AlfalfaGEDB | MS.gene52153

M. sativa cultivar XinJiangDaYe / MS.gene52153

KEGG: K01358
GO: GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0016825 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00574.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52153.t1	XP_003607254.1	98.6	139	2	0	4	142	100	238	7.80E-72	279.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52153.t1	Q9FN42	83.5	139	23	0	4	142	103	241	5.7e-63	241.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52153.t1	B7FIP5	98.6	139	2	0	4	142	100	238	5.7e-72	279.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049341	MS.gene52153	0.808512	2.99E-50	-1.69E-46
MS.gene049382	MS.gene52153	0.822601	2.17E-53	-1.69E-46
MS.gene049703	MS.gene52153	-0.821419	4.08E-53	-1.69E-46
MS.gene049756	MS.gene52153	0.814783	1.29E-51	-1.69E-46
MS.gene04984	MS.gene52153	0.805638	1.21E-49	-1.69E-46
MS.gene050114	MS.gene52153	0.805071	1.59E-49	-1.69E-46
MS.gene050277	MS.gene52153	0.864364	1.28E-64	-1.69E-46
MS.gene050317	MS.gene52153	0.801842	7.44E-49	-1.69E-46
MS.gene050320	MS.gene52153	0.810928	9.03E-51	-1.69E-46
MS.gene050623	MS.gene52153	-0.815641	8.33E-52	-1.69E-46
MS.gene050838	MS.gene52153	0.82155	3.80E-53	-1.69E-46
MS.gene051063	MS.gene52153	0.807977	3.88E-50	-1.69E-46
MS.gene051243	MS.gene52153	0.807309	5.38E-50	-1.69E-46
MS.gene051245	MS.gene52153	0.867317	1.50E-65	-1.69E-46
MS.gene051328	MS.gene52153	0.804787	1.83E-49	-1.69E-46
MS.gene051746	MS.gene52153	0.803007	4.28E-49	-1.69E-46
MS.gene051748	MS.gene52153	0.819261	1.27E-52	-1.69E-46
MS.gene051757	MS.gene52153	0.812021	5.23E-51	-1.69E-46
MS.gene051903	MS.gene52153	0.807215	5.64E-50	-1.69E-46
MS.gene052286	MS.gene52153	0.822447	2.36E-53	-1.69E-46
MS.gene052434	MS.gene52153	0.800547	1.37E-48	-1.69E-46
MS.gene052856	MS.gene52153	0.818651	1.75E-52	-1.69E-46
MS.gene053058	MS.gene52153	0.824105	9.66E-54	-1.69E-46
MS.gene053109	MS.gene52153	0.84276	2.12E-58	-1.69E-46
MS.gene053216	MS.gene52153	0.804008	2.65E-49	-1.69E-46
MS.gene056069	MS.gene52153	-0.803563	3.28E-49	-1.69E-46
MS.gene056432	MS.gene52153	0.812381	4.36E-51	-1.69E-46
MS.gene056631	MS.gene52153	0.818128	2.30E-52	-1.69E-46
MS.gene056760	MS.gene52153	0.827259	1.73E-54	-1.69E-46
MS.gene057057	MS.gene52153	0.844243	8.53E-59	-1.69E-46
MS.gene057094	MS.gene52153	0.80278	4.77E-49	-1.69E-46
MS.gene057140	MS.gene52153	0.816962	4.22E-52	-1.69E-46
MS.gene057234	MS.gene52153	0.846216	2.50E-59	-1.69E-46
MS.gene057451	MS.gene52153	-0.824612	7.35E-54	-1.69E-46
MS.gene057452	MS.gene52153	-0.823315	1.48E-53	-1.69E-46
MS.gene05795	MS.gene52153	0.806328	8.68E-50	-1.69E-46
MS.gene058054	MS.gene52153	0.800602	1.33E-48	-1.69E-46
