AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73638


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73638.t1 MTR_4g051652 92.857 210 15 0 3 212 1 210 1.04e-144 402
MS.gene73638.t1 MTR_2g087080 53.846 208 90 3 5 206 4 211 6.07e-69 210
MS.gene73638.t1 MTR_4g051672 54.930 213 23 5 3 212 1 143 6.08e-64 195
MS.gene73638.t1 MTR_4g051705 89.888 89 9 0 124 212 1 89 5.86e-54 167
MS.gene73638.t1 MTR_4g051745 82.022 89 4 1 124 212 1 77 5.67e-45 144
MS.gene73638.t1 MTR_2g087000 27.136 199 138 3 9 207 39 230 2.14e-22 91.3
MS.gene73638.t1 MTR_2g011350 27.273 154 108 3 41 193 41 191 1.32e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73638.t1 AT3G52460 29.897 194 125 4 14 199 99 289 1.92e-17 79.3
MS.gene73638.t1 AT5G22870 25.389 193 133 6 3 187 1 190 1.83e-11 61.6
MS.gene73638.t1 AT2G27260 27.329 161 112 4 46 205 84 240 3.41e-11 61.2

Find 53 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGATGTGGAGATACTTTA+CGG 0.300931 4.1:-46456763 MS.gene73638:CDS
AAAAGACACTGGAAAACTAT+TGG 0.316716 4.1:-46456467 MS.gene73638:CDS
TTCAATAACTAACTATGGTT+TGG 0.319426 4.1:+46456414 None:intergenic
TTGGATTTGAAGCTGGAATA+TGG 0.322794 4.1:-46456552 MS.gene73638:CDS
CTGGAAAACTATTGGGTATT+GGG 0.345902 4.1:-46456459 MS.gene73638:CDS
ATGTGTTTCTTGTGGGGACT+TGG 0.423348 4.1:-46456509 MS.gene73638:CDS
ACTGGAAAACTATTGGGTAT+TGG 0.428456 4.1:-46456460 MS.gene73638:CDS
AAAGACACTGGAAAACTATT+GGG 0.440360 4.1:-46456466 MS.gene73638:CDS
GGTGCAACAACCTATACTTT+TGG 0.440362 4.1:-46456716 MS.gene73638:CDS
TGGCCATCATTGGCATAGCT+TGG 0.441176 4.1:-46456936 MS.gene73638:CDS
CTTCCGACACCGGCTTTGTC+CGG 0.445859 4.1:-46456999 MS.gene73638:CDS
TTCTTGTGGGGACTTGGATG+TGG 0.458190 4.1:-46456503 MS.gene73638:CDS
GTTAGAGTTGGATTTGAAGC+TGG 0.458770 4.1:-46456559 MS.gene73638:CDS
CTCATCTTCACCAAAAGTAT+AGG 0.463962 4.1:+46456706 None:intergenic
GTGAAGATGGTTGTTAGAGT+TGG 0.466909 4.1:-46456571 MS.gene73638:CDS
ACAGATTACAGGCGTGTTCG+AGG 0.475631 4.1:-46456839 MS.gene73638:CDS
AGTGATGTTACCGCAAAACT+AGG 0.482969 4.1:+46456892 None:intergenic
GTTGGGATATTTCTAGTAGG+AGG 0.485932 4.1:+46457027 None:intergenic
CAAGAGTATGAAGGTTGACT+TGG 0.487527 4.1:-46456680 MS.gene73638:CDS
CGGTGTCGGAAGATCTTGTT+GGG 0.487634 4.1:+46457010 None:intergenic
AAGACATTATGTGTTTCTTG+TGG 0.490786 4.1:-46456517 MS.gene73638:CDS
TTGGGATATTTCTAGTAGGA+GGG 0.498040 4.1:+46457028 None:intergenic
CTTGTTGGGATATTTCTAGT+AGG 0.499013 4.1:+46457024 None:intergenic
CACCTCAGCTTCCGAGAGCA+CGG 0.502696 4.1:+46456735 None:intergenic
AGACATTATGTGTTTCTTGT+GGG 0.505280 4.1:-46456516 MS.gene73638:CDS
TTACGGTGAAACCGTGCTCT+CGG 0.510617 4.1:-46456746 MS.gene73638:CDS
GTATTCGATTCACAGATTAC+AGG 0.518278 4.1:-46456850 MS.gene73638:CDS
TAGTTGCGGAGTGGAACAAA+GGG 0.526316 4.1:-46456609 MS.gene73638:CDS
CCGGTGTCGGAAGATCTTGT+TGG 0.532501 4.1:+46457009 None:intergenic
AAACCAAGCTATGCCAATGA+TGG 0.532510 4.1:+46456933 None:intergenic
ATTACAGGCGTGTTCGAGGC+GGG 0.548697 4.1:-46456835 MS.gene73638:CDS
GACATTATGTGTTTCTTGTG+GGG 0.561691 4.1:-46456515 MS.gene73638:CDS
GGTTAGACGCCGGACAAAGC+CGG 0.562191 4.1:+46456990 None:intergenic
