.t1.exon3;Parent=MS.gene34312.t1 |
| chr4.3 | CDS | 89154282 | 89154449 | 89154282 | ID=cds.MS.gene34312.t1;Parent=MS.gene34312.t1 |
| Gene Sequence |
| Protein sequence |