MS.gene058055	MS.gene52153	0.828468	8.84E-55	-1.69E-46
MS.gene058084	MS.gene52153	0.800724	1.26E-48	-1.69E-46
MS.gene058182	MS.gene52153	0.818796	1.63E-52	-1.69E-46
MS.gene058238	MS.gene52153	0.85479	9.73E-62	-1.69E-46
MS.gene058399	MS.gene52153	0.811197	7.90E-51	-1.69E-46
MS.gene058646	MS.gene52153	0.811569	6.56E-51	-1.69E-46
MS.gene058654	MS.gene52153	0.807672	4.51E-50	-1.69E-46
MS.gene058906	MS.gene52153	0.833731	4.51E-56	-1.69E-46
MS.gene059719	MS.gene52153	0.811024	8.61E-51	-1.69E-46
MS.gene05978	MS.gene52153	0.813558	2.41E-51	-1.69E-46
MS.gene06001	MS.gene52153	0.826885	2.12E-54	-1.69E-46
MS.gene060165	MS.gene52153	0.820191	7.81E-53	-1.69E-46
MS.gene060702	MS.gene52153	0.804511	2.09E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52153.t1	MTR_4g075100	98.561	139	2	0	4	142	100	238	5.89e-101	289
MS.gene52153.t1	MTR_1g086940	48.921	139	71	0	1	139	163	301	8.11e-41	138
MS.gene52153.t1	MTR_7g109610	46.809	141	75	0	1	141	160	300	5.65e-39	133
MS.gene52153.t1	MTR_7g082980	37.013	154	83	1	1	140	131	284	6.95e-33	117
MS.gene52153.t1	MTR_5g026840	40.876	137	78	1	1	134	140	276	1.86e-30	111
MS.gene52153.t1	MTR_4g069800	41.880	117	68	0	5	121	123	239	2.50e-29	107
MS.gene52153.t1	MTR_4g069800	41.880	117	68	0	5	121	155	271	5.68e-29	107
MS.gene52153.t1	MTR_0002s0730	40.833	120	70	1	4	122	77	196	1.27e-28	104
MS.gene52153.t1	MTR_4g034690	40.171	117	69	1	4	119	77	193	2.40e-28	103
MS.gene52153.t1	MTR_4g102000	39.416	137	78	2	5	141	186	317	4.93e-26	100
MS.gene52153.t1	MTR_7g112640	37.857	140	85	1	4	141	174	313	5.82e-26	100
MS.gene52153.t1	MTR_4g099360	45.299	117	64	0	4	120	167	283	7.61e-23	91.7
MS.gene52153.t1	MTR_4g069800	51.613	62	30	0	5	66	155	216	1.97e-16	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene52153.t1	AT5G23140	81.690	142	26	0	1	142	100	241	3.30e-86	252
MS.gene52153.t1	AT1G02560	50.833	120	59	0	1	120	166	285	7.25e-38	130
MS.gene52153.t1	ATCG00670	43.333	120	67	1	4	122	77	196	9.31e-33	114
MS.gene52153.t1	AT1G66670	42.149	121	70	0	1	121	137	257	1.44e-32	117
MS.gene52153.t1	AT4G17040	45.299	117	64	0	4	120	168	284	1.88e-30	111
MS.gene52153.t1	AT1G11750	41.880	117	68	0	5	121	152	268	2.81e-30	110
MS.gene52153.t1	AT5G45390	43.802	121	68	0	1	121	131	251	3.28e-30	110
MS.gene52153.t1	AT1G11750	41.880	117	68	0	5	121	170	286	5.10e-30	110
MS.gene52153.t1	AT1G09130	38.571	140	84	1	4	141	185	324	1.76e-28	107
MS.gene52153.t1	AT1G09130	38.571	140	84	1	4	141	185	324	1.76e-28	107
MS.gene52153.t1	AT1G09130	38.571	140	84	1	4	141	185	324	2.70e-28	107
MS.gene52153.t1	AT1G12410	39.695	131	77	1	1	131	141	269	3.43e-27	102
MS.gene52153.t1	AT1G49970	31.933	119	80	1	6	123	237	355	9.98e-12	61.6

