Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64372.t1 | XP_013442068.1 | 89.2 | 194 | 16 | 2 | 1 | 190 | 7 | 199 | 3.50E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64372.t1 | P29602 | 48.1 | 108 | 52 | 4 | 22 | 126 | 5 | 111 | 8.9e-19 | 95.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64372.t1 | A0A072TGI1 | 89.2 | 194 | 16 | 2 | 1 | 190 | 7 | 199 | 2.5e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049634 | MS.gene64372 | 0.839418 | 1.60E-57 | -1.69E-46 |
| MS.gene050083 | MS.gene64372 | 0.81052 | 1.11E-50 | -1.69E-46 |
| MS.gene050095 | MS.gene64372 | 0.813226 | 2.85E-51 | -1.69E-46 |
| MS.gene050096 | MS.gene64372 | 0.822867 | 1.88E-53 | -1.69E-46 |
| MS.gene050380 | MS.gene64372 | 0.803097 | 4.10E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene64372 | 0.84489 | 5.71E-59 | -1.69E-46 |
| MS.gene050809 | MS.gene64372 | 0.837292 | 5.67E-57 | -1.69E-46 |
| MS.gene051127 | MS.gene64372 | 0.802366 | 5.80E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene64372 | 0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene64372 | 0.808013 | 3.82E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene64372 | 0.814338 | 1.62E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene64372 | 0.814058 | 1.87E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene64372 | 0.825912 | 3.62E-54 | -1.69E-46 |
| MS.gene053488 | MS.gene64372 | 0.828935 | 6.82E-55 | -1.69E-46 |
| MS.gene053561 | MS.gene64372 | 0.814753 | 1.31E-51 | -1.69E-46 |
| MS.gene055124 | MS.gene64372 | 0.845618 | 3.63E-59 | -1.69E-46 |
| MS.gene055154 | MS.gene64372 | 0.823418 | 1.40E-53 | -1.69E-46 |
| MS.gene055202 | MS.gene64372 | 0.810486 | 1.13E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene64372 | 0.823972 | 1.04E-53 | -1.69E-46 |
| MS.gene056224 | MS.gene64372 | 0.809543 | 1.80E-50 | -1.69E-46 |
| MS.gene056313 | MS.gene64372 | 0.819235 | 1.29E-52 | -1.69E-46 |
| MS.gene058068 | MS.gene64372 | 0.809171 | 2.16E-50 | -1.69E-46 |
| MS.gene058093 | MS.gene64372 | 0.817575 | 3.07E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene64372 | 0.828264 | 9.90E-55 | -1.69E-46 |
| MS.gene058108 | MS.gene64372 | 0.810137 | 1.34E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene64372 | 0.83222 | 1.07E-55 | -1.69E-46 |
| MS.gene058173 | MS.gene64372 | 0.825249 | 5.19E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene64372 | 0.831129 | 1.99E-55 | -1.69E-46 |
| MS.gene058179 | MS.gene64372 | 0.832378 | 9.78E-56 | -1.69E-46 |
| MS.gene058180 | MS.gene64372 | 0.806811 | 6.86E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene64372 | 0.831714 | 1.43E-55 | -1.69E-46 |
| MS.gene058840 | MS.gene64372 | 0.860651 | 1.78E-63 | -1.69E-46 |
| MS.gene059037 | MS.gene64372 | 0.837124 | 6.26E-57 | -1.69E-46 |
| MS.gene059038 | MS.gene64372 | 0.80543 | 1.34E-49 | -1.69E-46 |
| MS.gene059690 | MS.gene64372 | 0.82899 | 6.61E-55 | -1.69E-46 |
| MS.gene059691 | MS.gene64372 | 0.819725 | 9.99E-53 | -1.69E-46 |
| MS.gene059694 | MS.gene64372 | 0.842215 | 2.96E-58 | -1.69E-46 |
| MS.gene059697 | MS.gene64372 | 0.848528 | 5.79E-60 | -1.69E-46 |
| MS.gene059944 | MS.gene64372 | 0.813859 | 2.07E-51 | -1.69E-46 |
| MS.gene060205 | MS.gene64372 | 0.800347 | 1.50E-48 | -1.69E-46 |
| MS.gene060808 | MS.gene64372 | 0.820633 | 6.19E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64372.t1 | MTR_0334s0010 | 89.175 | 194 | 16 | 2 | 1 | 190 | 7 | 199 | 1.83e-102 | 293 |
