AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58750.t1 MTR_3g110280 85.794 359 49 1 1 357 1 359 0.0 623
MS.gene58750.t1 MTR_4g116920 86.908 359 32 2 1 358 1 345 0.0 620
MS.gene58750.t1 MTR_3g110320 80.992 363 68 1 1 362 1 363 0.0 607
MS.gene58750.t1 MTR_3g110320 81.058 359 67 1 1 358 1 359 0.0 600
MS.gene58750.t1 MTR_1g013150 37.079 356 204 6 22 362 32 382 1.61e-69 223
MS.gene58750.t1 MTR_4g117000 35.838 346 207 7 30 363 35 377 7.51e-64 208
MS.gene58750.t1 MTR_4g116990 38.375 357 187 9 30 363 37 383 2.36e-62 204
MS.gene58750.t1 MTR_4g117020 35.013 377 218 10 3 363 2 367 2.03e-61 210
MS.gene58750.t1 MTR_4g117040 37.465 355 196 9 26 363 24 369 2.90e-61 210
MS.gene58750.t1 MTR_4g117030 36.499 337 189 9 41 361 1 328 1.16e-58 202
MS.gene58750.t1 MTR_4g117800 36.364 176 107 2 6 179 4 176 1.71e-24 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58750.t1 AT4G19810 51.913 366 158 7 11 364 9 368 4.47e-119 350
MS.gene58750.t1 AT4G19810 51.913 366 158 7 11 364 9 368 4.47e-119 350
MS.gene58750.t1 AT4G19820 46.978 364 181 6 10 364 6 366 2.11e-102 306
MS.gene58750.t1 AT4G19760 46.092 371 171 10 17 363 1 366 2.63e-95 288
MS.gene58750.t1 AT4G19750 45.658 357 175 9 17 359 1 352 1.82e-93 283
MS.gene58750.t1 AT4G19800 45.882 340 171 6 29 359 6 341 1.28e-92 283
MS.gene58750.t1 AT4G19720 46.319 326 162 6 46 364 33 352 1.65e-89 273
MS.gene58750.t1 AT4G19730 41.593 339 166 8 17 330 1 332 6.40e-71 225
MS.gene58750.t1 AT4G19770 41.538 260 142 4 112 363 1 258 5.39e-58 189
MS.gene58750.t1 AT4G19740 37.173 191 106 5 135 315 24 210 1.80e-29 113

Find 95 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCAATAGCCACCTTTAA+TGG 0.214652 8.1:-10200898 None:intergenic
GTTACTAATTATTGCTTCAT+AGG 0.237729 8.1:+10201731 MS.gene58750:CDS
TCCCACCACCAATTGATAAT+AGG 0.244910 8.1:-10201094 None:intergenic
GCCAAGCAAAATGGTTTGTT+TGG 0.270489 8.1:+10201809 MS.gene58750:CDS
CCCACCACCAATTGATAATA+GGG 0.272819 8.1:-10201093 None:intergenic
ACCACAAACGCTGCCATTAA+AGG 0.289713 8.1:+10200885 MS.gene58750:CDS
GTTGATTATGCCAAGCAAAA+TGG 0.313698 8.1:+10201800 MS.gene58750:CDS
ATCAAGCTACACAGGGGCTT+GGG 0.319160 8.1:+10201648 MS.gene58750:CDS
CAACTTTGGTTTACTCATTA+CGG 0.336590 8.1:+10201268 MS.gene58750:CDS
ACCTTTAATGGCAGCGTTTG+TGG 0.356603 8.1:-10200886 None:intergenic
GCCGAAGAGTCTAGCAGTTC+TGG 0.357634 8.1:+10201308 MS.gene58750:CDS
ATATTGAACAAGACAGCAAC+TGG 0.377045 8.1:+10201846 MS.gene58750:CDS
ACAAAGATATAATAAATGTT+GGG 0.382597 8.1:+10201681 MS.gene58750:CDS
CAGCGTTTGTGGTGGATAAC+TGG 0.392336 8.1:-10200875 None:intergenic
CTGCCATTAAAGGTGGCTAT+TGG 0.398685 8.1:+10200895 MS.gene58750:CDS
TACCTTCATCTACACTGAAT+TGG 0.403692 8.1:-10201517 None:intergenic
TAGATGAAGGTATCACAAAA+TGG 0.403845 8.1:+10201528 MS.gene58750:CDS
GATCAAGCTACACAGGGGCT+TGG 0.408064 8.1:+10201647 MS.gene58750:CDS
GTGAAAACCCTATTATCAAT+TGG 0.411073 8.1:+10201086 MS.gene58750:CDS
CTCTGATCAGATCACCCCTT+TGG 0.414677 8.1:+10201367 MS.gene58750:CDS
