AlfalfaGEDB | MS.gene49839

M. sativa cultivar XinJiangDaYe / MS.gene49839

KEGG: K13754
GO: GO:0005575 GO:0006810 GO:0008150 GO:0016021 GO:0031224 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF01699.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49839.t1	XP_013451524.1	100	206	0	0	1	206	381	586	3.40E-108	401

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49839.t1	Q9FKP1	61.6	198	76	0	9	206	370	567	8.0e-66	251.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49839.t1	A0A072U799	100.0	206	0	0	1	206	381	586	2.4e-108	401.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene49839	0.807803	4.23E-50	-1.69E-46
MS.gene050020	MS.gene49839	0.818469	1.93E-52	-1.69E-46
MS.gene050303	MS.gene49839	0.82842	9.08E-55	-1.69E-46
MS.gene050438	MS.gene49839	0.82096	5.20E-53	-1.69E-46
MS.gene050442	MS.gene49839	0.800117	1.67E-48	-1.69E-46
MS.gene050815	MS.gene49839	0.85083	1.32E-60	-1.69E-46
MS.gene051626	MS.gene49839	0.821355	4.22E-53	-1.69E-46
MS.gene051789	MS.gene49839	0.824745	6.83E-54	-1.69E-46
MS.gene051860	MS.gene49839	0.803867	2.84E-49	-1.69E-46
MS.gene052376	MS.gene49839	0.804522	2.07E-49	-1.69E-46
MS.gene05288	MS.gene49839	0.833771	4.40E-56	-1.69E-46
MS.gene05289	MS.gene49839	0.808479	3.04E-50	-1.69E-46
MS.gene053008	MS.gene49839	0.821806	3.32E-53	-1.69E-46
MS.gene053074	MS.gene49839	0.879884	8.65E-70	-1.69E-46
MS.gene053184	MS.gene49839	0.819026	1.44E-52	-1.69E-46
MS.gene053630	MS.gene49839	0.801391	9.21E-49	-1.69E-46
MS.gene054184	MS.gene49839	0.858783	6.50E-63	-1.69E-46
MS.gene054185	MS.gene49839	0.861032	1.36E-63	-1.69E-46
MS.gene054596	MS.gene49839	0.818434	1.96E-52	-1.69E-46
MS.gene054658	MS.gene49839	0.80103	1.09E-48	-1.69E-46
MS.gene054713	MS.gene49839	0.868839	4.85E-66	-1.69E-46
MS.gene05510	MS.gene49839	0.805256	1.46E-49	-1.69E-46
MS.gene055608	MS.gene49839	0.843632	1.24E-58	-1.69E-46
MS.gene055860	MS.gene49839	0.81882	1.61E-52	-1.69E-46
MS.gene057693	MS.gene49839	0.810146	1.33E-50	-1.69E-46
MS.gene057754	MS.gene49839	0.807335	5.32E-50	-1.69E-46
MS.gene058042	MS.gene49839	0.857508	1.56E-62	-1.69E-46
MS.gene058168	MS.gene49839	0.8579	1.19E-62	-1.69E-46
MS.gene058549	MS.gene49839	0.800101	1.69E-48	-1.69E-46
MS.gene059006	MS.gene49839	0.846312	2.35E-59	-1.69E-46
MS.gene059124	MS.gene49839	0.820497	6.65E-53	-1.69E-46
MS.gene059249	MS.gene49839	0.805162	1.52E-49	-1.69E-46
MS.gene059421	MS.gene49839	0.809031	2.31E-50	-1.69E-46
MS.gene05979	MS.gene49839	0.81152	6.72E-51	-1.69E-46
MS.gene060429	MS.gene49839	0.809672	1.69E-50	-1.69E-46
MS.gene060803	MS.gene49839	0.846616	1.94E-59	-1.69E-46
MS.gene060998	MS.gene49839	0.802172	6.37E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49839.t1	MTR_6g027580	100.000	206	0	0	1	206	381	586	3.96e-143	411
MS.gene49839.t1	MTR_7g086690	58.291	199	83	0	8	206	385	583	9.13e-70	223
MS.gene49839.t1	MTR_6g027570	58.291	199	83	0	8	206	374	572	1.11e-67	217
MS.gene49839.t1	MTR_8g021180	54.187	203	92	1	1	203	445	646	2.44e-64	210
MS.gene49839.t1	MTR_7g113730	38.824	170	101	1	33	202	349	515	4.55e-23	96.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49839.t1	AT5G17860	61.000	200	78	0	7	206	333	532	2.69e-81	251
MS.gene49839.t1	AT5G17860	61.000	200	78	0	7	206	368	567	1.07e-80	251
MS.gene49839.t1	AT5G17850	56.995	193	83	0	11	203	364	556	6.46e-65	209
MS.gene49839.t1	AT5G17850	56.995	193	83	0	11	203	364	556	6.46e-65	209
MS.gene49839.t1	AT1G54115	50.739	203	99	1	1	203	437	638	2.10e-64	210
MS.gene49839.t1	AT3G14070	49.754	203	101	1	1	203	436	637	6.29e-64	209
MS.gene49839.t1	AT1G08960	38.021	192	109	2	10	201	361	542	1.50e-29	114