| MS.gene64372.t1 | MTR_4g067200 | 50.769 | 195 | 63 | 5 | 1 | 191 | 7 | 172 | 2.94e-54 | 170 |
| MS.gene64372.t1 | MTR_4g066110 | 50.769 | 195 | 63 | 5 | 1 | 191 | 7 | 172 | 2.94e-54 | 170 |
| MS.gene64372.t1 | MTR_6g083240 | 44.516 | 155 | 78 | 4 | 3 | 149 | 8 | 162 | 2.11e-35 | 122 |
| MS.gene64372.t1 | MTR_4g114870 | 49.265 | 136 | 66 | 3 | 3 | 135 | 9 | 144 | 3.75e-34 | 124 |
| MS.gene64372.t1 | MTR_4g114870 | 49.000 | 100 | 50 | 1 | 22 | 120 | 191 | 290 | 4.07e-27 | 105 |
| MS.gene64372.t1 | MTR_4g114830 | 44.755 | 143 | 62 | 5 | 11 | 149 | 17 | 146 | 1.07e-31 | 112 |
| MS.gene64372.t1 | MTR_3g099580 | 42.857 | 105 | 56 | 1 | 17 | 121 | 21 | 121 | 3.18e-24 | 94.4 |
| MS.gene64372.t1 | MTR_2g101300 | 37.762 | 143 | 84 | 4 | 5 | 145 | 9 | 148 | 3.31e-23 | 91.7 |
| MS.gene64372.t1 | MTR_2g090580 | 34.091 | 176 | 109 | 4 | 1 | 172 | 6 | 178 | 3.82e-23 | 91.7 |
| MS.gene64372.t1 | MTR_8g099220 | 39.024 | 123 | 72 | 3 | 1 | 121 | 7 | 128 | 5.63e-23 | 89.4 |
| MS.gene64372.t1 | MTR_3g099570 | 37.692 | 130 | 71 | 2 | 17 | 146 | 21 | 140 | 7.42e-23 | 90.5 |
| MS.gene64372.t1 | MTR_2g088990 | 35.878 | 131 | 79 | 2 | 2 | 131 | 5 | 131 | 5.70e-21 | 86.7 |
| MS.gene64372.t1 | MTR_2g079030 | 36.047 | 172 | 96 | 7 | 4 | 166 | 9 | 175 | 1.58e-20 | 88.6 |
| MS.gene64372.t1 | MTR_8g007020 | 34.884 | 172 | 91 | 5 | 1 | 157 | 1 | 166 | 5.08e-20 | 83.2 |
| MS.gene64372.t1 | MTR_5g006040 | 30.323 | 155 | 103 | 4 | 2 | 154 | 7 | 158 | 5.45e-20 | 83.2 |
| MS.gene64372.t1 | MTR_8g463180 | 35.461 | 141 | 78 | 5 | 4 | 142 | 7 | 136 | 6.99e-20 | 85.1 |
| MS.gene64372.t1 | MTR_6g080660 | 34.711 | 121 | 75 | 1 | 4 | 124 | 10 | 126 | 1.22e-18 | 80.1 |
| MS.gene64372.t1 | MTR_1g014120 | 32.877 | 146 | 86 | 4 | 1 | 142 | 12 | 149 | 1.32e-18 | 79.7 |
| MS.gene64372.t1 | MTR_4g111640 | 29.193 | 161 | 106 | 3 | 3 | 161 | 13 | 167 | 1.45e-18 | 79.3 |
| MS.gene64372.t1 | MTR_1g090420 | 26.633 | 199 | 128 | 4 | 4 | 185 | 10 | 207 | 1.59e-18 | 80.1 |
| MS.gene64372.t1 | MTR_3g099980 | 42.453 | 106 | 55 | 3 | 17 | 120 | 21 | 122 | 1.87e-18 | 80.1 |
| MS.gene64372.t1 | MTR_3g099680 | 42.453 | 106 | 55 | 3 | 17 | 120 | 21 | 122 | 2.94e-18 | 79.3 |
| MS.gene64372.t1 | MTR_7g086230 | 31.013 | 158 | 103 | 3 | 4 | 159 | 7 | 160 | 5.09e-18 | 77.8 |
| MS.gene64372.t1 | MTR_3g105930 | 35.036 | 137 | 83 | 3 | 19 | 149 | 24 | 160 | 6.62e-18 | 77.8 |
| MS.gene64372.t1 | MTR_2g043730 | 36.975 | 119 | 64 | 4 | 1 | 118 | 14 | 122 | 7.26e-18 | 75.9 |
| MS.gene64372.t1 | MTR_4g078410 | 34.694 | 147 | 83 | 5 | 18 | 157 | 21 | 161 | 1.23e-17 | 77.0 |
| MS.gene64372.t1 | MTR_7g086280 | 30.380 | 158 | 104 | 3 | 4 | 159 | 7 | 160 | 2.06e-17 | 76.3 |
| MS.gene64372.t1 | MTR_8g094990 | 33.333 | 123 | 78 | 3 | 1 | 120 | 2 | 123 | 4.95e-17 | 77.0 |
| MS.gene64372.t1 | MTR_8g007035 | 38.318 | 107 | 60 | 2 | 19 | 123 | 21 | 123 | 5.29e-17 | 75.5 |
| MS.gene64372.t1 | MTR_8g095013 | 30.387 | 181 | 120 | 4 | 1 | 178 | 2 | 179 | 5.44e-17 | 77.4 |
| MS.gene64372.t1 | MTR_3g089035 | 39.048 | 105 | 56 | 3 | 15 | 118 | 26 | 123 | 7.88e-17 | 73.6 |
| MS.gene64372.t1 | MTR_8g089110 | 33.898 | 118 | 70 | 3 | 2 | 118 | 8 | 118 | 1.73e-16 | 72.4 |
| MS.gene64372.t1 | MTR_7g086090 | 34.426 | 122 | 74 | 3 | 2 | 121 | 5 | 122 | 3.32e-16 | 74.3 |
| MS.gene64372.t1 | MTR_6g022170 | 35.833 | 120 | 72 | 3 | 11 | 129 | 26 | 141 | 5.30e-16 | 72.4 |
| MS.gene64372.t1 | MTR_8g088830 | 37.736 | 106 | 57 | 4 | 15 | 118 | 21 | 119 | 7.51e-16 | 70.9 |