TTCAACGAAGTTCTTGCAGA+AGG 0.562601 4.1:-46456647 MS.gene73638:CDS
TTCTATTCTACAAAAGACAC+TGG 0.567750 4.1:-46456478 MS.gene73638:CDS
ACGCCGGACAAAGCCGGTGT+CGG 0.579838 4.1:+46456996 None:intergenic
CTAGTTGCGGAGTGGAACAA+AGG 0.586760 4.1:-46456610 MS.gene73638:CDS
GATGAGCAACAAGAGTATGA+AGG 0.586934 4.1:-46456689 MS.gene73638:CDS
AACCGTGCTCTCGGAAGCTG+AGG 0.589161 4.1:-46456737 MS.gene73638:CDS
AGCTGGAATATGGCCAAGCA+AGG 0.592271 4.1:-46456542 MS.gene73638:CDS
GTGATGTTACCGCAAAACTA+GGG 0.599545 4.1:+46456893 None:intergenic
AACGAAGTTCTTGCAGAAGG+CGG 0.601484 4.1:-46456644 MS.gene73638:CDS
AAAGATTGATACAACTGTCA+AGG 0.607863 4.1:+46456969 None:intergenic
CCAACAAGATCTTCCGACAC+CGG 0.612704 4.1:-46457009 MS.gene73638:CDS
AAAATCCAAAGTAATGAACA+TGG 0.633536 4.1:-46457055 None:intergenic
ACCGCAAAACTAGGGTCGTG+TGG 0.643655 4.1:+46456901 None:intergenic
GGTTCTCGATAATTTCGATG+TGG 0.655826 4.1:-46456776 MS.gene73638:CDS
TGCGGAGTGGAACAAAGGGG+TGG 0.672319 4.1:-46456605 MS.gene73638:CDS
GGTGCAAGAGAATCTAGTTG+CGG 0.677413 4.1:-46456623 MS.gene73638:CDS
GATTACAGGCGTGTTCGAGG+CGG 0.683167 4.1:-46456836 MS.gene73638:CDS
AAGAGAATCTAGTTGCGGAG+TGG 0.723007 4.1:-46456618 MS.gene73638:CDS
CAACTGTCAAGGTTAGACGC+CGG 0.742495 4.1:+46456980 None:intergenic
AGTTGCGGAGTGGAACAAAG+GGG 0.746823 4.1:-46456608 MS.gene73638:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTTTGGTTTGTTA+TGG + chr4.1:46456664-46456683 None:intergenic 20.0%
! CCAAAACAAAAAAATCGAAT+TGG - chr4.1:46456671-46456690 MS.gene73638:CDS 25.0%
!!! ATCAATCTTTGTCATTTTCA+TGG - chr4.1:46456512-46456531 MS.gene73638:CDS 25.0%
!!! CCAATTCGATTTTTTTGTTT+TGG + chr4.1:46456674-46456693 None:intergenic 25.0%
AAAAGACACTGGAAAACTAT+TGG - chr4.1:46457001-46457020 MS.gene73638:CDS 30.0%
AAAGACACTGGAAAACTATT+GGG - chr4.1:46457002-46457021 MS.gene73638:CDS 30.0%
AAAGATTGATACAACTGTCA+AGG + chr4.1:46456502-46456521 None:intergenic 30.0%
AAGACATTATGTGTTTCTTG+TGG - chr4.1:46456951-46456970 MS.gene73638:CDS 30.0%
AGACATTATGTGTTTCTTGT+GGG - chr4.1:46456952-46456971 MS.gene73638:CDS 30.0%
TTCTATTCTACAAAAGACAC+TGG - chr4.1:46456990-46457009 MS.gene73638:CDS 30.0%
ACTGGAAAACTATTGGGTAT+TGG - chr4.1:46457008-46457027 MS.gene73638:CDS 35.0%
CTGGAAAACTATTGGGTATT+GGG - chr4.1:46457009-46457028 MS.gene73638:CDS 35.0%
GACATTATGTGTTTCTTGTG+GGG - chr4.1:46456953-46456972 MS.gene73638:CDS 35.0%
GTATTCGATTCACAGATTAC+AGG - chr4.1:46456618-46456637 MS.gene73638:CDS 35.0%
TTCGATGTGGAGATACTTTA+CGG - chr4.1:46456705-46456724 MS.gene73638:CDS 35.0%
! CATAATGTCTTTTCCTTGCT+TGG + chr4.1:46456942-46456961 None:intergenic 35.0%
! CTCATCTTCACCAAAAGTAT+AGG + chr4.1:46456765-46456784 None:intergenic 35.0%
! TTGGATTTGAAGCTGGAATA+TGG - chr4.1:46456916-46456935 MS.gene73638:CDS 35.0%
!! CTTGTTGGGATATTTCTAGT+AGG + chr4.1:46456447-46456466 None:intergenic 35.0%
!! GGTGAATTTTGATGTGAAGA+TGG - chr4.1:46456884-46456903 MS.gene73638:CDS 35.0%
!! TTGGGATATTTCTAGTAGGA+GGG + chr4.1:46456443-46456462 None:intergenic 35.0%
AAACCAAGCTATGCCAATGA+TGG + chr4.1:46456538-46456557 None:intergenic 40.0%
AGTGATGTTACCGCAAAACT+AGG + chr4.1:46456579-46456598 None:intergenic 40.0%
