Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67002.t1 | XP_003589130.1 | 96.9 | 131 | 4 | 0 | 1 | 131 | 49 | 179 | 5.00E-73 | 283.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67002.t1 | K4PW38 | 47.4 | 114 | 50 | 3 | 19 | 128 | 74 | 181 | 5.0e-21 | 102.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67002.t1 | Q2HTQ7 | 96.9 | 131 | 4 | 0 | 1 | 131 | 49 | 179 | 3.6e-73 | 283.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene67002 | 0.816289 | 5.97E-52 | -1.69E-46 |
| MS.gene049758 | MS.gene67002 | 0.815727 | 7.97E-52 | -1.69E-46 |
| MS.gene050516 | MS.gene67002 | 0.821376 | 4.17E-53 | -1.69E-46 |
| MS.gene050584 | MS.gene67002 | 0.801476 | 8.84E-49 | -1.69E-46 |
| MS.gene050585 | MS.gene67002 | 0.812332 | 4.47E-51 | -1.69E-46 |
| MS.gene050586 | MS.gene67002 | 0.840927 | 6.47E-58 | -1.69E-46 |
| MS.gene050762 | MS.gene67002 | 0.836571 | 8.66E-57 | -1.69E-46 |
| MS.gene050763 | MS.gene67002 | 0.838142 | 3.43E-57 | -1.69E-46 |
| MS.gene050807 | MS.gene67002 | 0.808258 | 3.38E-50 | -1.69E-46 |
| MS.gene051626 | MS.gene67002 | 0.80295 | 4.40E-49 | -1.69E-46 |
| MS.gene051789 | MS.gene67002 | 0.849467 | 3.17E-60 | -1.69E-46 |
| MS.gene052255 | MS.gene67002 | 0.801396 | 9.19E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene67002 | 0.81599 | 6.96E-52 | -1.69E-46 |
| MS.gene053074 | MS.gene67002 | 0.847302 | 1.26E-59 | -1.69E-46 |
| MS.gene053204 | MS.gene67002 | 0.831832 | 1.33E-55 | -1.69E-46 |
| MS.gene053354 | MS.gene67002 | 0.816372 | 5.72E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene67002 | 0.81866 | 1.75E-52 | -1.69E-46 |
| MS.gene054010 | MS.gene67002 | 0.815971 | 7.03E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene67002 | 0.81411 | 1.82E-51 | -1.69E-46 |
| MS.gene054012 | MS.gene67002 | 0.822924 | 1.83E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene67002 | 0.819453 | 1.15E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene67002 | 0.820499 | 6.64E-53 | -1.69E-46 |
| MS.gene054185 | MS.gene67002 | 0.815208 | 1.04E-51 | -1.69E-46 |
| MS.gene054214 | MS.gene67002 | 0.819138 | 1.36E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene67002 | 0.830916 | 2.24E-55 | -1.69E-46 |
| MS.gene054473 | MS.gene67002 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene05456 | MS.gene67002 | 0.808229 | 3.43E-50 | -1.69E-46 |
| MS.gene054596 | MS.gene67002 | 0.817477 | 3.23E-52 | -1.69E-46 |
| MS.gene055303 | MS.gene67002 | 0.803661 | 3.13E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene67002 | 0.839929 | 1.18E-57 | -1.69E-46 |
| MS.gene056069 | MS.gene67002 | -0.810376 | 1.19E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene67002 | 0.828229 | 1.01E-54 | -1.69E-46 |
| MS.gene058168 | MS.gene67002 | 0.83359 | 4.89E-56 | -1.69E-46 |
| MS.gene059006 | MS.gene67002 | 0.839881 | 1.22E-57 | -1.69E-46 |
| MS.gene059124 | MS.gene67002 | 0.82758 | 1.45E-54 | -1.69E-46 |
| MS.gene059421 | MS.gene67002 | 0.807413 | 5.12E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene67002 | 0.805093 | 1.58E-49 | -1.69E-46 |
