AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52940.t1 MTR_2g038250 98.039 102 2 0 39 140 143 244 6.53e-71 213
MS.gene52940.t1 MTR_1g022385 98.039 102 2 0 39 140 174 275 1.90e-70 213
MS.gene52940.t1 MTR_4g008160 89.216 102 11 0 39 140 142 243 2.90e-65 199
MS.gene52940.t1 MTR_1g022375 76.829 82 19 0 49 130 75 156 2.96e-42 137
MS.gene52940.t1 MTR_7g059170 54.902 102 46 0 39 140 145 246 1.12e-40 136
MS.gene52940.t1 MTR_1g108785 75.676 37 8 1 97 132 23 59 1.58e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52940.t1 AT2G44120 85.294 102 15 0 39 140 141 242 2.91e-64 196
MS.gene52940.t1 AT2G44120 85.294 102 15 0 39 140 146 247 3.63e-64 196
MS.gene52940.t1 AT2G01250 86.275 102 14 0 39 140 141 242 5.43e-64 195
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT3G13580 84.314 102 16 0 39 140 143 244 5.23e-63 193
MS.gene52940.t1 AT1G80750 52.941 102 47 1 40 140 146 247 1.05e-30 110
MS.gene52940.t1 AT2G01250 72.973 37 10 0 39 75 141 177 5.02e-11 58.2

Find 37 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCATTAGAAGGATGAATT+AGG 0.270598 1.1:-57004894 None:intergenic
TTTCAAGCTCAAAGCTCCTT+TGG 0.284641 1.1:-57004990 MS.gene52940:CDS
AGTGTTCAATTGCAGGTTCT+TGG 0.317764 1.1:-57005103 MS.gene52940:intron
TTCAAGCTCAAAGCTCCTTT+GGG 0.329811 1.1:-57004989 MS.gene52940:CDS
TTGATCTACAAGCGCGGTTA+TGG 0.337108 1.1:-57005452 MS.gene52940:CDS
TCAATTCTCTAACACTCTTC+AGG 0.373375 1.1:+57005471 None:intergenic
AAGGTGGTGATGCTGGTAAC+AGG 0.401808 1.1:-57004934 MS.gene52940:CDS
GTGTTCAATTGCAGGTTCTT+GGG 0.403327 1.1:-57005102 MS.gene52940:intron
ACATCAATGAACTCATTAGA+AGG 0.462677 1.1:-57004904 MS.gene52940:CDS
GAAGCGTGAAGCTAAGCTTA+AGG 0.464202 1.1:-57005511 MS.gene52940:intron
AAGCGTGAAGCTAAGCTTAA+GGG 0.467088 1.1:-57005510 MS.gene52940:CDS
AGATAACTCTATCATTGAAC+AGG 0.482014 1.1:-57005399 MS.gene52940:intron
TTTATGCATCTACGGAGGTC+AGG 0.484893 1.1:-57005892 MS.gene52940:CDS
AAGAGAAATCACTATGTTGA+AGG 0.489111 1.1:-57004953 MS.gene52940:CDS
AAAGGAGCTTTGAGCTTGAA+AGG 0.504831 1.1:+57004992 None:intergenic
GAGAGGCAAACAACTTCCTA+TGG 0.504947 1.1:-57005015 MS.gene52940:CDS
TATGTTGAAGGTGGTGATGC+TGG 0.533299 1.1:-57004941 MS.gene52940:CDS
AAGCTGAGAAAGAACATGGT+TGG 0.534698 1.1:-57005837 MS.gene52940:CDS
TTGCAGGTTCTTGGGAAACA+TGG 0.547799 1.1:-57005094 MS.gene52940:intron
ATCCACGAGATCATGACTGT+TGG 0.549865 1.1:-57005049 MS.gene52940:CDS
GCGGTTATGGAAAGGTTGAT+AGG 0.549876 1.1:-57005439 MS.gene52940:CDS
TTCTTCTTCATGCCACCCAA+AGG 0.554983 1.1:+57004974 None:intergenic
TGACAGGGTTTATGCATCTA+CGG 0.562090 1.1:-57005900 None:intergenic
CTTTGAGCTTGAAAGGCCAT+AGG 0.568033 1.1:+57004999 None:intergenic
AAGCTCAAAGCTCCTTTGGG+TGG 0.570830 1.1:-57004986 MS.gene52940:CDS
