Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69328.t1 | XP_013462271.1 | 97.7 | 222 | 5 | 0 | 1 | 222 | 1 | 222 | 4.50E-114 | 420.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69328.t1 | I3SCR3 | 97.7 | 222 | 5 | 0 | 1 | 222 | 1 | 222 | 3.2e-114 | 420.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049596 | MS.gene69328 | 0.823457 | 1.37E-53 | -1.69E-46 |
| MS.gene049989 | MS.gene69328 | 0.850626 | 1.50E-60 | -1.69E-46 |
| MS.gene050297 | MS.gene69328 | 0.850819 | 1.33E-60 | -1.69E-46 |
| MS.gene05124 | MS.gene69328 | 0.837748 | 4.33E-57 | -1.69E-46 |
| MS.gene051629 | MS.gene69328 | 0.84763 | 1.02E-59 | -1.69E-46 |
| MS.gene051816 | MS.gene69328 | 0.83698 | 6.81E-57 | -1.69E-46 |
| MS.gene051914 | MS.gene69328 | 0.802113 | 6.54E-49 | -1.69E-46 |
| MS.gene051955 | MS.gene69328 | 0.863163 | 3.03E-64 | -1.69E-46 |
| MS.gene052143 | MS.gene69328 | 0.81824 | 2.17E-52 | -1.69E-46 |
| MS.gene052308 | MS.gene69328 | 0.849482 | 3.14E-60 | -1.69E-46 |
| MS.gene052367 | MS.gene69328 | 0.88044 | 5.48E-70 | -1.69E-46 |
| MS.gene053173 | MS.gene69328 | 0.844517 | 7.20E-59 | -1.69E-46 |
| MS.gene053348 | MS.gene69328 | 0.825652 | 4.17E-54 | -1.69E-46 |
| MS.gene053416 | MS.gene69328 | 0.839583 | 1.45E-57 | -1.69E-46 |
| MS.gene053417 | MS.gene69328 | 0.838891 | 2.20E-57 | -1.69E-46 |
| MS.gene05369 | MS.gene69328 | 0.805934 | 1.05E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene69328 | 0.845481 | 3.95E-59 | -1.69E-46 |
| MS.gene054552 | MS.gene69328 | 0.821921 | 3.12E-53 | -1.69E-46 |
| MS.gene054593 | MS.gene69328 | 0.834037 | 3.78E-56 | -1.69E-46 |
| MS.gene054633 | MS.gene69328 | 0.808879 | 2.49E-50 | -1.69E-46 |
| MS.gene054643 | MS.gene69328 | 0.805684 | 1.19E-49 | -1.69E-46 |
| MS.gene054662 | MS.gene69328 | 0.814823 | 1.27E-51 | -1.69E-46 |
| MS.gene054885 | MS.gene69328 | 0.814804 | 1.28E-51 | -1.69E-46 |
| MS.gene055308 | MS.gene69328 | 0.839764 | 1.30E-57 | -1.69E-46 |
| MS.gene055578 | MS.gene69328 | 0.855401 | 6.47E-62 | -1.69E-46 |
| MS.gene055579 | MS.gene69328 | 0.802268 | 6.08E-49 | -1.69E-46 |
| MS.gene055957 | MS.gene69328 | 0.851392 | 9.15E-61 | -1.69E-46 |
| MS.gene056041 | MS.gene69328 | 0.886159 | 4.36E-72 | -1.69E-46 |
| MS.gene05640 | MS.gene69328 | 0.858173 | 9.88E-63 | -1.69E-46 |
| MS.gene056526 | MS.gene69328 | 0.818336 | 2.07E-52 | -1.69E-46 |
| MS.gene056528 | MS.gene69328 | 0.809113 | 2.22E-50 | -1.69E-46 |
| MS.gene057359 | MS.gene69328 | 0.800686 | 1.28E-48 | -1.69E-46 |
| MS.gene057458 | MS.gene69328 | 0.859194 | 4.89E-63 | -1.69E-46 |
| MS.gene058013 | MS.gene69328 | 0.81349 | 2.49E-51 | -1.69E-46 |
| MS.gene058128 | MS.gene69328 | 0.807296 | 5.42E-50 | -1.69E-46 |
| MS.gene058196 | MS.gene69328 | 0.800281 | 1.55E-48 | -1.69E-46 |