GGTTCTCGATAATTTCGATG+TGG - chr4.1:46456692-46456711 MS.gene73638:CDS 40.0%
GTGAAGATGGTTGTTAGAGT+TGG - chr4.1:46456897-46456916 MS.gene73638:CDS 40.0%
GTGATGTTACCGCAAAACTA+GGG + chr4.1:46456578-46456597 None:intergenic 40.0%
TTCAACGAAGTTCTTGCAGA+AGG - chr4.1:46456821-46456840 MS.gene73638:CDS 40.0%
! CAAGAGTATGAAGGTTGACT+TGG - chr4.1:46456788-46456807 MS.gene73638:CDS 40.0%
! GATGAGCAACAAGAGTATGA+AGG - chr4.1:46456779-46456798 MS.gene73638:CDS 40.0%
! GGTGCAACAACCTATACTTT+TGG - chr4.1:46456752-46456771 MS.gene73638:CDS 40.0%
! GTTAGAGTTGGATTTGAAGC+TGG - chr4.1:46456909-46456928 MS.gene73638:CDS 40.0%
!! GTCATTTTCATGGCCATCAT+TGG - chr4.1:46456522-46456541 MS.gene73638:CDS 40.0%
!! GTTGGGATATTTCTAGTAGG+AGG + chr4.1:46456444-46456463 None:intergenic 40.0%
AACGAAGTTCTTGCAGAAGG+CGG - chr4.1:46456824-46456843 MS.gene73638:CDS 45.0%
AAGAGAATCTAGTTGCGGAG+TGG - chr4.1:46456850-46456869 MS.gene73638:CDS 45.0%
ATGTGTTTCTTGTGGGGACT+TGG - chr4.1:46456959-46456978 MS.gene73638:CDS 45.0%
GGTGCAAGAGAATCTAGTTG+CGG - chr4.1:46456845-46456864 MS.gene73638:CDS 45.0%
TAGTTGCGGAGTGGAACAAA+GGG - chr4.1:46456859-46456878 MS.gene73638:CDS 45.0%
ACAGATTACAGGCGTGTTCG+AGG - chr4.1:46456629-46456648 MS.gene73638:CDS 50.0%
AGCTGGAATATGGCCAAGCA+AGG - chr4.1:46456926-46456945 MS.gene73638:CDS 50.0%
AGTTGCGGAGTGGAACAAAG+GGG - chr4.1:46456860-46456879 MS.gene73638:CDS 50.0%
CAACTGTCAAGGTTAGACGC+CGG + chr4.1:46456491-46456510 None:intergenic 50.0%
CCAACAAGATCTTCCGACAC+CGG - chr4.1:46456459-46456478 MS.gene73638:CDS 50.0%
CTAGTTGCGGAGTGGAACAA+AGG - chr4.1:46456858-46456877 MS.gene73638:CDS 50.0%
TGGCCATCATTGGCATAGCT+TGG - chr4.1:46456532-46456551 MS.gene73638:CDS 50.0%
TTACGGTGAAACCGTGCTCT+CGG - chr4.1:46456722-46456741 MS.gene73638:CDS 50.0%
TTCTTGTGGGGACTTGGATG+TGG - chr4.1:46456965-46456984 MS.gene73638:CDS 50.0%
! CGGTGTCGGAAGATCTTGTT+GGG + chr4.1:46456461-46456480 None:intergenic 50.0%
!!! TCCACACGACCCTAGTTTTG+CGG - chr4.1:46456566-46456585 MS.gene73638:CDS 50.0%
ACCGCAAAACTAGGGTCGTG+TGG + chr4.1:46456570-46456589 None:intergenic 55.0%
ATTACAGGCGTGTTCGAGGC+GGG - chr4.1:46456633-46456652 MS.gene73638:CDS 55.0%
GATTACAGGCGTGTTCGAGG+CGG - chr4.1:46456632-46456651 MS.gene73638:CDS 55.0%
! CCGGTGTCGGAAGATCTTGT+TGG + chr4.1:46456462-46456481 None:intergenic 55.0%
AACCGTGCTCTCGGAAGCTG+AGG - chr4.1:46456731-46456750 MS.gene73638:CDS 60.0%
CACCTCAGCTTCCGAGAGCA+CGG + chr4.1:46456736-46456755 None:intergenic 60.0%
GGTTAGACGCCGGACAAAGC+CGG + chr4.1:46456481-46456500 None:intergenic 60.0%
TGCGGAGTGGAACAAAGGGG+TGG - chr4.1:46456863-46456882 MS.gene73638:CDS 60.0%
!! CTTCCGACACCGGCTTTGTC+CGG - chr4.1:46456469-46456488 MS.gene73638:CDS 60.0%
ACGCCGGACAAAGCCGGTGT+CGG + chr4.1:46456475-46456494 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 46456426 46457064 46456426 ID=MS.gene73638
chr4.1 mRNA 46456426 46457064 46456426 ID=MS.gene73638.t1;Parent=MS.gene73638
chr4.1 exon 46456426 46457064 46456426 ID=MS.gene73638.t1.exon1;Parent=MS.gene73638.t1
chr4.1 CDS 46456426 46457064 46456426 ID=cds.MS.gene73638.t1;Parent=MS.gene73638.t1
Gene Sequence