TCGATGGTCTTGATCTTGAC+TGG 0.423357 8.1:+10201216 MS.gene58750:CDS
ATTGCTTCATAGGGACAGAT+TGG 0.433223 8.1:+10201741 MS.gene58750:CDS
GTCTTATCCGCGTCGGAGGA+TGG 0.434776 8.1:-10201245 None:intergenic
GAAGGTATCACAAAATGGAT+TGG 0.445585 8.1:+10201533 MS.gene58750:CDS
TTACTAATTATTGCTTCATA+GGG 0.446458 8.1:+10201732 MS.gene58750:CDS
GACGCGGATAAGACCAACTT+TGG 0.447081 8.1:+10201254 MS.gene58750:CDS
ATCACAAAATGGATTGGGTT+AGG 0.453409 8.1:+10201539 MS.gene58750:CDS
TACAAAGATATAATAAATGT+TGG 0.455760 8.1:+10201680 MS.gene58750:CDS
TATAATAAATGTTGGGGCGC+AGG 0.456722 8.1:+10201688 MS.gene58750:CDS
GAACAAGTGGGTGAAATAAG+AGG 0.458120 8.1:-10200955 None:intergenic
ACGACCTTTACATGTCAGAT+AGG 0.459436 8.1:+10201447 MS.gene58750:CDS
ATATTGCAAACAACTTGGAT+TGG 0.476015 8.1:+10201408 MS.gene58750:CDS
AAGGTATCACAAAATGGATT+GGG 0.479422 8.1:+10201534 MS.gene58750:CDS
TTCTGCAGCTTCTCAAGCAT+GGG 0.481382 8.1:+10202438 MS.gene58750:intron
TATTGCAAACAACTTGGATT+GGG 0.481756 8.1:+10201409 MS.gene58750:CDS
GTTGGTTGGTATTAGAATCA+AGG 0.482451 8.1:-10200992 None:intergenic
CGTAATGAGTAAACCAAAGT+TGG 0.491887 8.1:-10201267 None:intergenic
GTTATTTCTAGCAAGTTGAA+TGG 0.492439 8.1:-10201189 None:intergenic
TCAGGATATTGCAAACAACT+TGG 0.496362 8.1:+10201403 MS.gene58750:CDS
CGATGGTCTTGATCTTGACT+GGG 0.498899 8.1:+10201217 MS.gene58750:CDS
TTTCTGCAGCTTCTCAAGCA+TGG 0.499088 8.1:+10202437 MS.gene58750:intron
GCATTGGGTGTACCTTTCTA+CGG 0.499133 8.1:+10201581 MS.gene58750:CDS
AACCTTCACTGAAACCGTTC+AGG 0.501026 8.1:+10201049 MS.gene58750:CDS
TGGTGGTGGGAATTCAACTT+TGG 0.502902 8.1:+10201106 MS.gene58750:CDS
TGCAGCTTCTCAAGCATGGG+AGG 0.503853 8.1:+10202441 MS.gene58750:intron
ACCCTATTATCAATTGGTGG+TGG 0.504311 8.1:+10201092 MS.gene58750:CDS
TGCCTGAACGGTTTCAGTGA+AGG 0.504697 8.1:-10201051 None:intergenic
GCCAGAACTGCTAGACTCTT+CGG 0.504881 8.1:-10201309 None:intergenic
GATACCTATCTGACATGTAA+AGG 0.511041 8.1:-10201451 None:intergenic
AACTGGGCTCTTTCTCAAGC+AGG 0.514071 8.1:+10201863 MS.gene58750:CDS
TTGGAGAATCATGAGGAGAG+TGG 0.515988 8.1:-10200850 None:intergenic
GGCTATTGGAATTCTGATAG+TGG 0.524115 8.1:+10200909 MS.gene58750:CDS
TACCTTTCTACGGTTTCAAG+TGG 0.524260 8.1:+10201591 MS.gene58750:CDS
GCAATAATTAGTAACATATG+TGG 0.528980 8.1:-10201723 None:intergenic
TCTACACTGAATTGGCCTGT+TGG 0.531450 8.1:-10201509 None:intergenic
CCCTATTATCAATTGGTGGT+GGG 0.533806 8.1:+10201093 MS.gene58750:CDS
GACCTGGGTAGTATTCCAAA+GGG 0.538829 8.1:-10201382 None:intergenic
CTCCACTTGAAACCGTAGAA+AGG 0.540691 8.1:-10201593 None:intergenic
CGTTCAGGCAAAGAGTAGTT+CGG 0.545594 8.1:+10201064 MS.gene58750:CDS
TTGTTTGCAATATCCTGACC+TGG 0.547851 8.1:-10201398 None:intergenic
TTGTTATCTGCTGCAGTGGG+TGG 0.559981 8.1:+10201344 MS.gene58750:CDS
CGAACTACTCTTTGCCTGAA+CGG 0.560291 8.1:-10201063 None:intergenic
CAGCAAATGCACAGAACAAG+TGG 0.562193 8.1:-10200968 None:intergenic
TTGCTTGGCATAATCAACCT+TGG 0.564771 8.1:-10201795 None:intergenic
TTTGGAATACTACCCAGGTC+AGG 0.567695 8.1:+10201385 MS.gene58750:CDS