ATCCAACAGTCATGATCTCG+TGG 0.585385 1.1:+57005047 None:intergenic
CTACAAGCGCGGTTATGGAA+AGG 0.599869 1.1:-57005447 MS.gene52940:CDS
CGTGAAGCTAAGCTTAAGGG+TGG 0.634387 1.1:-57005507 MS.gene52940:CDS
GAGAAAGCTGAGAAAGAACA+TGG 0.636998 1.1:-57005841 MS.gene52940:CDS
AGAAATCACTATGTTGAAGG+TGG 0.637998 1.1:-57004950 MS.gene52940:CDS
GAGGTCAGGAGAGGCTACAG+AGG 0.640976 1.1:-57005878 MS.gene52940:CDS
AGGTGGTGATGCTGGTAACA+GGG 0.651466 1.1:-57004933 MS.gene52940:CDS
GCATCTACGGAGGTCAGGAG+AGG 0.664972 1.1:-57005887 MS.gene52940:CDS
AGAGAATTGATCTACAAGCG+CGG 0.721136 1.1:-57005458 MS.gene52940:CDS
TGTTGGATCTCACTTCAGAG+AGG 0.724978 1.1:-57005032 MS.gene52940:CDS
TGGTGATGCTGGTAACAGGG+AGG 0.745630 1.1:-57004930 MS.gene52940:CDS
CAGGGTTTATGCATCTACGG+AGG 0.767701 1.1:-57005897 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAATAGAAAAATTTAAC+CGG + chr1.1:57005652-57005671 None:intergenic 15.0%
!!! AATGTTTTAGAATTTTAGAA+TGG - chr1.1:57005211-57005230 MS.gene52940:intron 15.0%
!!! AATTTAAAGTACGTATTTGT+GGG - chr1.1:57005128-57005147 MS.gene52940:intron 20.0%
!!! TAATTTAAAGTACGTATTTG+TGG - chr1.1:57005127-57005146 MS.gene52940:intron 20.0%
!!! TTCTTTCTTTGACTTATATA+TGG - chr1.1:57005061-57005080 MS.gene52940:CDS 20.0%
!!! TTTAGAATTTTAGAATGGTA+AGG - chr1.1:57005216-57005235 MS.gene52940:intron 20.0%
! CTTTCATATTTGTTCTTGTA+GGG - chr1.1:57005096-57005115 MS.gene52940:intron 25.0%
! GTTTATCCAATTAATAGTGA+GGG - chr1.1:57005168-57005187 MS.gene52940:intron 25.0%
! TGTTTATCCAATTAATAGTG+AGG - chr1.1:57005167-57005186 MS.gene52940:intron 25.0%
AAGAGAAATCACTATGTTGA+AGG - chr1.1:57005831-57005850 MS.gene52940:intron 30.0%
ACATCAATGAACTCATTAGA+AGG - chr1.1:57005880-57005899 MS.gene52940:CDS 30.0%
AGATAACTCTATCATTGAAC+AGG - chr1.1:57005385-57005404 MS.gene52940:intron 30.0%
AGTCAAAGAAAGAATCTCAT+TGG + chr1.1:57005055-57005074 None:intergenic 30.0%
GCTTTCATATTTGTTCTTGT+AGG - chr1.1:57005095-57005114 MS.gene52940:intron 30.0%
!! GGTGCTATTATTATTGTTGA+AGG - chr1.1:57005618-57005637 MS.gene52940:intron 30.0%
!!! CTTTGAAACATCCTTTTCTA+GGG - chr1.1:57004999-57005018 MS.gene52940:CDS 30.0%
!!! TCTTTGAAACATCCTTTTCT+AGG - chr1.1:57004998-57005017 MS.gene52940:CDS 30.0%
AACAACTCACATTCTGACTT+TGG + chr1.1:57005523-57005542 None:intergenic 35.0%
AATAATAGCACCACCATACA+GGG + chr1.1:57005610-57005629 None:intergenic 35.0%
ACATTCTGACTTTGGCAAAT+TGG + chr1.1:57005515-57005534 None:intergenic 35.0%
AGAAATCACTATGTTGAAGG+TGG - chr1.1:57005834-57005853 MS.gene52940:intron 35.0%
ATCCAATTAATAGTGAGGGA+TGG - chr1.1:57005172-57005191 MS.gene52940:intron 35.0%
ATTGTTGAAGGTTATGTTGC+CGG - chr1.1:57005630-57005649 MS.gene52940:intron 35.0%
CATTCTGACTTTGGCAAATT+GGG + chr1.1:57005514-57005533 None:intergenic 35.0%