| MS.gene64372.t1 | MTR_2g090575 | 31.405 | 121 | 80 | 2 | 1 | 120 | 4 | 122 | 8.79e-16 | 74.3 |
| MS.gene64372.t1 | MTR_1g104800 | 27.273 | 154 | 109 | 2 | 6 | 158 | 9 | 160 | 1.24e-15 | 71.6 |
| MS.gene64372.t1 | MTR_1g090190 | 31.159 | 138 | 89 | 2 | 19 | 154 | 20 | 153 | 2.05e-15 | 70.9 |
| MS.gene64372.t1 | MTR_4g077787 | 27.097 | 155 | 104 | 3 | 3 | 149 | 13 | 166 | 3.97e-15 | 70.5 |
| MS.gene64372.t1 | MTR_3g099540 | 35.714 | 126 | 75 | 2 | 1 | 126 | 8 | 127 | 6.05e-15 | 68.6 |
| MS.gene64372.t1 | MTR_6g023760 | 36.893 | 103 | 60 | 3 | 19 | 120 | 23 | 121 | 1.45e-14 | 68.6 |
| MS.gene64372.t1 | MTR_7g086100 | 32.773 | 119 | 74 | 3 | 4 | 120 | 7 | 121 | 2.54e-14 | 68.9 |
| MS.gene64372.t1 | MTR_4g124280 | 29.762 | 168 | 106 | 5 | 3 | 158 | 6 | 173 | 2.87e-14 | 68.9 |
| MS.gene64372.t1 | MTR_2g083250 | 29.897 | 194 | 92 | 6 | 14 | 175 | 18 | 199 | 3.09e-14 | 69.3 |
| MS.gene64372.t1 | MTR_1g072180 | 48.276 | 58 | 30 | 0 | 63 | 120 | 42 | 99 | 6.09e-14 | 65.1 |
| MS.gene64372.t1 | MTR_3g089005 | 31.405 | 121 | 73 | 4 | 1 | 118 | 10 | 123 | 1.85e-13 | 64.7 |
| MS.gene64372.t1 | MTR_4g081100 | 27.869 | 183 | 112 | 4 | 13 | 177 | 23 | 203 | 4.27e-13 | 65.5 |
| MS.gene64372.t1 | MTR_7g086140 | 29.600 | 125 | 81 | 4 | 2 | 123 | 5 | 125 | 5.81e-13 | 63.9 |
| MS.gene64372.t1 | MTR_7g086190 | 29.600 | 125 | 81 | 4 | 2 | 123 | 5 | 125 | 5.81e-13 | 63.9 |
| MS.gene64372.t1 | MTR_7g090170 | 32.353 | 136 | 71 | 4 | 19 | 149 | 24 | 143 | 1.60e-12 | 62.8 |
| MS.gene64372.t1 | MTR_8g086360 | 27.979 | 193 | 120 | 8 | 5 | 188 | 5 | 187 | 2.49e-12 | 63.2 |
| MS.gene64372.t1 | MTR_4g112310 | 28.689 | 122 | 81 | 2 | 40 | 161 | 2 | 117 | 3.30e-12 | 61.2 |
| MS.gene64372.t1 | MTR_7g086220 | 29.730 | 111 | 72 | 3 | 6 | 114 | 8 | 114 | 4.77e-12 | 61.6 |
| MS.gene64372.t1 | MTR_7g086160 | 28.571 | 126 | 82 | 4 | 2 | 123 | 5 | 126 | 7.55e-12 | 61.2 |
| MS.gene64372.t1 | MTR_4g130780 | 28.689 | 122 | 84 | 3 | 3 | 121 | 9 | 130 | 8.50e-12 | 61.2 |
| MS.gene64372.t1 | MTR_8g095020 | 28.571 | 119 | 81 | 3 | 3 | 120 | 6 | 121 | 8.52e-12 | 62.8 |
| MS.gene64372.t1 | MTR_2g025580 | 28.758 | 153 | 101 | 5 | 3 | 152 | 7 | 154 | 1.68e-11 | 60.5 |
| MS.gene64372.t1 | MTR_6g013420 | 32.414 | 145 | 83 | 7 | 3 | 143 | 18 | 151 | 2.14e-11 | 60.1 |
| MS.gene64372.t1 | MTR_2g043790 | 32.692 | 104 | 62 | 3 | 19 | 121 | 4 | 100 | 9.14e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64372.t1 | AT5G20230 | 37.079 | 178 | 95 | 5 | 21 | 186 | 24 | 196 | 3.22e-23 | 92.0 |
| MS.gene64372.t1 | AT2G32300 | 38.971 | 136 | 78 | 3 | 3 | 137 | 7 | 138 | 3.70e-23 | 93.2 |
| MS.gene64372.t1 | AT5G53870 | 35.638 | 188 | 112 | 5 | 1 | 182 | 10 | 194 | 9.38e-23 | 94.0 |
| MS.gene64372.t1 | AT2G32300 | 38.971 | 136 | 78 | 3 | 3 | 137 | 37 | 168 | 1.05e-22 | 92.8 |
| MS.gene64372.t1 | AT5G53870 | 35.638 | 188 | 112 | 5 | 1 | 182 | 10 | 194 | 1.35e-22 | 93.6 |
| MS.gene64372.t1 | AT2G25060 | 36.667 | 150 | 84 | 4 | 6 | 144 | 14 | 163 | 8.65e-22 | 87.8 |
| MS.gene64372.t1 | AT3G20570 | 35.294 | 170 | 102 | 5 | 13 | 176 | 21 | 188 | 2.09e-19 | 82.0 |
| MS.gene64372.t1 | AT4G28365 | 31.683 | 202 | 116 | 9 | 1 | 191 | 9 | 199 | 2.40e-19 | 82.0 |
| MS.gene64372.t1 | AT3G17675 | 29.508 | 122 | 81 | 2 | 1 | 121 | 11 | 128 | 7.58e-19 | 79.0 |
| MS.gene64372.t1 | AT4G27520 | 31.944 | 144 | 89 | 4 | 1 | 137 | 9 | 150 | 2.87e-18 | 81.3 |
| MS.gene64372.t1 | AT4G31840 | 29.050 | 179 | 101 | 4 | 13 | 189 | 18 | 172 | 3.72e-18 | 78.2 |