GTTGGTCTTATCCGCGTCGG+AGG 0.568312 8.1:-10201249 None:intergenic
TCAAGCTACACAGGGGCTTG+GGG 0.574229 8.1:+10201649 MS.gene58750:CDS
AACAAGTGGGTGAAATAAGA+GGG 0.575691 8.1:-10200954 None:intergenic
TACGGAGTGGAGAGCTGCTG+TGG 0.585644 8.1:+10201286 MS.gene58750:CDS
GGCCAATTCAGTGTAGATGA+AGG 0.587139 8.1:+10201515 MS.gene58750:CDS
TTGTGGTGGATAACTGGAGT+TGG 0.587390 8.1:-10200869 None:intergenic
CACTCAGAGTATATCTGCCA+AGG 0.587655 8.1:+10201778 MS.gene58750:CDS
TGACCTGGGTAGTATTCCAA+AGG 0.587785 8.1:-10201383 None:intergenic
CAAAGATATAATAAATGTTG+GGG 0.588056 8.1:+10201682 MS.gene58750:CDS
AAAACCCTATTATCAATTGG+TGG 0.588303 8.1:+10201089 MS.gene58750:CDS
TTTAATGGCAGCGTTTGTGG+TGG 0.594547 8.1:-10200883 None:intergenic
ACCCCTTTGGAATACTACCC+AGG 0.599496 8.1:+10201380 MS.gene58750:CDS
AGAAGAAATAGTGAGTTGGT+TGG 0.600058 8.1:-10201006 None:intergenic
CTGTTGTTATCTGCTGCAGT+GGG 0.603516 8.1:+10201341 MS.gene58750:CDS
TGAGGAGAGTGGAAATCAAG+AGG 0.606133 8.1:-10200839 None:intergenic
TTCATAGGGACAGATTGGTA+TGG 0.610823 8.1:+10201746 MS.gene58750:CDS
ACTGTTGTTATCTGCTGCAG+TGG 0.613628 8.1:+10201340 MS.gene58750:CDS
GCTAGAAATAACAACTTCGA+TGG 0.620728 8.1:+10201200 MS.gene58750:CDS
TTGTAGAAGAAATAGTGAGT+TGG 0.631938 8.1:-10201010 None:intergenic
GCAGCAGATAACAACAGTGT+CGG 0.646329 8.1:-10201335 None:intergenic
GATCAATGTCAGAAACTGCA+AGG 0.646791 8.1:-10200932 None:intergenic
TGTTTGCAATATCCTGACCT+GGG 0.647942 8.1:-10201397 None:intergenic
TTGGTTTACTCATTACGGAG+TGG 0.651665 8.1:+10201273 MS.gene58750:CDS
AAAGTTGGTCTTATCCGCGT+CGG 0.662235 8.1:-10201252 None:intergenic
ACTGGAGTTGGAGAATCATG+AGG 0.662538 8.1:-10200857 None:intergenic
ACAAACGCTGCCATTAAAGG+TGG 0.669351 8.1:+10200888 MS.gene58750:CDS
TATTGAACAAGACAGCAACT+GGG 0.675280 8.1:+10201847 MS.gene58750:CDS
AGCAAATGCACAGAACAAGT+GGG 0.684809 8.1:-10200967 None:intergenic
ACCTGGGTAGTATTCCAAAG+GGG 0.736054 8.1:-10201381 None:intergenic
GGAGTATCCATCCTCCGACG+CGG 0.742661 8.1:+10201238 MS.gene58750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAGATATAATAAATGTT+GGG + chr8.1:10201681-10201700 MS.gene58750:CDS 15.0%
!! CTACTTATTATATAATCATA+CGG + chr8.1:10201924-10201943 MS.gene58750:intron 15.0%
!! TACAAAGATATAATAAATGT+TGG + chr8.1:10201680-10201699 MS.gene58750:CDS 15.0%
!! TGATTATATAATAAGTAGAA+CGG - chr8.1:10201922-10201941 None:intergenic 15.0%
!! TTTAGACCTATATATTTATT+CGG + chr8.1:10202264-10202283 MS.gene58750:intron 15.0%
!!! TTGTTTGTTTGTTTTTTATA+TGG + chr8.1:10201953-10201972 MS.gene58750:intron 15.0%
!! CAAAGATATAATAAATGTTG+GGG + chr8.1:10201682-10201701 MS.gene58750:CDS 20.0%
!! TCAAAAATTTCATGGTTATT+AGG - chr8.1:10201629-10201648 None:intergenic 20.0%
!! TTACTAATTATTGCTTCATA+GGG + chr8.1:10201732-10201751 MS.gene58750:CDS 20.0%
!!! CTATCTATCTTTTTTGATAT+GGG - chr8.1:10202325-10202344 None:intergenic 20.0%
!!! TCTATCTATCTTTTTTGATA+TGG - chr8.1:10202326-10202345 None:intergenic 20.0%
! GCAATAATTAGTAACATATG+TGG - chr8.1:10201726-10201745 None:intergenic 25.0%