Find 44 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCTTACCCTCCAATTCTAAT+TGG	0.195466	4.1:-29042973	None:intergenic
GCTCAAGAGGCAAAGGAGTT+TGG	0.292108	4.1:+29042870	MS.gene52153:CDS
CTCAAGAGGCAAAGGAGTTT+GGG	0.337555	4.1:+29042871	MS.gene52153:CDS
TGACGTAATTCAGAAGAATA+TGG	0.350320	4.1:+29042827	MS.gene52153:CDS
ATCATGATTCACCAGCCTTC+TGG	0.360193	4.1:+29042699	MS.gene52153:CDS
TCCGATCAACACAATCTGTT+TGG	0.364596	4.1:+29042599	MS.gene52153:CDS
TGATCAGAGACCTATGACTT+TGG	0.371036	4.1:+29042911	MS.gene52153:CDS
TTTGGGTCAGGCTGCGTCCA+TGG	0.373930	4.1:+29042617	MS.gene52153:CDS
GCTGAAGAAGGCAAAGATAA+AGG	0.396103	4.1:+29042948	MS.gene52153:CDS
CCGATCAACACAATCTGTTT+GGG	0.440075	4.1:+29042600	MS.gene52153:CDS
GGTTCTCTTCTGTTGTGTGC+AGG	0.449992	4.1:+29042639	MS.gene52153:CDS
TCTGTTGTGTGCAGGTGCCA+AGG	0.453852	4.1:+29042647	MS.gene52153:CDS
TCTGATGCTGTTGCTGAAGA+AGG	0.458298	4.1:+29042936	MS.gene52153:CDS
TTGGGTCAGGCTGCGTCCAT+GGG	0.459694	4.1:+29042618	MS.gene52153:CDS
TTCTTCTGAATTACGTCAAG+TGG	0.465824	4.1:-29042822	None:intergenic
GCATCAGAAACCAAAGTCAT+AGG	0.469025	4.1:-29042921	None:intergenic
CAACACAATCTGTTTGGGTC+AGG	0.479112	4.1:+29042605	MS.gene52153:CDS
CACTGTACCCACCAGAAGGC+TGG	0.496466	4.1:-29042710	None:intergenic
TTCTGGTGGGTACAGTGGCC+AGG	0.499106	4.1:+29042716	MS.gene52153:CDS
ATGATACTATGCAGTACATT+CGG	0.505359	4.1:+29042574	MS.gene52153:CDS
AAGAGCACGCCTCTGACCCT+TGG	0.513255	4.1:-29042664	None:intergenic
GAATTGCAATATCCTTTGCC+TGG	0.517374	4.1:-29042734	None:intergenic
CAGCCTTCTGGTGGGTACAG+TGG	0.521919	4.1:+29042711	MS.gene52153:CDS
TGAATCATGATTGTAGCATT+CGG	0.523340	4.1:-29042687	None:intergenic
CCAAGCAGATTGTTCGAATG+TGG	0.525727	4.1:+29042760	MS.gene52153:CDS
CTGTTGTGTGCAGGTGCCAA+GGG	0.539562	4.1:+29042648	MS.gene52153:CDS
GGAGTTTGGGATTATTGATG+AGG	0.549497	4.1:+29042884	MS.gene52153:CDS
TTACTTTATGACTGCTCAAG+AGG	0.554775	4.1:+29042857	MS.gene52153:CDS
TAATTCAGAAGAATATGGAT+AGG	0.558802	4.1:+29042832	MS.gene52153:CDS
GTGCAGGTGCCAAGGGTCAG+AGG	0.561637	4.1:+29042655	MS.gene52153:CDS
TGGGTACAGTGGCCAGGCAA+AGG	0.561919	4.1:+29042722	MS.gene52153:CDS
CCCAAACAGATTGTGTTGAT+CGG	0.562482	4.1:-29042600	None:intergenic
TCTGAATTACGTCAAGTGGT+TGG	0.569106	4.1:-29042818	None:intergenic
TGTCACTGGAAATGTGTGTA+GGG	0.571277	4.1:+29042541	None:intergenic
ATGATTCACCAGCCTTCTGG+TGG	0.571995	4.1:+29042702	MS.gene52153:CDS
AATTCAGAAGAATATGGATA+GGG	0.575900	4.1:+29042833	MS.gene52153:CDS
CCACATTCGAACAATCTGCT+TGG	0.578997	4.1:-29042760	None:intergenic
TATGACTGCTCAAGAGGCAA+AGG	0.586598	4.1:+29042863	MS.gene52153:CDS
GTGTCACTGGAAATGTGTGT+AGG	0.594431	4.1:+29042540	None:intergenic
CAAGCAGATTGTTCGAATGT+GGG	0.600409	4.1:+29042761	MS.gene52153:CDS
TGGCCACTGTACCCACCAGA+AGG	0.601246	4.1:-29042714	None:intergenic
GAGTTGTACGTCAAGCACAC+TGG	0.641757	4.1:+29042795	MS.gene52153:CDS
TGATTCACCAGCCTTCTGGT+GGG	0.661766	4.1:+29042703	MS.gene52153:CDS
ACACAACAGAAGAGAACCCA+TGG	0.683171	4.1:-29042634	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATTCAGAAGAATATGGATA+GGG	+	chr4.1:29042833-29042852	MS.gene52153:CDS	25.0%
!	TAATTCAGAAGAATATGGAT+AGG	+	chr4.1:29042832-29042851	MS.gene52153:CDS	25.0%
	ATGATACTATGCAGTACATT+CGG	+	chr4.1:29042574-29042593	MS.gene52153:CDS	30.0%
