Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38152.t1 | XP_003597487.1 | 86.1 | 553 | 65 | 2 | 1 | 553 | 1 | 541 | 9.70E-267 | 929.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38152.t1 | Q84RR0 | 63.3 | 551 | 178 | 3 | 1 | 550 | 1 | 528 | 7.9e-209 | 728.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38152.t1 | G7IHR6 | 86.1 | 553 | 65 | 2 | 1 | 553 | 1 | 541 | 7.0e-267 | 929.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene38152 | 0.832264 | 1.04E-55 | -1.69E-46 |
| MS.gene049404 | MS.gene38152 | 0.839138 | 1.90E-57 | -1.69E-46 |
| MS.gene049701 | MS.gene38152 | 0.807179 | 5.73E-50 | -1.69E-46 |
| MS.gene05016 | MS.gene38152 | 0.803828 | 2.89E-49 | -1.69E-46 |
| MS.gene050273 | MS.gene38152 | 0.814758 | 1.31E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene38152 | 0.8242 | 9.18E-54 | -1.69E-46 |
| MS.gene050561 | MS.gene38152 | 0.822328 | 2.51E-53 | -1.69E-46 |
| MS.gene050562 | MS.gene38152 | 0.814327 | 1.63E-51 | -1.69E-46 |
| MS.gene051181 | MS.gene38152 | 0.809726 | 1.64E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene38152 | 0.802764 | 4.80E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene38152 | 0.850732 | 1.40E-60 | -1.69E-46 |
| MS.gene051719 | MS.gene38152 | 0.808363 | 3.21E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene38152 | 0.801829 | 7.48E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene38152 | 0.840772 | 7.11E-58 | -1.69E-46 |
| MS.gene051957 | MS.gene38152 | 0.803325 | 3.68E-49 | -1.69E-46 |
| MS.gene052488 | MS.gene38152 | 0.802767 | 4.80E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene38152 | 0.811749 | 5.99E-51 | -1.69E-46 |
| MS.gene052611 | MS.gene38152 | 0.802054 | 6.73E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene38152 | 0.8193 | 1.25E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene38152 | 0.807528 | 4.84E-50 | -1.69E-46 |
| MS.gene053974 | MS.gene38152 | 0.800589 | 1.34E-48 | -1.69E-46 |
| MS.gene053998 | MS.gene38152 | 0.81558 | 8.59E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene38152 | 0.811542 | 6.65E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene38152 | 0.80788 | 4.07E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene38152 | 0.801496 | 8.76E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene38152 | 0.804656 | 1.95E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene38152 | 0.832224 | 1.07E-55 | -1.69E-46 |
| MS.gene055227 | MS.gene38152 | 0.805395 | 1.36E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene38152 | 0.828963 | 6.71E-55 | -1.69E-46 |
| MS.gene055818 | MS.gene38152 | 0.823695 | 1.21E-53 | -1.69E-46 |
| MS.gene05604 | MS.gene38152 | 0.815558 | 8.69E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene38152 | 0.826284 | 2.95E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene38152 | 0.830972 | 2.17E-55 | -1.69E-46 |
| MS.gene056366 | MS.gene38152 | 0.822594 | 2.18E-53 | -1.69E-46 |
| MS.gene056553 | MS.gene38152 | 0.811347 | 7.33E-51 | -1.69E-46 |
| MS.gene05686 | MS.gene38152 | 0.810337 | 1.21E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene38152 | 0.801403 | 9.15E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene38152 | 0.828724 | 7.67E-55 | -1.69E-46 |
| MS.gene057869 | MS.gene38152 | 0.800847 | 1.19E-48 | -1.69E-46 |
| MS.gene058010 | MS.gene38152 | 0.833562 | 4.97E-56 | -1.69E-46 |
| MS.gene058294 | MS.gene38152 | 0.830445 | 2.93E-55 | -1.69E-46 |
| MS.gene058515 | MS.gene38152 | 0.814394 | 1.58E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene38152 | 0.815619 | 8.42E-52 | -1.69E-46 |
| MS.gene058979 | MS.gene38152 | 0.821519 | 3.87E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene38152 | 0.831929 | 1.26E-55 | -1.69E-46 |