| MS.gene058818 | MS.gene69328 | 0.813828 | 2.10E-51 | -1.69E-46 |
| MS.gene058827 | MS.gene69328 | 0.81887 | 1.56E-52 | -1.69E-46 |
| MS.gene058844 | MS.gene69328 | 0.809589 | 1.76E-50 | -1.69E-46 |
| MS.gene058853 | MS.gene69328 | 0.844756 | 6.21E-59 | -1.69E-46 |
| MS.gene059300 | MS.gene69328 | 0.818439 | 1.96E-52 | -1.69E-46 |
| MS.gene059553 | MS.gene69328 | 0.860561 | 1.90E-63 | -1.69E-46 |
| MS.gene060473 | MS.gene69328 | 0.888615 | 5.05E-73 | -1.69E-46 |
| MS.gene060565 | MS.gene69328 | 0.879909 | 8.47E-70 | -1.69E-46 |
| MS.gene06057 | MS.gene69328 | 0.816887 | 4.39E-52 | -1.69E-46 |
| MS.gene06093 | MS.gene69328 | 0.856813 | 2.50E-62 | -1.69E-46 |
| MS.gene061099 | MS.gene69328 | 0.860952 | 1.44E-63 | -1.69E-46 |
| MS.gene061260 | MS.gene69328 | 0.880672 | 4.52E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69328.t1 | MTR_3g115300 | 97.748 | 222 | 5 | 0 | 1 | 222 | 1 | 222 | 7.65e-160 | 441 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69328.t1 | AT5G42330 | 47.208 | 197 | 83 | 10 | 6 | 194 | 2 | 185 | 4.86e-37 | 129 |
Find 53 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACAATTGCTTCTGAATTT+AGG | 0.081710 | 3.3:+94047620 | MS.gene69328:CDS |
| CACAATTGCTTCTGAATTTA+GGG | 0.195473 | 3.3:+94047621 | MS.gene69328:CDS |
| TGAAACAGAACACCAGATTT+AGG | 0.204443 | 3.3:-94047718 | None:intergenic |
| GAAGCAATTGTGCGGTATTC+AGG | 0.265276 | 3.3:-94047610 | None:intergenic |
| AGGATGGTTCGTGACGAATT+TGG | 0.279654 | 3.3:+94047652 | MS.gene69328:CDS |
| TCGGTCGCGGTGGTCGGAGG+TGG | 0.289193 | 3.3:-94047364 | None:intergenic |
| TGGTCGGAGGTGGGATTTCA+TGG | 0.291163 | 3.3:-94047354 | None:intergenic |
| GAAACAGAACACCAGATTTA+GGG | 0.318085 | 3.3:-94047717 | None:intergenic |
| CCAGATCCGGAAGAAATTAA+TGG | 0.326179 | 3.3:+94047679 | MS.gene69328:CDS |
| CCATTAATTTCTTCCGGATC+TGG | 0.333300 | 3.3:-94047679 | None:intergenic |
| TTTAACCAAGTCTCGGAAAC+TGG | 0.342643 | 3.3:+94047567 | MS.gene69328:CDS |
| AGATTTAGGGTTACAAGAAT+TGG | 0.342948 | 3.3:-94047704 | None:intergenic |
| CTTGGTGTTACTGTTGAATC+TGG | 0.346379 | 3.3:+94047871 | MS.gene69328:CDS |
| TTGGTACCATTAATTTCTTC+CGG | 0.348296 | 3.3:-94047685 | None:intergenic |
| ATGATGAGTATTCTGCAAAA+AGG | 0.356084 | 3.3:+94047755 | MS.gene69328:CDS |
| CGAAGACGCATCGGAGACTT+AGG | 0.358488 | 3.3:-94047389 | None:intergenic |
| CGGTATTCAGGTCGTAGTTC+AGG | 0.361330 | 3.3:-94047598 | None:intergenic |
| AATAATCATCATAATGTTCT+TGG | 0.387259 | 3.3:+94047853 | MS.gene69328:CDS |
| AATTCTTGTAACCCTAAATC+TGG | 0.396987 | 3.3:+94047706 | MS.gene69328:CDS |
| AAGAATACTGCAAGAAAACT+TGG | 0.439698 | 3.3:+94047898 | MS.gene69328:CDS |
| CCCTCTCTTCAAACTCCACC+AGG | 0.453304 | 3.3:+94047434 | MS.gene69328:CDS |
| GAGAGGTTGAAGAGAAAGAA+AGG | 0.462625 | 3.3:+94047781 | MS.gene69328:CDS |
