AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53494


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53494.t1 MTR_8g089300 95.876 194 8 0 1 194 1 194 5.61e-127 355
MS.gene53494.t1 MTR_0097s0070 75.773 194 46 1 1 193 1 194 1.98e-97 281
MS.gene53494.t1 MTR_4g118810 36.111 144 90 2 28 170 35 177 3.66e-25 97.1
MS.gene53494.t1 MTR_0004s0210 35.185 162 89 2 28 189 10 155 1.11e-23 92.4
MS.gene53494.t1 MTR_2g020060 35.260 173 92 4 30 194 27 187 2.13e-23 92.4
MS.gene53494.t1 MTR_7g108130 32.778 180 114 3 16 194 18 191 2.24e-23 92.4
MS.gene53494.t1 MTR_4g081880 29.630 189 106 6 9 184 6 180 2.72e-16 73.6
MS.gene53494.t1 MTR_4g118800 30.159 126 87 1 64 188 56 181 2.81e-16 73.6
MS.gene53494.t1 MTR_7g011090 28.387 155 104 1 30 184 32 179 8.16e-16 72.4
MS.gene53494.t1 MTR_5g041900 28.387 155 104 1 30 184 32 179 5.18e-13 64.7
MS.gene53494.t1 MTR_1g058540 29.861 144 92 3 30 172 42 177 5.30e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53494.t1 AT5G44550 53.714 175 81 0 19 193 11 185 5.24e-61 189
MS.gene53494.t1 AT4G20390 55.491 173 76 1 23 194 14 186 2.38e-55 174
MS.gene53494.t1 AT4G15610 33.803 142 89 3 30 170 33 170 1.48e-20 85.1
MS.gene53494.t1 AT1G03700 30.189 159 95 2 28 186 11 153 1.09e-19 82.0
MS.gene53494.t1 AT4G15630 31.481 162 109 2 28 188 29 189 1.23e-19 82.4
MS.gene53494.t1 AT1G14160 32.121 165 102 2 30 194 55 209 3.87e-19 81.6
MS.gene53494.t1 AT4G15620 29.341 167 116 2 23 188 24 189 1.12e-18 80.1
MS.gene53494.t1 AT5G15290 30.114 176 108 4 1 171 1 166 1.86e-17 76.6
MS.gene53494.t1 AT3G06390 31.061 132 87 2 31 161 42 170 3.20e-14 68.6
MS.gene53494.t1 AT4G03540 28.244 131 78 2 43 173 26 140 1.53e-13 65.9