| MS.gene060320 | MS.gene67002 | 0.809692 | 1.67E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene67002 | 0.800772 | 1.23E-48 | -1.69E-46 |
| MS.gene060404 | MS.gene67002 | 0.811761 | 5.96E-51 | -1.69E-46 |
| MS.gene060476 | MS.gene67002 | 0.826552 | 2.55E-54 | -1.69E-46 |
| MS.gene060480 | MS.gene67002 | 0.819942 | 8.91E-53 | -1.69E-46 |
| MS.gene060559 | MS.gene67002 | 0.806362 | 8.53E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTTAAGGTGGGAATT+TGG | 0.274976 | 1.3:+4452560 | None:intergenic |
| TCAGTTAAATTCAGGCATTC+AGG | 0.281549 | 1.3:+4453477 | MS.gene67002:CDS |
| CCAAGGGAAATAAAAGAAAT+TGG | 0.316828 | 1.3:+4452603 | MS.gene67002:CDS |
| GTTTATAGTGACACTCAAAA+TGG | 0.326390 | 1.3:+4453075 | MS.gene67002:CDS |
| TCTCATGAGGTTTATAGTTA+TGG | 0.339420 | 1.3:+4452875 | MS.gene67002:CDS |
| GGATGCTTGAATGAAAGATT+TGG | 0.362727 | 1.3:+4452830 | MS.gene67002:CDS |
| TGTTTGTTAGGATTATTGTA+TGG | 0.365430 | 1.3:+4452762 | MS.gene67002:intron |
| TGGGAGTTTCAGTTAAATTC+AGG | 0.382777 | 1.3:+4453469 | MS.gene67002:CDS |
| GGGAAAGTTGCAGCAGATAA+TGG | 0.383947 | 1.3:+4453042 | MS.gene67002:CDS |
| ATAAATATTATGCAGATTAG+TGG | 0.407993 | 1.3:+4453020 | MS.gene67002:intron |
| AGGATTATTGTATGGGAAGA+TGG | 0.415442 | 1.3:+4452770 | MS.gene67002:intron |
| CAGCAGATAATGGTCACAAA+TGG | 0.418083 | 1.3:+4453052 | MS.gene67002:CDS |
| TAAATATTATGCAGATTAGT+GGG | 0.421333 | 1.3:+4453021 | MS.gene67002:intron |
| AACTGAAACTCCCATGCCCT+TGG | 0.450146 | 1.3:-4453460 | None:intergenic |
| GCTGTCATTGCTGTCAAAGA+AGG | 0.450482 | 1.3:+4453650 | MS.gene67002:CDS |
| AGCAGATAATGGTCACAAAT+GGG | 0.486308 | 1.3:+4453053 | MS.gene67002:CDS |
| GAATGTGAGCAAAGGAAGAA+TGG | 0.490985 | 1.3:+4452809 | MS.gene67002:CDS |
| TTTGACAGCAACCAAGGGCA+TGG | 0.491667 | 1.3:+4453449 | MS.gene67002:intron |
| TTTATAGTGACACTCAAAAT+GGG | 0.504799 | 1.3:+4453076 | MS.gene67002:CDS |
| GTTTGTTAGGATTATTGTAT+GGG | 0.510760 | 1.3:+4452763 | MS.gene67002:intron |
| GACTTCTCTTGATCGTTCCA+AGG | 0.518829 | 1.3:+4452586 | MS.gene67002:CDS |
| TTGACAGCAACCAAGGGCAT+GGG | 0.522018 | 1.3:+4453450 | MS.gene67002:intron |
| GAGGTTTATAGTTATGGAGA+AGG | 0.523360 | 1.3:+4452881 | MS.gene67002:CDS |
| TGTTTAAGGTGGGAATTTGG+AGG | 0.535611 | 1.3:+4452563 | None:intergenic |
| GTTTAAGGTGGGAATTTGGA+GGG | 0.541664 | 1.3:+4452564 | None:intergenic |
| AGGTTTATAGTTATGGAGAA+GGG | 0.550052 | 1.3:+4452882 | MS.gene67002:CDS |
| AAAGAAGGCTTAGTACAACT+TGG | 0.558403 | 1.3:+4453665 | MS.gene67002:CDS |
| TGATCAATTGAAGAATTCCA+TGG | 0.573918 | 1.3:-4453120 | None:intergenic |
| ATTCTTCAAAATGTCTCATG+AGG | 0.574625 | 1.3:+4452862 | MS.gene67002:CDS |
| ATGCTAACTATGTTGGTCCA+TGG | 0.581522 | 1.3:+4453103 | MS.gene67002:CDS |
| ACTTCTCTTGATCGTTCCAA+GGG | 0.587449 | 1.3:+4452587 | MS.gene67002:CDS |
| ACAACTTGGTTCATTTAACA+AGG | 0.613113 | 1.3:+4453679 | MS.gene67002:CDS |
| AAATATTATGCAGATTAGTG+GGG | 0.628220 | 1.3:+4453022 | MS.gene67002:intron |