! GTTACTAATTATTGCTTCAT+AGG + chr8.1:10201731-10201750 MS.gene58750:CDS 25.0%
! TAGCTTGATCAAAAATTTCA+TGG - chr8.1:10201637-10201656 None:intergenic 25.0%
!! AAGAATCTATGAACGATTTT+CGG - chr8.1:10201169-10201188 None:intergenic 25.0%
!! ATATGGCTAACTTCAAATTT+TGG + chr8.1:10201970-10201989 MS.gene58750:intron 25.0%
!!! CTAACTTCAAATTTTGGTTT+AGG + chr8.1:10201976-10201995 MS.gene58750:intron 25.0%
AAAACCCTATTATCAATTGG+TGG + chr8.1:10201089-10201108 MS.gene58750:CDS 30.0%
ATATTGCAAACAACTTGGAT+TGG + chr8.1:10201408-10201427 MS.gene58750:CDS 30.0%
ATCAGTAGTATATCACCATT+CGG + chr8.1:10202090-10202109 MS.gene58750:intron 30.0%
GAAATGGCATTAAGAATAAG+AGG - chr8.1:10202371-10202390 None:intergenic 30.0%
GAATAAGAGGTAAAACTCTA+AGG - chr8.1:10202358-10202377 None:intergenic 30.0%
GTGAAAACCCTATTATCAAT+TGG + chr8.1:10201086-10201105 MS.gene58750:CDS 30.0%
TATTGCAAACAACTTGGATT+GGG + chr8.1:10201409-10201428 MS.gene58750:CDS 30.0%
TTGTAGAAGAAATAGTGAGT+TGG - chr8.1:10201013-10201032 None:intergenic 30.0%
! AAGGTATCACAAAATGGATT+GGG + chr8.1:10201534-10201553 MS.gene58750:CDS 30.0%
! AATTTTTGATCAAGCTACAC+AGG + chr8.1:10201640-10201659 MS.gene58750:CDS 30.0%
! ACCGGTTTAACCAATTTAAA+AGG - chr8.1:10202119-10202138 None:intergenic 30.0%
! ATTTTTGATCAAGCTACACA+GGG + chr8.1:10201641-10201660 MS.gene58750:CDS 30.0%
! CAACTTTGGTTTACTCATTA+CGG + chr8.1:10201268-10201287 MS.gene58750:CDS 30.0%
! GCCTTTTAAATTGGTTAAAC+CGG + chr8.1:10202115-10202134 MS.gene58750:intron 30.0%
! GTTTGTTTGGATATTTTGCT+TGG + chr8.1:10201822-10201841 MS.gene58750:CDS 30.0%
!! GGCAAAAAAATTTGCTGATA+TGG + chr8.1:10201127-10201146 MS.gene58750:CDS 30.0%
!! GTTATTTCTAGCAAGTTGAA+TGG - chr8.1:10201192-10201211 None:intergenic 30.0%
!! TAGATGAAGGTATCACAAAA+TGG + chr8.1:10201528-10201547 MS.gene58750:CDS 30.0%
AACAAGTGGGTGAAATAAGA+GGG - chr8.1:10200957-10200976 None:intergenic 35.0%
AGCGGTAATTAACTACTGAT+GGG + chr8.1:10202152-10202171 MS.gene58750:intron 35.0%
ATATTGAACAAGACAGCAAC+TGG + chr8.1:10201846-10201865 MS.gene58750:CDS 35.0%
ATCATAGCGAGTCATTCTAA+TGG + chr8.1:10202398-10202417 MS.gene58750:intron 35.0%
ATTCCAATAGCCACCTTTAA+TGG - chr8.1:10200901-10200920 None:intergenic 35.0%
CCAAAAAGCAAACTAGCATT+GGG + chr8.1:10201566-10201585 MS.gene58750:CDS 35.0%
CGCTATGATACAGTTTGAAA+TGG - chr8.1:10202387-10202406 None:intergenic 35.0%
CGTAATGAGTAAACCAAAGT+TGG - chr8.1:10201270-10201289 None:intergenic 35.0%
GAACGGAGCTATGTTTAATT+AGG - chr8.1:10201905-10201924 None:intergenic 35.0%
GAATGGTGATATACTACTGA+TGG - chr8.1:10202091-10202110 None:intergenic 35.0%
GATACCTATCTGACATGTAA+AGG - chr8.1:10201454-10201473 None:intergenic 35.0%
GCTAGAAATAACAACTTCGA+TGG + chr8.1:10201200-10201219 MS.gene58750:CDS 35.0%
GGTTTAGGCCTATATTTGTT+GGG + chr8.1:10201991-10202010 MS.gene58750:intron 35.0%
GTTGATTATGCCAAGCAAAA+TGG + chr8.1:10201800-10201819 MS.gene58750:CDS 35.0%
TACCTTCATCTACACTGAAT+TGG - chr8.1:10201520-10201539 None:intergenic 35.0%