| MS.gene64372.t1 | AT1G72230 | 38.843 | 121 | 69 | 2 | 40 | 158 | 37 | 154 | 1.13e-17 | 77.0 |
| MS.gene64372.t1 | AT3G60270 | 36.000 | 100 | 60 | 1 | 24 | 123 | 28 | 123 | 1.59e-17 | 76.6 |
| MS.gene64372.t1 | AT5G07475 | 30.882 | 136 | 87 | 2 | 7 | 142 | 15 | 143 | 4.14e-17 | 75.9 |
| MS.gene64372.t1 | AT2G26720 | 33.582 | 134 | 78 | 4 | 3 | 132 | 14 | 140 | 4.42e-17 | 76.3 |
| MS.gene64372.t1 | AT3G27200 | 28.926 | 121 | 80 | 3 | 2 | 120 | 5 | 121 | 2.09e-15 | 70.9 |
| MS.gene64372.t1 | AT2G44790 | 39.080 | 87 | 51 | 1 | 40 | 124 | 42 | 128 | 3.90e-15 | 70.9 |
| MS.gene64372.t1 | AT5G26330 | 32.520 | 123 | 78 | 3 | 1 | 120 | 1 | 121 | 6.31e-15 | 70.1 |
| MS.gene64372.t1 | AT2G31050 | 34.815 | 135 | 78 | 4 | 2 | 132 | 12 | 140 | 7.86e-15 | 70.1 |
| MS.gene64372.t1 | AT1G79800 | 33.333 | 138 | 83 | 3 | 3 | 132 | 15 | 151 | 1.81e-14 | 68.9 |
| MS.gene64372.t1 | AT3G18590 | 32.787 | 122 | 73 | 4 | 3 | 120 | 10 | 126 | 7.65e-14 | 67.0 |
| MS.gene64372.t1 | AT4G32490 | 32.836 | 134 | 82 | 5 | 1 | 132 | 11 | 138 | 8.03e-14 | 67.8 |
| MS.gene64372.t1 | AT1G22480 | 41.772 | 79 | 46 | 0 | 47 | 125 | 40 | 118 | 8.63e-14 | 66.6 |
| MS.gene64372.t1 | AT3G60280 | 34.286 | 105 | 65 | 1 | 19 | 123 | 21 | 121 | 4.04e-13 | 65.9 |
| MS.gene64372.t1 | AT5G57920 | 27.273 | 121 | 86 | 2 | 2 | 120 | 3 | 123 | 5.06e-13 | 64.7 |
| MS.gene64372.t1 | AT5G14345 | 30.189 | 106 | 72 | 2 | 29 | 133 | 23 | 127 | 8.96e-13 | 63.2 |
| MS.gene64372.t1 | AT4G30590 | 32.039 | 103 | 68 | 2 | 24 | 124 | 30 | 132 | 1.07e-12 | 63.9 |
| MS.gene64372.t1 | AT5G57920 | 26.812 | 138 | 94 | 3 | 17 | 147 | 50 | 187 | 1.25e-12 | 64.3 |
| MS.gene64372.t1 | AT1G48940 | 29.412 | 119 | 78 | 3 | 4 | 120 | 11 | 125 | 4.04e-12 | 62.4 |
| MS.gene64372.t1 | AT5G57920 | 31.313 | 99 | 66 | 2 | 24 | 120 | 24 | 122 | 5.64e-12 | 62.0 |
| MS.gene64372.t1 | AT2G27035 | 32.903 | 155 | 89 | 7 | 2 | 153 | 8 | 150 | 8.39e-12 | 61.2 |
| MS.gene64372.t1 | AT2G02850 | 30.833 | 120 | 73 | 4 | 2 | 118 | 14 | 126 | 1.47e-11 | 59.7 |
| MS.gene64372.t1 | AT5G15350 | 34.266 | 143 | 79 | 7 | 6 | 144 | 12 | 143 | 5.46e-11 | 58.9 |
| MS.gene64372.t1 | AT2G23990 | 30.400 | 125 | 84 | 3 | 18 | 139 | 23 | 147 | 6.44e-11 | 59.3 |
Find 55 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTTTAACGCTTAGCTTT+TGG | 0.290349 | 2.4:+36167830 | None:intergenic |
| AATTGCAACATCAATCAAAA+TGG | 0.366532 | 2.4:-36167946 | MS.gene64372:CDS |
| CTGTTAAGAGTAACTCTTGC+TGG | 0.373715 | 2.4:+36167904 | None:intergenic |
| ATGATAACCGCAATGAGAAC+AGG | 0.411992 | 2.4:+36167631 | None:intergenic |
| CTGGTCCTGTTGTAATGACT+TGG | 0.420784 | 2.4:+36167923 | None:intergenic |
| GGAGTAGATGTAGGAGGAGT+AGG | 0.442205 | 2.4:+36167781 | None:intergenic |
| AGAGTTACTCTTAACAGAAC+TGG | 0.466599 | 2.4:-36167898 | MS.gene64372:CDS |
| ACTGGAACAATGACCTTGAA+TGG | 0.487188 | 2.4:+36167855 | None:intergenic |
| GTAGGAGGAGTAGGAGAAGG+AGG | 0.487629 | 2.4:+36167790 | None:intergenic |
| CCTCCGTTGGTAGGTGGAGT+AGG | 0.491660 | 2.4:+36167736 | None:intergenic |
| TTGTTTGAATGTATTATTGG+CGG | 0.499396 | 2.4:+36169343 | None:intergenic |
| CATACTGTTGGAGGCACCAC+TGG | 0.499434 | 2.4:-36169410 | MS.gene64372:CDS |
| AATGGCCAAGTCATTACAAC+AGG | 0.504661 | 2.4:-36167928 | MS.gene64372:CDS |
| ACGAGAGTGGTAGCTGAGCC+TGG | 0.506000 | 2.4:+36167664 | None:intergenic |
| TGGAGGAGTTGCATCGGGCC+CGG | 0.506285 | 2.4:+36167690 | None:intergenic |
| GGTGATGGAGGAGTTGCATC+GGG | 0.508604 | 2.4:+36167685 | None:intergenic |
| TGGTGATGGAGGAGTTGCAT+CGG | 0.517659 | 2.4:+36167684 | None:intergenic |