Find 38 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACCTTTGTTTCTTGTTTATA+AGG	0.167874	6.3:-19214629	MS.gene49839:CDS
TTAGGAACAACGTAAGAATT+AGG	0.188321	6.3:+19214774	None:intergenic
TCCTAAGGATAGTTCACTTT+TGG	0.246796	6.3:-19214757	MS.gene49839:CDS
TCATTGATTGGAATTGTCTT+TGG	0.261248	6.3:-19215149	MS.gene49839:CDS
ATTTCAATTGGATATGTAAT+TGG	0.264822	6.3:-19214996	MS.gene49839:CDS
GGTGCACAAATTGCTGTTTC+AGG	0.282821	6.3:-19214873	MS.gene49839:CDS
CAAGAAGATGTTTGTTTCCT+TGG	0.294111	6.3:-19215088	MS.gene49839:CDS
ACCTCTTGTTCTTTCAGCAT+GGG	0.313872	6.3:-19214805	MS.gene49839:CDS
TATTTAACATCATCATTGAT+TGG	0.352924	6.3:-19215161	MS.gene49839:CDS
CAAGGAAACAAACATCTTCT+TGG	0.367485	6.3:+19215089	None:intergenic
TATGCAACTAAGCTACTCTT+TGG	0.370812	6.3:+19215182	None:intergenic
AAACAAGAAACAAAGGTAAA+TGG	0.374670	6.3:+19214635	None:intergenic
TACCTCTTGTTCTTTCAGCA+TGG	0.389379	6.3:-19214806	MS.gene49839:CDS
TAGGAACAACGTAAGAATTA+GGG	0.421067	6.3:+19214775	None:intergenic
CTAGGTGATTTGATAGCAAA+TGG	0.424425	6.3:-19214924	MS.gene49839:CDS
TGTTTGTTTCCTTGGCTAGC+TGG	0.426986	6.3:-19215080	MS.gene49839:CDS
TAATTCTTACGTTGTTCCTA+AGG	0.451255	6.3:-19214772	MS.gene49839:CDS
ATAAGGTTAGCCATGGCAAT+TGG	0.456604	6.3:-19214612	MS.gene49839:CDS
GCTGTTTCAGGTTGTTATGC+TGG	0.461252	6.3:-19214861	MS.gene49839:CDS
TTGGCTTGGGGAAATTCTCT+AGG	0.480332	6.3:-19214942	MS.gene49839:CDS
ACGGTTAGCCCTAAAATTGA+AGG	0.489309	6.3:+19214966	None:intergenic
CCTCTTGTTCTTTCAGCATG+GGG	0.507419	6.3:-19214804	MS.gene49839:CDS
CCCAAAAGTGAACTATCCTT+AGG	0.527760	6.3:+19214756	None:intergenic
CTTGTTTATAAGGTTAGCCA+TGG	0.529470	6.3:-19214619	MS.gene49839:CDS
AGAATTTCCCCAAGCCAAAA+CGG	0.537248	6.3:+19214947	None:intergenic
CTCTTGTTCTTTCAGCATGG+GGG	0.543298	6.3:-19214803	MS.gene49839:CDS
GGAAACAAACATCTTCTTGG+TGG	0.548039	6.3:+19215092	None:intergenic
TCTTGTTCTTTCAGCATGGG+GGG	0.549335	6.3:-19214802	MS.gene49839:CDS
TTGTTTCCTTGGCTAGCTGG+TGG	0.552371	6.3:-19215077	MS.gene49839:CDS
TGGAATTGTCTTTGGAAACA+TGG	0.555855	6.3:-19215141	MS.gene49839:CDS
CAATACACAAATGGAAAATG+TGG	0.556780	6.3:-19215207	None:intergenic
CCCCATGCTGAAAGAACAAG+AGG	0.576639	6.3:+19214804	None:intergenic
ATTAAGAGCACCAATTGCCA+TGG	0.587028	6.3:+19214602	None:intergenic
TTTGATAGCAAATGGTGCTA+TGG	0.595159	6.3:-19214916	MS.gene49839:CDS
GAAAATCCACCAGCTAGCCA+AGG	0.618132	6.3:+19215071	None:intergenic
CCCATCAAAATATTGAACAT+AGG	0.620623	6.3:+19214837	None:intergenic
ACCTTATAAACAAGAAACAA+AGG	0.640232	6.3:+19214628	None:intergenic
ATGGACTTATATAATAGCTG+AGG	0.679474	6.3:-19215036	MS.gene49839:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTCAATTGGATATGTAAT+TGG	-	chr6.3:19214800-19214819	MS.gene49839:CDS	20.0%