TATTGAACAAGACAGCAACT+GGG + chr8.1:10201847-10201866 MS.gene58750:CDS 35.0%
TCAGGATATTGCAAACAACT+TGG + chr8.1:10201403-10201422 MS.gene58750:CDS 35.0%
TCCAAAAAGCAAACTAGCAT+TGG + chr8.1:10201565-10201584 MS.gene58750:CDS 35.0%
TGGTTTAGGCCTATATTTGT+TGG + chr8.1:10201990-10202009 MS.gene58750:intron 35.0%
! AATTAACTACCGTTCGAGTT+AGG + chr8.1:10202186-10202205 MS.gene58750:intron 35.0%
! CAAGAGGAATGAATGCTTTT+TGG - chr8.1:10200826-10200845 None:intergenic 35.0%
! CATTCGGATGCCTTTTAAAT+TGG + chr8.1:10202106-10202125 MS.gene58750:intron 35.0%
! GTTGGTTGGTATTAGAATCA+AGG - chr8.1:10200995-10201014 None:intergenic 35.0%
! TCCAAACAAACCATTTTGCT+TGG - chr8.1:10201813-10201832 None:intergenic 35.0%
! TTTTTGATCAAGCTACACAG+GGG + chr8.1:10201642-10201661 MS.gene58750:CDS 35.0%
!! AAAAGTATCCCTTGGGAATA+GGG - chr8.1:10202294-10202313 None:intergenic 35.0%
!! AATTGACCAAAAGTATCCCT+TGG - chr8.1:10202302-10202321 None:intergenic 35.0%
!! AGAAGAAATAGTGAGTTGGT+TGG - chr8.1:10201009-10201028 None:intergenic 35.0%
!! ATCACAAAATGGATTGGGTT+AGG + chr8.1:10201539-10201558 MS.gene58750:CDS 35.0%
!! ATTGACCAAAAGTATCCCTT+GGG - chr8.1:10202301-10202320 None:intergenic 35.0%
!! CAATTTAAAAGGCATCCGAA+TGG - chr8.1:10202108-10202127 None:intergenic 35.0%
!! GAAGGTATCACAAAATGGAT+TGG + chr8.1:10201533-10201552 MS.gene58750:CDS 35.0%
ACCCTATTATCAATTGGTGG+TGG + chr8.1:10201092-10201111 MS.gene58750:CDS 40.0%
ACGACCTTTACATGTCAGAT+AGG + chr8.1:10201447-10201466 MS.gene58750:CDS 40.0%
AGCAAATGCACAGAACAAGT+GGG - chr8.1:10200970-10200989 None:intergenic 40.0%
ATCCCAAGAGATTGCTCTTT+AGG - chr8.1:10202235-10202254 None:intergenic 40.0%
ATTGCTTCATAGGGACAGAT+TGG + chr8.1:10201741-10201760 MS.gene58750:CDS 40.0%
CCGGTTTAAAAAGTGATGAG+CGG + chr8.1:10202134-10202153 MS.gene58750:intron 40.0%
GAACAAGTGGGTGAAATAAG+AGG - chr8.1:10200958-10200977 None:intergenic 40.0%
GAGCGGTAATTAACTACTGA+TGG + chr8.1:10202151-10202170 MS.gene58750:intron 40.0%
GATCAATGTCAGAAACTGCA+AGG - chr8.1:10200935-10200954 None:intergenic 40.0%
GCGGTAATTAACTACTGATG+GGG + chr8.1:10202153-10202172 MS.gene58750:intron 40.0%
GGCTATTGGAATTCTGATAG+TGG + chr8.1:10200909-10200928 MS.gene58750:CDS 40.0%
GGTCGGCCGAATAAATATAT+AGG - chr8.1:10202273-10202292 None:intergenic 40.0%
TACCTTTCTACGGTTTCAAG+TGG + chr8.1:10201591-10201610 MS.gene58750:CDS 40.0%
TATAATAAATGTTGGGGCGC+AGG + chr8.1:10201688-10201707 MS.gene58750:CDS 40.0%
TCCTTAATCCCATGAGGATA+GGG - chr8.1:10202021-10202040 None:intergenic 40.0%
TGCCTAAAGAGCAATCTCTT+GGG + chr8.1:10202230-10202249 MS.gene58750:intron 40.0%
TGGTGATATACTACTGATGG+CGG - chr8.1:10202088-10202107 None:intergenic 40.0%
TGTTTGCAATATCCTGACCT+GGG - chr8.1:10201400-10201419 None:intergenic 40.0%
TTCATAGGGACAGATTGGTA+TGG + chr8.1:10201746-10201765 MS.gene58750:CDS 40.0%
TTCCTTAATCCCATGAGGAT+AGG - chr8.1:10202022-10202041 None:intergenic 40.0%
TTGCTTGGCATAATCAACCT+TGG - chr8.1:10201798-10201817 None:intergenic 40.0%
TTGGTTTACTCATTACGGAG+TGG + chr8.1:10201273-10201292 MS.gene58750:CDS 40.0%