| TAAAACGAAGCGCCGCCACT+TGG | 0.519955 | 2.4:+36169377 | None:intergenic |
| GGAGTTGCATCGGGCCCGGT+GGG | 0.523382 | 2.4:+36167694 | None:intergenic |
| GTAGGAGGAGGAGTAGATGT+AGG | 0.523768 | 2.4:+36167772 | None:intergenic |
| TTCAGCAACAGTGTGGCCTC+CGG | 0.526784 | 2.4:+36167990 | None:intergenic |
| TCTACTCCTCCTCCTACATC+AGG | 0.528805 | 2.4:-36167766 | MS.gene64372:CDS |
| AGGAGAAGGAGGAGAAGTCG+AGG | 0.539764 | 2.4:+36167801 | None:intergenic |
| CGGCGAAGGTGTTCCTCCGT+TGG | 0.540474 | 2.4:+36167723 | None:intergenic |
| CACTGTTGCTGAAGTAAGCA+AGG | 0.543847 | 2.4:-36167980 | MS.gene64372:CDS |
| GAAGGTGTTCCTCCGTTGGT+AGG | 0.546863 | 2.4:+36167727 | None:intergenic |
| AGGAGTTGCATCGGGCCCGG+TGG | 0.547277 | 2.4:+36167693 | None:intergenic |
| TCGGGCCCGGTGGGAGATGA+CGG | 0.548024 | 2.4:+36167703 | None:intergenic |
| GAGGCAGCAGATCATACTGT+TGG | 0.551162 | 2.4:-36169422 | MS.gene64372:CDS |
| GATGTAGGAGGAGTAGGAGA+AGG | 0.553864 | 2.4:+36167787 | None:intergenic |
| GTAGGAGTAGTTCCTGATGT+AGG | 0.559933 | 2.4:+36167754 | None:intergenic |
| GTGGTAGCTGAGCCTGGTGA+TGG | 0.561174 | 2.4:+36167670 | None:intergenic |
| GATGCAACTCCTCCATCACC+AGG | 0.561384 | 2.4:-36167682 | MS.gene64372:CDS |
| TTCAAACAAAACGATGTCCT+AGG | 0.564087 | 2.4:-36169329 | MS.gene64372:intron |
| CCGGTGGGAGATGACGGCGA+AGG | 0.570502 | 2.4:+36167709 | None:intergenic |
| GGTGTTCCTCCGTTGGTAGG+TGG | 0.575863 | 2.4:+36167730 | None:intergenic |
| CCTTCGCCGTCATCTCCCAC+CGG | 0.576478 | 2.4:-36167709 | MS.gene64372:CDS |
| GGAGGAGGAGTAGATGTAGG+AGG | 0.578805 | 2.4:+36167775 | None:intergenic |
| GTAGTTCCTGATGTAGGAGG+AGG | 0.581223 | 2.4:+36167760 | None:intergenic |
| GCAGCAGATCATACTGTTGG+AGG | 0.591168 | 2.4:-36169419 | MS.gene64372:CDS |
| TACGTTCCCTGTTCTCATTG+CGG | 0.597691 | 2.4:-36167638 | MS.gene64372:CDS |
| ATTCAAGGTCATTGTTCCAG+TGG | 0.598707 | 2.4:-36167853 | MS.gene64372:CDS |
| GGTTGGAGTGTTCCAAGTGG+CGG | 0.608033 | 2.4:-36169389 | MS.gene64372:CDS |
| CTGTTGGAGGCACCACTGGT+TGG | 0.613559 | 2.4:-36169406 | MS.gene64372:CDS |
| TGCTTACTTCAGCAACAGTG+TGG | 0.617452 | 2.4:+36167983 | None:intergenic |
| GGAGTAGTTCCTGATGTAGG+AGG | 0.617793 | 2.4:+36167757 | None:intergenic |
| CTTCGCCGTCATCTCCCACC+GGG | 0.621161 | 2.4:-36167708 | MS.gene64372:CDS |
| GTAGCTGAGCCTGGTGATGG+AGG | 0.629395 | 2.4:+36167673 | None:intergenic |
| TGATAACCGCAATGAGAACA+GGG | 0.653429 | 2.4:+36167632 | None:intergenic |
| ACTTGGAACACTCCAACCAG+TGG | 0.663713 | 2.4:+36169394 | None:intergenic |
| ACTCCTACTCCACCTACCAA+CGG | 0.675324 | 2.4:-36167739 | MS.gene64372:CDS |
| AGGGAACGTAGCAACGAGAG+TGG | 0.679886 | 2.4:+36167651 | None:intergenic |
| ACTGGTTGGAGTGTTCCAAG+TGG | 0.717431 | 2.4:-36169392 | MS.gene64372:CDS |
| CCTACTCCACCTACCAACGG+AGG | 0.737055 | 2.4:-36167736 | MS.gene64372:CDS |
| AGCAATATTAGAAAGCACAG+AGG | 0.775343 | 2.4:-36169441 | MS.gene64372:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATATTTATTATTCTAAA+TGG | + | chr2.4:36168370-36168389 | None:intergenic | 10.0% |
| !! | TCTGTTAATAATTAAATTAA+TGG | - | chr2.4:36168097-36168116 | MS.gene64372:intron | 10.0% |
| !! | TTTGTTTACTATAAAATATA+TGG | + | chr2.4:36168020-36168039 | None:intergenic | 10.0% |
| !!! | ATTTTTTTTAAGTAGTTTAA+TGG | + | chr2.4:36168545-36168564 | None:intergenic | 10.0% |
| !! | AAAATGAGACTATTAAAATA+AGG | + | chr2.4:36169043-36169062 | None:intergenic | 15.0% |
| !!! | AAATCTTATCTTTTGTATTT+GGG | - | chr2.4:36167883-36167902 | MS.gene64372:CDS | 15.0% |