	TGAATCATGATTGTAGCATT+CGG	-	chr4.1:29042690-29042709	None:intergenic	30.0%
	TGACGTAATTCAGAAGAATA+TGG	+	chr4.1:29042827-29042846	MS.gene52153:CDS	30.0%
	TTACTTTATGACTGCTCAAG+AGG	+	chr4.1:29042857-29042876	MS.gene52153:CDS	35.0%
	TTCTTCTGAATTACGTCAAG+TGG	-	chr4.1:29042825-29042844	None:intergenic	35.0%
	CAAGCAGATTGTTCGAATGT+GGG	+	chr4.1:29042761-29042780	MS.gene52153:CDS	40.0%
	CCGATCAACACAATCTGTTT+GGG	+	chr4.1:29042600-29042619	MS.gene52153:CDS	40.0%
	GAATTGCAATATCCTTTGCC+TGG	-	chr4.1:29042737-29042756	None:intergenic	40.0%
	GCATCAGAAACCAAAGTCAT+AGG	-	chr4.1:29042924-29042943	None:intergenic	40.0%
	GCTGAAGAAGGCAAAGATAA+AGG	+	chr4.1:29042948-29042967	MS.gene52153:CDS	40.0%
	TCCGATCAACACAATCTGTT+TGG	+	chr4.1:29042599-29042618	MS.gene52153:CDS	40.0%
	TCTGAATTACGTCAAGTGGT+TGG	-	chr4.1:29042821-29042840	None:intergenic	40.0%
	TGATCAGAGACCTATGACTT+TGG	+	chr4.1:29042911-29042930	MS.gene52153:CDS	40.0%
!	CCCAAACAGATTGTGTTGAT+CGG	-	chr4.1:29042603-29042622	None:intergenic	40.0%
!	GGAGTTTGGGATTATTGATG+AGG	+	chr4.1:29042884-29042903	MS.gene52153:CDS	40.0%
	ACACAACAGAAGAGAACCCA+TGG	-	chr4.1:29042637-29042656	None:intergenic	45.0%
	ATCATGATTCACCAGCCTTC+TGG	+	chr4.1:29042699-29042718	MS.gene52153:CDS	45.0%
	CAACACAATCTGTTTGGGTC+AGG	+	chr4.1:29042605-29042624	MS.gene52153:CDS	45.0%
	CCAAGCAGATTGTTCGAATG+TGG	+	chr4.1:29042760-29042779	MS.gene52153:CDS	45.0%
	CCACATTCGAACAATCTGCT+TGG	-	chr4.1:29042763-29042782	None:intergenic	45.0%
	CTCAAGAGGCAAAGGAGTTT+GGG	+	chr4.1:29042871-29042890	MS.gene52153:CDS	45.0%
	TATGACTGCTCAAGAGGCAA+AGG	+	chr4.1:29042863-29042882	MS.gene52153:CDS	45.0%
	TCTGATGCTGTTGCTGAAGA+AGG	+	chr4.1:29042936-29042955	MS.gene52153:CDS	45.0%
	ATGATTCACCAGCCTTCTGG+TGG	+	chr4.1:29042702-29042721	MS.gene52153:CDS	50.0%
	GAGTTGTACGTCAAGCACAC+TGG	+	chr4.1:29042795-29042814	MS.gene52153:CDS	50.0%
	GCTCAAGAGGCAAAGGAGTT+TGG	+	chr4.1:29042870-29042889	MS.gene52153:CDS	50.0%
	GGTTCTCTTCTGTTGTGTGC+AGG	+	chr4.1:29042639-29042658	MS.gene52153:CDS	50.0%
	TGATTCACCAGCCTTCTGGT+GGG	+	chr4.1:29042703-29042722	MS.gene52153:CDS	50.0%
	CTGTTGTGTGCAGGTGCCAA+GGG	+	chr4.1:29042648-29042667	MS.gene52153:CDS	55.0%
	TCTGTTGTGTGCAGGTGCCA+AGG	+	chr4.1:29042647-29042666	MS.gene52153:CDS	55.0%
	CACTGTACCCACCAGAAGGC+TGG	-	chr4.1:29042713-29042732	None:intergenic	60.0%
	TGGCCACTGTACCCACCAGA+AGG	-	chr4.1:29042717-29042736	None:intergenic	60.0%
	TGGGTACAGTGGCCAGGCAA+AGG	+	chr4.1:29042722-29042741	MS.gene52153:CDS	60.0%
	TTGGGTCAGGCTGCGTCCAT+GGG	+	chr4.1:29042618-29042637	MS.gene52153:CDS	60.0%
	TTTGGGTCAGGCTGCGTCCA+TGG	+	chr4.1:29042617-29042636	MS.gene52153:CDS	60.0%
!	AAGAGCACGCCTCTGACCCT+TGG	-	chr4.1:29042667-29042686	None:intergenic	60.0%
!	CAGCCTTCTGGTGGGTACAG+TGG	+	chr4.1:29042711-29042730	MS.gene52153:CDS	60.0%
!	TTCTGGTGGGTACAGTGGCC+AGG	+	chr4.1:29042716-29042735	MS.gene52153:CDS	60.0%
	GTGCAGGTGCCAAGGGTCAG+AGG	+	chr4.1:29042655-29042674	MS.gene52153:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	29042552	29042980	29042552	ID=MS.gene52153
chr4.1	mRNA	29042552	29042980	29042552	ID=MS.gene52153.t1;Parent=MS.gene52153
chr4.1	exon	29042552	29042980	29042552	ID=MS.gene52153.t1.exon1;Parent=MS.gene52153.t1
chr4.1	CDS	29042552	29042980	29042552	ID=cds.MS.gene52153.t1;Parent=MS.gene52153.t1