!!	TATTTAACATCATCATTGAT+TGG	-	chr6.3:19214635-19214654	MS.gene49839:CDS	20.0%
!!!	TTTTAGTTAAGAAGAATATG+AGG	-	chr6.3:19215107-19215126	MS.gene49839:CDS	20.0%
!	AAACAAGAAACAAAGGTAAA+TGG	+	chr6.3:19215164-19215183	None:intergenic	25.0%
!	ACCTTATAAACAAGAAACAA+AGG	+	chr6.3:19215171-19215190	None:intergenic	25.0%
!!	ACCTTTGTTTCTTGTTTATA+AGG	-	chr6.3:19215167-19215186	MS.gene49839:CDS	25.0%
!!!	AATATTGTTTTTGATGGTAG+GGG	-	chr6.3:19215066-19215085	MS.gene49839:CDS	25.0%
!!!	ACAATATTGTTTTTGATGGT+AGG	-	chr6.3:19215064-19215083	MS.gene49839:CDS	25.0%
!!!	CAATATTGTTTTTGATGGTA+GGG	-	chr6.3:19215065-19215084	MS.gene49839:CDS	25.0%
!!!	CCTATGTTCAATATTTTGAT+GGG	-	chr6.3:19214959-19214978	MS.gene49839:CDS	25.0%
!!!	GTTTCACTTTTGATTTCAAT+TGG	-	chr6.3:19214788-19214807	MS.gene49839:CDS	25.0%
!!!	TCAATATTTTGATGGGTTTA+GGG	-	chr6.3:19214966-19214985	MS.gene49839:CDS	25.0%
!!!	TCCTATGTTCAATATTTTGA+TGG	-	chr6.3:19214958-19214977	MS.gene49839:CDS	25.0%
!!!	TTCAATATTTTGATGGGTTT+AGG	-	chr6.3:19214965-19214984	MS.gene49839:CDS	25.0%
	ATGGACTTATATAATAGCTG+AGG	-	chr6.3:19214760-19214779	MS.gene49839:CDS	30.0%
	CCCATCAAAATATTGAACAT+AGG	+	chr6.3:19214962-19214981	None:intergenic	30.0%
	TAATTCTTACGTTGTTCCTA+AGG	-	chr6.3:19215024-19215043	MS.gene49839:CDS	30.0%
	TAGGAACAACGTAAGAATTA+GGG	+	chr6.3:19215024-19215043	None:intergenic	30.0%
	TTAGGAACAACGTAAGAATT+AGG	+	chr6.3:19215025-19215044	None:intergenic	30.0%
!	ATGAGGCTTGATAAGTTTTT+AGG	-	chr6.3:19215124-19215143	MS.gene49839:CDS	30.0%
!	GTGTTAGTCCTTCAATTTTA+GGG	-	chr6.3:19214822-19214841	MS.gene49839:CDS	30.0%
!	TCATTGATTGGAATTGTCTT+TGG	-	chr6.3:19214647-19214666	MS.gene49839:CDS	30.0%
	CAAGAAGATGTTTGTTTCCT+TGG	-	chr6.3:19214708-19214727	MS.gene49839:CDS	35.0%
	CAAGGAAACAAACATCTTCT+TGG	+	chr6.3:19214710-19214729	None:intergenic	35.0%
	TATGCAACTAAGCTACTCTT+TGG	+	chr6.3:19214617-19214636	None:intergenic	35.0%
!	CTAGGTGATTTGATAGCAAA+TGG	-	chr6.3:19214872-19214891	MS.gene49839:CDS	35.0%
!	CTTGTTTATAAGGTTAGCCA+TGG	-	chr6.3:19215177-19215196	MS.gene49839:CDS	35.0%
!	GATTTTCAATGAGTGTGACA+TGG	-	chr6.3:19214741-19214760	MS.gene49839:CDS	35.0%
!	GGTGTTAGTCCTTCAATTTT+AGG	-	chr6.3:19214821-19214840	MS.gene49839:CDS	35.0%
!	TCCTAAGGATAGTTCACTTT+TGG	-	chr6.3:19215039-19215058	MS.gene49839:CDS	35.0%
!	TGGAATTGTCTTTGGAAACA+TGG	-	chr6.3:19214655-19214674	MS.gene49839:CDS	35.0%
!	TTTGATAGCAAATGGTGCTA+TGG	-	chr6.3:19214880-19214899	MS.gene49839:CDS	35.0%
!!	AATGGTGCTATGGCTAAAAA+TGG	-	chr6.3:19214890-19214909	MS.gene49839:CDS	35.0%