| MS.gene059272 | MS.gene38152 | 0.80514 | 1.54E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene38152 | 0.830551 | 2.76E-55 | -1.69E-46 |
| MS.gene060167 | MS.gene38152 | 0.804854 | 1.77E-49 | -1.69E-46 |
| MS.gene060312 | MS.gene38152 | 0.805821 | 1.11E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38152.t1 | MTR_2g098580 | 84.685 | 555 | 73 | 2 | 1 | 555 | 1 | 543 | 0.0 | 946 |
| MS.gene38152.t1 | MTR_3g065380 | 77.500 | 560 | 86 | 5 | 1 | 555 | 1 | 525 | 0.0 | 861 |
| MS.gene38152.t1 | MTR_4g066650 | 74.327 | 557 | 117 | 5 | 1 | 555 | 1 | 533 | 0.0 | 823 |
| MS.gene38152.t1 | MTR_3g065410 | 75.812 | 554 | 92 | 5 | 1 | 553 | 1 | 513 | 0.0 | 812 |
| MS.gene38152.t1 | MTR_5g097670 | 91.925 | 322 | 16 | 1 | 235 | 556 | 4 | 315 | 0.0 | 615 |
| MS.gene38152.t1 | MTR_4g075010 | 40.619 | 485 | 209 | 13 | 69 | 538 | 2 | 422 | 3.59e-111 | 343 |
| MS.gene38152.t1 | MTR_4g075030 | 38.802 | 384 | 187 | 11 | 41 | 410 | 8 | 357 | 9.78e-75 | 242 |
| MS.gene38152.t1 | MTR_4g068940 | 28.214 | 560 | 339 | 17 | 15 | 551 | 1 | 520 | 1.81e-62 | 216 |
| MS.gene38152.t1 | MTR_4g068940 | 28.214 | 560 | 339 | 17 | 15 | 551 | 1 | 520 | 2.32e-62 | 216 |
| MS.gene38152.t1 | MTR_4g096720 | 54.545 | 66 | 27 | 1 | 255 | 317 | 58 | 123 | 2.26e-16 | 77.4 |
| MS.gene38152.t1 | MTR_3g462590 | 25.000 | 232 | 159 | 8 | 118 | 341 | 66 | 290 | 1.24e-14 | 75.1 |
| MS.gene38152.t1 | MTR_3g462570 | 27.826 | 230 | 141 | 11 | 136 | 355 | 33 | 247 | 1.87e-12 | 70.5 |
| MS.gene38152.t1 | MTR_2g044420 | 26.829 | 205 | 140 | 7 | 127 | 325 | 65 | 265 | 5.98e-12 | 67.0 |
| MS.gene38152.t1 | MTR_5g078410 | 25.862 | 232 | 129 | 8 | 125 | 341 | 127 | 330 | 2.60e-11 | 65.9 |
| MS.gene38152.t1 | MTR_3g014030 | 26.549 | 226 | 147 | 10 | 141 | 355 | 1510 | 1727 | 7.13e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38152.t1 | AT2G31510 | 65.996 | 547 | 162 | 4 | 1 | 546 | 1 | 524 | 0.0 | 748 |
| MS.gene38152.t1 | AT1G65430 | 65.989 | 541 | 163 | 5 | 16 | 554 | 4 | 525 | 0.0 | 722 |
| MS.gene38152.t1 | AT1G05890 | 63.806 | 536 | 167 | 4 | 18 | 553 | 16 | 524 | 0.0 | 719 |
| MS.gene38152.t1 | AT2G31510 | 65.451 | 521 | 156 | 4 | 1 | 520 | 1 | 498 | 0.0 | 709 |
| MS.gene38152.t1 | AT1G05890 | 67.909 | 483 | 143 | 2 | 71 | 553 | 96 | 566 | 0.0 | 708 |
| MS.gene38152.t1 | AT2G31510 | 70.933 | 461 | 124 | 1 | 86 | 546 | 2 | 452 | 0.0 | 707 |
| MS.gene38152.t1 | AT2G31770 | 53.524 | 525 | 205 | 9 | 33 | 544 | 2 | 500 | 0.0 | 565 |
| MS.gene38152.t1 | AT2G31760 | 52.529 | 514 | 213 | 8 | 31 | 541 | 2 | 487 | 0.0 | 548 |
| MS.gene38152.t1 | AT2G31780 | 50.756 | 463 | 202 | 6 | 1 | 461 | 1 | 439 | 1.46e-165 | 478 |
| MS.gene38152.t1 | AT1G05880 | 36.502 | 526 | 281 | 13 | 39 | 548 | 2 | 490 | 5.04e-99 | 309 |
| MS.gene38152.t1 | AT1G05880 | 36.502 | 526 | 274 | 15 | 39 | 548 | 2 | 483 | 7.49e-95 | 298 |
| MS.gene38152.t1 | AT1G05880 | 36.680 | 488 | 270 | 12 | 39 | 512 | 2 | 464 | 4.53e-92 | 291 |
| MS.gene38152.t1 | AT3G27710 | 31.507 | 511 | 299 | 16 | 49 | 538 | 19 | 499 | 2.05e-66 | 225 |
| MS.gene38152.t1 | AT4G34370 | 32.108 | 517 | 275 | 20 | 59 | 538 | 22 | 499 | 8.71e-66 | 225 |
| MS.gene38152.t1 | AT2G16090 | 29.015 | 548 | 317 | 19 | 15 | 538 | 1 | 500 | 7.93e-62 | 213 |
| MS.gene38152.t1 | AT2G16090 | 29.380 | 548 | 315 | 19 | 15 | 538 | 1 | 500 | 2.01e-61 | 213 |
| MS.gene38152.t1 | AT2G16090 | 29.380 | 548 | 315 | 19 | 15 | 538 | 1 | 500 | 2.24e-61 | 213 |
| MS.gene38152.t1 | AT2G16090 | 30.704 | 469 | 270 | 17 | 94 | 538 | 3 | 440 | 1.07e-56 | 198 |
| MS.gene38152.t1 | AT2G16090 | 30.704 | 469 | 270 | 17 | 94 | 538 | 3 | 440 | 2.10e-56 | 198 |