>MS.gene73638

ATGAACATGGAGCACCCTCCTACTAGAAATATCCCAACAAGATCTTCCGACACCGGCTTTGTCCGGCGTCTAACCTTGACAGTTGTATCAATCTTTGTCATTTTCATGGCCATCATTGGCATAGCTTGGTTTGTTATGCATCCACACGACCCTAGTTTTGCGGTAACATCACTTTCTGTCTCAAACTTCACTGTATTCGATTCACAGATTACAGGCGTGTTCGAGGCGGGACTAACCATAACAAACCAAAACAAAAAAATCGAATTGGTTCTCGATAATTTCGATGTGGAGATACTTTACGGTGAAACCGTGCTCTCGGAAGCTGAGGTGCAACAACCTATACTTTTGGTGAAGATGAGCAACAAGAGTATGAAGGTTGACTTGGTAACGACGAATTCAACGAAGTTCTTGCAGAAGGCGGTGCAAGAGAATCTAGTTGCGGAGTGGAACAAAGGGGTGGTGAATTTTGATGTGAAGATGGTTGTTAGAGTTGGATTTGAAGCTGGAATATGGCCAAGCAAGGAAAAGACATTATGTGTTTCTTGTGGGGACTTGGATGTGGAATTCTATTCTACAAAAGACACTGGAAAACTATTGGGTATTGGGAAGAACTGCAACATAGTTGAAGCCAAACCATAG

Protein sequence

>MS.gene73638.t1

MNMEHPPTRNIPTRSSDTGFVRRLTLTVVSIFVIFMAIIGIAWFVMHPHDPSFAVTSLSVSNFTVFDSQITGVFEAGLTITNQNKKIELVLDNFDVEILYGETVLSEAEVQQPILLVKMSNKSMKVDLVTTNSTKFLQKAVQENLVAEWNKGVVNFDVKMVVRVGFEAGIWPSKEKTLCVSCGDLDVEFYSTKDTGKLLGIGKNCNIVEAKP