Find 45 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTCAGTCTTCATAATT+TGG 0.186212 4.2:+12263046 MS.gene53494:CDS
GCCTTAATAGCTTCTTTCAT+TGG 0.241559 4.2:+12263383 MS.gene53494:CDS
GCAGTAAGAGGAACTGTAAT+TGG 0.269342 4.2:-12262830 None:intergenic
AAAAGGATCCAATCCTTCTT+TGG 0.280238 4.2:-12262704 None:intergenic
CCCAATGAAAGAAGCTATTA+AGG 0.288413 4.2:-12263384 None:intergenic
TCCTTCTTTGGTTTGGTCTC+AGG 0.290070 4.2:-12262692 None:intergenic
AGCAACATTTATGTCAGAAT+TGG 0.314088 4.2:+12263283 MS.gene53494:CDS
GGTAGCAACTACAAAGCTTT+TGG 0.318946 4.2:-12262795 None:intergenic
ATGATAGCAATGGATATATT+AGG 0.342029 4.2:+12263071 MS.gene53494:CDS
CCTTAATAGCTTCTTTCATT+GGG 0.354084 4.2:+12263384 MS.gene53494:CDS
ATGGTAATTCACATGCAAAA+TGG 0.384012 4.2:+12263312 MS.gene53494:CDS
TTGGGTGAATTAGATGGCTT+TGG 0.384488 4.2:+12263235 MS.gene53494:intron
GTTAATTGCGATTTAGTTTG+AGG 0.384618 4.2:-12263451 None:intergenic
GATCCAATCCTTCTTTGGTT+TGG 0.385867 4.2:-12262699 None:intergenic
CAGTAAGAGGAACTGTAATT+GGG 0.394934 4.2:-12262829 None:intergenic
TAGTTTGAGGAGTTTGTTGA+TGG 0.399777 4.2:-12263438 None:intergenic
GCAACATTTATGTCAGAATT+GGG 0.419599 4.2:+12263284 MS.gene53494:CDS
GACTCAAATTTCATAACAAA+GGG 0.455425 4.2:+12263093 MS.gene53494:CDS
TCCTGAGACCAAACCAAAGA+AGG 0.459469 4.2:+12262691 MS.gene53494:CDS
TGCTGAAACTTGGCAGTAAG+AGG 0.478796 4.2:-12262842 None:intergenic
ACCACCACCTCTGTTGCAAT+AGG 0.484004 4.2:-12263360 None:intergenic
CAACCACCCTAAGGGACAAA+AGG 0.486521 4.2:-12262721 None:intergenic
TTATGGACTAACACAAAAGC+TGG 0.487148 4.2:-12262869 None:intergenic
AGCTGGAGTATGCTGAAACT+TGG 0.488766 4.2:-12262852 None:intergenic
AGACCAAACCAAAGAAGGAT+TGG 0.490966 4.2:+12262696 MS.gene53494:CDS
AGCTTTGTAGTTGCTACCAT+TGG 0.493742 4.2:+12262800 MS.gene53494:CDS
GGACTCAAATTTCATAACAA+AGG 0.497208 4.2:+12263092 MS.gene53494:CDS
ATGTCAGAATTGGGAAGGAA+TGG 0.507856 4.2:+12263293 MS.gene53494:CDS
TACACTATCACAATGGCCTC+AGG 0.512074 4.2:+12262632 None:intergenic
TAATTTGGTTATGATAGCAA+TGG 0.531106 4.2:+12263061 MS.gene53494:CDS
ATGAAGACTGACAATTCCAT+TGG 0.535909 4.2:-12263039 None:intergenic
TCCTATTGCAACAGAGGTGG+TGG 0.560694 4.2:+12263359 MS.gene53494:CDS
AGGTACTTTGTGGTAGCCAA+TGG 0.565693 4.2:+12263023 MS.gene53494:intron
GCTCTAGCATCAAGTGGAGA+TGG 0.573326 4.2:+12263257 MS.gene53494:CDS
TGTAATTGGGGTGCTACCAA+TGG 0.595649 4.2:-12262816 None:intergenic
TTTGAGTCCTATTGCAACAG+AGG 0.599496 4.2:+12263353 MS.gene53494:CDS
CATTTATGTCAGAATTGGGA+AGG 0.599985 4.2:+12263288 MS.gene53494:CDS
ATACTGCAATCAGTGCAAGT+TGG 0.603365 4.2:-12263118 None:intergenic
GAGTCCTATTGCAACAGAGG+TGG 0.611119 4.2:+12263356 MS.gene53494:CDS
AACAAGTGTAGCTGATACAG+TGG 0.613356 4.2:-12262753 None:intergenic
ACATCACTGTAATAATGAGT+AGG 0.627687 4.2:-12263415 None:intergenic
GTGAGAATACAGGTACTTTG+TGG 0.628246 4.2:+12263013 MS.gene53494:intron
GCTTTGGCTCTAGCATCAAG+TGG 0.633064 4.2:+12263251 MS.gene53494:CDS
AGTAAGAGGAACTGTAATTG+GGG 0.643245 4.2:-12262828 None:intergenic
ACTGATTGCAGTATTTGACA+CGG 0.674366 4.2:+12263127 MS.gene53494:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAATTAGAGAGATTTTA+TGG - chr4.2:12262889-12262908 None:intergenic 15.0%
!! TAACTTAAATGATCAATTAG+AGG - chr4.2:12263169-12263188 None:intergenic 20.0%
! AATGAAGAAAAAATAGAGTC+TGG + chr4.2:12262656-12262675 MS.gene53494:CDS 25.0%
! AATTGTCAGTCTTCATAATT+TGG + chr4.2:12263046-12263065 MS.gene53494:CDS 25.0%
! ATGATAGCAATGGATATATT+AGG + chr4.2:12263071-12263090 MS.gene53494:CDS 25.0%
! GACTCAAATTTCATAACAAA+GGG + chr4.2:12263093-12263112 MS.gene53494:CDS 25.0%
! TAATTTGGTTATGATAGCAA+TGG + chr4.2:12263061-12263080 MS.gene53494:CDS 25.0%
! TCACCACAAATTAAATCATA+AGG - chr4.2:12262997-12263016 None:intergenic 25.0%
!!! TATTTTTTCTTCATTTCCTG+AGG - chr4.2:12262651-12262670 None:intergenic 25.0%
!!! TCACCTTATGATTTAATTTG+TGG + chr4.2:12262991-12263010 MS.gene53494:intron 25.0%
ACATCACTGTAATAATGAGT+AGG - chr4.2:12263418-12263437 None:intergenic 30.0%
AGCAACATTTATGTCAGAAT+TGG + chr4.2:12263283-12263302 MS.gene53494:CDS 30.0%
ATGGTAATTCACATGCAAAA+TGG + chr4.2:12263312-12263331 MS.gene53494:CDS 30.0%
CCTTAATAGCTTCTTTCATT+GGG + chr4.2:12263384-12263403 MS.gene53494:CDS 30.0%
GATTAATTGGGTGAATTAGA+TGG + chr4.2:12263229-12263248 MS.gene53494:intron 30.0%