| MS.gene38152.t1 | AT5G63750 | 27.112 | 509 | 303 | 15 | 72 | 541 | 9 | 488 | 3.98e-50 | 181 |
| MS.gene38152.t1 | AT5G08730 | 26.141 | 482 | 308 | 10 | 72 | 545 | 8 | 449 | 7.02e-49 | 177 |
| MS.gene38152.t1 | AT4G34370 | 34.153 | 366 | 177 | 14 | 199 | 538 | 5 | 332 | 8.98e-48 | 172 |
| MS.gene38152.t1 | AT3G27720 | 33.043 | 345 | 186 | 11 | 211 | 538 | 143 | 459 | 9.25e-46 | 168 |
| MS.gene38152.t1 | AT5G63730 | 25.514 | 486 | 306 | 13 | 72 | 541 | 9 | 454 | 1.46e-38 | 148 |
| MS.gene38152.t1 | AT5G63760 | 25.602 | 457 | 253 | 14 | 125 | 543 | 2 | 409 | 3.25e-37 | 144 |
| MS.gene38152.t1 | AT5G63760 | 25.602 | 457 | 253 | 14 | 125 | 543 | 2 | 409 | 3.25e-37 | 144 |
| MS.gene38152.t1 | AT5G63760 | 25.602 | 457 | 253 | 14 | 125 | 543 | 2 | 409 | 3.25e-37 | 144 |
| MS.gene38152.t1 | AT5G63760 | 26.571 | 350 | 183 | 11 | 220 | 543 | 2 | 303 | 5.41e-29 | 118 |
| MS.gene38152.t1 | AT1G32340 | 25.359 | 209 | 130 | 6 | 149 | 337 | 378 | 580 | 2.20e-11 | 67.0 |
Find 100 sgRNAs with CRISPR-Local
Find 142 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTACTTCGTCATTATAAT+TGG | 0.164956 | 5.3:-79429231 | MS.gene38152:CDS |
| CCCGCCTGCACTCAACTATT+TGG | 0.208083 | 5.3:+79427884 | None:intergenic |
| AAGTATGACCGTTACCTTAT+TGG | 0.236137 | 5.3:-79428887 | MS.gene38152:CDS |
| TCATGCTGGGGAGGATATAT+TGG | 0.292821 | 5.3:-79429025 | MS.gene38152:CDS |
| GCATTAGGATTCTCATAAAC+TGG | 0.344755 | 5.3:+79429133 | None:intergenic |
| CTTCAACGTAACATCCAATA+AGG | 0.350174 | 5.3:+79428873 | None:intergenic |
| TTACGTTGAAGACAATAAAA+AGG | 0.360499 | 5.3:-79428861 | MS.gene38152:intron |
| GCAGCTCTTTGAGTACTTGC+AGG | 0.365534 | 5.3:-79427803 | MS.gene38152:CDS |
| CAGGAGAAAAGTTGGCTTGT+TGG | 0.384380 | 5.3:-79429161 | MS.gene38152:CDS |
| CCATATGAACCGTTTGCATC+TGG | 0.400337 | 5.3:+79427971 | None:intergenic |
| GTCATAGTTGTAGTCATCTT+CGG | 0.407825 | 5.3:+79429471 | None:intergenic |
| CACTTCAATTAACGATGGTC+CGG | 0.414083 | 5.3:-79429002 | MS.gene38152:CDS |
| CCAGATGCAAACGGTTCATA+TGG | 0.415301 | 5.3:-79427971 | MS.gene38152:CDS |
| CTTCAGTACTGAGGAATACT+TGG | 0.423493 | 5.3:+79427728 | None:intergenic |
| CAATACGGCAAAATTTATCT+CGG | 0.434149 | 5.3:+79429339 | None:intergenic |
| CAGAATCCGAAAACACAAAC+TGG | 0.437540 | 5.3:-79428311 | MS.gene38152:CDS |
| AGTAGGATGCTTGCAGCAAC+TGG | 0.437632 | 5.3:+79429247 | None:intergenic |
| AGAAATGGCAAAGAATTCAT+TGG | 0.440218 | 5.3:-79428067 | MS.gene38152:CDS |
| TTCAATCGGTCTCTTACACT+TGG | 0.440721 | 5.3:+79428263 | None:intergenic |
| ACTTCATCAATGCGCGGAAA+AGG | 0.441283 | 5.3:-79427755 | MS.gene38152:CDS |
| GCAGCACCACAAGCGGGATC+AGG | 0.445228 | 5.3:+79428959 | None:intergenic |
| ACCAAGTGGTGTCCTGCTCC+TGG | 0.450476 | 5.3:-79428483 | MS.gene38152:CDS |
| TTGGTTAGTGCATTAGAGAA+TGG | 0.450983 | 5.3:-79427598 | MS.gene38152:CDS |
| GCTGGCTATGCCTTGGTGCA+TGG | 0.452799 | 5.3:-79428185 | MS.gene38152:CDS |
| GATATCTGATTCTTTCAATA+CGG | 0.457053 | 5.3:+79429324 | None:intergenic |
| GATAGAGTCAGGAGAAAAGT+TGG | 0.459646 | 5.3:-79429169 | MS.gene38152:CDS |
| TCAGTAATGTTAATGAAGCT+TGG | 0.462918 | 5.3:-79429204 | MS.gene38152:CDS |
| GAAGCAGTGTGAATCTTAGA+AGG | 0.471251 | 5.3:+79429070 | None:intergenic |
| AATACGGCAAAATTTATCTC+GGG | 0.476016 | 5.3:+79429340 | None:intergenic |
| GACACTGTGCCACAATCCAC+TGG | 0.478077 | 5.3:+79428357 | None:intergenic |
| AATCGTTGAATTCTTTAGAC+GGG | 0.490197 | 5.3:+79427704 | None:intergenic |
| TATTGAAAGAATCAGATATC+CGG | 0.498546 | 5.3:-79429321 | MS.gene38152:CDS |
| ATCAGATATCCGGAAAAGGC+AGG | 0.502816 | 5.3:-79429311 | MS.gene38152:CDS |
| GCTTGCAGCAACTGGTGGTA+TGG | 0.503549 | 5.3:+79429255 | None:intergenic |
| TCAGACCATGGTAGCAGGAC+AGG | 0.504446 | 5.3:-79428162 | MS.gene38152:CDS |