!!	CCTAAGGATAGTTCACTTTT+GGG	-	chr6.3:19215040-19215059	MS.gene49839:CDS	35.0%
!!	CTAAGGATAGTTCACTTTTG+GGG	-	chr6.3:19215041-19215060	MS.gene49839:CDS	35.0%
!!	CTTGATAAGTTTTTAGGAGC+TGG	-	chr6.3:19215130-19215149	MS.gene49839:CDS	35.0%
!!!	AATTTTAGGGCTAACCGTTT+TGG	-	chr6.3:19214835-19214854	MS.gene49839:CDS	35.0%
!!!	GGGGACAATATTGTTTTTGA+TGG	-	chr6.3:19215060-19215079	MS.gene49839:CDS	35.0%
	ACCTCTTGTTCTTTCAGCAT+GGG	-	chr6.3:19214991-19215010	MS.gene49839:CDS	40.0%
	AGAATTTCCCCAAGCCAAAA+CGG	+	chr6.3:19214852-19214871	None:intergenic	40.0%
	CCCAAAAGTGAACTATCCTT+AGG	+	chr6.3:19215043-19215062	None:intergenic	40.0%
	GGAAACAAACATCTTCTTGG+TGG	+	chr6.3:19214707-19214726	None:intergenic	40.0%
	TACCTCTTGTTCTTTCAGCA+TGG	-	chr6.3:19214990-19215009	MS.gene49839:CDS	40.0%
!	ACGGTTAGCCCTAAAATTGA+AGG	+	chr6.3:19214833-19214852	None:intergenic	40.0%
!	ATAAGGTTAGCCATGGCAAT+TGG	-	chr6.3:19215184-19215203	MS.gene49839:CDS	40.0%
!	GCTAAAAATGGTGGTGTTGA+TGG	-	chr6.3:19214902-19214921	MS.gene49839:CDS	40.0%
!!	ATTAAGAGCACCAATTGCCA+TGG	+	chr6.3:19215197-19215216	None:intergenic	40.0%
!!!	TTTTGATGGTAGGGGTACTT+TGG	-	chr6.3:19215074-19215093	MS.gene49839:CDS	40.0%
	CCTCTTGTTCTTTCAGCATG+GGG	-	chr6.3:19214992-19215011	MS.gene49839:CDS	45.0%
	CTCTTGTTCTTTCAGCATGG+GGG	-	chr6.3:19214993-19215012	MS.gene49839:CDS	45.0%
	TCTTGTTCTTTCAGCATGGG+GGG	-	chr6.3:19214994-19215013	MS.gene49839:CDS	45.0%
	TGTTTGTTTCCTTGGCTAGC+TGG	-	chr6.3:19214716-19214735	MS.gene49839:CDS	45.0%
!	GGTGCTATGGCTAAAAATGG+TGG	-	chr6.3:19214893-19214912	MS.gene49839:CDS	45.0%
!!	GCTGTTTCAGGTTGTTATGC+TGG	-	chr6.3:19214935-19214954	MS.gene49839:CDS	45.0%
!!	GGTGCACAAATTGCTGTTTC+AGG	-	chr6.3:19214923-19214942	MS.gene49839:CDS	45.0%
!!	TTGGCTTGGGGAAATTCTCT+AGG	-	chr6.3:19214854-19214873	MS.gene49839:CDS	45.0%
	CCCCATGCTGAAAGAACAAG+AGG	+	chr6.3:19214995-19215014	None:intergenic	50.0%
	GAAAATCCACCAGCTAGCCA+AGG	+	chr6.3:19214728-19214747	None:intergenic	50.0%
!	TTGTTTCCTTGGCTAGCTGG+TGG	-	chr6.3:19214719-19214738	MS.gene49839:CDS	50.0%
!!!	AGGGCTAACCGTTTTGGCTT+GGG	-	chr6.3:19214841-19214860	MS.gene49839:CDS	50.0%
!!!	TAGGGCTAACCGTTTTGGCT+TGG	-	chr6.3:19214840-19214859	MS.gene49839:CDS	50.0%
!!!	GGGCTAACCGTTTTGGCTTG+GGG	-	chr6.3:19214842-19214861	MS.gene49839:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	19214599	19215219	19214599	ID=MS.gene49839
chr6.3	mRNA	19214599	19215219	19214599	ID=MS.gene49839.t1;Parent=MS.gene49839
chr6.3	exon	19214599	19215219	19214599	ID=MS.gene49839.t1.exon1;Parent=MS.gene49839.t1
chr6.3	CDS	19214599	19215219	19214599	ID=cds.MS.gene49839.t1;Parent=MS.gene49839.t1