| !!! | TAAATCTTATCTTTTGTATT+TGG | - | chr2.4:36167882-36167901 | MS.gene64372:CDS | 15.0% |
| !!! | TTTGTTATTCTATAATAAGT+TGG | + | chr2.4:36168307-36168326 | None:intergenic | 15.0% |
| !!! | TTTTTTTTTTTTGTTTGTTG+AGG | + | chr2.4:36168773-36168792 | None:intergenic | 15.0% |
| !! | ACTGTTATTCTATAATAAGT+TGG | + | chr2.4:36168277-36168296 | None:intergenic | 20.0% |
| !! | TGAATAAACACTTAATAAAG+AGG | + | chr2.4:36168600-36168619 | None:intergenic | 20.0% |
| !!! | GTTTTGTTTGAATGTATTAT+TGG | + | chr2.4:36167751-36167770 | None:intergenic | 20.0% |
| !!! | TATTTTATATGTACCATTCA+AGG | - | chr2.4:36169220-36169239 | MS.gene64372:intron | 20.0% |
| !!! | TCTTTTACATCTTGAAAATA+GGG | + | chr2.4:36168994-36169013 | None:intergenic | 20.0% |
| !!! | TTTTTTCTTCTTCAAGTTAT+CGG | - | chr2.4:36168726-36168745 | MS.gene64372:intron | 20.0% |
| ! | AATAGCAATGAGATATACAT+AGG | - | chr2.4:36168681-36168700 | MS.gene64372:intron | 25.0% |
| ! | AATTGAAAACTGAAATTGGA+CGG | + | chr2.4:36169075-36169094 | None:intergenic | 25.0% |
| ! | AATTGCAACATCAATCAAAA+TGG | - | chr2.4:36169142-36169161 | MS.gene64372:intron | 25.0% |
| ! | ATAGCAATGAGATATACATA+GGG | - | chr2.4:36168682-36168701 | MS.gene64372:intron | 25.0% |
| ! | ATTATAGAATAACAAACCCT+AGG | - | chr2.4:36168311-36168330 | MS.gene64372:intron | 25.0% |
| ! | CTATTGTTAGAATAAGCAAT+TGG | + | chr2.4:36168830-36168849 | None:intergenic | 25.0% |
| ! | GCAAAATTGAAAACTGAAAT+TGG | + | chr2.4:36169079-36169098 | None:intergenic | 25.0% |
| ! | TCAACTATCAAGATCTAATA+TGG | + | chr2.4:36168076-36168095 | None:intergenic | 25.0% |
| ! | TTATAAGTATGAAGAAGTGA+AGG | + | chr2.4:36168509-36168528 | None:intergenic | 25.0% |
| ! | TTGTTTGAATGTATTATTGG+CGG | + | chr2.4:36167748-36167767 | None:intergenic | 25.0% |
| !! | ATTTAGTTCCAAGTTTGTTT+AGG | + | chr2.4:36167867-36167886 | None:intergenic | 25.0% |
| !! | ATTTCAGTTTTCAATTTTGC+CGG | - | chr2.4:36169079-36169098 | MS.gene64372:intron | 25.0% |
| !! | CTCTTTTACATCTTGAAAAT+AGG | + | chr2.4:36168995-36169014 | None:intergenic | 25.0% |
| ATCTGTTCAAAAAATCTCTG+TGG | + | chr2.4:36167956-36167975 | None:intergenic | 30.0% | |
| CAGTATCGTGAACTTAAAAT+TGG | + | chr2.4:36168141-36168160 | None:intergenic | 30.0% | |
| GATTCTCTCCATTTAGAAAA+TGG | + | chr2.4:36168454-36168473 | None:intergenic | 30.0% | |
| TACATTCTCCTAAACAAACT+TGG | - | chr2.4:36167856-36167875 | MS.gene64372:CDS | 30.0% | |
| TTTACATCTTGAAAATAGGG+AGG | + | chr2.4:36168991-36169010 | None:intergenic | 30.0% | |
| ! | TTTTGAACAGATGGTTGTAA+CGG | - | chr2.4:36167964-36167983 | MS.gene64372:CDS | 30.0% |
| !! | AAACTTTAACGCTTAGCTTT+TGG | + | chr2.4:36169261-36169280 | None:intergenic | 30.0% |
| !! | TCTGAAATGTTGGTTTATAG+TGG | - | chr2.4:36168409-36168428 | MS.gene64372:intron | 30.0% |
| !!! | ACAGAGATTTTTTGAACAGA+TGG | - | chr2.4:36167955-36167974 | MS.gene64372:CDS | 30.0% |
| AGAATCATAACTCAATGGCA+GGG | - | chr2.4:36168468-36168487 | MS.gene64372:intron | 35.0% | |
| AGAGTTACTCTTAACAGAAC+TGG | - | chr2.4:36169190-36169209 | MS.gene64372:intron | 35.0% | |
| GTATGAAGAAGTGAAGGATA+TGG | + | chr2.4:36168503-36168522 | None:intergenic | 35.0% | |
| TAGAATAACAAACCCTAGGT+TGG | - | chr2.4:36168315-36168334 | MS.gene64372:intron | 35.0% | |
| TCTCCATTTAGAAAATGGAG+AGG | + | chr2.4:36168449-36168468 | None:intergenic | 35.0% | |
| TGGAGAGAATCATAACTCAA+TGG | - | chr2.4:36168463-36168482 | MS.gene64372:intron | 35.0% | |