| TTGAAGTGGACATATGCATA+TGG | 0.505690 | 5.3:-79427859 | MS.gene38152:CDS |
| ATGTCATATGCATGCACCCT+AGG | 0.505969 | 5.3:+79428235 | None:intergenic |
| GGGCTAGCAACCAATCTTCA+AGG | 0.506634 | 5.3:-79428017 | MS.gene38152:CDS |
| TTAAGTTCATAACCGAGGCT+TGG | 0.509435 | 5.3:-79427909 | MS.gene38152:CDS |
| TCAATCGGTCTCTTACACTT+GGG | 0.509904 | 5.3:+79428264 | None:intergenic |
| AACCGCCTGTCCTGCTACCA+TGG | 0.512048 | 5.3:+79428157 | None:intergenic |
| GATAATGATTACTTTGATGA+TGG | 0.516035 | 5.3:-79429385 | MS.gene38152:CDS |
| TCCAAATAGTTGAGTGCAGG+CGG | 0.516702 | 5.3:-79427885 | MS.gene38152:CDS |
| AAATGTTGCAGGGAGCAGTA+AGG | 0.521161 | 5.3:-79427520 | None:intergenic |
| ACTTAAGCTCTGCTTCAGCT+TGG | 0.521950 | 5.3:+79427926 | None:intergenic |
| AACTATTTGGAACCAAGCCT+CGG | 0.525306 | 5.3:+79427897 | None:intergenic |
| CATGGTCAGACCATGGTAGC+AGG | 0.527078 | 5.3:-79428167 | MS.gene38152:CDS |
| TTGCTGACGAAGATAGAGTC+AGG | 0.530108 | 5.3:-79429180 | MS.gene38152:CDS |
| CTTTGAGTACTTGCAGGGTG+AGG | 0.532052 | 5.3:-79427797 | MS.gene38152:CDS |
| GGTTCCAAATAGTTGAGTGC+AGG | 0.533830 | 5.3:-79427888 | MS.gene38152:CDS |
| AAGAATCAGATATCCGGAAA+AGG | 0.538730 | 5.3:-79429315 | MS.gene38152:CDS |
| ATCAACAGCAGCACCACAAG+CGG | 0.544045 | 5.3:+79428952 | None:intergenic |
| GTGCATATCATCAGAATCCA+TGG | 0.553746 | 5.3:+79429543 | None:intergenic |
| TCCAATTATAATGACGAAGT+AGG | 0.562132 | 5.3:+79429230 | None:intergenic |
| AAATCGTTGAATTCTTTAGA+CGG | 0.563324 | 5.3:+79427703 | None:intergenic |
| GCATGTTCACAACCAGGAGC+AGG | 0.569609 | 5.3:+79428471 | None:intergenic |
| ACTTCAATTAACGATGGTCC+GGG | 0.571072 | 5.3:-79429001 | MS.gene38152:CDS |
| ACCAGGAGCAGGACACCACT+TGG | 0.572721 | 5.3:+79428482 | None:intergenic |
| CAGCTCTTTGAGTACTTGCA+GGG | 0.579891 | 5.3:-79427802 | MS.gene38152:CDS |
| CCAAATAGTTGAGTGCAGGC+GGG | 0.581218 | 5.3:-79427884 | MS.gene38152:CDS |
| AATGTTGCAGGGAGCAGTAA+GGG | 0.581498 | 5.3:-79427519 | None:intergenic |
| ACACACATTATTATGAGCGG+TGG | 0.585129 | 5.3:-79428038 | MS.gene38152:CDS |
| AGAGAATGGTTTATGTGATG+TGG | 0.585426 | 5.3:-79427584 | MS.gene38152:CDS |
| AAGATTCACACTGCTTCTTG+CGG | 0.591226 | 5.3:-79429064 | MS.gene38152:CDS |
| AGTGCAGGCGGGTATTGAAG+TGG | 0.592470 | 5.3:-79427873 | MS.gene38152:CDS |
| CAAAATTTATCTCGGGAGCA+AGG | 0.594934 | 5.3:+79429347 | None:intergenic |
| TGATCTACACCAGATGCAAA+CGG | 0.597629 | 5.3:-79427980 | MS.gene38152:CDS |
| CATCTCAACATCAAGCATCC+CGG | 0.599330 | 5.3:+79428983 | None:intergenic |
| GGTCTCTTACACTTGGGACA+CGG | 0.599781 | 5.3:+79428270 | None:intergenic |
| GGCAGGAGGATGATATCAGT+AGG | 0.602221 | 5.3:-79429294 | MS.gene38152:CDS |
| GATGACGACGACGATGATGA+TGG | 0.602897 | 5.3:-79429430 | MS.gene38152:CDS |
| TTATGTGATGTGGATAGTAA+TGG | 0.606906 | 5.3:-79427574 | MS.gene38152:CDS |
| GCACACCGTCCAGTGGATTG+TGG | 0.607983 | 5.3:-79428366 | MS.gene38152:CDS |
| AGATGTCCTGATCCCGCTTG+TGG | 0.614660 | 5.3:-79428965 | MS.gene38152:CDS |
| TCAACAGCAGCACCACAAGC+GGG | 0.617382 | 5.3:+79428953 | None:intergenic |
| CACACATTATTATGAGCGGT+GGG | 0.617387 | 5.3:-79428037 | MS.gene38152:CDS |
| CAGCAAAATTCAAAATTGCA+AGG | 0.618074 | 5.3:+79428204 | None:intergenic |
| TGAAGATGCACACCGTCCAG+TGG | 0.618809 | 5.3:-79428373 | MS.gene38152:CDS |
| TCCCTCGATGACGATTTCTA+CGG | 0.622027 | 5.3:-79429502 | MS.gene38152:CDS |
| ACCGTAGAAATCGTCATCGA+GGG | 0.623981 | 5.3:+79429501 | None:intergenic |
| CGTAACATCCAATAAGGTAA+CGG | 0.624303 | 5.3:+79428879 | None:intergenic |
| TGGCAAAGAATTCATTGGAG+AGG | 0.643713 | 5.3:-79428062 | MS.gene38152:CDS |
| GCAGGAGGATGATATCAGTA+GGG | 0.649047 | 5.3:-79429293 | MS.gene38152:CDS |
| CTGTGCCACAATCCACTGGA+CGG | 0.652633 | 5.3:+79428361 | None:intergenic |