| GGGTGTGATGCTAACTATGT+TGG | 0.633314 | 1.3:+4453096 | MS.gene67002:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATCTTATTTTCTTAAACA+TGG | - | chr1.3:4452947-4452966 | None:intergenic | 15.0% |
| !!! | ATCAAATTACTATTCATTTT+TGG | + | chr1.3:4453618-4453637 | MS.gene67002:intron | 15.0% |
| !! | ACTATTTCAATGTTAACTTA+TGG | - | chr1.3:4453403-4453422 | None:intergenic | 20.0% |
| !! | ATAAATATTATGCAGATTAG+TGG | + | chr1.3:4453020-4453039 | MS.gene67002:intron | 20.0% |
| !! | CATAATTTACAAAGAAAATG+AGG | - | chr1.3:4453374-4453393 | None:intergenic | 20.0% |
| !! | CTATTTCAATGTTAACTTAT+GGG | - | chr1.3:4453402-4453421 | None:intergenic | 20.0% |
| !! | TAAATATTATGCAGATTAGT+GGG | + | chr1.3:4453021-4453040 | MS.gene67002:intron | 20.0% |
| !!! | AATTGGTAAGTTTTATTTCA+TGG | + | chr1.3:4452620-4452639 | MS.gene67002:intron | 20.0% |
| !!! | AGATTTTACTATCTTTTTAG+TGG | + | chr1.3:4452961-4452980 | MS.gene67002:intron | 20.0% |
| !!! | ATAGTAATTTGATAGCATTA+TGG | - | chr1.3:4453611-4453630 | None:intergenic | 20.0% |
| !!! | CTCATTTTCTTTGTAAATTA+TGG | + | chr1.3:4453372-4453391 | MS.gene67002:intron | 20.0% |
| !!! | GATTTTACTATCTTTTTAGT+GGG | + | chr1.3:4452962-4452981 | MS.gene67002:intron | 20.0% |
| ! | AAATATTATGCAGATTAGTG+GGG | + | chr1.3:4453022-4453041 | MS.gene67002:intron | 25.0% |
| ! | ACACAAAAGTTGTCAAAAAA+TGG | - | chr1.3:4453286-4453305 | None:intergenic | 25.0% |
| ! | ATTCTTCAATTGATCATGTA+AGG | + | chr1.3:4453127-4453146 | MS.gene67002:intron | 25.0% |
| ! | TTCTTCAATTGATCATGTAA+GGG | + | chr1.3:4453128-4453147 | MS.gene67002:intron | 25.0% |
| ! | TTTATAGTGACACTCAAAAT+GGG | + | chr1.3:4453076-4453095 | MS.gene67002:CDS | 25.0% |
| !! | AACATTTAGAATTTGCACAA+AGG | - | chr1.3:4453205-4453224 | None:intergenic | 25.0% |
| !! | GTTTGTTAGGATTATTGTAT+GGG | + | chr1.3:4452763-4452782 | MS.gene67002:intron | 25.0% |
| !! | TGTTTGTTAGGATTATTGTA+TGG | + | chr1.3:4452762-4452781 | MS.gene67002:intron | 25.0% |
| !!! | AATAACACGTTTTGTTTGTT+AGG | + | chr1.3:4452750-4452769 | MS.gene67002:intron | 25.0% |
| !!! | ATTTTAATGAATGTGAGCAA+AGG | + | chr1.3:4452801-4452820 | MS.gene67002:CDS | 25.0% |
| ACAACTTGGTTCATTTAACA+AGG | + | chr1.3:4453679-4453698 | MS.gene67002:CDS | 30.0% | |
| AGGTTTATAGTTATGGAGAA+GGG | + | chr1.3:4452882-4452901 | MS.gene67002:CDS | 30.0% | |
| ATTCTTCAAAATGTCTCATG+AGG | + | chr1.3:4452862-4452881 | MS.gene67002:CDS | 30.0% | |
| CCAAGGGAAATAAAAGAAAT+TGG | + | chr1.3:4452603-4452622 | MS.gene67002:CDS | 30.0% | |
| GTTTATAGTGACACTCAAAA+TGG | + | chr1.3:4453075-4453094 | MS.gene67002:CDS | 30.0% | |
| TCTCATGAGGTTTATAGTTA+TGG | + | chr1.3:4452875-4452894 | MS.gene67002:CDS | 30.0% | |
| TGATCAATTGAAGAATTCCA+TGG | - | chr1.3:4453123-4453142 | None:intergenic | 30.0% | |
| ! | ATTTAGAATTTGCACAAAGG+AGG | - | chr1.3:4453202-4453221 | None:intergenic | 30.0% |
| !! | CCAATTTCTTTTATTTCCCT+TGG | - | chr1.3:4452606-4452625 | None:intergenic | 30.0% |
| !! | TAAATTTTGACAGCAACCAA+GGG | + | chr1.3:4453444-4453463 | MS.gene67002:intron | 30.0% |
| !! | TTAAATTTTGACAGCAACCA+AGG | + | chr1.3:4453443-4453462 | MS.gene67002:intron | 30.0% |