| TAGGGATCGGTCGCGGTGGT+CGG | 0.470489 | 3.3:-94047370 | None:intergenic |
| TTGAACCAGTTTCCGAGACT+TGG | 0.470567 | 3.3:-94047572 | None:intergenic |
| CGGTCGCGGTGGTCGGAGGT+GGG | 0.481693 | 3.3:-94047363 | None:intergenic |
| CTGAATTTAGGGCTTTATCA+AGG | 0.492327 | 3.3:+94047632 | MS.gene69328:CDS |
| CGAATTTGGAAAACCAGATC+CGG | 0.501998 | 3.3:+94047666 | MS.gene69328:CDS |
| AATAAGAAACCTCCTCTAGC+TGG | 0.510440 | 3.3:+94048006 | MS.gene69328:CDS |
| TACTAGTATTAAGCAAACTA+AGG | 0.520435 | 3.3:+94047828 | MS.gene69328:CDS |
| GTTGGAGCATCGAGAAGAGT+AGG | 0.523400 | 3.3:+94048069 | MS.gene69328:CDS |
| ACTGTTGGTGAGAAGAAAGT+TGG | 0.529021 | 3.3:+94048051 | MS.gene69328:CDS |
| GGATCGGTCGCGGTGGTCGG+AGG | 0.529657 | 3.3:-94047367 | None:intergenic |
| ATTTAGGGCTTTATCAAGGA+TGG | 0.529903 | 3.3:+94047636 | MS.gene69328:CDS |
| CCTGGTGGAGTTTGAAGAGA+GGG | 0.535099 | 3.3:-94047434 | None:intergenic |
| CATGCTTAGAAGCATGACTA+AGG | 0.538545 | 3.3:+94047981 | MS.gene69328:CDS |
| CAGGTTCTTTAACCAAGTCT+CGG | 0.545491 | 3.3:+94047560 | MS.gene69328:intron |
| TTCTGCTGCTTATTCTGCTG+AGG | 0.545660 | 3.3:+94047939 | MS.gene69328:CDS |
| TTTGATAAATCTGTTACTGT+TGG | 0.555181 | 3.3:+94048036 | MS.gene69328:CDS |
| ACCTGGTGGAGTTTGAAGAG+AGG | 0.555239 | 3.3:-94047435 | None:intergenic |
| GAAGACGCATCGGAGACTTA+GGG | 0.556071 | 3.3:-94047388 | None:intergenic |
| TCAAATCTTGAACCAGCTAG+AGG | 0.605228 | 3.3:-94048018 | None:intergenic |
| CGCATCGGAGACTTAGGGAT+CGG | 0.618390 | 3.3:-94047383 | None:intergenic |
| ATGCTTCTAAGCATGTACCT+TGG | 0.620137 | 3.3:-94047973 | None:intergenic |
| AAGAGAGGGAGAGTCAAGAA+CGG | 0.623596 | 3.3:-94047420 | None:intergenic |
| GTTCGAGTTCGAAGACGCAT+CGG | 0.624113 | 3.3:-94047398 | None:intergenic |
| GGAGACTTAGGGATCGGTCG+CGG | 0.628168 | 3.3:-94047377 | None:intergenic |
| AATCTTGAACCAGCTAGAGG+AGG | 0.634666 | 3.3:-94048015 | None:intergenic |
| CTGAGGTTAGTGAAACTCCA+AGG | 0.641822 | 3.3:+94047956 | MS.gene69328:CDS |
| GACTTAGGGATCGGTCGCGG+TGG | 0.645567 | 3.3:-94047374 | None:intergenic |
| GAAGAGAAAGAAAGGAGTCA+CGG | 0.667345 | 3.3:+94047789 | MS.gene69328:CDS |
| TCTGGTGTTCTGTTTCAGAG+AGG | 0.698623 | 3.3:+94047724 | MS.gene69328:CDS |
| TAAATTCAGAAGCAATTGTG+CGG | 0.713706 | 3.3:-94047618 | None:intergenic |
| GAGAAAGAAAGGAGTCACGG+TGG | 0.729465 | 3.3:+94047792 | MS.gene69328:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATCATCATAATGTTCT+TGG | + | chr3.3:94047853-94047872 | MS.gene69328:CDS | 20.0% |
| ! | GAAATAAGATTATAAGAACC+TGG | - | chr3.3:94047455-94047474 | None:intergenic | 25.0% |
| ! | TACTAGTATTAAGCAAACTA+AGG | + | chr3.3:94047828-94047847 | MS.gene69328:CDS | 25.0% |
| ! | TTTGATAAATCTGTTACTGT+TGG | + | chr3.3:94048036-94048055 | MS.gene69328:CDS | 25.0% |