| AGGATGCTTGCAGCAACTGG+TGG | 0.656384 | 5.3:+79429250 | None:intergenic |
| CTCGATGACGATTTCTACGG+TGG | 0.658535 | 5.3:-79429499 | MS.gene38152:CDS |
| AGCAAAAGCTATATGAACAG+AGG | 0.658619 | 5.3:+79428406 | None:intergenic |
| CACCGTAGAAATCGTCATCG+AGG | 0.661533 | 5.3:+79429500 | None:intergenic |
| TAGACGGGTCTTCAGTACTG+AGG | 0.664376 | 5.3:+79427719 | None:intergenic |
| GACCATGGTAGCAGGACAGG+CGG | 0.664885 | 5.3:-79428159 | MS.gene38152:CDS |
| ATTGGCACTTCAATTAACGA+TGG | 0.666421 | 5.3:-79429007 | MS.gene38152:CDS |
| AGAGCTTAAGTTCATAACCG+AGG | 0.673682 | 5.3:-79427914 | MS.gene38152:CDS |
| ATTGTGGCACAGTGTCGAAG+TGG | 0.679812 | 5.3:-79428350 | MS.gene38152:CDS |
| AGATATCCGGAAAAGGCAGG+AGG | 0.690418 | 5.3:-79429308 | MS.gene38152:CDS |
| CGAGAGACTTCATCAATGCG+CGG | 0.690979 | 5.3:-79427761 | MS.gene38152:CDS |
| TTAACTGCATGTTCACAACC+AGG | 0.709158 | 5.3:+79428465 | None:intergenic |
| CTTGGTGCATGGTCAGACCA+TGG | 0.709540 | 5.3:-79428174 | MS.gene38152:CDS |
| CATGGTCTGACCATGCACCA+AGG | 0.709916 | 5.3:+79428175 | None:intergenic |
| GGTACACACATTATTATGAG+CGG | 0.716244 | 5.3:-79428041 | MS.gene38152:CDS |
| CAAAATTCAAAATTGCAAGG+TGG | 0.719196 | 5.3:+79428207 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAAAAAAGAAGAGAAA+TGG | - | chr5.3:79428981-79429000 | MS.gene38152:CDS | 20.0% |
| !! | TAAAAACTTCTTCAAGAATT+TGG | - | chr5.3:79429446-79429465 | MS.gene38152:CDS | 20.0% |
| ! | AAATCGTTGAATTCTTTAGA+CGG | + | chr5.3:79429363-79429382 | None:intergenic | 25.0% |
| ! | GAGTTTAAAAAAATGTTGCA+GGG | - | chr5.3:79429533-79429552 | MS.gene38152:CDS | 25.0% |
| ! | GATAATGATTACTTTGATGA+TGG | - | chr5.3:79427678-79427697 | MS.gene38152:CDS | 25.0% |
| ! | GATATCTGATTCTTTCAATA+CGG | + | chr5.3:79427742-79427761 | None:intergenic | 25.0% |
| ! | TATTGAAAGAATCAGATATC+CGG | - | chr5.3:79427742-79427761 | MS.gene38152:CDS | 25.0% |
| ! | TGACTAAAATAAAAGAGAGT+TGG | + | chr5.3:79428432-79428451 | None:intergenic | 25.0% |
| ! | TTACGTTGAAGACAATAAAA+AGG | - | chr5.3:79428202-79428221 | MS.gene38152:CDS | 25.0% |
| !! | ATGAAGACTCTAAAAAAGAT+GGG | - | chr5.3:79428947-79428966 | MS.gene38152:CDS | 25.0% |
| !! | TATGAAGACTCTAAAAAAGA+TGG | - | chr5.3:79428946-79428965 | MS.gene38152:CDS | 25.0% |
| AATACGGCAAAATTTATCTC+GGG | + | chr5.3:79427726-79427745 | None:intergenic | 30.0% | |
| AATCGTTGAATTCTTTAGAC+GGG | + | chr5.3:79429362-79429381 | None:intergenic | 30.0% | |
| AATGCTAAAAAGCTTACTTG+CGG | - | chr5.3:79427948-79427967 | MS.gene38152:CDS | 30.0% | |
| AGAAATGGCAAAGAATTCAT+TGG | - | chr5.3:79428996-79429015 | MS.gene38152:CDS | 30.0% | |
| CAAAATTCAAAATTGCAAGG+TGG | + | chr5.3:79428859-79428878 | None:intergenic | 30.0% | |
| CAATACGGCAAAATTTATCT+CGG | + | chr5.3:79427727-79427746 | None:intergenic | 30.0% | |
| CAGAAGAAATAAGATCGTTA+TGG | + | chr5.3:79428541-79428560 | None:intergenic | 30.0% | |
| CAGCAAAATTCAAAATTGCA+AGG | + | chr5.3:79428862-79428881 | None:intergenic | 30.0% | |
| CGAGTTTAAAAAAATGTTGC+AGG | - | chr5.3:79429532-79429551 | MS.gene38152:CDS | 30.0% | |
| TCAGTAATGTTAATGAAGCT+TGG | - | chr5.3:79427859-79427878 | MS.gene38152:CDS | 30.0% | |
| TCCAATTATAATGACGAAGT+AGG | + | chr5.3:79427836-79427855 | None:intergenic | 30.0% | |
| TCCTACTTCGTCATTATAAT+TGG | - | chr5.3:79427832-79427851 | MS.gene38152:CDS | 30.0% | |
| TGGATTGTATAACAACAAGA+GGG | + | chr5.3:79428521-79428540 | None:intergenic | 30.0% | |
| TTATGTGATGTGGATAGTAA+TGG | - | chr5.3:79429489-79429508 | MS.gene38152:CDS | 30.0% | |
| ! | AAGTATCCAGTTTGTGTTTT+CGG | + | chr5.3:79428761-79428780 | None:intergenic | 30.0% |
| ! | ATGGATTGTATAACAACAAG+AGG | + | chr5.3:79428522-79428541 | None:intergenic | 30.0% |