TTGTTTGCAATATCCTGACC+TGG - chr8.1:10201401-10201420 None:intergenic 40.0%
! CCCACCACCAATTGATAATA+GGG - chr8.1:10201096-10201115 None:intergenic 40.0%
! CCCTATTATCAATTGGTGGT+GGG + chr8.1:10201093-10201112 MS.gene58750:CDS 40.0%
! CTATTCCCAAGGGATACTTT+TGG + chr8.1:10202293-10202312 MS.gene58750:intron 40.0%
! GCTCCTTTGAAAAATCCAAC+AGG + chr8.1:10201494-10201513 MS.gene58750:CDS 40.0%
! TATGAACGATTTTCGGCTAC+TGG - chr8.1:10201162-10201181 None:intergenic 40.0%
! TCCCACCACCAATTGATAAT+AGG - chr8.1:10201097-10201116 None:intergenic 40.0%
!! CAAAAGTATCCCTTGGGAAT+AGG - chr8.1:10202295-10202314 None:intergenic 40.0%
!! GCCAAGCAAAATGGTTTGTT+TGG + chr8.1:10201809-10201828 MS.gene58750:CDS 40.0%
!! GTTGGATTTTTCAAAGGAGC+AGG - chr8.1:10201494-10201513 None:intergenic 40.0%
!! TGGCCTGTTGGATTTTTCAA+AGG - chr8.1:10201500-10201519 None:intergenic 40.0%
!!! CCCAATGCTAGTTTGCTTTT+TGG - chr8.1:10201569-10201588 None:intergenic 40.0%
!!! CCGCTCATCACTTTTTAAAC+CGG - chr8.1:10202137-10202156 None:intergenic 40.0%
AACCTTCACTGAAACCGTTC+AGG + chr8.1:10201049-10201068 MS.gene58750:CDS 45.0%
ACAAACGCTGCCATTAAAGG+TGG + chr8.1:10200888-10200907 MS.gene58750:CDS 45.0%
ACCACAAACGCTGCCATTAA+AGG + chr8.1:10200885-10200904 MS.gene58750:CDS 45.0%
ACCCTATCCTCATGGGATTA+AGG + chr8.1:10202017-10202036 MS.gene58750:intron 45.0%
ACCTGGGTAGTATTCCAAAG+GGG - chr8.1:10201384-10201403 None:intergenic 45.0%
ACCTTTAATGGCAGCGTTTG+TGG - chr8.1:10200889-10200908 None:intergenic 45.0%
ACTGGAGTTGGAGAATCATG+AGG - chr8.1:10200860-10200879 None:intergenic 45.0%
ACTGTTGTTATCTGCTGCAG+TGG + chr8.1:10201340-10201359 MS.gene58750:CDS 45.0%
AGGGTCAGCCCAACAAATAT+AGG - chr8.1:10202002-10202021 None:intergenic 45.0%
CACTCAGAGTATATCTGCCA+AGG + chr8.1:10201778-10201797 MS.gene58750:CDS 45.0%
CAGCAAATGCACAGAACAAG+TGG - chr8.1:10200971-10200990 None:intergenic 45.0%
CGAACTACTCTTTGCCTGAA+CGG - chr8.1:10201066-10201085 None:intergenic 45.0%
CGTTCAGGCAAAGAGTAGTT+CGG + chr8.1:10201064-10201083 MS.gene58750:CDS 45.0%
CTCCACTTGAAACCGTAGAA+AGG - chr8.1:10201596-10201615 None:intergenic 45.0%
CTGCCATTAAAGGTGGCTAT+TGG + chr8.1:10200895-10200914 MS.gene58750:CDS 45.0%
CTGTTGTTATCTGCTGCAGT+GGG + chr8.1:10201341-10201360 MS.gene58750:CDS 45.0%
GACCTGGGTAGTATTCCAAA+GGG - chr8.1:10201385-10201404 None:intergenic 45.0%
GCAACTTCCTTAATCCCATG+AGG - chr8.1:10202027-10202046 None:intergenic 45.0%
GCAGCAGATAACAACAGTGT+CGG - chr8.1:10201338-10201357 None:intergenic 45.0%
GCATTGGGTGTACCTTTCTA+CGG + chr8.1:10201581-10201600 MS.gene58750:CDS 45.0%
GGCCAATTCAGTGTAGATGA+AGG + chr8.1:10201515-10201534 MS.gene58750:CDS 45.0%
GGTGATATACTACTGATGGC+GGG - chr8.1:10202087-10202106 None:intergenic 45.0%
GTGCCTAAAGAGCAATCTCT+TGG + chr8.1:10202229-10202248 MS.gene58750:intron 45.0%
TCACATGCACCTAACTCGAA+CGG - chr8.1:10202198-10202217 None:intergenic 45.0%
TGACCTGGGTAGTATTCCAA+AGG - chr8.1:10201386-10201405 None:intergenic 45.0%