| AAGAATACTGCAAGAAAACT+TGG | + | chr3.3:94047898-94047917 | MS.gene69328:CDS | 30.0% | |
| AATTCTTGTAACCCTAAATC+TGG | + | chr3.3:94047706-94047725 | MS.gene69328:CDS | 30.0% | |
| ATAAGATTATAAGAACCTGG+TGG | - | chr3.3:94047452-94047471 | None:intergenic | 30.0% | |
| ATGATGAGTATTCTGCAAAA+AGG | + | chr3.3:94047755-94047774 | MS.gene69328:CDS | 30.0% | |
| CACAATTGCTTCTGAATTTA+GGG | + | chr3.3:94047621-94047640 | MS.gene69328:CDS | 30.0% | |
| CCACAAACAAAAAATGTCAA+TGG | - | chr3.3:94047525-94047544 | None:intergenic | 30.0% | |
| GTGGATTTGAAAATTGAAAC+AGG | + | chr3.3:94047541-94047560 | MS.gene69328:intron | 30.0% | |
| TAAATTCAGAAGCAATTGTG+CGG | - | chr3.3:94047621-94047640 | None:intergenic | 30.0% | |
| TTGGTACCATTAATTTCTTC+CGG | - | chr3.3:94047688-94047707 | None:intergenic | 30.0% | |
| ! | AGATTTAGGGTTACAAGAAT+TGG | - | chr3.3:94047707-94047726 | None:intergenic | 30.0% |
| !!! | CCATTGACATTTTTTGTTTG+TGG | + | chr3.3:94047522-94047541 | MS.gene69328:intron | 30.0% |
| GAAACAGAACACCAGATTTA+GGG | - | chr3.3:94047720-94047739 | None:intergenic | 35.0% | |
| GCACAATTGCTTCTGAATTT+AGG | + | chr3.3:94047620-94047639 | MS.gene69328:CDS | 35.0% | |
| TGAAACAGAACACCAGATTT+AGG | - | chr3.3:94047721-94047740 | None:intergenic | 35.0% | |
| ! | ATTTAGGGCTTTATCAAGGA+TGG | + | chr3.3:94047636-94047655 | MS.gene69328:CDS | 35.0% |
| ! | CTGAATTTAGGGCTTTATCA+AGG | + | chr3.3:94047632-94047651 | MS.gene69328:CDS | 35.0% |
| AATAAGAAACCTCCTCTAGC+TGG | + | chr3.3:94048006-94048025 | MS.gene69328:CDS | 40.0% | |
| ATGCTTCTAAGCATGTACCT+TGG | - | chr3.3:94047976-94047995 | None:intergenic | 40.0% | |
| ATTCTGCAAAAAGGAACGAG+AGG | + | chr3.3:94047764-94047783 | MS.gene69328:CDS | 40.0% | |
| CAGGTTCTTTAACCAAGTCT+CGG | + | chr3.3:94047560-94047579 | MS.gene69328:intron | 40.0% | |
| CCAGATCCGGAAGAAATTAA+TGG | + | chr3.3:94047679-94047698 | MS.gene69328:CDS | 40.0% | |
| CCATTAATTTCTTCCGGATC+TGG | - | chr3.3:94047682-94047701 | None:intergenic | 40.0% | |
| CGAATTTGGAAAACCAGATC+CGG | + | chr3.3:94047666-94047685 | MS.gene69328:CDS | 40.0% | |
| GAAGAGAAAGAAAGGAGTCA+CGG | + | chr3.3:94047789-94047808 | MS.gene69328:CDS | 40.0% | |
| GAGAGGTTGAAGAGAAAGAA+AGG | + | chr3.3:94047781-94047800 | MS.gene69328:CDS | 40.0% | |
| TCAAATCTTGAACCAGCTAG+AGG | - | chr3.3:94048021-94048040 | None:intergenic | 40.0% | |
| TTTAACCAAGTCTCGGAAAC+TGG | + | chr3.3:94047567-94047586 | MS.gene69328:CDS | 40.0% | |
| ! | ACTGTTGGTGAGAAGAAAGT+TGG | + | chr3.3:94048051-94048070 | MS.gene69328:CDS | 40.0% |
| ! | CATGCTTAGAAGCATGACTA+AGG | + | chr3.3:94047981-94048000 | MS.gene69328:CDS | 40.0% |
| !! | CTTGGTGTTACTGTTGAATC+TGG | + | chr3.3:94047871-94047890 | MS.gene69328:CDS | 40.0% |
| AAGAGAGGGAGAGTCAAGAA+CGG | - | chr3.3:94047423-94047442 | None:intergenic | 45.0% | |