| TGTTGAATTAGAGCTGAGTT+TGG | + | chr2.4:36168647-36168666 | None:intergenic | 35.0% | |
| TTCAAACAAAACGATGTCCT+AGG | - | chr2.4:36167759-36167778 | MS.gene64372:CDS | 35.0% | |
| ! | AGCAATATTAGAAAGCACAG+AGG | - | chr2.4:36167647-36167666 | MS.gene64372:CDS | 35.0% |
| ! | AGTCCTCTCCATTTTCTAAA+TGG | - | chr2.4:36168443-36168462 | MS.gene64372:intron | 35.0% |
| !! | AAATGTTGGTTTATAGTGGC+AGG | - | chr2.4:36168413-36168432 | MS.gene64372:intron | 35.0% |
| !! | AATGTTGGTTTATAGTGGCA+GGG | - | chr2.4:36168414-36168433 | MS.gene64372:intron | 35.0% |
| !! | GGTAACAAACTCTGAAATGT+TGG | - | chr2.4:36168399-36168418 | MS.gene64372:intron | 35.0% |
| AATGGCCAAGTCATTACAAC+AGG | - | chr2.4:36169160-36169179 | MS.gene64372:intron | 40.0% | |
| ACTGGAACAATGACCTTGAA+TGG | + | chr2.4:36169236-36169255 | None:intergenic | 40.0% | |
| ATGATAACCGCAATGAGAAC+AGG | + | chr2.4:36169460-36169479 | None:intergenic | 40.0% | |
| ATTCAAGGTCATTGTTCCAG+TGG | - | chr2.4:36169235-36169254 | MS.gene64372:intron | 40.0% | |
| CTGTTAAGAGTAACTCTTGC+TGG | + | chr2.4:36169187-36169206 | None:intergenic | 40.0% | |
| GAATCATAACTCAATGGCAG+GGG | - | chr2.4:36168469-36168488 | MS.gene64372:intron | 40.0% | |
| GAGAATCATAACTCAATGGC+AGG | - | chr2.4:36168467-36168486 | MS.gene64372:intron | 40.0% | |
| GCATATACACAAACTCACCT+AGG | + | chr2.4:36167779-36167798 | None:intergenic | 40.0% | |
| TGATAACCGCAATGAGAACA+GGG | + | chr2.4:36169459-36169478 | None:intergenic | 40.0% | |
| ! | CGCTTCGTTTTACTCAGATT+GGG | - | chr2.4:36167722-36167741 | MS.gene64372:CDS | 40.0% |
| ! | TCAGTTTTCAATTTTGCCGG+AGG | - | chr2.4:36169082-36169101 | MS.gene64372:intron | 40.0% |
| CACTGTTGCTGAAGTAAGCA+AGG | - | chr2.4:36169108-36169127 | MS.gene64372:intron | 45.0% | |
| CTGGTCCTGTTGTAATGACT+TGG | + | chr2.4:36169168-36169187 | None:intergenic | 45.0% | |
| GTAGGAGTAGTTCCTGATGT+AGG | + | chr2.4:36169337-36169356 | None:intergenic | 45.0% | |
| TACGTTCCCTGTTCTCATTG+CGG | - | chr2.4:36169450-36169469 | MS.gene64372:CDS | 45.0% | |
| TGCTTACTTCAGCAACAGTG+TGG | + | chr2.4:36169108-36169127 | None:intergenic | 45.0% | |
| ! | GCGCTTCGTTTTACTCAGAT+TGG | - | chr2.4:36167721-36167740 | MS.gene64372:CDS | 45.0% |
| !! | AGCTCAGTTGGTAACTCAGT+TGG | - | chr2.4:36168339-36168358 | MS.gene64372:intron | 45.0% |
| !! | AAAAAGATTAATTAATTTAT+TGG | + | chr2.4:36168709-36168728 | None:intergenic | 5.0% |
| !! | AATAAATATTGAATATTTAA+GGG | - | chr2.4:36168378-36168397 | MS.gene64372:intron | 5.0% |
| !! | TAATAAATATTGAATATTTA+AGG | - | chr2.4:36168377-36168396 | MS.gene64372:intron | 5.0% |
| ACCAACTGAGCTACCAACCT+AGG | + | chr2.4:36168331-36168350 | None:intergenic | 50.0% | |
| ACTCCTACTCCACCTACCAA+CGG | - | chr2.4:36169349-36169368 | MS.gene64372:CDS | 50.0% | |
| ACTTGGAACACTCCAACCAG+TGG | + | chr2.4:36167697-36167716 | None:intergenic | 50.0% | |
| CCAACTGAGCTACCAACCTA+GGG | + | chr2.4:36168330-36168349 | None:intergenic | 50.0% | |
| GAGGCAGCAGATCATACTGT+TGG | - | chr2.4:36167666-36167685 | MS.gene64372:CDS | 50.0% | |
| GATGTAGGAGGAGTAGGAGA+AGG | + | chr2.4:36169304-36169323 | None:intergenic | 50.0% | |
| GCAGCAGATCATACTGTTGG+AGG | - | chr2.4:36167669-36167688 | MS.gene64372:CDS | 50.0% | |
| GGAGTAGATGTAGGAGGAGT+AGG | + | chr2.4:36169310-36169329 | None:intergenic | 50.0% | |
| GGAGTAGTTCCTGATGTAGG+AGG | + | chr2.4:36169334-36169353 | None:intergenic | 50.0% | |
| GTAGGAGGAGGAGTAGATGT+AGG | + | chr2.4:36169319-36169338 | None:intergenic | 50.0% | |