TGAGGAGAGTGGAAATCAAG+AGG - chr8.1:10200842-10200861 None:intergenic 45.0%
TTCGCCTAGCAATTTCTCAC+AGG + chr8.1:10202049-10202068 MS.gene58750:intron 45.0%
TTCTGCAGCTTCTCAAGCAT+GGG + chr8.1:10202438-10202457 MS.gene58750:intron 45.0%
TTGGAGAATCATGAGGAGAG+TGG - chr8.1:10200853-10200872 None:intergenic 45.0%
TTTAATGGCAGCGTTTGTGG+TGG - chr8.1:10200886-10200905 None:intergenic 45.0%
TTTCTGCAGCTTCTCAAGCA+TGG + chr8.1:10202437-10202456 MS.gene58750:intron 45.0%
! CGATGGTCTTGATCTTGACT+GGG + chr8.1:10201217-10201236 MS.gene58750:CDS 45.0%
! GTGATATACTACTGATGGCG+GGG - chr8.1:10202086-10202105 None:intergenic 45.0%
! TCGATGGTCTTGATCTTGAC+TGG + chr8.1:10201216-10201235 MS.gene58750:CDS 45.0%
! TCTACACTGAATTGGCCTGT+TGG - chr8.1:10201512-10201531 None:intergenic 45.0%
! TTTGGAATACTACCCAGGTC+AGG + chr8.1:10201385-10201404 MS.gene58750:CDS 45.0%
!! AAAGTTGGTCTTATCCGCGT+CGG - chr8.1:10201255-10201274 None:intergenic 45.0%
!! GGATTTTTCAAAGGAGCAGG+TGG - chr8.1:10201491-10201510 None:intergenic 45.0%
!! TGGTGGTGGGAATTCAACTT+TGG + chr8.1:10201106-10201125 MS.gene58750:CDS 45.0%
!! TTGTGGTGGATAACTGGAGT+TGG - chr8.1:10200872-10200891 None:intergenic 45.0%
AACTGGGCTCTTTCTCAAGC+AGG + chr8.1:10201863-10201882 MS.gene58750:CDS 50.0%
ATCAAGCTACACAGGGGCTT+GGG + chr8.1:10201648-10201667 MS.gene58750:CDS 50.0%
CTCTGATCAGATCACCCCTT+TGG + chr8.1:10201367-10201386 MS.gene58750:CDS 50.0%
CTCTTTCTCAAGCAGGTAGG+TGG + chr8.1:10201870-10201889 MS.gene58750:intron 50.0%
GACGCGGATAAGACCAACTT+TGG + chr8.1:10201254-10201273 MS.gene58750:CDS 50.0%
GCCAGAACTGCTAGACTCTT+CGG - chr8.1:10201312-10201331 None:intergenic 50.0%
GGTGCATGTGAGCAATTGCT+AGG + chr8.1:10202207-10202226 MS.gene58750:intron 50.0%
GGTGCCTGTGAGAAATTGCT+AGG - chr8.1:10202056-10202075 None:intergenic 50.0%
GTATCCCTTGGGAATAGGGT+CGG - chr8.1:10202290-10202309 None:intergenic 50.0%
TGCCTGAACGGTTTCAGTGA+AGG - chr8.1:10201054-10201073 None:intergenic 50.0%
TTGTTATCTGCTGCAGTGGG+TGG + chr8.1:10201344-10201363 MS.gene58750:CDS 50.0%
!! ACCCCTTTGGAATACTACCC+AGG + chr8.1:10201380-10201399 MS.gene58750:CDS 50.0%
!! ACGATTTTCGGCTACTGGCT+TGG - chr8.1:10201157-10201176 None:intergenic 50.0%
!! CAGCGTTTGTGGTGGATAAC+TGG - chr8.1:10200878-10200897 None:intergenic 50.0%
GATCAAGCTACACAGGGGCT+TGG + chr8.1:10201647-10201666 MS.gene58750:CDS 55.0%
GCCGAAGAGTCTAGCAGTTC+TGG + chr8.1:10201308-10201327 MS.gene58750:CDS 55.0%
GGGCTCTTTCTCAAGCAGGT+AGG + chr8.1:10201867-10201886 MS.gene58750:intron 55.0%
TCAAGCTACACAGGGGCTTG+GGG + chr8.1:10201649-10201668 MS.gene58750:CDS 55.0%
TGCAGCTTCTCAAGCATGGG+AGG + chr8.1:10202441-10202460 MS.gene58750:intron 55.0%
TGGGCTGACCCTATCCTCAT+GGG + chr8.1:10202010-10202029 MS.gene58750:intron 55.0%
TTGGGCTGACCCTATCCTCA+TGG + chr8.1:10202009-10202028 MS.gene58750:intron 55.0%
!!! TACTGATGGCGGGGCGTTTT+AGG - chr8.1:10202077-10202096 None:intergenic 55.0%
GTCTTATCCGCGTCGGAGGA+TGG - chr8.1:10201248-10201267 None:intergenic 60.0%
TACGGAGTGGAGAGCTGCTG+TGG + chr8.1:10201286-10201305 MS.gene58750:CDS 60.0%