CTGAACTGTATGACATAGAT+GGG - chr1.1:57005459-57005478 MS.gene52940:CDS 35.0%
GTGATAACTACATCCAATGA+GGG + chr1.1:57005028-57005047 None:intergenic 35.0%
TAATAATAGCACCACCATAC+AGG + chr1.1:57005611-57005630 None:intergenic 35.0%
TACCATCCCTCACTATTAAT+TGG + chr1.1:57005177-57005196 None:intergenic 35.0%
TCAATTCTCTAACACTCTTC+AGG + chr1.1:57005316-57005335 None:intergenic 35.0%
TCTGAACTGTATGACATAGA+TGG - chr1.1:57005458-57005477 MS.gene52940:CDS 35.0%
TGTGATAACTACATCCAATG+AGG + chr1.1:57005029-57005048 None:intergenic 35.0%
AAGCGTGAAGCTAAGCTTAA+GGG - chr1.1:57005274-57005293 MS.gene52940:intron 40.0%
AAGCTGAGAAAGAACATGGT+TGG - chr1.1:57004947-57004966 MS.gene52940:CDS 40.0%
AATGAGGGATTCCCTAGAAA+AGG + chr1.1:57005013-57005032 None:intergenic 40.0%
AGAGAATTGATCTACAAGCG+CGG - chr1.1:57005326-57005345 MS.gene52940:intron 40.0%
AGTGTTCAATTGCAGGTTCT+TGG - chr1.1:57005681-57005700 MS.gene52940:intron 40.0%
ATAATAGCACCACCATACAG+GGG + chr1.1:57005609-57005628 None:intergenic 40.0%
CTGACATAGTGTTCAATTGC+AGG - chr1.1:57005674-57005693 MS.gene52940:intron 40.0%
GAGAAAGCTGAGAAAGAACA+TGG - chr1.1:57004943-57004962 MS.gene52940:CDS 40.0%
GGAATACGATGATTCGCAAA+AGG - chr1.1:57005237-57005256 MS.gene52940:intron 40.0%
GTGTTCAATTGCAGGTTCTT+GGG - chr1.1:57005682-57005701 MS.gene52940:intron 40.0%
TGTGTGATCGAATGAAAGCA+GGG + chr1.1:57005430-57005449 None:intergenic 40.0%
TTCAAGCTCAAAGCTCCTTT+GGG - chr1.1:57005795-57005814 MS.gene52940:intron 40.0%
TTTCAAGCTCAAAGCTCCTT+TGG - chr1.1:57005794-57005813 MS.gene52940:intron 40.0%
!! AAAGGAGCTTTGAGCTTGAA+AGG + chr1.1:57005795-57005814 None:intergenic 40.0%
!! TTTTCTAGGGAATCCCTCAT+TGG - chr1.1:57005012-57005031 MS.gene52940:CDS 40.0%
ATCCAACAGTCATGATCTCG+TGG + chr1.1:57005740-57005759 None:intergenic 45.0%
ATCCACGAGATCATGACTGT+TGG - chr1.1:57005735-57005754 MS.gene52940:intron 45.0%
CTGTGTGATCGAATGAAAGC+AGG + chr1.1:57005431-57005450 None:intergenic 45.0%
CTTTGAGCTTGAAAGGCCAT+AGG + chr1.1:57005788-57005807 None:intergenic 45.0%
GAAGCGTGAAGCTAAGCTTA+AGG - chr1.1:57005273-57005292 MS.gene52940:intron 45.0%
GAGAGGCAAACAACTTCCTA+TGG - chr1.1:57005769-57005788 MS.gene52940:intron 45.0%
TGTTGGATCTCACTTCAGAG+AGG - chr1.1:57005752-57005771 MS.gene52940:intron 45.0%
TTCTTCTTCATGCCACCCAA+AGG + chr1.1:57005813-57005832 None:intergenic 45.0%
TTGATCTACAAGCGCGGTTA+TGG - chr1.1:57005332-57005351 MS.gene52940:intron 45.0%
TTGCAGGTTCTTGGGAAACA+TGG - chr1.1:57005690-57005709 MS.gene52940:intron 45.0%
! GCGGTTATGGAAAGGTTGAT+AGG - chr1.1:57005345-57005364 MS.gene52940:intron 45.0%
! TATGTTGAAGGTGGTGATGC+TGG - chr1.1:57005843-57005862 MS.gene52940:CDS 45.0%
AAGCTCAAAGCTCCTTTGGG+TGG - chr1.1:57005798-57005817 MS.gene52940:intron 50.0%
