AlfalfaGEDB | MS.gene39856

M. sativa cultivar XinJiangDaYe / MS.gene39856

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39856.t1	RHN60307.1	70.1	284	51	2	80	339	787	1060	1.70E-72	283.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39856.t1	Q5MR23	37.5	104	57	4	234	330	760	862	7.1e-11	69.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39856.t1	A0A072UKQ2	70.1	284	51	2	80	339	787	1060	1.2e-72	283.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049222	MS.gene39856	0.804364	2.24E-49	-1.69E-46
MS.gene049609	MS.gene39856	0.836011	1.20E-56	-1.69E-46
MS.gene050170	MS.gene39856	0.841609	4.28E-58	-1.69E-46
MS.gene050675	MS.gene39856	0.808774	2.63E-50	-1.69E-46
MS.gene050747	MS.gene39856	0.830008	3.74E-55	-1.69E-46
MS.gene050772	MS.gene39856	0.819751	9.85E-53	-1.69E-46
MS.gene050950	MS.gene39856	0.821935	3.10E-53	-1.69E-46
MS.gene051438	MS.gene39856	0.807404	5.14E-50	-1.69E-46
MS.gene051838	MS.gene39856	0.815907	7.27E-52	-1.69E-46
MS.gene051941	MS.gene39856	0.824809	6.60E-54	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene37823	MS.gene39856	PPI
MS.gene39856	MS.gene99384	PPI
MS.gene39856	MS.gene39814	PPI
MS.gene39856	MS.gene016865	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39856.t1	MTR_5g087320	85.269	353	38	1	1	339	668	1020	0.0	615
MS.gene39856.t1	MTR_5g087320	30.859	256	157	8	2	246	379	625	1.12e-16	81.6
MS.gene39856.t1	MTR_5g087320	29.474	285	149	10	12	259	508	777	2.18e-13	71.6
MS.gene39856.t1	MTR_5g087320	29.130	230	104	5	4	231	262	434	2.21e-12	68.6
MS.gene39856.t1	MTR_4g046920	85.552	353	37	1	1	339	708	1060	0.0	613
MS.gene39856.t1	MTR_4g046920	28.523	298	154	9	2	247	376	666	6.14e-13	70.5
MS.gene39856.t1	MTR_4g046920	27.586	261	128	9	41	246	557	811	3.48e-11	65.1
MS.gene39856.t1	MTR_5g086810	84.703	353	40	1	1	339	669	1021	0.0	599
MS.gene39856.t1	MTR_5g086810	28.671	286	141	9	2	232	377	654	8.76e-18	85.1
MS.gene39856.t1	MTR_5g086810	29.536	237	134	5	6	229	309	525	1.08e-13	72.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39856.t1	AT3G05660	44.308	325	156	6	2	310	538	853	2.11e-74	246
MS.gene39856.t1	AT3G05660	29.614	233	123	6	20	250	115	308	6.43e-11	63.9
MS.gene39856.t1	AT2G33060	42.183	339	162	8	2	316	444	772	2.28e-71	237
MS.gene39856.t1	AT2G33060	29.557	203	128	5	33	231	308	499	6.31e-12	67.0
MS.gene39856.t1	AT2G15042	42.679	321	152	7	2	300	88	398	2.28e-70	225
MS.gene39856.t1	AT2G33020	38.953	344	175	7	2	316	525	862	3.95e-67	226
MS.gene39856.t1	AT2G33020	29.108	213	114	4	32	231	392	580	2.39e-14	74.7
MS.gene39856.t1	AT2G33020	28.063	253	143	8	1	236	216	446	1.11e-11	66.6
MS.gene39856.t1	AT5G25910	41.399	343	166	8	2	319	476	808	1.58e-66	224
MS.gene39856.t1	AT5G25910	35.021	237	127	6	2	232	269	484	1.25e-22	99.4