| ! | CTTGCAATTTTGAATTTTGC+TGG | - | chr5.3:79428860-79428879 | MS.gene38152:intron | 30.0% |
| ! | TGAAGACTCTAAAAAAGATG+GGG | - | chr5.3:79428948-79428967 | MS.gene38152:CDS | 30.0% |
| !! | CAAGTAAGCTTTTTAGCATT+AGG | + | chr5.3:79427948-79427967 | None:intergenic | 30.0% |
| !!! | TTAGTCATTGCTATTTTGCA+TGG | - | chr5.3:79428445-79428464 | MS.gene38152:CDS | 30.0% |
| !!! | TTTGTTTTGTTTAGACCAAG+TGG | - | chr5.3:79428566-79428585 | MS.gene38152:intron | 30.0% |
| AAGAATCAGATATCCGGAAA+AGG | - | chr5.3:79427748-79427767 | MS.gene38152:CDS | 35.0% | |
| AAGTATGACCGTTACCTTAT+TGG | - | chr5.3:79428176-79428195 | MS.gene38152:CDS | 35.0% | |
| ACGAATAATTCTTTGCGTGT+TGG | - | chr5.3:79428312-79428331 | MS.gene38152:CDS | 35.0% | |
| AGACCGATTGAAAAAAACCT+AGG | - | chr5.3:79428811-79428830 | MS.gene38152:intron | 35.0% | |
| AGAGAATGGTTTATGTGATG+TGG | - | chr5.3:79429479-79429498 | MS.gene38152:CDS | 35.0% | |
| AGCAAAAGCTATATGAACAG+AGG | + | chr5.3:79428660-79428679 | None:intergenic | 35.0% | |
| ATCAGAATCCATGCAAATAG+AGG | + | chr5.3:79428494-79428513 | None:intergenic | 35.0% | |
| ATTGGCACTTCAATTAACGA+TGG | - | chr5.3:79428056-79428075 | MS.gene38152:CDS | 35.0% | |
| ATTTGCCCTAATTTATCACC+TGG | - | chr5.3:79428344-79428363 | MS.gene38152:CDS | 35.0% | |
| CGTAACATCCAATAAGGTAA+CGG | + | chr5.3:79428187-79428206 | None:intergenic | 35.0% | |
| CTTCAACGTAACATCCAATA+AGG | + | chr5.3:79428193-79428212 | None:intergenic | 35.0% | |
| GACCGATTGAAAAAAACCTA+GGG | - | chr5.3:79428812-79428831 | MS.gene38152:intron | 35.0% | |
| GCATTAGGATTCTCATAAAC+TGG | + | chr5.3:79427933-79427952 | None:intergenic | 35.0% | |
| GGTACACACATTATTATGAG+CGG | - | chr5.3:79429022-79429041 | MS.gene38152:CDS | 35.0% | |
| GTCATAGTTGTAGTCATCTT+CGG | + | chr5.3:79427595-79427614 | None:intergenic | 35.0% | |
| TATCTTTGCCTCTATTTGCA+TGG | - | chr5.3:79428483-79428502 | MS.gene38152:CDS | 35.0% | |
| TGTAGCCAGGTGATAAATTA+GGG | + | chr5.3:79428352-79428371 | None:intergenic | 35.0% | |
| TTCCAAAATTGTGTGATGTG+TGG | - | chr5.3:79428382-79428401 | MS.gene38152:CDS | 35.0% | |
| TTGAAGTGGACATATGCATA+TGG | - | chr5.3:79429204-79429223 | MS.gene38152:CDS | 35.0% | |
| TTGGTTAGTGCATTAGAGAA+TGG | - | chr5.3:79429465-79429484 | MS.gene38152:CDS | 35.0% | |
| ! | AACCACACATCACACAATTT+TGG | + | chr5.3:79428387-79428406 | None:intergenic | 35.0% |
| ! | ATAATTCTTTGCGTGTTGGT+CGG | - | chr5.3:79428316-79428335 | MS.gene38152:CDS | 35.0% |
| ! | CATGCAGTTAATTTTGTTGC+TGG | - | chr5.3:79428610-79428629 | MS.gene38152:intron | 35.0% |
| ! | TCTGTTCATATAGCTTTTGC+TGG | - | chr5.3:79428659-79428678 | MS.gene38152:intron | 35.0% |
| !! | ATCCATACTGTTTTTCATGC+TGG | - | chr5.3:79428024-79428043 | MS.gene38152:CDS | 35.0% |
| !! | TCCATACTGTTTTTCATGCT+GGG | - | chr5.3:79428025-79428044 | MS.gene38152:CDS | 35.0% |
| !!! | CACCCTAGGTTTTTTTCAAT+CGG | + | chr5.3:79428817-79428836 | None:intergenic | 35.0% |
| !!! | GAAGAAGTTTTTAGTCACAC+TGG | + | chr5.3:79429439-79429458 | None:intergenic | 35.0% |
| AAGATTCACACTGCTTCTTG+CGG | - | chr5.3:79427999-79428018 | MS.gene38152:CDS | 40.0% | |
| ACACACATTATTATGAGCGG+TGG | - | chr5.3:79429025-79429044 | MS.gene38152:CDS | 40.0% | |
| ACTTCAATTAACGATGGTCC+GGG | - | chr5.3:79428062-79428081 | MS.gene38152:CDS | 40.0% | |
| AGAGCTTAAGTTCATAACCG+AGG | - | chr5.3:79429149-79429168 | MS.gene38152:CDS | 40.0% | |
| ATTTATCACCTGGCTACACT+TGG | - | chr5.3:79428354-79428373 | MS.gene38152:CDS | 40.0% | |
| CAAAATTTATCTCGGGAGCA+AGG | + | chr5.3:79427719-79427738 | None:intergenic | 40.0% | |
| CACACATTATTATGAGCGGT+GGG | - | chr5.3:79429026-79429045 | MS.gene38152:CDS | 40.0% | |