CGTGAAGCTAAGCTTAAGGG+TGG - chr1.1:57005277-57005296 MS.gene52940:intron 50.0%
CTACAAGCGCGGTTATGGAA+AGG - chr1.1:57005337-57005356 MS.gene52940:intron 50.0%
CTTGACCAATGTCCCCTGTA+TGG - chr1.1:57005594-57005613 MS.gene52940:intron 50.0%
! AAGGTGGTGATGCTGGTAAC+AGG - chr1.1:57005850-57005869 MS.gene52940:CDS 50.0%
! AGGTGGTGATGCTGGTAACA+GGG - chr1.1:57005851-57005870 MS.gene52940:CDS 50.0%
CACCACCATACAGGGGACAT+TGG + chr1.1:57005602-57005621 None:intergenic 55.0%
GACCAATGTCCCCTGTATGG+TGG - chr1.1:57005597-57005616 MS.gene52940:intron 55.0%
! TGGTGATGCTGGTAACAGGG+AGG - chr1.1:57005854-57005873 MS.gene52940:CDS 55.0%
GAGGTCAGGAGAGGCTACAG+AGG - chr1.1:57004906-57004925 MS.gene52940:CDS 60.0%
GCATCTACGGAGGTCAGGAG+AGG - chr1.1:57004897-57004916 MS.gene52940:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 57004895 57005911 57004895 ID=MS.gene52940
chr1.1 mRNA 57004895 57005911 57004895 ID=MS.gene52940.t1;Parent=MS.gene52940
chr1.1 exon 57005835 57005911 57005835 ID=MS.gene52940.t1.exon1;Parent=MS.gene52940.t1
chr1.1 CDS 57005835 57005911 57005835 ID=cds.MS.gene52940.t1;Parent=MS.gene52940.t1
chr1.1 exon 57005400 57005529 57005400 ID=MS.gene52940.t1.exon2;Parent=MS.gene52940.t1
chr1.1 CDS 57005400 57005529 57005400 ID=cds.MS.gene52940.t1;Parent=MS.gene52940.t1
chr1.1 exon 57004895 57005110 57004895 ID=MS.gene52940.t1.exon3;Parent=MS.gene52940.t1
chr1.1 CDS 57004895 57005110 57004895 ID=cds.MS.gene52940.t1;Parent=MS.gene52940.t1
Gene Sequence

>MS.gene52940

ATGCATCTACGGAGGTCAGGAGAGGCTACAGAGGTTTTTGCATCTACAGAGAAAGCTGAGAAAGAACATGGTTGGAAGTAAGTACTTGTGTCTCGAATATTTTTCTTTGAAACATCCTTTTCTAGGGAATCCCTCATTGGATGTAGTTATCACAAGACCAATGAGATTCTTTCTTTGACTTATATATGGTAATCAACTCTGCTTTCATATTTGTTCTTGTAGGGAAGATTTATAATTTAAAGTACGTATTTGTGGGTGTCTTTTACTCTTTATGTTTATCCAATTAATAGTGAGGGATGGTACTTTAATGTTTTAGAATGTTTTAGAATTTTAGAATGGTAAGGAATACGATGATTCGCAAAAGGAGTTGATTGCGTTGAAGCGTGAAGCTAAGCTTAAGGGTGGATTTTATGTCGATCCTGAAGAGTGTTAGAGAATTGATCTACAAGCGCGGTTATGGAAAGGTTGATAGGCAAAGAATTGCTTTGACAGATAACTCTATCATTGAACAGGTATGTCTTTGATTAGTATTCCCTGCTTTCATTCGATCACACAGTTGTCAATCTGAACTGTATGACATAGATGGGATCTCGCTTCATTCTGACTCTGTACTTTACCCAATTTGCCAAAGTCAGAATGTGAGTTGTTTAAACTTGACTAGACAGTCTAGATTACGTGACTTTGTCTAATGTGTAATTTCTTGACCAATGTCCCCTGTATGGTGGTGCTATTATTATTGTTGAAGGTTATGTTGCCGGTTAAATTTTTCTATTTTTCATCTGACATAGTGTTCAATTGCAGGTTCTTGGGAAACATGGTATCATTTGCATTGAAGATCTCATCCACGAGATCATGACTGTTGGATCTCACTTCAGAGAGGCAAACAACTTCCTATGGCCTTTCAAGCTCAAAGCTCCTTTGGGTGGCATGAAGAAGAAGAGAAATCACTATGTTGAAGGTGGTGATGCTGGTAACAGGGAGGATTACATCAATGAACTCATTAGAAGGATGAATTAG

Protein sequence

>MS.gene52940.t1

MHLRRSGEATEVFASTEKAEKEHGWNVKLSLRVDFMSILKSVRELIYKRGYGKVDRQRIALTDNSIIEQVLGKHGIICIEDLIHEIMTVGSHFREANNFLWPFKLKAPLGGMKKKRNHYVEGGDAGNREDYINELIRRMN