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Find 74 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTAGCACCTGTAACTC+TGG	0.497218	5.4:+68611254	None:intergenic
AAAGTTGAATGGCAACCAAT+TGG	0.442634	5.4:-68611381	MS.gene39856:CDS
AAATGCATTTCCTTTGGAAA+AGG	0.422403	5.4:+68611412	None:intergenic
AAGAAGAATCGGGATTTGGC+TGG	0.490064	5.4:-68610582	MS.gene39856:CDS
AAGTTCTTGATGCATGATGT+TGG	0.637838	5.4:+68611125	None:intergenic
AATGAGAATGACAGCCAGAG+TGG	0.645850	5.4:-68611089	MS.gene39856:CDS
ACATGTCTTGCAAGGCATTG+AGG	0.542018	5.4:+68610478	None:intergenic
AGAAACTCTGCCAGAGTTAC+AGG	0.293514	5.4:-68611264	MS.gene39856:CDS
AGCAATTGGATATGCATGTG+GGG	0.730826	5.4:-68610551	MS.gene39856:CDS
AGGACCATTACCACAATCTT+TGG	0.325353	5.4:-68611357	MS.gene39856:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTTTCAAATGCATTTCCTT+TGG	+	chr5.4:68610445-68610464	None:intergenic	30.0%
ATCTGAAGATGAAGAAGAAT+CGG	-	chr5.4:68611255-68611274	MS.gene39856:CDS	30.0%
CCTTTCAAAAAATGGAATCA+CGG	-	chr5.4:68610961-68610980	MS.gene39856:CDS	30.0%
CTTTCAAAAAATGGAATCAC+GGG	-	chr5.4:68610962-68610981	MS.gene39856:CDS	30.0%
GGACTTAACCTTTCAAAAAA+TGG	-	chr5.4:68610953-68610972	MS.gene39856:intron	30.0%
GTCTAAATCATGCAAAAATG+AGG	-	chr5.4:68611210-68611229	MS.gene39856:CDS	30.0%
TAACATTGTATTCCCTTCAT+AGG	+	chr5.4:68611176-68611195	None:intergenic	30.0%
TTGGAAATGATTCCTATGAA+GGG	-	chr5.4:68611161-68611180	MS.gene39856:CDS	30.0%
TTTGGAAATGATTCCTATGA+AGG	-	chr5.4:68611160-68611179	MS.gene39856:CDS	30.0%
AAAGTTGAATGGCAACCAAT+TGG	-	chr5.4:68610467-68610486	MS.gene39856:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	68610405	68611465	68610405	ID=MS.gene39856
chr5.4	mRNA	68610405	68611465	68610405	ID=MS.gene39856.t1;Parent=MS.gene39856
chr5.4	exon	68610958	68611465	68610958	ID=MS.gene39856.t1.exon1;Parent=MS.gene39856.t1
chr5.4	CDS	68610958	68611465	68610958	ID=cds.MS.gene39856.t1;Parent=MS.gene39856.t1
chr5.4	exon	68610405	68610916	68610405	ID=MS.gene39856.t1.exon2;Parent=MS.gene39856.t1
chr5.4	CDS	68610405	68610916	68610405	ID=cds.MS.gene39856.t1;Parent=MS.gene39856.t1

Gene Sequence

>MS.gene39856

ATGAACAGCCTCTATGGAAGCATTCCTAAAACCTTTTCCAAAGGAAATGCATTTGAAACTATAAAGTTGAATGGCAACCAATTGGAAGGACCATTACCACAATCTTTGGCTCACTGTTCAAATCTTGAAGTTTTGGACCTTGGTGATAACAACATAGAGGATACATTTCCCAACTGGCTAGAAACTCTGCCAGAGTTACAGGTGCTAAGTTTACGATCAAATAATCTTCATGGTGCAATCACTTGTTCTAGCACCAAGCATTCATTTCCTAAGTTGAGAATTTTTGATGTCTCTAACAACAATTTTAGTGGCCCCTTGCCAACATCATGCATCAAGAACTTTCAAGGAATGATGAATGAGAATGACAGCCAGAGTGGTTTGCAATACATGGGTACAACCGATTACTATAATGATTCTTTGGTGGTCATAATGAAAGGTGTTTTCATGGAGCTAACAAGGATATTGACTACTTTCACAACCATTGATTTATCAAATAACATGTTTGAAGGTGAAATTCCACAGTCATTGGAGAGTTATATTCTCTCATAGGACTTAACCTTTCAAAAAATGGAATCACGGGTAGCATTCCACAATCTTTGAGTCATTTGAGAAATTTGGAGTGGTTGGACCTCTCATGCAACCAATTGACGGGTGAGATTCCTGAGGCTTTGACAAATTTGAACTTCCTCTCTGTCTTAAACCTTTCACAAAACCACCTTAAGGGAATCATACCTAAAGGTCAACAGTTTGATACATTTGGAAATGATTCCTATGAAGGGAATACAATGTTATGTGGATTCCCATTGTCTAAATCATGCAAAAATGAGGAAGATCTGCCACCACATTCAACATCTGAAGATGAAGAAGAATCGGGATTTGGCTGGAAAGCTGTAGCAATTGGATATGCATGTGGGGCAATATTTGAGTTGCTCTTGGGTTATAATGTATTCTTCTTCACTGGAAAACCTCAATGCCTTGCAAGACATGTTGAACGTGTGTTTAACATAAGATTGAAAAGAACAAACACCAGAGCCAGCGCAAATCGCAGAAGAATGAATTAA

Protein sequence

>MS.gene39856.t1

MNSLYGSIPKTFSKGNAFETIKLNGNQLEGPLPQSLAHCSNLEVLDLGDNNIEDTFPNWLETLPELQVLSLRSNNLHGAITCSSTKHSFPKLRIFDVSNNNFSGPLPTSCIKNFQGMMNENDSQSGLQYMGTTDYYNDSLVVIMKGVFMELTRILTTFTTIDLSNNMFEGLNLSKNGITGSIPQSLSHLRNLEWLDLSCNQLTGEIPEALTNLNFLSVLNLSQNHLKGIIPKGQQFDTFGNDSYEGNTMLCGFPLSKSCKNEEDLPPHSTSEDEEESGFGWKAVAIGYACGAIFELLLGYNVFFFTGKPQCLARHVERVFNIRLKRTNTRASANRRRMN