| CACTTCAATTAACGATGGTC+CGG | - | chr5.3:79428061-79428080 | MS.gene38152:CDS | 40.0% | |
| CAGAATCCGAAAACACAAAC+TGG | - | chr5.3:79428752-79428771 | MS.gene38152:intron | 40.0% | |
| GAAGCAGTGTGAATCTTAGA+AGG | + | chr5.3:79427996-79428015 | None:intergenic | 40.0% | |
| GATAGAGTCAGGAGAAAAGT+TGG | - | chr5.3:79427894-79427913 | MS.gene38152:CDS | 40.0% | |
| GTGTAGCCAGGTGATAAATT+AGG | + | chr5.3:79428353-79428372 | None:intergenic | 40.0% | |
| TCAATCGGTCTCTTACACTT+GGG | + | chr5.3:79428802-79428821 | None:intergenic | 40.0% | |
| TGATCTACACCAGATGCAAA+CGG | - | chr5.3:79429083-79429102 | MS.gene38152:CDS | 40.0% | |
| TGGCAAAGAATTCATTGGAG+AGG | - | chr5.3:79429001-79429020 | MS.gene38152:CDS | 40.0% | |
| TTAACTGCATGTTCACAACC+AGG | + | chr5.3:79428601-79428620 | None:intergenic | 40.0% | |
| TTAAGTTCATAACCGAGGCT+TGG | - | chr5.3:79429154-79429173 | MS.gene38152:CDS | 40.0% | |
| TTCAATCGGTCTCTTACACT+TGG | + | chr5.3:79428803-79428822 | None:intergenic | 40.0% | |
| TTTATCACCTGGCTACACTT+GGG | - | chr5.3:79428355-79428374 | MS.gene38152:CDS | 40.0% | |
| ! | AACTATTTGGAACCAAGCCT+CGG | + | chr5.3:79429169-79429188 | None:intergenic | 40.0% |
| ! | CTTCAGTACTGAGGAATACT+TGG | + | chr5.3:79429338-79429357 | None:intergenic | 40.0% |
| ! | GAGCTTTTTGCCTTGAAGAT+TGG | + | chr5.3:79429059-79429078 | None:intergenic | 40.0% |
| ! | GATTTTCGCTCAAAACTAGC+TGG | - | chr5.3:79429408-79429427 | MS.gene38152:CDS | 40.0% |
| ! | GCAGTTAATTTTGTTGCTGG+TGG | - | chr5.3:79428613-79428632 | MS.gene38152:intron | 40.0% |
| !! | CCATACTGTTTTTCATGCTG+GGG | - | chr5.3:79428026-79428045 | MS.gene38152:CDS | 40.0% |
| ACCGTAGAAATCGTCATCGA+GGG | + | chr5.3:79427565-79427584 | None:intergenic | 45.0% | |
| ACTTAAGCTCTGCTTCAGCT+TGG | + | chr5.3:79429140-79429159 | None:intergenic | 45.0% | |
| ACTTCATCAATGCGCGGAAA+AGG | - | chr5.3:79429308-79429327 | MS.gene38152:CDS | 45.0% | |
| ATCAGATATCCGGAAAAGGC+AGG | - | chr5.3:79427752-79427771 | MS.gene38152:CDS | 45.0% | |
| ATGTCATATGCATGCACCCT+AGG | + | chr5.3:79428831-79428850 | None:intergenic | 45.0% | |
| CAGCTCTTTGAGTACTTGCA+GGG | - | chr5.3:79429261-79429280 | MS.gene38152:CDS | 45.0% | |
| CATCTCAACATCAAGCATCC+CGG | + | chr5.3:79428083-79428102 | None:intergenic | 45.0% | |
| CCAGATGCAAACGGTTCATA+TGG | - | chr5.3:79429092-79429111 | MS.gene38152:CDS | 45.0% | |
| CCATATGAACCGTTTGCATC+TGG | + | chr5.3:79429095-79429114 | None:intergenic | 45.0% | |
| CCCCAGCATGAAAAACAGTA+TGG | + | chr5.3:79428029-79428048 | None:intergenic | 45.0% | |
| GCAGGAGGATGATATCAGTA+GGG | - | chr5.3:79427770-79427789 | MS.gene38152:CDS | 45.0% | |
| GGTTCCAAATAGTTGAGTGC+AGG | - | chr5.3:79429175-79429194 | MS.gene38152:CDS | 45.0% | |
| TCATGCTGGGGAGGATATAT+TGG | - | chr5.3:79428038-79428057 | MS.gene38152:CDS | 45.0% | |
| TCCAAATAGTTGAGTGCAGG+CGG | - | chr5.3:79429178-79429197 | MS.gene38152:CDS | 45.0% | |
| TCCCTCGATGACGATTTCTA+CGG | - | chr5.3:79427561-79427580 | MS.gene38152:CDS | 45.0% | |
| TTGCTGACGAAGATAGAGTC+AGG | - | chr5.3:79427883-79427902 | MS.gene38152:CDS | 45.0% | |
| ! | TATCATCCTCCTGCCTTTTC+CGG | + | chr5.3:79427764-79427783 | None:intergenic | 45.0% |
| !! | CAGGAGAAAAGTTGGCTTGT+TGG | - | chr5.3:79427902-79427921 | MS.gene38152:CDS | 45.0% |
| !! | GAATTTTGCTGGCTATGCCT+TGG | - | chr5.3:79428871-79428890 | MS.gene38152:CDS | 45.0% |
| !! | TACTGTTTTTCATGCTGGGG+AGG | - | chr5.3:79428029-79428048 | MS.gene38152:CDS | 45.0% |
| AGATATCCGGAAAAGGCAGG+AGG | - | chr5.3:79427755-79427774 | MS.gene38152:CDS | 50.0% | |
| AGTAGGATGCTTGCAGCAAC+TGG | + | chr5.3:79427819-79427838 | None:intergenic | 50.0% | |
| ATCAACAGCAGCACCACAAG+CGG | + | chr5.3:79428114-79428133 | None:intergenic | 50.0% | |