Gene Sequence

>MS.gene52153

ATGTGTGTAGGGCTTGCAATATATGATACTATGCAGTACATTCGGTCTCCGATCAACACAATCTGTTTGGGTCAGGCTGCGTCCATGGGTTCTCTTCTGTTGTGTGCAGGTGCCAAGGGTCAGAGGCGTGCTCTTCCGAATGCTACAATCATGATTCACCAGCCTTCTGGTGGGTACAGTGGCCAGGCAAAGGATATTGCAATTCATACCAAGCAGATTGTTCGAATGTGGGATGCGCTGAATGAGTTGTACGTCAAGCACACTGGCCAACCACTTGACGTAATTCAGAAGAATATGGATAGGGATTACTTTATGACTGCTCAAGAGGCAAAGGAGTTTGGGATTATTGATGAGGTTATTGATCAGAGACCTATGACTTTGGTTTCTGATGCTGTTGCTGAAGAAGGCAAAGATAAAGGTTCCAATTAG

Protein sequence

>MS.gene52153.t1

MCVGLAIYDTMQYIRSPINTICLGQAASMGSLLLCAGAKGQRRALPNATIMIHQPSGGYSGQAKDIAIHTKQIVRMWDALNELYVKHTGQPLDVIQKNMDRDYFMTAQEAKEFGIIDEVIDQRPMTLVSDAVAEEGKDKGSN