Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56179.t1 | XP_003588447.3 | 93.6 | 761 | 33 | 2 | 1 | 745 | 1 | 761 | 0.00E+00 | 1425.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56179.t1 | F4HWL4 | 64.3 | 736 | 243 | 6 | 21 | 745 | 22 | 748 | 4.5e-284 | 978.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56179.t1 | A0A445E7G1 | 70.9 | 787 | 167 | 7 | 14 | 739 | 16 | 801 | 0.0e+00 | 1107.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene56179 | 0.815456 | 9.16E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene56179 | 0.811459 | 6.93E-51 | -1.69E-46 |
| MS.gene049382 | MS.gene56179 | 0.804636 | 1.96E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene56179 | 0.837643 | 4.61E-57 | -1.69E-46 |
| MS.gene050226 | MS.gene56179 | 0.845559 | 3.77E-59 | -1.69E-46 |
| MS.gene050227 | MS.gene56179 | 0.846047 | 2.78E-59 | -1.69E-46 |
| MS.gene050228 | MS.gene56179 | 0.850479 | 1.65E-60 | -1.69E-46 |
| MS.gene050438 | MS.gene56179 | 0.819934 | 8.95E-53 | -1.69E-46 |
| MS.gene050442 | MS.gene56179 | 0.823691 | 1.21E-53 | -1.69E-46 |
| MS.gene050516 | MS.gene56179 | 0.816231 | 6.15E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene56179 | 0.829472 | 5.05E-55 | -1.69E-46 |
| MS.gene050586 | MS.gene56179 | 0.808151 | 3.57E-50 | -1.69E-46 |
| MS.gene050623 | MS.gene56179 | -0.832457 | 9.35E-56 | -1.69E-46 |
| MS.gene050719 | MS.gene56179 | 0.826486 | 2.64E-54 | -1.69E-46 |
| MS.gene050721 | MS.gene56179 | 0.8266 | 2.48E-54 | -1.69E-46 |
| MS.gene050724 | MS.gene56179 | 0.817951 | 2.53E-52 | -1.69E-46 |
| MS.gene050762 | MS.gene56179 | 0.818175 | 2.25E-52 | -1.69E-46 |
| MS.gene050763 | MS.gene56179 | 0.820312 | 7.33E-53 | -1.69E-46 |
| MS.gene051626 | MS.gene56179 | 0.820256 | 7.55E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene56179 | 0.824725 | 6.91E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene56179 | 0.842472 | 2.53E-58 | -1.69E-46 |
| MS.gene051860 | MS.gene56179 | 0.839673 | 1.38E-57 | -1.69E-46 |
| MS.gene051983 | MS.gene56179 | 0.801801 | 7.58E-49 | -1.69E-46 |
| MS.gene052020 | MS.gene56179 | 0.806694 | 7.26E-50 | -1.69E-46 |
| MS.gene052256 | MS.gene56179 | 0.819438 | 1.16E-52 | -1.69E-46 |
| MS.gene052323 | MS.gene56179 | 0.801638 | 8.19E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene56179 | 0.842426 | 2.60E-58 | -1.69E-46 |
| MS.gene053630 | MS.gene56179 | 0.823216 | 1.56E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene56179 | 0.841034 | 6.07E-58 | -1.69E-46 |
| MS.gene054184 | MS.gene56179 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene054185 | MS.gene56179 | 0.816449 | 5.50E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene56179 | 0.819992 | 8.68E-53 | -1.69E-46 |
| MS.gene054473 | MS.gene56179 | 0.828354 | 9.42E-55 | -1.69E-46 |
| MS.gene054596 | MS.gene56179 | 0.810321 | 1.22E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene56179 | 0.816207 | 6.23E-52 | -1.69E-46 |
| MS.gene054942 | MS.gene56179 | 0.843824 | 1.10E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene56179 | 0.819864 | 9.28E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene56179 | -0.811119 | 8.21E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene56179 | 0.845391 | 4.18E-59 | -1.69E-46 |