| AATCTTGAACCAGCTAGAGG+AGG | - | chr3.3:94048018-94048037 | None:intergenic | 45.0% | |
| AGGATGGTTCGTGACGAATT+TGG | + | chr3.3:94047652-94047671 | MS.gene69328:CDS | 45.0% | |
| CTGAGGTTAGTGAAACTCCA+AGG | + | chr3.3:94047956-94047975 | MS.gene69328:CDS | 45.0% | |
| GAAGCAATTGTGCGGTATTC+AGG | - | chr3.3:94047613-94047632 | None:intergenic | 45.0% | |
| TCTGGTGTTCTGTTTCAGAG+AGG | + | chr3.3:94047724-94047743 | MS.gene69328:CDS | 45.0% | |
| TTGAACCAGTTTCCGAGACT+TGG | - | chr3.3:94047575-94047594 | None:intergenic | 45.0% | |
| ! | TTCTGCTGCTTATTCTGCTG+AGG | + | chr3.3:94047939-94047958 | MS.gene69328:CDS | 45.0% |
| ACCTGGTGGAGTTTGAAGAG+AGG | - | chr3.3:94047438-94047457 | None:intergenic | 50.0% | |
| CCTGGTGGAGTTTGAAGAGA+GGG | - | chr3.3:94047437-94047456 | None:intergenic | 50.0% | |
| CGGTATTCAGGTCGTAGTTC+AGG | - | chr3.3:94047601-94047620 | None:intergenic | 50.0% | |
| GAAGACGCATCGGAGACTTA+GGG | - | chr3.3:94047391-94047410 | None:intergenic | 50.0% | |
| GAGAAAGAAAGGAGTCACGG+TGG | + | chr3.3:94047792-94047811 | MS.gene69328:CDS | 50.0% | |
| ! | GTTCGAGTTCGAAGACGCAT+CGG | - | chr3.3:94047401-94047420 | None:intergenic | 50.0% |
| !! | GTTGGAGCATCGAGAAGAGT+AGG | + | chr3.3:94048069-94048088 | MS.gene69328:CDS | 50.0% |
| CCCTCTCTTCAAACTCCACC+AGG | + | chr3.3:94047434-94047453 | MS.gene69328:CDS | 55.0% | |
| CGAAGACGCATCGGAGACTT+AGG | - | chr3.3:94047392-94047411 | None:intergenic | 55.0% | |
| CGCATCGGAGACTTAGGGAT+CGG | - | chr3.3:94047386-94047405 | None:intergenic | 55.0% | |
| TGGTCGGAGGTGGGATTTCA+TGG | - | chr3.3:94047357-94047376 | None:intergenic | 55.0% | |
| GGAGACTTAGGGATCGGTCG+CGG | - | chr3.3:94047380-94047399 | None:intergenic | 60.0% | |
| GACTTAGGGATCGGTCGCGG+TGG | - | chr3.3:94047377-94047396 | None:intergenic | 65.0% | |
| TAGGGATCGGTCGCGGTGGT+CGG | - | chr3.3:94047373-94047392 | None:intergenic | 65.0% | |
| CGGTCGCGGTGGTCGGAGGT+GGG | - | chr3.3:94047366-94047385 | None:intergenic | 75.0% | |
| GGATCGGTCGCGGTGGTCGG+AGG | - | chr3.3:94047370-94047389 | None:intergenic | 75.0% | |
| TCGGTCGCGGTGGTCGGAGG+TGG | - | chr3.3:94047367-94047386 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 94047335 | 94048110 | 94047335 | ID=MS.gene69328 |
| chr3.3 | mRNA | 94047335 | 94048110 | 94047335 | ID=MS.gene69328.t1;Parent=MS.gene69328 |
| chr3.3 | exon | 94047335 | 94047455 | 94047335 | ID=MS.gene69328.t1.exon1;Parent=MS.gene69328.t1 |
| chr3.3 | CDS | 94047335 | 94047455 | 94047335 | ID=cds.MS.gene69328.t1;Parent=MS.gene69328.t1 |
| chr3.3 | exon | 94047563 | 94048110 | 94047563 | ID=MS.gene69328.t1.exon2;Parent=MS.gene69328.t1 |
| chr3.3 | CDS | 94047563 | 94048110 | 94047563 | ID=cds.MS.gene69328.t1;Parent=MS.gene69328.t1 |
| Gene Sequence |
| Protein sequence |