| CACCGTAGAAATCGTCATCG+AGG | + | chr5.3:79427566-79427585 | None:intergenic | 50.0% | |
| CCAAATAGTTGAGTGCAGGC+GGG | - | chr5.3:79429179-79429198 | MS.gene38152:CDS | 50.0% | |
| CGAGAGACTTCATCAATGCG+CGG | - | chr5.3:79429302-79429321 | MS.gene38152:CDS | 50.0% | |
| CTCGATGACGATTTCTACGG+TGG | - | chr5.3:79427564-79427583 | MS.gene38152:CDS | 50.0% | |
| CTTTGAGTACTTGCAGGGTG+AGG | - | chr5.3:79429266-79429285 | MS.gene38152:CDS | 50.0% | |
| GAATTCACCCAAGTGTAGCC+AGG | + | chr5.3:79428365-79428384 | None:intergenic | 50.0% | |
| GATGACGACGACGATGATGA+TGG | - | chr5.3:79427633-79427652 | MS.gene38152:CDS | 50.0% | |
| GCAGCTCTTTGAGTACTTGC+AGG | - | chr5.3:79429260-79429279 | MS.gene38152:CDS | 50.0% | |
| GGCAGGAGGATGATATCAGT+AGG | - | chr5.3:79427769-79427788 | MS.gene38152:CDS | 50.0% | |
| GGTCTCTTACACTTGGGACA+CGG | + | chr5.3:79428796-79428815 | None:intergenic | 50.0% | |
| TAGACGGGTCTTCAGTACTG+AGG | + | chr5.3:79429347-79429366 | None:intergenic | 50.0% | |
| ! | ATTGTGGCACAGTGTCGAAG+TGG | - | chr5.3:79428713-79428732 | MS.gene38152:intron | 50.0% |
| ! | GGGCTAGCAACCAATCTTCA+AGG | - | chr5.3:79429046-79429065 | MS.gene38152:CDS | 50.0% |
| AGATGTCCTGATCCCGCTTG+TGG | - | chr5.3:79428098-79428117 | MS.gene38152:CDS | 55.0% | |
| AGGATGCTTGCAGCAACTGG+TGG | + | chr5.3:79427816-79427835 | None:intergenic | 55.0% | |
| CATGGTCAGACCATGGTAGC+AGG | - | chr5.3:79428896-79428915 | MS.gene38152:CDS | 55.0% | |
| CATGGTCTGACCATGCACCA+AGG | + | chr5.3:79428891-79428910 | None:intergenic | 55.0% | |
| CCCGCCTGCACTCAACTATT+TGG | + | chr5.3:79429182-79429201 | None:intergenic | 55.0% | |
| CTGTGCCACAATCCACTGGA+CGG | + | chr5.3:79428705-79428724 | None:intergenic | 55.0% | |
| GACACTGTGCCACAATCCAC+TGG | + | chr5.3:79428709-79428728 | None:intergenic | 55.0% | |
| GCATGTTCACAACCAGGAGC+AGG | + | chr5.3:79428595-79428614 | None:intergenic | 55.0% | |
| GCTTGCAGCAACTGGTGGTA+TGG | + | chr5.3:79427811-79427830 | None:intergenic | 55.0% | |
| TCAACAGCAGCACCACAAGC+GGG | + | chr5.3:79428113-79428132 | None:intergenic | 55.0% | |
| TCAGACCATGGTAGCAGGAC+AGG | - | chr5.3:79428901-79428920 | MS.gene38152:CDS | 55.0% | |
| TGAAGATGCACACCGTCCAG+TGG | - | chr5.3:79428690-79428709 | MS.gene38152:intron | 55.0% | |
| ! | CTTGGTGCATGGTCAGACCA+TGG | - | chr5.3:79428889-79428908 | MS.gene38152:CDS | 55.0% |
| !! | AGTGCAGGCGGGTATTGAAG+TGG | - | chr5.3:79429190-79429209 | MS.gene38152:CDS | 55.0% |
| AACCGCCTGTCCTGCTACCA+TGG | + | chr5.3:79428909-79428928 | None:intergenic | 60.0% | |
| ACCAAGTGGTGTCCTGCTCC+TGG | - | chr5.3:79428580-79428599 | MS.gene38152:intron | 60.0% | |
| ACCAGGAGCAGGACACCACT+TGG | + | chr5.3:79428584-79428603 | None:intergenic | 60.0% | |
| GACCATGGTAGCAGGACAGG+CGG | - | chr5.3:79428904-79428923 | MS.gene38152:CDS | 60.0% | |
| ! | GCTGGCTATGCCTTGGTGCA+TGG | - | chr5.3:79428878-79428897 | MS.gene38152:CDS | 60.0% |
| !! | GCACACCGTCCAGTGGATTG+TGG | - | chr5.3:79428697-79428716 | MS.gene38152:intron | 60.0% |
| GCAGCACCACAAGCGGGATC+AGG | + | chr5.3:79428107-79428126 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 79427522 | 79429563 | 79427522 | ID=MS.gene38152 |
| chr5.3 | mRNA | 79427522 | 79429563 | 79427522 | ID=MS.gene38152.t1;Parent=MS.gene38152 |
| chr5.3 | exon | 79428862 | 79429563 | 79428862 | ID=MS.gene38152.t1.exon1;Parent=MS.gene38152.t1 |
| chr5.3 | CDS | 79428862 | 79429563 | 79428862 | ID=cds.MS.gene38152.t1;Parent=MS.gene38152.t1 |
| chr5.3 | exon | 79427522 | 79428505 | 79427522 | ID=MS.gene38152.t1.exon2;Parent=MS.gene38152.t1 |
| chr5.3 | CDS | 79427522 | 79428505 | 79427522 | ID=cds.MS.gene38152.t1;Parent=MS.gene38152.t1 |
| Gene Sequence |
| Protein sequence |