TCGGCCGACCCTATTCCCAA+GGG + chr8.1:10202283-10202302 MS.gene58750:intron 60.0%
TTCGGCCGACCCTATTCCCA+AGG + chr8.1:10202282-10202301 MS.gene58750:intron 60.0%
! GGAGTATCCATCCTCCGACG+CGG + chr8.1:10201238-10201257 MS.gene58750:CDS 60.0%
!! GTTGGTCTTATCCGCGTCGG+AGG - chr8.1:10201252-10201271 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 10200813 10202468 10200813 ID=MS.gene58750
chr8.1 mRNA 10200813 10202468 10200813 ID=MS.gene58750.t1;Parent=MS.gene58750
chr8.1 exon 10200813 10201884 10200813 ID=MS.gene58750.t1.exon1;Parent=MS.gene58750.t1
chr8.1 CDS 10200813 10201884 10200813 ID=cds.MS.gene58750.t1;Parent=MS.gene58750.t1
chr8.1 exon 10202446 10202468 10202446 ID=MS.gene58750.t1.exon2;Parent=MS.gene58750.t1
chr8.1 CDS 10202446 10202468 10202446 ID=cds.MS.gene58750.t1;Parent=MS.gene58750.t1
Gene Sequence

>MS.gene58750

ATGGCTTACTCCAAAAAGCATTCATTCCTCTTGATTTCCACTCTCCTCATGATTCTCCAACTCCAGTTATCCACCACAAACGCTGCCATTAAAGGTGGCTATTGGAATTCTGATAGTGGCCTTGCAGTTTCTGACATTGATCCCTCTTATTTCACCCACTTGTTCTGTGCATTTGCTGACCTTGATTCTAATACCAACCAACTCACTATTTCTTCTACAAACACAGCTAGTTTCTCAACCTTCACTGAAACCGTTCAGGCAAAGAGTAGTTCGGTGAAAACCCTATTATCAATTGGTGGTGGGAATTCAACTTTGGCAAAAAAATTTGCTGATATGGCTAGCCAAGCCAGTAGCCGAAAATCGTTCATAGATTCTTCCATTCAACTTGCTAGAAATAACAACTTCGATGGTCTTGATCTTGACTGGGAGTATCCATCCTCCGACGCGGATAAGACCAACTTTGGTTTACTCATTACGGAGTGGAGAGCTGCTGTGGCCGAAGAGTCTAGCAGTTCTGGCAAGCCGACACTGTTGTTATCTGCTGCAGTGGGTGGCTCTGATCAGATCACCCCTTTGGAATACTACCCAGGTCAGGATATTGCAAACAACTTGGATTGGGTCAATGTAATGACTTACGACCTTTACATGTCAGATAGGTATCAAACTCAGACACAACCACCTGCTCCTTTGAAAAATCCAACAGGCCAATTCAGTGTAGATGAAGGTATCACAAAATGGATTGGGTTAGGAGTTCCAAAAAGCAAACTAGCATTGGGTGTACCTTTCTACGGTTTCAAGTGGAGTTTGTCAGATCCTAATAACCATGAAATTTTTGATCAAGCTACACAGGGGCTTGGGGCAGTGAAGTACAAAGATATAATAAATGTTGGGGCGCAGGTTGTGTACAATTCCACATATGTTACTAATTATTGCTTCATAGGGACAGATTGGTATGGATACGATGACACTCAGAGTATATCTGCCAAGGTTGATTATGCCAAGCAAAATGGTTTGTTTGGATATTTTGCTTGGAATATTGAACAAGACAGCAACTGGGCTCTTTCTCAAGCAGGTAGGTGGATTAGTTCTCCTAATTAAACATAGCTCCGTTCTACTTATTATATAATCATACGGAGTTTGTTGTTTGTTTGTTTTTTATATGGCTAACTTCAAATTTTGGTTTAGGCCTATATTTGTTGGGCTGACCCTATCCTCATGGGATTAAGGAAGTTGCTCTTCGCCTAGCAATTTCTCACAGGCACCTAAAACGCCCCGCCATCAGTAGTATATCACCATTCGGATGCCTTTTAAATTGGTTAAACCGGTTTAAAAAGTGATGAGCGGTAATTAACTACTGATGGGGAGCAAACAGTAATTAACTACCGTTCGAGTTAGGTGCATGTGAGCAATTGCTAGGTGCCTAAAGAGCAATCTCTTGGGATTAATCATGCTTTAGACCTATATATTTATTCGGCCGACCCTATTCCCAAGGGATACTTTTGGTCAATTCCCATATCAAAAAAGATAGATAGAAAAACTTAACCTTAGAGTTTTACCTCTTATTCTTAATGCCATTTCAAACTGTATCATAGCGAGTCATTCTAATGGAAAAAATTAACTGTATTTTCTGCAGCTTCTCAAGCATGGGAGGCCTAG
Protein sequence

>MS.gene58750.t1

MAYSKKHSFLLISTLLMILQLQLSTTNAAIKGGYWNSDSGLAVSDIDPSYFTHLFCAFADLDSNTNQLTISSTNTASFSTFTETVQAKSSSVKTLLSIGGGNSTLAKKFADMASQASSRKSFIDSSIQLARNNNFDGLDLDWEYPSSDADKTNFGLLITEWRAAVAEESSSSGKPTLLLSAAVGGSDQITPLEYYPGQDIANNLDWVNVMTYDLYMSDRYQTQTQPPAPLKNPTGQFSVDEGITKWIGLGVPKSKLALGVPFYGFKWSLSDPNNHEIFDQATQGLGAVKYKDIINVGAQVVYNSTYVTNYCFIGTDWYGYDDTQSISAKVDYAKQNGLFGYFAWNIEQDSNWALSQAASQAWEA