| MS.gene056979 | MS.gene56179 | 0.802397 | 5.72E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene56179 | 0.817502 | 3.19E-52 | -1.69E-46 |
| MS.gene057469 | MS.gene56179 | 0.829567 | 4.79E-55 | -1.69E-46 |
| MS.gene057750 | MS.gene56179 | 0.800555 | 1.36E-48 | -1.69E-46 |
| MS.gene057754 | MS.gene56179 | 0.809508 | 1.83E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene56179 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene56179 | 0.821882 | 3.19E-53 | -1.69E-46 |
| MS.gene058182 | MS.gene56179 | 0.803747 | 3.01E-49 | -1.69E-46 |
| MS.gene058851 | MS.gene56179 | 0.815982 | 6.99E-52 | -1.69E-46 |
| MS.gene058906 | MS.gene56179 | 0.810536 | 1.10E-50 | -1.69E-46 |
| MS.gene058982 | MS.gene56179 | 0.832948 | 7.06E-56 | -1.69E-46 |
| MS.gene059124 | MS.gene56179 | 0.818928 | 1.52E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene56179 | 0.804441 | 2.16E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene56179 | 0.800034 | 1.74E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene56179 | 0.807265 | 5.50E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene56179 | 0.808932 | 2.43E-50 | -1.69E-46 |
| MS.gene060480 | MS.gene56179 | 0.815711 | 8.04E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56179.t1 | MTR_1g007340 | 96.258 | 481 | 18 | 0 | 1 | 481 | 1 | 481 | 0.0 | 956 |
| MS.gene56179.t1 | MTR_1g007340 | 69.231 | 377 | 99 | 3 | 380 | 745 | 2 | 372 | 0.0 | 545 |
| MS.gene56179.t1 | MTR_1g053270 | 81.452 | 372 | 64 | 1 | 6 | 372 | 13 | 384 | 0.0 | 643 |
| MS.gene56179.t1 | MTR_1g053270 | 77.540 | 374 | 69 | 4 | 386 | 745 | 12 | 384 | 0.0 | 600 |
| MS.gene56179.t1 | MTR_1g007325 | 83.133 | 249 | 29 | 2 | 497 | 745 | 1 | 236 | 7.18e-141 | 413 |
| MS.gene56179.t1 | MTR_1g007325 | 65.234 | 256 | 69 | 1 | 117 | 372 | 1 | 236 | 3.34e-114 | 345 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56179.t1 | AT1G28350 | 64.130 | 736 | 244 | 7 | 21 | 745 | 22 | 748 | 0.0 | 986 |
| MS.gene56179.t1 | AT1G28350 | 68.871 | 363 | 110 | 2 | 10 | 372 | 389 | 748 | 1.10e-174 | 519 |
| MS.gene56179.t1 | AT1G28350 | 64.130 | 736 | 244 | 7 | 21 | 745 | 22 | 748 | 0.0 | 986 |
| MS.gene56179.t1 | AT1G28350 | 68.871 | 363 | 110 | 2 | 10 | 372 | 389 | 748 | 1.10e-174 | 519 |
| MS.gene56179.t1 | AT1G28350 | 63.965 | 691 | 229 | 7 | 66 | 745 | 1 | 682 | 0.0 | 923 |
| MS.gene56179.t1 | AT1G28350 | 68.871 | 363 | 110 | 2 | 10 | 372 | 323 | 682 | 1.99e-175 | 519 |
| MS.gene56179.t1 | AT2G33840 | 74.716 | 352 | 87 | 1 | 21 | 372 | 36 | 385 | 0.0 | 568 |
| MS.gene56179.t1 | AT2G33840 | 67.989 | 353 | 102 | 3 | 401 | 745 | 36 | 385 | 7.04e-177 | 511 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8631 | gene | 23252 | 30839 | 23252 | ID=MS.gene56179 |
| 8631 | mRNA | 23252 | 30839 | 23252 | ID=MS.gene56179.t1;Parent=MS.gene56179 |
| 8631 | exon | 23252 | 23310 | 23252 | ID=MS.gene56179.t1.exon1;Parent=MS.gene56179.t1 |
| 8631 | CDS | 23252 | 23310 | 23252 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 23408 | 23558 | 23408 | ID=MS.gene56179.t1.exon2;Parent=MS.gene56179.t1 |