Gene Sequence

>MS.gene49839

ATGGAAAATGTGGATCCAAAGAGTAGCTTAGTTGCATATTTAACATCATCATTGATTGGAATTGTCTTTGGAAACATGGCATGTGTGACAACAAAGAGTACAAGACCACCAAGAAGATGTTTGTTTCCTTGGCTAGCTGGTGGATTTTCAATGAGTGTGACATGGACTTATATAATAGCTGAGGAACTTGTTTCACTTTTGATTTCAATTGGATATGTAATTGGTGTTAGTCCTTCAATTTTAGGGCTAACCGTTTTGGCTTGGGGAAATTCTCTAGGTGATTTGATAGCAAATGGTGCTATGGCTAAAAATGGTGGTGTTGATGGTGCACAAATTGCTGTTTCAGGTTGTTATGCTGGTCCTATGTTCAATATTTTGATGGGTTTAGGGTTACCTCTTGTTCTTTCAGCATGGGGGGAATACCCTAATTCTTACGTTGTTCCTAAGGATAGTTCACTTTTGGGGACAATATTGTTTTTGATGGTAGGGGTACTTTGGTCACTTGTTGTTTTAGTTAAGAAGAATATGAGGCTTGATAAGTTTTTAGGAGCTGGTCTCTTGACCATTTACCTTTGTTTCTTGTTTATAAGGTTAGCCATGGCAATTGGTGCTCTTAATTAA

Protein sequence

>MS.gene49839.t1

MENVDPKSSLVAYLTSSLIGIVFGNMACVTTKSTRPPRRCLFPWLAGGFSMSVTWTYIIAEELVSLLISIGYVIGVSPSILGLTVLAWGNSLGDLIANGAMAKNGGVDGAQIAVSGCYAGPMFNILMGLGLPLVLSAWGEYPNSYVVPKDSSLLGTILFLMVGVLWSLVVLVKKNMRLDKFLGAGLLTIYLCFLFIRLAMAIGALN