| AAAGAAGGCTTAGTACAACT+TGG | + | chr1.3:4453665-4453684 | MS.gene67002:CDS | 35.0% | |
| AGGATTATTGTATGGGAAGA+TGG | + | chr1.3:4452770-4452789 | MS.gene67002:intron | 35.0% | |
| GAGGTTTATAGTTATGGAGA+AGG | + | chr1.3:4452881-4452900 | MS.gene67002:CDS | 35.0% | |
| GGATGCTTGAATGAAAGATT+TGG | + | chr1.3:4452830-4452849 | MS.gene67002:CDS | 35.0% | |
| TCAGTTAAATTCAGGCATTC+AGG | + | chr1.3:4453477-4453496 | MS.gene67002:CDS | 35.0% | |
| TGGGAGTTTCAGTTAAATTC+AGG | + | chr1.3:4453469-4453488 | MS.gene67002:CDS | 35.0% | |
| ! | AAACATGGCAAGAAAGTGTA+AGG | - | chr1.3:4452932-4452951 | None:intergenic | 35.0% |
| ! | AGCAGATAATGGTCACAAAT+GGG | + | chr1.3:4453053-4453072 | MS.gene67002:CDS | 35.0% |
| ACTTCTCTTGATCGTTCCAA+GGG | + | chr1.3:4452587-4452606 | MS.gene67002:CDS | 40.0% | |
| ATGCTAACTATGTTGGTCCA+TGG | + | chr1.3:4453103-4453122 | MS.gene67002:CDS | 40.0% | |
| GAATGTGAGCAAAGGAAGAA+TGG | + | chr1.3:4452809-4452828 | MS.gene67002:CDS | 40.0% | |
| ! | ATTCAGGCATTCAGGTTAGT+TGG | + | chr1.3:4453485-4453504 | MS.gene67002:intron | 40.0% |
| ! | CAGCAGATAATGGTCACAAA+TGG | + | chr1.3:4453052-4453071 | MS.gene67002:CDS | 40.0% |
| ! | TTCAGGCATTCAGGTTAGTT+GGG | + | chr1.3:4453486-4453505 | MS.gene67002:intron | 40.0% |
| GACTTCTCTTGATCGTTCCA+AGG | + | chr1.3:4452586-4452605 | MS.gene67002:CDS | 45.0% | |
| GCTGTCATTGCTGTCAAAGA+AGG | + | chr1.3:4453650-4453669 | MS.gene67002:CDS | 45.0% | |
| GGGAAAGTTGCAGCAGATAA+TGG | + | chr1.3:4453042-4453061 | MS.gene67002:CDS | 45.0% | |
| GGGTGTGATGCTAACTATGT+TGG | + | chr1.3:4453096-4453115 | MS.gene67002:CDS | 45.0% | |
| AACTGAAACTCCCATGCCCT+TGG | - | chr1.3:4453463-4453482 | None:intergenic | 50.0% | |
| TTTGACAGCAACCAAGGGCA+TGG | + | chr1.3:4453449-4453468 | MS.gene67002:intron | 50.0% | |
| ! | TTGACAGCAACCAAGGGCAT+GGG | + | chr1.3:4453450-4453469 | MS.gene67002:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 4452571 | 4453700 | 4452571 | ID=MS.gene67002 |
| chr1.3 | mRNA | 4452571 | 4453700 | 4452571 | ID=MS.gene67002.t1;Parent=MS.gene67002 |
| chr1.3 | exon | 4452571 | 4452624 | 4452571 | ID=MS.gene67002.t1.exon1;Parent=MS.gene67002.t1 |
| chr1.3 | CDS | 4452571 | 4452624 | 4452571 | ID=cds.MS.gene67002.t1;Parent=MS.gene67002.t1 |
| chr1.3 | exon | 4452772 | 4452903 | 4452772 | ID=MS.gene67002.t1.exon2;Parent=MS.gene67002.t1 |
| chr1.3 | CDS | 4452772 | 4452903 | 4452772 | ID=cds.MS.gene67002.t1;Parent=MS.gene67002.t1 |
| chr1.3 | exon | 4453035 | 4453143 | 4453035 | ID=MS.gene67002.t1.exon3;Parent=MS.gene67002.t1 |
| chr1.3 | CDS | 4453035 | 4453143 | 4453035 | ID=cds.MS.gene67002.t1;Parent=MS.gene67002.t1 |
| chr1.3 | exon | 4453457 | 4453498 | 4453457 | ID=MS.gene67002.t1.exon4;Parent=MS.gene67002.t1 |
| chr1.3 | CDS | 4453457 | 4453498 | 4453457 | ID=cds.MS.gene67002.t1;Parent=MS.gene67002.t1 |
| chr1.3 | exon | 4453644 | 4453700 | 4453644 | ID=MS.gene67002.t1.exon5;Parent=MS.gene67002.t1 |
| chr1.3 | CDS | 4453644 | 4453700 | 4453644 | ID=cds.MS.gene67002.t1;Parent=MS.gene67002.t1 |
| Gene Sequence |
| Protein sequence |