| 8631 | CDS | 23408 | 23558 | 23408 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 23966 | 24255 | 23966 | ID=MS.gene56179.t1.exon3;Parent=MS.gene56179.t1 |
| 8631 | CDS | 23966 | 24255 | 23966 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 24353 | 24449 | 24353 | ID=MS.gene56179.t1.exon4;Parent=MS.gene56179.t1 |
| 8631 | CDS | 24353 | 24449 | 24353 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 24979 | 25084 | 24979 | ID=MS.gene56179.t1.exon5;Parent=MS.gene56179.t1 |
| 8631 | CDS | 24979 | 25084 | 24979 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 25162 | 25244 | 25162 | ID=MS.gene56179.t1.exon6;Parent=MS.gene56179.t1 |
| 8631 | CDS | 25162 | 25244 | 25162 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 25523 | 25668 | 25523 | ID=MS.gene56179.t1.exon7;Parent=MS.gene56179.t1 |
| 8631 | CDS | 25523 | 25668 | 25523 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 25842 | 25947 | 25842 | ID=MS.gene56179.t1.exon8;Parent=MS.gene56179.t1 |
| 8631 | CDS | 25842 | 25947 | 25842 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 26992 | 27051 | 26992 | ID=MS.gene56179.t1.exon9;Parent=MS.gene56179.t1 |
| 8631 | CDS | 26992 | 27051 | 26992 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 27159 | 27175 | 27159 | ID=MS.gene56179.t1.exon10;Parent=MS.gene56179.t1 |
| 8631 | CDS | 27159 | 27175 | 27159 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 27486 | 27578 | 27486 | ID=MS.gene56179.t1.exon11;Parent=MS.gene56179.t1 |
| 8631 | CDS | 27486 | 27578 | 27486 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 27664 | 27805 | 27664 | ID=MS.gene56179.t1.exon12;Parent=MS.gene56179.t1 |
| 8631 | CDS | 27664 | 27805 | 27664 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 28243 | 28532 | 28243 | ID=MS.gene56179.t1.exon13;Parent=MS.gene56179.t1 |
| 8631 | CDS | 28243 | 28532 | 28243 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 28639 | 28714 | 28639 | ID=MS.gene56179.t1.exon14;Parent=MS.gene56179.t1 |
| 8631 | CDS | 28639 | 28714 | 28639 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 29335 | 29440 | 29335 | ID=MS.gene56179.t1.exon15;Parent=MS.gene56179.t1 |
| 8631 | CDS | 29335 | 29440 | 29335 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 29530 | 29612 | 29530 | ID=MS.gene56179.t1.exon16;Parent=MS.gene56179.t1 |
| 8631 | CDS | 29530 | 29612 | 29530 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 29916 | 30061 | 29916 | ID=MS.gene56179.t1.exon17;Parent=MS.gene56179.t1 |
| 8631 | CDS | 29916 | 30061 | 29916 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 30216 | 30321 | 30216 | ID=MS.gene56179.t1.exon18;Parent=MS.gene56179.t1 |
| 8631 | CDS | 30216 | 30321 | 30216 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 30653 | 30712 | 30653 | ID=MS.gene56179.t1.exon19;Parent=MS.gene56179.t1 |
| 8631 | CDS | 30653 | 30712 | 30653 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| 8631 | exon | 30819 | 30839 | 30819 | ID=MS.gene56179.t1.exon20;Parent=MS.gene56179.t1 |
| 8631 | CDS | 30819 | 30839 | 30819 | ID=cds.MS.gene56179.t1;Parent=MS.gene56179.t1 |
| Gene Sequence |
| Protein sequence |