| GTAGTTCCTGATGTAGGAGG+AGG | + | chr2.4:36169331-36169350 | None:intergenic | 50.0% | |
| TCTACTCCTCCTCCTACATC+AGG | - | chr2.4:36169322-36169341 | MS.gene64372:intron | 50.0% | |
| ! | ACTGGTTGGAGTGTTCCAAG+TGG | - | chr2.4:36167696-36167715 | MS.gene64372:CDS | 50.0% |
| !! | AACGCTTAGCTTTTGGCCAC+TGG | + | chr2.4:36169254-36169273 | None:intergenic | 50.0% |
| !! | TGGTGATGGAGGAGTTGCAT+CGG | + | chr2.4:36169407-36169426 | None:intergenic | 50.0% |
| AGGAGAAGGAGGAGAAGTCG+AGG | + | chr2.4:36169290-36169309 | None:intergenic | 55.0% | |
| AGGGAACGTAGCAACGAGAG+TGG | + | chr2.4:36169440-36169459 | None:intergenic | 55.0% | |
| GATGCAACTCCTCCATCACC+AGG | - | chr2.4:36169406-36169425 | MS.gene64372:CDS | 55.0% | |
| GGAGGAGGAGTAGATGTAGG+AGG | + | chr2.4:36169316-36169335 | None:intergenic | 55.0% | |
| GTAGGAGGAGTAGGAGAAGG+AGG | + | chr2.4:36169301-36169320 | None:intergenic | 55.0% | |
| TTCAGCAACAGTGTGGCCTC+CGG | + | chr2.4:36169101-36169120 | None:intergenic | 55.0% | |
| ! | GGTTGGAGTGTTCCAAGTGG+CGG | - | chr2.4:36167699-36167718 | MS.gene64372:CDS | 55.0% |
| !! | CATACTGTTGGAGGCACCAC+TGG | - | chr2.4:36167678-36167697 | MS.gene64372:CDS | 55.0% |
| !! | CCCTAGGTTGGTAGCTCAGT+TGG | - | chr2.4:36168327-36168346 | MS.gene64372:intron | 55.0% |
| !! | GAAGGTGTTCCTCCGTTGGT+AGG | + | chr2.4:36169364-36169383 | None:intergenic | 55.0% |
| !! | GGTGATGGAGGAGTTGCATC+GGG | + | chr2.4:36169406-36169425 | None:intergenic | 55.0% |
| !! | TAAAACGAAGCGCCGCCACT+TGG | + | chr2.4:36167714-36167733 | None:intergenic | 55.0% |
| ACGAGAGTGGTAGCTGAGCC+TGG | + | chr2.4:36169427-36169446 | None:intergenic | 60.0% | |
| CCTACTCCACCTACCAACGG+AGG | - | chr2.4:36169352-36169371 | MS.gene64372:CDS | 60.0% | |
| GTGGTAGCTGAGCCTGGTGA+TGG | + | chr2.4:36169421-36169440 | None:intergenic | 60.0% | |
| ! | CCTCCGTTGGTAGGTGGAGT+AGG | + | chr2.4:36169355-36169374 | None:intergenic | 60.0% |
| ! | GGTGTTCCTCCGTTGGTAGG+TGG | + | chr2.4:36169361-36169380 | None:intergenic | 60.0% |
| !! | CTGTTGGAGGCACCACTGGT+TGG | - | chr2.4:36167682-36167701 | MS.gene64372:CDS | 60.0% |
| !! | GTAGCTGAGCCTGGTGATGG+AGG | + | chr2.4:36169418-36169437 | None:intergenic | 60.0% |
| CCTTCGCCGTCATCTCCCAC+CGG | - | chr2.4:36169379-36169398 | MS.gene64372:CDS | 65.0% | |
| CTTCGCCGTCATCTCCCACC+GGG | - | chr2.4:36169380-36169399 | MS.gene64372:CDS | 65.0% | |
| TGGAGGAGTTGCATCGGGCC+CGG | + | chr2.4:36169401-36169420 | None:intergenic | 65.0% | |
| !! | CGGCGAAGGTGTTCCTCCGT+TGG | + | chr2.4:36169368-36169387 | None:intergenic | 65.0% |
| AGGAGTTGCATCGGGCCCGG+TGG | + | chr2.4:36169398-36169417 | None:intergenic | 70.0% | |
| GGAGTTGCATCGGGCCCGGT+GGG | + | chr2.4:36169397-36169416 | None:intergenic | 70.0% | |
| TCGGGCCCGGTGGGAGATGA+CGG | + | chr2.4:36169388-36169407 | None:intergenic | 70.0% | |
| ! | CCGGTGGGAGATGACGGCGA+AGG | + | chr2.4:36169382-36169401 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 36167615 | 36169495 | 36167615 | ID=MS.gene64372 |
| chr2.4 | mRNA | 36167615 | 36169495 | 36167615 | ID=MS.gene64372.t1;Parent=MS.gene64372 |
| chr2.4 | exon | 36169330 | 36169495 | 36169330 | ID=MS.gene64372.t1.exon1;Parent=MS.gene64372.t1 |
| chr2.4 | CDS | 36169330 | 36169495 | 36169330 | ID=cds.MS.gene64372.t1;Parent=MS.gene64372.t1 |
| chr2.4 | exon | 36167615 | 36168024 | 36167615 | ID=MS.gene64372.t1.exon2;Parent=MS.gene64372.t1 |
| chr2.4 | CDS | 36167615 | 36168024 | 36167615 | ID=cds.MS.gene64372.t1;Parent=MS.gene64372.t1 |
| Gene Sequence |
| Protein sequence |