GCAACATTTATGTCAGAATT+GGG + chr4.2:12263284-12263303 MS.gene53494:CDS 30.0%
GGACTCAAATTTCATAACAA+AGG + chr4.2:12263092-12263111 MS.gene53494:CDS 30.0%
!! TAGATACAGAAGTGTTAGAA+AGG - chr4.2:12262912-12262931 None:intergenic 30.0%
!! TTAATTTGTGGTGAGAATAC+AGG + chr4.2:12263003-12263022 MS.gene53494:intron 30.0%
AAAAGGATCCAATCCTTCTT+TGG - chr4.2:12262707-12262726 None:intergenic 35.0%
ACTGATTGCAGTATTTGACA+CGG + chr4.2:12263127-12263146 MS.gene53494:CDS 35.0%
AGTAAGAGGAACTGTAATTG+GGG - chr4.2:12262831-12262850 None:intergenic 35.0%
ATGAAGACTGACAATTCCAT+TGG - chr4.2:12263042-12263061 None:intergenic 35.0%
CAGTAAGAGGAACTGTAATT+GGG - chr4.2:12262832-12262851 None:intergenic 35.0%
CATTTATGTCAGAATTGGGA+AGG + chr4.2:12263288-12263307 MS.gene53494:CDS 35.0%
CCCAATGAAAGAAGCTATTA+AGG - chr4.2:12263387-12263406 None:intergenic 35.0%
GCCTTAATAGCTTCTTTCAT+TGG + chr4.2:12263383-12263402 MS.gene53494:CDS 35.0%
! TTATGGACTAACACAAAAGC+TGG - chr4.2:12262872-12262891 None:intergenic 35.0%
!! TAGTTTGAGGAGTTTGTTGA+TGG - chr4.2:12263441-12263460 None:intergenic 35.0%
AACAAGTGTAGCTGATACAG+TGG - chr4.2:12262756-12262775 None:intergenic 40.0%
AGACCAAACCAAAGAAGGAT+TGG + chr4.2:12262696-12262715 MS.gene53494:CDS 40.0%
ATACTGCAATCAGTGCAAGT+TGG - chr4.2:12263121-12263140 None:intergenic 40.0%
ATGTCAGAATTGGGAAGGAA+TGG + chr4.2:12263293-12263312 MS.gene53494:CDS 40.0%
CAAAAATGCAACCACCCTAA+GGG - chr4.2:12262732-12262751 None:intergenic 40.0%
GATCCAATCCTTCTTTGGTT+TGG - chr4.2:12262702-12262721 None:intergenic 40.0%
GCAGTAAGAGGAACTGTAAT+TGG - chr4.2:12262833-12262852 None:intergenic 40.0%
GTGAGAATACAGGTACTTTG+TGG + chr4.2:12263013-12263032 MS.gene53494:intron 40.0%
TTGGGTGAATTAGATGGCTT+TGG + chr4.2:12263235-12263254 MS.gene53494:intron 40.0%
TTTGAGTCCTATTGCAACAG+AGG + chr4.2:12263353-12263372 MS.gene53494:CDS 40.0%
! AGCTTTGTAGTTGCTACCAT+TGG + chr4.2:12262800-12262819 MS.gene53494:CDS 40.0%
! ATTGGATCCTTTTGTCCCTT+AGG + chr4.2:12262714-12262733 MS.gene53494:CDS 40.0%
! GGTAGCAACTACAAAGCTTT+TGG - chr4.2:12262798-12262817 None:intergenic 40.0%
! TTGGATCCTTTTGTCCCTTA+GGG + chr4.2:12262715-12262734 MS.gene53494:CDS 40.0%
AGCTGGAGTATGCTGAAACT+TGG - chr4.2:12262855-12262874 None:intergenic 45.0%
AGGTACTTTGTGGTAGCCAA+TGG + chr4.2:12263023-12263042 MS.gene53494:intron 45.0%
CCAAAAATGCAACCACCCTA+AGG - chr4.2:12262733-12262752 None:intergenic 45.0%
TCCTGAGACCAAACCAAAGA+AGG + chr4.2:12262691-12262710 MS.gene53494:CDS 45.0%
TGCTGAAACTTGGCAGTAAG+AGG - chr4.2:12262845-12262864 None:intergenic 45.0%
! CCTTAGGGTGGTTGCATTTT+TGG + chr4.2:12262730-12262749 MS.gene53494:CDS 45.0%
! TGTAATTGGGGTGCTACCAA+TGG - chr4.2:12262819-12262838 None:intergenic 45.0%
!! TCCTTCTTTGGTTTGGTCTC+AGG - chr4.2:12262695-12262714 None:intergenic 45.0%
!!! ATTATATTTTTTTGATTAAT+TGG + chr4.2:12263216-12263235 MS.gene53494:intron 5.0%
!!! TTATATTTTTTTGATTAATT+GGG + chr4.2:12263217-12263236 MS.gene53494:intron 5.0%
ACCACCACCTCTGTTGCAAT+AGG - chr4.2:12263363-12263382 None:intergenic 50.0%
CAACCACCCTAAGGGACAAA+AGG - chr4.2:12262724-12262743 None:intergenic 50.0%
GAGTCCTATTGCAACAGAGG+TGG + chr4.2:12263356-12263375 MS.gene53494:CDS 50.0%
GCTCTAGCATCAAGTGGAGA+TGG + chr4.2:12263257-12263276 MS.gene53494:CDS 50.0%
TCCTATTGCAACAGAGGTGG+TGG + chr4.2:12263359-12263378 MS.gene53494:CDS 50.0%
! GATCCTTTTGTCCCTTAGGG+TGG + chr4.2:12262718-12262737 MS.gene53494:CDS 50.0%
!! GCTTTGGCTCTAGCATCAAG+TGG + chr4.2:12263251-12263270 MS.gene53494:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 12262644 12263472 12262644 ID=MS.gene53494
chr4.2 mRNA 12262644 12263472 12262644 ID=MS.gene53494.t1;Parent=MS.gene53494
chr4.2 exon 12262644 12262879 12262644 ID=MS.gene53494.t1.exon1;Parent=MS.gene53494.t1
chr4.2 CDS 12262644 12262879 12262644 ID=cds.MS.gene53494.t1;Parent=MS.gene53494.t1
chr4.2 exon 12263025 12263148 12263025 ID=MS.gene53494.t1.exon2;Parent=MS.gene53494.t1
chr4.2 CDS 12263025 12263148 12263025 ID=cds.MS.gene53494.t1;Parent=MS.gene53494.t1
chr4.2 exon 12263248 12263472 12263248 ID=MS.gene53494.t1.exon3;Parent=MS.gene53494.t1
chr4.2 CDS 12263248 12263472 12263248 ID=cds.MS.gene53494.t1;Parent=MS.gene53494.t1
Gene Sequence

>MS.gene53494

ATGGCCTCAGGAAATGAAGAAAAAATAGAGTCTGGTTTCAATGTTGTTCCTGAGACCAAACCAAAGAAGGATTGGATCCTTTTGTCCCTTAGGGTGGTTGCATTTTTGGCCACTGTATCAGCTACACTTGTTATGTCACTCAACAAACAAACCAAAAGCTTTGTAGTTGCTACCATTGGTAGCACCCCAATTACAGTTCCTCTTACTGCCAAGTTTCAGCATACTCCAGCTTTTGTGTTAGTCCATAAAATCTCTCTAATTTAAACCTTTCTAACACTTCTGTATCTAAACATTTTCATTGAAAAATTAACTTAATAGTGATAATAATATTATTAGACATTTAATTTTCACCTTATGATTTAATTTGTGGTGAGAATACAGGTACTTTGTGGTAGCCAATGGAATTGTCAGTCTTCATAATTTGGTTATGATAGCAATGGATATATTAGGACTCAAATTTCATAACAAAGGGCTCCAACTTGCACTGATTGCAGTATTTGACACGGTACAAAATAAATGACACCTCTAATTGATCATTTAAGTTAAAATTACTAACATATGAGAACTGATTGATTATATTTTTTTGATTAATTGGGTGAATTAGATGGCTTTGGCTCTAGCATCAAGTGGAGATGGTGCAGCAACATTTATGTCAGAATTGGGAAGGAATGGTAATTCACATGCAAAATGGAATAAAATATGTGACAAATTTGAGTCCTATTGCAACAGAGGTGGTGGTGCCTTAATAGCTTCTTTCATTGGGCTCATTCTCCTACTCATTATTACAGTGATGTCCATCAACAAACTCCTCAAACTAAATCGCAATTAA

Protein sequence

>MS.gene53494.t1

MASGNEEKIESGFNVVPETKPKKDWILLSLRVVAFLATVSATLVMSLNKQTKSFVVATIGSTPITVPLTAKFQHTPAFVYFVVANGIVSLHNLVMIAMDILGLKFHNKGLQLALIAVFDTMALALASSGDGAATFMSELGRNGNSHAKWNKICDKFESYCNRGGGALIASFIGLILLLIITVMSINKLLKLNRN